Prupe.7G126300_v2.0.a1

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001343
Physical Location & Seq
Reverse (-)
14782505 .. 14783957
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G126300.1

Sequence Viewer

Length: 921 bp
ATGGTTGAATCAAAGCAAGCCCATCTTCAGTTCCTTGTATTTTCGTTATTTCTTCTCTACTGGCACCAAATACCAATTGGAGCCCAAGTTGTTGGTAATGCAAATATATTTACTTCTTGCACTCATCTCATTTACCAGCTTCATCATTATCACAGCCATTGGGTTTTTGGTGCAGGTCAAGAAATAGAGTTTGATTATAGGAATGGGAATGACAAGGGACCAGAACACTGGGGAGAGCTTAAGAAAGAATGGGCAGCATGCAAGTATGGAAAATTGCAATCACCAATAGATTTATCGGATGACCGTGCCACCAAAGTGATCTCAAGTTCAGAGGATCTTCAAATGAGTTACAAGCCCTGCAATGCAACCATGAAGAACGAAGGCCACTATATAGCGATGGAGTGGGAGGGTGATGCTGGATCAGTCAAAATCAATGGCACAGATTACTTCCTCAAGCAATGCCATTGGCACACCCCCTCTGAGCATTCCATTAATGGCATCAGGTATGATTTGGAGCTGCACATGCTTCACCGAAGTCCGGACCTGAACAATAAAGCTGTAGTTGCACTTCTATTCAAAATTGGTAGCCCCAATCCTCTTCTCTCAAAGGTGCACAACGGCTTAGAGTCTATGATTGGTACAAAGGAGACACAACTGGAACTGACTGATCCAAGTGAGATAAAATGGAGCAGCTTAAAATTTTACAGATACATTGGCTCACTCACAACCCCTCCTTGCACTGAAGGAGTTATTTGGATCGTAAACGAAATGGTAAATACTGTTTCGAAAGAACAACTGGAGTTACTTAAACAAGCTGTTTATGATTATGCAAAGATGAACGCAAGACCATCACAGCCTCTCAACGACCGAGACATCATGCTCTACAGCACGTTGTTAAGACAGCCAGTGTTGCGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

307

Amino Acids

35.08

Weight (kDa)

6.3

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 63
AccIII TCCGGA 1 cut(s) 538
AclWI GGATC 4 cut(s) 342, 427, 662, 764
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 2 cut(s) 11, 762
AfaI GTAC 1 cut(s) 640
AfiI CCNNNNNNNGG 1 cut(s) 538
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 3 cut(s) 8, 341, 577
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 6 cut(s) 139, 238, 517, 557, 693, 815
AluI AGCT 6 cut(s) 139, 238, 517, 557, 693, 815
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 2 cut(s) 641, 864
Alw44I GTGCAC 1 cut(s) 611
AlwI GGATC 4 cut(s) 342, 427, 662, 764
Aor13HI TCCGGA 1 cut(s) 538
AoxI GGCC 1 cut(s) 382
ApaLI GTGCAC 1 cut(s) 611
ApeKI GCWGC 3 cut(s) 254, 517, 690
ApoI RAATTY 1 cut(s) 698
AseI ATTAAT 1 cut(s) 492
AspS9I GGNCC 2 cut(s) 218, 541
AsuHPI GGTGA 3 cut(s) 273, 422, 521
AsuII TTCGAA 1 cut(s) 785
AvaII GGWCC 2 cut(s) 218, 541
BaeGI GKGCMC 1 cut(s) 615
BanI GGYRCC 1 cut(s) 63
BanII GRGCYC 1 cut(s) 85
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 3 cut(s) 266, 504, 702
BccI CCATC 3 cut(s) 30, 391, 856
BceAI ACGGC 1 cut(s) 634
BcoDI GTCTC 2 cut(s) 641, 864
BfaI CTAG 1 cut(s) 919
BfmI CTRYAG 2 cut(s) 558, 883
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 1 cut(s) 164
BisI GCNGC 3 cut(s) 255, 518, 691
BlsI GCNGC 3 cut(s) 256, 519, 692
Bme18I GGWCC 2 cut(s) 218, 541
BmgT120I GGNCC 2 cut(s) 218, 541
BmiI GGNNCC 3 cut(s) 65, 82, 219
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 403, 507
BmuI ACTGGG 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 818
Bpu14I TTCGAA 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 307, 437
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 4 cut(s) 72, 102, 715, 745
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse1I ACTGG 5 cut(s) 65, 233, 660, 801, 905
Bse3DI GCAATG 2 cut(s) 367, 464
BseAI TCCGGA 1 cut(s) 538
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 2 cut(s) 367, 464
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 5 cut(s) 65, 233, 660, 801, 905
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 3 cut(s) 266, 504, 702
BsgI GTGCAG 2 cut(s) 192, 503
Bsh1285I CGRYCG 1 cut(s) 868
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 868
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 1 cut(s) 539
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 2 cut(s) 641, 864
BsmFI GGGAC 1 cut(s) 231
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 1 cut(s) 384
Bsp119I TTCGAA 1 cut(s) 785
Bsp1286I GDGCHC 2 cut(s) 85, 615
Bsp13I TCCGGA 1 cut(s) 538
Bsp143I GATC 5 cut(s) 318, 334, 419, 667, 756
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 1 cut(s) 472
BspEI TCCGGA 1 cut(s) 538
BspLI GGNNCC 3 cut(s) 65, 82, 219
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 4 cut(s) 342, 427, 662, 764
BspT104I TTCGAA 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 63
BspTI CTTAAG 1 cut(s) 239
BsrDI GCAATG 2 cut(s) 367, 464
BsrI ACTGG 5 cut(s) 65, 233, 660, 801, 905
BssMI GATC 5 cut(s) 318, 334, 419, 667, 756
Bst4CI ACNGT 2 cut(s) 305, 781
Bst6I CTCTTC 1 cut(s) 603
BstAFI CTTAAG 1 cut(s) 239
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 2 cut(s) 18, 259
BstDEI CTNAG 2 cut(s) 480, 622
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 5 cut(s) 321, 337, 422, 670, 759
BstMAI GTCTC 2 cut(s) 641, 864
BstMBI GATC 5 cut(s) 318, 334, 419, 667, 756
BstMCI CGRYCG 1 cut(s) 868
BstMWI GCNNNNNNNGC 3 cut(s) 523, 563, 910
BstNSI RCATGY 2 cut(s) 261, 526
BstSFI CTRYAG 2 cut(s) 558, 883
BstSLI GKGCMC 1 cut(s) 615
BstV1I GCAGC 3 cut(s) 266, 504, 702
BstX2I RGATCY 1 cut(s) 334
BstXI CCANNNNNNTGG 2 cut(s) 92, 228
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 410
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 3 cut(s) 226, 738, 912
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 18, 259
Cfr13I GGNCC 2 cut(s) 218, 541
Csp6I GTAC 1 cut(s) 639
CviAII CATG 4 cut(s) 258, 370, 523, 877
CviQI GTAC 1 cut(s) 639
DdeI CTNAG 2 cut(s) 480, 622
DpnI GATC 5 cut(s) 320, 336, 421, 669, 758
DpnII GATC 5 cut(s) 318, 334, 419, 667, 756
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco24I GRGCYC 1 cut(s) 85
Eco47I GGWCC 2 cut(s) 218, 541
Eco57I CTGAAG 2 cut(s) 11, 762
EcoT38I GRGCYC 1 cut(s) 85
FaeI CATG 4 cut(s) 261, 373, 526, 880
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 231
FatI CATG 4 cut(s) 257, 369, 522, 876
Fnu4HI GCNGC 3 cut(s) 255, 518, 691
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 3 cut(s) 255, 518, 691
FspBI CTAG 1 cut(s) 919
GluI GCNGC 3 cut(s) 255, 518, 691
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 539
Hin1II CATG 4 cut(s) 261, 373, 526, 880
HinfI GANTC 2 cut(s) 8, 626
HpaII CCGG 1 cut(s) 539
HphI GGTGA 3 cut(s) 273, 422, 521
Hpy166II GTNNAC 2 cut(s) 613, 763
Hpy188I TCNGA 3 cut(s) 298, 331, 481
Hpy188III TCNNGA 2 cut(s) 179, 539
Hpy8I GTNNAC 2 cut(s) 613, 763
HpyAV CCTTC 2 cut(s) 374, 737
HpyCH4III ACNGT 2 cut(s) 305, 781
HpyCH4IV ACGT 1 cut(s) 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 523, 563, 910
HpyF3I CTNAG 2 cut(s) 480, 622
HpySE526I ACGT 1 cut(s) 890
Hsp92II CATG 4 cut(s) 261, 373, 526, 880
Kpn2I TCCGGA 1 cut(s) 538
Kzo9I GATC 5 cut(s) 318, 334, 419, 667, 756
LmnI GCTCC 3 cut(s) 80, 514, 687
Lsp1109I GCAGC 3 cut(s) 266, 504, 702
LweI GCATC 2 cut(s) 403, 507
MaeI CTAG 1 cut(s) 919
MaeII ACGT 1 cut(s) 890
MaeIII GTNAC 2 cut(s) 347, 801
MalI GATC 5 cut(s) 320, 336, 421, 669, 758
MboI GATC 5 cut(s) 318, 334, 419, 667, 756
MboII GAAGA 5 cut(s) 17, 44, 329, 385, 590
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 85, 615
MluCI AATT 4 cut(s) 75, 272, 579, 698
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 7 cut(s) 325, 400, 461, 487, 606, 741, 867
MroI TCCGGA 1 cut(s) 538
MseI TTAA 5 cut(s) 240, 492, 695, 807, 896
MslI CAYNNNNRTG 1 cut(s) 314
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 1 cut(s) 539
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 484
MwoI GCNNNNNNNGC 3 cut(s) 523, 563, 910
NdeII GATC 5 cut(s) 318, 334, 419, 667, 756
NlaIII CATG 4 cut(s) 261, 373, 526, 880
NlaIV GGNNCC 3 cut(s) 65, 82, 219
NspI RCATGY 2 cut(s) 261, 526
NspV TTCGAA 1 cut(s) 785
OliI CACNNNNGTG 1 cut(s) 314
PaeI GCATGC 1 cut(s) 261
PctI GAATGC 1 cut(s) 484
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 3 cut(s) 256, 519, 692
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
PshBI ATTAAT 1 cut(s) 492
PspN4I GGNNCC 3 cut(s) 65, 82, 219
PspPI GGNCC 2 cut(s) 218, 541
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 640
RsaNI GTAC 1 cut(s) 639
RseI CAYNNNNRTG 1 cut(s) 314
SaqAI TTAA 5 cut(s) 240, 492, 695, 807, 896
SatI GCNGC 3 cut(s) 255, 518, 691
Sau3AI GATC 5 cut(s) 318, 334, 419, 667, 756
Sau96I GGNCC 2 cut(s) 218, 541
SchI GAGTC 1 cut(s) 635
SduI GDGCHC 2 cut(s) 85, 615
SfaNI GCATC 2 cut(s) 403, 507
SfcI CTRYAG 2 cut(s) 558, 883
SfuI TTCGAA 1 cut(s) 785
SinI GGWCC 2 cut(s) 218, 541
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 3 cut(s) 239, 322, 452
SmoI CTYRAG 3 cut(s) 239, 322, 452
SphI GCATGC 1 cut(s) 261
Sse9I AATT 4 cut(s) 75, 272, 579, 698
SspMI CTAG 1 cut(s) 919
TaaI ACNGT 2 cut(s) 305, 781
TaiI ACGT 1 cut(s) 893
TaqI TCGA 1 cut(s) 785
TaqII GACCGA 1 cut(s) 882
TasI AATT 4 cut(s) 75, 272, 579, 698
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 5 cut(s) 240, 492, 695, 807, 896
Tru9I TTAA 5 cut(s) 240, 492, 695, 807, 896
TscAI CASTG 3 cut(s) 233, 745, 912
TseI GCWGC 3 cut(s) 254, 517, 690
TspDTI ATGAA 3 cut(s) 131, 386, 851
TspRI CASTG 3 cut(s) 233, 745, 912
Vha464I CTTAAG 1 cut(s) 239
VneI GTGCAC 1 cut(s) 611
VpaK11BI GGWCC 2 cut(s) 218, 541
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 1 cut(s) 698
XceI RCATGY 2 cut(s) 261, 526
XcmI CCANNNNNNNNNTGG 2 cut(s) 74, 164
XspI CTAG 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.