MD09G1096400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000643
Physical Location & Seq
Reverse (-)
6956276 .. 6957697
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1096400.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGAGTGTTGTTGAATACCCAGACGCCATTAACGCACCAGACCTTCAGGTTTGGAACAACGCCGCCTTCGACAACGAAGACTCCGAAAGCGGCTCCTCCGTCGTAAAAGCTTCCTGGTCCGAGCTACTGCAACCCCTTTCGCTGATTCGTTCTTCCGAGTCGTTTGATTCGGGTTGCAGCAAAGAGAACCTGAGCCCAGAGATTCTGAAAACCCCTGTCTGTGTCAAATCTTTGGTGCCCTTCAAGCCGCTGGACACAAATACGAATCTTGAGCCCATCTCGGTGGTCGCGAAGAAGAAGGGTATTGGGGAGGTTGAAGAAAGAGAAGAGAAAGTTCGAGATGAGGGAAAGATCGATGCTGAAATTGAAGCGATTGAGAAGGAGATTAGTCGATTGAATTCGAGACTCGAAGCGCTTAAACTCGAAAAGGCTGGGAGAAACGAGAAGGCGGTAGAGAAGCGCGGAAGGGTTGTGGCGGCGAAGTTCATGGAGCCGAAACAGAGTGTGAAGAATTTGGACGGGTTGAAAAAGATTGAGTCTTTGATGATGAGTGTGAGGCCAAAGGTTAATCGGAGAGGAATGAGTTTGGGGCCCTCTGAGATTATAGCTGGAGCAGAATTTCGGAGGCCGAGTAAGCTCGAGATCACCCCTGTTCAGGAAACCCAGAGTCGCCGGAAATCTTGCTTTTGGAAGCTTCAGGACATTGATAAATTGAGGGCTACAAAAGAGCGGGGCAAGAGTTTGAGTTTGAGCCCGAAATCACGGAAAACTGTGTCCAAGGCTCAAGCTCCTAAACAGGCAGCGACTACTGTTGGAGGGTCTAAGAGACCTGTGAAGAAGGAAGACAAGGTTCTTGCATCAATTGAACCCAAGAAGCTTTTTAAAGACGGAGCCGAGAAGTCTGCGCCCGCCAAGAAAACACCATTCAAGCCTGGGAGGGTTGTGCCTAGCAGGTACAATCAGATAGGGAATTCGGCAGTGAGTGATGGCCGGAAGCGGTCTTGGCCCGAGGATGACAAGGATGACAGCAAGCGGTGTGACAAGAGGCGGGTATCCTTGGCAGGGAAGCCGCGTGGCATTGGCCGTGAAATGTCCAGGAGTCAAGGACCGGAGTGCCGGGTGAAGAAGAGGTGGGAGATTCCAAGTGAGGTAGTGGTGTACCAAGGTGTGGCGGAGGATGGTAAGTCACCTTCAGTTGTTGCTGAGATAGGAGATGTCCTTCCAAAAATTAAGACTGTCCGATGTGGTATTGATACTCCGAGGGGTTCGGGGCCAGCCAAAAGGGTTGCTGAATTGGTTGGGATGAAATCCTACTTTAGCACCAATGGGGAGGTTTGCCAGGGATTGAATTTTGCAGAAGCGGATGCTGAGGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

460

Amino Acids

50.6

Weight (kDa)

9.49

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 942
AccB1I GGYRCC 1 cut(s) 235
AccB7I CCANNNNNTGG 1 cut(s) 1168
AccBSI CCGCTC 1 cut(s) 730
AccII CGCG 3 cut(s) 290, 462, 1072
AcoI YGGCCR 2 cut(s) 988, 1081
AcsI RAATTY 5 cut(s) 397, 511, 617, 970, 1348
AcuI CTGAAG 3 cut(s) 29, 680, 1176
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 2 cut(s) 956, 1160
AfeI AGCGCT 1 cut(s) 414
AfiI CCNNNNNNNGG 3 cut(s) 655, 1062, 1168
AgsI TTSAA 9 cut(s) 14, 244, 317, 368, 397, 526, 866, 928, 1348
AjnI CCWGG 4 cut(s) 113, 931, 1094, 1338
AluBI AGCT 7 cut(s) 110, 124, 608, 637, 694, 788, 877
AluI AGCT 7 cut(s) 110, 124, 608, 637, 694, 788, 877
Alw26I GTCTC 2 cut(s) 397, 820
Ama87I CYCGRG 2 cut(s) 638, 1007
Aor51HI AGCGCT 1 cut(s) 414
AoxI GGCC 7 cut(s) 557, 590, 626, 988, 1004, 1081, 1271
ApaI GGGCCC 1 cut(s) 594
ApeKI GCWGC 2 cut(s) 177, 800
ApoI RAATTY 5 cut(s) 397, 511, 617, 970, 1348
AspLEI GCGC 3 cut(s) 415, 462, 907
AspS9I GGNCC 6 cut(s) 117, 590, 591, 1005, 1106, 1271
AsuC2I CCSGG 1 cut(s) 1118
AsuHPI GGTGA 3 cut(s) 637, 1132, 1179
AvaI CYCGRG 2 cut(s) 638, 1007
AvaII GGWCC 2 cut(s) 117, 1106
BaeGI GKGCMC 2 cut(s) 240, 594
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 4 cut(s) 197, 276, 594, 755
BarI GAAGNNNNNNTAC 2 cut(s) 1174, 1206
BbsI GAAGAC 2 cut(s) 84, 849
BbvCI CCTCAGC 1 cut(s) 1368
BbvI GCAGC 2 cut(s) 189, 812
BccI CCATC 3 cut(s) 284, 980, 1172
BceAI ACGGC 1 cut(s) 1068
BcgI CGANNNNNNTGC 2 cut(s) 884, 918
BciT130I CCWGG 4 cut(s) 115, 933, 1096, 1340
BciVI GTATCC 1 cut(s) 1063
BcnI CCSGG 1 cut(s) 1118
BcoDI GTCTC 2 cut(s) 397, 820
BfaI CTAG 1 cut(s) 948
BfoI RGCGCY 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 942
BfuI GTATCC 1 cut(s) 1063
BisI GCNGC 7 cut(s) 63, 91, 178, 248, 477, 801, 1070
BlsI GCNGC 7 cut(s) 64, 92, 179, 249, 478, 802, 1071
Bme1390I CCNGG 5 cut(s) 115, 933, 1096, 1118, 1340
Bme18I GGWCC 2 cut(s) 117, 1106
BmeT110I CYCGRG 2 cut(s) 638, 1007
BmgT120I GGNCC 6 cut(s) 117, 590, 591, 1005, 1106, 1271
BmiI GGNNCC 7 cut(s) 94, 237, 492, 591, 592, 892, 1272
BmrFI CCNGG 5 cut(s) 115, 933, 1096, 1118, 1340
BmsI GCATC 3 cut(s) 346, 866, 1354
BpiI GAAGAC 2 cut(s) 84, 849
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 2 cut(s) 191, 1368
BpuEI CTTGAG 2 cut(s) 290, 768
BpuMI CCSGG 1 cut(s) 1118
Bsa29I ATCGAT 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 820
BsaJI CCNNGG 7 cut(s) 777, 932, 1008, 1056, 1162, 1259, 1339
BsaWI WCCGGW 1 cut(s) 1108
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 616, 646
Bsc4I CCNNNNNNNGG 3 cut(s) 655, 1062, 1168
BseBI CCWGG 4 cut(s) 115, 933, 1096, 1340
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 7 cut(s) 777, 932, 1008, 1056, 1162, 1259, 1339
BseGI GGATG 5 cut(s) 1018, 1027, 1183, 1308, 1369
BseLI CCNNNNNNNGG 3 cut(s) 655, 1062, 1168
BseMII CTCAG 4 cut(s) 182, 588, 1194, 1359
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 2 cut(s) 240, 594
BseXI GCAGC 2 cut(s) 189, 812
BseYI CCCAGC 1 cut(s) 431
Bsh1236I CGCG 3 cut(s) 290, 462, 1072
BshFI GGCC 7 cut(s) 559, 592, 628, 990, 1006, 1083, 1273
BshNI GGYRCC 1 cut(s) 235
BshVI ATCGAT 1 cut(s) 354
BsiHKCI CYCGRG 2 cut(s) 638, 1007
BsiSI CCGG 4 cut(s) 673, 991, 1109, 1117
BslI CCNNNNNNNGG 3 cut(s) 655, 1062, 1168
BsmAI GTCTC 2 cut(s) 397, 820
BsnI GGCC 7 cut(s) 559, 592, 628, 990, 1006, 1083, 1273
Bso31I GGTCTC 1 cut(s) 820
BsoBI CYCGRG 2 cut(s) 638, 1007
Bsp120I GGGCCC 1 cut(s) 590
Bsp1286I GDGCHC 5 cut(s) 197, 240, 276, 594, 755
Bsp143I GATC 2 cut(s) 351, 642
Bsp68I TCGCGA 1 cut(s) 290
BspANI GGCC 7 cut(s) 559, 592, 628, 990, 1006, 1083, 1273
BspCNI CTCAG 4 cut(s) 183, 589, 1195, 1360
BspDI ATCGAT 1 cut(s) 354
BspFNI CGCG 3 cut(s) 290, 462, 1072
BspLI GGNNCC 7 cut(s) 94, 237, 492, 591, 592, 892, 1272
BspMI ACCTGC 1 cut(s) 942
BspT107I GGYRCC 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 820
BsrBI CCGCTC 1 cut(s) 730
BssECI CCNNGG 7 cut(s) 777, 932, 1008, 1056, 1162, 1259, 1339
BssMI GATC 2 cut(s) 351, 642
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 3 cut(s) 777, 1056, 1162
Bst2UI CCWGG 4 cut(s) 115, 933, 1096, 1340
Bst4CI ACNGT 3 cut(s) 772, 811, 1237
Bst6I CTCTTC 2 cut(s) 321, 1121
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 3 cut(s) 909, 1031, 1275
BstDEI CTNAG 5 cut(s) 191, 597, 822, 1203, 1368
BstF5I GGATG 5 cut(s) 1018, 1027, 1183, 1308, 1369
BstFNI CGCG 3 cut(s) 290, 462, 1072
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 3 cut(s) 415, 462, 907
BstKTI GATC 2 cut(s) 354, 645
BstMAI GTCTC 2 cut(s) 397, 820
BstMBI GATC 2 cut(s) 351, 642
BstMWI GCNNNNNNNGC 4 cut(s) 32, 244, 634, 1003
BstNI CCWGG 4 cut(s) 115, 933, 1096, 1340
BstSCI CCNGG 5 cut(s) 113, 931, 1094, 1116, 1338
BstSLI GKGCMC 2 cut(s) 240, 594
BstUI CGCG 3 cut(s) 290, 462, 1072
BstV1I GCAGC 2 cut(s) 189, 812
BstV2I GAAGAC 2 cut(s) 84, 849
BstXI CCANNNNNNTGG 1 cut(s) 283
Bsu15I ATCGAT 1 cut(s) 354
BsuI GTATCC 1 cut(s) 1063
BsuRI GGCC 7 cut(s) 559, 592, 628, 990, 1006, 1083, 1273
BsuTUI ATCGAT 1 cut(s) 354
BtsCI GGATG 5 cut(s) 1018, 1027, 1183, 1308, 1369
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 1 cut(s) 984
BtuMI TCGCGA 1 cut(s) 290
BveI ACCTGC 1 cut(s) 942
Cac8I GCNNGC 3 cut(s) 909, 1031, 1275
CfoI GCGC 3 cut(s) 415, 462, 907
Cfr13I GGNCC 6 cut(s) 117, 590, 591, 1005, 1106, 1271
ClaI ATCGAT 1 cut(s) 354
CseI GACGC 1 cut(s) 32
Csp6I GTAC 2 cut(s) 955, 1159
CviAII CATG 1 cut(s) 487
CviQI GTAC 2 cut(s) 955, 1159
DdeI CTNAG 5 cut(s) 191, 597, 822, 1203, 1368
DpnI GATC 2 cut(s) 353, 644
DpnII GATC 2 cut(s) 351, 642
DraI TTTAAA 1 cut(s) 883
EaeI YGGCCR 2 cut(s) 988, 1081
Eam1104I CTCTTC 2 cut(s) 321, 1121
EarI CTCTTC 2 cut(s) 321, 1121
EciI GGCGGA 1 cut(s) 1187
Eco130I CCWWGG 3 cut(s) 777, 1056, 1162
Eco24I GRGCYC 4 cut(s) 197, 276, 594, 755
Eco31I GGTCTC 1 cut(s) 820
Eco47I GGWCC 2 cut(s) 117, 1106
Eco47III AGCGCT 1 cut(s) 414
Eco57I CTGAAG 3 cut(s) 29, 680, 1176
Eco88I CYCGRG 2 cut(s) 638, 1007
EcoO109I RGGNCCY 2 cut(s) 590, 591
EcoRI GAATTC 2 cut(s) 397, 970
EcoRII CCWGG 4 cut(s) 113, 931, 1094, 1338
EcoT14I CCWWGG 3 cut(s) 777, 1056, 1162
EcoT38I GRGCYC 4 cut(s) 197, 276, 594, 755
ErhI CCWWGG 3 cut(s) 777, 1056, 1162
FaeI CATG 1 cut(s) 490
FaiI YATR 2 cut(s) 488, 605
FatI CATG 1 cut(s) 486
FauI CCCGC 3 cut(s) 723, 916, 1041
Fnu4HI GCNGC 7 cut(s) 63, 91, 178, 248, 477, 801, 1070
FokI GGATG 5 cut(s) 1025, 1034, 1190, 1315, 1376
FriOI GRGCYC 4 cut(s) 197, 276, 594, 755
Fsp4HI GCNGC 7 cut(s) 63, 91, 178, 248, 477, 801, 1070
FspBI CTAG 1 cut(s) 948
GlaI GCGC 3 cut(s) 414, 461, 906
GluI GCNGC 7 cut(s) 63, 91, 178, 248, 477, 801, 1070
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 7 cut(s) 559, 592, 628, 990, 1006, 1083, 1273
HapII CCGG 4 cut(s) 673, 991, 1109, 1117
HgaI GACGC 1 cut(s) 32
HhaI GCGC 3 cut(s) 415, 462, 907
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 1 cut(s) 490
Hin6I GCGC 3 cut(s) 413, 460, 905
HinP1I GCGC 3 cut(s) 413, 460, 905
HindIII AAGCTT 3 cut(s) 108, 692, 875
HpaII CCGG 4 cut(s) 673, 991, 1109, 1117
HphI GGTGA 3 cut(s) 637, 1132, 1179
Hpy166II GTNNAC 1 cut(s) 1159
Hpy188III TCNNGA 7 cut(s) 269, 289, 338, 402, 640, 656, 698
Hpy8I GTNNAC 1 cut(s) 1159
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 3 cut(s) 772, 811, 1237
HpyCH4V TGCA 4 cut(s) 130, 177, 857, 1355
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 244, 634, 1003
HpyF3I CTNAG 5 cut(s) 191, 597, 822, 1203, 1368
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 1 cut(s) 490
HspAI GCGC 3 cut(s) 413, 460, 905
Kzo9I GATC 2 cut(s) 351, 642
LmnI GCTCC 5 cut(s) 98, 490, 611, 793, 890
Lsp1109I GCAGC 2 cut(s) 189, 812
LweI GCATC 3 cut(s) 346, 866, 1354
MaeI CTAG 1 cut(s) 948
MaeIII GTNAC 2 cut(s) 1037, 1185
MalI GATC 2 cut(s) 353, 644
MbiI CCGCTC 1 cut(s) 730
MboI GATC 2 cut(s) 351, 642
MfeI CAATTG 1 cut(s) 861
MhlI GDGCHC 5 cut(s) 197, 240, 276, 594, 755
MlyI GAGTC 6 cut(s) 74, 167, 399, 545, 676, 1108
MmeI TCCRAC 1 cut(s) 793
MseI TTAA 5 cut(s) 30, 417, 567, 882, 1230
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 4 cut(s) 673, 991, 1109, 1117
MspR9I CCNGG 5 cut(s) 115, 933, 1096, 1118, 1340
MunI CAATTG 1 cut(s) 861
MvaI CCWGG 4 cut(s) 115, 933, 1096, 1340
MvnI CGCG 3 cut(s) 290, 462, 1072
MwoI GCNNNNNNNGC 4 cut(s) 32, 244, 634, 1003
NciI CCSGG 1 cut(s) 1118
NdeII GATC 2 cut(s) 351, 642
NlaIII CATG 1 cut(s) 490
NlaIV GGNNCC 7 cut(s) 94, 237, 492, 591, 592, 892, 1272
NmeAIII GCCGAG 2 cut(s) 654, 919
NmuCI GTSAC 2 cut(s) 1037, 1185
NruI TCGCGA 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 638
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 81, 287
PfeI GAWTC 5 cut(s) 145, 167, 202, 265, 1138
PflMI CCANNNNNTGG 1 cut(s) 1168
PfoI TCCNGGA 1 cut(s) 1094
PkrI GCNGC 7 cut(s) 64, 92, 179, 249, 478, 802, 1071
PleI GAGTC 6 cut(s) 74, 166, 399, 544, 675, 1107
PpsI GAGTC 6 cut(s) 74, 166, 399, 544, 675, 1107
Psp6I CCWGG 4 cut(s) 113, 931, 1094, 1338
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 4 cut(s) 113, 931, 1094, 1338
PspN4I GGNNCC 7 cut(s) 94, 237, 492, 591, 592, 892, 1272
PspOMI GGGCCC 1 cut(s) 590
PspPI GGNCC 6 cut(s) 117, 590, 591, 1005, 1106, 1271
RruI TCGCGA 1 cut(s) 290
RsaI GTAC 2 cut(s) 956, 1160
RsaNI GTAC 2 cut(s) 955, 1159
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 5 cut(s) 30, 417, 567, 882, 1230
SatI GCNGC 7 cut(s) 63, 91, 178, 248, 477, 801, 1070
Sau3AI GATC 2 cut(s) 351, 642
Sau96I GGNCC 6 cut(s) 117, 590, 591, 1005, 1106, 1271
SchI GAGTC 6 cut(s) 74, 167, 399, 545, 676, 1108
ScrFI CCNGG 5 cut(s) 115, 933, 1096, 1118, 1340
SduI GDGCHC 5 cut(s) 197, 240, 276, 594, 755
SfaNI GCATC 3 cut(s) 346, 866, 1354
Sfr274I CTCGAG 1 cut(s) 638
SinI GGWCC 2 cut(s) 117, 1106
SlaI CTCGAG 1 cut(s) 638
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 3 cut(s) 269, 638, 783
SmoI CTYRAG 3 cut(s) 269, 638, 783
SspMI CTAG 1 cut(s) 948
StyD4I CCNGG 5 cut(s) 113, 931, 1094, 1116, 1338
StyI CCWWGG 3 cut(s) 777, 1056, 1162
TaaI ACNGT 3 cut(s) 772, 811, 1237
TaqI TCGA 8 cut(s) 69, 337, 354, 391, 401, 408, 423, 639
TauI GCSGC 5 cut(s) 65, 93, 250, 479, 1072
TfiI GAWTC 5 cut(s) 145, 167, 202, 265, 1138
Tru1I TTAA 5 cut(s) 30, 417, 567, 882, 1230
Tru9I TTAA 5 cut(s) 30, 417, 567, 882, 1230
TscAI CASTG 1 cut(s) 984
TseFI GTSAC 2 cut(s) 1037, 1185
TseI GCWGC 2 cut(s) 177, 800
Tsp45I GTSAC 2 cut(s) 1037, 1185
TspDTI ATGAA 2 cut(s) 475, 1319
TspGWI ACGGA 3 cut(s) 88, 778, 903
TspRI CASTG 1 cut(s) 984
Van91I CCANNNNNTGG 1 cut(s) 1168
VpaK11BI GGWCC 2 cut(s) 117, 1106
XapI RAATTY 5 cut(s) 397, 511, 617, 970, 1348
XhoI CTCGAG 1 cut(s) 638
XspI CTAG 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.