Rmu_ssc0000252.1_g000036

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000252.1
OG0001042
Physical Location & Seq
Reverse (-)
219152 .. 220576
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000252.1_g000036.1.cds

Sequence Viewer

Length: 1167 bp
atgttttgcaatgttggaaagttttgcaaccagaaaagcggcggcggcagcggctacgtcaacgaggacactgacgacaatgttcatgttattcaatccatcagattaagccatttgttggcaccaccagtacatggcggcgatgatatgaaattacagagagttgcttatagatgtggaccggatactgttgttcccctacatatgagtactggcgtcgtcggctccaaaattattctctttggtggtaaggtttttcctcctgtacgacatcatcgtatttttgaatattcactaagcaaagaagtttgtgtgtttgagacagatccaagcatagatccaaacccggaattgcagccttcccaagtttttaagattagtatgcctgaacctgttctagtagaggtggacgacaaactttatcatttcggtggtcgatgtgactgtgagtcatttgaggtttttgaaccaacacaggatgcctggttgcctctggatgtgcctcctcgaaagtatccctacggcttcactcatggaattgcaagcaccaaaatcctactctggggcagaaatcagaccggagtgtacatgtttgatgcgacccatccggaaaaaggatggatagaattggacccttgtttgggaagctatattccctctattgatgtgctagccttccacatgtcttgtacatgtgattccttaaaacctatggaaccactgcagctactagaatggcctccaaaatttcatagtgcagatcattgtctgttttttcacctcgcagggcaaaaattcggccttattttctcccatgatacattacgtgaggatacattacgtgattactgttatgatcattatgatttcgcttctgattatgatgacgacgataatgaagacaatgaagatgatgataaagacaacgaagacgatggccataataatgataatcacgtcgcctctgacaatgttaattatgatggccttgaggatagtgacaaggtgagagttcttgtcataatctttgaatacgaaattgctgaggaatccatcgacatcaaatacaagttgcttagtactcgctgccttgaatacttatccaagaatcttgaagaagaatcttggactggtatctcacaactgataggtgcctatgtgatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

388

Amino Acids

44.11

Weight (kDa)

4.59

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 121, 1151
AccB7I CCANNNNNTGG 2 cut(s) 118, 134
AccIII TCCGGA 1 cut(s) 607
AciI CCGC 5 cut(s) 39, 42, 45, 51, 138
AclWI GGATC 2 cut(s) 320, 332
AcoI YGGCCR 1 cut(s) 937
AcsI RAATTY 2 cut(s) 746, 794
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 6 cut(s) 132, 211, 267, 587, 691, 1081
AfiI CCNNNNNNNGG 6 cut(s) 118, 134, 562, 614, 640, 641
AflIII ACRYGT 3 cut(s) 588, 681, 692
AgsI TTSAA 6 cut(s) 95, 287, 467, 1031, 1094, 1115
AhdI GACNNNNNGTC 1 cut(s) 448
AjiI CACGTC 1 cut(s) 958
AjnI CCWGG 1 cut(s) 482
AluBI AGCT 2 cut(s) 648, 727
AluI AGCT 2 cut(s) 648, 727
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 2 cut(s) 320, 332
Aor13HI TCCGGA 1 cut(s) 607
AoxI GGCC 4 cut(s) 737, 799, 937, 985
ApeKI GCWGC 4 cut(s) 48, 355, 724, 1086
ApoI RAATTY 2 cut(s) 746, 794
Asp700I GAANNNNTTC 1 cut(s) 393
AspS9I GGNCC 2 cut(s) 179, 631
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 2 cut(s) 770, 1018
AsuNHI GCTAGC 1 cut(s) 670
AvaII GGWCC 2 cut(s) 179, 631
BaeI ACNNNNGTAYC 2 cut(s) 177, 210
BalI TGGCCA 1 cut(s) 939
BanI GGYRCC 2 cut(s) 121, 1151
BbsI GAAGAC 2 cut(s) 906, 936
BbvCI CCTCAGC 1 cut(s) 1044
BbvI GCAGC 4 cut(s) 60, 367, 736, 1073
BccI CCATC 6 cut(s) 107, 612, 612, 929, 977, 1061
BceAI ACGGC 1 cut(s) 538
BciT130I CCWGG 1 cut(s) 484
BciVI GTATCC 3 cut(s) 178, 525, 826
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 3 cut(s) 398, 671, 731
BfmI CTRYAG 1 cut(s) 722
BfuI GTATCC 3 cut(s) 178, 525, 826
BisI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 139, 356, 725, 1087
BlsI GCNGC 9 cut(s) 41, 44, 47, 50, 53, 140, 357, 726, 1088
BmcAI AGTACT 2 cut(s) 211, 1081
Bme1390I CCNGG 2 cut(s) 347, 484
Bme18I GGWCC 2 cut(s) 179, 631
BmeRI GACNNNNNGTC 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 958
BmgT120I GGNCC 2 cut(s) 179, 631
BmiI GGNNCC 5 cut(s) 123, 226, 633, 717, 1153
BmrFI CCNGG 2 cut(s) 347, 484
BmsI GCATC 2 cut(s) 469, 586
BmtI GCTAGC 1 cut(s) 674
BpiI GAAGAC 2 cut(s) 906, 936
Bpu10I CCTNAGC 1 cut(s) 1044
BpuEI CTTGAG 1 cut(s) 1010
BpuMI CCSGG 1 cut(s) 347
BsaAI YACGTR 2 cut(s) 827, 842
BsaHI GRCGYC 1 cut(s) 216
BsaWI WCCGGW 3 cut(s) 181, 578, 607
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 134, 562, 614, 640, 641
Bse1I ACTGG 3 cut(s) 128, 217, 1135
Bse3DI GCAATG 1 cut(s) 16
BseAI TCCGGA 1 cut(s) 607
BseBI CCWGG 1 cut(s) 484
BseGI GGATG 4 cut(s) 484, 502, 604, 623
BseLI CCNNNNNNNGG 6 cut(s) 118, 134, 562, 614, 640, 641
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 1035
BseNI ACTGG 3 cut(s) 128, 217, 1135
BseRI GAGGAG 1 cut(s) 495
BseXI GCAGC 4 cut(s) 60, 367, 736, 1073
BsgI GTGCAG 1 cut(s) 777
BshFI GGCC 4 cut(s) 739, 801, 939, 987
BshNI GGYRCC 2 cut(s) 121, 1151
BsiSI CCGG 4 cut(s) 182, 347, 579, 608
BslI CCNNNNNNNGG 6 cut(s) 118, 134, 562, 614, 640, 641
BsmAI GTCTC 1 cut(s) 314
BsnI GGCC 4 cut(s) 739, 801, 939, 987
Bsp13I TCCGGA 1 cut(s) 607
Bsp1407I TGTACA 2 cut(s) 585, 689
Bsp143I GATC 4 cut(s) 325, 337, 760, 856
BspACI CCGC 5 cut(s) 39, 42, 45, 51, 138
BspANI GGCC 4 cut(s) 739, 801, 939, 987
BspCNI CTCAG 1 cut(s) 1036
BspEI TCCGGA 1 cut(s) 607
BspLI GGNNCC 5 cut(s) 123, 226, 633, 717, 1153
BspMAI CTGCAG 1 cut(s) 726
BspOI GCTAGC 1 cut(s) 674
BspPI GGATC 2 cut(s) 320, 332
BspT107I GGYRCC 2 cut(s) 121, 1151
BsrDI GCAATG 1 cut(s) 16
BsrGI TGTACA 2 cut(s) 585, 689
BsrI ACTGG 3 cut(s) 128, 217, 1135
BssMI GATC 4 cut(s) 325, 337, 760, 856
BssNI GRCGYC 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 3 cut(s) 190, 446, 851
BstACI GRCGYC 1 cut(s) 216
BstAUI TGTACA 2 cut(s) 585, 689
BstBAI YACGTR 2 cut(s) 827, 842
BstC8I GCNNGC 2 cut(s) 544, 672
BstDEI CTNAG 3 cut(s) 296, 1044, 1076
BstF5I GGATG 4 cut(s) 484, 502, 604, 623
BstKTI GATC 4 cut(s) 328, 340, 763, 859
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 4 cut(s) 325, 337, 760, 856
BstMWI GCNNNNNNNGC 4 cut(s) 45, 48, 51, 222
BstNI CCWGG 1 cut(s) 484
BstNSI RCATGY 3 cut(s) 592, 685, 696
BstSCI CCNGG 2 cut(s) 345, 482
BstSFI CTRYAG 1 cut(s) 722
BstV1I GCAGC 4 cut(s) 60, 367, 736, 1073
BstV2I GAAGAC 2 cut(s) 906, 936
BstX2I RGATCY 2 cut(s) 325, 337
BstYI RGATCY 2 cut(s) 325, 337
BsuI GTATCC 3 cut(s) 178, 525, 826
BsuRI GGCC 4 cut(s) 739, 801, 939, 987
BtgZI GCGATG 1 cut(s) 156
BtrI CACGTC 1 cut(s) 958
BtsCI GGATG 4 cut(s) 484, 502, 604, 623
BtsI GCAGTG 1 cut(s) 719
BtsIMutI CAGTG 2 cut(s) 69, 719
Cac8I GCNNGC 2 cut(s) 544, 672
Cfr13I GGNCC 2 cut(s) 179, 631
CseI GACGC 1 cut(s) 205
Csp6I GTAC 6 cut(s) 131, 210, 266, 586, 690, 1080
CspCI CAANNNNNGTGG 2 cut(s) 668, 703
CviAII CATG 7 cut(s) 86, 134, 533, 589, 682, 693, 815
CviQI GTAC 6 cut(s) 131, 210, 266, 586, 690, 1080
DdeI CTNAG 3 cut(s) 296, 1044, 1076
DpnI GATC 4 cut(s) 327, 339, 762, 858
DpnII GATC 4 cut(s) 325, 337, 760, 856
DriI GACNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 937
Eam1105I GACNNNNNGTC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 179, 631
EcoRII CCWGG 1 cut(s) 482
FaeI CATG 7 cut(s) 89, 137, 536, 592, 685, 696, 818
FatI CATG 7 cut(s) 85, 133, 532, 588, 681, 692, 814
FauNDI CATATG 1 cut(s) 204
FbaI TGATCA 1 cut(s) 856
Fnu4HI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 139, 356, 725, 1087
FokI GGATG 4 cut(s) 491, 509, 591, 630
Fsp4HI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 139, 356, 725, 1087
FspBI CTAG 3 cut(s) 398, 671, 731
GluI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 139, 356, 725, 1087
HaeIII GGCC 4 cut(s) 739, 801, 939, 987
HapII CCGG 4 cut(s) 182, 347, 579, 608
HgaI GACGC 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 7 cut(s) 89, 137, 536, 592, 685, 696, 818
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 5 cut(s) 449, 698, 1049, 1108, 1121
HpaII CCGG 4 cut(s) 182, 347, 579, 608
HphI GGTGA 2 cut(s) 770, 1018
Hpy166II GTNNAC 4 cut(s) 61, 179, 409, 586
Hpy188I TCNGA 4 cut(s) 104, 576, 877, 967
Hpy188III TCNNGA 3 cut(s) 494, 608, 1112
Hpy8I GTNNAC 4 cut(s) 61, 179, 409, 586
Hpy99I CGWCG 4 cut(s) 221, 224, 893, 962
HpyAV CCTTC 2 cut(s) 369, 685
HpyCH4III ACNGT 3 cut(s) 190, 446, 851
HpyCH4IV ACGT 4 cut(s) 57, 826, 841, 957
HpyCH4V TGCA 6 cut(s) 9, 27, 355, 542, 724, 758
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 48, 51, 222
HpyF3I CTNAG 3 cut(s) 296, 1044, 1076
HpySE526I ACGT 4 cut(s) 57, 826, 841, 957
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 7 cut(s) 89, 137, 536, 592, 685, 696, 818
Kpn2I TCCGGA 1 cut(s) 607
Ksp22I TGATCA 1 cut(s) 856
Kzo9I GATC 4 cut(s) 325, 337, 760, 856
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 4 cut(s) 60, 367, 736, 1073
LweI GCATC 2 cut(s) 469, 586
MaeI CTAG 3 cut(s) 398, 671, 731
MaeII ACGT 4 cut(s) 57, 826, 841, 957
MaeIII GTNAC 2 cut(s) 440, 998
MalI GATC 4 cut(s) 327, 339, 762, 858
MboI GATC 4 cut(s) 325, 337, 760, 856
MboII GAAGA 5 cut(s) 911, 920, 941, 1127, 1130
MflI RGATCY 2 cut(s) 325, 337
MlsI TGGCCA 1 cut(s) 939
MluCI AATT 9 cut(s) 152, 231, 350, 537, 626, 746, 794, 976, 1038
MluNI TGGCCA 1 cut(s) 939
MlyI GAGTC 1 cut(s) 458
Mox20I TGGCCA 1 cut(s) 939
MroI TCCGGA 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 939
MseI TTAA 4 cut(s) 107, 372, 704, 975
MslI CAYNNNNRTG 2 cut(s) 429, 945
Msp20I TGGCCA 1 cut(s) 939
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 4 cut(s) 182, 347, 579, 608
MspR9I CCNGG 2 cut(s) 347, 484
MvaI CCWGG 1 cut(s) 484
MwoI GCNNNNNNNGC 4 cut(s) 45, 48, 51, 222
NciI CCSGG 1 cut(s) 347
NdeI CATATG 1 cut(s) 204
NdeII GATC 4 cut(s) 325, 337, 760, 856
NheI GCTAGC 1 cut(s) 670
NlaIII CATG 7 cut(s) 89, 137, 536, 592, 685, 696, 818
NlaIV GGNNCC 5 cut(s) 123, 226, 633, 717, 1153
NmuCI GTSAC 2 cut(s) 440, 998
NspI RCATGY 3 cut(s) 592, 685, 696
PciI ACATGT 3 cut(s) 588, 681, 692
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 4 cut(s) 698, 1049, 1108, 1121
PflMI CCANNNNNTGG 2 cut(s) 118, 134
PkrI GCNGC 9 cut(s) 41, 44, 47, 50, 53, 140, 357, 726, 1088
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
Ppu21I YACGTR 2 cut(s) 827, 842
PscI ACATGT 3 cut(s) 588, 681, 692
Psp6I CCWGG 1 cut(s) 482
PspGI CCWGG 1 cut(s) 482
PspN4I GGNNCC 5 cut(s) 123, 226, 633, 717, 1153
PspPI GGNCC 2 cut(s) 179, 631
PstI CTGCAG 1 cut(s) 726
PsuI RGATCY 2 cut(s) 325, 337
RsaI GTAC 6 cut(s) 132, 211, 267, 587, 691, 1081
RsaNI GTAC 6 cut(s) 131, 210, 266, 586, 690, 1080
RseI CAYNNNNRTG 2 cut(s) 429, 945
SaqAI TTAA 4 cut(s) 107, 372, 704, 975
SatI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 139, 356, 725, 1087
Sau3AI GATC 4 cut(s) 325, 337, 760, 856
Sau96I GGNCC 2 cut(s) 179, 631
ScaI AGTACT 2 cut(s) 211, 1081
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 347, 484
SfaNI GCATC 2 cut(s) 469, 586
SfcI CTRYAG 1 cut(s) 722
SinI GGWCC 2 cut(s) 179, 631
SmiMI CAYNNNNRTG 2 cut(s) 429, 945
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
Sse9I AATT 9 cut(s) 152, 231, 350, 537, 626, 746, 794, 976, 1038
SsiI CCGC 5 cut(s) 39, 42, 45, 51, 138
SspI AATATT 1 cut(s) 290
SspMI CTAG 3 cut(s) 398, 671, 731
StyD4I CCNGG 2 cut(s) 345, 482
TaaI ACNGT 3 cut(s) 190, 446, 851
TaiI ACGT 4 cut(s) 60, 829, 844, 960
TaqI TCGA 3 cut(s) 436, 508, 1056
TasI AATT 9 cut(s) 152, 231, 350, 537, 626, 746, 794, 976, 1038
TatI WGTACW 5 cut(s) 130, 209, 585, 689, 1079
TauI GCSGC 5 cut(s) 42, 45, 48, 54, 141
TfiI GAWTC 4 cut(s) 698, 1049, 1108, 1121
Tru1I TTAA 4 cut(s) 107, 372, 704, 975
Tru9I TTAA 4 cut(s) 107, 372, 704, 975
TscAI CASTG 2 cut(s) 76, 726
TseFI GTSAC 2 cut(s) 440, 998
TseI GCWGC 4 cut(s) 48, 355, 724, 1086
Tsp45I GTSAC 2 cut(s) 440, 998
TspDTI ATGAA 5 cut(s) 74, 164, 740, 912, 921
TspRI CASTG 2 cut(s) 76, 726
Van91I CCANNNNNTGG 2 cut(s) 118, 134
VpaK11BI GGWCC 2 cut(s) 179, 631
XapI RAATTY 2 cut(s) 746, 794
XceI RCATGY 3 cut(s) 592, 685, 696
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 3 cut(s) 398, 671, 731
ZrmI AGTACT 2 cut(s) 211, 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.