MD04G1089500.v1.1

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001343
Physical Location & Seq
Reverse (-)
15729349 .. 15732132
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1089500.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGATGTTTGAATCAAAGCAAACTCATCTTCGGTTCTTTGCTTTAACAATTATTCTTCTGTACTTGCACCCTATAGCACTTACAGCTAAACCACTTGACCAAGAAATAGAGTTTGATTATAGAGAAGGAAGCGAGAAGGGGCCACAACACTGGGGAGAGCTTAAGAAAGAATGGACGGCATGCAAAGATGGAAAATCTCAATCACCAATAGACTTAATGGAGGATGGTGCCACCAAAGTGATCCCAACTTTTAGGGATCTTACAATGCACCATAAGCCTTCTGGTGCAACTCTCAAGAATGAAGGTCACTATATCGCGCTTGAATGGGAAGGTGATGCTGGATCTATCCAAATCAACGGCACAAATTACTTCCTCAAACAATGTCATTGGCACACCCCATCCGAGCACACCATCAATGGCATAAGGTATGATCTAGAATTGCACATGGTACACCGAAGTGCCGACAAGAACAGTGTAGCTGTAATTGCCTTCCTCTACCAAGTTGGTCCTCCCAATCCTTTTCTCTTAAAGGTGAGCAAGGATATACTGTCTGTGATTGGTACGAAGGAGAAACACTTGGGAGTGATTAATCCAAGTGAAATAAAATGGCCTAGCTTAAGATTTTACAGATACGTGGGATCACTCACAACCCCTCCCTGTACTGAAGCAGTTATTTGGACCGTAAACGAAGGGGTAAGTATTGTTTCAAGAAGACAACTGGAGTTACTTAAACAAGTAGTTGATGATTATGCAGTGATGAATGCAAGGCCATTGCAACCTACCAATGACCGAGGTATCAAGCTCTATGGCTCGACATCATGGTTAAGTGATGACAAAGTATCTCATCACACAACGATTAATGCTGCATCAAGCCCTCAAGAGTCGATCAATGCAGCGAGTGCACAAGAGAGTAGCCACAGCCCCAAACAGGGTAACCACAGTCCCCAACAGACTGTTAGACTATTTCCGCAAGTAATCGCGTGTCTGTTCGTGTGTCTTCTCCACTTGGTTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

340

Amino Acids

38.16

Weight (kDa)

6.78

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 46 - 267 9.2e-40 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 227
AccII CGCG 2 cut(s) 317, 980
AciI CCGC 1 cut(s) 968
AclWI GGATC 4 cut(s) 235, 264, 349, 646
AcuI CTGAAG 1 cut(s) 684
AfaI GTAC 4 cut(s) 62, 450, 562, 661
AfiI CCNNNNNNNGG 2 cut(s) 928, 929
AflII CTTAAG 2 cut(s) 161, 616
AgsI TTSAA 3 cut(s) 11, 323, 708
AleI CACNNNNGTG 2 cut(s) 236, 456
AluBI AGCT 5 cut(s) 86, 160, 479, 615, 802
AluI AGCT 5 cut(s) 86, 160, 479, 615, 802
Alw21I GWGCWC 2 cut(s) 408, 904
Alw44I GTGCAC 1 cut(s) 900
AlwI GGATC 4 cut(s) 235, 264, 349, 646
AoxI GGCC 3 cut(s) 140, 608, 767
ApaLI GTGCAC 1 cut(s) 900
ApeKI GCWGC 2 cut(s) 863, 893
AseI ATTAAT 3 cut(s) 588, 858, 1014
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 3 cut(s) 140, 506, 678
AsuHPI GGTGA 3 cut(s) 195, 344, 544
AvaII GGWCC 2 cut(s) 506, 678
BaeGI GKGCMC 1 cut(s) 904
BaeI ACNNNNGTAYC 2 cut(s) 778, 811
BanI GGYRCC 1 cut(s) 227
BbsI GAAGAC 2 cut(s) 718, 989
Bbv12I GWGCWC 2 cut(s) 408, 904
BbvI GCAGC 2 cut(s) 850, 905
BccI CCATC 4 cut(s) 182, 218, 406, 419
BceAI ACGGC 2 cut(s) 192, 373
BfaI CTAG 2 cut(s) 434, 612
BfmI CTRYAG 1 cut(s) 72
BfrI CTTAAG 2 cut(s) 161, 616
BisI GCNGC 2 cut(s) 864, 894
BlsI GCNGC 2 cut(s) 865, 895
Bme18I GGWCC 2 cut(s) 506, 678
BmgT120I GGNCC 3 cut(s) 140, 506, 678
BmiI GGNNCC 2 cut(s) 141, 229
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 325, 875
BmuI ACTGGG 1 cut(s) 160
BpiI GAAGAC 2 cut(s) 718, 989
BpmI CTGGAG 1 cut(s) 740
BpuEI CTTGAG 2 cut(s) 278, 861
BsaAI YACGTR 1 cut(s) 634
BsaJI CCNNGG 1 cut(s) 790
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 2 cut(s) 928, 929
Bse1I ACTGG 2 cut(s) 155, 723
Bse3DI GCAATG 1 cut(s) 770
BseDI CCNNGG 1 cut(s) 790
BseGI GGATG 2 cut(s) 229, 398
BseLI CCNNNNNNNGG 2 cut(s) 928, 929
BseMI GCAATG 1 cut(s) 770
BseNI ACTGG 2 cut(s) 155, 723
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 2 cut(s) 850, 905
Bsh1236I CGCG 2 cut(s) 317, 980
BshFI GGCC 3 cut(s) 142, 610, 769
BshNI GGYRCC 1 cut(s) 227
BsiHKAI GWGCWC 2 cut(s) 408, 904
BslFI GGGAC 1 cut(s) 927
BslI CCNNNNNNNGG 2 cut(s) 928, 929
BsmFI GGGAC 1 cut(s) 927
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 3 cut(s) 142, 610, 769
Bsp1286I GDGCHC 2 cut(s) 408, 904
Bsp143I GATC 6 cut(s) 240, 256, 341, 430, 638, 885
BspACI CCGC 1 cut(s) 968
BspANI GGCC 3 cut(s) 142, 610, 769
BspFNI CGCG 2 cut(s) 317, 980
BspLI GGNNCC 2 cut(s) 141, 229
BspPI GGATC 4 cut(s) 235, 264, 349, 646
BspT107I GGYRCC 1 cut(s) 227
BspTI CTTAAG 2 cut(s) 161, 616
BsrDI GCAATG 1 cut(s) 770
BsrI ACTGG 2 cut(s) 155, 723
BssECI CCNNGG 1 cut(s) 790
BssMI GATC 6 cut(s) 240, 256, 341, 430, 638, 885
Bst4CI ACNGT 5 cut(s) 473, 549, 682, 941, 955
BstAFI CTTAAG 2 cut(s) 161, 616
BstAPI GCANNNNNTGC 1 cut(s) 899
BstBAI YACGTR 1 cut(s) 634
BstC8I GCNNGC 1 cut(s) 181
BstEII GGTNACC 1 cut(s) 932
BstF5I GGATG 2 cut(s) 229, 398
BstFNI CGCG 2 cut(s) 317, 980
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 6 cut(s) 243, 259, 344, 433, 641, 888
BstMBI GATC 6 cut(s) 240, 256, 341, 430, 638, 885
BstMWI GCNNNNNNNGC 4 cut(s) 83, 274, 485, 899
BstNSI RCATGY 1 cut(s) 183
BstPI GGTNACC 1 cut(s) 932
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 904
BstUI CGCG 2 cut(s) 317, 980
BstV1I GCAGC 2 cut(s) 850, 905
BstV2I GAAGAC 2 cut(s) 718, 989
BstX2I RGATCY 2 cut(s) 256, 341
BstXI CCANNNNNNTGG 1 cut(s) 150
BstYI RGATCY 2 cut(s) 256, 341
BsuRI GGCC 3 cut(s) 142, 610, 769
BtsCI GGATG 2 cut(s) 229, 398
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 3 cut(s) 148, 478, 759
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 3 cut(s) 140, 506, 678
Csp6I GTAC 4 cut(s) 61, 449, 561, 660
CviAII CATG 3 cut(s) 180, 445, 819
CviQI GTAC 4 cut(s) 61, 449, 561, 660
DpnI GATC 6 cut(s) 242, 258, 343, 432, 640, 887
DpnII GATC 6 cut(s) 240, 256, 341, 430, 638, 885
Eco47I GGWCC 2 cut(s) 506, 678
Eco57I CTGAAG 1 cut(s) 684
Eco91I GGTNACC 1 cut(s) 932
EcoO65I GGTNACC 1 cut(s) 932
FaeI CATG 3 cut(s) 183, 448, 822
FaqI GGGAC 1 cut(s) 927
FatI CATG 3 cut(s) 179, 444, 818
Fnu4HI GCNGC 2 cut(s) 864, 894
FokI GGATG 2 cut(s) 236, 385
Fsp4HI GCNGC 2 cut(s) 864, 894
FspBI CTAG 2 cut(s) 434, 612
GlaI GCGC 1 cut(s) 318
GluI GCNGC 2 cut(s) 864, 894
GsuI CTGGAG 1 cut(s) 740
HaeIII GGCC 3 cut(s) 142, 610, 769
HhaI GCGC 1 cut(s) 319
Hin1II CATG 3 cut(s) 183, 448, 822
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HinfI GANTC 2 cut(s) 11, 881
HphI GGTGA 3 cut(s) 195, 344, 544
Hpy166II GTNNAC 3 cut(s) 451, 685, 902
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 4 cut(s) 295, 434, 708, 878
Hpy8I GTNNAC 3 cut(s) 451, 685, 902
HpyAV CCTTC 8 cut(s) 119, 130, 288, 296, 323, 499, 559, 683
HpyCH4III ACNGT 5 cut(s) 473, 549, 682, 941, 955
HpyCH4IV ACGT 1 cut(s) 633
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 274, 485, 899
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 3 cut(s) 183, 448, 822
HspAI GCGC 1 cut(s) 317
Kzo9I GATC 6 cut(s) 240, 256, 341, 430, 638, 885
LpnPI CCDG 6 cut(s) 136, 267, 324, 670, 704, 914
Lsp1109I GCAGC 2 cut(s) 850, 905
LweI GCATC 2 cut(s) 325, 875
MaeI CTAG 2 cut(s) 434, 612
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 3 cut(s) 305, 723, 932
MalI GATC 6 cut(s) 242, 258, 343, 432, 640, 887
MboI GATC 6 cut(s) 240, 256, 341, 430, 638, 885
MboII GAAGA 4 cut(s) 20, 47, 723, 989
MflI RGATCY 2 cut(s) 256, 341
MhlI GDGCHC 2 cut(s) 408, 904
MluCI AATT 5 cut(s) 48, 364, 437, 483, 1015
MlyI GAGTC 1 cut(s) 890
MnlI CCTC 7 cut(s) 214, 383, 503, 519, 663, 785, 885
MslI CAYNNNNRTG 2 cut(s) 236, 456
MspCI CTTAAG 2 cut(s) 161, 616
Mva1269I GAATGC 1 cut(s) 766
MvnI CGCG 2 cut(s) 317, 980
MwoI GCNNNNNNNGC 4 cut(s) 83, 274, 485, 899
NdeII GATC 6 cut(s) 240, 256, 341, 430, 638, 885
NlaIII CATG 3 cut(s) 183, 448, 822
NlaIV GGNNCC 2 cut(s) 141, 229
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 1 cut(s) 183
OliI CACNNNNGTG 2 cut(s) 236, 456
PacI TTAATTAA 1 cut(s) 1018
PaeI GCATGC 1 cut(s) 183
PctI GAATGC 1 cut(s) 766
PfeI GAWTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 865, 895
PleI GAGTC 1 cut(s) 889
PpsI GAGTC 1 cut(s) 889
Ppu21I YACGTR 1 cut(s) 634
PshBI ATTAAT 3 cut(s) 588, 858, 1014
PspEI GGTNACC 1 cut(s) 932
PspN4I GGNNCC 2 cut(s) 141, 229
PspPI GGNCC 3 cut(s) 140, 506, 678
PsuI RGATCY 2 cut(s) 256, 341
RsaI GTAC 4 cut(s) 62, 450, 562, 661
RsaNI GTAC 4 cut(s) 61, 449, 561, 660
RseI CAYNNNNRTG 2 cut(s) 236, 456
SatI GCNGC 2 cut(s) 864, 894
Sau3AI GATC 6 cut(s) 240, 256, 341, 430, 638, 885
Sau96I GGNCC 3 cut(s) 140, 506, 678
SchI GAGTC 1 cut(s) 890
SduI GDGCHC 2 cut(s) 408, 904
SfaNI GCATC 2 cut(s) 325, 875
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 2 cut(s) 506, 678
SmiMI CAYNNNNRTG 2 cut(s) 236, 456
SmlI CTYRAG 4 cut(s) 161, 293, 616, 876
SmoI CTYRAG 4 cut(s) 161, 293, 616, 876
SphI GCATGC 1 cut(s) 183
Sse9I AATT 5 cut(s) 48, 364, 437, 483, 1015
SsiI CCGC 1 cut(s) 968
SspMI CTAG 2 cut(s) 434, 612
TaaI ACNGT 5 cut(s) 473, 549, 682, 941, 955
TaiI ACGT 1 cut(s) 636
TaqI TCGA 2 cut(s) 812, 884
TaqII GACCGA 1 cut(s) 804
TasI AATT 5 cut(s) 48, 364, 437, 483, 1015
TatI WGTACW 2 cut(s) 60, 659
TfiI GAWTC 1 cut(s) 11
TscAI CASTG 3 cut(s) 155, 478, 759
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 2 cut(s) 863, 893
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 2 cut(s) 315, 773
TspRI CASTG 3 cut(s) 155, 478, 759
Vha464I CTTAAG 2 cut(s) 161, 616
VneI GTGCAC 1 cut(s) 900
VpaK11BI GGWCC 2 cut(s) 506, 678
VspI ATTAAT 3 cut(s) 588, 858, 1014
XbaI TCTAGA 1 cut(s) 433
XceI RCATGY 1 cut(s) 183
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XspI CTAG 2 cut(s) 434, 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.