Rroxscaffold_6G00410460

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001343
Physical Location & Seq
Forward (+)
32891394 .. 32894518
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410460.1

Sequence Viewer

Length: 960 bp
ATGCTATTTAAGACCAGTCCCCCATTGGAGGAACTCGACCTTGGTGAGAAGCAAAACACTCACGACGTAGTGATGGTTAAATCAAAGAAAGTCCATCTTCAGTTGTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACCCCATCAAACCAGTCAGAGGCGAAGGAGTGGAAGAAATAGAGTATGATTATAGGCAAGGGAGCCTTTTTGGTGCAGTGGAAGAAATAGAATATGATTATAGGCAAGGGAGCCTTTTTGGTGGAGTGGAAGTAATGGAGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAACGAAGAATTGGAGGAATGCAAAGATGGAAAATTACAATCGCCAATAAATTTGTTGGATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAAGCCTTCAAAAGCAACTATGAAGCATGAACACCATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGATTACTTGCTCAAAAGATGTCATTGGCACAGACCCTCTGAACATTTCATTAATGGCAGAAGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGAGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACCATGTATCCTCCCTAAGTGGTAGAAAGAAGGCACGCTTGGAAGTGATTAATCCAAGTTTAATAAAATGGCCAAGCTCACGATTTTATAGATATGTGGGCTCACTCACGACCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTACATTCTGTTTCAAAAGAACAGCTGGGGCTAATTGAACAAGTCGATCGTGGGATGAATGCAAGACCATTACAGCCCCTCAACAATCGAGACATCACGCTCTTAGGTCTGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

319

Amino Acids

36.47

Weight (kDa)

6.17

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 101 - 314 6.3e-33 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 135
AclWI GGATC 2 cut(s) 514, 671
AcoI YGGCCR 1 cut(s) 770
AcsI RAATTY 1 cut(s) 376
AcuI CTGAAG 2 cut(s) 83, 846
AfaI GTAC 1 cut(s) 846
AfiI CCNNNNNNNGG 2 cut(s) 28, 155
AgsI TTSAA 5 cut(s) 447, 488, 515, 858, 881
AjnI CCWGG 1 cut(s) 131
AjuI GAANNNNNNNTTGG 4 cut(s) 24, 56, 722, 754
AluBI AGCT 4 cut(s) 325, 435, 777, 868
AluI AGCT 4 cut(s) 325, 435, 777, 868
Alw21I GWGCWC 2 cut(s) 315, 327
Alw26I GTCTC 1 cut(s) 927
AlwI GGATC 2 cut(s) 514, 671
AoxI GGCC 1 cut(s) 770
ApoI RAATTY 1 cut(s) 376
AseI ATTAAT 2 cut(s) 579, 750
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 3 cut(s) 56, 509, 608
AsuII TTCGAA 1 cut(s) 389
AvaII GGWCC 1 cut(s) 305
BalI TGGCCA 1 cut(s) 772
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 3 cut(s) 299, 327, 803
Bbv12I GWGCWC 2 cut(s) 315, 327
BccI CCATC 4 cut(s) 67, 102, 149, 347
BciT130I CCWGG 1 cut(s) 133
BciVI GTATCC 1 cut(s) 718
BcoDI GTCTC 1 cut(s) 927
BfaI CTAG 1 cut(s) 396
BfmI CTRYAG 2 cut(s) 642, 953
BfuI GTATCC 1 cut(s) 718
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 5 cut(s) 137, 200, 248, 296, 306
BmrFI CCNGG 1 cut(s) 133
BmrI ACTGGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 490
BmuI ACTGGG 1 cut(s) 325
Bpu14I TTCGAA 1 cut(s) 389
BpuEI CTTGAG 1 cut(s) 394
BsaJI CCNNGG 2 cut(s) 40, 300
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 799, 829
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 155
Bse1I ACTGG 3 cut(s) 15, 149, 320
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 2 cut(s) 40, 300
BseGI GGATG 1 cut(s) 903
BseLI CCNNNNNNNGG 2 cut(s) 28, 155
BseNI ACTGG 3 cut(s) 15, 149, 320
BseYI CCCAGC 1 cut(s) 868
BsgI GTGCAG 1 cut(s) 231
Bsh1285I CGRYCG 1 cut(s) 892
BshFI GGCC 1 cut(s) 772
BshNI GGYRCC 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 892
BsiHKAI GWGCWC 2 cut(s) 315, 327
BslFI GGGAC 2 cut(s) 3, 318
BslI CCNNNNNNNGG 2 cut(s) 28, 155
BsmAI GTCTC 1 cut(s) 927
BsmFI GGGAC 2 cut(s) 3, 318
BsmI GAATGC 4 cut(s) 350, 397, 650, 907
BsnI GGCC 1 cut(s) 772
Bsp119I TTCGAA 1 cut(s) 389
Bsp1286I GDGCHC 4 cut(s) 299, 315, 327, 803
Bsp143I GATC 4 cut(s) 421, 506, 676, 889
BspANI GGCC 1 cut(s) 772
BspLI GGNNCC 5 cut(s) 137, 200, 248, 296, 306
BspMAI CTGCAG 1 cut(s) 957
BspPI GGATC 2 cut(s) 514, 671
BspT104I TTCGAA 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 135
BsrI ACTGG 3 cut(s) 15, 149, 320
BssECI CCNNGG 2 cut(s) 40, 300
BssMI GATC 4 cut(s) 421, 506, 676, 889
BssT1I CCWWGG 2 cut(s) 40, 300
Bst2UI CCWGG 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 389
BstC8I GCNNGC 1 cut(s) 736
BstDEI CTNAG 2 cut(s) 716, 946
BstF5I GGATG 1 cut(s) 903
BstKTI GATC 4 cut(s) 424, 509, 679, 892
BstMAI GTCTC 1 cut(s) 927
BstMBI GATC 4 cut(s) 421, 506, 676, 889
BstMCI CGRYCG 1 cut(s) 892
BstMWI GCNNNNNNNGC 2 cut(s) 401, 647
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 2 cut(s) 642, 953
BstXI CCANNNNNNTGG 1 cut(s) 315
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 1 cut(s) 772
BtsCI GGATG 1 cut(s) 903
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 4 cut(s) 219, 313, 525, 822
Cac8I GCNNGC 1 cut(s) 736
Cfr13I GGNCC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 845
CviAII CATG 5 cut(s) 430, 464, 473, 610, 704
CviQI GTAC 1 cut(s) 845
DdeI CTNAG 2 cut(s) 716, 946
DpnI GATC 4 cut(s) 423, 508, 678, 891
DpnII GATC 4 cut(s) 421, 506, 676, 889
EaeI YGGCCR 1 cut(s) 770
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 2 cut(s) 40, 300
Eco24I GRGCYC 3 cut(s) 299, 327, 803
Eco47I GGWCC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 2 cut(s) 83, 846
EcoICRI GAGCTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 131
EcoT14I CCWWGG 2 cut(s) 40, 300
EcoT38I GRGCYC 3 cut(s) 299, 327, 803
ErhI CCWWGG 2 cut(s) 40, 300
FaeI CATG 5 cut(s) 433, 467, 476, 613, 707
FalI AAGNNNNNCTT 8 cut(s) 81, 113, 186, 218, 234, 266, 722, 754
FaqI GGGAC 2 cut(s) 3, 318
FatI CATG 5 cut(s) 429, 463, 472, 609, 703
FokI GGATG 1 cut(s) 910
FriOI GRGCYC 3 cut(s) 299, 327, 803
FspBI CTAG 1 cut(s) 396
GsaI CCCAGC 1 cut(s) 872
HaeIII GGCC 1 cut(s) 772
Hin1II CATG 5 cut(s) 433, 467, 476, 613, 707
HinfI GANTC 2 cut(s) 386, 484
HphI GGTGA 3 cut(s) 56, 509, 608
Hpy166II GTNNAC 1 cut(s) 616
Hpy188I TCNGA 2 cut(s) 155, 568
Hpy188III TCNNGA 6 cut(s) 62, 411, 674, 780, 808, 932
Hpy8I GTNNAC 1 cut(s) 616
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 4 cut(s) 155, 453, 724, 821
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 6 cut(s) 212, 348, 607, 822, 905, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 647
HpyF3I CTNAG 2 cut(s) 716, 946
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 5 cut(s) 433, 467, 476, 613, 707
Kzo9I GATC 4 cut(s) 421, 506, 676, 889
LmnI GCTCC 4 cut(s) 198, 246, 294, 840
LweI GCATC 1 cut(s) 490
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 66
MalI GATC 4 cut(s) 423, 508, 678, 891
MboI GATC 4 cut(s) 421, 506, 676, 889
MboII GAAGA 5 cut(s) 89, 116, 182, 230, 344
MhlI GDGCHC 4 cut(s) 299, 315, 327, 803
MlsI TGGCCA 1 cut(s) 772
MluCI AATT 6 cut(s) 335, 359, 376, 510, 602, 876
MluNI TGGCCA 1 cut(s) 772
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 9 cut(s) 22, 149, 334, 487, 574, 659, 721, 825, 932
Mox20I TGGCCA 1 cut(s) 772
MscI TGGCCA 1 cut(s) 772
MseI TTAA 6 cut(s) 9, 78, 426, 579, 750, 761
Msp20I TGGCCA 1 cut(s) 772
MspA1I CMGCKG 1 cut(s) 868
MspR9I CCNGG 1 cut(s) 133
Mva1269I GAATGC 4 cut(s) 350, 397, 650, 907
MvaI CCWGG 1 cut(s) 133
MwoI GCNNNNNNNGC 2 cut(s) 401, 647
NdeII GATC 4 cut(s) 421, 506, 676, 889
NlaIII CATG 5 cut(s) 433, 467, 476, 613, 707
NlaIV GGNNCC 5 cut(s) 137, 200, 248, 296, 306
NspV TTCGAA 1 cut(s) 389
PctI GAATGC 4 cut(s) 350, 397, 650, 907
PfeI GAWTC 2 cut(s) 386, 484
Ple19I CGATCG 1 cut(s) 892
PshBI ATTAAT 2 cut(s) 579, 750
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 131
PspFI CCCAGC 1 cut(s) 868
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 5 cut(s) 137, 200, 248, 296, 306
PspPI GGNCC 1 cut(s) 305
PstI CTGCAG 1 cut(s) 957
PvuI CGATCG 1 cut(s) 892
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 1 cut(s) 846
RsaNI GTAC 1 cut(s) 845
SacI GAGCTC 1 cut(s) 327
SaqAI TTAA 6 cut(s) 9, 78, 426, 579, 750, 761
Sau3AI GATC 4 cut(s) 421, 506, 676, 889
Sau96I GGNCC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 4 cut(s) 299, 315, 327, 803
SetI ASST 7 cut(s) 42, 69, 327, 437, 779, 870, 952
SfaNI GCATC 1 cut(s) 490
SfcI CTRYAG 2 cut(s) 642, 953
SfuI TTCGAA 1 cut(s) 389
SinI GGWCC 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 409
SmoI CTYRAG 1 cut(s) 409
Sse9I AATT 6 cut(s) 335, 359, 376, 510, 602, 876
SspMI CTAG 1 cut(s) 396
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 2 cut(s) 40, 300
TaiI ACGT 1 cut(s) 69
TaqI TCGA 5 cut(s) 36, 389, 482, 888, 931
TasI AATT 6 cut(s) 335, 359, 376, 510, 602, 876
TfiI GAWTC 2 cut(s) 386, 484
Tru1I TTAA 6 cut(s) 9, 78, 426, 579, 750, 761
Tru9I TTAA 6 cut(s) 9, 78, 426, 579, 750, 761
TscAI CASTG 4 cut(s) 219, 320, 532, 829
TspDTI ATGAA 4 cut(s) 473, 480, 565, 914
TspRI CASTG 4 cut(s) 219, 320, 532, 829
VpaK11BI GGWCC 1 cut(s) 305
VspI ATTAAT 2 cut(s) 579, 750
XapI RAATTY 1 cut(s) 376
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XspI CTAG 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.