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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2643 |
| AarI | CACCTGC | 2 cut(s) | 1100, 1246 |
| Acc36I | ACCTGC | 3 cut(s) | 1100, 1246, 2961 |
| Acc65I | GGTACC | 1 cut(s) | 3523 |
| AccB1I | GGYRCC | 1 cut(s) | 3523 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2537 |
| AccI | GTMKAC | 2 cut(s) | 906, 2890 |
| AccIII | TCCGGA | 1 cut(s) | 1420 |
| AciI | CCGC | 8 cut(s) | 128, 1436, 2001, 2521, 2591, 2773, 3358, 3424 |
| AcoI | YGGCCR | 1 cut(s) | 2518 |
| AcsI | RAATTY | 3 cut(s) | 31, 1895, 1994 |
| AcuI | CTGAAG | 5 cut(s) | 330, 393, 2196, 3348, 3393 |
| AfaI | GTAC | 8 cut(s) | 15, 148, 204, 720, 1723, 1875, 2034, 3525 |
| AflIII | ACRYGT | 2 cut(s) | 1912, 1934 |
| AhlI | ACTAGT | 1 cut(s) | 416 |
| AjiI | CACGTC | 1 cut(s) | 2831 |
| AjnI | CCWGG | 5 cut(s) | 322, 478, 759, 1074, 2840 |
| AleI | CACNNNNGTG | 2 cut(s) | 1713, 2616 |
| Alw21I | GWGCWC | 3 cut(s) | 85, 1255, 2805 |
| Alw26I | GTCTC | 2 cut(s) | 1629, 2107 |
| Alw44I | GTGCAC | 2 cut(s) | 81, 1251 |
| Ama87I | CYCGRG | 1 cut(s) | 3477 |
| Aor13HI | TCCGGA | 1 cut(s) | 1420 |
| AoxI | GGCC | 7 cut(s) | 602, 1072, 1537, 2251, 2518, 2786, 2825 |
| ApaLI | GTGCAC | 2 cut(s) | 81, 1251 |
| ApoI | RAATTY | 3 cut(s) | 31, 1895, 1994 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 693, 999 |
| Asp718I | GGTACC | 1 cut(s) | 3523 |
| AspA2I | CCTAGG | 2 cut(s) | 2056, 3490 |
| AspLEI | GCGC | 2 cut(s) | 2858, 3464 |
| AspS9I | GGNCC | 7 cut(s) | 113, 265, 603, 1685, 2825, 2876, 2973 |
| AsuC2I | CCSGG | 3 cut(s) | 1043, 2166, 2868 |
| AvaI | CYCGRG | 1 cut(s) | 3477 |
| AvaII | GGWCC | 5 cut(s) | 113, 265, 1685, 2876, 2973 |
| AvrII | CCTAGG | 2 cut(s) | 2056, 3490 |
| BaeGI | GKGCMC | 3 cut(s) | 85, 1255, 3021 |
| BamHI | GGATCC | 3 cut(s) | 971, 1666, 2566 |
| BanI | GGYRCC | 1 cut(s) | 3523 |
| BanII | GRGCYC | 2 cut(s) | 1382, 2805 |
| BauI | CACGAG | 2 cut(s) | 570, 1697 |
| BbsI | GAAGAC | 2 cut(s) | 217, 798 |
| Bbv12I | GWGCWC | 3 cut(s) | 85, 1255, 2805 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1283, 1317 |
| BciT130I | CCWGG | 5 cut(s) | 324, 480, 761, 1076, 2842 |
| BcnI | CCSGG | 3 cut(s) | 1043, 2166, 2868 |
| BcoDI | GTCTC | 2 cut(s) | 1629, 2107 |
| BcuI | ACTAGT | 1 cut(s) | 416 |
| BfaI | CTAG | 9 cut(s) | 417, 1091, 1628, 2013, 2057, 2457, 2526, 2907, 3491 |
| BfmI | CTRYAG | 4 cut(s) | 903, 1725, 1743, 2196 |
| BfoI | RGCGCY | 1 cut(s) | 3465 |
| BfuAI | ACCTGC | 3 cut(s) | 1100, 1246, 2961 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2259 |
| BglII | AGATCT | 1 cut(s) | 2405 |
| BlnI | CCTAGG | 2 cut(s) | 2056, 3490 |
| BmcAI | AGTACT | 1 cut(s) | 148 |
| Bme1390I | CCNGG | 8 cut(s) | 324, 480, 761, 1043, 1076, 2166, 2842, 2868 |
| Bme18I | GGWCC | 5 cut(s) | 113, 265, 1685, 2876, 2973 |
| BmeT110I | CYCGRG | 1 cut(s) | 3477 |
| BmgBI | CACGTC | 1 cut(s) | 2831 |
| BmgT120I | GGNCC | 7 cut(s) | 113, 265, 603, 1685, 2825, 2876, 2973 |
| BmiI | GGNNCC | 9 cut(s) | 132, 864, 973, 1668, 1687, 2506, 2568, 2975, 3525 |
| BmrFI | CCNGG | 8 cut(s) | 324, 480, 761, 1043, 1076, 2166, 2842, 2868 |
| BmrI | ACTGGG | 2 cut(s) | 838, 1268 |
| BmuI | ACTGGG | 2 cut(s) | 838, 1268 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1436 |
| BpiI | GAAGAC | 2 cut(s) | 217, 798 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 482, 514 |
| BpmI | CTGGAG | 1 cut(s) | 3153 |
| Bpu10I | CCTNAGC | 1 cut(s) | 3528 |
| BpuEI | CTTGAG | 1 cut(s) | 876 |
| BpuMI | CCSGG | 3 cut(s) | 1043, 2166, 2868 |
| BsaAI | YACGTR | 2 cut(s) | 341, 2417 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1785, 2491 |
| BsaI | GGTCTC | 1 cut(s) | 1629 |
| BsaJI | CCNNGG | 7 cut(s) | 86, 606, 1041, 2056, 2391, 2866, 3490 |
| BsaWI | WCCGGW | 1 cut(s) | 1420 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 3148, 3178 |
| Bse118I | RCCGGY | 1 cut(s) | 463 |
| Bse1I | ACTGG | 8 cut(s) | 781, 833, 944, 1242, 1263, 2104, 2239, 2902 |
| Bse3DI | GCAATG | 3 cut(s) | 1709, 2568, 3130 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1785, 2491 |
| BseAI | TCCGGA | 1 cut(s) | 1420 |
| BseBI | CCWGG | 5 cut(s) | 324, 480, 761, 1076, 2842 |
| BseDI | CCNNGG | 7 cut(s) | 86, 606, 1041, 2056, 2391, 2866, 3490 |
| BseJI | GATNNNNATC | 2 cut(s) | 1785, 2491 |
| BseMI | GCAATG | 3 cut(s) | 1709, 2568, 3130 |
| BseMII | CTCAG | 6 cut(s) | 314, 450, 1601, 2199, 2358, 3276 |
| BseNI | ACTGG | 8 cut(s) | 781, 833, 944, 1242, 1263, 2104, 2239, 2902 |
| BseRI | GAGGAG | 7 cut(s) | 875, 1901, 2347, 2384, 2752, 2916, 3174 |
| BseSI | GKGCMC | 3 cut(s) | 85, 1255, 3021 |
| BseYI | CCCAGC | 1 cut(s) | 1619 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1440 |
| BshFI | GGCC | 7 cut(s) | 604, 1074, 1539, 2253, 2520, 2788, 2827 |
| BshNI | GGYRCC | 1 cut(s) | 3523 |
| BsiEI | CGRYCG | 1 cut(s) | 1440 |
| BsiHKAI | GWGCWC | 3 cut(s) | 85, 1255, 2805 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3477 |
| BsiSI | CCGG | 5 cut(s) | 464, 1043, 1421, 2166, 2868 |
| BslFI | GGGAC | 3 cut(s) | 592, 1671, 2959 |
| BsmAI | GTCTC | 2 cut(s) | 1629, 2107 |
| BsmFI | GGGAC | 3 cut(s) | 592, 1671, 2959 |
| BsmI | GAATGC | 1 cut(s) | 1985 |
| BsnI | GGCC | 7 cut(s) | 604, 1074, 1539, 2253, 2520, 2788, 2827 |
| Bso31I | GGTCTC | 1 cut(s) | 1629 |
| BsoBI | CYCGRG | 1 cut(s) | 3477 |
| Bsp1286I | GDGCHC | 5 cut(s) | 85, 1255, 1382, 2805, 3021 |
| Bsp13I | TCCGGA | 1 cut(s) | 1420 |
| Bsp19I | CCATGG | 1 cut(s) | 2391 |
| BspACI | CCGC | 8 cut(s) | 128, 1436, 2001, 2521, 2591, 2773, 3358, 3424 |
| BspANI | GGCC | 7 cut(s) | 604, 1074, 1539, 2253, 2520, 2788, 2827 |
| BspCNI | CTCAG | 6 cut(s) | 313, 451, 1600, 2200, 2359, 3275 |
| BspEI | TCCGGA | 1 cut(s) | 1420 |
| BspLI | GGNNCC | 9 cut(s) | 132, 864, 973, 1668, 1687, 2506, 2568, 2975, 3525 |
| BspMAI | CTGCAG | 2 cut(s) | 1729, 2200 |
| BspMI | ACCTGC | 3 cut(s) | 1100, 1246, 2961 |
| BspT107I | GGYRCC | 1 cut(s) | 3523 |
| BspTNI | GGTCTC | 1 cut(s) | 1629 |
| BsrDI | GCAATG | 3 cut(s) | 1709, 2568, 3130 |
| BsrFI | RCCGGY | 1 cut(s) | 463 |
| BsrI | ACTGG | 8 cut(s) | 781, 833, 944, 1242, 1263, 2104, 2239, 2902 |
| BssAI | RCCGGY | 1 cut(s) | 463 |
| BssECI | CCNNGG | 7 cut(s) | 86, 606, 1041, 2056, 2391, 2866, 3490 |
| BssSI | CACGAG | 2 cut(s) | 570, 1697 |
| BssT1I | CCWWGG | 5 cut(s) | 86, 606, 2056, 2391, 3490 |
| Bst2BI | CACGAG | 2 cut(s) | 570, 1697 |
| Bst2UI | CCWGG | 5 cut(s) | 324, 480, 761, 1076, 2842 |
| Bst6I | CTCTTC | 3 cut(s) | 667, 1419, 1454 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1520, 2600 |
| BstBAI | YACGTR | 2 cut(s) | 341, 2417 |
| BstC8I | GCNNGC | 5 cut(s) | 135, 465, 529, 1198, 1512 |
| BstDEI | CTNAG | 9 cut(s) | 66, 300, 459, 984, 1587, 2208, 2367, 3262, 3528 |
| BstDSI | CCRYGG | 1 cut(s) | 2391 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 598, 1515, 2308 |
| BstH2I | RGCGCY | 1 cut(s) | 3465 |
| BstHHI | GCGC | 2 cut(s) | 2858, 3464 |
| BstMAI | GTCTC | 2 cut(s) | 1629, 2107 |
| BstMCI | CGRYCG | 1 cut(s) | 1440 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 134, 524, 537, 1085, 1520, 2250, 2259, 2600 |
| BstNI | CCWGG | 5 cut(s) | 324, 480, 761, 1076, 2842 |
| BstNSI | RCATGY | 3 cut(s) | 239, 1158, 1938 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1436 |
| BstSCI | CCNGG | 8 cut(s) | 322, 478, 759, 1041, 1074, 2164, 2840, 2866 |
| BstSFI | CTRYAG | 4 cut(s) | 903, 1725, 1743, 2196 |
| BstSLI | GKGCMC | 3 cut(s) | 85, 1255, 3021 |
| BstV2I | GAAGAC | 2 cut(s) | 217, 798 |
| BsuRI | GGCC | 7 cut(s) | 604, 1074, 1539, 2253, 2520, 2788, 2827 |
| BtgI | CCRYGG | 1 cut(s) | 2391 |
| BtrI | CACGTC | 1 cut(s) | 2831 |
| BtsI | GCAGTG | 1 cut(s) | 3310 |
| BtsIMutI | CAGTG | 8 cut(s) | 226, 417, 826, 1353, 1941, 3207, 3280, 3310 |
| BveI | ACCTGC | 3 cut(s) | 1100, 1246, 2961 |
| Cac8I | GCNNGC | 5 cut(s) | 135, 465, 529, 1198, 1512 |
| CfoI | GCGC | 2 cut(s) | 2858, 3464 |
| Cfr10I | RCCGGY | 1 cut(s) | 463 |
| Cfr13I | GGNCC | 7 cut(s) | 113, 265, 603, 1685, 2825, 2876, 2973 |
| CseI | GACGC | 3 cut(s) | 792, 971, 3374 |
| CsiI | ACCWGGT | 1 cut(s) | 322 |
| Csp6I | GTAC | 8 cut(s) | 14, 147, 203, 719, 1722, 1874, 2033, 3524 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2464, 2499 |
| CviQI | GTAC | 8 cut(s) | 14, 147, 203, 719, 1722, 1874, 2033, 3524 |
| DdeI | CTNAG | 9 cut(s) | 66, 300, 459, 984, 1587, 2208, 2367, 3262, 3528 |
| EaeI | YGGCCR | 1 cut(s) | 2518 |
| Eam1104I | CTCTTC | 3 cut(s) | 667, 1419, 1454 |
| EarI | CTCTTC | 3 cut(s) | 667, 1419, 1454 |
| Ecl136II | GAGCTC | 1 cut(s) | 2803 |
| Eco130I | CCWWGG | 5 cut(s) | 86, 606, 2056, 2391, 3490 |
| Eco147I | AGGCCT | 2 cut(s) | 1539, 2788 |
| Eco24I | GRGCYC | 2 cut(s) | 1382, 2805 |
| Eco31I | GGTCTC | 1 cut(s) | 1629 |
| Eco32I | GATATC | 2 cut(s) | 663, 2747 |
| Eco47I | GGWCC | 5 cut(s) | 113, 265, 1685, 2876, 2973 |
| Eco53kI | GAGCTC | 1 cut(s) | 2803 |
| Eco57I | CTGAAG | 5 cut(s) | 330, 393, 2196, 3348, 3393 |
| Eco88I | CYCGRG | 1 cut(s) | 3477 |
| EcoICRI | GAGCTC | 1 cut(s) | 2803 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 598, 1515, 2308 |
| EcoO109I | RGGNCCY | 3 cut(s) | 603, 2876, 2973 |
| EcoRII | CCWGG | 5 cut(s) | 322, 478, 759, 1074, 2840 |
| EcoRV | GATATC | 2 cut(s) | 663, 2747 |
| EcoT14I | CCWWGG | 5 cut(s) | 86, 606, 2056, 2391, 3490 |
| EcoT22I | ATGCAT | 3 cut(s) | 589, 682, 1156 |
| EcoT38I | GRGCYC | 2 cut(s) | 1382, 2805 |
| ErhI | CCWWGG | 5 cut(s) | 86, 606, 2056, 2391, 3490 |
| FaqI | GGGAC | 3 cut(s) | 592, 1671, 2959 |
| FauI | CCCGC | 3 cut(s) | 1994, 2766, 3431 |
| FblI | GTMKAC | 2 cut(s) | 906, 2890 |
| FriOI | GRGCYC | 2 cut(s) | 1382, 2805 |
| FspBI | CTAG | 9 cut(s) | 417, 1091, 1628, 2013, 2057, 2457, 2526, 2907, 3491 |
| GlaI | GCGC | 2 cut(s) | 2857, 3463 |
| GsaI | CCCAGC | 1 cut(s) | 1623 |
| GsuI | CTGGAG | 1 cut(s) | 3153 |
| HaeII | RGCGCY | 1 cut(s) | 3465 |
| HaeIII | GGCC | 7 cut(s) | 604, 1074, 1539, 2253, 2520, 2788, 2827 |
| HapII | CCGG | 5 cut(s) | 464, 1043, 1421, 2166, 2868 |
| HgaI | GACGC | 3 cut(s) | 792, 971, 3374 |
| HhaI | GCGC | 2 cut(s) | 2858, 3464 |
| Hin6I | GCGC | 2 cut(s) | 2856, 3462 |
| HinP1I | GCGC | 2 cut(s) | 2856, 3462 |
| HincII | GTYRAC | 1 cut(s) | 1765 |
| HindII | GTYRAC | 1 cut(s) | 1765 |
| HindIII | AAGCTT | 2 cut(s) | 188, 2178 |
| HpaII | CCGG | 5 cut(s) | 464, 1043, 1421, 2166, 2868 |
| Hpy166II | GTNNAC | 7 cut(s) | 83, 907, 1253, 1765, 2611, 2891, 3487 |
| Hpy8I | GTNNAC | 7 cut(s) | 83, 907, 1253, 1765, 2611, 2891, 3487 |
| Hpy99I | CGWCG | 4 cut(s) | 2484, 2835, 3084, 3474 |
| HpyCH4IV | ACGT | 7 cut(s) | 340, 1912, 2031, 2416, 2479, 2830, 3079 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 134, 524, 537, 1085, 1520, 2250, 2259, 2600 |
| HpyF3I | CTNAG | 9 cut(s) | 66, 300, 459, 984, 1587, 2208, 2367, 3262, 3528 |
| HpySE526I | ACGT | 7 cut(s) | 340, 1912, 2031, 2416, 2479, 2830, 3079 |
| HspAI | GCGC | 2 cut(s) | 2856, 3462 |
| Kpn2I | TCCGGA | 1 cut(s) | 1420 |
| KpnI | GGTACC | 1 cut(s) | 3527 |
| KroI | GCCGGC | 1 cut(s) | 463 |
| KroNI | GCCGGC | 1 cut(s) | 465 |
| LmnI | GCTCC | 5 cut(s) | 130, 862, 2201, 2318, 3041 |
| MabI | ACCWGGT | 1 cut(s) | 322 |
| MaeI | CTAG | 9 cut(s) | 417, 1091, 1628, 2013, 2057, 2457, 2526, 2907, 3491 |
| MaeII | ACGT | 7 cut(s) | 340, 1912, 2031, 2416, 2479, 2830, 3079 |
| MfeI | CAATTG | 2 cut(s) | 372, 2159 |
| MhlI | GDGCHC | 5 cut(s) | 85, 1255, 1382, 2805, 3021 |
| MlyI | GAGTC | 3 cut(s) | 1040, 2336, 3469 |
| MmeI | TCCRAC | 3 cut(s) | 35, 1807, 3202 |
| Mph1103I | ATGCAT | 3 cut(s) | 589, 682, 1156 |
| MroI | TCCGGA | 1 cut(s) | 1420 |
| MroNI | GCCGGC | 1 cut(s) | 463 |
| MroXI | GAANNNNTTC | 2 cut(s) | 693, 999 |
| MseI | TTAA | 8 cut(s) | 246, 455, 765, 1506, 1556, 1602, 2022, 2256 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1713, 1776, 2024, 2235, 2616 |
| MspA1I | CMGCKG | 2 cut(s) | 1436, 1676 |
| MspI | CCGG | 5 cut(s) | 464, 1043, 1421, 2166, 2868 |
| MspR9I | CCNGG | 8 cut(s) | 324, 480, 761, 1043, 1076, 2166, 2842, 2868 |
| MunI | CAATTG | 2 cut(s) | 372, 2159 |
| Mva1269I | GAATGC | 1 cut(s) | 1985 |
| MvaI | CCWGG | 5 cut(s) | 324, 480, 761, 1076, 2842 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 134, 524, 537, 1085, 1520, 2250, 2259, 2600 |
| NaeI | GCCGGC | 1 cut(s) | 465 |
| NciI | CCSGG | 3 cut(s) | 1043, 2166, 2868 |
| NcoI | CCATGG | 1 cut(s) | 2391 |
| NgoMIV | GCCGGC | 1 cut(s) | 463 |
| NlaIV | GGNNCC | 9 cut(s) | 132, 864, 973, 1668, 1687, 2506, 2568, 2975, 3525 |
| NsiI | ATGCAT | 3 cut(s) | 589, 682, 1156 |
| NspI | RCATGY | 3 cut(s) | 239, 1158, 1938 |
| OliI | CACNNNNGTG | 2 cut(s) | 1713, 2616 |
| PaeR7I | CTCGAG | 1 cut(s) | 3477 |
| PaqCI | CACCTGC | 2 cut(s) | 1100, 1246 |
| PceI | AGGCCT | 2 cut(s) | 1539, 2788 |
| PciI | ACATGT | 1 cut(s) | 1934 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 2805, 3358, 3475 |
| PctI | GAATGC | 1 cut(s) | 1985 |
| PdiI | GCCGGC | 1 cut(s) | 465 |
| PdmI | GAANNNNTTC | 2 cut(s) | 693, 999 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2537 |
| PleI | GAGTC | 3 cut(s) | 1040, 2336, 3469 |
| PpsI | GAGTC | 3 cut(s) | 1040, 2336, 3469 |
| Ppu21I | YACGTR | 2 cut(s) | 341, 2417 |
| PpuMI | RGGWCCY | 2 cut(s) | 2876, 2973 |
| PscI | ACATGT | 1 cut(s) | 1934 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1436 |
| PsiI | TTATAA | 1 cut(s) | 2643 |
| Psp124BI | GAGCTC | 1 cut(s) | 2805 |
| Psp5II | RGGWCCY | 2 cut(s) | 2876, 2973 |
| Psp6I | CCWGG | 5 cut(s) | 322, 478, 759, 1074, 2840 |
| PspFI | CCCAGC | 1 cut(s) | 1619 |
| PspGI | CCWGG | 5 cut(s) | 322, 478, 759, 1074, 2840 |
| PspN4I | GGNNCC | 9 cut(s) | 132, 864, 973, 1668, 1687, 2506, 2568, 2975, 3525 |
| PspPI | GGNCC | 7 cut(s) | 113, 265, 603, 1685, 2825, 2876, 2973 |
| PspPPI | RGGWCCY | 2 cut(s) | 2876, 2973 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 139, 171 |
| PstI | CTGCAG | 2 cut(s) | 1729, 2200 |
| PvuII | CAGCTG | 1 cut(s) | 1676 |
| RsaI | GTAC | 8 cut(s) | 15, 148, 204, 720, 1723, 1875, 2034, 3525 |
| RsaNI | GTAC | 8 cut(s) | 14, 147, 203, 719, 1722, 1874, 2033, 3524 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1713, 1776, 2024, 2235, 2616 |
| SacI | GAGCTC | 1 cut(s) | 2805 |
| SaqAI | TTAA | 8 cut(s) | 246, 455, 765, 1506, 1556, 1602, 2022, 2256 |
| Sau96I | GGNCC | 7 cut(s) | 113, 265, 603, 1685, 2825, 2876, 2973 |
| ScaI | AGTACT | 1 cut(s) | 148 |
| SchI | GAGTC | 3 cut(s) | 1040, 2336, 3469 |
| ScrFI | CCNGG | 8 cut(s) | 324, 480, 761, 1043, 1076, 2166, 2842, 2868 |
| SduI | GDGCHC | 5 cut(s) | 85, 1255, 1382, 2805, 3021 |
| SexAI | ACCWGGT | 1 cut(s) | 322 |
| SfcI | CTRYAG | 4 cut(s) | 903, 1725, 1743, 2196 |
| Sfr274I | CTCGAG | 1 cut(s) | 3477 |
| SinI | GGWCC | 5 cut(s) | 113, 265, 1685, 2876, 2973 |
| SlaI | CTCGAG | 1 cut(s) | 3477 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1713, 1776, 2024, 2235, 2616 |
| SmlI | CTYRAG | 2 cut(s) | 891, 3477 |
| SmoI | CTYRAG | 2 cut(s) | 891, 3477 |
| SpeI | ACTAGT | 1 cut(s) | 416 |
| SseBI | AGGCCT | 2 cut(s) | 1539, 2788 |
| SsiI | CCGC | 8 cut(s) | 128, 1436, 2001, 2521, 2591, 2773, 3358, 3424 |
| SspI | AATATT | 1 cut(s) | 1192 |
| SspMI | CTAG | 9 cut(s) | 417, 1091, 1628, 2013, 2057, 2457, 2526, 2907, 3491 |
| SstI | GAGCTC | 1 cut(s) | 2805 |
| StuI | AGGCCT | 2 cut(s) | 1539, 2788 |
| StyD4I | CCNGG | 8 cut(s) | 322, 478, 759, 1041, 1074, 2164, 2840, 2866 |
| StyI | CCWWGG | 5 cut(s) | 86, 606, 2056, 2391, 3490 |
| TaiI | ACGT | 7 cut(s) | 343, 1915, 2034, 2419, 2482, 2833, 3082 |
| TaqI | TCGA | 7 cut(s) | 1293, 1440, 2544, 2920, 3297, 3469, 3478 |
| TatI | WGTACW | 3 cut(s) | 146, 202, 718 |
| TauI | GCSGC | 3 cut(s) | 2523, 2594, 3361 |
| Tru1I | TTAA | 8 cut(s) | 246, 455, 765, 1506, 1556, 1602, 2022, 2256 |
| Tru9I | TTAA | 8 cut(s) | 246, 455, 765, 1506, 1556, 1602, 2022, 2256 |
| TscAI | CASTG | 8 cut(s) | 226, 417, 833, 1360, 1948, 3207, 3280, 3310 |
| TspGWI | ACGGA | 7 cut(s) | 257, 264, 964, 1829, 2956, 3383, 3487 |
| TspRI | CASTG | 8 cut(s) | 226, 417, 833, 1360, 1948, 3207, 3280, 3310 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2537 |
| VneI | GTGCAC | 2 cut(s) | 81, 1251 |
| VpaK11BI | GGWCC | 5 cut(s) | 113, 265, 1685, 2876, 2973 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 598, 1515, 2308 |
| XapI | RAATTY | 3 cut(s) | 31, 1895, 1994 |
| XbaI | TCTAGA | 2 cut(s) | 1627, 2906 |
| XceI | RCATGY | 3 cut(s) | 239, 1158, 1938 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 51, 1715 |
| XhoI | CTCGAG | 1 cut(s) | 3477 |
| XmaJI | CCTAGG | 2 cut(s) | 2056, 3490 |
| XmiI | GTMKAC | 2 cut(s) | 906, 2890 |
| XmnI | GAANNNNTTC | 2 cut(s) | 693, 999 |
| XspI | CTAG | 9 cut(s) | 417, 1091, 1628, 2013, 2057, 2457, 2526, 2907, 3491 |
| ZrmI | AGTACT | 1 cut(s) | 148 |
| Zsp2I | ATGCAT | 3 cut(s) | 589, 682, 1156 |