RLG00000024240

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001343
Physical Location & Seq
Forward (+)
33403028 .. 33404430
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024240

Sequence Viewer

Length: 804 bp
ATGGTTAAATCAAAGAAGGTCCATCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACACCATCAGACCCGTCAGAGGCCATGGAGTGGAAGAAATAGGGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAAAGCACTGGGGAGAGCTCAAGGAAGAATGGAAGGAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTCGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAAGCCTTCAAATGCAACTATGAAGCATGATGGTTATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGATTACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGACGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTTTACCAAAGTGGCAATGCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCCTGAGTGGTACAAAGGAGGCACGCTTGGGAGTGATTGATCCAAGTTTAATAAAATGGCCAAGCTCACGATTTTACAGATATATGGGCTCACTGACGACCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTATACGAAGAGGTACATTCTGTTTCAAAAGAACAGCTGCGGCTAATTGAACAAGTCGATCATGGGATGAATGCAAGACCATTACAGCCTCTTAACAATCGAGAAATCACGTTCTTTGGTCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

268

Amino Acids

30.61

Weight (kDa)

7.78

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 262 2.5e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 94
AccI GTMKAC 1 cut(s) 678
AciI CCGC 2 cut(s) 715, 797
AclWI GGATC 4 cut(s) 346, 387, 503, 578
AcoI YGGCCR 1 cut(s) 602
AcuI CTGAAG 2 cut(s) 11, 678
AfaI GTAC 2 cut(s) 556, 690
AfiI CCNNNNNNNGG 2 cut(s) 83, 94
AgsI TTSAA 5 cut(s) 279, 320, 347, 702, 725
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 4 cut(s) 157, 267, 609, 712
AluI AGCT 4 cut(s) 157, 267, 609, 712
Alw21I GWGCWC 1 cut(s) 159
AlwI GGATC 4 cut(s) 346, 387, 503, 578
AoxI GGCC 2 cut(s) 85, 602
ApeKI GCWGC 1 cut(s) 712
AseI ATTAAT 1 cut(s) 411
AspS9I GGNCC 3 cut(s) 19, 137, 794
AsuHPI GGTGA 2 cut(s) 341, 440
AsuII TTCGAA 1 cut(s) 221
AvaII GGWCC 3 cut(s) 19, 137, 794
BalI TGGCCA 1 cut(s) 604
BanII GRGCYC 3 cut(s) 131, 159, 635
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 699
BccI CCATC 4 cut(s) 30, 77, 179, 293
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 550
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 474
BfuI GTATCC 1 cut(s) 550
BisI GCNGC 2 cut(s) 713, 716
BlsI GCNGC 2 cut(s) 714, 717
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 3 cut(s) 19, 137, 794
BmgT120I GGNCC 3 cut(s) 19, 137, 794
BmiI GGNNCC 2 cut(s) 128, 138
BmrFI CCNGG 1 cut(s) 61
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 157
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 2 cut(s) 143, 226
BsaJI CCNNGG 2 cut(s) 88, 132
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 94
Bse1I ACTGG 1 cut(s) 152
Bse3DI GCAATG 1 cut(s) 508
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 88, 132
BseGI GGATG 1 cut(s) 747
BseLI CCNNNNNNNGG 2 cut(s) 83, 94
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 699
BshFI GGCC 2 cut(s) 87, 604
BsiHKAI GWGCWC 1 cut(s) 159
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 2 cut(s) 83, 94
BsmFI GGGAC 1 cut(s) 150
BsmI GAATGC 4 cut(s) 182, 229, 482, 751
BsnI GGCC 2 cut(s) 87, 604
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 3 cut(s) 131, 159, 635
Bsp143I GATC 6 cut(s) 253, 338, 392, 508, 583, 733
Bsp19I CCATGG 1 cut(s) 88
BspACI CCGC 2 cut(s) 715, 797
BspANI GGCC 2 cut(s) 87, 604
BspCNI CTCAG 1 cut(s) 540
BspLI GGNNCC 2 cut(s) 128, 138
BspPI GGATC 4 cut(s) 346, 387, 503, 578
BspT104I TTCGAA 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 88, 132
BssMI GATC 6 cut(s) 253, 338, 392, 508, 583, 733
BssNAI GTATAC 1 cut(s) 679
BssT1I CCWWGG 2 cut(s) 88, 132
Bst1107I GTATAC 1 cut(s) 679
Bst2UI CCWGG 1 cut(s) 61
Bst6I CTCTTC 1 cut(s) 678
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 568
BstDEI CTNAG 1 cut(s) 548
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 1 cut(s) 747
BstKTI GATC 6 cut(s) 256, 341, 395, 511, 586, 736
BstMBI GATC 6 cut(s) 253, 338, 392, 508, 583, 733
BstMWI GCNNNNNNNGC 2 cut(s) 233, 479
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 699
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 147
BstYI RGATCY 1 cut(s) 392
BstZ17I GTATAC 1 cut(s) 679
BsuI GTATCC 1 cut(s) 550
BsuRI GGCC 2 cut(s) 87, 604
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 1 cut(s) 747
BtsIMutI CAGTG 4 cut(s) 145, 357, 635, 654
Cac8I GCNNGC 1 cut(s) 568
Cfr13I GGNCC 3 cut(s) 19, 137, 794
Csp6I GTAC 2 cut(s) 555, 689
CviAII CATG 5 cut(s) 89, 262, 296, 442, 737
CviQI GTAC 2 cut(s) 555, 689
DdeI CTNAG 1 cut(s) 548
DpnI GATC 6 cut(s) 255, 340, 394, 510, 585, 735
DpnII GATC 6 cut(s) 253, 338, 392, 508, 583, 733
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 88, 132
Eco24I GRGCYC 3 cut(s) 131, 159, 635
Eco47I GGWCC 3 cut(s) 19, 137, 794
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 11, 678
EcoICRI GAGCTC 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 88, 132
EcoT38I GRGCYC 3 cut(s) 131, 159, 635
ErhI CCWWGG 2 cut(s) 88, 132
FaeI CATG 5 cut(s) 92, 265, 299, 445, 740
FaqI GGGAC 1 cut(s) 150
FatI CATG 5 cut(s) 88, 261, 295, 441, 736
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 2 cut(s) 713, 716
FokI GGATG 1 cut(s) 754
FriOI GRGCYC 3 cut(s) 131, 159, 635
Fsp4HI GCNGC 2 cut(s) 713, 716
FspBI CTAG 1 cut(s) 228
GluI GCNGC 2 cut(s) 713, 716
HaeIII GGCC 2 cut(s) 87, 604
Hin1II CATG 5 cut(s) 92, 265, 299, 445, 740
HinfI GANTC 2 cut(s) 218, 316
HphI GGTGA 2 cut(s) 341, 440
Hpy166II GTNNAC 2 cut(s) 448, 679
Hpy188I TCNGA 3 cut(s) 74, 83, 400
Hpy188III TCNNGA 3 cut(s) 243, 612, 776
Hpy8I GTNNAC 2 cut(s) 448, 679
HpyAV CCTTC 4 cut(s) 10, 166, 285, 653
HpyCH4IV ACGT 2 cut(s) 421, 785
HpyCH4V TGCA 5 cut(s) 180, 284, 439, 654, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 479
HpyF3I CTNAG 1 cut(s) 548
HpySE526I ACGT 2 cut(s) 421, 785
Hsp92II CATG 5 cut(s) 92, 265, 299, 445, 740
Kzo9I GATC 6 cut(s) 253, 338, 392, 508, 583, 733
LmnI GCTCC 2 cut(s) 126, 672
LpnPI CCDG 7 cut(s) 46, 73, 133, 321, 470, 530, 560
Lsp1109I GCAGC 1 cut(s) 699
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 228
MaeII ACGT 2 cut(s) 421, 785
MalI GATC 6 cut(s) 255, 340, 394, 510, 585, 735
MboI GATC 6 cut(s) 253, 338, 392, 508, 583, 733
MboII GAAGA 5 cut(s) 17, 44, 110, 176, 695
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 3 cut(s) 131, 159, 635
MlsI TGGCCA 1 cut(s) 604
MluCI AATT 4 cut(s) 191, 342, 434, 720
MluNI TGGCCA 1 cut(s) 604
MnlI CCTC 8 cut(s) 77, 319, 406, 553, 556, 657, 679, 774
Mox20I TGGCCA 1 cut(s) 604
MscI TGGCCA 1 cut(s) 604
MseI TTAA 5 cut(s) 6, 258, 411, 593, 768
Msp20I TGGCCA 1 cut(s) 604
MspA1I CMGCKG 1 cut(s) 712
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 4 cut(s) 182, 229, 482, 751
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 233, 479
NcoI CCATGG 1 cut(s) 88
NdeII GATC 6 cut(s) 253, 338, 392, 508, 583, 733
NlaIII CATG 5 cut(s) 92, 265, 299, 445, 740
NlaIV GGNNCC 2 cut(s) 128, 138
NspV TTCGAA 1 cut(s) 221
PctI GAATGC 4 cut(s) 182, 229, 482, 751
PfeI GAWTC 2 cut(s) 218, 316
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 2 cut(s) 714, 717
PshBI ATTAAT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 128, 138
PspPI GGNCC 3 cut(s) 19, 137, 794
PsuI RGATCY 1 cut(s) 392
PvuII CAGCTG 1 cut(s) 712
RsaI GTAC 2 cut(s) 556, 690
RsaNI GTAC 2 cut(s) 555, 689
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 5 cut(s) 6, 258, 411, 593, 768
SatI GCNGC 2 cut(s) 713, 716
Sau3AI GATC 6 cut(s) 253, 338, 392, 508, 583, 733
Sau96I GGNCC 3 cut(s) 19, 137, 794
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 3 cut(s) 131, 159, 635
SetI ASST 8 cut(s) 21, 159, 269, 424, 611, 690, 714, 788
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 3 cut(s) 19, 137, 794
SmlI CTYRAG 2 cut(s) 158, 241
SmoI CTYRAG 2 cut(s) 158, 241
Sse9I AATT 4 cut(s) 191, 342, 434, 720
SsiI CCGC 2 cut(s) 715, 797
SspMI CTAG 1 cut(s) 228
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 2 cut(s) 88, 132
TaiI ACGT 2 cut(s) 424, 788
TaqI TCGA 5 cut(s) 216, 221, 314, 732, 775
TasI AATT 4 cut(s) 191, 342, 434, 720
TauI GCSGC 1 cut(s) 718
TfiI GAWTC 2 cut(s) 218, 316
Tru1I TTAA 5 cut(s) 6, 258, 411, 593, 768
Tru9I TTAA 5 cut(s) 6, 258, 411, 593, 768
TscAI CASTG 4 cut(s) 152, 364, 642, 661
TseI GCWGC 1 cut(s) 712
TspDTI ATGAA 3 cut(s) 305, 397, 758
TspRI CASTG 4 cut(s) 152, 364, 642, 661
Van91I CCANNNNNTGG 1 cut(s) 94
VpaK11BI GGWCC 3 cut(s) 19, 137, 794
VspI ATTAAT 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 678
XspI CTAG 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.