RLG00000029192

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000643
Physical Location & Seq
Forward (+)
34251840 .. 34256814
4975 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029192

Sequence Viewer

Length: 636 bp
ATGATGAAAACAAAGTTATGGTTGATATCCCAGCATCTCAAATGCCACTTGAGTCCCTCTCAACTGCCGGATGATGATAAGGAAGATGGGAGCCGGTGTGAGAAGAAGCGCGTGTCATTGGTGGGGAAGCCACGTGGCATTGGGAGGGAGGGGTCAAGGAGTGTTGGACCGGAGAGTAGGATGAAGAAGAGGTGGGAAATTCCTAGTGAGGTGGTGGTGTTCCAGGGTTTGGAGGAGGACGGTGTTGCTGAAATGGGTGATGTGCTGCCGAAGATTAGGACCAAGAGGTGGATGGTGAAGAAGAATAAGTGGGAGCATGGAGGATTTGGCATGATTTTTCTGAGCTGGAACAAAGGCTTACAGAACAATCAAAGATACTCTCAAGTCTTCAACAACAGGTTGATGATTTGGCATCAGCATGCTTACAACCCAGATGAGGAGTATGCAGAGGTAGAATCCAGATTGCGTTCACATTTGGAATCTTTTCTAGAAACTGCAAGATCATTCAATATGATTTACACCAAGTTCTTGTGGAACTTGAGGAGTCTTAGAGACTCACATGCTTCTCTTGCTGTTGGATCATCTGACTCAGTTGCTGGTGAGCCCTCCTGTCACAAGAATATTCTCAGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

24.47

Weight (kDa)

9.46

Isoelectric Point (pI)

70.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_NMP1 PF06694 138 - 175 5.6e-13 Plant nuclear matrix protein 1 (NMP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 229, 288
AccII CGCG 1 cut(s) 111
AclWI GGATC 1 cut(s) 586
AcsI RAATTY 1 cut(s) 198
AcvI CACGTG 1 cut(s) 134
AfiI CCNNNNNNNGG 3 cut(s) 229, 288, 436
AgsI TTSAA 2 cut(s) 391, 508
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 1 cut(s) 345
AluI AGCT 1 cut(s) 345
Alw26I GTCTC 1 cut(s) 546
AlwI GGATC 1 cut(s) 586
AlwNI CAGNNNCTG 1 cut(s) 596
ApeKI GCWGC 1 cut(s) 265
ApoI RAATTY 1 cut(s) 198
Asp700I GAANNNNTTC 1 cut(s) 483
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 167, 279
AsuHPI GGTGA 3 cut(s) 269, 307, 611
AvaII GGWCC 2 cut(s) 167, 279
BanII GRGCYC 1 cut(s) 606
BbrPI CACGTG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 252
BccI CCATC 2 cut(s) 80, 286
BciT130I CCWGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 546
BfaI CTAG 2 cut(s) 204, 488
BisI GCNGC 1 cut(s) 266
BlsI GCNGC 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 2 cut(s) 167, 279
BmgT120I GGNCC 2 cut(s) 167, 279
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 43, 421
BpiI GAAGAC 1 cut(s) 379
BplI GAGNNNNNCTC 2 cut(s) 43, 75
BpuEI CTTGAG 3 cut(s) 70, 366, 559
BsaAI YACGTR 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 223
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 288, 436
Bse118I RCCGGY 1 cut(s) 93
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 3 cut(s) 76, 186, 297
BseLI CCNNNNNNNGG 3 cut(s) 229, 288, 436
BseMII CTCAG 2 cut(s) 332, 603
BseRI GAGGAG 3 cut(s) 248, 452, 556
BseXI GCAGC 1 cut(s) 252
BseYI CCCAGC 1 cut(s) 30
Bsh1236I CGCG 1 cut(s) 111
BsiSI CCGG 3 cut(s) 68, 94, 170
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 3 cut(s) 229, 288, 436
BsmAI GTCTC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 606
Bsp143I GATC 2 cut(s) 500, 578
BspCNI CTCAG 2 cut(s) 333, 602
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 92
BspPI GGATC 1 cut(s) 586
BsrFI RCCGGY 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 2 cut(s) 500, 578
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 182
BstBAI YACGTR 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 4 cut(s) 341, 548, 589, 626
BstF5I GGATG 3 cut(s) 76, 186, 297
BstFNI CGCG 1 cut(s) 111
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 2 cut(s) 503, 581
BstMAI GTCTC 1 cut(s) 546
BstMBI GATC 2 cut(s) 500, 578
BstMWI GCNNNNNNNGC 1 cut(s) 569
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 2 cut(s) 422, 563
BstSCI CCNGG 1 cut(s) 222
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 379
BtsCI GGATG 3 cut(s) 76, 186, 297
Cac8I GCNNGC 1 cut(s) 420
CaiI CAGNNNCTG 1 cut(s) 596
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 167, 279
CviAII CATG 4 cut(s) 317, 331, 419, 560
CviJI RGCY 5 cut(s) 93, 130, 345, 357, 604
CviKI_1 RGCY 5 cut(s) 93, 130, 345, 357, 604
DdeI CTNAG 4 cut(s) 341, 548, 589, 626
DpnI GATC 2 cut(s) 502, 580
DpnII GATC 2 cut(s) 500, 578
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 606
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 167, 279
Eco72I CACGTG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 222
EcoRV GATATC 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 606
FaeI CATG 4 cut(s) 320, 334, 422, 563
FaiI YATR 7 cut(s) 19, 318, 332, 420, 444, 512, 561
FaqI GGGAC 1 cut(s) 39
FatI CATG 4 cut(s) 316, 330, 418, 559
Fnu4HI GCNGC 1 cut(s) 266
FokI GGATG 3 cut(s) 83, 193, 304
FriOI GRGCYC 1 cut(s) 606
Fsp4HI GCNGC 1 cut(s) 266
FspBI CTAG 2 cut(s) 204, 488
GlaI GCGC 1 cut(s) 110
GluI GCNGC 1 cut(s) 266
GsaI CCCAGC 1 cut(s) 34
HapII CCGG 3 cut(s) 68, 94, 170
HhaI GCGC 1 cut(s) 111
Hin1II CATG 4 cut(s) 320, 334, 422, 563
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HinfI GANTC 6 cut(s) 52, 455, 479, 544, 554, 587
HpaII CCGG 3 cut(s) 68, 94, 170
HphI GGTGA 3 cut(s) 269, 307, 611
Hpy166II GTNNAC 1 cut(s) 470
Hpy188I TCNGA 3 cut(s) 342, 586, 629
Hpy188III TCNNGA 2 cut(s) 459, 488
Hpy8I GTNNAC 1 cut(s) 470
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 2 cut(s) 446, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 569
HpyF3I CTNAG 4 cut(s) 341, 548, 589, 626
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 4 cut(s) 320, 334, 422, 563
HspAI GCGC 1 cut(s) 109
Kzo9I GATC 2 cut(s) 500, 578
LmnI GCTCC 2 cut(s) 90, 313
Lsp1109I GCAGC 1 cut(s) 252
LweI GCATC 2 cut(s) 43, 421
MaeI CTAG 2 cut(s) 204, 488
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 1 cut(s) 611
MalI GATC 2 cut(s) 502, 580
MboI GATC 2 cut(s) 500, 578
MboII GAAGA 8 cut(s) 95, 115, 196, 199, 283, 310, 313, 379
MhlI GDGCHC 1 cut(s) 606
MluCI AATT 1 cut(s) 198
MlyI GAGTC 4 cut(s) 61, 548, 553, 581
MmeI TCCRAC 2 cut(s) 145, 556
MroXI GAANNNNTTC 1 cut(s) 483
MslI CAYNNNNRTG 1 cut(s) 417
MspI CCGG 3 cut(s) 68, 94, 170
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 569
NdeII GATC 2 cut(s) 500, 578
NlaIII CATG 4 cut(s) 320, 334, 422, 563
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 611
NspI RCATGY 2 cut(s) 422, 563
PaeI GCATGC 1 cut(s) 422
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 455, 479
PflMI CCANNNNNTGG 2 cut(s) 229, 288
PkrI GCNGC 1 cut(s) 267
PleI GAGTC 4 cut(s) 60, 548, 552, 581
PmaCI CACGTG 1 cut(s) 134
PmlI CACGTG 1 cut(s) 134
PpsI GAGTC 4 cut(s) 60, 548, 552, 581
Ppu21I YACGTR 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 222
PspCI CACGTG 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 167, 279
PstNI CAGNNNCTG 1 cut(s) 596
RseI CAYNNNNRTG 1 cut(s) 417
SatI GCNGC 1 cut(s) 266
Sau3AI GATC 2 cut(s) 500, 578
Sau96I GGNCC 2 cut(s) 167, 279
SchI GAGTC 4 cut(s) 61, 548, 553, 581
ScrFI CCNGG 1 cut(s) 224
SduI GDGCHC 1 cut(s) 606
SetI ASST 7 cut(s) 136, 194, 213, 290, 347, 401, 453
SfaNI GCATC 2 cut(s) 43, 421
SinI GGWCC 2 cut(s) 167, 279
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 3 cut(s) 49, 381, 538
SmoI CTYRAG 3 cut(s) 49, 381, 538
SphI GCATGC 1 cut(s) 422
Sse9I AATT 1 cut(s) 198
SspI AATATT 1 cut(s) 622
SspMI CTAG 2 cut(s) 204, 488
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 136
TasI AATT 1 cut(s) 198
TfiI GAWTC 2 cut(s) 455, 479
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 2 cut(s) 20, 197
Van91I CCANNNNNTGG 2 cut(s) 229, 288
VpaK11BI GGWCC 2 cut(s) 167, 279
XapI RAATTY 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 2 cut(s) 422, 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 2 cut(s) 204, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.