Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0000550

Physical Location & Seq

Genomic Coordinates Forward (+)

14713732 .. 14719690

Gene Structure 5959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1046200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGATTGTAACCAGAGTTGTCCCTCAACCCTATATAAAGACACCCCCTCACCCCTCTTTTGCCTCACCATTTGCTTCTCACCAATTACTCATTACCCTCAACTTTGATGCTCTCTCTCACTCCCTCATTATTTTTCATTTTCCATTAGTCTCCTCTTCATTTTTTTTCCTCCACCTCTTAATTTCTGGGAAGAAGATGGCGTCGAATGGGAACGGAGAGTTCAGCTTTGCGAGCGGAAACGGAACGGTGGCAACGGCGGCAAACGGTAACGGAACGGCGAGGAACGGGCTGGCGAAGATCCAGACGCAGAAGAAGGACAATGGAGTGTGTCACGACGACAGTACGCCACCTGTGAAGGCGCAGACGATCGACGAGCTCCACTCGCTGCAGAAGAAGAAGTCGGCACCCACCACCCCAATCAAGGGCACTCAGGGTGGTGCCTTTGCCGTCACCCTCTCTGAGGAGGAGCGACAAAAGCAGCAGCTCCAGTCCATCAGTGCATCTTTGGCATCGCTGACGAGAGAGACGGGACCAAAGGTTGTTCGAGGAGACCCGGCCAAGCAGTCCGATACCCCCAAGGTTTCGTCGCACGTGTCGCACCACCACCACTTCACGCCCACCATTAGCGTCAGTGACAGCGCCTTGAAGTTCACACATGTCCTCTACAACCTTTCTCCAGCTGAGTTATATGAGCAGGCAATAAAGTATGAGAAAGGGTCGTTCGTTACGTCAACCGGCGCGCTAGCAACTCTTTCTGGAGCCAAGACTGGTCGCTCTCCAAGAGACAAACGCGTCGTCAAGGATGACACCACCCGGGACGACCTTTGGTGGGGAAGCGGCTCACCTAACATTGAAATGGACGAGCATACGTTCATGGTCAACAGAGAAAGAGCTGTGGATTACTTGAATTCTTTGGACAAGGTCTTTGTAAATGATCAATTTTTGAATTGGGATCCAGAAAACAGAATCAAAGTTCGGGTCGTGTCTGCCAGGGCTTATCACTCATTGTTTATGCACAACATGTGTATCCGACCCACTCCTGAAGAGCTGGAGAATTTTGGTACTCCGGACTTCACTATATACAATGCCGGCCAGTTCCCATGTAATCGTTACACCCACTACATGACATCCTCTACTAGCATAGACCTTAATCTTGCTAGGAGGGAAATGGTCATCCTCGGCACCATGTACGCTGGGGAAATGAAGAAAGGTCTTTTCAGTGTCATGCATTATCTCATGCCTAAGCGACAAATCCTATCCCTGCATTCTGGCTGCAATATGGGGAAAGATGGAGATGTTGCCCTTTTCTTTGGATTGTCAGGTACTGGAAAGACGACTCTGTCTACGGATCACAATAGGTACTTGATTGGAGATGATGAACACTGCTGGACTGAGAACGGTGTGTCAAATATCGAAGGTGGTTGCTACGCCAAGTGCATTGACCTGTCAAGGGAGAAAGAACCTGATATTTGGAACGCCATCAAGTTTGGCGCTGTGTTGGAAAACATCGTGTTTGATGAGTATACACGAGAGGTGGACTATTCTGACAAATCTGTTACAGAGAACACTCGTGCAGCTTATCCCATCGAATACATTCCCAATGCAAAGATACCATGCGTTGGTCCACATCCAAAGAATGTCATACTTTTGGCATGCGATGCATTTGGTGTGCTCCCTCCTGTGAGCAAGTTAAACCTTGCTCAGACCATGTACCACTTCATCAGTGGCTACACTGCTCTGGTTGCTGGAACTGAAGATGGTATCAAGGAGCCAACAGCAACATTCTCAGCATGCTTCGGTGCAGCATTTATAATGTTGCATCCTACTAAGTATGCAGCAATGCTGGCTGAGAAGATGCAGAAGCACGGTGCAACAGGATGGCTGGTTAACACTGGCTGGTCCGGTGGAAGCTATGGATCCGGAAAGCGCATTAAGTTGCCCTACACCAGGAAAATCATTGACGCCATCCACTCCGGCAGCCTCTTAAATGCGAAGTACAAGAAGACTGAAGTGTTTGGACTTGAAATCCCCACTGAGGTCGAGGGAGTGCCTTCAGAAATACTGGATCCAATGAACACTTGGTCGGACAAGAATGCATACAATGAAACTCTGATGAAGCTAGCTGGCCTCTTCAAGAGGAACTTTGAGACTTTCACAAACTACAAGATTGGGAAGGACAACAAGCTGACCGAGGAGATACTTGCGGCCGGACCAAATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004611 GO:0004612 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006094 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0008150 GO:0008152 GO:0009058 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0016020 GO:0016036 GO:0016051 GO:0016829 GO:0016830 GO:0016831 GO:0019318 GO:0019319 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0033554 GO:0042221 GO:0042594 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046364 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070013 GO:0071496 GO:0071704 GO:0098542 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PEPCK_ATP

Protein Analysis

741

Amino Acids

81.75

Weight (kDa)

7.65

Isoelectric Point (pI)

34.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPCK_ATP	PF01293	219 - 686	6.7e-215	Phosphoenolpyruvate carboxykinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37870 AT5G65690 AT5G65690 AT5G65690 AT5G65690
fragaria_vesca	FvH4_1g18831 FvH4_1g18850 FvH4_1g18850 FvH4_1g24570 FvH4_4g10540 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_7g04810
malus_domestica	MD01G1046200.v1.1 MD08G1207000.v1.1 MD15G1391200.v1.1 MD15G1405400.v1.1
prunus_persica	Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.6G210900_v2.0.a1 Prupe.6G211000_v2.0.a1
pyrus_communis	pycom01g07230 pycom08g17910 pycom15g26890 pycom16g22360
rosa_chinensis	RchiOBHm_Chr2g0109711 RchiOBHm_Chr2g0109721 RchiOBHm_Chr5g0054131 RchiOBHm_Chr5g0054141 RchiOBHm_Chr5g0054151 RchiOBHm_Chr7g0233021
rosa_laevigata	RLG00000001305 RLG00000017759 RLG00000017760 RLG00000030254 RLG00000030261 RLG00000030264
rosa_multiflora	Rmu_sc0000507.1_g000005 Rmu_sc0000509.1_g000040 Rmu_sc0002076.1_g000010 Rmu_sc0006889.1_g000016 Rmu_sc0006889.1_g000018 Rmu_ssc0000018.1_g000034
rosa_roxburghii	Rroxscaffold_2G00134000 Rroxscaffold_3G00227740 Rroxscaffold_4G00326310 Rroxscaffold_4G00326340
rosa_rugosa	Rorug02G0162100 Rorug02G0162200 Rorug02G0162300 Rorug07G0277900.1
rosa_samantha	Rh2AG214600 Rh2AG214700 Rh2BG224900 Rh2BG225000 Rh2BG225100 Rh2CG216800 Rh2CG216900 Rh2DG220100 Rh2DG220200 Rh5AG354400 Rh5AG354500 Rh5DG381200 Rh5DG381300 Rh7BG405700 Rh7CG452300 Rh7DG421600
rosa_wichuraiana	Rw0G009650 Rw0G009660 Rw1G004940 Rw1G004970 Rw2G016580 Rw5G033400 Rw7G035700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1811
AasI	GACNNNNNNGTC	2 cut(s)	791, 1339
AccB1I	GGYRCC	3 cut(s)	403, 437, 1181
AccB7I	CCANNNNNTGG	1 cut(s)	1619
AccBSI	CCGCTC	1 cut(s)	234
AccI	GTMKAC	2 cut(s)	1343, 1523
AccII	CGCG	2 cut(s)	740, 792
AccIII	TCCGGA	2 cut(s)	1066, 1919
AciI	CCGC	4 cut(s)	234, 257, 837, 2204
AclWI	GGATC	8 cut(s)	292, 947, 960, 1356, 1911, 1924, 2060, 2073
AcoI	YGGCCR	3 cut(s)	555, 1090, 2205
AcsI	RAATTY	3 cut(s)	907, 1054, 2215
AcuI	CTGAAG	4 cut(s)	1062, 1773, 2028, 2037
AcvI	CACGTG	1 cut(s)	592
AcyI	GRCGYC	2 cut(s)	200, 1962
AfaI	GTAC	7 cut(s)	343, 1063, 1190, 1324, 1361, 1712, 1997
AfiI	CCNNNNNNNGG	8 cut(s)	421, 422, 460, 829, 1450, 1619, 1947, 2035
AflIII	ACRYGT	4 cut(s)	591, 655, 790, 1020
AgsI	TTSAA	6 cut(s)	646, 854, 907, 946, 2024, 2134
AjnI	CCWGG	2 cut(s)	989, 1946
Alw21I	GWGCWC	2 cut(s)	378, 1674
Alw26I	GTCTC	5 cut(s)	154, 518, 543, 777, 2141
AlwI	GGATC	8 cut(s)	292, 947, 960, 1356, 1911, 1924, 2060, 2073
AlwNI	CAGNNNCTG	1 cut(s)	1325
Ama87I	CYCGRG	1 cut(s)	813
Aor13HI	TCCGGA	2 cut(s)	1066, 1919
AoxI	GGCC	4 cut(s)	555, 1090, 2125, 2205
ApeKI	GCWGC	8 cut(s)	385, 478, 481, 1272, 1574, 1802, 1835, 1977
ApoI	RAATTY	3 cut(s)	907, 1054, 2215
ArsI	GACNNNNNNTTYG	6 cut(s)	780, 812, 908, 940, 963, 995
Asp700I	GAANNNNTTC	1 cut(s)	1593
AspLEI	GCGC	6 cut(s)	361, 641, 740, 742, 1493, 1929
AspS9I	GGNCC	4 cut(s)	530, 1622, 1899, 2210
AsuC2I	CCSGG	3 cut(s)	554, 814, 815
AsuHPI	GGTGA	5 cut(s)	41, 57, 71, 442, 834
AsuNHI	GCTAGC	2 cut(s)	742, 2119
AvaI	CYCGRG	1 cut(s)	813
AvaII	GGWCC	4 cut(s)	530, 1622, 1899, 2210
BaeGI	GKGCMC	1 cut(s)	428
BaeI	ACNNNNGTAYC	2 cut(s)	1009, 1042
BamHI	GGATCC	3 cut(s)	952, 1916, 2065
BanI	GGYRCC	3 cut(s)	403, 437, 1181
BanII	GRGCYC	1 cut(s)	378
BauI	CACGAG	2 cut(s)	1527, 1569
BbrPI	CACGTG	1 cut(s)	592
BbsI	GAAGAC	1 cut(s)	2009
Bbv12I	GWGCWC	2 cut(s)	378, 1674
BbvI	GCAGC	8 cut(s)	372, 490, 493, 1259, 1586, 1814, 1847, 1989
BccI	CCATC	8 cut(s)	190, 500, 1283, 1487, 1592, 1751, 1872, 1973
BceAI	ACGGC	3 cut(s)	270, 291, 431
BciT130I	CCWGG	2 cut(s)	991, 1948
BciVI	GTATCC	1 cut(s)	1037
BclI	TGATCA	1 cut(s)	934
BcnI	CCSGG	3 cut(s)	554, 814, 815
BcoDI	GTCTC	5 cut(s)	154, 518, 543, 777, 2141
BfaI	CTAG	4 cut(s)	743, 1137, 1158, 2120
BfmI	CTRYAG	1 cut(s)	386
BfoI	RGCGCY	2 cut(s)	642, 1494
BfuI	GTATCC	1 cut(s)	1037
Bme1390I	CCNGG	5 cut(s)	554, 814, 815, 991, 1948
Bme18I	GGWCC	4 cut(s)	530, 1622, 1899, 2210
BmeT110I	CYCGRG	1 cut(s)	813
BmgT120I	GGNCC	4 cut(s)	530, 1622, 1899, 2210
BmiI	GGNNCC	9 cut(s)	405, 439, 531, 760, 954, 1183, 1770, 1918, 2067
BmrFI	CCNGG	5 cut(s)	554, 814, 815, 991, 1948
BmsI	GCATC	6 cut(s)	97, 509, 518, 1648, 1828, 1845
BmtI	GCTAGC	2 cut(s)	746, 2123
BpiI	GAAGAC	1 cut(s)	2009
BplI	GAGNNNNNCTC	2 cut(s)	365, 397
BpmI	CTGGAG	4 cut(s)	470, 660, 777, 1070
Bpu10I	CCTNAGC	1 cut(s)	1242
BpuMI	CCSGG	3 cut(s)	554, 814, 815
BsaAI	YACGTR	1 cut(s)	592
BsaHI	GRCGYC	2 cut(s)	200, 1962
BsaI	GGTCTC	1 cut(s)	543
BsaJI	CCNNGG	5 cut(s)	576, 813, 990, 1177, 2190
BsaWI	WCCGGW	3 cut(s)	1066, 1901, 1919
Bsc4I	CCNNNNNNNGG	8 cut(s)	421, 422, 460, 829, 1450, 1619, 1947, 2035
Bse118I	RCCGGY	2 cut(s)	734, 1088
Bse1I	ACTGG	6 cut(s)	487, 772, 1093, 1330, 1897, 2067
Bse3DI	GCAATG	1 cut(s)	1845
BseAI	TCCGGA	2 cut(s)	1066, 1919
BseBI	CCWGG	2 cut(s)	991, 1948
BseDI	CCNNGG	5 cut(s)	576, 813, 990, 1177, 2190
BseGI	GGATG	7 cut(s)	808, 1127, 1173, 1627, 1819, 1883, 1965
BseLI	CCNNNNNNNGG	8 cut(s)	421, 422, 460, 829, 1450, 1619, 1947, 2035
BseMI	GCAATG	1 cut(s)	1845
BseMII	CTCAG	8 cut(s)	443, 450, 672, 1383, 1715, 1800, 1839, 2025
BseNI	ACTGG	6 cut(s)	487, 772, 1093, 1330, 1897, 2067
BsePI	GCGCGC	1 cut(s)	738
BseRI	GAGGAG	5 cut(s)	142, 476, 479, 561, 2207
BseSI	GKGCMC	1 cut(s)	428
BseX3I	CGGCCG	1 cut(s)	2205
BseXI	GCAGC	8 cut(s)	372, 490, 493, 1259, 1586, 1814, 1847, 1989
BseYI	CCCAGC	1 cut(s)	1193
BsgI	GTGCAG	2 cut(s)	1593, 1821
Bsh1236I	CGCG	2 cut(s)	740, 792
Bsh1285I	CGRYCG	2 cut(s)	369, 2208
BshFI	GGCC	4 cut(s)	557, 1092, 2127, 2207
BshNI	GGYRCC	3 cut(s)	403, 437, 1181
BsiEI	CGRYCG	2 cut(s)	369, 2208
BsiHKAI	GWGCWC	2 cut(s)	378, 1674
BsiHKCI	CYCGRG	1 cut(s)	813
BsiSI	CCGG	9 cut(s)	554, 735, 814, 1067, 1089, 1902, 1920, 1974, 2208
BslFI	GGGAC	3 cut(s)	5, 543, 830
BslI	CCNNNNNNNGG	8 cut(s)	421, 422, 460, 829, 1450, 1619, 1947, 2035
BsmAI	GTCTC	5 cut(s)	154, 518, 543, 777, 2141
BsmBI	CGTCTC	1 cut(s)	518
BsmFI	GGGAC	3 cut(s)	5, 543, 830
BsmI	GAATGC	2 cut(s)	1264, 2098
BsnI	GGCC	4 cut(s)	557, 1092, 2127, 2207
Bso31I	GGTCTC	1 cut(s)	543
BsoBI	CYCGRG	1 cut(s)	813
Bsp1286I	GDGCHC	3 cut(s)	378, 428, 1674
Bsp13I	TCCGGA	2 cut(s)	1066, 1919
Bsp143I	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
BspACI	CCGC	4 cut(s)	234, 257, 837, 2204
BspANI	GGCC	4 cut(s)	557, 1092, 2127, 2207
BspCNI	CTCAG	8 cut(s)	442, 451, 673, 1384, 1714, 1799, 1840, 2026
BspEI	TCCGGA	2 cut(s)	1066, 1919
BspFNI	CGCG	2 cut(s)	740, 792
BspLI	GGNNCC	9 cut(s)	405, 439, 531, 760, 954, 1183, 1770, 1918, 2067
BspMAI	CTGCAG	1 cut(s)	390
BspOI	GCTAGC	2 cut(s)	746, 2123
BspPI	GGATC	8 cut(s)	292, 947, 960, 1356, 1911, 1924, 2060, 2073
BspQI	GCTCTTC	1 cut(s)	1038
BspT107I	GGYRCC	3 cut(s)	403, 437, 1181
BspTNI	GGTCTC	1 cut(s)	543
BsrBI	CCGCTC	1 cut(s)	234
BsrDI	GCAATG	1 cut(s)	1845
BsrFI	RCCGGY	2 cut(s)	734, 1088
BsrI	ACTGG	6 cut(s)	487, 772, 1093, 1330, 1897, 2067
BssAI	RCCGGY	2 cut(s)	734, 1088
BssECI	CCNNGG	5 cut(s)	576, 813, 990, 1177, 2190
BssHII	GCGCGC	1 cut(s)	738
BssMI	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
BssNAI	GTATAC	1 cut(s)	1524
BssNI	GRCGYC	2 cut(s)	200, 1962
BssSI	CACGAG	2 cut(s)	1527, 1569
BssT1I	CCWWGG	1 cut(s)	576
Bst1107I	GTATAC	1 cut(s)	1524
Bst2BI	CACGAG	2 cut(s)	1527, 1569
Bst2UI	CCWGG	2 cut(s)	991, 1948
Bst4CI	ACNGT	5 cut(s)	247, 266, 341, 1400, 1868
Bst6I	CTCTTC	3 cut(s)	160, 1038, 2135
BstACI	GRCGYC	2 cut(s)	200, 1962
BstAPI	GCANNNNNTGC	1 cut(s)	1658
BstBAI	YACGTR	1 cut(s)	592
BstENI	CCTNNNNNAGG	2 cut(s)	458, 1945
BstF5I	GGATG	7 cut(s)	808, 1127, 1173, 1627, 1819, 1883, 1965
BstFNI	CGCG	2 cut(s)	740, 792
BstH2I	RGCGCY	2 cut(s)	642, 1494
BstHHI	GCGC	6 cut(s)	361, 641, 740, 742, 1493, 1929
BstKTI	GATC	7 cut(s)	300, 369, 937, 955, 1351, 1919, 2068
BstMAI	GTCTC	5 cut(s)	154, 518, 543, 777, 2141
BstMBI	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
BstMCI	CGRYCG	2 cut(s)	369, 2208
BstMWI	GCNNNNNNNGC	9 cut(s)	231, 257, 382, 475, 506, 595, 1658, 1742, 1844
BstNI	CCWGG	2 cut(s)	991, 1948
BstNSI	RCATGY	4 cut(s)	659, 1024, 1656, 1794
BstSCI	CCNGG	5 cut(s)	552, 812, 813, 989, 1946
BstSFI	CTRYAG	1 cut(s)	386
BstSLI	GKGCMC	1 cut(s)	428
BstUI	CGCG	2 cut(s)	740, 792
BstV1I	GCAGC	8 cut(s)	372, 490, 493, 1259, 1586, 1814, 1847, 1989
BstV2I	GAAGAC	1 cut(s)	2009
BstX2I	RGATCY	4 cut(s)	297, 952, 1916, 2065
BstYI	RGATCY	4 cut(s)	297, 952, 1916, 2065
BstZ17I	GTATAC	1 cut(s)	1524
BstZI	CGGCCG	1 cut(s)	2205
BsuI	GTATCC	1 cut(s)	1037
BsuRI	GGCC	4 cut(s)	557, 1092, 2127, 2207
BtgZI	GCGATG	2 cut(s)	495, 1671
BtsCI	GGATG	7 cut(s)	808, 1127, 1173, 1627, 1819, 1883, 1965
BtsI	GCAGTG	2 cut(s)	1381, 1731
BtsIMutI	CAGTG	8 cut(s)	502, 637, 1225, 1381, 1729, 1731, 1890, 2031
CaiI	CAGNNNCTG	1 cut(s)	1325
CfoI	GCGC	6 cut(s)	361, 641, 740, 742, 1493, 1929
Cfr10I	RCCGGY	2 cut(s)	734, 1088
Cfr13I	GGNCC	4 cut(s)	530, 1622, 1899, 2210
Cfr9I	CCCGGG	1 cut(s)	813
CseI	GACGC	5 cut(s)	189, 313, 616, 781, 1970
Csp6I	GTAC	7 cut(s)	342, 1062, 1189, 1323, 1360, 1711, 1996
CviQI	GTAC	7 cut(s)	342, 1062, 1189, 1323, 1360, 1711, 1996
DpnI	GATC	7 cut(s)	299, 368, 936, 954, 1350, 1918, 2067
DpnII	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
DrdI	GACNNNNNNGTC	2 cut(s)	791, 1339
DseDI	GACNNNNNNGTC	2 cut(s)	791, 1339
EaeI	YGGCCR	3 cut(s)	555, 1090, 2205
EagI	CGGCCG	1 cut(s)	2205
Eam1104I	CTCTTC	3 cut(s)	160, 1038, 2135
EarI	CTCTTC	3 cut(s)	160, 1038, 2135
Ecl136II	GAGCTC	1 cut(s)	376
EclXI	CGGCCG	1 cut(s)	2205
Eco130I	CCWWGG	1 cut(s)	576
Eco24I	GRGCYC	1 cut(s)	378
Eco31I	GGTCTC	1 cut(s)	543
Eco47I	GGWCC	4 cut(s)	530, 1622, 1899, 2210
Eco52I	CGGCCG	1 cut(s)	2205
Eco53kI	GAGCTC	1 cut(s)	376
Eco57I	CTGAAG	4 cut(s)	1062, 1773, 2028, 2037
Eco72I	CACGTG	1 cut(s)	592
Eco88I	CYCGRG	1 cut(s)	813
EcoICRI	GAGCTC	1 cut(s)	376
EcoNI	CCTNNNNNAGG	2 cut(s)	458, 1945
EcoRI	GAATTC	1 cut(s)	907
EcoRII	CCWGG	2 cut(s)	989, 1946
EcoT14I	CCWWGG	1 cut(s)	576
EcoT22I	ATGCAT	3 cut(s)	1230, 1663, 2098
EcoT38I	GRGCYC	1 cut(s)	378
ErhI	CCWWGG	1 cut(s)	576
Esp3I	CGTCTC	1 cut(s)	518
FalI	AAGNNNNNCTT	2 cut(s)	2126, 2158
FaqI	GGGAC	3 cut(s)	5, 543, 830
FbaI	TGATCA	1 cut(s)	934
FblI	GTMKAC	2 cut(s)	1343, 1523
FokI	GGATG	7 cut(s)	815, 1114, 1160, 1614, 1806, 1890, 1952
FriOI	GRGCYC	1 cut(s)	378
FspBI	CTAG	4 cut(s)	743, 1137, 1158, 2120
GlaI	GCGC	6 cut(s)	360, 640, 739, 741, 1492, 1928
GsaI	CCCAGC	1 cut(s)	1197
GsuI	CTGGAG	4 cut(s)	470, 660, 777, 1070
HaeII	RGCGCY	2 cut(s)	642, 1494
HaeIII	GGCC	4 cut(s)	557, 1092, 2127, 2207
HapII	CCGG	9 cut(s)	554, 735, 814, 1067, 1089, 1902, 1920, 1974, 2208
HgaI	GACGC	5 cut(s)	189, 313, 616, 781, 1970
HhaI	GCGC	6 cut(s)	361, 641, 740, 742, 1493, 1929
Hin1I	GRCGYC	2 cut(s)	200, 1962
Hin6I	GCGC	6 cut(s)	359, 639, 738, 740, 1491, 1927
HinP1I	GCGC	6 cut(s)	359, 639, 738, 740, 1491, 1927
HincII	GTYRAC	3 cut(s)	732, 880, 1888
HindII	GTYRAC	3 cut(s)	732, 880, 1888
HinfI	GANTC	2 cut(s)	966, 1336
HpaI	GTTAAC	1 cut(s)	1888
HpaII	CCGG	9 cut(s)	554, 735, 814, 1067, 1089, 1902, 1920, 1974, 2208
HphI	GGTGA	5 cut(s)	41, 57, 71, 442, 834
Hpy166II	GTNNAC	8 cut(s)	651, 732, 880, 1344, 1524, 1537, 1625, 1888
Hpy188I	TCNGA	8 cut(s)	460, 568, 1031, 1546, 1704, 2056, 2086, 2112
Hpy188III	TCNNGA	8 cut(s)	301, 332, 756, 956, 1040, 1067, 1920, 2134
Hpy8I	GTNNAC	8 cut(s)	651, 732, 880, 1344, 1524, 1537, 1625, 1888
Hpy99I	CGWCG	5 cut(s)	205, 338, 374, 589, 797
HpyAV	CCTTC	5 cut(s)	307, 349, 1409, 2061, 2167
HpyCH4III	ACNGT	5 cut(s)	247, 266, 341, 1400, 1868
HpyCH4IV	ACGT	3 cut(s)	591, 728, 869
HpyF10VI	GCNNNNNNNGC	9 cut(s)	231, 257, 382, 475, 506, 595, 1658, 1742, 1844
HpySE526I	ACGT	3 cut(s)	591, 728, 869
Hsp92I	GRCGYC	2 cut(s)	200, 1962
HspAI	GCGC	6 cut(s)	359, 639, 738, 740, 1491, 1927
Kpn2I	TCCGGA	2 cut(s)	1066, 1919
KroI	GCCGGC	1 cut(s)	1088
KroNI	GCCGGC	1 cut(s)	1090
Ksp22I	TGATCA	1 cut(s)	934
KspAI	GTTAAC	1 cut(s)	1888
Kzo9I	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
LguI	GCTCTTC	1 cut(s)	1038
LmnI	GCTCC	6 cut(s)	381, 466, 489, 758, 1677, 1768
Lsp1109I	GCAGC	8 cut(s)	372, 490, 493, 1259, 1586, 1814, 1847, 1989
LweI	GCATC	6 cut(s)	97, 509, 518, 1648, 1828, 1845
MaeI	CTAG	4 cut(s)	743, 1137, 1158, 2120
MaeII	ACGT	3 cut(s)	591, 728, 869
MaeIII	GTNAC	8 cut(s)	7, 266, 329, 448, 632, 724, 1109, 1555
MalI	GATC	7 cut(s)	299, 368, 936, 954, 1350, 1918, 2067
MbiI	CCGCTC	1 cut(s)	234
MboI	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
MflI	RGATCY	4 cut(s)	297, 952, 1916, 2065
MhlI	GDGCHC	3 cut(s)	378, 428, 1674
MluCI	AATT	7 cut(s)	83, 180, 907, 938, 946, 1054, 2215
MluI	ACGCGT	1 cut(s)	790
MlyI	GAGTC	1 cut(s)	1330
MmeI	TCCRAC	3 cut(s)	1054, 1479, 2064
Mph1103I	ATGCAT	3 cut(s)	1230, 1663, 2098
MroI	TCCGGA	2 cut(s)	1066, 1919
MroNI	GCCGGC	1 cut(s)	1088
MroXI	GAANNNNTTC	1 cut(s)	1593
MseI	TTAA	6 cut(s)	179, 1149, 1691, 1887, 1932, 1985
MslI	CAYNNNNRTG	1 cut(s)	854
MspA1I	CMGCKG	1 cut(s)	680
MspI	CCGG	9 cut(s)	554, 735, 814, 1067, 1089, 1902, 1920, 1974, 2208
MspR9I	CCNGG	5 cut(s)	554, 814, 815, 991, 1948
Mva1269I	GAATGC	2 cut(s)	1264, 2098
MvaI	CCWGG	2 cut(s)	991, 1948
MvnI	CGCG	2 cut(s)	740, 792
MwoI	GCNNNNNNNGC	9 cut(s)	231, 257, 382, 475, 506, 595, 1658, 1742, 1844
NaeI	GCCGGC	1 cut(s)	1090
NciI	CCSGG	3 cut(s)	554, 814, 815
NdeII	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
NgoMIV	GCCGGC	1 cut(s)	1088
NheI	GCTAGC	2 cut(s)	742, 2119
NlaIV	GGNNCC	9 cut(s)	405, 439, 531, 760, 954, 1183, 1770, 1918, 2067
NmeAIII	GCCGAG	1 cut(s)	1158
NmuCI	GTSAC	3 cut(s)	329, 448, 632
NsiI	ATGCAT	3 cut(s)	1230, 1663, 2098
NspI	RCATGY	4 cut(s)	659, 1024, 1656, 1794
PaeI	GCATGC	2 cut(s)	1656, 1794
PauI	GCGCGC	1 cut(s)	738
PciI	ACATGT	2 cut(s)	655, 1020
PciSI	GCTCTTC	1 cut(s)	1038
PcsI	WCGNNNNNNNCGW	1 cut(s)	725
PctI	GAATGC	2 cut(s)	1264, 2098
PdiI	GCCGGC	1 cut(s)	1090
PdmI	GAANNNNTTC	1 cut(s)	1593
PfeI	GAWTC	1 cut(s)	966
PflFI	GACNNNGTC	2 cut(s)	920, 1339
PflMI	CCANNNNNTGG	1 cut(s)	1619
Ple19I	CGATCG	1 cut(s)	369
PleI	GAGTC	1 cut(s)	1330
PmaCI	CACGTG	1 cut(s)	592
PmlI	CACGTG	1 cut(s)	592
PpsI	GAGTC	1 cut(s)	1330
Ppu21I	YACGTR	1 cut(s)	592
PscI	ACATGT	2 cut(s)	655, 1020
PsiI	TTATAA	1 cut(s)	1811
Psp124BI	GAGCTC	1 cut(s)	378
Psp6I	CCWGG	2 cut(s)	989, 1946
PspCI	CACGTG	1 cut(s)	592
PspFI	CCCAGC	1 cut(s)	1193
PspGI	CCWGG	2 cut(s)	989, 1946
PspN4I	GGNNCC	9 cut(s)	405, 439, 531, 760, 954, 1183, 1770, 1918, 2067
PspPI	GGNCC	4 cut(s)	530, 1622, 1899, 2210
PstI	CTGCAG	1 cut(s)	390
PstNI	CAGNNNCTG	1 cut(s)	1325
PsuI	RGATCY	4 cut(s)	297, 952, 1916, 2065
PsyI	GACNNNGTC	2 cut(s)	920, 1339
PteI	GCGCGC	1 cut(s)	738
PvuI	CGATCG	1 cut(s)	369
PvuII	CAGCTG	1 cut(s)	680
RsaI	GTAC	7 cut(s)	343, 1063, 1190, 1324, 1361, 1712, 1997
RsaNI	GTAC	7 cut(s)	342, 1062, 1189, 1323, 1360, 1711, 1996
RseI	CAYNNNNRTG	1 cut(s)	854
SacI	GAGCTC	1 cut(s)	378
SapI	GCTCTTC	1 cut(s)	1038
SaqAI	TTAA	6 cut(s)	179, 1149, 1691, 1887, 1932, 1985
Sau3AI	GATC	7 cut(s)	297, 366, 934, 952, 1348, 1916, 2065
Sau96I	GGNCC	4 cut(s)	530, 1622, 1899, 2210
SchI	GAGTC	1 cut(s)	1330
ScrFI	CCNGG	5 cut(s)	554, 814, 815, 991, 1948
SduI	GDGCHC	3 cut(s)	378, 428, 1674
SfaNI	GCATC	6 cut(s)	97, 509, 518, 1648, 1828, 1845
SfcI	CTRYAG	1 cut(s)	386
SinI	GGWCC	4 cut(s)	530, 1622, 1899, 2210
SmaI	CCCGGG	1 cut(s)	815
SmiMI	CAYNNNNRTG	1 cut(s)	854
SphI	GCATGC	2 cut(s)	1656, 1794
Sse9I	AATT	7 cut(s)	83, 180, 907, 938, 946, 1054, 2215
SsiI	CCGC	4 cut(s)	234, 257, 837, 2204
SspMI	CTAG	4 cut(s)	743, 1137, 1158, 2120
SstI	GAGCTC	1 cut(s)	378
StyD4I	CCNGG	5 cut(s)	552, 812, 813, 989, 1946
StyI	CCWWGG	1 cut(s)	576
TaaI	ACNGT	5 cut(s)	247, 266, 341, 1400, 1868
TaiI	ACGT	3 cut(s)	594, 731, 872
TaqI	TCGA	6 cut(s)	203, 369, 544, 1413, 1587, 2040
TaqII	GACCGA	1 cut(s)	2204
TasI	AATT	7 cut(s)	83, 180, 907, 938, 946, 1054, 2215
TatI	WGTACW	1 cut(s)	1995
TauI	GCSGC	3 cut(s)	260, 840, 2207
TfiI	GAWTC	1 cut(s)	966
Tru1I	TTAA	6 cut(s)	179, 1149, 1691, 1887, 1932, 1985
Tru9I	TTAA	6 cut(s)	179, 1149, 1691, 1887, 1932, 1985
TscAI	CASTG	8 cut(s)	502, 637, 1225, 1388, 1729, 1738, 1897, 2038
TseFI	GTSAC	3 cut(s)	329, 448, 632
TseI	GCWGC	8 cut(s)	385, 478, 481, 1272, 1574, 1802, 1835, 1977
Tsp45I	GTSAC	3 cut(s)	329, 448, 632
TspDTI	ATGAA	9 cut(s)	125, 147, 862, 1217, 1392, 1708, 2087, 2118, 2129
TspGWI	ACGGA	4 cut(s)	228, 255, 285, 1361
TspMI	CCCGGG	1 cut(s)	813
TspRI	CASTG	8 cut(s)	502, 637, 1225, 1388, 1729, 1738, 1897, 2038
Tth111I	GACNNNGTC	2 cut(s)	920, 1339
Van91I	CCANNNNNTGG	1 cut(s)	1619
VpaK11BI	GGWCC	4 cut(s)	530, 1622, 1899, 2210
XagI	CCTNNNNNAGG	2 cut(s)	458, 1945
XapI	RAATTY	3 cut(s)	907, 1054, 2215
XceI	RCATGY	4 cut(s)	659, 1024, 1656, 1794
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1721, 2076
XmaI	CCCGGG	1 cut(s)	813
XmiI	GTMKAC	2 cut(s)	1343, 1523
XmnI	GAANNNNTTC	1 cut(s)	1593
XspI	CTAG	4 cut(s)	743, 1137, 1158, 2120
Zsp2I	ATGCAT	3 cut(s)	1230, 1663, 2098

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.