Rroxscaffold_4G00288710

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000643
Physical Location & Seq
Reverse (-)
9394338 .. 9399015
4678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288710.1

Sequence Viewer

Length: 621 bp
ATGTCGTCCCCGCACGTCTTCCTCCGGGAACCGCCGGAGGCAGCCATGGTTCTCACAACTAAGCTTTGCCGCTTTAGTGGTGCCAAGATCTACCCCCGGATTTCCCTGTTCACAAATTCCAAGTGCAAGAGGTACTTTAACAACCGCCTCAAGCCATCCAAGATCACATGGACTGCCATGTTCGAAAGCGACAAGAAGGCAATTGGTTCTCTTCACTGTGCTTTGACTAATACATGCATCCCTATGTCTCACACTTGCTCCCTTACGCAGGGTGTTCATGATTTTGTTTTTGCATTACGAGACAAGTCTTGCAGTAGAATCTCAGCCAAAACAGTAAGGACCTTGAACTCGCATGCTTACAACCCAAATGAGGAGTATGCAGATGTGGAATCCACATTGCGTTCACATTTGGAATCTTTTCTAGAAACTGCAAGATCATTCAATATGATTTACACCAAGTTGTGTAAAAGAGACATACTCTTATCTACTACCTTGAAATGTCGTGCTTTTGCAGGAAATACGTCCCTGGACACACATGATGGAGGTACCCCACCCCAGCTCCATGGGTTCGGGCCGGGCCTGCAGCCAATCGCTTATTGGAGGCATATAAGATGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L24e

Protein Analysis

206

Amino Acids

23.29

Weight (kDa)

9.46

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L24e PF01246 20 - 66 1e-06 Ribosomal protein L24e
Plant_NMP1 PF06694 111 - 154 2.9e-13 Plant nuclear matrix protein 1 (NMP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 545
AccB1I GGYRCC 2 cut(s) 80, 545
AciI CCGC 4 cut(s) 11, 32, 70, 145
AcsI RAATTY 1 cut(s) 115
AfaI GTAC 2 cut(s) 134, 547
AfiI CCNNNNNNNGG 2 cut(s) 268, 370
AgsI TTSAA 3 cut(s) 346, 442, 496
AjiI CACGTC 1 cut(s) 16
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 2 cut(s) 64, 559
AluI AGCT 2 cut(s) 64, 559
Alw26I GTCTC 3 cut(s) 252, 294, 465
AoxI GGCC 2 cut(s) 572, 577
ApeKI GCWGC 2 cut(s) 41, 583
ApoI RAATTY 1 cut(s) 115
Asp700I GAANNNNTTC 1 cut(s) 417
Asp718I GGTACC 1 cut(s) 545
AspS9I GGNCC 3 cut(s) 339, 572, 577
AsuC2I CCSGG 3 cut(s) 26, 97, 576
AsuII TTCGAA 1 cut(s) 183
AvaII GGWCC 1 cut(s) 339
BanI GGYRCC 2 cut(s) 80, 545
BbsI GAAGAC 1 cut(s) 10
BbvI GCAGC 2 cut(s) 53, 595
BccI CCATC 2 cut(s) 163, 533
BciT130I CCWGG 1 cut(s) 527
BcnI CCSGG 3 cut(s) 26, 97, 576
BcoDI GTCTC 3 cut(s) 252, 294, 465
BfaI CTAG 1 cut(s) 422
BfmI CTRYAG 1 cut(s) 581
BglII AGATCT 1 cut(s) 87
BisI GCNGC 3 cut(s) 42, 70, 584
BlsI GCNGC 3 cut(s) 43, 71, 585
Bme1390I CCNGG 4 cut(s) 26, 97, 527, 576
Bme18I GGWCC 1 cut(s) 339
BmgBI CACGTC 1 cut(s) 16
BmgT120I GGNCC 3 cut(s) 339, 572, 577
BmiI GGNNCC 3 cut(s) 30, 82, 547
BmrFI CCNGG 4 cut(s) 26, 97, 527, 576
BmsI GCATC 2 cut(s) 246, 602
BpiI GAAGAC 1 cut(s) 10
BplI GAGNNNNNCTC 2 cut(s) 462, 494
Bpu14I TTCGAA 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 3 cut(s) 26, 97, 576
BsaJI CCNNGG 4 cut(s) 45, 95, 525, 562
BsaXI ACNNNNNCTCC 4 cut(s) 242, 272, 543, 573
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 370
Bse3DI GCAATG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 4 cut(s) 45, 95, 525, 562
BseGI GGATG 2 cut(s) 155, 237
BseLI CCNNNNNNNGG 2 cut(s) 268, 370
BseMI GCAATG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 386
BseXI GCAGC 2 cut(s) 53, 595
BseYI CCCAGC 1 cut(s) 555
BshFI GGCC 2 cut(s) 574, 579
BshNI GGYRCC 2 cut(s) 80, 545
BsiSI CCGG 4 cut(s) 25, 35, 97, 575
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 2 cut(s) 268, 370
BsmAI GTCTC 3 cut(s) 252, 294, 465
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 2 cut(s) 574, 579
Bsp119I TTCGAA 1 cut(s) 183
Bsp143I GATC 3 cut(s) 87, 162, 434
Bsp19I CCATGG 2 cut(s) 45, 562
BspACI CCGC 4 cut(s) 11, 32, 70, 145
BspANI GGCC 2 cut(s) 574, 579
BspCNI CTCAG 1 cut(s) 335
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 30, 82, 547
BspMAI CTGCAG 1 cut(s) 585
BspT104I TTCGAA 1 cut(s) 183
BspT107I GGYRCC 2 cut(s) 80, 545
BsrDI GCAATG 1 cut(s) 395
BssECI CCNNGG 4 cut(s) 45, 95, 525, 562
BssMI GATC 3 cut(s) 87, 162, 434
BssT1I CCWWGG 2 cut(s) 45, 562
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 2 cut(s) 218, 334
Bst6I CTCTTC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 354, 581
BstDEI CTNAG 2 cut(s) 60, 322
BstDSI CCRYGG 2 cut(s) 45, 562
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 2 cut(s) 155, 237
BstKTI GATC 3 cut(s) 90, 165, 437
BstMAI GTCTC 3 cut(s) 252, 294, 465
BstMBI GATC 3 cut(s) 87, 162, 434
BstMWI GCNNNNNNNGC 1 cut(s) 580
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 2 cut(s) 237, 356
BstSCI CCNGG 4 cut(s) 24, 95, 525, 574
BstSFI CTRYAG 1 cut(s) 581
BstV1I GCAGC 2 cut(s) 53, 595
BstV2I GAAGAC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 87
BstXI CCANNNNNNTGG 1 cut(s) 563
BstYI RGATCY 1 cut(s) 87
BsuRI GGCC 2 cut(s) 574, 579
BtgI CCRYGG 2 cut(s) 45, 562
BtrI CACGTC 1 cut(s) 16
BtsCI GGATG 2 cut(s) 155, 237
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 354, 581
CciI TCATGA 1 cut(s) 277
Cfr13I GGNCC 3 cut(s) 339, 572, 577
Csp6I GTAC 2 cut(s) 133, 546
CviAII CATG 8 cut(s) 46, 168, 178, 234, 278, 353, 536, 563
CviJI RGCY 8 cut(s) 44, 64, 154, 326, 559, 574, 579, 586
CviKI_1 RGCY 8 cut(s) 44, 64, 154, 326, 559, 574, 579, 586
CviQI GTAC 2 cut(s) 133, 546
DdeI CTNAG 2 cut(s) 60, 322
DpnI GATC 3 cut(s) 89, 164, 436
DpnII GATC 3 cut(s) 87, 162, 434
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 45, 562
Eco47I GGWCC 1 cut(s) 339
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 2 cut(s) 45, 562
EcoT22I ATGCAT 1 cut(s) 239
ErhI CCWWGG 2 cut(s) 45, 562
FaeI CATG 8 cut(s) 49, 171, 181, 237, 281, 356, 539, 566
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FaqI GGGAC 1 cut(s) 508
FatI CATG 8 cut(s) 45, 167, 177, 233, 277, 352, 535, 562
FauI CCCGC 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 42, 70, 584
FokI GGATG 2 cut(s) 142, 224
Fsp4HI GCNGC 3 cut(s) 42, 70, 584
FspBI CTAG 1 cut(s) 422
GluI GCNGC 3 cut(s) 42, 70, 584
GsaI CCCAGC 1 cut(s) 559
HaeIII GGCC 2 cut(s) 574, 579
HapII CCGG 4 cut(s) 25, 35, 97, 575
Hin1II CATG 8 cut(s) 49, 171, 181, 237, 281, 356, 539, 566
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 3 cut(s) 318, 389, 413
HpaII CCGG 4 cut(s) 25, 35, 97, 575
Hpy166II GTNNAC 2 cut(s) 111, 404
Hpy188III TCNNGA 2 cut(s) 278, 422
Hpy8I GTNNAC 2 cut(s) 111, 404
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 2 cut(s) 218, 334
HpyCH4IV ACGT 2 cut(s) 15, 521
HpyCH4V TGCA 8 cut(s) 126, 237, 293, 312, 380, 431, 512, 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 580
HpyF3I CTNAG 2 cut(s) 60, 322
HpySE526I ACGT 2 cut(s) 15, 521
Hsp92II CATG 8 cut(s) 49, 171, 181, 237, 281, 356, 539, 566
KpnI GGTACC 1 cut(s) 549
Kzo9I GATC 3 cut(s) 87, 162, 434
LmnI GCTCC 2 cut(s) 263, 564
Lsp1109I GCAGC 2 cut(s) 53, 595
LweI GCATC 2 cut(s) 246, 602
MaeI CTAG 1 cut(s) 422
MaeII ACGT 2 cut(s) 15, 521
MalI GATC 3 cut(s) 89, 164, 436
MboI GATC 3 cut(s) 87, 162, 434
MboII GAAGA 2 cut(s) 10, 203
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 1 cut(s) 87
MluCI AATT 2 cut(s) 115, 201
MnlI CCTC 7 cut(s) 31, 32, 123, 158, 364, 536, 594
Mph1103I ATGCAT 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 242
MspI CCGG 4 cut(s) 25, 35, 97, 575
MspR9I CCNGG 4 cut(s) 26, 97, 527, 576
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 1 cut(s) 580
NciI CCSGG 3 cut(s) 26, 97, 576
NcoI CCATGG 2 cut(s) 45, 562
NdeII GATC 3 cut(s) 87, 162, 434
NlaIII CATG 8 cut(s) 49, 171, 181, 237, 281, 356, 539, 566
NlaIV GGNNCC 3 cut(s) 30, 82, 547
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 2 cut(s) 237, 356
NspV TTCGAA 1 cut(s) 183
PaeI GCATGC 1 cut(s) 356
PagI TCATGA 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 3 cut(s) 318, 389, 413
PfoI TCCNGGA 1 cut(s) 24
PkrI GCNGC 3 cut(s) 43, 71, 585
PpuMI RGGWCCY 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 3 cut(s) 30, 82, 547
PspPI GGNCC 3 cut(s) 339, 572, 577
PspPPI RGGWCCY 1 cut(s) 339
PstI CTGCAG 1 cut(s) 585
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 2 cut(s) 134, 547
RsaNI GTAC 2 cut(s) 133, 546
RseI CAYNNNNRTG 1 cut(s) 242
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 3 cut(s) 42, 70, 584
Sau3AI GATC 3 cut(s) 87, 162, 434
Sau96I GGNCC 3 cut(s) 339, 572, 577
ScrFI CCNGG 4 cut(s) 26, 97, 527, 576
SetI ASST 8 cut(s) 18, 66, 134, 344, 494, 524, 547, 561
SfaNI GCATC 2 cut(s) 246, 602
SfcI CTRYAG 1 cut(s) 581
SfuI TTCGAA 1 cut(s) 183
SinI GGWCC 1 cut(s) 339
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SphI GCATGC 1 cut(s) 356
Sse9I AATT 2 cut(s) 115, 201
SsiI CCGC 4 cut(s) 11, 32, 70, 145
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 4 cut(s) 24, 95, 525, 574
StyI CCWWGG 2 cut(s) 45, 562
TaaI ACNGT 2 cut(s) 218, 334
TaiI ACGT 2 cut(s) 18, 524
TaqI TCGA 1 cut(s) 183
TasI AATT 2 cut(s) 115, 201
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 3 cut(s) 318, 389, 413
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 221
TseI GCWGC 2 cut(s) 41, 583
TspDTI ATGAA 1 cut(s) 266
TspRI CASTG 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 339
XagI CCTNNNNNAGG 1 cut(s) 266
XapI RAATTY 1 cut(s) 115
XbaI TCTAGA 1 cut(s) 421
XceI RCATGY 2 cut(s) 237, 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.