Rh5AG354500

Phosphoenolpyruvate carboxykinase ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000550
Physical Location & Seq
Forward (+)
58653884 .. 58655403
1520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG354500.1

Sequence Viewer

Length: 609 bp
ATGCGAATTGTCTCTACCAGGCCTTATCATTTGTTGTTCATGCACAACATGTGCATCCGACTTACTCCTGAAGAGCTGGAGAATTTCGGTACTCCAGACTTCACTATATACAATGCCGGTCAGTTCCCGTGTAATCGTTACACTCATTACATGACATCCTCTACTAGCGTAGACATCAACCTTGCTAGCAAGGAAATGGTCATCCTTGGCGCCATGTACGCCGGGAAAATGAAGAAAGTGGTGGAAAATGTCACATTCGACGAGCATACCGGAGAGGTGGATTACAGTGACAAATCTATTACAGAAGTTAAACCTGGCGCTGAAGAGGGTGTGAAGGAGCCAGAGGCAACATTCTCGGCTTGCTCTGGTGCAGCACTTTTAATGTTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAGAACATGCAGAAGCATGGTGCAACTGCATGGCTGGTCAACACTGGTTGGTCTGGTCGAAGGTATGCAAATTCAAATAACATCATAAAAAACGTCATAGGACTGATCAATTTCCAAGACGGCATTGTTTGGGTTCAGGGTATAGCAGTTAGGTTCTATATGATACAGATAATGGTAAATGATGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map00710 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

202

Amino Acids

22.64

Weight (kDa)

6.06

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 2 - 81 2.4e-20 Phosphoenolpyruvate carboxykinase
PEPCK_ATP PF01293 106 - 166 5.9e-22 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000550)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37870 AT5G65690 AT5G65690 AT5G65690 AT5G65690
fragaria_vesca FvH4_1g18831 FvH4_1g18850 FvH4_1g18850 FvH4_1g24570 FvH4_4g10540 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_7g04810
malus_domestica MD01G1046200.v1.1 MD08G1207000.v1.1 MD15G1391200.v1.1 MD15G1405400.v1.1
prunus_persica Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.6G210900_v2.0.a1 Prupe.6G211000_v2.0.a1
pyrus_communis pycom01g07230 pycom08g17910 pycom15g26890 pycom16g22360
rosa_chinensis RchiOBHm_Chr2g0109711 RchiOBHm_Chr2g0109721 RchiOBHm_Chr5g0054131 RchiOBHm_Chr5g0054141 RchiOBHm_Chr5g0054151 RchiOBHm_Chr7g0233021
rosa_laevigata RLG00000001305 RLG00000017759 RLG00000017760 RLG00000030254 RLG00000030261 RLG00000030264
rosa_multiflora Rmu_sc0000507.1_g000005 Rmu_sc0000509.1_g000040 Rmu_sc0002076.1_g000010 Rmu_sc0006889.1_g000016 Rmu_sc0006889.1_g000018 Rmu_ssc0000018.1_g000034
rosa_roxburghii Rroxscaffold_2G00134000 Rroxscaffold_3G00227740 Rroxscaffold_4G00326310 Rroxscaffold_4G00326340
rosa_rugosa Rorug02G0162100 Rorug02G0162200 Rorug02G0162300 Rorug07G0277900.1
rosa_samantha Rh2AG214600 Rh2AG214700 Rh2BG224900 Rh2BG225000 Rh2BG225100 Rh2CG216800 Rh2CG216900 Rh2DG220100 Rh2DG220200 Rh5AG354400 Rh5AG354500 Rh5DG381200 Rh5DG381300 Rh7BG405700 Rh7CG452300 Rh7DG421600
rosa_wichuraiana Rw0G009650 Rw0G009660 Rw1G004940 Rw1G004970 Rw2G016580 Rw5G033400 Rw7G035700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 171
AcsI RAATTY 2 cut(s) 82, 487
AcuI CTGAAG 2 cut(s) 90, 342
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 2 cut(s) 91, 218
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 1 cut(s) 492
AjnI CCWGG 2 cut(s) 17, 313
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 1 cut(s) 16
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 371, 404
ApoI RAATTY 2 cut(s) 82, 487
AspLEI GCGC 2 cut(s) 212, 320
AsuC2I CCSGG 1 cut(s) 223
AsuNHI GCTAGC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 209
BbvI GCAGC 2 cut(s) 383, 416
BceAI ACGGC 1 cut(s) 553
BcgI CGANNNNNNTGC 2 cut(s) 336, 370
BciT130I CCWGG 2 cut(s) 19, 315
BclI TGATCA 1 cut(s) 522
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 2 cut(s) 165, 186
BfoI RGCGCY 2 cut(s) 213, 321
BisI GCNGC 2 cut(s) 372, 405
BlsI GCNGC 2 cut(s) 373, 406
Bme1390I CCNGG 3 cut(s) 19, 223, 315
BmiI GGNNCC 2 cut(s) 211, 339
BmrFI CCNGG 3 cut(s) 19, 223, 315
BmsI GCATC 2 cut(s) 63, 397
BmtI GCTAGC 1 cut(s) 189
BpmI CTGGAG 2 cut(s) 78, 98
BpuMI CCSGG 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 466
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 2 cut(s) 19, 315
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 4 cut(s) 54, 155, 201, 388
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 466
BseXI GCAGC 2 cut(s) 383, 416
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 117, 222, 270
BsmAI GTCTC 1 cut(s) 16
BsnI GGCC 1 cut(s) 22
Bsp143I GATC 1 cut(s) 522
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 211, 339
BspOI GCTAGC 1 cut(s) 189
BspQI GCTCTTC 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 466
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 522
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 2 cut(s) 19, 315
Bst4CI ACNGT 1 cut(s) 287
Bst6I CTCTTC 2 cut(s) 66, 318
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 187, 361, 414
BstDEI CTNAG 1 cut(s) 417
BstF5I GGATG 4 cut(s) 54, 155, 201, 388
BstH2I RGCGCY 2 cut(s) 213, 321
BstHHI GCGC 2 cut(s) 212, 320
BstKTI GATC 1 cut(s) 525
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 1 cut(s) 522
BstMWI GCNNNNNNNGC 2 cut(s) 218, 413
BstNI CCWGG 2 cut(s) 19, 315
BstNSI RCATGY 2 cut(s) 52, 427
BstSCI CCNGG 3 cut(s) 17, 221, 313
BstV1I GCAGC 2 cut(s) 383, 416
BsuRI GGCC 1 cut(s) 22
BtsCI GGATG 4 cut(s) 54, 155, 201, 388
BtsIMutI CAGTG 2 cut(s) 292, 459
Cac8I GCNNGC 3 cut(s) 187, 361, 414
CfoI GCGC 2 cut(s) 212, 320
Cfr10I RCCGGY 1 cut(s) 116
Csp6I GTAC 2 cut(s) 90, 217
CviAII CATG 7 cut(s) 40, 49, 151, 214, 424, 434, 447
CviJI RGCY 6 cut(s) 22, 76, 340, 359, 416, 451
CviKI_1 RGCY 6 cut(s) 22, 76, 340, 359, 416, 451
CviQI GTAC 2 cut(s) 90, 217
DdeI CTNAG 1 cut(s) 417
DinI GGCGCC 1 cut(s) 211
DpnI GATC 1 cut(s) 524
DpnII GATC 1 cut(s) 522
Eam1104I CTCTTC 2 cut(s) 66, 318
EarI CTCTTC 2 cut(s) 66, 318
Eco130I CCWWGG 1 cut(s) 205
Eco147I AGGCCT 1 cut(s) 22
Eco57I CTGAAG 2 cut(s) 90, 342
EcoRII CCWGG 2 cut(s) 17, 313
EcoT14I CCWWGG 1 cut(s) 205
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 7 cut(s) 43, 52, 154, 217, 427, 437, 450
FatI CATG 7 cut(s) 39, 48, 150, 213, 423, 433, 446
FbaI TGATCA 1 cut(s) 522
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 372, 405
FokI GGATG 4 cut(s) 41, 142, 188, 375
Fsp4HI GCNGC 2 cut(s) 372, 405
FspBI CTAG 2 cut(s) 165, 186
GlaI GCGC 2 cut(s) 211, 319
GluI GCNGC 2 cut(s) 372, 405
GsuI CTGGAG 2 cut(s) 78, 98
HaeII RGCGCY 2 cut(s) 213, 321
HaeIII GGCC 1 cut(s) 22
HapII CCGG 3 cut(s) 117, 222, 270
HhaI GCGC 2 cut(s) 212, 320
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 7 cut(s) 43, 52, 154, 217, 427, 437, 450
Hin6I GCGC 2 cut(s) 210, 318
HinP1I GCGC 2 cut(s) 210, 318
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HpaII CCGG 3 cut(s) 117, 222, 270
Hpy166II GTNNAC 2 cut(s) 172, 457
Hpy188I TCNGA 1 cut(s) 59
Hpy188III TCNNGA 2 cut(s) 68, 95
Hpy8I GTNNAC 2 cut(s) 172, 457
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 328, 471
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 9 cut(s) 43, 54, 371, 388, 404, 427, 440, 446, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 413
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 1 cut(s) 510
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 7 cut(s) 43, 52, 154, 217, 427, 437, 450
HspAI GCGC 2 cut(s) 210, 318
KasI GGCGCC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 1 cut(s) 522
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 2 cut(s) 383, 416
LweI GCATC 2 cut(s) 63, 397
MaeI CTAG 2 cut(s) 165, 186
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 3 cut(s) 137, 250, 287
MalI GATC 1 cut(s) 524
MboI GATC 1 cut(s) 522
MboII GAAGA 3 cut(s) 83, 244, 335
MluCI AATT 4 cut(s) 6, 82, 487, 526
Mly113I GGCGCC 1 cut(s) 210
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 4 cut(s) 169, 268, 319, 337
MseI TTAA 2 cut(s) 309, 380
MspI CCGG 3 cut(s) 117, 222, 270
MspR9I CCNGG 3 cut(s) 19, 223, 315
MvaI CCWGG 2 cut(s) 19, 315
MwoI GCNNNNNNNGC 2 cut(s) 218, 413
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 1 cut(s) 223
NdeII GATC 1 cut(s) 522
NheI GCTAGC 1 cut(s) 185
NlaIII CATG 7 cut(s) 43, 52, 154, 217, 427, 437, 450
NlaIV GGNNCC 2 cut(s) 211, 339
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 2 cut(s) 250, 287
NspI RCATGY 2 cut(s) 52, 427
PceI AGGCCT 1 cut(s) 22
PciI ACATGT 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 66
PkrI GCNGC 2 cut(s) 373, 406
PluTI GGCGCC 1 cut(s) 213
PscI ACATGT 1 cut(s) 48
Psp6I CCWGG 2 cut(s) 17, 313
PspGI CCWGG 2 cut(s) 17, 313
PspN4I GGNNCC 2 cut(s) 211, 339
RsaI GTAC 2 cut(s) 91, 218
RsaNI GTAC 2 cut(s) 90, 217
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 2 cut(s) 309, 380
SatI GCNGC 2 cut(s) 372, 405
Sau3AI GATC 1 cut(s) 522
ScrFI CCNGG 3 cut(s) 19, 223, 315
SetI ASST 7 cut(s) 78, 183, 279, 316, 482, 513, 572
SfaNI GCATC 2 cut(s) 63, 397
SfoI GGCGCC 1 cut(s) 211
Sse9I AATT 4 cut(s) 6, 82, 487, 526
SseBI AGGCCT 1 cut(s) 22
SspDI GGCGCC 1 cut(s) 209
SspMI CTAG 2 cut(s) 165, 186
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 3 cut(s) 17, 221, 313
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 1 cut(s) 513
TaqI TCGA 2 cut(s) 258, 475
TasI AATT 4 cut(s) 6, 82, 487, 526
Tru1I TTAA 2 cut(s) 309, 380
Tru9I TTAA 2 cut(s) 309, 380
TscAI CASTG 2 cut(s) 292, 466
TseFI GTSAC 2 cut(s) 250, 287
TseI GCWGC 2 cut(s) 371, 404
Tsp45I GTSAC 2 cut(s) 250, 287
TspDTI ATGAA 2 cut(s) 28, 245
TspRI CASTG 2 cut(s) 292, 466
XapI RAATTY 2 cut(s) 82, 487
XceI RCATGY 2 cut(s) 52, 427
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 2 cut(s) 165, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.