FvH4_6g44570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000643
Physical Location & Seq
Forward (+)
34325530 .. 34327353
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44570.t1

Sequence Viewer

Length: 1293 bp
ATGAGTGTTCTTGAATACCCAGATGCGATTAACGCGCCGGAGCTTCAGGTCTGGAACAACGCCGCCTTCGACGACAACGAAGGTTCTGGATTCTCCGCCGTCAAACCCTCCTGGTCTTTCGAGTCCGACTGCAGCAAGGAGAATCTGAGCCCGGTTCCGAAGACCCCGACTTTCGCGGCGTCTAATTCGGTGCCGTTCAAGCCGCTGAACGGTGCCTCGCGGCGGTTGTCGGTGGCGCCGAGGAAGATGGGGTTTGAGGTTGAAGACTTGGGGAAGATCGATTCGGAGATTGAGGAGATCGAGAAGGAGATAAGCCGATTGAATTCGAAGCTTGAGTTGCTGAAGCTCGAGAAGGCCCAGAAGGCGGCGGAGCGGCGCGGCAGAGTGGTGGCTGCGAAGTTCATGGAGCCGCCGGCGGCGAAGCAGAGTGTGAAGAATTCCGATGGGTTGAGAAAGATTGAGGAGTCTTTGAATTTGAGCGCCAGGCCGAAGGTGAATCGGAGAGGTATGAGTTTGGGGCCGTCGGAGATTATCGCCGGAGCAGCGTTCCGGCGGCCGGGTAAGCTTGAGATAACCCCTGGTCAGAAAATACAGGACCGCCGGAAGTCTTGCTTCTGGAAGCTTCAGGACATTGATGAACTGAGGGTTACTAAAGAGAGGGGGAAGAGCAGTAGTCTTAGCCCGAAATCGAGGAAAACTGCGTCGAAAACCGGGGTTTCGAAACAGGCTGCAACTACAATTGGGGGTTCTAAGAGGCCGGTGAAGATGGTAGACAAGGTTCTTGCTTCAATTGAGCCCAAGAAGCTTTTTAAAGAAGGGGAGAAGTCTGTGCCTAATAAGAAATCAGTCAAGGCTGGGAGGGTTGTGGCAAGCAGGTACAATCAGATTGGGAGCTCTTCAGTTGCGGCGGCAGATGGTAGGAAAAGGTCTTTGCCGGATGATGATAAGGAAGATGGGAACCGGTGTGAGAAGAAGCGCGTGTCATTGGTGGGGAAGCCGCGTGGCATTGGGAGGGAGGGATCTAGGAGCGTTGGAGCAGAGAGTAGGATGAAGAAGAGGTGGGAGATTCCTAGTGAGGTGGTGGTGTTCCAGGGTGTGGAGGAGGATGGTGTTGCTGCAATTGATGTTCTGCCGAAGATTAGGACTGTTAGATGTGTTAATGACACGCCAAGGGGGTCAGGGCCGGCGAAAAGGGCGGCTGAATTGGTTGGGATGAAGAAGTATTTTAGCAACAGTGATGAGTTCTGCCAGGAGTTGAGTTTTGCAGAAGAGGAGGATGGTGAGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

431

Amino Acids

47.19

Weight (kDa)

9.55

Isoelectric Point (pI)

57.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 864
AccB1I GGYRCC 3 cut(s) 190, 212, 235
AccB7I CCANNNNNTGG 1 cut(s) 1096
AccBSI CCGCTC 1 cut(s) 373
AccI GTMKAC 1 cut(s) 771
AccII CGCG 6 cut(s) 35, 176, 220, 378, 978, 1000
AclWI GGATC 1 cut(s) 1027
AcoI YGGCCR 1 cut(s) 554
AcsI RAATTY 3 cut(s) 322, 436, 472
AcuI CTGAAG 4 cut(s) 29, 362, 608, 882
AcyI GRCGYC 2 cut(s) 179, 236
AfaI GTAC 1 cut(s) 878
AfiI CCNNNNNNNGG 5 cut(s) 209, 222, 364, 556, 1096
AgeI ACCGGT 1 cut(s) 960
AgsI TTSAA 6 cut(s) 14, 199, 263, 322, 472, 789
AjnI CCWGG 5 cut(s) 110, 482, 577, 1089, 1248
AluBI AGCT 7 cut(s) 43, 331, 346, 565, 622, 805, 894
AluI AGCT 7 cut(s) 43, 331, 346, 565, 622, 805, 894
Alw21I GWGCWC 1 cut(s) 896
AlwI GGATC 1 cut(s) 1027
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 6 cut(s) 354, 485, 518, 554, 755, 1181
ApeKI GCWGC 5 cut(s) 132, 392, 542, 728, 1115
ApoI RAATTY 3 cut(s) 322, 436, 472
AsiGI ACCGGT 1 cut(s) 960
AspLEI GCGC 5 cut(s) 37, 238, 378, 482, 978
AspS9I GGNCC 4 cut(s) 355, 518, 595, 1181
AsuC2I CCSGG 3 cut(s) 152, 558, 712
AsuHPI GGTGA 3 cut(s) 505, 772, 1292
AsuII TTCGAA 2 cut(s) 326, 719
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 595
BanI GGYRCC 3 cut(s) 190, 212, 235
BanII GRGCYC 3 cut(s) 152, 798, 896
BbsI GAAGAC 2 cut(s) 167, 270
Bbv12I GWGCWC 1 cut(s) 896
BbvI GCAGC 5 cut(s) 144, 379, 554, 715, 1102
BccI CCATC 7 cut(s) 241, 437, 760, 908, 947, 1100, 1271
BceAI ACGGC 3 cut(s) 83, 178, 505
BciT130I CCWGG 5 cut(s) 112, 484, 579, 1091, 1250
BcnI CCSGG 3 cut(s) 152, 558, 712
BfaI CTAG 2 cut(s) 1023, 1071
BfmI CTRYAG 1 cut(s) 130
BfoI RGCGCY 2 cut(s) 239, 483
BfuAI ACCTGC 1 cut(s) 864
BglI GCCNNNNNGGC 1 cut(s) 362
Bme1390I CCNGG 8 cut(s) 112, 152, 484, 558, 579, 712, 1091, 1250
Bme18I GGWCC 1 cut(s) 595
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 4 cut(s) 355, 518, 595, 1181
BmiI GGNNCC 7 cut(s) 156, 192, 214, 237, 408, 519, 959
BmrFI CCNGG 8 cut(s) 112, 152, 484, 558, 579, 712, 1091, 1250
BmsI GCATC 1 cut(s) 13
BpiI GAAGAC 2 cut(s) 167, 270
Bpu14I TTCGAA 2 cut(s) 326, 719
BpuEI CTTGAG 2 cut(s) 353, 587
BpuMI CCSGG 3 cut(s) 152, 558, 712
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 2 cut(s) 179, 236
BsaJI CCNNGG 5 cut(s) 239, 577, 711, 1090, 1169
BsaWI WCCGGW 1 cut(s) 960
Bsc4I CCNNNNNNNGG 5 cut(s) 209, 222, 364, 556, 1096
Bse118I RCCGGY 4 cut(s) 412, 757, 960, 1183
BseBI CCWGG 5 cut(s) 112, 484, 579, 1091, 1250
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 5 cut(s) 239, 577, 711, 1090, 1169
BseGI GGATG 5 cut(s) 943, 1053, 1111, 1218, 1282
BseLI CCNNNNNNNGG 5 cut(s) 209, 222, 364, 556, 1096
BseMII CTCAG 2 cut(s) 137, 632
BseRI GAGGAG 4 cut(s) 308, 476, 1115, 1286
BseX3I CGGCCG 1 cut(s) 554
BseXI GCAGC 5 cut(s) 144, 379, 554, 715, 1102
BseYI CCCAGC 1 cut(s) 854
Bsh1236I CGCG 6 cut(s) 35, 176, 220, 378, 978, 1000
Bsh1285I CGRYCG 1 cut(s) 557
BshFI GGCC 6 cut(s) 356, 487, 520, 556, 757, 1183
BshNI GGYRCC 3 cut(s) 190, 212, 235
BshTI ACCGGT 1 cut(s) 960
BshVI ATCGAT 1 cut(s) 279
BsiEI CGRYCG 1 cut(s) 557
BsiHKAI GWGCWC 1 cut(s) 896
BsiHKCI CYCGRG 1 cut(s) 347
BslI CCNNNNNNNGG 5 cut(s) 209, 222, 364, 556, 1096
BsnI GGCC 6 cut(s) 356, 487, 520, 556, 757, 1183
BsoBI CYCGRG 1 cut(s) 347
Bsp119I TTCGAA 2 cut(s) 326, 719
Bsp1286I GDGCHC 3 cut(s) 152, 798, 896
Bsp143I GATC 3 cut(s) 276, 297, 1019
BspANI GGCC 6 cut(s) 356, 487, 520, 556, 757, 1183
BspCNI CTCAG 2 cut(s) 138, 633
BspDI ATCGAT 1 cut(s) 279
BspFNI CGCG 6 cut(s) 35, 176, 220, 378, 978, 1000
BspLI GGNNCC 7 cut(s) 156, 192, 214, 237, 408, 519, 959
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 1 cut(s) 864
BspPI GGATC 1 cut(s) 1027
BspQI GCTCTTC 2 cut(s) 659, 901
BspT104I TTCGAA 2 cut(s) 326, 719
BspT107I GGYRCC 3 cut(s) 190, 212, 235
BsrBI CCGCTC 1 cut(s) 373
BsrFI RCCGGY 4 cut(s) 412, 757, 960, 1183
BssAI RCCGGY 4 cut(s) 412, 757, 960, 1183
BssECI CCNNGG 5 cut(s) 239, 577, 711, 1090, 1169
BssMI GATC 3 cut(s) 276, 297, 1019
BssNI GRCGYC 2 cut(s) 179, 236
BssT1I CCWWGG 1 cut(s) 1169
Bst2UI CCWGG 5 cut(s) 112, 484, 579, 1091, 1250
Bst4CI ACNGT 3 cut(s) 212, 1147, 1235
Bst6I CTCTTC 4 cut(s) 659, 901, 1049, 1263
BstACI GRCGYC 2 cut(s) 179, 236
BstBI TTCGAA 2 cut(s) 326, 719
BstC8I GCNNGC 3 cut(s) 414, 871, 1185
BstDEI CTNAG 4 cut(s) 146, 641, 677, 750
BstF5I GGATG 5 cut(s) 943, 1053, 1111, 1218, 1282
BstFNI CGCG 6 cut(s) 35, 176, 220, 378, 978, 1000
BstH2I RGCGCY 2 cut(s) 239, 483
BstHHI GCGC 5 cut(s) 37, 238, 378, 482, 978
BstKTI GATC 3 cut(s) 279, 300, 1022
BstMBI GATC 3 cut(s) 276, 297, 1019
BstMCI CGRYCG 1 cut(s) 557
BstMWI GCNNNNNNNGC 8 cut(s) 32, 199, 337, 362, 542, 562, 802, 1193
BstNI CCWGG 5 cut(s) 112, 484, 579, 1091, 1250
BstSCI CCNGG 8 cut(s) 110, 150, 482, 556, 577, 710, 1089, 1248
BstSFI CTRYAG 1 cut(s) 130
BstUI CGCG 6 cut(s) 35, 176, 220, 378, 978, 1000
BstV1I GCAGC 5 cut(s) 144, 379, 554, 715, 1102
BstV2I GAAGAC 2 cut(s) 167, 270
BstX2I RGATCY 1 cut(s) 1019
BstYI RGATCY 1 cut(s) 1019
BstZI CGGCCG 1 cut(s) 554
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 6 cut(s) 356, 487, 520, 556, 757, 1183
BsuTUI ATCGAT 1 cut(s) 279
BtsCI GGATG 5 cut(s) 943, 1053, 1111, 1218, 1282
BtsIMutI CAGTG 1 cut(s) 1240
BveI ACCTGC 1 cut(s) 864
Cac8I GCNNGC 3 cut(s) 414, 871, 1185
CfoI GCGC 5 cut(s) 37, 238, 378, 482, 978
Cfr10I RCCGGY 4 cut(s) 412, 757, 960, 1183
Cfr13I GGNCC 4 cut(s) 355, 518, 595, 1181
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 2 cut(s) 168, 690
Csp6I GTAC 1 cut(s) 877
CspAI ACCGGT 1 cut(s) 960
CviAII CATG 1 cut(s) 403
CviQI GTAC 1 cut(s) 877
DdeI CTNAG 4 cut(s) 146, 641, 677, 750
DinI GGCGCC 1 cut(s) 237
DpnI GATC 3 cut(s) 278, 299, 1021
DpnII GATC 3 cut(s) 276, 297, 1019
DraI TTTAAA 1 cut(s) 811
EaeI YGGCCR 1 cut(s) 554
EagI CGGCCG 1 cut(s) 554
Eam1104I CTCTTC 4 cut(s) 659, 901, 1049, 1263
EarI CTCTTC 4 cut(s) 659, 901, 1049, 1263
EciI GGCGGA 2 cut(s) 85, 383
Ecl136II GAGCTC 1 cut(s) 894
EclXI CGGCCG 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 1169
Eco24I GRGCYC 3 cut(s) 152, 798, 896
Eco47I GGWCC 1 cut(s) 595
Eco52I CGGCCG 1 cut(s) 554
Eco53kI GAGCTC 1 cut(s) 894
Eco57I CTGAAG 4 cut(s) 29, 362, 608, 882
Eco88I CYCGRG 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 894
EcoRI GAATTC 2 cut(s) 322, 436
EcoRII CCWGG 5 cut(s) 110, 482, 577, 1089, 1248
EcoT14I CCWWGG 1 cut(s) 1169
EcoT38I GRGCYC 3 cut(s) 152, 798, 896
EgeI GGCGCC 1 cut(s) 237
EheI GGCGCC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 1169
FaeI CATG 1 cut(s) 406
FaiI YATR 2 cut(s) 404, 509
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FatI CATG 1 cut(s) 402
FblI GTMKAC 1 cut(s) 771
FokI GGATG 5 cut(s) 950, 1060, 1118, 1225, 1289
FriOI GRGCYC 3 cut(s) 152, 798, 896
FspBI CTAG 2 cut(s) 1023, 1071
GlaI GCGC 5 cut(s) 36, 237, 377, 481, 977
GsaI CCCAGC 1 cut(s) 858
HaeII RGCGCY 2 cut(s) 239, 483
HaeIII GGCC 6 cut(s) 356, 487, 520, 556, 757, 1183
HgaI GACGC 2 cut(s) 168, 690
HhaI GCGC 5 cut(s) 37, 238, 378, 482, 978
Hin1I GRCGYC 2 cut(s) 179, 236
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 5 cut(s) 35, 236, 376, 480, 976
HinP1I GCGC 5 cut(s) 35, 236, 376, 480, 976
HindIII AAGCTT 4 cut(s) 329, 563, 620, 803
HinfI GANTC 7 cut(s) 90, 122, 142, 281, 464, 496, 1066
HphI GGTGA 3 cut(s) 505, 772, 1292
Hpy166II GTNNAC 1 cut(s) 772
Hpy188I TCNGA 9 cut(s) 127, 147, 159, 286, 442, 501, 526, 585, 885
Hpy188III TCNNGA 7 cut(s) 11, 52, 87, 301, 349, 616, 626
Hpy8I GTNNAC 1 cut(s) 772
Hpy99I CGWCG 3 cut(s) 74, 526, 706
HpyAV CCTTC 7 cut(s) 74, 76, 298, 346, 355, 484, 809
HpyCH4III ACNGT 3 cut(s) 212, 1147, 1235
HpyCH4V TGCA 4 cut(s) 132, 731, 1118, 1265
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 199, 337, 362, 542, 562, 802, 1193
HpyF3I CTNAG 4 cut(s) 146, 641, 677, 750
Hsp92I GRCGYC 2 cut(s) 179, 236
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 5 cut(s) 35, 236, 376, 480, 976
KasI GGCGCC 1 cut(s) 235
KroI GCCGGC 2 cut(s) 412, 1183
KroNI GCCGGC 2 cut(s) 414, 1185
Kzo9I GATC 3 cut(s) 276, 297, 1019
LguI GCTCTTC 2 cut(s) 659, 901
LmnI GCTCC 7 cut(s) 40, 370, 406, 539, 891, 1026, 1034
Lsp1109I GCAGC 5 cut(s) 144, 379, 554, 715, 1102
LweI GCATC 1 cut(s) 13
MaeI CTAG 2 cut(s) 1023, 1071
MaeIII GTNAC 1 cut(s) 646
MalI GATC 3 cut(s) 278, 299, 1021
MbiI CCGCTC 1 cut(s) 373
MboI GATC 3 cut(s) 276, 297, 1019
MfeI CAATTG 3 cut(s) 738, 789, 1119
MflI RGATCY 1 cut(s) 1019
MhlI GDGCHC 3 cut(s) 152, 798, 896
MluCI AATT 8 cut(s) 184, 322, 436, 472, 738, 789, 1119, 1202
Mly113I GGCGCC 1 cut(s) 236
MlyI GAGTC 2 cut(s) 131, 473
MmeI TCCRAC 3 cut(s) 150, 504, 1012
MreI CGCCGGCG 1 cut(s) 412
MroNI GCCGGC 2 cut(s) 412, 1183
MseI TTAA 3 cut(s) 30, 810, 1158
MspA1I CMGCKG 1 cut(s) 205
MspR9I CCNGG 8 cut(s) 112, 152, 484, 558, 579, 712, 1091, 1250
MunI CAATTG 3 cut(s) 738, 789, 1119
MvaI CCWGG 5 cut(s) 112, 484, 579, 1091, 1250
MvnI CGCG 6 cut(s) 35, 176, 220, 378, 978, 1000
MwoI GCNNNNNNNGC 8 cut(s) 32, 199, 337, 362, 542, 562, 802, 1193
NaeI GCCGGC 2 cut(s) 414, 1185
NarI GGCGCC 1 cut(s) 236
NciI CCSGG 3 cut(s) 152, 558, 712
NdeII GATC 3 cut(s) 276, 297, 1019
NgoMIV GCCGGC 2 cut(s) 412, 1183
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 7 cut(s) 156, 192, 214, 237, 408, 519, 959
NmeAIII GCCGAG 1 cut(s) 264
NspV TTCGAA 2 cut(s) 326, 719
PaeR7I CTCGAG 1 cut(s) 347
PciSI GCTCTTC 2 cut(s) 659, 901
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 75, 236
PdiI GCCGGC 2 cut(s) 414, 1185
PfeI GAWTC 5 cut(s) 90, 142, 281, 496, 1066
PflMI CCANNNNNTGG 1 cut(s) 1096
PinAI ACCGGT 1 cut(s) 960
PleI GAGTC 2 cut(s) 130, 472
PluTI GGCGCC 1 cut(s) 239
PpsI GAGTC 2 cut(s) 130, 472
Psp124BI GAGCTC 1 cut(s) 896
Psp6I CCWGG 5 cut(s) 110, 482, 577, 1089, 1248
PspFI CCCAGC 1 cut(s) 854
PspGI CCWGG 5 cut(s) 110, 482, 577, 1089, 1248
PspN4I GGNNCC 7 cut(s) 156, 192, 214, 237, 408, 519, 959
PspPI GGNCC 4 cut(s) 355, 518, 595, 1181
PsrI GAACNNNNNNTAC 2 cut(s) 762, 794
PstI CTGCAG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 1019
RsaI GTAC 1 cut(s) 878
RsaNI GTAC 1 cut(s) 877
SacI GAGCTC 1 cut(s) 896
SapI GCTCTTC 2 cut(s) 659, 901
SaqAI TTAA 3 cut(s) 30, 810, 1158
Sau3AI GATC 3 cut(s) 276, 297, 1019
Sau96I GGNCC 4 cut(s) 355, 518, 595, 1181
SchI GAGTC 2 cut(s) 131, 473
ScrFI CCNGG 8 cut(s) 112, 152, 484, 558, 579, 712, 1091, 1250
SduI GDGCHC 3 cut(s) 152, 798, 896
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 130
SfoI GGCGCC 1 cut(s) 237
Sfr274I CTCGAG 1 cut(s) 347
SfuI TTCGAA 2 cut(s) 326, 719
SgrAI CRCCGGYG 1 cut(s) 412
SinI GGWCC 1 cut(s) 595
SlaI CTCGAG 1 cut(s) 347
SmlI CTYRAG 3 cut(s) 332, 347, 566
SmoI CTYRAG 3 cut(s) 332, 347, 566
Sse9I AATT 8 cut(s) 184, 322, 436, 472, 738, 789, 1119, 1202
SspDI GGCGCC 1 cut(s) 235
SspMI CTAG 2 cut(s) 1023, 1071
SstI GAGCTC 1 cut(s) 896
StyD4I CCNGG 8 cut(s) 110, 150, 482, 556, 577, 710, 1089, 1248
StyI CCWWGG 1 cut(s) 1169
TaaI ACNGT 3 cut(s) 212, 1147, 1235
TaqI TCGA 9 cut(s) 69, 120, 279, 300, 326, 348, 689, 704, 719
TasI AATT 8 cut(s) 184, 322, 436, 472, 738, 789, 1119, 1202
TfiI GAWTC 5 cut(s) 90, 142, 281, 496, 1066
Tru1I TTAA 3 cut(s) 30, 810, 1158
Tru9I TTAA 3 cut(s) 30, 810, 1158
TscAI CASTG 1 cut(s) 1240
TseI GCWGC 5 cut(s) 132, 392, 542, 728, 1115
TspDTI ATGAA 4 cut(s) 391, 651, 1064, 1229
TspRI CASTG 1 cut(s) 1240
Van91I CCANNNNNTGG 1 cut(s) 1096
VpaK11BI GGWCC 1 cut(s) 595
XapI RAATTY 3 cut(s) 322, 436, 472
XhoI CTCGAG 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 2 cut(s) 1023, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.