Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000550

Physical Location & Seq

Genomic Coordinates Reverse (-)

26875162 .. 26881676

Gene Structure 6515 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1207000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGGGACGCCATGGACTGCCGAAAATCCAAACCCAGCATGAGAAGTCCTCGGCCGAGGTTTGCCACGACGACAGCACAACGCCGGTGAAGGCCAAGACGATCGACGAGCTCCACTCTCTTCAGAAGAAGAAGTCGCAACCCACCACCCCCATCACCGGATCGCAGGGGGCTTTTACCCCCACCATGTCTGAAGAGGATCGCCACAGGCAGCAGCTCCAGTCCATCAGTGCCTCATTGGCGTCACTGACGAGGGAGACGGGGCCGAAGGTGGTGAAGGGGGACCCGAAGTCAGATGGGCAGAAGGCGACGCATGTTGAACATCATCTTTACGACGCTTCTCTCTTTGGCGTCAGTGATAGTGCCCTCAAGTTTACCCATATCCTTTACAATCTCTCTCCTGCTGAACTATATGAGCAAGCTATAAAGTATGAGAAAGGTTCGTTTATAACGGCATCGGGTGCGTTGGCCACTTTATCTGGGGCAAAAACTGGGCGTTCTCCAAGAGACAAACGAGTTGTCAAAGATGAAGAAACTGAAAACGAGCTTTGGTGGGGAAAGGGCTCACCCAACATTGAAATGGACGAGCATACTTTCTTAGTGAACAGAGAAAGAGCTGTAGATTACTTGAATTCCTTGGACAAGGTTTTTGTGAATGATCAGTTTCTTAACTGGGATCCAGAACACCGAATCAAAGTTCGGATTGTATCTGCGAGAGCTTACCACTCCCTCTTTATGCATAACATGTGCATCCGCCCTACTCCTGAAGAGCTGGAGGATTTCAGTACTCCAGACTTCACAATATACAACGCCGGGCAGTTTCCATGTAACCGTTTCACTCACCACATGACGTCCTCTACAAGCGTAGACCTCAATCTTTCTAGGAGGGAAATGGTCATCCTTGGCACCCAGTATGCTGGAGAAATGAAGAAAGGCCTGTTCAGTGTCATGCACTATCTCATGCCTAAGCGTCAAATCCTCTCCCTCCACTCTGGGTGCAATATGGGGAAAGGCGGCGATGTTGCCCTTTTCTTTGGCTTATCTGGCACTGGGAAGACGACTCTGTCTACAGACCATAATCGGTATCTAATTGGAGATGATGAGCACTGCTGGAGCAACAATGGTGTGTCAAACATTGAAGGTGGTTGCTATGCCAAGTGCATTGATTTGTCAAAGGACAAGGAACCAGATATTTGGAACGCTATCAAATTCGGGACTGTTTTGGAGAATGTTGTGTTTGATGAGTATAATCGAGAAGTTGATTACTCAGAGAAATCTGTTACTGAAAACACCCGGGCAGCATATCCAATAGAGTATATACCGAATGCTAAAATACCGTGTGTTGGCCCCCATCCGAAAAATGTCATCCTCCTGGCATGTGATGCATTTGGTGTGCTTCCACCAGTGAGCAAGCTGAGCTTGGCTCAAACTATGTACCATTTCATCAGCGGTTACACTGCTTTGGTGGCTGGAACAGAAGATGGTATTAAGGAGCCACAAGCTACATTCTCAGCATGTTTCGGTGCAGCGTTTATTATGATGCATCCGACAAGGTATGCAGCAATGTTGGCTGAAAAGATGCAGAAGCACGGAGCCACAGGGTGGCTTGTTAACACAGGGTGGTCTGCAGGAAGGTATGGAACAGGTAACCGTATCAAATTAGCCTATACCAGGAAGATTATCGATGCGATCCACTCGGGGGACCTTCTGAATGCAACCTACACAAAGACTGAAGTTTTCGGTCTTGAGATTCCTACAGAGATTGAGGGTGTACCTTCAGAAATTCTGGACCCAATAAACACCTGGTCAGACAAGAAAGCCTACAAAGACACACTACTTCATCTGGGTGGTCTGTTCAAGAAGAACTTCGAGGTGTTCATGAATCACAAGATCGGAAAGGACAACAAGCTGACCGGGGAAATTCTTGCAGCGGGACCAAATTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004611 GO:0004612 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006094 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0008150 GO:0008152 GO:0009058 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0016020 GO:0016036 GO:0016051 GO:0016829 GO:0016830 GO:0016831 GO:0019318 GO:0019319 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0033554 GO:0042221 GO:0042594 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046364 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070013 GO:0071496 GO:0071704 GO:0098542 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PEPCK_ATP

Protein Analysis

648

Amino Acids

71.98

Weight (kDa)

6.52

Isoelectric Point (pI)

37.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPCK_ATP	PF01293	126 - 593	4e-213	Phosphoenolpyruvate carboxykinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37870 AT5G65690 AT5G65690 AT5G65690 AT5G65690
fragaria_vesca	FvH4_1g18831 FvH4_1g18850 FvH4_1g18850 FvH4_1g24570 FvH4_4g10540 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_7g04810
malus_domestica	MD01G1046200.v1.1 MD08G1207000.v1.1 MD15G1391200.v1.1 MD15G1405400.v1.1
prunus_persica	Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.6G210900_v2.0.a1 Prupe.6G211000_v2.0.a1
pyrus_communis	pycom01g07230 pycom08g17910 pycom15g26890 pycom16g22360
rosa_chinensis	RchiOBHm_Chr2g0109711 RchiOBHm_Chr2g0109721 RchiOBHm_Chr5g0054131 RchiOBHm_Chr5g0054141 RchiOBHm_Chr5g0054151 RchiOBHm_Chr7g0233021
rosa_laevigata	RLG00000001305 RLG00000017759 RLG00000017760 RLG00000030254 RLG00000030261 RLG00000030264
rosa_multiflora	Rmu_sc0000507.1_g000005 Rmu_sc0000509.1_g000040 Rmu_sc0002076.1_g000010 Rmu_sc0006889.1_g000016 Rmu_sc0006889.1_g000018 Rmu_ssc0000018.1_g000034
rosa_roxburghii	Rroxscaffold_2G00134000 Rroxscaffold_3G00227740 Rroxscaffold_4G00326310 Rroxscaffold_4G00326340
rosa_rugosa	Rorug02G0162100 Rorug02G0162200 Rorug02G0162300 Rorug07G0277900.1
rosa_samantha	Rh2AG214600 Rh2AG214700 Rh2BG224900 Rh2BG225000 Rh2BG225100 Rh2CG216800 Rh2CG216900 Rh2DG220100 Rh2DG220200 Rh5AG354400 Rh5AG354500 Rh5DG381200 Rh5DG381300 Rh7BG405700 Rh7CG452300 Rh7DG421600
rosa_wichuraiana	Rw0G009650 Rw0G009660 Rw1G004940 Rw1G004970 Rw2G016580 Rw5G033400 Rw7G035700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	446
AasI	GACNNNNNNGTC	1 cut(s)	1060
AatII	GACGTC	1 cut(s)	851
AccB1I	GGYRCC	1 cut(s)	902
AccB7I	CCANNNNNTGG	1 cut(s)	1599
AccI	GTMKAC	2 cut(s)	864, 1064
AciI	CCGC	4 cut(s)	751, 1011, 1446, 1928
AclWI	GGATC	5 cut(s)	166, 204, 668, 681, 1681
AcoI	YGGCCR	2 cut(s)	51, 465
AcsI	RAATTY	5 cut(s)	628, 1205, 1779, 1917, 1936
AcuI	CTGAAG	5 cut(s)	104, 210, 783, 1749, 1758
AcyI	GRCGYC	4 cut(s)	7, 239, 348, 848
AdeI	CACNNNGTG	2 cut(s)	1599, 1617
AfaI	GTAC	3 cut(s)	784, 1433, 1770
AfiI	CCNNNNNNNGG	5 cut(s)	155, 1340, 1599, 1668, 1696
AflIII	ACRYGT	1 cut(s)	741
AgsI	TTSAA	5 cut(s)	317, 575, 628, 1136, 1855
AhdI	GACNNNNNGTC	1 cut(s)	286
AjnI	CCWGG	3 cut(s)	1368, 1667, 1799
Alw21I	GWGCWC	2 cut(s)	111, 1104
Alw26I	GTCTC	2 cut(s)	248, 498
AlwI	GGATC	5 cut(s)	166, 204, 668, 681, 1681
Ama87I	CYCGRG	2 cut(s)	1290, 1693
AoxI	GGCC	6 cut(s)	51, 90, 260, 465, 931, 1342
ApeKI	GCWGC	6 cut(s)	208, 211, 1295, 1523, 1556, 1925
ApoI	RAATTY	5 cut(s)	628, 1205, 1779, 1917, 1936
ArsI	GACNNNNNNTTYG	5 cut(s)	501, 533, 629, 661, 1923
Asp700I	GAANNNNTTC	1 cut(s)	1862
AspS9I	GGNCC	6 cut(s)	260, 280, 1343, 1699, 1786, 1931
AsuC2I	CCSGG	4 cut(s)	811, 1291, 1292, 1912
AsuHPI	GGTGA	5 cut(s)	97, 145, 283, 555, 830
AvaI	CYCGRG	2 cut(s)	1290, 1693
AvaII	GGWCC	4 cut(s)	280, 1699, 1786, 1931
BaeGI	GKGCMC	1 cut(s)	364
BalI	TGGCCA	1 cut(s)	467
BamHI	GGATCC	1 cut(s)	673
BanI	GGYRCC	1 cut(s)	902
BanII	GRGCYC	2 cut(s)	111, 563
BbsI	GAAGAC	1 cut(s)	1058
Bbv12I	GWGCWC	2 cut(s)	111, 1104
BbvI	GCAGC	6 cut(s)	220, 223, 1307, 1535, 1568, 1937
BccI	CCATC	5 cut(s)	158, 230, 287, 1356, 1472
BceAI	ACGGC	1 cut(s)	465
BciT130I	CCWGG	3 cut(s)	1370, 1669, 1801
BclI	TGATCA	1 cut(s)	655
BcnI	CCSGG	4 cut(s)	811, 1291, 1292, 1912
BcoDI	GTCTC	2 cut(s)	248, 498
BfaI	CTAG	1 cut(s)	879
BfmI	CTRYAG	4 cut(s)	615, 1065, 1623, 1752
BglI	GCCNNNNNGGC	1 cut(s)	236
BisI	GCNGC	7 cut(s)	209, 212, 1012, 1296, 1524, 1557, 1926
BlpI	GCTNAGC	1 cut(s)	1412
BlsI	GCNGC	7 cut(s)	210, 213, 1013, 1297, 1525, 1558, 1927
BmcAI	AGTACT	1 cut(s)	784
Bme1390I	CCNGG	7 cut(s)	811, 1291, 1292, 1370, 1669, 1801, 1912
Bme18I	GGWCC	4 cut(s)	280, 1699, 1786, 1931
BmeRI	GACNNNNNGTC	1 cut(s)	286
BmeT110I	CYCGRG	2 cut(s)	1290, 1693
BmgT120I	GGNCC	6 cut(s)	260, 280, 1343, 1699, 1786, 1931
BmrFI	CCNGG	7 cut(s)	811, 1291, 1292, 1370, 1669, 1801, 1912
BmrI	ACTGGG	4 cut(s)	498, 679, 901, 1056
BmsI	GCATC	7 cut(s)	461, 756, 1369, 1527, 1549, 1566, 1672
BmuI	ACTGGG	4 cut(s)	498, 679, 901, 1056
BpiI	GAAGAC	1 cut(s)	1058
BplI	GAGNNNNNCTC	6 cut(s)	32, 64, 98, 130, 1405, 1437
BpmI	CTGGAG	5 cut(s)	200, 771, 791, 936, 1129
Bpu10I	CCTNAGC	1 cut(s)	963
Bpu1102I	GCTNAGC	1 cut(s)	1412
BpuEI	CTTGAG	2 cut(s)	350, 1763
BpuMI	CCSGG	4 cut(s)	811, 1291, 1292, 1912
Bsa29I	ATCGAT	1 cut(s)	1680
BsaHI	GRCGYC	4 cut(s)	7, 239, 348, 848
BsaJI	CCNNGG	7 cut(s)	10, 48, 54, 633, 898, 1290, 1911
BsaWI	WCCGGW	1 cut(s)	155
Bsc4I	CCNNNNNNNGG	5 cut(s)	155, 1340, 1599, 1668, 1696
Bse118I	RCCGGY	1 cut(s)	82
Bse1I	ACTGG	6 cut(s)	217, 493, 674, 907, 1051, 1400
Bse3DI	GCAATG	1 cut(s)	1566
BseBI	CCWGG	3 cut(s)	1370, 1669, 1801
BseCI	ATCGAT	1 cut(s)	1680
BseDI	CCNNGG	7 cut(s)	10, 48, 54, 633, 898, 1290, 1911
BseGI	GGATG	5 cut(s)	747, 894, 1348, 1362, 1540
BseLI	CCNNNNNNNGG	5 cut(s)	155, 1340, 1599, 1668, 1696
BseMI	GCAATG	1 cut(s)	1566
BseMII	CTCAG	3 cut(s)	1278, 1403, 1521
BseNI	ACTGG	6 cut(s)	217, 493, 674, 907, 1051, 1400
BseSI	GKGCMC	1 cut(s)	364
BseX3I	CGGCCG	1 cut(s)	51
BseXI	GCAGC	6 cut(s)	220, 223, 1307, 1535, 1568, 1937
BseYI	CCCAGC	1 cut(s)	33
BsgI	GTGCAG	1 cut(s)	1542
Bsh1285I	CGRYCG	2 cut(s)	54, 102
BshFI	GGCC	6 cut(s)	53, 92, 262, 467, 933, 1344
BshNI	GGYRCC	1 cut(s)	902
BshVI	ATCGAT	1 cut(s)	1680
BsiEI	CGRYCG	2 cut(s)	54, 102
BsiHKAI	GWGCWC	2 cut(s)	111, 1104
BsiHKCI	CYCGRG	2 cut(s)	1290, 1693
BsiSI	CCGG	5 cut(s)	83, 156, 810, 1291, 1911
BslFI	GGGAC	4 cut(s)	18, 293, 1225, 1712
BslI	CCNNNNNNNGG	5 cut(s)	155, 1340, 1599, 1668, 1696
BsmAI	GTCTC	2 cut(s)	248, 498
BsmBI	CGTCTC	1 cut(s)	248
BsmFI	GGGAC	4 cut(s)	18, 293, 1225, 1712
BsmI	GAATGC	2 cut(s)	1327, 1714
BsnI	GGCC	6 cut(s)	53, 92, 262, 467, 933, 1344
BsoBI	CYCGRG	2 cut(s)	1290, 1693
Bsp1286I	GDGCHC	4 cut(s)	111, 364, 563, 1104
Bsp143I	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
Bsp1720I	GCTNAGC	1 cut(s)	1412
Bsp19I	CCATGG	1 cut(s)	10
BspACI	CCGC	4 cut(s)	751, 1011, 1446, 1928
BspANI	GGCC	6 cut(s)	53, 92, 262, 467, 933, 1344
BspCNI	CTCAG	3 cut(s)	1277, 1404, 1520
BspDI	ATCGAT	1 cut(s)	1680
BspHI	TCATGA	1 cut(s)	1875
BspMAI	CTGCAG	1 cut(s)	1627
BspPI	GGATC	5 cut(s)	166, 204, 668, 681, 1681
BspQI	GCTCTTC	1 cut(s)	759
BspT107I	GGYRCC	1 cut(s)	902
BsrDI	GCAATG	1 cut(s)	1566
BsrFI	RCCGGY	1 cut(s)	82
BsrI	ACTGG	6 cut(s)	217, 493, 674, 907, 1051, 1400
BssAI	RCCGGY	1 cut(s)	82
BssECI	CCNNGG	7 cut(s)	10, 48, 54, 633, 898, 1290, 1911
BssMI	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
BssNI	GRCGYC	4 cut(s)	7, 239, 348, 848
BssT1I	CCWWGG	3 cut(s)	10, 633, 898
Bst2UI	CCWGG	3 cut(s)	1370, 1669, 1801
Bst4CI	ACNGT	4 cut(s)	830, 1216, 1335, 1649
Bst6I	CTCTTC	3 cut(s)	123, 186, 759
BstACI	GRCGYC	4 cut(s)	7, 239, 348, 848
BstAPI	GCANNNNNTGC	2 cut(s)	458, 1379
BstC8I	GCNNGC	2 cut(s)	417, 1409
BstDEI	CTNAG	5 cut(s)	595, 963, 1264, 1412, 1507
BstDSI	CCRYGG	1 cut(s)	10
BstEII	GGTNACC	1 cut(s)	1643
BstENI	CCTNNNNNAGG	1 cut(s)	1666
BstF5I	GGATG	5 cut(s)	747, 894, 1348, 1362, 1540
BstKTI	GATC	7 cut(s)	102, 161, 199, 658, 676, 1689, 1890
BstMAI	GTCTC	2 cut(s)	248, 498
BstMBI	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
BstMCI	CGRYCG	2 cut(s)	54, 102
BstMWI	GCNNNNNNNGC	7 cut(s)	236, 458, 1041, 1379, 1413, 1463, 1565
BstNI	CCWGG	3 cut(s)	1370, 1669, 1801
BstNSI	RCATGY	4 cut(s)	314, 745, 1377, 1515
BstPI	GGTNACC	1 cut(s)	1643
BstSCI	CCNGG	7 cut(s)	809, 1289, 1290, 1368, 1667, 1799, 1910
BstSFI	CTRYAG	4 cut(s)	615, 1065, 1623, 1752
BstSLI	GKGCMC	1 cut(s)	364
BstV1I	GCAGC	6 cut(s)	220, 223, 1307, 1535, 1568, 1937
BstV2I	GAAGAC	1 cut(s)	1058
BstX2I	RGATCY	1 cut(s)	673
BstXI	CCANNNNNNTGG	2 cut(s)	914, 1191
BstYI	RGATCY	1 cut(s)	673
BstZI	CGGCCG	1 cut(s)	51
Bsu15I	ATCGAT	1 cut(s)	1680
BsuRI	GGCC	6 cut(s)	53, 92, 262, 467, 933, 1344
BsuTUI	ATCGAT	1 cut(s)	1680
BtgI	CCRYGG	1 cut(s)	10
BtgZI	GCGATG	1 cut(s)	1029
BtsCI	GGATG	5 cut(s)	747, 894, 1348, 1362, 1540
BtsI	GCAGTG	2 cut(s)	1102, 1452
BtsIMutI	CAGTG	8 cut(s)	232, 242, 358, 946, 1044, 1102, 1407, 1452
Cac8I	GCNNGC	2 cut(s)	417, 1409
CciI	TCATGA	1 cut(s)	1875
Cfr10I	RCCGGY	1 cut(s)	82
Cfr13I	GGNCC	6 cut(s)	260, 280, 1343, 1699, 1786, 1931
Cfr9I	CCCGGG	1 cut(s)	1290
ClaI	ATCGAT	1 cut(s)	1680
CseI	GACGC	6 cut(s)	15, 228, 316, 337, 341, 956
CsiI	ACCWGGT	1 cut(s)	1799
Csp6I	GTAC	3 cut(s)	783, 1432, 1769
CviQI	GTAC	3 cut(s)	783, 1432, 1769
DdeI	CTNAG	5 cut(s)	595, 963, 1264, 1412, 1507
DpnI	GATC	7 cut(s)	101, 160, 198, 657, 675, 1688, 1889
DpnII	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
DraIII	CACNNNGTG	2 cut(s)	1599, 1617
DrdI	GACNNNNNNGTC	1 cut(s)	1060
DriI	GACNNNNNGTC	1 cut(s)	286
DseDI	GACNNNNNNGTC	1 cut(s)	1060
EaeI	YGGCCR	2 cut(s)	51, 465
EagI	CGGCCG	1 cut(s)	51
Eam1104I	CTCTTC	3 cut(s)	123, 186, 759
Eam1105I	GACNNNNNGTC	1 cut(s)	286
EarI	CTCTTC	3 cut(s)	123, 186, 759
EciI	GGCGGA	1 cut(s)	740
Ecl136II	GAGCTC	1 cut(s)	109
EclXI	CGGCCG	1 cut(s)	51
Eco130I	CCWWGG	3 cut(s)	10, 633, 898
Eco147I	AGGCCT	1 cut(s)	933
Eco24I	GRGCYC	2 cut(s)	111, 563
Eco47I	GGWCC	4 cut(s)	280, 1699, 1786, 1931
Eco52I	CGGCCG	1 cut(s)	51
Eco53kI	GAGCTC	1 cut(s)	109
Eco57I	CTGAAG	5 cut(s)	104, 210, 783, 1749, 1758
Eco88I	CYCGRG	2 cut(s)	1290, 1693
Eco91I	GGTNACC	1 cut(s)	1643
EcoICRI	GAGCTC	1 cut(s)	109
EcoNI	CCTNNNNNAGG	1 cut(s)	1666
EcoO109I	RGGNCCY	2 cut(s)	280, 1699
EcoO65I	GGTNACC	1 cut(s)	1643
EcoRI	GAATTC	1 cut(s)	628
EcoRII	CCWGG	3 cut(s)	1368, 1667, 1799
EcoT14I	CCWWGG	3 cut(s)	10, 633, 898
EcoT22I	ATGCAT	3 cut(s)	738, 1384, 1542
EcoT38I	GRGCYC	2 cut(s)	111, 563
ErhI	CCWWGG	3 cut(s)	10, 633, 898
Esp3I	CGTCTC	1 cut(s)	248
FalI	AAGNNNNNCTT	4 cut(s)	1400, 1432, 1847, 1879
FaqI	GGGAC	4 cut(s)	18, 293, 1225, 1712
FauI	CCCGC	1 cut(s)	1921
FbaI	TGATCA	1 cut(s)	655
FblI	GTMKAC	2 cut(s)	864, 1064
Fnu4HI	GCNGC	7 cut(s)	209, 212, 1012, 1296, 1524, 1557, 1926
FokI	GGATG	5 cut(s)	734, 881, 1335, 1349, 1527
FriOI	GRGCYC	2 cut(s)	111, 563
Fsp4HI	GCNGC	7 cut(s)	209, 212, 1012, 1296, 1524, 1557, 1926
FspBI	CTAG	1 cut(s)	879
GluI	GCNGC	7 cut(s)	209, 212, 1012, 1296, 1524, 1557, 1926
GsaI	CCCAGC	1 cut(s)	37
GsuI	CTGGAG	5 cut(s)	200, 771, 791, 936, 1129
HaeIII	GGCC	6 cut(s)	53, 92, 262, 467, 933, 1344
HapII	CCGG	5 cut(s)	83, 156, 810, 1291, 1911
HgaI	GACGC	6 cut(s)	15, 228, 316, 337, 341, 956
Hin1I	GRCGYC	4 cut(s)	7, 239, 348, 848
HincII	GTYRAC	1 cut(s)	1609
HindII	GTYRAC	1 cut(s)	1609
HinfI	GANTC	4 cut(s)	687, 1057, 1747, 1879
HpaI	GTTAAC	1 cut(s)	1609
HpaII	CCGG	5 cut(s)	83, 156, 810, 1291, 1911
HphI	GGTGA	5 cut(s)	97, 145, 283, 555, 830
Hpy166II	GTNNAC	6 cut(s)	372, 601, 865, 1065, 1609, 1769
Hpy188III	TCNNGA	9 cut(s)	677, 761, 788, 1210, 1250, 1742, 1784, 1855, 1876
Hpy8I	GTNNAC	6 cut(s)	372, 601, 865, 1065, 1609, 1769
Hpy99I	CGWCG	4 cut(s)	71, 107, 310, 335
HpyAV	CCTTC	8 cut(s)	82, 259, 268, 295, 1130, 1623, 1712, 1782
HpyCH4III	ACNGT	4 cut(s)	830, 1216, 1335, 1649
HpyCH4IV	ACGT	1 cut(s)	848
HpyF10VI	GCNNNNNNNGC	7 cut(s)	236, 458, 1041, 1379, 1413, 1463, 1565
HpyF3I	CTNAG	5 cut(s)	595, 963, 1264, 1412, 1507
HpySE526I	ACGT	1 cut(s)	848
Hsp92I	GRCGYC	4 cut(s)	7, 239, 348, 848
KflI	GGGWCCC	1 cut(s)	280
Ksp22I	TGATCA	1 cut(s)	655
KspAI	GTTAAC	1 cut(s)	1609
Kzo9I	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
LguI	GCTCTTC	1 cut(s)	759
LmnI	GCTCC	5 cut(s)	114, 219, 1110, 1489, 1589
Lsp1109I	GCAGC	6 cut(s)	220, 223, 1307, 1535, 1568, 1937
LweI	GCATC	7 cut(s)	461, 756, 1369, 1527, 1549, 1566, 1672
MabI	ACCWGGT	1 cut(s)	1799
MaeI	CTAG	1 cut(s)	879
MaeII	ACGT	1 cut(s)	848
MaeIII	GTNAC	5 cut(s)	240, 824, 1276, 1448, 1643
MalI	GATC	7 cut(s)	101, 160, 198, 657, 675, 1688, 1889
MboI	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
MflI	RGATCY	1 cut(s)	673
MhlI	GDGCHC	4 cut(s)	111, 364, 563, 1104
MlsI	TGGCCA	1 cut(s)	467
MluCI	AATT	7 cut(s)	628, 1086, 1205, 1655, 1779, 1917, 1936
MluNI	TGGCCA	1 cut(s)	467
MlyI	GAGTC	1 cut(s)	1051
MmeI	TCCRAC	1 cut(s)	1568
Mox20I	TGGCCA	1 cut(s)	467
Mph1103I	ATGCAT	3 cut(s)	738, 1384, 1542
MroXI	GAANNNNTTC	1 cut(s)	1862
MscI	TGGCCA	1 cut(s)	467
MseI	TTAA	3 cut(s)	666, 1485, 1608
MslI	CAYNNNNRTG	2 cut(s)	575, 1842
Msp20I	TGGCCA	1 cut(s)	467
MspA1I	CMGCKG	2 cut(s)	1446, 1928
MspI	CCGG	5 cut(s)	83, 156, 810, 1291, 1911
MspR9I	CCNGG	7 cut(s)	811, 1291, 1292, 1370, 1669, 1801, 1912
Mva1269I	GAATGC	2 cut(s)	1327, 1714
MvaI	CCWGG	3 cut(s)	1370, 1669, 1801
MwoI	GCNNNNNNNGC	7 cut(s)	236, 458, 1041, 1379, 1413, 1463, 1565
NciI	CCSGG	4 cut(s)	811, 1291, 1292, 1912
NcoI	CCATGG	1 cut(s)	10
NdeII	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
NmeAIII	GCCGAG	2 cut(s)	29, 79
NmuCI	GTSAC	1 cut(s)	240
NsiI	ATGCAT	3 cut(s)	738, 1384, 1542
NspI	RCATGY	4 cut(s)	314, 745, 1377, 1515
PagI	TCATGA	1 cut(s)	1875
PceI	AGGCCT	1 cut(s)	933
PciI	ACATGT	1 cut(s)	741
PciSI	GCTCTTC	1 cut(s)	759
PctI	GAATGC	2 cut(s)	1327, 1714
PdmI	GAANNNNTTC	1 cut(s)	1862
PfeI	GAWTC	3 cut(s)	687, 1747, 1879
PflFI	GACNNNGTC	1 cut(s)	1060
PflMI	CCANNNNNTGG	1 cut(s)	1599
PkrI	GCNGC	7 cut(s)	210, 213, 1013, 1297, 1525, 1558, 1927
Ple19I	CGATCG	1 cut(s)	102
PleI	GAGTC	1 cut(s)	1051
PpsI	GAGTC	1 cut(s)	1051
PpuMI	RGGWCCY	2 cut(s)	280, 1699
PscI	ACATGT	1 cut(s)	741
PsiI	TTATAA	1 cut(s)	446
Psp124BI	GAGCTC	1 cut(s)	111
Psp5II	RGGWCCY	2 cut(s)	280, 1699
Psp6I	CCWGG	3 cut(s)	1368, 1667, 1799
PspEI	GGTNACC	1 cut(s)	1643
PspFI	CCCAGC	1 cut(s)	33
PspGI	CCWGG	3 cut(s)	1368, 1667, 1799
PspPI	GGNCC	6 cut(s)	260, 280, 1343, 1699, 1786, 1931
PspPPI	RGGWCCY	2 cut(s)	280, 1699
PstI	CTGCAG	1 cut(s)	1627
PsuI	RGATCY	1 cut(s)	673
PsyI	GACNNNGTC	1 cut(s)	1060
PvuI	CGATCG	1 cut(s)	102
RsaI	GTAC	3 cut(s)	784, 1433, 1770
RsaNI	GTAC	3 cut(s)	783, 1432, 1769
RseI	CAYNNNNRTG	2 cut(s)	575, 1842
SacI	GAGCTC	1 cut(s)	111
SapI	GCTCTTC	1 cut(s)	759
SaqAI	TTAA	3 cut(s)	666, 1485, 1608
SatI	GCNGC	7 cut(s)	209, 212, 1012, 1296, 1524, 1557, 1926
Sau3AI	GATC	7 cut(s)	99, 158, 196, 655, 673, 1686, 1887
Sau96I	GGNCC	6 cut(s)	260, 280, 1343, 1699, 1786, 1931
ScaI	AGTACT	1 cut(s)	784
SchI	GAGTC	1 cut(s)	1051
ScrFI	CCNGG	7 cut(s)	811, 1291, 1292, 1370, 1669, 1801, 1912
SduI	GDGCHC	4 cut(s)	111, 364, 563, 1104
SexAI	ACCWGGT	1 cut(s)	1799
SfaNI	GCATC	7 cut(s)	461, 756, 1369, 1527, 1549, 1566, 1672
SfcI	CTRYAG	4 cut(s)	615, 1065, 1623, 1752
SgrAI	CRCCGGYG	1 cut(s)	82
SinI	GGWCC	4 cut(s)	280, 1699, 1786, 1931
SmaI	CCCGGG	1 cut(s)	1292
SmiMI	CAYNNNNRTG	2 cut(s)	575, 1842
SmlI	CTYRAG	2 cut(s)	365, 1742
SmoI	CTYRAG	2 cut(s)	365, 1742
Sse9I	AATT	7 cut(s)	628, 1086, 1205, 1655, 1779, 1917, 1936
SseBI	AGGCCT	1 cut(s)	933
SsiI	CCGC	4 cut(s)	751, 1011, 1446, 1928
SspMI	CTAG	1 cut(s)	879
SstI	GAGCTC	1 cut(s)	111
StuI	AGGCCT	1 cut(s)	933
StyD4I	CCNGG	7 cut(s)	809, 1289, 1290, 1368, 1667, 1799, 1910
StyI	CCWWGG	3 cut(s)	10, 633, 898
TaaI	ACNGT	4 cut(s)	830, 1216, 1335, 1649
TaiI	ACGT	1 cut(s)	851
TaqI	TCGA	4 cut(s)	102, 1249, 1680, 1866
TaqII	GACCGA	1 cut(s)	1727
TasI	AATT	7 cut(s)	628, 1086, 1205, 1655, 1779, 1917, 1936
TatI	WGTACW	1 cut(s)	782
TauI	GCSGC	1 cut(s)	1014
TfiI	GAWTC	3 cut(s)	687, 1747, 1879
Tru1I	TTAA	3 cut(s)	666, 1485, 1608
Tru9I	TTAA	3 cut(s)	666, 1485, 1608
TscAI	CASTG	8 cut(s)	232, 249, 358, 946, 1051, 1109, 1407, 1459
TseFI	GTSAC	1 cut(s)	240
TseI	GCWGC	6 cut(s)	208, 211, 1295, 1523, 1556, 1925
Tsp45I	GTSAC	1 cut(s)	240
TspDTI	ATGAA	6 cut(s)	540, 938, 1429, 1826, 1864, 1892
TspGWI	ACGGA	1 cut(s)	1602
TspMI	CCCGGG	1 cut(s)	1290
TspRI	CASTG	8 cut(s)	232, 249, 358, 946, 1051, 1109, 1407, 1459
Tth111I	GACNNNGTC	1 cut(s)	1060
Van91I	CCANNNNNTGG	1 cut(s)	1599
VpaK11BI	GGWCC	4 cut(s)	280, 1699, 1786, 1931
XagI	CCTNNNNNAGG	1 cut(s)	1666
XapI	RAATTY	5 cut(s)	628, 1205, 1779, 1917, 1936
XceI	RCATGY	4 cut(s)	314, 745, 1377, 1515
XcmI	CCANNNNNNNNNTGG	2 cut(s)	574, 1797
XmaI	CCCGGG	1 cut(s)	1290
XmiI	GTMKAC	2 cut(s)	864, 1064
XmnI	GAANNNNTTC	1 cut(s)	1862
XspI	CTAG	1 cut(s)	879
ZraI	GACGTC	1 cut(s)	849
ZrmI	AGTACT	1 cut(s)	784
Zsp2I	ATGCAT	3 cut(s)	738, 1384, 1542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.