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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 201 |
| AclWI | GGATC | 2 cut(s) | 335, 348 |
| AflII | CTTAAG | 1 cut(s) | 11 |
| AgsI | TTSAA | 3 cut(s) | 59, 263, 326 |
| AlwI | GGATC | 2 cut(s) | 335, 348 |
| AoxI | GGCC | 1 cut(s) | 217 |
| AsuHPI | GGTGA | 1 cut(s) | 242 |
| BamHI | GGATCC | 1 cut(s) | 340 |
| BccI | CCATC | 2 cut(s) | 71, 122 |
| BciVI | GTATCC | 1 cut(s) | 292 |
| BfrI | CTTAAG | 1 cut(s) | 11 |
| BfuI | GTATCC | 1 cut(s) | 292 |
| BmiI | GGNNCC | 2 cut(s) | 94, 342 |
| BsaJI | CCNNGG | 1 cut(s) | 285 |
| BseDI | CCNNGG | 1 cut(s) | 285 |
| BshFI | GGCC | 1 cut(s) | 219 |
| BsnI | GGCC | 1 cut(s) | 219 |
| Bsp143I | GATC | 2 cut(s) | 100, 340 |
| Bsp19I | CCATGG | 1 cut(s) | 285 |
| BspANI | GGCC | 1 cut(s) | 219 |
| BspHI | TCATGA | 1 cut(s) | 82 |
| BspLI | GGNNCC | 2 cut(s) | 94, 342 |
| BspPI | GGATC | 2 cut(s) | 335, 348 |
| BspTI | CTTAAG | 1 cut(s) | 11 |
| BssECI | CCNNGG | 1 cut(s) | 285 |
| BssMI | GATC | 2 cut(s) | 100, 340 |
| BssT1I | CCWWGG | 1 cut(s) | 285 |
| Bst4CI | ACNGT | 1 cut(s) | 191 |
| Bst6I | CTCTTC | 1 cut(s) | 147 |
| BstAFI | CTTAAG | 1 cut(s) | 11 |
| BstDSI | CCRYGG | 1 cut(s) | 285 |
| BstKTI | GATC | 2 cut(s) | 103, 343 |
| BstMBI | GATC | 2 cut(s) | 100, 340 |
| BstX2I | RGATCY | 1 cut(s) | 340 |
| BstYI | RGATCY | 1 cut(s) | 340 |
| BsuI | GTATCC | 1 cut(s) | 292 |
| BsuRI | GGCC | 1 cut(s) | 219 |
| BtgI | CCRYGG | 1 cut(s) | 285 |
| CciI | TCATGA | 1 cut(s) | 82 |
| CviAII | CATG | 2 cut(s) | 83, 286 |
| CviJI | RGCY | 2 cut(s) | 95, 219 |
| CviKI_1 | RGCY | 2 cut(s) | 95, 219 |
| DpnI | GATC | 2 cut(s) | 102, 342 |
| DpnII | GATC | 2 cut(s) | 100, 340 |
| Eam1104I | CTCTTC | 1 cut(s) | 147 |
| EarI | CTCTTC | 1 cut(s) | 147 |
| Eco130I | CCWWGG | 1 cut(s) | 285 |
| Eco147I | AGGCCT | 1 cut(s) | 219 |
| Eco32I | GATATC | 1 cut(s) | 27 |
| EcoRV | GATATC | 1 cut(s) | 27 |
| EcoT14I | CCWWGG | 1 cut(s) | 285 |
| ErhI | CCWWGG | 1 cut(s) | 285 |
| FaeI | CATG | 2 cut(s) | 86, 289 |
| FaiI | YATR | 9 cut(s) | 75, 84, 177, 201, 254, 267, 287, 296, 298 |
| FatI | CATG | 2 cut(s) | 82, 285 |
| HaeIII | GGCC | 1 cut(s) | 219 |
| Hin1II | CATG | 2 cut(s) | 86, 289 |
| HinfI | GANTC | 1 cut(s) | 31 |
| HphI | GGTGA | 1 cut(s) | 242 |
| Hpy188III | TCNNGA | 2 cut(s) | 83, 344 |
| HpyAV | CCTTC | 1 cut(s) | 209 |
| HpyCH4III | ACNGT | 1 cut(s) | 191 |
| Hsp92II | CATG | 2 cut(s) | 86, 289 |
| Kzo9I | GATC | 2 cut(s) | 100, 340 |
| LmnI | GCTCC | 1 cut(s) | 92 |
| MaeIII | GTNAC | 1 cut(s) | 191 |
| MalI | GATC | 2 cut(s) | 102, 342 |
| MboI | GATC | 2 cut(s) | 100, 340 |
| MboII | GAAGA | 2 cut(s) | 152, 164 |
| MflI | RGATCY | 1 cut(s) | 340 |
| MluCI | AATT | 3 cut(s) | 39, 59, 270 |
| MlyI | GAGTC | 1 cut(s) | 25 |
| MnlI | CCTC | 2 cut(s) | 15, 271 |
| MseI | TTAA | 2 cut(s) | 12, 38 |
| MslI | CAYNNNNRTG | 1 cut(s) | 177 |
| MspCI | CTTAAG | 1 cut(s) | 11 |
| NcoI | CCATGG | 1 cut(s) | 285 |
| NdeII | GATC | 2 cut(s) | 100, 340 |
| NlaIII | CATG | 2 cut(s) | 86, 289 |
| NlaIV | GGNNCC | 2 cut(s) | 94, 342 |
| PagI | TCATGA | 1 cut(s) | 82 |
| PceI | AGGCCT | 1 cut(s) | 219 |
| PleI | GAGTC | 1 cut(s) | 25 |
| PpsI | GAGTC | 1 cut(s) | 25 |
| PsiI | TTATAA | 1 cut(s) | 201 |
| PspN4I | GGNNCC | 2 cut(s) | 94, 342 |
| PsuI | RGATCY | 1 cut(s) | 340 |
| RseI | CAYNNNNRTG | 1 cut(s) | 177 |
| SaqAI | TTAA | 2 cut(s) | 12, 38 |
| Sau3AI | GATC | 2 cut(s) | 100, 340 |
| SchI | GAGTC | 1 cut(s) | 25 |
| SetI | ASST | 2 cut(s) | 9, 282 |
| SgeI | CNNG | 9 cut(s) | 79, 95, 100, 233, 260, 298, 313, 335, 349 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 177 |
| SmlI | CTYRAG | 1 cut(s) | 11 |
| SmoI | CTYRAG | 1 cut(s) | 11 |
| Sse9I | AATT | 3 cut(s) | 39, 59, 270 |
| SseBI | AGGCCT | 1 cut(s) | 219 |
| StuI | AGGCCT | 1 cut(s) | 219 |
| StyI | CCWWGG | 1 cut(s) | 285 |
| TaaI | ACNGT | 1 cut(s) | 191 |
| TaqI | TCGA | 1 cut(s) | 29 |
| TasI | AATT | 3 cut(s) | 39, 59, 270 |
| Tru1I | TTAA | 2 cut(s) | 12, 38 |
| Tru9I | TTAA | 2 cut(s) | 12, 38 |
| TspDTI | ATGAA | 5 cut(s) | 100, 165, 198, 228, 282 |
| Vha464I | CTTAAG | 1 cut(s) | 11 |