Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000550

Physical Location & Seq

Genomic Coordinates Forward (+)

50611609 .. 50617501

Gene Structure 5893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1405400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1338 bp

ATGGCGTCAAACGGGAACGGAGAGTTCAGCTTTGCGAGCGGAAACGGAAAGGTGTCAACGGCGGCAAACGGTAACGGAACGGTGAGGAACGGGCTGGCGAAGATCCAGACGCAGAAGAAGGACAATGGGGTGTGTCACGACGACAGCACGCCACCTGTGAAGGCGCAGACGATCGACGAGCTCCACTCGCTGCAGAAGAAGAAGGGCTCACCTAACATTGAAATGGACGAGCAAACGTTCATGGTCCACACAGAAAGAGCTGTGGATTACTTGAATTCTTTGGACAAGGTCTTTGTAAATGATCAATTTTTGAACTGGGGTCTGGAAAACAGATCAAAGTTCGGGTCGTGTCTGCCAGGGCTTATCACTCATTGCTCATGCACAACATGTGAAAACATGTGTGATATTAGCTTAGTGTACGTACAATTCTATAACTCGGTGGGTCGCAGGTGTATCCGACCCACTCTTGAAGAGCTGGAGAATTCTGGTACTCCGGACTTCACTATATACAATGCTGGCCAGTTCTCGTGTAATCGTTGCACCCACTACATGACATCCTCTACTAGCATAGACCTTAATCTTGCTAGGAGGGAAATGGTCATCCTCGGCACCATGTACACTGGGGAAATGAAGAAAGGCCTTTTCAGTGTCATGCATTATCTCATGCCTAAGCGACAAATCCTATCCCTACATTCTGGCTGCAATATGGGGAAAGATGGAGATGTTGCCCTTTTCTTTGGATTGTCAGTGTTGGAAAACATCGTGTTTGATGAGCACACACGAGAGGTGGACTATTCTGACAAATCTGTTACAGAGAACACTCGTGCAGCTTACCCCATCGAATACATTCCCAACGCAAAGATACCATGCGTTGGTCCACATCCAAAGAATGTCATAATTTTGGCATGCGATGCATTTGGTGTGCTCCCTCCAGTGAGCAAGTTAAACCTTGCTCAGACCATGTACCACTTCATCAGTGGCTACACTGCTCTGGTTACCGGAACTGAAGATGGTATCAAGGAGCCAACAGCAACATTATCAGCGTGCTTCGCAATGTTGACTGAGAAGACGCAGAAGCATGGGGCAAGTACTGGACTGTGGTCGGACAAGAATGCATACAATGAGACACCGATGAAGTTGGCTGGTCTATTCAAGAACAACTTTGAGACCTTCACTAACTATCAGATTGGTGAGGAAACCAAGCTAACACAAGTGGATACTAGCAGCAGCCAGTCACAATTTCTAACCGCCGGCAAAACTGTAAAATATCACCTAATTTTGTCACAAGGGTTTTGGGATGGTCAATGGATTGTAAATTACACTAACCTTGTAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004611 GO:0004612 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006094 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0008150 GO:0008152 GO:0009058 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0016020 GO:0016036 GO:0016051 GO:0016829 GO:0016830 GO:0016831 GO:0019318 GO:0019319 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0033554 GO:0042221 GO:0042594 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046364 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070013 GO:0071496 GO:0071704 GO:0098542 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PEPCK_ATP

Protein Analysis

446

Amino Acids

49.31

Weight (kDa)

6.07

Isoelectric Point (pI)

33.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPCK_ATP	PF01293	69 - 249	9.8e-36	Phosphoenolpyruvate carboxykinase
PEPCK_ATP	PF01293	249 - 351	2.5e-47	Phosphoenolpyruvate carboxykinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37870 AT5G65690 AT5G65690 AT5G65690 AT5G65690
fragaria_vesca	FvH4_1g18831 FvH4_1g18850 FvH4_1g18850 FvH4_1g24570 FvH4_4g10540 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_7g04810
malus_domestica	MD01G1046200.v1.1 MD08G1207000.v1.1 MD15G1391200.v1.1 MD15G1405400.v1.1
prunus_persica	Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.6G210900_v2.0.a1 Prupe.6G211000_v2.0.a1
pyrus_communis	pycom01g07230 pycom08g17910 pycom15g26890 pycom16g22360
rosa_chinensis	RchiOBHm_Chr2g0109711 RchiOBHm_Chr2g0109721 RchiOBHm_Chr5g0054131 RchiOBHm_Chr5g0054141 RchiOBHm_Chr5g0054151 RchiOBHm_Chr7g0233021
rosa_laevigata	RLG00000001305 RLG00000017759 RLG00000017760 RLG00000030254 RLG00000030261 RLG00000030264
rosa_multiflora	Rmu_sc0000507.1_g000005 Rmu_sc0000509.1_g000040 Rmu_sc0002076.1_g000010 Rmu_sc0006889.1_g000016 Rmu_sc0006889.1_g000018 Rmu_ssc0000018.1_g000034
rosa_roxburghii	Rroxscaffold_2G00134000 Rroxscaffold_3G00227740 Rroxscaffold_4G00326310 Rroxscaffold_4G00326340
rosa_rugosa	Rorug02G0162100 Rorug02G0162200 Rorug02G0162300 Rorug07G0277900.1
rosa_samantha	Rh2AG214600 Rh2AG214700 Rh2BG224900 Rh2BG225000 Rh2BG225100 Rh2CG216800 Rh2CG216900 Rh2DG220100 Rh2DG220200 Rh5AG354400 Rh5AG354500 Rh5DG381200 Rh5DG381300 Rh7BG405700 Rh7CG452300 Rh7DG421600
rosa_wichuraiana	Rw0G009650 Rw0G009660 Rw1G004940 Rw1G004970 Rw2G016580 Rw5G033400 Rw7G035700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	438
Acc36I	ACCTGC	1 cut(s)	438
AccB1I	GGYRCC	1 cut(s)	608
AccB7I	CCANNNNNTGG	1 cut(s)	872
AccBSI	CCGCTC	1 cut(s)	39
AccIII	TCCGGA	1 cut(s)	493
AciI	CCGC	3 cut(s)	39, 62, 1248
AclI	AACGTT	1 cut(s)	236
AclWI	GGATC	1 cut(s)	97
AcoI	YGGCCR	1 cut(s)	517
AcsI	RAATTY	2 cut(s)	274, 481
AcuI	CTGAAG	1 cut(s)	1026
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	6 cut(s)	419, 423, 490, 617, 965, 1090
AfiI	CCNNNNNNNGG	1 cut(s)	872
AflIII	ACRYGT	2 cut(s)	386, 396
AgsI	TTSAA	5 cut(s)	221, 274, 313, 470, 1153
AjnI	CCWGG	1 cut(s)	355
AluBI	AGCT	7 cut(s)	30, 181, 260, 411, 475, 830, 1204
AluI	AGCT	7 cut(s)	30, 181, 260, 411, 475, 830, 1204
Alw21I	GWGCWC	3 cut(s)	183, 777, 927
Alw26I	GTCTC	2 cut(s)	1118, 1160
AlwI	GGATC	1 cut(s)	97
Aor13HI	TCCGGA	1 cut(s)	493
AoxI	GGCC	2 cut(s)	517, 637
ApeKI	GCWGC	5 cut(s)	190, 699, 827, 1224, 1227
ApoI	RAATTY	2 cut(s)	274, 481
ArsI	GACNNNNNNTTYG	4 cut(s)	275, 307, 329, 361
Asp700I	GAANNNNTTC	1 cut(s)	846
AspLEI	GCGC	1 cut(s)	166
AspS9I	GGNCC	2 cut(s)	244, 875
AsuHPI	GGTGA	4 cut(s)	94, 201, 1202, 1262
AvaII	GGWCC	2 cut(s)	244, 875
BalI	TGGCCA	1 cut(s)	519
BanI	GGYRCC	1 cut(s)	608
BanII	GRGCYC	2 cut(s)	183, 209
BauI	CACGAG	3 cut(s)	526, 780, 822
BbsI	GAAGAC	1 cut(s)	1073
Bbv12I	GWGCWC	3 cut(s)	183, 777, 927
BbvI	GCAGC	5 cut(s)	177, 686, 839, 1236, 1239
BccI	CCATC	4 cut(s)	710, 845, 1004, 1292
BceAI	ACGGC	1 cut(s)	75
BciT130I	CCWGG	1 cut(s)	357
BciVI	GTATCC	2 cut(s)	464, 1210
BclI	TGATCA	1 cut(s)	301
BcoDI	GTCTC	2 cut(s)	1118, 1160
BfaI	CTAG	3 cut(s)	564, 585, 1221
BfmI	CTRYAG	1 cut(s)	191
BfuAI	ACCTGC	1 cut(s)	438
BfuI	GTATCC	2 cut(s)	464, 1210
BisI	GCNGC	6 cut(s)	63, 191, 700, 828, 1225, 1228
BlsI	GCNGC	6 cut(s)	64, 192, 701, 829, 1226, 1229
BmcAI	AGTACT	1 cut(s)	1090
Bme1390I	CCNGG	1 cut(s)	357
Bme18I	GGWCC	2 cut(s)	244, 875
BmgT120I	GGNCC	2 cut(s)	244, 875
BmiI	GGNNCC	2 cut(s)	610, 1023
BmrFI	CCNGG	1 cut(s)	357
BmrI	ACTGGG	2 cut(s)	325, 630
BmsI	GCATC	1 cut(s)	901
BmuI	ACTGGG	2 cut(s)	325, 630
BoxI	GACNNNNGTC	1 cut(s)	1099
BpiI	GAAGAC	1 cut(s)	1073
BplI	GAGNNNNNCTC	2 cut(s)	170, 202
BpmI	CTGGAG	2 cut(s)	497, 915
Bpu10I	CCTNAGC	1 cut(s)	669
BsaAI	YACGTR	1 cut(s)	421
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	1160
BsaJI	CCNNGG	2 cut(s)	356, 604
BsaWI	WCCGGW	2 cut(s)	493, 998
Bsc4I	CCNNNNNNNGG	1 cut(s)	872
Bse118I	RCCGGY	1 cut(s)	1250
Bse1I	ACTGG	6 cut(s)	320, 520, 625, 932, 1096, 1231
Bse3DI	GCAATG	2 cut(s)	370, 1059
BseAI	TCCGGA	1 cut(s)	493
BseBI	CCWGG	1 cut(s)	357
BseDI	CCNNGG	2 cut(s)	356, 604
BseGI	GGATG	4 cut(s)	554, 600, 880, 1303
BseLI	CCNNNNNNNGG	1 cut(s)	872
BseMI	GCAATG	2 cut(s)	370, 1059
BseMII	CTCAG	2 cut(s)	968, 1053
BseNI	ACTGG	6 cut(s)	320, 520, 625, 932, 1096, 1231
BseXI	GCAGC	5 cut(s)	177, 686, 839, 1236, 1239
BsgI	GTGCAG	1 cut(s)	846
Bsh1285I	CGRYCG	1 cut(s)	174
BshFI	GGCC	2 cut(s)	519, 639
BshNI	GGYRCC	1 cut(s)	608
BsiEI	CGRYCG	1 cut(s)	174
BsiHKAI	GWGCWC	3 cut(s)	183, 777, 927
BsiSI	CCGG	3 cut(s)	494, 999, 1251
BslI	CCNNNNNNNGG	1 cut(s)	872
BsmAI	GTCTC	2 cut(s)	1118, 1160
BsmI	GAATGC	1 cut(s)	1117
BsnI	GGCC	2 cut(s)	519, 639
Bso31I	GGTCTC	1 cut(s)	1160
Bsp1286I	GDGCHC	4 cut(s)	183, 209, 777, 927
Bsp13I	TCCGGA	1 cut(s)	493
Bsp1407I	TGTACA	1 cut(s)	615
Bsp143I	GATC	4 cut(s)	102, 171, 301, 332
BspACI	CCGC	3 cut(s)	39, 62, 1248
BspANI	GGCC	2 cut(s)	519, 639
BspCNI	CTCAG	2 cut(s)	967, 1054
BspEI	TCCGGA	1 cut(s)	493
BspLI	GGNNCC	2 cut(s)	610, 1023
BspMAI	CTGCAG	1 cut(s)	195
BspMI	ACCTGC	1 cut(s)	438
BspPI	GGATC	1 cut(s)	97
BspQI	GCTCTTC	1 cut(s)	465
BspT107I	GGYRCC	1 cut(s)	608
BspTNI	GGTCTC	1 cut(s)	1160
BsrBI	CCGCTC	1 cut(s)	39
BsrDI	GCAATG	2 cut(s)	370, 1059
BsrFI	RCCGGY	1 cut(s)	1250
BsrGI	TGTACA	1 cut(s)	615
BsrI	ACTGG	6 cut(s)	320, 520, 625, 932, 1096, 1231
BssAI	RCCGGY	1 cut(s)	1250
BssECI	CCNNGG	2 cut(s)	356, 604
BssMI	GATC	4 cut(s)	102, 171, 301, 332
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	3 cut(s)	526, 780, 822
Bst2BI	CACGAG	3 cut(s)	526, 780, 822
Bst2UI	CCWGG	1 cut(s)	357
Bst4CI	ACNGT	4 cut(s)	71, 82, 1098, 1261
Bst6I	CTCTTC	1 cut(s)	465
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	1 cut(s)	911
BstAUI	TGTACA	1 cut(s)	615
BstBAI	YACGTR	1 cut(s)	421
BstC8I	GCNNGC	7 cut(s)	37, 96, 149, 517, 907, 1045, 1252
BstDEI	CTNAG	4 cut(s)	412, 669, 954, 1062
BstEII	GGTNACC	1 cut(s)	994
BstF5I	GGATG	4 cut(s)	554, 600, 880, 1303
BstHHI	GCGC	1 cut(s)	166
BstKTI	GATC	4 cut(s)	105, 174, 304, 335
BstMAI	GTCTC	2 cut(s)	1118, 1160
BstMBI	GATC	4 cut(s)	102, 171, 301, 332
BstMCI	CGRYCG	1 cut(s)	174
BstMWI	GCNNNNNNNGC	4 cut(s)	36, 187, 911, 1049
BstNI	CCWGG	1 cut(s)	357
BstNSI	RCATGY	3 cut(s)	390, 400, 909
BstPAI	GACNNNNGTC	1 cut(s)	1099
BstPI	GGTNACC	1 cut(s)	994
BstSCI	CCNGG	1 cut(s)	355
BstSFI	CTRYAG	1 cut(s)	191
BstSNI	TACGTA	1 cut(s)	421
BstV1I	GCAGC	5 cut(s)	177, 686, 839, 1236, 1239
BstV2I	GAAGAC	1 cut(s)	1073
BstX2I	RGATCY	1 cut(s)	102
BstYI	RGATCY	1 cut(s)	102
BsuI	GTATCC	2 cut(s)	464, 1210
BsuRI	GGCC	2 cut(s)	519, 639
BtgZI	GCGATG	1 cut(s)	924
BtsCI	GGATG	4 cut(s)	554, 600, 880, 1303
BtsI	GCAGTG	1 cut(s)	984
BtsIMutI	CAGTG	6 cut(s)	618, 652, 753, 939, 982, 984
BveI	ACCTGC	1 cut(s)	438
Cac8I	GCNNGC	7 cut(s)	37, 96, 149, 517, 907, 1045, 1252
CfoI	GCGC	1 cut(s)	166
Cfr10I	RCCGGY	1 cut(s)	1250
Cfr13I	GGNCC	2 cut(s)	244, 875
CseI	GACGC	2 cut(s)	118, 1078
Csp6I	GTAC	6 cut(s)	418, 422, 489, 616, 964, 1089
CviQI	GTAC	6 cut(s)	418, 422, 489, 616, 964, 1089
DdeI	CTNAG	4 cut(s)	412, 669, 954, 1062
DpnI	GATC	4 cut(s)	104, 173, 303, 334
DpnII	GATC	4 cut(s)	102, 171, 301, 332
EaeI	YGGCCR	1 cut(s)	517
Eam1104I	CTCTTC	1 cut(s)	465
EarI	CTCTTC	1 cut(s)	465
Ecl136II	GAGCTC	1 cut(s)	181
Eco105I	TACGTA	1 cut(s)	421
Eco147I	AGGCCT	1 cut(s)	639
Eco24I	GRGCYC	2 cut(s)	183, 209
Eco31I	GGTCTC	1 cut(s)	1160
Eco47I	GGWCC	2 cut(s)	244, 875
Eco53kI	GAGCTC	1 cut(s)	181
Eco57I	CTGAAG	1 cut(s)	1026
Eco91I	GGTNACC	1 cut(s)	994
EcoICRI	GAGCTC	1 cut(s)	181
EcoO65I	GGTNACC	1 cut(s)	994
EcoRI	GAATTC	2 cut(s)	274, 481
EcoRII	CCWGG	1 cut(s)	355
EcoT22I	ATGCAT	3 cut(s)	657, 916, 1117
EcoT38I	GRGCYC	2 cut(s)	183, 209
FalI	AAGNNNNNCTT	2 cut(s)	1145, 1177
FbaI	TGATCA	1 cut(s)	301
Fnu4HI	GCNGC	6 cut(s)	63, 191, 700, 828, 1225, 1228
FokI	GGATG	4 cut(s)	541, 587, 867, 1310
FriOI	GRGCYC	2 cut(s)	183, 209
Fsp4HI	GCNGC	6 cut(s)	63, 191, 700, 828, 1225, 1228
FspBI	CTAG	3 cut(s)	564, 585, 1221
GlaI	GCGC	1 cut(s)	165
GluI	GCNGC	6 cut(s)	63, 191, 700, 828, 1225, 1228
GsuI	CTGGAG	2 cut(s)	497, 915
HaeIII	GGCC	2 cut(s)	519, 639
HapII	CCGG	3 cut(s)	494, 999, 1251
HgaI	GACGC	2 cut(s)	118, 1078
HhaI	GCGC	1 cut(s)	166
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	1 cut(s)	164
HinP1I	GCGC	1 cut(s)	164
HincII	GTYRAC	2 cut(s)	57, 1059
HindII	GTYRAC	2 cut(s)	57, 1059
HpaII	CCGG	3 cut(s)	494, 999, 1251
HphI	GGTGA	4 cut(s)	94, 201, 1202, 1262
Hpy166II	GTNNAC	8 cut(s)	57, 247, 418, 618, 790, 878, 1059, 1333
Hpy188I	TCNGA	5 cut(s)	458, 799, 957, 1105, 1185
Hpy188III	TCNNGA	6 cut(s)	106, 137, 323, 467, 494, 1153
Hpy8I	GTNNAC	8 cut(s)	57, 247, 418, 618, 790, 878, 1059, 1333
Hpy99I	CGWCG	2 cut(s)	143, 179
HpyAV	CCTTC	4 cut(s)	112, 154, 196, 1180
HpyCH4III	ACNGT	4 cut(s)	71, 82, 1098, 1261
HpyCH4IV	ACGT	2 cut(s)	236, 420
HpyCH4V	TGCA	8 cut(s)	193, 381, 540, 655, 702, 827, 914, 1115
HpyF10VI	GCNNNNNNNGC	4 cut(s)	36, 187, 911, 1049
HpyF3I	CTNAG	4 cut(s)	412, 669, 954, 1062
HpySE526I	ACGT	2 cut(s)	236, 420
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	1 cut(s)	164
Kpn2I	TCCGGA	1 cut(s)	493
KroI	GCCGGC	1 cut(s)	1250
KroNI	GCCGGC	1 cut(s)	1252
Ksp22I	TGATCA	1 cut(s)	301
Kzo9I	GATC	4 cut(s)	102, 171, 301, 332
LguI	GCTCTTC	1 cut(s)	465
LmnI	GCTCC	3 cut(s)	186, 930, 1021
Lsp1109I	GCAGC	5 cut(s)	177, 686, 839, 1236, 1239
LweI	GCATC	1 cut(s)	901
MaeI	CTAG	3 cut(s)	564, 585, 1221
MaeII	ACGT	2 cut(s)	236, 420
MaeIII	GTNAC	6 cut(s)	71, 134, 808, 994, 1233, 1281
MalI	GATC	4 cut(s)	104, 173, 303, 334
MbiI	CCGCTC	1 cut(s)	39
MboI	GATC	4 cut(s)	102, 171, 301, 332
MboII	GAAGA	8 cut(s)	112, 127, 208, 211, 482, 643, 1019, 1078
MflI	RGATCY	1 cut(s)	102
MhlI	GDGCHC	4 cut(s)	183, 209, 777, 927
MlsI	TGGCCA	1 cut(s)	519
MluCI	AATT	8 cut(s)	274, 305, 425, 481, 897, 1238, 1275, 1315
MluNI	TGGCCA	1 cut(s)	519
MmeI	TCCRAC	3 cut(s)	481, 732, 1083
MnlI	CCTC	7 cut(s)	78, 568, 582, 614, 778, 939, 1186
Mox20I	TGGCCA	1 cut(s)	519
Mph1103I	ATGCAT	3 cut(s)	657, 916, 1117
MroI	TCCGGA	1 cut(s)	493
MroNI	GCCGGC	1 cut(s)	1250
MroXI	GAANNNNTTC	1 cut(s)	846
MscI	TGGCCA	1 cut(s)	519
MseI	TTAA	2 cut(s)	576, 944
MslI	CAYNNNNRTG	1 cut(s)	221
Msp20I	TGGCCA	1 cut(s)	519
MspI	CCGG	3 cut(s)	494, 999, 1251
MspR9I	CCNGG	1 cut(s)	357
Mva1269I	GAATGC	1 cut(s)	1117
MvaI	CCWGG	1 cut(s)	357
MwoI	GCNNNNNNNGC	4 cut(s)	36, 187, 911, 1049
NaeI	GCCGGC	1 cut(s)	1252
NdeII	GATC	4 cut(s)	102, 171, 301, 332
NgoMIV	GCCGGC	1 cut(s)	1250
NlaIV	GGNNCC	2 cut(s)	610, 1023
NmeAIII	GCCGAG	1 cut(s)	585
NmuCI	GTSAC	3 cut(s)	134, 1233, 1281
NsiI	ATGCAT	3 cut(s)	657, 916, 1117
NspI	RCATGY	3 cut(s)	390, 400, 909
PaeI	GCATGC	1 cut(s)	909
PaqCI	CACCTGC	1 cut(s)	438
PceI	AGGCCT	1 cut(s)	639
PciI	ACATGT	2 cut(s)	386, 396
PciSI	GCTCTTC	1 cut(s)	465
PctI	GAATGC	1 cut(s)	1117
PdiI	GCCGGC	1 cut(s)	1252
PdmI	GAANNNNTTC	1 cut(s)	846
PflFI	GACNNNGTC	1 cut(s)	287
PflMI	CCANNNNNTGG	1 cut(s)	872
PkrI	GCNGC	6 cut(s)	64, 192, 701, 829, 1226, 1229
Ple19I	CGATCG	1 cut(s)	174
Ppu21I	YACGTR	1 cut(s)	421
PscI	ACATGT	2 cut(s)	386, 396
PshAI	GACNNNNGTC	1 cut(s)	1099
Psp124BI	GAGCTC	1 cut(s)	183
Psp1406I	AACGTT	1 cut(s)	236
Psp6I	CCWGG	1 cut(s)	355
PspEI	GGTNACC	1 cut(s)	994
PspGI	CCWGG	1 cut(s)	355
PspN4I	GGNNCC	2 cut(s)	610, 1023
PspPI	GGNCC	2 cut(s)	244, 875
PstI	CTGCAG	1 cut(s)	195
PsuI	RGATCY	1 cut(s)	102
PsyI	GACNNNGTC	1 cut(s)	287
PvuI	CGATCG	1 cut(s)	174
RsaI	GTAC	6 cut(s)	419, 423, 490, 617, 965, 1090
RsaNI	GTAC	6 cut(s)	418, 422, 489, 616, 964, 1089
RseI	CAYNNNNRTG	1 cut(s)	221
SacI	GAGCTC	1 cut(s)	183
SapI	GCTCTTC	1 cut(s)	465
SaqAI	TTAA	2 cut(s)	576, 944
SatI	GCNGC	6 cut(s)	63, 191, 700, 828, 1225, 1228
Sau3AI	GATC	4 cut(s)	102, 171, 301, 332
Sau96I	GGNCC	2 cut(s)	244, 875
ScaI	AGTACT	1 cut(s)	1090
ScrFI	CCNGG	1 cut(s)	357
SduI	GDGCHC	4 cut(s)	183, 209, 777, 927
SfaNI	GCATC	1 cut(s)	901
SfcI	CTRYAG	1 cut(s)	191
SinI	GGWCC	2 cut(s)	244, 875
SmiMI	CAYNNNNRTG	1 cut(s)	221
SnaBI	TACGTA	1 cut(s)	421
SphI	GCATGC	1 cut(s)	909
Sse9I	AATT	8 cut(s)	274, 305, 425, 481, 897, 1238, 1275, 1315
SseBI	AGGCCT	1 cut(s)	639
SsiI	CCGC	3 cut(s)	39, 62, 1248
SspMI	CTAG	3 cut(s)	564, 585, 1221
SstI	GAGCTC	1 cut(s)	183
StuI	AGGCCT	1 cut(s)	639
StyD4I	CCNGG	1 cut(s)	355
TaaI	ACNGT	4 cut(s)	71, 82, 1098, 1261
TaiI	ACGT	2 cut(s)	239, 423
TaqI	TCGA	2 cut(s)	174, 840
TasI	AATT	8 cut(s)	274, 305, 425, 481, 897, 1238, 1275, 1315
TatI	WGTACW	2 cut(s)	615, 1088
TauI	GCSGC	1 cut(s)	65
Tru1I	TTAA	2 cut(s)	576, 944
Tru9I	TTAA	2 cut(s)	576, 944
TscAI	CASTG	6 cut(s)	625, 652, 753, 939, 982, 991
TseFI	GTSAC	3 cut(s)	134, 1233, 1281
TseI	GCWGC	5 cut(s)	190, 699, 827, 1224, 1227
Tsp45I	GTSAC	3 cut(s)	134, 1233, 1281
TspDTI	ATGAA	4 cut(s)	229, 644, 961, 1148
TspGWI	ACGGA	3 cut(s)	33, 60, 90
TspRI	CASTG	6 cut(s)	625, 652, 753, 939, 982, 991
Tth111I	GACNNNGTC	1 cut(s)	287
Van91I	CCANNNNNTGG	1 cut(s)	872
VpaK11BI	GGWCC	2 cut(s)	244, 875
XapI	RAATTY	2 cut(s)	274, 481
XceI	RCATGY	3 cut(s)	390, 400, 909
XcmI	CCANNNNNNNNNTGG	1 cut(s)	974
XmnI	GAANNNNTTC	1 cut(s)	846
XspI	CTAG	3 cut(s)	564, 585, 1221
ZrmI	AGTACT	1 cut(s)	1090
Zsp2I	ATGCAT	3 cut(s)	657, 916, 1117

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.