MD17G1084500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000643
Physical Location & Seq
Reverse (-)
6949526 .. 6950899
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1084500.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGAGTGTAGTTGAATGCCAAGACGCCATTAACGCACCAGACCTTCAGGTGTGGAACAACGCCGCCTTCGACAACGAAGACTCCGAAGGTGGCTCCTCCGTCGTGAAGGCTTCCTGGTCCGATATACTCCAACCCCTTTCGCTGAATTGCTCTTCCGAGTCGTTTGAATCGGGTTGCAGCAAAGAGAATCTGAGCCCCGCGATTCTGAAAACCCCTGTCTGCGTCAAATCTTCGGTGCCCTTCAAGCCGCTGGACACAAATACGAATCTTGATCCCTTCTCGGCGGTTGCGAAGAAGAAGGGTCTTGCAGAGGTTAAAGAAGGAGAAGAGAGAGTTCGAGATGAGGGGAAGATCGATGCGGAGATTGAAGAGATTGAGAAGGAGATTAGTAGATTGAATTCGAGGCTGGAAGCGCTTAAACTTGAAAAGGCTGAGAGAAACGAGAAGGCGGTAGAGAAGCGTGGAAGGGTTGTGGCGGCGAAGTTCATGGAGCAGAAACAAGGTGTGAAGAATTTGGATGGGTTGAAAAAGATTGAGTCTATGATGATGAGCGTGAGGTCGAAGGATAATCGGAGAGGAATGAGTTTGGGGCCGTCTGAGATTATAGCTGGAGCCGGATTTCAGAGGCCGAGTAAGTTCGAGATCACCCCTGTTCAGGCAACGCAGAGTCGCCGGAAATCGTGCTTTTGGAAGCTTCAGGACATCGATGAATTGAGGGCTACGAAAGAGAGGGGCAAGAGTTTGAGTTTGAGTCCGAAATCGAGGAAAACTGTGTCCAAGGTTCAAGCTCCGAAACAGGCTGCAACTACTGTTGGAGGGTCCAAAAGGCCTGTGAAGAAGCAAGACAAGGTTCTTGCATCAATTGGACCCAAGAAGCTTTTTAAAGACGGAGCCGAGAAGTCTGTGCCTGCTAAGAAAACACCATTCAAGCCTGGGAGGGTTGTGCCTAGTAGGTACAATCAGATTGGGAATTCGGCAGTGAGTGATGGTCGGAAGCGGTCTTGGCCTGAGGATGATAAGGATGACAGTAAGAGAGGTGACAAGAGGCGGGTCTCCTTGGTTGGAAAGCCGCATGGCATTGCCCGTGAGACATCCAGGAGTCAAGGACCGGAGTGCCGGGTGAAGAAGAGGTGGGAGATTCCAAGTGAGATAGTGGTGTACCAAGTTGCAGCAGAGGATAACAAGTCACCTTCAGTTGAGATAGGAGATGTGCTGCCAAAAATTAAGACAGTCCGATGTGGTATTGATACTCCGAGGGGTTCGGGGCCAGCCAAAAGGGCTGCTGAATTGGTTGGGATGAAATCCTACTTTAGCACCAATGGGGAGGTTTGCCATGAATTGAGTTTTGCAGAAGAAGGTGCTGAGGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

458

Amino Acids

50.24

Weight (kDa)

9.36

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccII CGCG 1 cut(s) 200
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 3 cut(s) 397, 511, 970
AcuI CTGAAG 3 cut(s) 29, 680, 1176
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 2 cut(s) 956, 1160
AfeI AGCGCT 1 cut(s) 414
AfiI CCNNNNNNNGG 1 cut(s) 655
AgsI TTSAA 9 cut(s) 14, 167, 244, 368, 397, 425, 526, 785, 928
AjnI CCWGG 3 cut(s) 113, 931, 1094
AluBI AGCT 4 cut(s) 608, 694, 788, 877
AluI AGCT 4 cut(s) 608, 694, 788, 877
Alw26I GTCTC 2 cut(s) 1057, 1082
AlwI GGATC 1 cut(s) 266
Aor51HI AGCGCT 1 cut(s) 414
AoxI GGCC 5 cut(s) 590, 626, 827, 1004, 1265
ApeKI GCWGC 5 cut(s) 177, 800, 1169, 1213, 1280
ApoI RAATTY 3 cut(s) 397, 511, 970
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 6 cut(s) 117, 590, 819, 866, 1106, 1265
AsuC2I CCSGG 1 cut(s) 1118
AsuHPI GGTGA 4 cut(s) 637, 1049, 1132, 1179
AvaII GGWCC 4 cut(s) 117, 819, 866, 1106
AxyI CCTNAGG 1 cut(s) 1008
BaeGI GKGCMC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 84
BbvCI CCTCAGC 1 cut(s) 1362
BbvI GCAGC 5 cut(s) 189, 787, 1181, 1200, 1267
BccI CCATC 2 cut(s) 512, 980
BceAI ACGGC 1 cut(s) 577
BciT130I CCWGG 3 cut(s) 115, 933, 1096
BcnI CCSGG 1 cut(s) 1118
BcoDI GTCTC 2 cut(s) 1057, 1082
BfaI CTAG 1 cut(s) 948
BfoI RGCGCY 1 cut(s) 416
BglI GCCNNNNNGGC 1 cut(s) 1277
BisI GCNGC 9 cut(s) 63, 178, 248, 477, 801, 1070, 1170, 1214, 1281
BlsI GCNGC 9 cut(s) 64, 179, 249, 478, 802, 1071, 1171, 1215, 1282
Bme1390I CCNGG 4 cut(s) 115, 933, 1096, 1118
Bme18I GGWCC 4 cut(s) 117, 819, 866, 1106
BmgT120I GGNCC 6 cut(s) 117, 590, 819, 866, 1106, 1265
BmiI GGNNCC 8 cut(s) 94, 237, 591, 613, 820, 868, 892, 1266
BmrFI CCNGG 4 cut(s) 115, 933, 1096, 1118
BmsI GCATC 2 cut(s) 346, 866
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 1362
BpuMI CCSGG 1 cut(s) 1118
Bsa29I ATCGAT 2 cut(s) 354, 705
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 1057
BsaJI CCNNGG 4 cut(s) 777, 932, 1056, 1253
BsaWI WCCGGW 1 cut(s) 1108
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 1 cut(s) 655
Bse21I CCTNAGG 1 cut(s) 1008
Bse3DI GCAATG 1 cut(s) 1077
BseBI CCWGG 3 cut(s) 115, 933, 1096
BseCI ATCGAT 2 cut(s) 354, 705
BseDI CCNNGG 4 cut(s) 777, 932, 1056, 1253
BseGI GGATG 5 cut(s) 523, 1018, 1027, 1091, 1302
BseLI CCNNNNNNNGG 1 cut(s) 655
BseMI GCAATG 1 cut(s) 1077
BseMII CTCAG 5 cut(s) 182, 423, 588, 999, 1353
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 5 cut(s) 189, 787, 1181, 1200, 1267
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 5 cut(s) 592, 628, 829, 1006, 1267
BshNI GGYRCC 1 cut(s) 235
BshVI ATCGAT 2 cut(s) 354, 705
BsiSI CCGG 4 cut(s) 615, 673, 1109, 1117
BslI CCNNNNNNNGG 1 cut(s) 655
BsmAI GTCTC 2 cut(s) 1057, 1082
BsmI GAATGC 1 cut(s) 20
BsnI GGCC 5 cut(s) 592, 628, 829, 1006, 1267
Bso31I GGTCTC 1 cut(s) 1057
Bsp1286I GDGCHC 2 cut(s) 197, 240
Bsp143I GATC 3 cut(s) 271, 351, 642
BspANI GGCC 5 cut(s) 592, 628, 829, 1006, 1267
BspCNI CTCAG 5 cut(s) 183, 424, 589, 1000, 1354
BspDI ATCGAT 2 cut(s) 354, 705
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 8 cut(s) 94, 237, 591, 613, 820, 868, 892, 1266
BspPI GGATC 1 cut(s) 266
BspQI GCTCTTC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 1077
BssECI CCNNGG 4 cut(s) 777, 932, 1056, 1253
BssMI GATC 3 cut(s) 271, 351, 642
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 2 cut(s) 777, 1056
Bst2UI CCWGG 3 cut(s) 115, 933, 1096
Bst4CI ACNGT 4 cut(s) 772, 811, 1028, 1231
Bst6I CTCTTC 4 cut(s) 157, 321, 363, 1121
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 909, 1269
BstDEI CTNAG 6 cut(s) 191, 432, 597, 912, 1008, 1362
BstF5I GGATG 5 cut(s) 523, 1018, 1027, 1091, 1302
BstFNI CGCG 1 cut(s) 200
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 3 cut(s) 274, 354, 645
BstMAI GTCTC 2 cut(s) 1057, 1082
BstMBI GATC 3 cut(s) 271, 351, 642
BstMWI GCNNNNNNNGC 5 cut(s) 32, 244, 412, 1003, 1277
BstNI CCWGG 3 cut(s) 115, 933, 1096
BstSCI CCNGG 4 cut(s) 113, 931, 1094, 1116
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 5 cut(s) 189, 787, 1181, 1200, 1267
BstV2I GAAGAC 1 cut(s) 84
Bsu15I ATCGAT 2 cut(s) 354, 705
Bsu36I CCTNAGG 1 cut(s) 1008
BsuRI GGCC 5 cut(s) 592, 628, 829, 1006, 1267
BsuTUI ATCGAT 2 cut(s) 354, 705
BtsCI GGATG 5 cut(s) 523, 1018, 1027, 1091, 1302
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 1 cut(s) 984
Cac8I GCNNGC 2 cut(s) 909, 1269
CfoI GCGC 1 cut(s) 415
Cfr13I GGNCC 6 cut(s) 117, 590, 819, 866, 1106, 1265
ClaI ATCGAT 2 cut(s) 354, 705
CseI GACGC 2 cut(s) 32, 211
Csp6I GTAC 2 cut(s) 955, 1159
CviAII CATG 3 cut(s) 487, 1073, 1334
CviQI GTAC 2 cut(s) 955, 1159
DdeI CTNAG 6 cut(s) 191, 432, 597, 912, 1008, 1362
DpnI GATC 3 cut(s) 273, 353, 644
DpnII GATC 3 cut(s) 271, 351, 642
DraI TTTAAA 1 cut(s) 883
Eam1104I CTCTTC 4 cut(s) 157, 321, 363, 1121
EarI CTCTTC 4 cut(s) 157, 321, 363, 1121
Eco130I CCWWGG 2 cut(s) 777, 1056
Eco147I AGGCCT 1 cut(s) 829
Eco24I GRGCYC 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 1057
Eco47I GGWCC 4 cut(s) 117, 819, 866, 1106
Eco47III AGCGCT 1 cut(s) 414
Eco57I CTGAAG 3 cut(s) 29, 680, 1176
Eco81I CCTNAGG 1 cut(s) 1008
EcoRI GAATTC 2 cut(s) 397, 970
EcoRII CCWGG 3 cut(s) 113, 931, 1094
EcoT14I CCWWGG 2 cut(s) 777, 1056
EcoT38I GRGCYC 1 cut(s) 197
ErhI CCWWGG 2 cut(s) 777, 1056
FaeI CATG 3 cut(s) 490, 1076, 1337
FaiI YATR 6 cut(s) 125, 488, 542, 605, 1074, 1335
FatI CATG 3 cut(s) 486, 1072, 1333
FauI CCCGC 2 cut(s) 205, 1041
Fnu4HI GCNGC 9 cut(s) 63, 178, 248, 477, 801, 1070, 1170, 1214, 1281
FokI GGATG 5 cut(s) 530, 1025, 1034, 1078, 1309
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 9 cut(s) 63, 178, 248, 477, 801, 1070, 1170, 1214, 1281
FspBI CTAG 1 cut(s) 948
GlaI GCGC 1 cut(s) 414
GluI GCNGC 9 cut(s) 63, 178, 248, 477, 801, 1070, 1170, 1214, 1281
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 5 cut(s) 592, 628, 829, 1006, 1267
HapII CCGG 4 cut(s) 615, 673, 1109, 1117
HgaI GACGC 2 cut(s) 32, 211
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 3 cut(s) 490, 1076, 1337
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HindIII AAGCTT 2 cut(s) 692, 875
HpaII CCGG 4 cut(s) 615, 673, 1109, 1117
HphI GGTGA 4 cut(s) 637, 1049, 1132, 1179
Hpy166II GTNNAC 1 cut(s) 1159
Hpy188III TCNNGA 5 cut(s) 103, 269, 338, 640, 698
Hpy8I GTNNAC 1 cut(s) 1159
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 4 cut(s) 772, 811, 1028, 1231
HpyCH4V TGCA 6 cut(s) 177, 308, 803, 857, 1169, 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 244, 412, 1003, 1277
HpyF3I CTNAG 6 cut(s) 191, 432, 597, 912, 1008, 1362
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 3 cut(s) 490, 1076, 1337
HspAI GCGC 1 cut(s) 413
Kzo9I GATC 3 cut(s) 271, 351, 642
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 5 cut(s) 98, 490, 611, 793, 890
Lsp1109I GCAGC 5 cut(s) 189, 787, 1181, 1200, 1267
LweI GCATC 2 cut(s) 346, 866
MaeI CTAG 1 cut(s) 948
MaeIII GTNAC 2 cut(s) 1037, 1185
MalI GATC 3 cut(s) 273, 353, 644
MboI GATC 3 cut(s) 271, 351, 642
MfeI CAATTG 1 cut(s) 861
MhlI GDGCHC 2 cut(s) 197, 240
MluCI AATT 9 cut(s) 145, 397, 511, 710, 861, 970, 1221, 1286, 1337
MlyI GAGTC 6 cut(s) 74, 167, 545, 676, 760, 1108
MmeI TCCRAC 4 cut(s) 154, 793, 971, 1042
MseI TTAA 5 cut(s) 30, 315, 417, 882, 1224
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 4 cut(s) 615, 673, 1109, 1117
MspR9I CCNGG 4 cut(s) 115, 933, 1096, 1118
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 20
MvaI CCWGG 3 cut(s) 115, 933, 1096
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 5 cut(s) 32, 244, 412, 1003, 1277
NciI CCSGG 1 cut(s) 1118
NdeII GATC 3 cut(s) 271, 351, 642
NlaIII CATG 3 cut(s) 490, 1076, 1337
NlaIV GGNNCC 8 cut(s) 94, 237, 591, 613, 820, 868, 892, 1266
NmeAIII GCCGAG 3 cut(s) 260, 654, 919
NmuCI GTSAC 2 cut(s) 1037, 1185
PceI AGGCCT 1 cut(s) 829
PciSI GCTCTTC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 81, 287
PctI GAATGC 1 cut(s) 20
PfeI GAWTC 5 cut(s) 167, 187, 202, 265, 1138
PfoI TCCNGGA 1 cut(s) 1094
PkrI GCNGC 9 cut(s) 64, 179, 249, 478, 802, 1071, 1171, 1215, 1282
PleI GAGTC 6 cut(s) 74, 166, 544, 675, 759, 1107
PpsI GAGTC 6 cut(s) 74, 166, 544, 675, 759, 1107
Psp6I CCWGG 3 cut(s) 113, 931, 1094
PspGI CCWGG 3 cut(s) 113, 931, 1094
PspN4I GGNNCC 8 cut(s) 94, 237, 591, 613, 820, 868, 892, 1266
PspPI GGNCC 6 cut(s) 117, 590, 819, 866, 1106, 1265
RsaI GTAC 2 cut(s) 956, 1160
RsaNI GTAC 2 cut(s) 955, 1159
SapI GCTCTTC 1 cut(s) 157
SaqAI TTAA 5 cut(s) 30, 315, 417, 882, 1224
SatI GCNGC 9 cut(s) 63, 178, 248, 477, 801, 1070, 1170, 1214, 1281
Sau3AI GATC 3 cut(s) 271, 351, 642
Sau96I GGNCC 6 cut(s) 117, 590, 819, 866, 1106, 1265
SchI GAGTC 6 cut(s) 74, 167, 545, 676, 760, 1108
ScrFI CCNGG 4 cut(s) 115, 933, 1096, 1118
SduI GDGCHC 2 cut(s) 197, 240
SfaNI GCATC 2 cut(s) 346, 866
SinI GGWCC 4 cut(s) 117, 819, 866, 1106
Sse9I AATT 9 cut(s) 145, 397, 511, 710, 861, 970, 1221, 1286, 1337
SseBI AGGCCT 1 cut(s) 829
SspMI CTAG 1 cut(s) 948
StuI AGGCCT 1 cut(s) 829
StyD4I CCNGG 4 cut(s) 113, 931, 1094, 1116
StyI CCWWGG 2 cut(s) 777, 1056
TaaI ACNGT 4 cut(s) 772, 811, 1028, 1231
TaqI TCGA 8 cut(s) 69, 337, 354, 401, 560, 639, 705, 761
TasI AATT 9 cut(s) 145, 397, 511, 710, 861, 970, 1221, 1286, 1337
TauI GCSGC 4 cut(s) 65, 250, 479, 1072
TfiI GAWTC 5 cut(s) 167, 187, 202, 265, 1138
Tru1I TTAA 5 cut(s) 30, 315, 417, 882, 1224
Tru9I TTAA 5 cut(s) 30, 315, 417, 882, 1224
TscAI CASTG 1 cut(s) 984
TseFI GTSAC 2 cut(s) 1037, 1185
TseI GCWGC 5 cut(s) 177, 800, 1169, 1213, 1280
Tsp45I GTSAC 2 cut(s) 1037, 1185
TspDTI ATGAA 4 cut(s) 475, 723, 1313, 1350
TspGWI ACGGA 2 cut(s) 88, 903
TspRI CASTG 1 cut(s) 984
VpaK11BI GGWCC 4 cut(s) 117, 819, 866, 1106
XapI RAATTY 3 cut(s) 397, 511, 970
XspI CTAG 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.