Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0000550

Physical Location & Seq

Genomic Coordinates Forward (+)

13852181 .. 13859519

Gene Structure 7339 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001305

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGGCTAACAACGCAGGTGAAAATGGAGACTTCAAGTTTGGTCGGATCGGAAGTATGGGCCGTCACGGACTTCCAAAGATCCAAACCCAACATGAGACCAAGGAGGTCTGCCACGACGACACTGCAACTCCGGTCAAAGCCAAGACAGTGGACGAGCTCCACTCTCTTCAGAAGAAGAGGTCATCGCAGCCCAGCACTCCCCACACCGCTACAGGGCAGCAAGGGTCGGCTTTCAACTCCATTTCCGAGGAGGATCGGCAGAAACAGCAGCTCCAGTCCATCAGTGCGTCTTTGGCGTCGCTGACGAGGGAGACGGGGCCGAAGGTGGTGAGAGGTGACCCGGCGAGGAGGACTGATGGGCAGAAAGCCGTGCATGTTGAGCATCATCTGTATGATAGTGCGCTCTTTGGGCTCAGTGATAGTGCTCTCAAGTTCACCCACATCCTCTACAACCTCTCTCCTGCTGAACTGTATGAGCAAGCTATCAAGTATGAGAAAGGTTCATTTATAACAGCATCCGGTGCCTTGGCCACACTGTCTGGAGCAAAGACAGGCCGTTCACCAAGAGACAAACGAGTTGTGAAAGATGAAGAGACCGAAACTGAGCTTTGGTGGGGAAAGGGTTCACCAAACATTGAAATGGATGAACACACTTTCTTAGTGAACAGAGAACGAGCTGTTGATTACTTGAATTCTTTGGACAAGGTGTTTGTGAATGATCAATTTTTGAACTGGGACCCAGAACATCGAATCAAAGTTCGAATTGTATCTGCAAGAGCTTACCACTCCCTCTTTATGCATAACATGTGCATCCGACCCACTCCTGAGGAGCTGGAGGAGTTTGGTACTCCAGACTTCACAATATACAATGCTGGGCAGTTCCCATGTAACCGTTATACCCACTACATGACTTCCTCTACCAGCATAGATCTCAATCTTGCCAGAAGGGAAATGGTCATCCTTGGCACCCAGTATGCTGGTGAAATGAAGAAAGGCCTTTTTAGTGTAATGCACTATCTCATGCCTAAGCGTCGAATCCTCTCCCTACATTCTGGCTGCAATATGGGGAAAGATGGCGATGTTGCCCTTTTCTTTGGCTTATCAGGAACTGGGAAGACAACTCTGTCTACAGATCATAATAGGTATTTAATCGGGGATGATGAGCACTGCTGGAGTGACAATGGTGTGTCAAACATTGAAGGTGGTTGCTATGCTAAGTGCATTGATTTGTCAAAAGACAAGGAACCAGATATTTGGAACGCTATCAAATTTGGGACTGTTTTGGAGAATGTTGTGTTTGATGAGCATAACCGAGAAGTGGATTATTCAGAGAAATGTGTTACAGAGAACACCCGGGCAGCATATCCTATAGAGTACATACCAAATGCTAAAATACCATGTGTTGGTCCTCATCCGAAAAATGTCATTCTCCTGGCCTGTGATGCATTTGGTGTGCTCCCACCTGTGAGCAAGTTGAGTTTGGCCCAAACAATGTACCATTTTATCAGCGGCTACACTGCTCTGGTTGCTGGAACTGAGGATGGTATCAAGGAACCACAAGCTACATTCTCAGCATGCTTCGGTGCAGCATTTATAATGATGCATCCAACAAAGTATGCAGCAATGTTGGCTGAAAAGATGCAAAAACACGGGGCTACAGGATGGCTCGTCAATACAGGCTGGTCAGCAGGACGGTATGGAACAGGTAACCGCATCAAATTAGCTCATACCAGGAAGATTATAGATGCTATTCACTCGGGTACCCTTTTGAATGCAACTTACACCAAGACTGAAGTATTTGGACTTGAGATCCCAACTGAGATTGAGGGTGTACCTTCAGTAATTCTGGATCCAATTAACACTTGGTCAGACAAGAAAGCTTACAAAGACACACTACTTAAGTTGGGTGGTCTGTTCAAGAAGAATTTTGAGTTAGTGAAGCTCAGCAATGTCGTCCAAGCGGAAGGATTAGAGGAAAGAAGCCTCCTCCTGGACAATGTAACCAGGAAGATGACTGACTGCTGCAAAGCTGGAAAAATGTACCCAACATACACATGCTCACCACCAATGTCAGGTGGTGACGGAGGAGGCCCATCAGAATGTGATGGCAAGTACCACAATGACAACACTCCAGTTGTGGCATTATCTACCGGATGGTATAATGGTGGATCACGATGCCTTAACAACATCAGAATTAATGGTAATGGGCGCAGTGTGGTGGCCATGGTGGTGGATGAGTGTGACTCGACTGAGGGATGTGATGCGGGCCATGATTATCAGCCTCCTTGTCCCAACAATATTGTTGATGCATCGAAGGCTGTCTGGAAAGCCTTAGGTGTATCTGAACACAACTGGGGTGGCTTGGTTATCACATGGTCTGACGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004611 GO:0004612 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006094 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0008150 GO:0008152 GO:0009058 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0016020 GO:0016036 GO:0016051 GO:0016829 GO:0016830 GO:0016831 GO:0019318 GO:0019319 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0033554 GO:0042221 GO:0042594 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046364 GO:0046686 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070013 GO:0071496 GO:0071704 GO:0098542 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PEPCK_ATP

Protein Analysis

796

Amino Acids

87.7

Weight (kDa)

6.29

Isoelectric Point (pI)

42.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPCK_ATP	PF01293	147 - 613	3e-212	Phosphoenolpyruvate carboxykinase
KWL1	PF24300	668 - 795	5.7e-40	Kiwellin-1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37870 AT5G65690 AT5G65690 AT5G65690 AT5G65690
fragaria_vesca	FvH4_1g18831 FvH4_1g18850 FvH4_1g18850 FvH4_1g24570 FvH4_4g10540 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_5g33740 FvH4_7g04810
malus_domestica	MD01G1046200.v1.1 MD08G1207000.v1.1 MD15G1391200.v1.1 MD15G1405400.v1.1
prunus_persica	Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.1G541200_v2.0.a1 Prupe.6G210900_v2.0.a1 Prupe.6G211000_v2.0.a1
pyrus_communis	pycom01g07230 pycom08g17910 pycom15g26890 pycom16g22360
rosa_chinensis	RchiOBHm_Chr2g0109711 RchiOBHm_Chr2g0109721 RchiOBHm_Chr5g0054131 RchiOBHm_Chr5g0054141 RchiOBHm_Chr5g0054151 RchiOBHm_Chr7g0233021
rosa_laevigata	RLG00000001305 RLG00000017759 RLG00000017760 RLG00000030254 RLG00000030261 RLG00000030264
rosa_multiflora	Rmu_sc0000507.1_g000005 Rmu_sc0000509.1_g000040 Rmu_sc0002076.1_g000010 Rmu_sc0006889.1_g000016 Rmu_sc0006889.1_g000018 Rmu_ssc0000018.1_g000034
rosa_roxburghii	Rroxscaffold_2G00134000 Rroxscaffold_3G00227740 Rroxscaffold_4G00326310 Rroxscaffold_4G00326340
rosa_rugosa	Rorug02G0162100 Rorug02G0162200 Rorug02G0162300 Rorug07G0277900.1
rosa_samantha	Rh2AG214600 Rh2AG214700 Rh2BG224900 Rh2BG225000 Rh2BG225100 Rh2CG216800 Rh2CG216900 Rh2DG220100 Rh2DG220200 Rh5AG354400 Rh5AG354500 Rh5DG381200 Rh5DG381300 Rh7BG405700 Rh7CG452300 Rh7DG421600
rosa_wichuraiana	Rw0G009650 Rw0G009660 Rw1G004940 Rw1G004970 Rw2G016580 Rw5G033400 Rw7G035700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	509, 1595
AarI	CACCTGC	1 cut(s)	5
AasI	GACNNNNNNGTC	1 cut(s)	1123
Acc36I	ACCTGC	1 cut(s)	5
Acc65I	GGTACC	1 cut(s)	1760
AccB1I	GGYRCC	3 cut(s)	521, 965, 1760
AccB7I	CCANNNNNTGG	1 cut(s)	1403
AccI	GTMKAC	1 cut(s)	1127
AciI	CCGC	5 cut(s)	207, 1509, 1711, 1961, 2264
AclWI	GGATC	7 cut(s)	53, 73, 261, 1804, 1844, 1857, 2176
AcoI	YGGCCR	2 cut(s)	528, 2220
AcsI	RAATTY	3 cut(s)	691, 1268, 1924
AcuI	CTGAAG	3 cut(s)	152, 1812, 1821
AcyI	GRCGYC	1 cut(s)	296
AfaI	GTAC	7 cut(s)	847, 1376, 1496, 1762, 1833, 2042, 2114
AfiI	CCNNNNNNNGG	5 cut(s)	213, 1318, 1403, 1990, 2072
AflII	CTTAAG	1 cut(s)	1898
AflIII	ACRYGT	1 cut(s)	804
AgsI	TTSAA	8 cut(s)	34, 235, 638, 691, 730, 1199, 1771, 1918
AjnI	CCWGG	4 cut(s)	1431, 1730, 1989, 2003
Alw21I	GWGCWC	4 cut(s)	159, 427, 1167, 1458
Alw26I	GTCTC	5 cut(s)	21, 89, 305, 561, 587
AlwI	GGATC	7 cut(s)	53, 73, 261, 1804, 1844, 1857, 2176
AlwNI	CAGNNNCTG	1 cut(s)	1109
Ama87I	CYCGRG	2 cut(s)	1353, 1756
ApeKI	GCWGC	8 cut(s)	187, 217, 268, 1056, 1358, 1586, 1619, 2022
ApoI	RAATTY	3 cut(s)	691, 1268, 1924
ArsI	GACNNNNNNTTYG	4 cut(s)	20, 52, 692, 724
AseI	ATTAAT	1 cut(s)	2196
Asp700I	GAANNNNTTC	1 cut(s)	622
Asp718I	GGTACC	1 cut(s)	1760
AspLEI	GCGC	2 cut(s)	403, 2211
AspS9I	GGNCC	7 cut(s)	58, 317, 736, 1406, 1483, 2090, 2266
AsuC2I	CCSGG	3 cut(s)	341, 1354, 1355
AsuHPI	GGTGA	9 cut(s)	29, 340, 347, 427, 552, 618, 992, 2052, 2090
AsuII	TTCGAA	1 cut(s)	760
AvaI	CYCGRG	2 cut(s)	1353, 1756
AvaII	GGWCC	2 cut(s)	736, 1406
AxyI	CCTNAGG	2 cut(s)	825, 2332
BaeI	ACNNNNGTAYC	2 cut(s)	1744, 1777
BalI	TGGCCA	2 cut(s)	530, 2222
BamHI	GGATCC	1 cut(s)	1849
BanI	GGYRCC	3 cut(s)	521, 965, 1760
BanII	GRGCYC	2 cut(s)	159, 414
BbsI	GAAGAC	1 cut(s)	1121
Bbv12I	GWGCWC	4 cut(s)	159, 427, 1167, 1458
BbvI	GCAGC	8 cut(s)	199, 229, 280, 1043, 1370, 1598, 1631, 2009
BccI	CCATC	8 cut(s)	287, 350, 1067, 1535, 1656, 2099, 2101, 2148
BceAI	ACGGC	3 cut(s)	45, 353, 540
BcgI	CGANNNNNNTGC	2 cut(s)	104, 138
BciT130I	CCWGG	4 cut(s)	1433, 1732, 1991, 2005
BclI	TGATCA	1 cut(s)	718
BcnI	CCSGG	3 cut(s)	341, 1354, 1355
BcoDI	GTCTC	5 cut(s)	21, 89, 305, 561, 587
BfmI	CTRYAG	4 cut(s)	210, 1128, 1368, 1656
BfrI	CTTAAG	1 cut(s)	1898
BfuAI	ACCTGC	1 cut(s)	5
BglII	AGATCT	1 cut(s)	928
BisI	GCNGC	9 cut(s)	188, 218, 269, 1057, 1359, 1510, 1587, 1620, 2023
BlpI	GCTNAGC	1 cut(s)	1943
BlsI	GCNGC	9 cut(s)	189, 219, 270, 1058, 1360, 1511, 1588, 1621, 2024
Bme1390I	CCNGG	7 cut(s)	341, 1354, 1355, 1433, 1732, 1991, 2005
Bme18I	GGWCC	2 cut(s)	736, 1406
BmeT110I	CYCGRG	2 cut(s)	1353, 1756
BmgT120I	GGNCC	7 cut(s)	58, 317, 736, 1406, 1483, 2090, 2266
BmiI	GGNNCC	9 cut(s)	318, 523, 737, 738, 967, 1245, 1554, 1762, 1851
BmrFI	CCNGG	7 cut(s)	341, 1354, 1355, 1433, 1732, 1991, 2005
BmrI	ACTGGG	4 cut(s)	742, 964, 1119, 2362
BmuI	ACTGGG	4 cut(s)	742, 964, 1119, 2362
BpiI	GAAGAC	1 cut(s)	1121
BplI	GAGNNNNNCTC	4 cut(s)	146, 178, 2228, 2260
BpmI	CTGGAG	6 cut(s)	257, 561, 834, 854, 1192, 2115
Bpu10I	CCTNAGC	1 cut(s)	1026
Bpu1102I	GCTNAGC	1 cut(s)	1943
Bpu14I	TTCGAA	1 cut(s)	760
BpuEI	CTTGAG	2 cut(s)	413, 1826
BpuMI	CCSGG	3 cut(s)	341, 1354, 1355
BsaBI	GATNNNNATC	1 cut(s)	933
BsaHI	GRCGYC	1 cut(s)	296
BsaI	GGTCTC	2 cut(s)	89, 587
BsaJI	CCNNGG	6 cut(s)	99, 246, 525, 961, 1353, 2223
BsaWI	WCCGGW	3 cut(s)	130, 518, 2150
BsaXI	ACNNNNNCTCC	4 cut(s)	112, 142, 255, 285
Bsc4I	CCNNNNNNNGG	5 cut(s)	213, 1318, 1403, 1990, 2072
Bse1I	ACTGG	6 cut(s)	274, 737, 970, 1114, 2132, 2357
Bse21I	CCTNAGG	2 cut(s)	825, 2332
Bse3DI	GCAATG	2 cut(s)	1629, 1954
Bse8I	GATNNNNATC	1 cut(s)	933
BseBI	CCWGG	4 cut(s)	1433, 1732, 1991, 2005
BseDI	CCNNGG	6 cut(s)	99, 246, 525, 961, 1353, 2223
BseJI	GATNNNNATC	1 cut(s)	933
BseLI	CCNNNNNNNGG	5 cut(s)	213, 1318, 1403, 1990, 2072
BseMI	GCAATG	2 cut(s)	1629, 1954
BseMII	CTCAG	8 cut(s)	427, 594, 816, 1527, 1584, 1809, 1957, 2241
BseNI	ACTGG	6 cut(s)	274, 737, 970, 1114, 2132, 2357
BseRI	GAGGAG	6 cut(s)	263, 361, 842, 851, 1976, 2100
BseXI	GCAGC	8 cut(s)	199, 229, 280, 1043, 1370, 1598, 1631, 2009
BseYI	CCCAGC	2 cut(s)	191, 872
BsgI	GTGCAG	1 cut(s)	1605
BshNI	GGYRCC	3 cut(s)	521, 965, 1760
BsiHKAI	GWGCWC	4 cut(s)	159, 427, 1167, 1458
BsiHKCI	CYCGRG	2 cut(s)	1353, 1756
BsiSI	CCGG	5 cut(s)	131, 341, 519, 1354, 2151
BslFI	GGGAC	3 cut(s)	749, 1288, 2274
BslI	CCNNNNNNNGG	5 cut(s)	213, 1318, 1403, 1990, 2072
BsmAI	GTCTC	5 cut(s)	21, 89, 305, 561, 587
BsmBI	CGTCTC	1 cut(s)	305
BsmFI	GGGAC	3 cut(s)	749, 1288, 2274
BsmI	GAATGC	1 cut(s)	1777
Bso31I	GGTCTC	2 cut(s)	89, 587
BsoBI	CYCGRG	2 cut(s)	1353, 1756
Bsp119I	TTCGAA	1 cut(s)	760
Bsp1286I	GDGCHC	5 cut(s)	159, 414, 427, 1167, 1458
Bsp143I	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
Bsp1720I	GCTNAGC	1 cut(s)	1943
Bsp19I	CCATGG	1 cut(s)	2223
BspACI	CCGC	5 cut(s)	207, 1509, 1711, 1961, 2264
BspCNI	CTCAG	8 cut(s)	426, 595, 817, 1528, 1583, 1810, 1956, 2242
BspLI	GGNNCC	9 cut(s)	318, 523, 737, 738, 967, 1245, 1554, 1762, 1851
BspMI	ACCTGC	1 cut(s)	5
BspPI	GGATC	7 cut(s)	53, 73, 261, 1804, 1844, 1857, 2176
BspT104I	TTCGAA	1 cut(s)	760
BspT107I	GGYRCC	3 cut(s)	521, 965, 1760
BspTI	CTTAAG	1 cut(s)	1898
BspTNI	GGTCTC	2 cut(s)	89, 587
BsrDI	GCAATG	2 cut(s)	1629, 1954
BsrI	ACTGG	6 cut(s)	274, 737, 970, 1114, 2132, 2357
BssECI	CCNNGG	6 cut(s)	99, 246, 525, 961, 1353, 2223
BssMI	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
BssNI	GRCGYC	1 cut(s)	296
BssT1I	CCWWGG	4 cut(s)	99, 525, 961, 2223
Bst2UI	CCWGG	4 cut(s)	1433, 1732, 1991, 2005
Bst4CI	ACNGT	6 cut(s)	148, 471, 537, 893, 1279, 1695
Bst6I	CTCTTC	3 cut(s)	170, 171, 585
BstACI	GRCGYC	1 cut(s)	296
BstAFI	CTTAAG	1 cut(s)	1898
BstAPI	GCANNNNNTGC	1 cut(s)	521
BstBI	TTCGAA	1 cut(s)	760
BstC8I	GCNNGC	3 cut(s)	480, 1576, 2266
BstDSI	CCRYGG	1 cut(s)	2223
BstEII	GGTNACC	2 cut(s)	335, 1706
BstHHI	GCGC	2 cut(s)	403, 2211
BstKTI	GATC	9 cut(s)	48, 81, 256, 721, 931, 1135, 1812, 1852, 2171
BstMAI	GTCTC	5 cut(s)	21, 89, 305, 561, 587
BstMBI	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
BstNI	CCWGG	4 cut(s)	1433, 1732, 1991, 2005
BstNSI	RCATGY	4 cut(s)	377, 808, 1578, 2058
BstPI	GGTNACC	2 cut(s)	335, 1706
BstSCI	CCNGG	7 cut(s)	339, 1352, 1353, 1431, 1730, 1989, 2003
BstSFI	CTRYAG	4 cut(s)	210, 1128, 1368, 1656
BstV1I	GCAGC	8 cut(s)	199, 229, 280, 1043, 1370, 1598, 1631, 2009
BstV2I	GAAGAC	1 cut(s)	1121
BstX2I	RGATCY	4 cut(s)	78, 928, 1809, 1849
BstXI	CCANNNNNNTGG	4 cut(s)	148, 977, 1254, 2230
BstYI	RGATCY	4 cut(s)	78, 928, 1809, 1849
Bsu36I	CCTNAGG	2 cut(s)	825, 2332
BtgI	CCRYGG	1 cut(s)	2223
BtgZI	GCGATG	2 cut(s)	168, 1092
BtsI	GCAGTG	4 cut(s)	120, 1165, 1515, 2218
BtsIMutI	CAGTG	8 cut(s)	120, 153, 289, 421, 533, 1165, 1515, 2218
BveI	ACCTGC	1 cut(s)	5
Cac8I	GCNNGC	3 cut(s)	480, 1576, 2266
CaiI	CAGNNNCTG	1 cut(s)	1109
CfoI	GCGC	2 cut(s)	403, 2211
Cfr13I	GGNCC	7 cut(s)	58, 317, 736, 1406, 1483, 2090, 2266
Cfr9I	CCCGGG	1 cut(s)	1353
CseI	GACGC	3 cut(s)	276, 285, 1019
Csp6I	GTAC	7 cut(s)	846, 1375, 1495, 1761, 1832, 2041, 2113
CspCI	CAANNNNNGTGG	2 cut(s)	2338, 2373
CviQI	GTAC	7 cut(s)	846, 1375, 1495, 1761, 1832, 2041, 2113
DpnI	GATC	9 cut(s)	47, 80, 255, 720, 930, 1134, 1811, 1851, 2170
DpnII	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
DrdI	GACNNNNNNGTC	1 cut(s)	1123
DseDI	GACNNNNNNGTC	1 cut(s)	1123
EaeI	YGGCCR	2 cut(s)	528, 2220
Eam1104I	CTCTTC	3 cut(s)	170, 171, 585
EarI	CTCTTC	3 cut(s)	170, 171, 585
Ecl136II	GAGCTC	1 cut(s)	157
Eco130I	CCWWGG	4 cut(s)	99, 525, 961, 2223
Eco147I	AGGCCT	1 cut(s)	996
Eco24I	GRGCYC	2 cut(s)	159, 414
Eco31I	GGTCTC	2 cut(s)	89, 587
Eco47I	GGWCC	2 cut(s)	736, 1406
Eco53kI	GAGCTC	1 cut(s)	157
Eco57I	CTGAAG	3 cut(s)	152, 1812, 1821
Eco81I	CCTNAGG	2 cut(s)	825, 2332
Eco88I	CYCGRG	2 cut(s)	1353, 1756
Eco91I	GGTNACC	2 cut(s)	335, 1706
EcoICRI	GAGCTC	1 cut(s)	157
EcoO109I	RGGNCCY	1 cut(s)	736
EcoO65I	GGTNACC	2 cut(s)	335, 1706
EcoRI	GAATTC	1 cut(s)	691
EcoRII	CCWGG	4 cut(s)	1431, 1730, 1989, 2003
EcoT14I	CCWWGG	4 cut(s)	99, 525, 961, 2223
EcoT22I	ATGCAT	4 cut(s)	801, 1447, 1605, 2311
EcoT38I	GRGCYC	2 cut(s)	159, 414
ErhI	CCWWGG	4 cut(s)	99, 525, 961, 2223
Esp3I	CGTCTC	1 cut(s)	305
FaqI	GGGAC	3 cut(s)	749, 1288, 2274
FauI	CCCGC	1 cut(s)	2257
FbaI	TGATCA	1 cut(s)	718
FblI	GTMKAC	1 cut(s)	1127
Fnu4HI	GCNGC	9 cut(s)	188, 218, 269, 1057, 1359, 1510, 1587, 1620, 2023
FriOI	GRGCYC	2 cut(s)	159, 414
Fsp4HI	GCNGC	9 cut(s)	188, 218, 269, 1057, 1359, 1510, 1587, 1620, 2023
GlaI	GCGC	2 cut(s)	402, 2210
GluI	GCNGC	9 cut(s)	188, 218, 269, 1057, 1359, 1510, 1587, 1620, 2023
GsaI	CCCAGC	2 cut(s)	195, 876
GsuI	CTGGAG	6 cut(s)	257, 561, 834, 854, 1192, 2115
HapII	CCGG	5 cut(s)	131, 341, 519, 1354, 2151
HgaI	GACGC	3 cut(s)	276, 285, 1019
HhaI	GCGC	2 cut(s)	403, 2211
Hin1I	GRCGYC	1 cut(s)	296
Hin6I	GCGC	2 cut(s)	401, 2209
HinP1I	GCGC	2 cut(s)	401, 2209
HindIII	AAGCTT	1 cut(s)	1878
HinfI	GANTC	3 cut(s)	750, 1035, 2243
HpaII	CCGG	5 cut(s)	131, 341, 519, 1354, 2151
HphI	GGTGA	9 cut(s)	29, 340, 347, 427, 552, 618, 992, 2052, 2090
Hpy166II	GTNNAC	7 cut(s)	151, 435, 560, 626, 664, 1128, 1832
Hpy188III	TCNNGA	8 cut(s)	540, 824, 851, 1104, 1847, 1918, 2172, 2323
Hpy8I	GTNNAC	7 cut(s)	151, 435, 560, 626, 664, 1128, 1832
Hpy99I	CGWCG	3 cut(s)	119, 301, 1035
HpyAV	CCTTC	6 cut(s)	316, 939, 1193, 1845, 1958, 2308
HpyCH4III	ACNGT	6 cut(s)	148, 471, 537, 893, 1279, 1695
Hsp92I	GRCGYC	1 cut(s)	296
HspAI	GCGC	2 cut(s)	401, 2209
KflI	GGGWCCC	1 cut(s)	736
KpnI	GGTACC	1 cut(s)	1764
Ksp22I	TGATCA	1 cut(s)	718
Kzo9I	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
LmnI	GCTCC	5 cut(s)	162, 276, 542, 829, 1461
Lsp1109I	GCAGC	8 cut(s)	199, 229, 280, 1043, 1370, 1598, 1631, 2009
MaeIII	GTNAC	9 cut(s)	62, 335, 887, 1175, 1339, 1706, 1999, 2078, 2240
MalI	GATC	9 cut(s)	47, 80, 255, 720, 930, 1134, 1811, 1851, 2170
MboI	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
MboII	GAAGA	9 cut(s)	158, 184, 187, 602, 1000, 1126, 1747, 1933, 2020
MflI	RGATCY	4 cut(s)	78, 928, 1809, 1849
MhlI	GDGCHC	5 cut(s)	159, 414, 427, 1167, 1458
MlsI	TGGCCA	2 cut(s)	530, 2222
MluCI	AATT	9 cut(s)	691, 722, 762, 1268, 1718, 1842, 1854, 1924, 2193
MluNI	TGGCCA	2 cut(s)	530, 2222
MlyI	GAGTC	1 cut(s)	2237
MmeI	TCCRAC	3 cut(s)	23, 838, 1631
Mox20I	TGGCCA	2 cut(s)	530, 2222
Mph1103I	ATGCAT	4 cut(s)	801, 1447, 1605, 2311
MroXI	GAANNNNTTC	1 cut(s)	622
MscI	TGGCCA	2 cut(s)	530, 2222
MseI	TTAA	5 cut(s)	1148, 1857, 1899, 2181, 2196
MslI	CAYNNNNRTG	5 cut(s)	390, 638, 2053, 2098, 2228
Msp20I	TGGCCA	2 cut(s)	530, 2222
MspA1I	CMGCKG	1 cut(s)	1509
MspCI	CTTAAG	1 cut(s)	1898
MspI	CCGG	5 cut(s)	131, 341, 519, 1354, 2151
MspR9I	CCNGG	7 cut(s)	341, 1354, 1355, 1433, 1732, 1991, 2005
Mva1269I	GAATGC	1 cut(s)	1777
MvaI	CCWGG	4 cut(s)	1433, 1732, 1991, 2005
NciI	CCSGG	3 cut(s)	341, 1354, 1355
NcoI	CCATGG	1 cut(s)	2223
NdeII	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
NlaIV	GGNNCC	9 cut(s)	318, 523, 737, 738, 967, 1245, 1554, 1762, 1851
NmuCI	GTSAC	5 cut(s)	62, 335, 1175, 2078, 2240
NsiI	ATGCAT	4 cut(s)	801, 1447, 1605, 2311
NspI	RCATGY	4 cut(s)	377, 808, 1578, 2058
NspV	TTCGAA	1 cut(s)	760
PaeI	GCATGC	1 cut(s)	1578
PaqCI	CACCTGC	1 cut(s)	5
PceI	AGGCCT	1 cut(s)	996
PciI	ACATGT	1 cut(s)	804
PctI	GAATGC	1 cut(s)	1777
PdmI	GAANNNNTTC	1 cut(s)	622
PfeI	GAWTC	2 cut(s)	750, 1035
PflMI	CCANNNNNTGG	1 cut(s)	1403
PfoI	TCCNGGA	1 cut(s)	1989
PkrI	GCNGC	9 cut(s)	189, 219, 270, 1058, 1360, 1511, 1588, 1621, 2024
PleI	GAGTC	1 cut(s)	2237
PpsI	GAGTC	1 cut(s)	2237
PpuMI	RGGWCCY	1 cut(s)	736
PscI	ACATGT	1 cut(s)	804
PshBI	ATTAAT	1 cut(s)	2196
PsiI	TTATAA	2 cut(s)	509, 1595
Psp124BI	GAGCTC	1 cut(s)	159
Psp5II	RGGWCCY	1 cut(s)	736
Psp6I	CCWGG	4 cut(s)	1431, 1730, 1989, 2003
PspEI	GGTNACC	2 cut(s)	335, 1706
PspFI	CCCAGC	2 cut(s)	191, 872
PspGI	CCWGG	4 cut(s)	1431, 1730, 1989, 2003
PspN4I	GGNNCC	9 cut(s)	318, 523, 737, 738, 967, 1245, 1554, 1762, 1851
PspPI	GGNCC	7 cut(s)	58, 317, 736, 1406, 1483, 2090, 2266
PspPPI	RGGWCCY	1 cut(s)	736
PstNI	CAGNNNCTG	1 cut(s)	1109
PsuI	RGATCY	4 cut(s)	78, 928, 1809, 1849
RsaI	GTAC	7 cut(s)	847, 1376, 1496, 1762, 1833, 2042, 2114
RsaNI	GTAC	7 cut(s)	846, 1375, 1495, 1761, 1832, 2041, 2113
RseI	CAYNNNNRTG	5 cut(s)	390, 638, 2053, 2098, 2228
SacI	GAGCTC	1 cut(s)	159
SaqAI	TTAA	5 cut(s)	1148, 1857, 1899, 2181, 2196
SatI	GCNGC	9 cut(s)	188, 218, 269, 1057, 1359, 1510, 1587, 1620, 2023
Sau3AI	GATC	9 cut(s)	45, 78, 253, 718, 928, 1132, 1809, 1849, 2168
Sau96I	GGNCC	7 cut(s)	58, 317, 736, 1406, 1483, 2090, 2266
SchI	GAGTC	1 cut(s)	2237
ScrFI	CCNGG	7 cut(s)	341, 1354, 1355, 1433, 1732, 1991, 2005
SduI	GDGCHC	5 cut(s)	159, 414, 427, 1167, 1458
SfcI	CTRYAG	4 cut(s)	210, 1128, 1368, 1656
SfuI	TTCGAA	1 cut(s)	760
SinI	GGWCC	2 cut(s)	736, 1406
SmaI	CCCGGG	1 cut(s)	1355
SmiMI	CAYNNNNRTG	5 cut(s)	390, 638, 2053, 2098, 2228
SmlI	CTYRAG	3 cut(s)	428, 1805, 1898
SmoI	CTYRAG	3 cut(s)	428, 1805, 1898
SphI	GCATGC	1 cut(s)	1578
Sse9I	AATT	9 cut(s)	691, 722, 762, 1268, 1718, 1842, 1854, 1924, 2193
SseBI	AGGCCT	1 cut(s)	996
SsiI	CCGC	5 cut(s)	207, 1509, 1711, 1961, 2264
SspI	AATATT	1 cut(s)	2299
SstI	GAGCTC	1 cut(s)	159
StuI	AGGCCT	1 cut(s)	996
StyD4I	CCNGG	7 cut(s)	339, 1352, 1353, 1431, 1730, 1989, 2003
StyI	CCWWGG	4 cut(s)	99, 525, 961, 2223
TaaI	ACNGT	6 cut(s)	148, 471, 537, 893, 1279, 1695
TaqI	TCGA	5 cut(s)	748, 760, 1033, 2246, 2312
TaqII	GACCGA	1 cut(s)	611
TasI	AATT	9 cut(s)	691, 722, 762, 1268, 1718, 1842, 1854, 1924, 2193
TatI	WGTACW	1 cut(s)	1374
TauI	GCSGC	1 cut(s)	1512
TfiI	GAWTC	2 cut(s)	750, 1035
Tru1I	TTAA	5 cut(s)	1148, 1857, 1899, 2181, 2196
Tru9I	TTAA	5 cut(s)	1148, 1857, 1899, 2181, 2196
TscAI	CASTG	8 cut(s)	127, 153, 289, 421, 540, 1172, 1522, 2218
TseFI	GTSAC	5 cut(s)	62, 335, 1175, 2078, 2240
TseI	GCWGC	8 cut(s)	187, 217, 268, 1056, 1358, 1586, 1619, 2022
Tsp45I	GTSAC	5 cut(s)	62, 335, 1175, 2078, 2240
TspDTI	ATGAA	4 cut(s)	492, 603, 660, 1001
TspGWI	ACGGA	2 cut(s)	81, 2097
TspMI	CCCGGG	1 cut(s)	1353
TspRI	CASTG	8 cut(s)	127, 153, 289, 421, 540, 1172, 1522, 2218
Van91I	CCANNNNNTGG	1 cut(s)	1403
Vha464I	CTTAAG	1 cut(s)	1898
VpaK11BI	GGWCC	2 cut(s)	736, 1406
VspI	ATTAAT	1 cut(s)	2196
XapI	RAATTY	3 cut(s)	691, 1268, 1924
XceI	RCATGY	4 cut(s)	377, 808, 1578, 2058
XcmI	CCANNNNNNNNNTGG	2 cut(s)	949, 1860
XmaI	CCCGGG	1 cut(s)	1353
XmiI	GTMKAC	1 cut(s)	1127
XmnI	GAANNNNTTC	1 cut(s)	622
Zsp2I	ATGCAT	4 cut(s)	801, 1447, 1605, 2311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.