RLG00000021302

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000643
Physical Location & Seq
Forward (+)
73999939 .. 74003362
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021302

Sequence Viewer

Length: 753 bp
ATGTTCTCCTTGGAGCTGTCAAGCATTGTCTCAGCATGCTACTGTAGCCGTGATGGTGGTAGACATTCCCGTAGGAGTATGAGTCGAAGCCGCAGTAGAAGCAGGAGTAGAAGTCAGAGCTTGCAAGCCAGCACTGCTCCTCATGTAAACCCGCCGAGAGATGTAAACAAGGATAGAACATCTGTATCTAGCAATCTGGCAAAACTTAGGGATCTTTTTAGTGATCGAGGGGCAAAAGCACGAGTCTTAGCCCGAAATCGAGGAAGACTGCGTCGAAAATCGGAGCTCCGAAACAGGCCGCAACTACTATTGGGGCTTTCTAAGAGGCCGGTGAAGAAGGAAGACAAGGTTCTTGGTTCAATTGAGCCCAAGAAGCTTTTCAAAGAAGGGGAGAAGTCTGTGCCTAACAAGAAGCCAGTCAAGGCTGGGAGGGTTGTGGCAAGCAGGTACAATCAGATTGTTAATTCTTCGGCTGCTGCTGCGGATGGCCGGAAAAGGATGAAGAAGAGGTGGGAGATTCCTAGTGAGGTGGTGGTGTTCCAGGGTGTGGAGGAGGATGGTGTTGCTGAAATGGGTGCTGTGAATGATGTGCTGCTGAAGATTAGGACTGTTCGATGTGTTAATGACACGCCGAGGGGGTCAGAGACGGTGAAAAGGGTGGCGGAATTGGTTGAGATGAACAACTGTTTTAGCAACGGTGATGAGTTCTGCCAGGAATTGAGTTTTGCAGACGAGGAAGATGGTGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.99

Weight (kDa)

10.08

Isoelectric Point (pI)

63.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 435
AccB7I CCANNNNNTGG 1 cut(s) 547
AccI GTMKAC 1 cut(s) 61
AciI CCGC 5 cut(s) 91, 152, 299, 482, 662
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 487
AcuI CTGAAG 1 cut(s) 617
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 2 cut(s) 360, 382
AjnI CCWGG 2 cut(s) 540, 711
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 16, 120, 286, 376
AluI AGCT 4 cut(s) 16, 120, 286, 376
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 2 cut(s) 34, 638
AlwI GGATC 1 cut(s) 219
AoxI GGCC 3 cut(s) 296, 326, 487
ApeKI GCWGC 4 cut(s) 473, 476, 479, 592
Asp700I GAANNNNTTC 1 cut(s) 377
AsuHPI GGTGA 3 cut(s) 343, 661, 710
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BanII GRGCYC 2 cut(s) 288, 369
BauI CACGAG 1 cut(s) 240
BbsI GAAGAC 2 cut(s) 271, 348
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 460, 463, 466, 579
BccI CCATC 4 cut(s) 47, 479, 551, 734
BceAI ACGGC 1 cut(s) 33
BciT130I CCWGG 2 cut(s) 542, 713
BcoDI GTCTC 2 cut(s) 34, 638
BfaI CTAG 2 cut(s) 189, 522
BfmI CTRYAG 1 cut(s) 43
BfuAI ACCTGC 1 cut(s) 435
BisI GCNGC 6 cut(s) 91, 299, 474, 477, 480, 593
BlsI GCNGC 6 cut(s) 92, 300, 475, 478, 481, 594
Bme1390I CCNGG 2 cut(s) 542, 713
BmrFI CCNGG 2 cut(s) 542, 713
BpiI GAAGAC 2 cut(s) 271, 348
BsaJI CCNNGG 3 cut(s) 9, 541, 632
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 416
BseBI CCWGG 2 cut(s) 542, 713
BseDI CCNNGG 3 cut(s) 9, 541, 632
BseGI GGATG 3 cut(s) 490, 504, 562
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 416
BseRI GAGGAG 2 cut(s) 129, 566
BseXI GCAGC 4 cut(s) 460, 463, 466, 579
BseYI CCCAGC 1 cut(s) 425
BshFI GGCC 3 cut(s) 298, 328, 489
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 2 cut(s) 329, 490
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 2 cut(s) 34, 638
BsmBI CGTCTC 1 cut(s) 638
BsnI GGCC 3 cut(s) 298, 328, 489
Bsp1286I GDGCHC 2 cut(s) 288, 369
Bsp143I GATC 2 cut(s) 211, 223
BspACI CCGC 5 cut(s) 91, 152, 299, 482, 662
BspANI GGCC 3 cut(s) 298, 328, 489
BspCNI CTCAG 1 cut(s) 44
BspMI ACCTGC 1 cut(s) 435
BspPI GGATC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 328
BsrI ACTGG 1 cut(s) 416
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 3 cut(s) 9, 541, 632
BssMI GATC 2 cut(s) 211, 223
BssSI CACGAG 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 240
Bst2UI CCWGG 2 cut(s) 542, 713
Bst4CI ACNGT 5 cut(s) 44, 610, 649, 686, 698
Bst6I CTCTTC 1 cut(s) 500
BstC8I GCNNGC 5 cut(s) 37, 122, 126, 130, 442
BstDEI CTNAG 4 cut(s) 31, 206, 247, 321
BstF5I GGATG 3 cut(s) 490, 504, 562
BstKTI GATC 2 cut(s) 214, 226
BstMAI GTCTC 2 cut(s) 34, 638
BstMBI GATC 2 cut(s) 211, 223
BstMWI GCNNNNNNNGC 5 cut(s) 45, 99, 134, 373, 479
BstNI CCWGG 2 cut(s) 542, 713
BstNSI RCATGY 1 cut(s) 39
BstSCI CCNGG 2 cut(s) 540, 711
BstSFI CTRYAG 1 cut(s) 43
BstV1I GCAGC 4 cut(s) 460, 463, 466, 579
BstV2I GAAGAC 2 cut(s) 271, 348
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 3 cut(s) 298, 328, 489
BtsCI GGATG 3 cut(s) 490, 504, 562
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 132
BveI ACCTGC 1 cut(s) 435
Cac8I GCNNGC 5 cut(s) 37, 122, 126, 130, 442
Cfr10I RCCGGY 1 cut(s) 328
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 448
CviAII CATG 2 cut(s) 36, 143
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 4 cut(s) 31, 206, 247, 321
DpnI GATC 2 cut(s) 213, 225
DpnII GATC 2 cut(s) 211, 223
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 500
EarI CTCTTC 1 cut(s) 500
EciI GGCGGA 1 cut(s) 677
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 2 cut(s) 288, 369
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 540, 711
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 2 cut(s) 288, 369
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 638
FaeI CATG 2 cut(s) 39, 146
FaiI YATR 3 cut(s) 37, 80, 144
FatI CATG 2 cut(s) 35, 142
FauI CCCGC 1 cut(s) 159
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 6 cut(s) 91, 299, 474, 477, 480, 593
FokI GGATG 3 cut(s) 497, 511, 569
FriOI GRGCYC 2 cut(s) 288, 369
Fsp4HI GCNGC 6 cut(s) 91, 299, 474, 477, 480, 593
FspBI CTAG 2 cut(s) 189, 522
GluI GCNGC 6 cut(s) 91, 299, 474, 477, 480, 593
GsaI CCCAGC 1 cut(s) 429
HaeIII GGCC 3 cut(s) 298, 328, 489
HapII CCGG 2 cut(s) 329, 490
HgaI GACGC 1 cut(s) 260
Hin1II CATG 2 cut(s) 39, 146
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 3 cut(s) 82, 243, 517
HpaII CCGG 2 cut(s) 329, 490
HphI GGTGA 3 cut(s) 343, 661, 710
Hpy166II GTNNAC 3 cut(s) 62, 148, 166
Hpy188I TCNGA 5 cut(s) 117, 283, 290, 456, 643
Hpy8I GTNNAC 3 cut(s) 62, 148, 166
Hpy99I CGWCG 1 cut(s) 276
HpyAV CCTTC 2 cut(s) 331, 380
HpyCH4III ACNGT 5 cut(s) 44, 610, 649, 686, 698
HpyCH4V TGCA 2 cut(s) 124, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 99, 134, 373, 479
HpyF3I CTNAG 4 cut(s) 31, 206, 247, 321
Hsp92II CATG 2 cut(s) 39, 146
Kzo9I GATC 2 cut(s) 211, 223
LmnI GCTCC 4 cut(s) 13, 142, 283, 291
Lsp1109I GCAGC 4 cut(s) 460, 463, 466, 579
MaeI CTAG 2 cut(s) 189, 522
MalI GATC 2 cut(s) 213, 225
MboI GATC 2 cut(s) 211, 223
MboII GAAGA 8 cut(s) 276, 346, 353, 459, 514, 517, 610, 749
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 2 cut(s) 288, 369
MluCI AATT 4 cut(s) 360, 463, 665, 716
MlyI GAGTC 2 cut(s) 91, 252
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 2 cut(s) 462, 621
MspI CCGG 2 cut(s) 329, 490
MspR9I CCNGG 2 cut(s) 542, 713
MunI CAATTG 1 cut(s) 360
MvaI CCWGG 2 cut(s) 542, 713
MwoI GCNNNNNNNGC 5 cut(s) 45, 99, 134, 373, 479
NdeII GATC 2 cut(s) 211, 223
NlaIII CATG 2 cut(s) 39, 146
NmeAIII GCCGAG 2 cut(s) 180, 657
NspI RCATGY 1 cut(s) 39
PaeI GCATGC 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 517
PflFI GACNNNGTC 1 cut(s) 270
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 6 cut(s) 92, 300, 475, 478, 481, 594
PleI GAGTC 2 cut(s) 90, 251
PpsI GAGTC 2 cut(s) 90, 251
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 2 cut(s) 540, 711
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 2 cut(s) 540, 711
PsuI RGATCY 1 cut(s) 211
PsyI GACNNNGTC 1 cut(s) 270
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 462, 621
SatI GCNGC 6 cut(s) 91, 299, 474, 477, 480, 593
Sau3AI GATC 2 cut(s) 211, 223
SchI GAGTC 2 cut(s) 91, 252
ScrFI CCNGG 2 cut(s) 542, 713
SduI GDGCHC 2 cut(s) 288, 369
SetI ASST 8 cut(s) 18, 122, 288, 351, 378, 449, 512, 531
SfcI CTRYAG 1 cut(s) 43
SphI GCATGC 1 cut(s) 39
Sse9I AATT 4 cut(s) 360, 463, 665, 716
SsiI CCGC 5 cut(s) 91, 152, 299, 482, 662
SspMI CTAG 2 cut(s) 189, 522
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 540, 711
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 5 cut(s) 44, 610, 649, 686, 698
TaqI TCGA 5 cut(s) 85, 226, 259, 274, 613
TasI AATT 4 cut(s) 360, 463, 665, 716
TauI GCSGC 2 cut(s) 93, 301
TfiI GAWTC 1 cut(s) 517
Tru1I TTAA 2 cut(s) 462, 621
Tru9I TTAA 2 cut(s) 462, 621
TscAI CASTG 1 cut(s) 139
TseI GCWGC 4 cut(s) 473, 476, 479, 592
TspDTI ATGAA 2 cut(s) 515, 692
TspRI CASTG 1 cut(s) 139
Tth111I GACNNNGTC 1 cut(s) 270
Van91I CCANNNNNTGG 1 cut(s) 547
XceI RCATGY 1 cut(s) 39
XmiI GTMKAC 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 2 cut(s) 189, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.