RchiOBHm_Chr5g0054151

Phosphoenolpyruvate carboxykinase ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000550
Physical Location & Seq
Forward (+)
56609335 .. 56612041
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33136

Sequence Viewer

Length: 522 bp
ATGTGGAAAGATGGAGATGTTGCCCTCTTCTTCGGATTGTCAGGCACCGGAAAGACGACTCAATCTACGGATCACAATAGGAACTTAATTGGTGATGATGAACACTGTTGGAATGAGAATGGTCAAGTTAAACCTGGCGCTGAAGAGGGTGTGAAGGAGCCACGGGCAACATTCTCGGCTTGCTCTGGTGCAGCATATTTAATGTTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAGAAGATGCAGAAGCATGGTGCAACTGCATGGCTGGTCAACACTGGTTGGTCTGGTGGAAGGTATGCAAATTCAAATGACATCATAAAAAACGTCATAGGACTGATCAATTTCCAAAACGGCATTGTTTGGATTCAGGGTATAGCAGTTAGGTTCTATATGATAGAGATAATGGTAAATGATGTAGTTCGAGATATAACCAGAAGATCAACACCAAAAAGAGCAGCAGATGATATAAGCCTCTATCTTTGTTCCCGGCAGCTATGGATCAGGAAGCCGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map00710 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

173

Amino Acids

19.36

Weight (kDa)

8.79

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 3 - 41 3.5e-17 Phosphoenolpyruvate carboxykinase
PEPCK_ATP PF01293 46 - 104 1.4e-23 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000550)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37870 AT5G65690 AT5G65690 AT5G65690 AT5G65690
fragaria_vesca FvH4_1g18831 FvH4_1g18850 FvH4_1g18850 FvH4_1g24570 FvH4_4g10540 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_7g04810
malus_domestica MD01G1046200.v1.1 MD08G1207000.v1.1 MD15G1391200.v1.1 MD15G1405400.v1.1
prunus_persica Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.6G210900_v2.0.a1 Prupe.6G211000_v2.0.a1
pyrus_communis pycom01g07230 pycom08g17910 pycom15g26890 pycom16g22360
rosa_chinensis RchiOBHm_Chr2g0109711 RchiOBHm_Chr2g0109721 RchiOBHm_Chr5g0054131 RchiOBHm_Chr5g0054141 RchiOBHm_Chr5g0054151 RchiOBHm_Chr7g0233021
rosa_laevigata RLG00000001305 RLG00000017759 RLG00000017760 RLG00000030254 RLG00000030261 RLG00000030264
rosa_multiflora Rmu_sc0000507.1_g000005 Rmu_sc0000509.1_g000040 Rmu_sc0002076.1_g000010 Rmu_sc0006889.1_g000016 Rmu_sc0006889.1_g000018 Rmu_ssc0000018.1_g000034
rosa_roxburghii Rroxscaffold_2G00134000 Rroxscaffold_3G00227740 Rroxscaffold_4G00326310 Rroxscaffold_4G00326340
rosa_rugosa Rorug02G0162100 Rorug02G0162200 Rorug02G0162300 Rorug07G0277900.1
rosa_samantha Rh2AG214600 Rh2AG214700 Rh2BG224900 Rh2BG225000 Rh2BG225100 Rh2CG216800 Rh2CG216900 Rh2DG220100 Rh2DG220200 Rh5AG354400 Rh5AG354500 Rh5DG381200 Rh5DG381300 Rh7BG405700 Rh7CG452300 Rh7DG421600
rosa_wichuraiana Rw0G009650 Rw0G009660 Rw1G004940 Rw1G004970 Rw2G016580 Rw5G033400 Rw7G035700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 44
AciI CCGC 1 cut(s) 515
AclWI GGATC 2 cut(s) 78, 512
AcsI RAATTY 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 162
AgsI TTSAA 1 cut(s) 312
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 1 cut(s) 499
AluI AGCT 1 cut(s) 499
AlwI GGATC 2 cut(s) 78, 512
ApeKI GCWGC 4 cut(s) 191, 224, 461, 496
ApoI RAATTY 1 cut(s) 307
AspLEI GCGC 1 cut(s) 140
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 1 cut(s) 104
BanI GGYRCC 1 cut(s) 44
BbvI GCAGC 4 cut(s) 203, 236, 473, 508
BccI CCATC 1 cut(s) 5
BceAI ACGGC 1 cut(s) 373
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 493
BfoI RGCGCY 1 cut(s) 141
BisI GCNGC 5 cut(s) 192, 225, 462, 497, 515
BlsI GCNGC 5 cut(s) 193, 226, 463, 498, 516
Bme1390I CCNGG 2 cut(s) 135, 493
BmiI GGNNCC 2 cut(s) 46, 159
BmrFI CCNGG 2 cut(s) 135, 493
BmsI GCATC 2 cut(s) 217, 234
BpuMI CCSGG 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 286
BseXI GCAGC 4 cut(s) 203, 236, 473, 508
BsgI GTGCAG 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 44
BsiSI CCGG 2 cut(s) 48, 493
Bsp143I GATC 4 cut(s) 70, 342, 443, 504
BspACI CCGC 1 cut(s) 515
BspCNI CTCAG 1 cut(s) 229
BspLI GGNNCC 2 cut(s) 46, 159
BspPI GGATC 2 cut(s) 78, 512
BspT107I GGYRCC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 4 cut(s) 70, 342, 443, 504
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 2 cut(s) 32, 138
BstC8I GCNNGC 2 cut(s) 181, 234
BstDEI CTNAG 1 cut(s) 237
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 73, 345, 446, 507
BstMBI GATC 4 cut(s) 70, 342, 443, 504
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 133, 491
BstV1I GCAGC 4 cut(s) 203, 236, 473, 508
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 103, 279
Cac8I GCNNGC 2 cut(s) 181, 234
CfoI GCGC 1 cut(s) 140
CviAII CATG 2 cut(s) 254, 267
CviJI RGCY 7 cut(s) 160, 179, 236, 271, 477, 499, 514
CviKI_1 RGCY 7 cut(s) 160, 179, 236, 271, 477, 499, 514
DdeI CTNAG 1 cut(s) 237
DpnI GATC 4 cut(s) 72, 344, 445, 506
DpnII GATC 4 cut(s) 70, 342, 443, 504
Eam1104I CTCTTC 2 cut(s) 32, 138
EarI CTCTTC 2 cut(s) 32, 138
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 133
FaeI CATG 2 cut(s) 257, 270
FatI CATG 2 cut(s) 253, 266
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 5 cut(s) 192, 225, 462, 497, 515
FokI GGATG 1 cut(s) 195
Fsp4HI GCNGC 5 cut(s) 192, 225, 462, 497, 515
GlaI GCGC 1 cut(s) 139
GluI GCNGC 5 cut(s) 192, 225, 462, 497, 515
HaeII RGCGCY 1 cut(s) 141
HapII CCGG 2 cut(s) 48, 493
HhaI GCGC 1 cut(s) 140
Hin1II CATG 2 cut(s) 257, 270
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 2 cut(s) 58, 370
HpaII CCGG 2 cut(s) 48, 493
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 1 cut(s) 35
Hpy188III TCNNGA 2 cut(s) 428, 508
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 148, 291
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 7 cut(s) 191, 208, 224, 247, 260, 266, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 237
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 257, 270
HspAI GCGC 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 4 cut(s) 70, 342, 443, 504
LmnI GCTCC 1 cut(s) 157
Lsp1109I GCAGC 4 cut(s) 203, 236, 473, 508
LweI GCATC 2 cut(s) 217, 234
MaeII ACGT 1 cut(s) 330
MalI GATC 4 cut(s) 72, 344, 445, 506
MboI GATC 4 cut(s) 70, 342, 443, 504
MboII GAAGA 5 cut(s) 19, 22, 155, 253, 453
MluCI AATT 3 cut(s) 87, 307, 346
MlyI GAGTC 1 cut(s) 52
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 3 cut(s) 35, 139, 488
MseI TTAA 4 cut(s) 86, 129, 200, 520
MspI CCGG 2 cut(s) 48, 493
MspR9I CCNGG 2 cut(s) 135, 493
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 233
NciI CCSGG 1 cut(s) 493
NdeII GATC 4 cut(s) 70, 342, 443, 504
NlaIII CATG 2 cut(s) 257, 270
NlaIV GGNNCC 2 cut(s) 46, 159
NmeAIII GCCGAG 1 cut(s) 155
PfeI GAWTC 1 cut(s) 370
PkrI GCNGC 5 cut(s) 193, 226, 463, 498, 516
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 46, 159
SaqAI TTAA 4 cut(s) 86, 129, 200, 520
SatI GCNGC 5 cut(s) 192, 225, 462, 497, 515
Sau3AI GATC 4 cut(s) 70, 342, 443, 504
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 2 cut(s) 135, 493
SetI ASST 5 cut(s) 136, 302, 333, 392, 501
SfaNI GCATC 2 cut(s) 217, 234
Sse9I AATT 3 cut(s) 87, 307, 346
SsiI CCGC 1 cut(s) 515
StyD4I CCNGG 2 cut(s) 133, 491
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 1 cut(s) 333
TaqI TCGA 1 cut(s) 427
TasI AATT 3 cut(s) 87, 307, 346
TauI GCSGC 1 cut(s) 517
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 4 cut(s) 86, 129, 200, 520
Tru9I TTAA 4 cut(s) 86, 129, 200, 520
TscAI CASTG 2 cut(s) 110, 286
TseI GCWGC 4 cut(s) 191, 224, 461, 496
TspDTI ATGAA 1 cut(s) 114
TspGWI ACGGA 1 cut(s) 83
TspRI CASTG 2 cut(s) 110, 286
XapI RAATTY 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.