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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 203 |
| AclWI | GGATC | 1 cut(s) | 293 |
| AcuI | CTGAAG | 1 cut(s) | 294 |
| AfaI | GTAC | 2 cut(s) | 227, 310 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 278 |
| AgsI | TTSAA | 2 cut(s) | 191, 297 |
| AjnI | CCWGG | 1 cut(s) | 249 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 277, 309 |
| AlwI | GGATC | 1 cut(s) | 293 |
| AoxI | GGCC | 1 cut(s) | 28 |
| ApeKI | GCWGC | 1 cut(s) | 246 |
| AsuHPI | GGTGA | 3 cut(s) | 178, 229, 280 |
| BaeI | ACNNNNGTAYC | 1 cut(s) | 300 |
| BbvI | GCAGC | 1 cut(s) | 233 |
| BciT130I | CCWGG | 1 cut(s) | 251 |
| BisI | GCNGC | 1 cut(s) | 247 |
| BlsI | GCNGC | 1 cut(s) | 248 |
| Bme1390I | CCNGG | 1 cut(s) | 251 |
| BmrFI | CCNGG | 1 cut(s) | 251 |
| BsaJI | CCNNGG | 1 cut(s) | 213 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 253, 283 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 278 |
| Bse1I | ACTGG | 1 cut(s) | 291 |
| BseBI | CCWGG | 1 cut(s) | 251 |
| BseDI | CCNNGG | 1 cut(s) | 213 |
| BseGI | GGATG | 2 cut(s) | 136, 160 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 278 |
| BseNI | ACTGG | 1 cut(s) | 291 |
| BseRI | GAGGAG | 8 cut(s) | 78, 125, 128, 131, 134, 137, 155, 164 |
| BseXI | GCAGC | 1 cut(s) | 233 |
| BshFI | GGCC | 1 cut(s) | 30 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 278 |
| BsnI | GGCC | 1 cut(s) | 30 |
| Bsp143I | GATC | 2 cut(s) | 285, 318 |
| Bsp19I | CCATGG | 1 cut(s) | 213 |
| BspACI | CCGC | 1 cut(s) | 203 |
| BspANI | GGCC | 1 cut(s) | 30 |
| BspPI | GGATC | 1 cut(s) | 293 |
| BsrI | ACTGG | 1 cut(s) | 291 |
| BssECI | CCNNGG | 1 cut(s) | 213 |
| BssMI | GATC | 2 cut(s) | 285, 318 |
| BssT1I | CCWWGG | 1 cut(s) | 213 |
| Bst2UI | CCWGG | 1 cut(s) | 251 |
| Bst4CI | ACNGT | 1 cut(s) | 50 |
| BstDSI | CCRYGG | 1 cut(s) | 213 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 276 |
| BstF5I | GGATG | 2 cut(s) | 136, 160 |
| BstKTI | GATC | 2 cut(s) | 288, 321 |
| BstMBI | GATC | 2 cut(s) | 285, 318 |
| BstNI | CCWGG | 1 cut(s) | 251 |
| BstSCI | CCNGG | 1 cut(s) | 249 |
| BstV1I | GCAGC | 1 cut(s) | 233 |
| BsuRI | GGCC | 1 cut(s) | 30 |
| BtgI | CCRYGG | 1 cut(s) | 213 |
| BtsCI | GGATG | 2 cut(s) | 136, 160 |
| Csp6I | GTAC | 2 cut(s) | 226, 309 |
| CviAII | CATG | 1 cut(s) | 214 |
| CviJI | RGCY | 1 cut(s) | 30 |
| CviKI_1 | RGCY | 1 cut(s) | 30 |
| CviQI | GTAC | 2 cut(s) | 226, 309 |
| DpnI | GATC | 2 cut(s) | 287, 320 |
| DpnII | GATC | 2 cut(s) | 285, 318 |
| Eco130I | CCWWGG | 1 cut(s) | 213 |
| Eco57I | CTGAAG | 1 cut(s) | 294 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 276 |
| EcoRII | CCWGG | 1 cut(s) | 249 |
| EcoT14I | CCWWGG | 1 cut(s) | 213 |
| ErhI | CCWWGG | 1 cut(s) | 213 |
| FaeI | CATG | 1 cut(s) | 217 |
| FaiI | YATR | 6 cut(s) | 66, 75, 81, 182, 215, 315 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 15, 47 |
| FatI | CATG | 1 cut(s) | 213 |
| Fnu4HI | GCNGC | 1 cut(s) | 247 |
| FokI | GGATG | 2 cut(s) | 143, 167 |
| Fsp4HI | GCNGC | 1 cut(s) | 247 |
| GluI | GCNGC | 1 cut(s) | 247 |
| HaeIII | GGCC | 1 cut(s) | 30 |
| Hin1II | CATG | 1 cut(s) | 217 |
| HphI | GGTGA | 3 cut(s) | 178, 229, 280 |
| Hpy166II | GTNNAC | 1 cut(s) | 136 |
| Hpy188III | TCNNGA | 3 cut(s) | 53, 92, 272 |
| Hpy8I | GTNNAC | 1 cut(s) | 136 |
| Hpy99I | CGWCG | 1 cut(s) | 54 |
| HpyAV | CCTTC | 3 cut(s) | 121, 139, 272 |
| HpyCH4III | ACNGT | 1 cut(s) | 50 |
| Hsp92II | CATG | 1 cut(s) | 217 |
| Kzo9I | GATC | 2 cut(s) | 285, 318 |
| LpnPI | CCDG | 5 cut(s) | 105, 236, 263, 285, 304 |
| Lsp1109I | GCAGC | 1 cut(s) | 233 |
| MalI | GATC | 2 cut(s) | 287, 320 |
| MboI | GATC | 2 cut(s) | 285, 318 |
| MboII | GAAGA | 2 cut(s) | 31, 287 |
| MluCI | AATT | 2 cut(s) | 198, 230 |
| MseI | TTAA | 1 cut(s) | 303 |
| MspR9I | CCNGG | 1 cut(s) | 251 |
| MvaI | CCWGG | 1 cut(s) | 251 |
| NcoI | CCATGG | 1 cut(s) | 213 |
| NdeII | GATC | 2 cut(s) | 285, 318 |
| NlaIII | CATG | 1 cut(s) | 217 |
| PkrI | GCNGC | 1 cut(s) | 248 |
| Psp6I | CCWGG | 1 cut(s) | 249 |
| PspGI | CCWGG | 1 cut(s) | 249 |
| RsaI | GTAC | 2 cut(s) | 227, 310 |
| RsaNI | GTAC | 2 cut(s) | 226, 309 |
| SaqAI | TTAA | 1 cut(s) | 303 |
| SatI | GCNGC | 1 cut(s) | 247 |
| Sau3AI | GATC | 2 cut(s) | 285, 318 |
| ScrFI | CCNGG | 1 cut(s) | 251 |
| SetI | ASST | 3 cut(s) | 168, 283, 310 |
| Sse9I | AATT | 2 cut(s) | 198, 230 |
| SsiI | CCGC | 1 cut(s) | 203 |
| SspI | AATATT | 1 cut(s) | 186 |
| StyD4I | CCNGG | 1 cut(s) | 249 |
| StyI | CCWWGG | 1 cut(s) | 213 |
| TaaI | ACNGT | 1 cut(s) | 50 |
| TasI | AATT | 2 cut(s) | 198, 230 |
| TatI | WGTACW | 1 cut(s) | 225 |
| Tru1I | TTAA | 1 cut(s) | 303 |
| Tru9I | TTAA | 1 cut(s) | 303 |
| TseI | GCWGC | 1 cut(s) | 246 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 276 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 247 |