Rroxscaffold_6G00410480

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001343
Physical Location & Seq
Forward (+)
32912775 .. 32915105
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410480.1

Sequence Viewer

Length: 819 bp
ATGATTAACGTAGTGATGGTTAAATCAAAGAAATTCCATCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACCCCATCAGACCCGTCAGAGGCCAAGGAGTGGAAGAAATAGGGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAAGGAAGAATGGAAGGAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTGGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAGCATGAGCTATAAGCCTTCAAATGCAACTATGAAGCATGAAGGTTATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGATTACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGCTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCTCATTCCTATACCGAAGTGGCAATCCCGATCCTTTCTTGTCAAAGATAAGCAACGATGTATCCTCTCTAAGTGGTACAAAGGAGGCACGCTTGGGAGTGATTGATCCTAGTTTAATAAAATGGCCAAGCTCACGATTTTACAGATATATGGGCTCACTCACGACCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTATACGAAGAGGTACATTCTGTTTCAAAAGAACAGCTGAGGCTAATTGAACAAGTCGATCATGGGATGAATGCAAGACCATTACAGCCTCTTAACAATAGAGATATCACGCTCTTTGGTCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

272

Amino Acids

31.11

Weight (kDa)

6.83

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 50 - 267 8.2e-37 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 78
AccB7I CCANNNNNTGG 1 cut(s) 109
AccI GTMKAC 1 cut(s) 693
AciI CCGC 1 cut(s) 812
AclWI GGATC 4 cut(s) 361, 402, 518, 593
AcoI YGGCCR 1 cut(s) 617
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 2 cut(s) 26, 693
AfaI GTAC 2 cut(s) 571, 705
AfiI CCNNNNNNNGG 2 cut(s) 98, 109
AgsI TTSAA 5 cut(s) 294, 335, 362, 717, 740
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 5 cut(s) 172, 282, 438, 624, 727
AluI AGCT 5 cut(s) 172, 282, 438, 624, 727
Alw21I GWGCWC 2 cut(s) 162, 174
Alw26I GTCTC 1 cut(s) 499
AlwI GGATC 4 cut(s) 361, 402, 518, 593
AoxI GGCC 2 cut(s) 100, 617
ApoI RAATTY 1 cut(s) 32
AseI ATTAAT 1 cut(s) 426
AspS9I GGNCC 2 cut(s) 152, 809
AsuHPI GGTGA 2 cut(s) 356, 455
AsuII TTCGAA 1 cut(s) 236
AvaII GGWCC 2 cut(s) 152, 809
BalI TGGCCA 1 cut(s) 619
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 3 cut(s) 146, 174, 650
Bbv12I GWGCWC 2 cut(s) 162, 174
BbvCI CCTCAGC 1 cut(s) 728
BccI CCATC 4 cut(s) 10, 45, 92, 194
BciT130I CCWGG 1 cut(s) 76
BciVI GTATCC 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 499
BfaI CTAG 2 cut(s) 243, 603
BfmI CTRYAG 1 cut(s) 489
BfuI GTATCC 1 cut(s) 565
Bme1390I CCNGG 1 cut(s) 76
Bme18I GGWCC 2 cut(s) 152, 809
BmgT120I GGNCC 2 cut(s) 152, 809
BmiI GGNNCC 3 cut(s) 80, 143, 153
BmrFI CCNGG 1 cut(s) 76
BmrI ACTGGG 1 cut(s) 172
BmsI GCATC 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 728
Bpu14I TTCGAA 1 cut(s) 236
BpuEI CTTGAG 2 cut(s) 158, 241
BsaJI CCNNGG 2 cut(s) 103, 147
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 109
Bse1I ACTGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 76
BseDI CCNNGG 2 cut(s) 103, 147
BseGI GGATG 1 cut(s) 762
BseLI CCNNNNNNNGG 2 cut(s) 98, 109
BseMII CTCAG 1 cut(s) 719
BseNI ACTGG 1 cut(s) 167
BshFI GGCC 2 cut(s) 102, 619
BshNI GGYRCC 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 162, 174
BslFI GGGAC 1 cut(s) 165
BslI CCNNNNNNNGG 2 cut(s) 98, 109
BsmAI GTCTC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 165
BsmI GAATGC 3 cut(s) 197, 244, 766
BsnI GGCC 2 cut(s) 102, 619
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 4 cut(s) 146, 162, 174, 650
Bsp143I GATC 6 cut(s) 268, 353, 407, 523, 598, 748
BspACI CCGC 1 cut(s) 812
BspANI GGCC 2 cut(s) 102, 619
BspCNI CTCAG 1 cut(s) 720
BspLI GGNNCC 3 cut(s) 80, 143, 153
BspPI GGATC 4 cut(s) 361, 402, 518, 593
BspT104I TTCGAA 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 78
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 103, 147
BssMI GATC 6 cut(s) 268, 353, 407, 523, 598, 748
BssNAI GTATAC 1 cut(s) 694
BssT1I CCWWGG 2 cut(s) 103, 147
Bst1107I GTATAC 1 cut(s) 694
Bst2UI CCWGG 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 693
BstBI TTCGAA 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 583
BstDEI CTNAG 3 cut(s) 272, 563, 728
BstF5I GGATG 1 cut(s) 762
BstKTI GATC 6 cut(s) 271, 356, 410, 526, 601, 751
BstMAI GTCTC 1 cut(s) 499
BstMBI GATC 6 cut(s) 268, 353, 407, 523, 598, 748
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 407
BstXI CCANNNNNNTGG 1 cut(s) 162
BstYI RGATCY 1 cut(s) 407
BstZ17I GTATAC 1 cut(s) 694
BsuI GTATCC 1 cut(s) 565
BsuRI GGCC 2 cut(s) 102, 619
BtsCI GGATG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 160, 372, 669
Cac8I GCNNGC 1 cut(s) 583
Cfr13I GGNCC 2 cut(s) 152, 809
Csp6I GTAC 2 cut(s) 570, 704
CviAII CATG 4 cut(s) 277, 311, 457, 752
CviQI GTAC 2 cut(s) 570, 704
DdeI CTNAG 3 cut(s) 272, 563, 728
DpnI GATC 6 cut(s) 270, 355, 409, 525, 600, 750
DpnII GATC 6 cut(s) 268, 353, 407, 523, 598, 748
EaeI YGGCCR 1 cut(s) 617
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 103, 147
Eco24I GRGCYC 3 cut(s) 146, 174, 650
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 2 cut(s) 152, 809
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 26, 693
EcoICRI GAGCTC 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 74
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 2 cut(s) 103, 147
EcoT38I GRGCYC 3 cut(s) 146, 174, 650
ErhI CCWWGG 2 cut(s) 103, 147
FaeI CATG 4 cut(s) 280, 314, 460, 755
FaqI GGGAC 1 cut(s) 165
FatI CATG 4 cut(s) 276, 310, 456, 751
FblI GTMKAC 1 cut(s) 693
FokI GGATG 1 cut(s) 769
FriOI GRGCYC 3 cut(s) 146, 174, 650
FspBI CTAG 2 cut(s) 243, 603
HaeIII GGCC 2 cut(s) 102, 619
Hin1II CATG 4 cut(s) 280, 314, 460, 755
HinfI GANTC 2 cut(s) 233, 331
HphI GGTGA 2 cut(s) 356, 455
Hpy166II GTNNAC 2 cut(s) 463, 694
Hpy188I TCNGA 3 cut(s) 89, 98, 415
Hpy188III TCNNGA 4 cut(s) 258, 521, 627, 655
Hpy8I GTNNAC 2 cut(s) 463, 694
HpyAV CCTTC 4 cut(s) 181, 300, 308, 668
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 5 cut(s) 195, 299, 454, 669, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 272, 563, 728
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 4 cut(s) 280, 314, 460, 755
Kzo9I GATC 6 cut(s) 268, 353, 407, 523, 598, 748
LmnI GCTCC 2 cut(s) 141, 687
LpnPI CCDG 6 cut(s) 61, 88, 148, 168, 336, 485
LweI GCATC 1 cut(s) 337
MaeI CTAG 2 cut(s) 243, 603
MaeII ACGT 1 cut(s) 9
MalI GATC 6 cut(s) 270, 355, 409, 525, 600, 750
MboI GATC 6 cut(s) 268, 353, 407, 523, 598, 748
MboII GAAGA 5 cut(s) 32, 59, 125, 191, 710
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 4 cut(s) 146, 162, 174, 650
MlsI TGGCCA 1 cut(s) 619
MluCI AATT 5 cut(s) 32, 206, 357, 449, 735
MluNI TGGCCA 1 cut(s) 619
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 9 cut(s) 92, 334, 421, 568, 571, 672, 694, 723, 789
Mox20I TGGCCA 1 cut(s) 619
MscI TGGCCA 1 cut(s) 619
MseI TTAA 5 cut(s) 6, 21, 426, 608, 783
Msp20I TGGCCA 1 cut(s) 619
MspA1I CMGCKG 1 cut(s) 727
MspR9I CCNGG 1 cut(s) 76
Mva1269I GAATGC 3 cut(s) 197, 244, 766
MvaI CCWGG 1 cut(s) 76
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 6 cut(s) 268, 353, 407, 523, 598, 748
NlaIII CATG 4 cut(s) 280, 314, 460, 755
NlaIV GGNNCC 3 cut(s) 80, 143, 153
NspV TTCGAA 1 cut(s) 236
PctI GAATGC 3 cut(s) 197, 244, 766
PfeI GAWTC 2 cut(s) 233, 331
PflMI CCANNNNNTGG 1 cut(s) 109
PshBI ATTAAT 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 3 cut(s) 80, 143, 153
PspPI GGNCC 2 cut(s) 152, 809
PsuI RGATCY 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 727
RsaI GTAC 2 cut(s) 571, 705
RsaNI GTAC 2 cut(s) 570, 704
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 5 cut(s) 6, 21, 426, 608, 783
Sau3AI GATC 6 cut(s) 268, 353, 407, 523, 598, 748
Sau96I GGNCC 2 cut(s) 152, 809
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 4 cut(s) 146, 162, 174, 650
SetI ASST 8 cut(s) 12, 174, 284, 319, 440, 626, 705, 729
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 236
SinI GGWCC 2 cut(s) 152, 809
SmlI CTYRAG 2 cut(s) 173, 256
SmoI CTYRAG 2 cut(s) 173, 256
Sse9I AATT 5 cut(s) 32, 206, 357, 449, 735
SsiI CCGC 1 cut(s) 812
SspMI CTAG 2 cut(s) 243, 603
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 1 cut(s) 74
StyI CCWWGG 2 cut(s) 103, 147
TaiI ACGT 1 cut(s) 12
TaqI TCGA 3 cut(s) 236, 329, 747
TasI AATT 5 cut(s) 32, 206, 357, 449, 735
TfiI GAWTC 2 cut(s) 233, 331
Tru1I TTAA 5 cut(s) 6, 21, 426, 608, 783
Tru9I TTAA 5 cut(s) 6, 21, 426, 608, 783
TscAI CASTG 3 cut(s) 167, 379, 676
TspDTI ATGAA 4 cut(s) 320, 327, 412, 773
TspRI CASTG 3 cut(s) 167, 379, 676
Van91I CCANNNNNTGG 1 cut(s) 109
VpaK11BI GGWCC 2 cut(s) 152, 809
VspI ATTAAT 1 cut(s) 426
XapI RAATTY 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 2 cut(s) 243, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.