Rh3DG189000

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001343
Physical Location & Seq
Reverse (-)
16847092 .. 16851037
3946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG189000.1

Sequence Viewer

Length: 870 bp
ATGTTTGTATCAAAGGAATCCCTTCTTGGCTTCATTGTTTTTACCTTTTTTCTTCTTTGCTGGAACTCGACACCCATCAGAGCAAACGACGATGGGGAAGTAGACTTTGATTATCGGGAAGACCACCCGAGAGGACCACAACACTGGGGATACCTTAAGAAAGAATGGAGCTTATGCAAGGATGGAAAAAGGCAATCACCAATAGAGATATGGGAAACGAATGCCACCAAAGACATCACAGATTCAAAGGATCTTATAGTGAGTTATAAGCCTGCAAATGCAACTATCAAAAATGAAGAACATGCTATATCGGTTTTATGGGAGGGTGATGCTGGCTCAATTGAAATCAATGGCACAACCTACTTCCTCAAACAATGTCATTGGCACCACCCCTCTGAGCATGTCGTTAATGGCAGAAGATTTATCTTTATTAGGTTTGATCTGGAATTGCACATGGTTCACAAAACTCAGGACATGAACAAAATAGCTGTAATCGGATTCCTCTACAACTTTGGTGCACCTAATTCTTTTCTCACAAAGGTGACAGGACACATCACTTCTTTGACTGATGTACAAAAGGGGATACCCTTGGGAGTGCTTGATCCAAGGAAGGTGAAAAAGGGTCAACTCCATTACAAGATTGCGAAGCCAGAAAAGGCTGGCTCTAAATTTTACAGATACATGGGCTCTCTCACAACCCCTCCTTGTGCTGAAGAGGTTATTTGGCACATAAACAAAAAGGTACAAACTGCTTCGATAGAACAAGTGAAGCTACTCCAAGATGCAGTTTTTGATTTTGCGGCGAACAACGCAAGACCATTACAACCTCGCAACGGCCGAGGAATCAAGCTCTATGGGGACTATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

289

Amino Acids

33.12

Weight (kDa)

8.71

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 283 1.2e-34 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 102
AciI CCGC 1 cut(s) 800
AclWI GGATC 2 cut(s) 258, 596
AcoI YGGCCR 1 cut(s) 835
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 2 cut(s) 573, 744
AfiI CCNNNNNNNGG 1 cut(s) 833
AflII CTTAAG 1 cut(s) 155
AgsI TTSAA 2 cut(s) 246, 344
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AleI CACNNNNGTG 1 cut(s) 539
AluBI AGCT 4 cut(s) 171, 488, 772, 850
AluI AGCT 4 cut(s) 171, 488, 772, 850
Alw21I GWGCWC 1 cut(s) 520
Alw44I GTGCAC 1 cut(s) 516
AlwI GGATC 2 cut(s) 258, 596
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 1 cut(s) 835
ApaLI GTGCAC 1 cut(s) 516
ApoI RAATTY 1 cut(s) 668
Asp700I GAANNNNTTC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 4 cut(s) 189, 338, 553, 625
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 520
BanI GGYRCC 1 cut(s) 384
BanII GRGCYC 1 cut(s) 689
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 520
BccI CCATC 3 cut(s) 83, 86, 176
BceAI ACGGC 1 cut(s) 850
BciVI GTATCC 2 cut(s) 143, 576
BfrI CTTAAG 1 cut(s) 155
BfuI GTATCC 2 cut(s) 143, 576
BisI GCNGC 1 cut(s) 801
BlsI GCNGC 1 cut(s) 802
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 386
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 319, 772
BmuI ACTGGG 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 588, 605, 838
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 833
Bse1I ACTGG 1 cut(s) 149
BseDI CCNNGG 3 cut(s) 588, 605, 838
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 833
BseMII CTCAG 2 cut(s) 387, 482
BseNI ACTGG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 520
BseX3I CGGCCG 1 cut(s) 835
Bsh1285I CGRYCG 1 cut(s) 838
BshFI GGCC 1 cut(s) 837
BshNI GGYRCC 1 cut(s) 384
BsiEI CGRYCG 1 cut(s) 838
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 833
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 1 cut(s) 837
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 520, 689
Bsp1407I TGTACA 1 cut(s) 571
Bsp143I GATC 3 cut(s) 250, 439, 601
BspACI CCGC 1 cut(s) 800
BspANI GGCC 1 cut(s) 837
BspCNI CTCAG 2 cut(s) 388, 481
BspLI GGNNCC 1 cut(s) 386
BspPI GGATC 2 cut(s) 258, 596
BspT107I GGYRCC 1 cut(s) 384
BspTI CTTAAG 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 571
BsrI ACTGG 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 588, 605, 838
BssMI GATC 3 cut(s) 250, 439, 601
BssT1I CCWWGG 2 cut(s) 588, 605
Bst6I CTCTTC 1 cut(s) 708
BstAFI CTTAAG 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 571
BstC8I GCNNGC 3 cut(s) 273, 334, 661
BstDEI CTNAG 2 cut(s) 396, 468
BstF5I GGATG 1 cut(s) 187
BstKTI GATC 3 cut(s) 253, 442, 604
BstMBI GATC 3 cut(s) 250, 439, 601
BstMCI CGRYCG 1 cut(s) 838
BstMWI GCNNNNNNNGC 1 cut(s) 809
BstNSI RCATGY 2 cut(s) 305, 404
BstSLI GKGCMC 1 cut(s) 520
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 250
BstXI CCANNNNNNTGG 1 cut(s) 144
BstYI RGATCY 1 cut(s) 250
BstZI CGGCCG 1 cut(s) 835
BsuI GTATCC 2 cut(s) 143, 576
BsuRI GGCC 1 cut(s) 837
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 3 cut(s) 273, 334, 661
Cfr13I GGNCC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 572, 743
CviAII CATG 5 cut(s) 302, 401, 454, 475, 682
CviQI GTAC 2 cut(s) 572, 743
DdeI CTNAG 2 cut(s) 396, 468
DpnI GATC 3 cut(s) 252, 441, 603
DpnII GATC 3 cut(s) 250, 439, 601
EaeI YGGCCR 1 cut(s) 835
EagI CGGCCG 1 cut(s) 835
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
EclXI CGGCCG 1 cut(s) 835
Eco130I CCWWGG 2 cut(s) 588, 605
Eco24I GRGCYC 1 cut(s) 689
Eco47I GGWCC 1 cut(s) 134
Eco52I CGGCCG 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 732
Eco88I CYCGRG 1 cut(s) 127
EcoT14I CCWWGG 2 cut(s) 588, 605
EcoT38I GRGCYC 1 cut(s) 689
ErhI CCWWGG 2 cut(s) 588, 605
FaeI CATG 5 cut(s) 305, 404, 457, 478, 685
FatI CATG 5 cut(s) 301, 400, 453, 474, 681
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 801
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 689
Fsp4HI GCNGC 1 cut(s) 801
GluI GCNGC 1 cut(s) 801
HaeIII GGCC 1 cut(s) 837
Hin1II CATG 5 cut(s) 305, 404, 457, 478, 685
HincII GTYRAC 1 cut(s) 626
HindII GTYRAC 1 cut(s) 626
HinfI GANTC 4 cut(s) 17, 242, 498, 843
HphI GGTGA 4 cut(s) 189, 338, 553, 625
Hpy166II GTNNAC 4 cut(s) 103, 460, 518, 626
Hpy188I TCNGA 3 cut(s) 80, 397, 497
Hpy188III TCNNGA 3 cut(s) 116, 443, 470
Hpy8I GTNNAC 4 cut(s) 103, 460, 518, 626
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 2 cut(s) 32, 604
HpyCH4V TGCA 6 cut(s) 177, 275, 281, 451, 518, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 809
HpyF3I CTNAG 2 cut(s) 396, 468
Hsp92II CATG 5 cut(s) 305, 404, 457, 478, 685
Kzo9I GATC 3 cut(s) 250, 439, 601
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 9 cut(s) 46, 130, 285, 318, 428, 455, 531, 645, 663
LweI GCATC 2 cut(s) 319, 772
MaeIII GTNAC 1 cut(s) 541
MalI GATC 3 cut(s) 252, 441, 603
MboI GATC 3 cut(s) 250, 439, 601
MboII GAAGA 5 cut(s) 44, 131, 308, 429, 725
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 520, 689
MluCI AATT 4 cut(s) 339, 446, 523, 668
MnlI CCTC 9 cut(s) 125, 316, 377, 403, 512, 709, 711, 833, 837
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 2 cut(s) 156, 408
MslI CAYNNNNRTG 1 cut(s) 539
MspCI CTTAAG 1 cut(s) 155
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 809
NdeII GATC 3 cut(s) 250, 439, 601
NlaIII CATG 5 cut(s) 305, 404, 457, 478, 685
NlaIV GGNNCC 1 cut(s) 386
NmeAIII GCCGAG 1 cut(s) 863
NmuCI GTSAC 1 cut(s) 541
NspI RCATGY 2 cut(s) 305, 404
OliI CACNNNNGTG 1 cut(s) 539
PcsI WCGNNNNNNNCGW 1 cut(s) 835
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 4 cut(s) 17, 242, 498, 843
PkrI GCNGC 1 cut(s) 802
PsiI TTATAA 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 386
PspPI GGNCC 1 cut(s) 134
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 2 cut(s) 573, 744
RsaNI GTAC 2 cut(s) 572, 743
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 2 cut(s) 156, 408
SatI GCNGC 1 cut(s) 801
Sau3AI GATC 3 cut(s) 250, 439, 601
Sau96I GGNCC 1 cut(s) 134
SduI GDGCHC 2 cut(s) 520, 689
SfaNI GCATC 2 cut(s) 319, 772
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 4 cut(s) 339, 446, 523, 668
SsiI CCGC 1 cut(s) 800
StyI CCWWGG 2 cut(s) 588, 605
TaqI TCGA 2 cut(s) 68, 755
TasI AATT 4 cut(s) 339, 446, 523, 668
TatI WGTACW 1 cut(s) 571
TauI GCSGC 1 cut(s) 803
TfiI GAWTC 4 cut(s) 17, 242, 498, 843
Tru1I TTAA 2 cut(s) 156, 408
Tru9I TTAA 2 cut(s) 156, 408
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 1 cut(s) 541
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 3 cut(s) 22, 309, 491
TspRI CASTG 1 cut(s) 149
Vha464I CTTAAG 1 cut(s) 155
VneI GTGCAC 1 cut(s) 516
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 1 cut(s) 668
XceI RCATGY 2 cut(s) 305, 404
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.