Rw3G015340

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001343
Physical Location & Seq
Reverse (-)
15784283 .. 15785796
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015340.1

Sequence Viewer

Length: 825 bp
ATGCTATATCTATTATTCATTGCAGACGTAGTGATGGTTAAATCAAAGAAAGTCCGTCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACCCCATCAGACCCGTCATGGAAGAAATAGGGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAGAGCACTGGGGACAGCTCAAGGAAGAATGGAAGGAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTGGATTCGAATGCTCGAAAAGCAATCTCAAGAGTAGATGATCTTAAAATGAGCTATAAGCCTTCAAATGCAACCATGAAGCATGAAGGTTATGCTATTTCGATTCAATGGGAGGTTGATGCTGGATCAATTCAAATAAATGGCACTGATTACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCCTTAGTGTTACAAAGGAGGCACGCTTGGGAGTGATTGATCCAAGTTTAATAAAATGGCCAAGCTCACGATTTTACAGATACATGGGCTCACTCACGACCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTATACGAAGAGGTACATTCTGTTTCAAAAGAACAGCTGGGGCTAATAGAACAAGTCGATCATGGGATGAATGCAAGACCATTACAGCCTCTTAACAATCGAGGTATCACGCTCTTTGGTCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

274

Amino Acids

31.36

Weight (kDa)

6.8

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 52 - 269 8e-37 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 699
AciI CCGC 1 cut(s) 818
AclWI GGATC 4 cut(s) 367, 408, 524, 599
AcoI YGGCCR 1 cut(s) 623
AcuI CTGAAG 2 cut(s) 44, 699
AfaI GTAC 1 cut(s) 711
AgsI TTSAA 4 cut(s) 300, 341, 368, 723
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 4 cut(s) 178, 288, 630, 733
AluI AGCT 4 cut(s) 178, 288, 630, 733
Alw21I GWGCWC 1 cut(s) 168
AlwI GGATC 4 cut(s) 367, 408, 524, 599
AoxI GGCC 1 cut(s) 623
AseI ATTAAT 1 cut(s) 432
AspS9I GGNCC 2 cut(s) 158, 815
AsuHPI GGTGA 1 cut(s) 461
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 2 cut(s) 158, 815
BalI TGGCCA 1 cut(s) 625
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 2 cut(s) 152, 656
BbsI GAAGAC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 3 cut(s) 28, 110, 200
BciT130I CCWGG 1 cut(s) 94
BciVI GTATCC 1 cut(s) 571
BfmI CTRYAG 1 cut(s) 495
BfuI GTATCC 1 cut(s) 571
Bme1390I CCNGG 1 cut(s) 94
Bme18I GGWCC 2 cut(s) 158, 815
BmgT120I GGNCC 2 cut(s) 158, 815
BmiI GGNNCC 3 cut(s) 98, 149, 159
BmrFI CCNGG 1 cut(s) 94
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 1 cut(s) 343
BmuI ACTGGG 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 50
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 2 cut(s) 164, 247
BsaJI CCNNGG 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bse1I ACTGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 768
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 1 cut(s) 173
BseYI CCCAGC 1 cut(s) 733
BshFI GGCC 1 cut(s) 625
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 168
BslFI GGGAC 2 cut(s) 171, 186
BsmFI GGGAC 2 cut(s) 171, 186
BsmI GAATGC 4 cut(s) 203, 250, 503, 772
BsnI GGCC 1 cut(s) 625
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 3 cut(s) 152, 168, 656
Bsp143I GATC 6 cut(s) 274, 359, 413, 529, 604, 754
BspACI CCGC 1 cut(s) 818
BspANI GGCC 1 cut(s) 625
BspLI GGNNCC 3 cut(s) 98, 149, 159
BspPI GGATC 4 cut(s) 367, 408, 524, 599
BspT104I TTCGAA 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 6 cut(s) 274, 359, 413, 529, 604, 754
BssNAI GTATAC 1 cut(s) 700
BssT1I CCWWGG 1 cut(s) 153
Bst1107I GTATAC 1 cut(s) 700
Bst2UI CCWGG 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 699
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 1 cut(s) 569
BstF5I GGATG 1 cut(s) 768
BstKTI GATC 6 cut(s) 277, 362, 416, 532, 607, 757
BstMBI GATC 6 cut(s) 274, 359, 413, 529, 604, 754
BstMWI GCNNNNNNNGC 2 cut(s) 254, 500
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 92
BstSFI CTRYAG 1 cut(s) 495
BstV2I GAAGAC 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 413
BstXI CCANNNNNNTGG 1 cut(s) 168
BstYI RGATCY 1 cut(s) 413
BstZ17I GTATAC 1 cut(s) 700
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 1 cut(s) 625
BtsCI GGATG 1 cut(s) 768
BtsIMutI CAGTG 3 cut(s) 166, 378, 675
Cac8I GCNNGC 1 cut(s) 589
Cfr13I GGNCC 2 cut(s) 158, 815
Csp6I GTAC 1 cut(s) 710
CviAII CATG 6 cut(s) 115, 310, 317, 463, 649, 758
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 1 cut(s) 569
DpnI GATC 6 cut(s) 276, 361, 415, 531, 606, 756
DpnII GATC 6 cut(s) 274, 359, 413, 529, 604, 754
EaeI YGGCCR 1 cut(s) 623
Eam1104I CTCTTC 1 cut(s) 699
EarI CTCTTC 1 cut(s) 699
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 2 cut(s) 152, 656
Eco47I GGWCC 2 cut(s) 158, 815
Eco57I CTGAAG 2 cut(s) 44, 699
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 2 cut(s) 152, 656
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 6 cut(s) 118, 313, 320, 466, 652, 761
FalI AAGNNNNNCTT 2 cut(s) 42, 74
FaqI GGGAC 2 cut(s) 171, 186
FatI CATG 6 cut(s) 114, 309, 316, 462, 648, 757
FblI GTMKAC 1 cut(s) 699
FokI GGATG 1 cut(s) 775
FriOI GRGCYC 2 cut(s) 152, 656
GsaI CCCAGC 1 cut(s) 737
HaeIII GGCC 1 cut(s) 625
Hin1II CATG 6 cut(s) 118, 313, 320, 466, 652, 761
HinfI GANTC 2 cut(s) 239, 337
HphI GGTGA 1 cut(s) 461
Hpy166II GTNNAC 2 cut(s) 469, 700
Hpy188I TCNGA 2 cut(s) 107, 421
Hpy188III TCNNGA 4 cut(s) 264, 527, 633, 661
Hpy8I GTNNAC 2 cut(s) 469, 700
HpyAV CCTTC 4 cut(s) 187, 306, 314, 674
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 6 cut(s) 23, 201, 305, 460, 675, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 500
HpyF3I CTNAG 1 cut(s) 569
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 6 cut(s) 118, 313, 320, 466, 652, 761
Kzo9I GATC 6 cut(s) 274, 359, 413, 529, 604, 754
LmnI GCTCC 2 cut(s) 147, 693
LpnPI CCDG 8 cut(s) 79, 106, 154, 174, 342, 491, 551, 719
LweI GCATC 1 cut(s) 343
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 574
MalI GATC 6 cut(s) 276, 361, 415, 531, 606, 756
MboI GATC 6 cut(s) 274, 359, 413, 529, 604, 754
MboII GAAGA 5 cut(s) 50, 77, 131, 197, 716
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 3 cut(s) 152, 168, 656
MlsI TGGCCA 1 cut(s) 625
MluCI AATT 3 cut(s) 212, 363, 455
MluNI TGGCCA 1 cut(s) 625
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 8 cut(s) 340, 427, 574, 577, 678, 700, 791, 795
Mox20I TGGCCA 1 cut(s) 625
MscI TGGCCA 1 cut(s) 625
MseI TTAA 5 cut(s) 39, 279, 432, 614, 789
Msp20I TGGCCA 1 cut(s) 625
MspA1I CMGCKG 1 cut(s) 733
MspR9I CCNGG 1 cut(s) 94
Mva1269I GAATGC 4 cut(s) 203, 250, 503, 772
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 254, 500
NdeII GATC 6 cut(s) 274, 359, 413, 529, 604, 754
NlaIII CATG 6 cut(s) 118, 313, 320, 466, 652, 761
NlaIV GGNNCC 3 cut(s) 98, 149, 159
NspV TTCGAA 1 cut(s) 242
PctI GAATGC 4 cut(s) 203, 250, 503, 772
PfeI GAWTC 2 cut(s) 239, 337
PshBI ATTAAT 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 3 cut(s) 98, 149, 159
PspPI GGNCC 2 cut(s) 158, 815
PsuI RGATCY 1 cut(s) 413
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
SaqAI TTAA 5 cut(s) 39, 279, 432, 614, 789
Sau3AI GATC 6 cut(s) 274, 359, 413, 529, 604, 754
Sau96I GGNCC 2 cut(s) 158, 815
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 3 cut(s) 152, 168, 656
SetI ASST 9 cut(s) 30, 180, 290, 325, 351, 632, 711, 735, 802
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 2 cut(s) 158, 815
SmlI CTYRAG 2 cut(s) 179, 262
SmoI CTYRAG 2 cut(s) 179, 262
Sse9I AATT 3 cut(s) 212, 363, 455
SsiI CCGC 1 cut(s) 818
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 153
TaiI ACGT 1 cut(s) 30
TaqI TCGA 5 cut(s) 242, 250, 335, 753, 796
TasI AATT 3 cut(s) 212, 363, 455
TfiI GAWTC 2 cut(s) 239, 337
Tru1I TTAA 5 cut(s) 39, 279, 432, 614, 789
Tru9I TTAA 5 cut(s) 39, 279, 432, 614, 789
TscAI CASTG 3 cut(s) 173, 385, 682
TspDTI ATGAA 5 cut(s) 7, 326, 333, 418, 779
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 3 cut(s) 173, 385, 682
VpaK11BI GGWCC 2 cut(s) 158, 815
VspI ATTAAT 1 cut(s) 432
XmiI GTMKAC 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.