MD01G1207000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001112
Physical Location & Seq
Forward (+)
30242148 .. 30248763
6616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1207000.v1.1.491

Sequence Viewer

Length: 3945 bp
ATGATGAATACAGTGCGTGTAGAACTTACAAAATCTATTAGTCCTGACTTGAACTGGAAGGTTTCAAAGGGTTATCGGAGTTCTTCAAGGCGGTCAAGGAAACCTGTTGACAAAAACCAAAAATTGGGTGCAGAGCTAGCTGAGAAAAGTGCTAGAAGTGCATCAGAAGCAACAGTTTCTGAATCAGACAAGGGTGTGGCTGTATGTGGGAGACGATTGGGTGACAAAATTGAGCATGTACCGATTAAGAAACGGAGACTTATGGTCCGGTCTCCATCACCCCCTCCACACCTTGAAGACAACAAACCACTATTGGATGGCCGTCATTCTTCAGGTCATAAGTCTTGTGCAAAATCAGTTGGCAAAAAGCATCCCACAAGGAGTGATACGTCTACCCTGACTAGGGTCAGCCATAATATAGCCGGTAGCGGTGTTATTGAGAATTTGAATGAGGTGACAAATCAAAAGCCTGGTGACGTGGATGACTTCTCCGGTATTGAGATATTGGCAGCTGCTGCCTGCAACAACAGCATAAATGATGATATCAATCATGCTGTGAAGAATCAAGTGGGTGAAGATTCATCTCGGGATGCAAAAGATGCATCAACGTCTGCAAGGCCCTTGGAGCAAACCACCAATGCGTCAACTTCTTCAACAGTCATGAGAACTGCTTCAGAATTTTCAGAAGCAAGAGATGCATCAGTCTCTGCAATCTTGGAAGAAAGTAGTGCGTCATTGGAAACAGTACATTCATCCCCTAAAGATGTACGACGAGAAGATAAGGTAGGGAGCTCCTCTTTTGAGGCTGATGGGATTAATACCACCAAAGCACAGGACGAAGCAGAAGCAAGATCCAGCTCCTCAAAAGATGTTAGGTTCCACTGGGACCTAAATGTTGGTATAGATGCTTGGGAGGAACCTTGTGATATGGCGATTGCTGATCCTCAAACAACTGCTGCAGATGATATCTCCATGGATAATAAGCAGGGTGGTGCAAATTTCCAGGCTTCAGAAGCTAATGAGATACCAAAGGAGGAAGATGCAAAGAATGACATTGCAAGCACAGTGAAGAAGCCCATGTCTGACAATGAGGAAGAGGGATTGAAAGCGTGCCCTGAATTTGAGTTGCGTTATGGTAAATTTGTTTCTACTGATAATGCTTTGGGATCTTCAAAAGATTCTGGTAGCAGTGCAAAAGCTTCCTCTCAGGATGCCAGTGTGGATGCATGCATTGATCCTTCTCTTTGCCCTGAGTTTGAGTTGAGTTATGGTAAATGTGTTTCTACTGATAATGCTTTGGGATCTTCAAAAGATTCTGGTAGCAGTGCAAAAGCTTCCTCTGAGGATGCCAGTGTGGATGCATGCATTGATCGTTCTCCTTGTGGCATCGCAGTCACATGCCCTGTGTCTGAGGAGACCGACAAAACACCGATTTCCAGCTTTCCTGTTAAGCATATGACAGGAGATACTGCTTCTGGTGAACTACTGGGTGAAACTGTCTGTTCAGAGAGTGTTAAGGTTGAAAACCCTGCTTTAGCTTGTGTGCCAGAGGGGGCACCTTGTGAGATTGAAGGCACTGTTCTAGATGAAGATGGGAAGTGCTCTGGTGCAACATCTAGCGTCCATGATGATCCAGAATCTCCGGAAGAGACGAAGGGTGTGGAAAGCTGTCAGTCACTCTCTCCCGTTCTCCTTGATGTCAAGCCTGTAGCTAAAGCAGAAGATGTGGCTATTCACCATTCCAAACTTGATGTCAAGCCTGTAGCTAAAACAGAGGATGTGGCTGTTCACCATTCCAAGCTGGATGTTCAGCCTGTAGCTAAAACAGAGGATGTGGCTATTCACCATTCCAAACATGATTCTAATGATAAATCTGCATCTGGTGCAGCTGTTGGAGAAGGCCGATCTCTGGTGACTGTAATTGCAAAGCAGCCAGTAGAAGCAGCCTCTGACACTCATACTGTTGATTCTCTGCCAAATGATGGTTCTGCGGAAGTAGTGCATAAACCTTCAGGGAATCCCATGATGAATCCAGCAGCTACTGCTGGGTCTTCTCTTGAGCAATGCCACTATGGTGAAGGTACCTCACGTAGCTCGGGTAGAGCCACTGAAGATCCTTCCAGTGATGATTATGACTTGAATACTCGTCAAGATGATAATGATCACATGGTTGGCGAGGGAAACACTAAGGAACCTGAAGCTGGTTACGACTCTCAGTATGAAGATGGGGAGCTGAGGGAGTCAGATGTACCATATTGGGAGGAGAATGAAATTGATGACTTAGAAGTTGAATGTGTTGACTATGGTTCTGATACATGTGACAGTGAAGCTGCTGATGACTCTGTATCAGGGAAAGTTGGAATGGAACTTGAATGCAGAGAAACTGAATTATTTGGGGAGTCGAGGAAAATTAACAGTAACATGAAGCTTGTGAGAGGATTGAGTCCAGGATCTGATAATACGTGTGAGAAAAATGAACATGCTTTGAGACAATGTTCCGTTGGCTCGAAGACGAAAACTTCTGGATCTGATCAATTACCTGGTGATTCTGAAGCTTCTTCAAACAGAACTGCAGAAGCAATTGAGGGGTGCACTGTAAGGAGACATGCTGTTAATAGCTTCGATTGTCATGATGCCAAACATTCTCCTGCCAACGTGGTTGGATCAATGGCATCTGACTCCTCAAATAAGATGGGTACTGAATGTGCAAGAAGGAGGAGGTTGGGCAACTTTGATTCTATTCGCTCTGAGGAAGCTGGTTCCGATCAGTCCATGGGAAGGGAAAAATCTGATTCACGTATGCAGGGTAAAAGTTTCGGAGGTGTGGTTAATTCTTCGGGGAGTTATTGGGATTCCAAGCGCCGCGAATCACCCACTTATCGTGGTTCCTTTGGTTCTGGACGCTCTAGACCAAGAATTGTTGTTGAGAACCATGGTTATGAGATGGAATCAGACGTCACATTTTCAGACGCAGCAGGAGTTCACAACCGTGTTCGCAGGCAAGCTATTACTTTTTCATCAAATCGTTCGTATCATCCCGCGTTTAGAAGATCATCGCCATCTGAAAGGAATGATGCACACAACATACATAGGGGAATGATACCTATGAGAGATACAAGTCCTGATAGACGGAGGTTTAGACGATATCCGCAAGGGGTTAACAGAGGCATCAGGGAGGAGTACCACAGGCCCATACCCGATGATCCCAACGAATGCTCTTATAATGTGCCACGACGAATGCCCAGGAGAGAACAAAGCACTTCACCCCCTGGCAGAGGACCTATATATTATAGTAGACCCTACCAGAAGCCCCAGTCTCGATGCAGAAGTCGCTCTCCTCTTGGATGGGGTTTACCGAGAGAACGGAATGATATTTCAAGACATCGTGGTAGCAGGTCTCCTGATTATAGGTTTGATTCTAATATGGAGCTGAGGGTGCCTTTCCAAAGGCAAAATTTCGGAGGCAAGTATGATGTAGGGTTCGTGTCCCCACCAAAGCGTCGGTTTTCCCCACAACAGAATTCCAGATGGTTTGATGATTCACATAGAGGTGTAGATCACAATTTCAGGGGCGGGAGATTTGCCGGAAGACGGTTCCAGCCAGGACAGAGGTTTGACTCAGAGCGTTCATCTCGGAGATTGAATCAAGATGGTTACTCTGAACCCGTGATGCGTCCTGCAAGATACTCTGAGTTGCCCAGTGGTGGAAGGGAGTGTAGATACGAAGGTAGTGATGATGATAGAAGGAAACCTGATGGAAGATATGAGATTGTTCATCGCGTGAGGCGTTTTGATTCAGATGGTGGTGTGCGTCAGTACCGTTATGATGAAGATCGTTTTGCATCTCACAACACGCAGAACTACGATGAATCTGACCATAGGGCGGCCGAGAGGCGGCCTCGAGAAGCTTACGTAGGAGAAGTAGCGAAGAGGAGAGTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1315

Amino Acids

144.11

Weight (kDa)

5.65

Isoelectric Point (pI)

65.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3222
AatII GACGTC 1 cut(s) 2958
Acc36I ACCTGC 1 cut(s) 3384
Acc65I GGTACC 1 cut(s) 2081
AccB1I GGYRCC 3 cut(s) 1555, 2081, 3436
AccB7I CCANNNNNTGG 2 cut(s) 124, 1982
AccI GTMKAC 2 cut(s) 392, 3295
AccII CGCG 3 cut(s) 2865, 3041, 3780
AccIII TCCGGA 1 cut(s) 1642
AciI CCGC 9 cut(s) 91, 429, 1991, 2863, 3039, 3149, 3573, 3884, 3895
AcoI YGGCCR 2 cut(s) 319, 3885
AcsI RAATTY 7 cut(s) 442, 677, 997, 1118, 1139, 3454, 3520
AcuI CTGAAG 7 cut(s) 315, 657, 993, 1995, 2130, 2217, 2571
AcyI GRCGYC 1 cut(s) 2955
AdeI CACNNNGTG 1 cut(s) 1562
AfaI GTAC 8 cut(s) 240, 747, 768, 2083, 2250, 2698, 3182, 3818
AflIII ACRYGT 2 cut(s) 2315, 2462
AhdI GACNNNNNGTC 1 cut(s) 263
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 7 cut(s) 469, 1002, 2446, 2539, 3242, 3268, 3601
AjuI GAANNNNNNNTTGG 2 cut(s) 1969, 2001
AleI CACNNNNGTG 2 cut(s) 2073, 2988
Alw21I GWGCWC 3 cut(s) 794, 1604, 2594
Alw44I GTGCAC 1 cut(s) 2590
AlwNI CAGNNNCTG 6 cut(s) 179, 515, 707, 1949, 2042, 2453
Ama87I CYCGRG 3 cut(s) 585, 2095, 3900
Aor13HI TCCGGA 1 cut(s) 1642
AoxI GGCC 6 cut(s) 319, 617, 1900, 3188, 3885, 3896
ApaLI GTGCAC 1 cut(s) 2590
ApoI RAATTY 7 cut(s) 442, 677, 997, 1118, 1139, 3454, 3520
ArsI GACNNNNNNTTYG 2 cut(s) 1737, 1769
AseI ATTAAT 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 670
Asp718I GGTACC 1 cut(s) 2081
AspLEI GCGC 1 cut(s) 2862
AspS9I GGNCC 5 cut(s) 265, 618, 886, 3189, 3278
AsuNHI GCTAGC 1 cut(s) 136
AvaI CYCGRG 3 cut(s) 585, 2095, 3900
AvaII GGWCC 3 cut(s) 265, 886, 3278
BaeGI GKGCMC 3 cut(s) 1115, 1558, 2594
BaeI ACNNNNGTAYC 2 cut(s) 3712, 3745
BanI GGYRCC 3 cut(s) 1555, 2081, 3436
BanII GRGCYC 1 cut(s) 794
BbsI GAAGAC 4 cut(s) 303, 2043, 2516, 3595
Bbv12I GWGCWC 3 cut(s) 794, 1604, 2594
BbvCI CCTCAGC 2 cut(s) 2234, 3431
BceAI ACGGC 1 cut(s) 306
BciT130I CCWGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
BclI TGATCA 2 cut(s) 2161, 2530
BfaI CTAG 6 cut(s) 137, 153, 402, 1583, 1617, 2907
BfmI CTRYAG 5 cut(s) 957, 1707, 1761, 1815, 2571
BfoI RGCGCY 1 cut(s) 2863
BfuAI ACCTGC 1 cut(s) 3384
Bme1390I CCNGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
Bme18I GGWCC 3 cut(s) 265, 886, 3278
BmeRI GACNNNNNGTC 1 cut(s) 263
BmeT110I CYCGRG 3 cut(s) 585, 2095, 3900
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 5 cut(s) 265, 618, 886, 3189, 3278
BmrFI CCNGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
BmrI ACTGGG 4 cut(s) 892, 1496, 3307, 3693
BmtI GCTAGC 1 cut(s) 140
BmuI ACTGGG 4 cut(s) 892, 1496, 3307, 3693
BoxI GACNNNNGTC 1 cut(s) 404
BpiI GAAGAC 4 cut(s) 303, 2043, 2516, 3595
BplI GAGNNNNNCTC 2 cut(s) 3883, 3915
Bpu10I CCTNAGC 2 cut(s) 2234, 3431
BpuEI CTTGAG 1 cut(s) 2078
BsaAI YACGTR 4 cut(s) 2090, 2463, 2798, 3913
BsaBI GATNNNNATC 3 cut(s) 546, 2160, 3831
BsaHI GRCGYC 1 cut(s) 2955
BsaI GGTCTC 3 cut(s) 276, 1409, 3402
BsaJI CCNNGG 6 cut(s) 621, 972, 2772, 2932, 3242, 3268
BsaWI WCCGGW 3 cut(s) 267, 491, 1642
BsaXI ACNNNNNCTCC 2 cut(s) 2811, 2841
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 9 cut(s) 59, 887, 1215, 1350, 1491, 1934, 2121, 3313, 3699
Bse3DI GCAATG 2 cut(s) 1053, 2069
Bse8I GATNNNNATC 3 cut(s) 546, 2160, 3831
BseAI TCCGGA 1 cut(s) 1642
BseBI CCWGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
BseDI CCNNGG 6 cut(s) 621, 972, 2772, 2932, 3242, 3268
BseJI GATNNNNATC 3 cut(s) 546, 2160, 3831
BseMI GCAATG 2 cut(s) 1053, 2069
BseNI ACTGG 9 cut(s) 59, 887, 1215, 1350, 1491, 1934, 2121, 3313, 3699
BseRI GAGGAG 8 cut(s) 784, 850, 1427, 2276, 2671, 2731, 3191, 3327
BseSI GKGCMC 3 cut(s) 1115, 1558, 2594
BseX3I CGGCCG 1 cut(s) 3885
BseYI CCCAGC 1 cut(s) 2045
BsgI GTGCAG 2 cut(s) 150, 1905
Bsh1236I CGCG 3 cut(s) 2865, 3041, 3780
Bsh1285I CGRYCG 1 cut(s) 3888
BshFI GGCC 6 cut(s) 321, 619, 1902, 3190, 3887, 3898
BshNI GGYRCC 3 cut(s) 1555, 2081, 3436
BsiEI CGRYCG 1 cut(s) 3888
BsiHKAI GWGCWC 3 cut(s) 794, 1604, 2594
BsiHKCI CYCGRG 3 cut(s) 585, 2095, 3900
BsiSI CCGG 5 cut(s) 268, 423, 492, 1643, 3585
BslFI GGGAC 2 cut(s) 899, 3472
BsmBI CGTCTC 2 cut(s) 205, 1643
BsmFI GGGAC 2 cut(s) 899, 3472
BsmI GAATGC 3 cut(s) 2378, 3218, 3243
BsnI GGCC 6 cut(s) 321, 619, 1902, 3190, 3887, 3898
Bso31I GGTCTC 3 cut(s) 276, 1409, 3402
BsoBI CYCGRG 3 cut(s) 585, 2095, 3900
Bsp1286I GDGCHC 5 cut(s) 794, 1115, 1558, 1604, 2594
Bsp13I TCCGGA 1 cut(s) 1642
Bsp19I CCATGG 3 cut(s) 972, 2772, 2932
BspACI CCGC 9 cut(s) 91, 429, 1991, 2863, 3039, 3149, 3573, 3884, 3895
BspANI GGCC 6 cut(s) 321, 619, 1902, 3190, 3887, 3898
BspEI TCCGGA 1 cut(s) 1642
BspFNI CGCG 3 cut(s) 2865, 3041, 3780
BspHI TCATGA 2 cut(s) 660, 2629
BspMAI CTGCAG 2 cut(s) 961, 2575
BspMI ACCTGC 1 cut(s) 3384
BspOI GCTAGC 1 cut(s) 140
BspT107I GGYRCC 3 cut(s) 1555, 2081, 3436
BspTNI GGTCTC 3 cut(s) 276, 1409, 3402
BsrDI GCAATG 2 cut(s) 1053, 2069
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 9 cut(s) 59, 887, 1215, 1350, 1491, 1934, 2121, 3313, 3699
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 6 cut(s) 621, 972, 2772, 2932, 3242, 3268
BssNI GRCGYC 1 cut(s) 2955
BssT1I CCWWGG 4 cut(s) 621, 972, 2772, 2932
Bst2UI CCWGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
Bst6I CTCTTC 3 cut(s) 1089, 1641, 3923
BstACI GRCGYC 1 cut(s) 2955
BstAPI GCANNNNNTGC 5 cut(s) 515, 599, 695, 1883, 2042
BstBAI YACGTR 4 cut(s) 2090, 2463, 2798, 3913
BstC8I GCNNGC 8 cut(s) 138, 520, 1060, 1111, 1228, 1363, 2999, 3003
BstDSI CCRYGG 3 cut(s) 972, 2772, 2932
BstENI CCTNNNNNAGG 1 cut(s) 3273
BstFNI CGCG 3 cut(s) 2865, 3041, 3780
BstH2I RGCGCY 1 cut(s) 2863
BstHHI GCGC 1 cut(s) 2862
BstMCI CGRYCG 1 cut(s) 3888
BstNI CCWGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
BstNSI RCATGY 7 cut(s) 239, 1230, 1365, 1401, 2319, 2483, 2609
BstPAI GACNNNNGTC 1 cut(s) 404
BstSCI CCNGG 7 cut(s) 469, 1002, 2446, 2539, 3242, 3268, 3601
BstSFI CTRYAG 5 cut(s) 957, 1707, 1761, 1815, 2571
BstSLI GKGCMC 3 cut(s) 1115, 1558, 2594
BstSNI TACGTA 1 cut(s) 3913
BstUI CGCG 3 cut(s) 2865, 3041, 3780
BstV2I GAAGAC 4 cut(s) 303, 2043, 2516, 3595
BstX2I RGATCY 6 cut(s) 851, 1166, 1301, 2113, 2450, 2525
BstYI RGATCY 6 cut(s) 851, 1166, 1301, 2113, 2450, 2525
BstZI CGGCCG 1 cut(s) 3885
BsuRI GGCC 6 cut(s) 321, 619, 1902, 3190, 3887, 3898
BtgI CCRYGG 3 cut(s) 972, 2772, 2932
BtgZI GCGATG 3 cut(s) 1372, 3039, 3761
BtrI CACGTC 1 cut(s) 478
BtsI GCAGTG 2 cut(s) 1195, 1330
BveI ACCTGC 1 cut(s) 3384
Cac8I GCNNGC 8 cut(s) 138, 520, 1060, 1111, 1228, 1363, 2999, 3003
CaiI CAGNNNCTG 6 cut(s) 179, 515, 707, 1949, 2042, 2453
CciI TCATGA 2 cut(s) 660, 2629
CfoI GCGC 1 cut(s) 2862
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 5 cut(s) 265, 618, 886, 3189, 3278
CseI GACGC 8 cut(s) 630, 720, 1609, 2910, 2978, 3488, 3662, 3800
CsiI ACCWGGT 1 cut(s) 2539
Csp6I GTAC 8 cut(s) 239, 746, 767, 2082, 2249, 2697, 3181, 3817
CviQI GTAC 8 cut(s) 239, 746, 767, 2082, 2249, 2697, 3181, 3817
DraIII CACNNNGTG 1 cut(s) 1562
DriI GACNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 2 cut(s) 319, 3885
EagI CGGCCG 1 cut(s) 3885
Eam1104I CTCTTC 3 cut(s) 1089, 1641, 3923
Eam1105I GACNNNNNGTC 1 cut(s) 263
EarI CTCTTC 3 cut(s) 1089, 1641, 3923
Ecl136II GAGCTC 1 cut(s) 792
EclXI CGGCCG 1 cut(s) 3885
Eco105I TACGTA 1 cut(s) 3913
Eco130I CCWWGG 4 cut(s) 621, 972, 2772, 2932
Eco24I GRGCYC 1 cut(s) 794
Eco31I GGTCTC 3 cut(s) 276, 1409, 3402
Eco32I GATATC 3 cut(s) 544, 967, 3146
Eco47I GGWCC 3 cut(s) 265, 886, 3278
Eco52I CGGCCG 1 cut(s) 3885
Eco53kI GAGCTC 1 cut(s) 792
Eco57I CTGAAG 7 cut(s) 315, 657, 993, 1995, 2130, 2217, 2571
Eco88I CYCGRG 3 cut(s) 585, 2095, 3900
EcoICRI GAGCTC 1 cut(s) 792
EcoNI CCTNNNNNAGG 1 cut(s) 3273
EcoO109I RGGNCCY 3 cut(s) 618, 886, 3278
EcoRI GAATTC 1 cut(s) 3520
EcoRII CCWGG 7 cut(s) 469, 1002, 2446, 2539, 3242, 3268, 3601
EcoRV GATATC 3 cut(s) 544, 967, 3146
EcoT14I CCWWGG 4 cut(s) 621, 972, 2772, 2932
EcoT22I ATGCAT 6 cut(s) 604, 700, 1228, 1232, 1363, 1367
EcoT38I GRGCYC 1 cut(s) 794
ErhI CCWWGG 4 cut(s) 621, 972, 2772, 2932
Esp3I CGTCTC 2 cut(s) 205, 1643
FaqI GGGAC 2 cut(s) 899, 3472
FauI CCCGC 2 cut(s) 3046, 3566
FauNDI CATATG 1 cut(s) 1455
FbaI TGATCA 2 cut(s) 2161, 2530
FblI GTMKAC 2 cut(s) 392, 3295
FriOI GRGCYC 1 cut(s) 794
FspBI CTAG 6 cut(s) 137, 153, 402, 1583, 1617, 2907
GlaI GCGC 1 cut(s) 2861
GsaI CCCAGC 1 cut(s) 2049
HaeII RGCGCY 1 cut(s) 2863
HaeIII GGCC 6 cut(s) 321, 619, 1902, 3190, 3887, 3898
HapII CCGG 5 cut(s) 268, 423, 492, 1643, 3585
HgaI GACGC 8 cut(s) 630, 720, 1609, 2910, 2978, 3488, 3662, 3800
HhaI GCGC 1 cut(s) 2862
Hin1I GRCGYC 1 cut(s) 2955
Hin6I GCGC 1 cut(s) 2860
HinP1I GCGC 1 cut(s) 2860
HincII GTYRAC 4 cut(s) 109, 645, 2299, 3160
HindII GTYRAC 4 cut(s) 109, 645, 2299, 3160
HindIII AAGCTT 5 cut(s) 1197, 1332, 2426, 2553, 3906
HpaI GTTAAC 1 cut(s) 3160
HpaII CCGG 5 cut(s) 268, 423, 492, 1643, 3585
Hpy99I CGWCG 3 cut(s) 774, 3237, 3504
HpyCH4IV ACGT 9 cut(s) 389, 477, 608, 2089, 2462, 2655, 2797, 2955, 3912
HpySE526I ACGT 9 cut(s) 389, 477, 608, 2089, 2462, 2655, 2797, 2955, 3912
Hsp92I GRCGYC 1 cut(s) 2955
HspAI GCGC 1 cut(s) 2860
Kpn2I TCCGGA 1 cut(s) 1642
KpnI GGTACC 1 cut(s) 2085
Ksp22I TGATCA 2 cut(s) 2161, 2530
KspAI GTTAAC 1 cut(s) 3160
LmnI GCTCC 6 cut(s) 625, 789, 797, 863, 2230, 3427
MabI ACCWGGT 1 cut(s) 2539
MaeI CTAG 6 cut(s) 137, 153, 402, 1583, 1617, 2907
MaeII ACGT 9 cut(s) 389, 477, 608, 2089, 2462, 2655, 2797, 2955, 3912
MfeI CAATTG 1 cut(s) 2580
MflI RGATCY 6 cut(s) 851, 1166, 1301, 2113, 2450, 2525
MhlI GDGCHC 5 cut(s) 794, 1115, 1558, 1604, 2594
MlyI GAGTC 7 cut(s) 2204, 2249, 2333, 2408, 2452, 2672, 3611
MmeI TCCRAC 3 cut(s) 1873, 2338, 2641
Mph1103I ATGCAT 6 cut(s) 604, 700, 1228, 1232, 1363, 1367
MroI TCCGGA 1 cut(s) 1642
MroXI GAANNNNTTC 1 cut(s) 670
MseI TTAA 9 cut(s) 246, 816, 1449, 1515, 2412, 2613, 2829, 3159, 3943
MslI CAYNNNNRTG 4 cut(s) 2073, 2937, 2988, 3549
MspA1I CMGCKG 2 cut(s) 512, 1889
MspI CCGG 5 cut(s) 268, 423, 492, 1643, 3585
MspR9I CCNGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
MunI CAATTG 1 cut(s) 2580
Mva1269I GAATGC 3 cut(s) 2378, 3218, 3243
MvaI CCWGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
MvnI CGCG 3 cut(s) 2865, 3041, 3780
NcoI CCATGG 3 cut(s) 972, 2772, 2932
NdeI CATATG 1 cut(s) 1455
NheI GCTAGC 1 cut(s) 136
NmeAIII GCCGAG 1 cut(s) 3913
NmuCI GTSAC 8 cut(s) 221, 454, 473, 1393, 1674, 1912, 2318, 2956
NsiI ATGCAT 6 cut(s) 604, 700, 1228, 1232, 1363, 1367
NspI RCATGY 7 cut(s) 239, 1230, 1365, 1401, 2319, 2483, 2609
OliI CACNNNNGTG 2 cut(s) 2073, 2988
PaeI GCATGC 2 cut(s) 1230, 1365
PaeR7I CTCGAG 1 cut(s) 3900
PagI TCATGA 2 cut(s) 660, 2629
PciI ACATGT 1 cut(s) 2315
PcsI WCGNNNNNNNCGW 1 cut(s) 3784
PctI GAATGC 3 cut(s) 2378, 3218, 3243
PdmI GAANNNNTTC 1 cut(s) 670
PflMI CCANNNNNTGG 2 cut(s) 124, 1982
PfoI TCCNGGA 1 cut(s) 2446
PleI GAGTC 7 cut(s) 2204, 2248, 2333, 2407, 2451, 2672, 3611
PpsI GAGTC 7 cut(s) 2204, 2248, 2333, 2407, 2451, 2672, 3611
Ppu21I YACGTR 4 cut(s) 2090, 2463, 2798, 3913
PpuMI RGGWCCY 2 cut(s) 886, 3278
PscI ACATGT 1 cut(s) 2315
PshAI GACNNNNGTC 1 cut(s) 404
PshBI ATTAAT 1 cut(s) 816
PsiI TTATAA 1 cut(s) 3222
Psp124BI GAGCTC 1 cut(s) 794
Psp5II RGGWCCY 2 cut(s) 886, 3278
Psp6I CCWGG 7 cut(s) 469, 1002, 2446, 2539, 3242, 3268, 3601
PspFI CCCAGC 1 cut(s) 2045
PspGI CCWGG 7 cut(s) 469, 1002, 2446, 2539, 3242, 3268, 3601
PspPI GGNCC 5 cut(s) 265, 618, 886, 3189, 3278
PspPPI RGGWCCY 2 cut(s) 886, 3278
PstI CTGCAG 2 cut(s) 961, 2575
PstNI CAGNNNCTG 6 cut(s) 179, 515, 707, 1949, 2042, 2453
PsuI RGATCY 6 cut(s) 851, 1166, 1301, 2113, 2450, 2525
PvuII CAGCTG 2 cut(s) 512, 1889
RsaI GTAC 8 cut(s) 240, 747, 768, 2083, 2250, 2698, 3182, 3818
RsaNI GTAC 8 cut(s) 239, 746, 767, 2082, 2249, 2697, 3181, 3817
RseI CAYNNNNRTG 4 cut(s) 2073, 2937, 2988, 3549
SacI GAGCTC 1 cut(s) 794
SaqAI TTAA 9 cut(s) 246, 816, 1449, 1515, 2412, 2613, 2829, 3159, 3943
Sau96I GGNCC 5 cut(s) 265, 618, 886, 3189, 3278
SchI GAGTC 7 cut(s) 2204, 2249, 2333, 2408, 2452, 2672, 3611
ScrFI CCNGG 7 cut(s) 471, 1004, 2448, 2541, 3244, 3270, 3603
SduI GDGCHC 5 cut(s) 794, 1115, 1558, 1604, 2594
SexAI ACCWGGT 1 cut(s) 2539
SfcI CTRYAG 5 cut(s) 957, 1707, 1761, 1815, 2571
Sfr274I CTCGAG 1 cut(s) 3900
SinI GGWCC 3 cut(s) 265, 886, 3278
SlaI CTCGAG 1 cut(s) 3900
SmiMI CAYNNNNRTG 4 cut(s) 2073, 2937, 2988, 3549
SmlI CTYRAG 2 cut(s) 2057, 3900
SmoI CTYRAG 2 cut(s) 2057, 3900
SnaBI TACGTA 1 cut(s) 3913
SphI GCATGC 2 cut(s) 1230, 1365
SsiI CCGC 9 cut(s) 91, 429, 1991, 2863, 3039, 3149, 3573, 3884, 3895
SspMI CTAG 6 cut(s) 137, 153, 402, 1583, 1617, 2907
SstI GAGCTC 1 cut(s) 794
StyD4I CCNGG 7 cut(s) 469, 1002, 2446, 2539, 3242, 3268, 3601
StyI CCWWGG 4 cut(s) 621, 972, 2772, 2932
TaiI ACGT 9 cut(s) 392, 480, 611, 2092, 2465, 2658, 2800, 2958, 3915
TaqI TCGA 5 cut(s) 2402, 2507, 2622, 3319, 3901
TaqII GACCGA 1 cut(s) 1433
TatI WGTACW 1 cut(s) 745
TauI GCSGC 3 cut(s) 2865, 3887, 3898
Tru1I TTAA 9 cut(s) 246, 816, 1449, 1515, 2412, 2613, 2829, 3159, 3943
Tru9I TTAA 9 cut(s) 246, 816, 1449, 1515, 2412, 2613, 2829, 3159, 3943
TseFI GTSAC 8 cut(s) 221, 454, 473, 1393, 1674, 1912, 2318, 2956
Tsp45I GTSAC 8 cut(s) 221, 454, 473, 1393, 1674, 1912, 2318, 2956
TspGWI ACGGA 4 cut(s) 268, 2488, 3145, 3379
Van91I CCANNNNNTGG 2 cut(s) 124, 1982
VneI GTGCAC 1 cut(s) 2590
VpaK11BI GGWCC 3 cut(s) 265, 886, 3278
VspI ATTAAT 1 cut(s) 816
XagI CCTNNNNNAGG 1 cut(s) 3273
XapI RAATTY 7 cut(s) 442, 677, 997, 1118, 1139, 3454, 3520
XbaI TCTAGA 2 cut(s) 1582, 2906
XceI RCATGY 7 cut(s) 239, 1230, 1365, 1401, 2319, 2483, 2609
XhoI CTCGAG 1 cut(s) 3900
XmiI GTMKAC 2 cut(s) 392, 3295
XmnI GAANNNNTTC 1 cut(s) 670
XspI CTAG 6 cut(s) 137, 153, 402, 1583, 1617, 2907
ZraI GACGTC 1 cut(s) 2956
Zsp2I ATGCAT 6 cut(s) 604, 700, 1228, 1232, 1363, 1367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.