MD07G1277900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001112
Physical Location & Seq
Forward (+)
34290066 .. 34296395
6330 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1277900.v1.1.491

Sequence Viewer

Length: 3642 bp
ATGATGAATACAGTACGTGTAGAACTTACAAAATCTATTAGTCCTTACCTGAACTGGAAGGTTTCAAAGGGTTATCGGAGTGCATCAAGGCGGTCACGGAAACCTGTTGATAAAAGCAAAAAAATGGGTGCAGACCTAGCAGAAAAAAGTGCTAGAAGTGCATCAGAAACATCAGTTTCTGATTCAGAGAAGCTTGGTGTGGCTGTACTTGGGAGACGATTTGGTGACAAAATTGAGCATGTACCGATTAAGAAAAGGAGACTTATGGTCCGGTCTCCGTCACCCCCTCCACACCTTGAAGGCAACAAACCACTATTGGATGGTCGTCATTCTTCAGGTCACAAGTCTTGTCCAAAATCAGTTGGCAAAAAGCATCCCACAAGGAGTGATACTTCTACCCTGACTAGGGTCTGTCATAGTGCCAGTAGCGGTGCTAATGAGAATTTGAACGAGATGACAAATCAAAAGCCTGGTGACGTGGATGACTTTTCAGGTATTGAGATATTGGCAGATGCTGCCTGCAACAACATCATAAATGATGATATCAATCATGTTGTGAAGAATCCACTAGGTGAAGATTCACCTCTGGATGAAAAAGATGCATCAACTTCTGCATGGCCCTTGGAGCAAACAACCATTGTGTCAACTTCTTTAACAGTCATGAGAACTGCTTCAGAATTTTCAGAAGCAAGAGATGCATCAGTCTCTGCAATCTTGGAAGAAAGTATTGCATCATTGGAAACAGTACATTCATCCCCTAAAGATGTACGACGAGAAGATAAAATCGGGAGCGCTTCCTTTGAAGCTGATGGGTTCAGAATTAATACTGACAAAGCACACGACGAAGCAGAAGCAAGATTGAGTTCCTCAAAAGATGTCAAGTTCCACTGGGACCTAAATGTTAGTATGGATGCTTGGGAGGAACCTTGTGATTTGGCGATTGCTGATCCTAAAACAACTGCTGCAGATGATATCTCCATGGATAATAAGCAGGGTGCAAACGTCCAAGTTTCAGAAGCTAATGAGATACCTAAGGAGGAAGATGCAAAGAATGACATTGCAAGCACCGTGAAACCTCTGTCTGACAATGAGGAACAGGGATTGAAAGCGTGCCCTGAAGTTGACTTGAGTTGTGGTAAATGTGTTTCTCCTGATAATGCTTTGGGATCTTCAAACGATTCTGGTAGCAGTGCTAAAGCTTCCTCTCAGGATGCGTGTGTAGATGCATGCATTGATGGTTCCCCTTGTGCTGGACTTGAAAACATCGCAGTCACAGGCCTTGTGTCTGAGGAGACCGATAAAACCCCAATTTCCAGCTTTTCTGTTGAGCATGTGGCTGGTGAACTACCAAGTGAAACTGTCTGTTCAGGAAGTGTTAAGGTTGAAAACCCTGCTTTAGCTTGTGTGCCAGAAGGGGCATCTTGTGAGATTGAAAGCACTGTTCTAGATGAAGATGGCAAGGGCTCTGGTGCAACATCTAGCGTCCATGATGATCCAGAATCTCTGGAAGAGATGATGGGAGTAGAAAGCTGTCACTCGCTCGCACCCGTTCGCCTTGATTCTGATGAAAAATCTGCATCAGGTGCTTCTGTTGGAGAAGGCCGGTCGCTGGTGACTATAATTGCAAAGGAGCCTGTTGAAGCTGCCTCTGACACTCATACTGTCAATTCTCTGCCAAATGATGGTTCTGAGGAAGTAATGCATAAACCTGCAGGGAATCCCATGACAAATCAAGTAGCTGCTGCTGGGTCCTCCTCTCTTGAGCGATGCCACTATGGCGAAGATAGCTCACGTAGCTTGGATAGAGTAACTAAAGATCCTTCCAGTGATGGTTATGACTTGAATACTTGTCAAGATGATAAGGATCACATGGTTGGCAAGGGAAACACTCTGGAACCTGAAGCTGGTTACGACTCCCAGTATGAAGATGGGGAGCTGAGGGAGTCGGATGTACCATATTGGGAGGAGAATGAAATTGATGACGTAGAAGCTGAATGTGTTGACTATGGTTCTGATACATGTGACAGTGAAGCTGCTGATGACTCTATATCAGGGAAAGTTGGAATGGGACCTGAATGCAGAGAAACAGAATTATTTGGGGAGTCAAGGAAAATTGACAGGAACATTAAGCTTGTGAGAGGAGTGAGTCCAGGATCTGATAACATGTGTGACAAAAATGAACATGCTTTGAGACAATGTTCCGTTGGTTCGAAGACAAAAACTTCAGGATCTGATCAACTACCTTGTGATTCTGAAGCTTCTTCAAACAGAACTGCGGAAGCAATTGAGGGCTGCACAGTAAGGAGACATGCTGTTAATGGCTTCGATGGCTATGATGCCAAACATTCTCCTGCCAACGTGGTTGGATCAATGGCATCTGACTCCTCAAATAAGATGGGTACTGAATGTGCAAGGAGGAGGAGGTTGGGCAACTTTGATTCTATTCGCTCTGAGGAAGCTGGTTCTGATCAGACCATGGGAAGGGAAAAGTATGACTCACATATGCAGGGTAAAAGCTTCAGAGGTGTGGTTTATTCTTCAGGGAGTTATTGGGATTCCAAGCGACGCGAATCACCCACTTATCATGGTTCCTTTGGCTCTGGACGCTGTAGGCCAAGAAGTGTTGTTGAGAACCATGGATATGAGATGGAATCAGACGAAACATTTTCAGAAGCACCAGGAGTTCACAACCGTGTCCGCAGGCAAGCTATAACTTTTTCATCAAATCGTCCGTATCAGGGTTCGTTTAGGAGAAGATCGCCATCTGAAAGAAATGATGCACACAACATACACAGGGGAATGATACCTATGAGAGATACAAGTCCTGATAGGCGGAGGTTTAGACGATATCCACAAGGGGTTAACAGAGGCATCAGGGAGGACTACCACAGATCCATACCCGATGATCCCAACGAATGCTCTTATAATGTGCCACGACGAACGGCCAGGAGAGAACAAAGCACTTCACCCCCTGGCAGAGGACCCATATATTATAGTAGACCCTACAAAAAGCCCCAGTCTAGATGCAGAAGTCGCTCTCCTCTTGGATGGAGAGAACTGAATGATATTTCAAGACATCGTGGGAGCAGGTCTCCTGATGATAGGTTTGATTCCAATATCGAGAAGCTGAGGGTGCCTTTCCAGAAGCAAAATTTCGGAGGCAAGTATGAAGTAGGGTTCGTGTCCTCACCAAAGCGTCGTTTTTCCCCACAACAGAATTCCAGATGGTTTGATGATTCAAATACAGGTGTAGATCACAATTTCAGGGGCAGGAGATTTGCGGGAAGGCGGTTCCAGCCAGGACAGAGGTTTGACTCAGAGCGTTCATCTCAGAGATTGAATTCAGATGGTTACTCTGAATCCATGATTCGTCCAGCAAAATTCTCCGAGTTACCCAGTGGTGGTAGGGAGTATAGATACGAGGGTAGCGACGATGATAGACGGAGACCTGATGGAAGATTTGAGATCGTTCGCCGCGTGAGGCGTTTTGATTCAAATGGTGTTGTGCGTCAGTTCCGCTTTGATGAAGACCGTTTTGCATCTCGCAACACACACAACTACGATGAATCTGACAACAGGGCAACCGAGAGAAGGCCTCGAGAAGCCTACGTAGGAGAAGTAGCGAAGAGGAGAGTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1214

Amino Acids

133.89

Weight (kDa)

5.62

Isoelectric Point (pI)

66.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2925
Acc36I ACCTGC 2 cut(s) 1717, 3078
AccB1I GGYRCC 1 cut(s) 3133
AccB7I CCANNNNNTGG 1 cut(s) 1682
AccI GTMKAC 1 cut(s) 2998
AccII CGCG 2 cut(s) 2568, 3477
AciI CCGC 9 cut(s) 91, 429, 2276, 2698, 2833, 3281, 3289, 3475, 3517
AcoI YGGCCR 1 cut(s) 2943
AcsI RAATTY 6 cut(s) 442, 677, 3151, 3217, 3340, 3380
AcuI CTGAAG 8 cut(s) 318, 657, 1137, 1920, 2208, 2274, 2503, 2523
AdeI CACNNNGTG 1 cut(s) 572
AfaI GTAC 7 cut(s) 15, 207, 243, 747, 768, 1953, 2401
AfeI AGCGCT 1 cut(s) 793
AflIII ACRYGT 3 cut(s) 16, 2018, 2163
AhdI GACNNNNNGTC 1 cut(s) 266
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 6 cut(s) 469, 2149, 2677, 2945, 2971, 3298
AjuI GAANNNNNNNTTGG 2 cut(s) 1669, 1701
AleI CACNNNNGTG 1 cut(s) 2691
Alw26I GTCTC 9 cut(s) 208, 253, 279, 709, 1286, 2185, 2299, 3096, 3439
AlwNI CAGNNNCTG 5 cut(s) 179, 515, 707, 2156, 2231
Ama87I CYCGRG 1 cut(s) 3597
Aor51HI AGCGCT 1 cut(s) 793
AoxI GGCC 6 cut(s) 617, 1276, 1600, 2612, 2943, 3593
ApeKI GCWGC 7 cut(s) 515, 962, 1643, 1739, 1742, 2033, 2292
ApoI RAATTY 6 cut(s) 442, 677, 3151, 3217, 3340, 3380
AseI ATTAAT 1 cut(s) 822
Asp700I GAANNNNTTC 1 cut(s) 670
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 6 cut(s) 268, 618, 892, 1749, 2069, 2981
AsuII TTCGAA 1 cut(s) 2210
AvaI CYCGRG 1 cut(s) 3597
AvaII GGWCC 5 cut(s) 268, 892, 1749, 2069, 2981
AxyI CCTNAGG 1 cut(s) 1032
BaeGI GKGCMC 1 cut(s) 1115
BaeI ACNNNNGTAYC 2 cut(s) 3409, 3442
BanI GGYRCC 1 cut(s) 3133
BanII GRGCYC 1 cut(s) 1466
BbsI GAAGAC 2 cut(s) 2219, 3534
BbvCI CCTCAGC 2 cut(s) 1937, 3128
BbvI GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2279
BceAI ACGGC 1 cut(s) 2958
BciT130I CCWGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
BclI TGATCA 2 cut(s) 2233, 2467
BcoDI GTCTC 9 cut(s) 208, 253, 279, 709, 1286, 2185, 2299, 3096, 3439
BfaI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 3021
BfmI CTRYAG 3 cut(s) 963, 1710, 2608
BfoI RGCGCY 1 cut(s) 795
BfuAI ACCTGC 2 cut(s) 1717, 3078
BglI GCCNNNNNGGC 1 cut(s) 1776
BisI GCNGC 8 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2293, 3475
BlsI GCNGC 8 cut(s) 517, 964, 1645, 1741, 1744, 2035, 2294, 3476
Bme1390I CCNGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
Bme18I GGWCC 5 cut(s) 268, 892, 1749, 2069, 2981
BmeRI GACNNNNNGTC 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 3597
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 6 cut(s) 268, 618, 892, 1749, 2069, 2981
BmrFI CCNGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
BmrI ACTGGG 4 cut(s) 898, 1912, 3010, 3390
BmuI ACTGGG 4 cut(s) 898, 1912, 3010, 3390
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 2 cut(s) 2219, 3534
BplI GAGNNNNNCTC 4 cut(s) 3076, 3108, 3580, 3612
Bpu10I CCTNAGC 2 cut(s) 1937, 3128
Bpu14I TTCGAA 1 cut(s) 2210
BpuEI CTTGAG 2 cut(s) 1147, 1781
BsaAI YACGTR 3 cut(s) 17, 1793, 3610
BsaBI GATNNNNATC 3 cut(s) 546, 1863, 2761
BsaI GGTCTC 4 cut(s) 279, 1286, 3096, 3439
BsaJI CCNNGG 5 cut(s) 621, 978, 2475, 2635, 2971
BsaWI WCCGGW 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 1602
Bse1I ACTGG 7 cut(s) 59, 423, 893, 1824, 1918, 3016, 3396
Bse21I CCTNAGG 1 cut(s) 1032
Bse3DI GCAATG 1 cut(s) 1056
Bse8I GATNNNNATC 3 cut(s) 546, 1863, 2761
BseBI CCWGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
BseDI CCNNGG 5 cut(s) 621, 978, 2475, 2635, 2971
BseGI GGATG 9 cut(s) 325, 373, 487, 595, 752, 916, 1216, 1954, 3053
BseJI GATNNNNATC 3 cut(s) 546, 1863, 2761
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 8 cut(s) 1220, 1278, 1680, 1928, 2442, 3119, 3330, 3344
BseNI ACTGG 7 cut(s) 59, 423, 893, 1824, 1918, 3016, 3396
BseRI GAGGAG 8 cut(s) 1304, 1744, 1979, 2154, 2374, 2431, 2434, 3030
BseSI GKGCMC 1 cut(s) 1115
BseXI GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2279
BseYI CCCAGC 1 cut(s) 1745
BsgI GTGCAG 2 cut(s) 150, 2278
Bsh1236I CGCG 2 cut(s) 2568, 3477
Bsh1285I CGRYCG 1 cut(s) 1607
BshFI GGCC 6 cut(s) 619, 1278, 1602, 2614, 2945, 3595
BshNI GGYRCC 1 cut(s) 3133
BsiEI CGRYCG 1 cut(s) 1607
BsiHKCI CYCGRG 1 cut(s) 3597
BsiSI CCGG 2 cut(s) 271, 1603
BslFI GGGAC 2 cut(s) 905, 2082
BsmAI GTCTC 9 cut(s) 208, 253, 279, 709, 1286, 2185, 2299, 3096, 3439
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 2 cut(s) 905, 2082
BsmI GAATGC 2 cut(s) 2081, 2921
BsnI GGCC 6 cut(s) 619, 1278, 1602, 2614, 2945, 3595
Bso31I GGTCTC 4 cut(s) 279, 1286, 3096, 3439
BsoBI CYCGRG 1 cut(s) 3597
Bsp119I TTCGAA 1 cut(s) 2210
Bsp1286I GDGCHC 2 cut(s) 1115, 1466
Bsp19I CCATGG 3 cut(s) 978, 2475, 2635
BspACI CCGC 9 cut(s) 91, 429, 2276, 2698, 2833, 3281, 3289, 3475, 3517
BspANI GGCC 6 cut(s) 619, 1278, 1602, 2614, 2945, 3595
BspCNI CTCAG 8 cut(s) 1219, 1279, 1681, 1929, 2443, 3120, 3329, 3343
BspFNI CGCG 2 cut(s) 2568, 3477
BspHI TCATGA 1 cut(s) 660
BspMAI CTGCAG 2 cut(s) 967, 1714
BspMI ACCTGC 2 cut(s) 1717, 3078
BspT104I TTCGAA 1 cut(s) 2210
BspT107I GGYRCC 1 cut(s) 3133
BspTNI GGTCTC 4 cut(s) 279, 1286, 3096, 3439
BsrDI GCAATG 1 cut(s) 1056
BsrFI RCCGGY 1 cut(s) 1602
BsrI ACTGG 7 cut(s) 59, 423, 893, 1824, 1918, 3016, 3396
BssAI RCCGGY 1 cut(s) 1602
BssECI CCNNGG 5 cut(s) 621, 978, 2475, 2635, 2971
BssT1I CCWWGG 4 cut(s) 621, 978, 2475, 2635
Bst2UI CCWGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
Bst6I CTCTTC 2 cut(s) 1503, 3620
BstAPI GCANNNNNTGC 3 cut(s) 515, 695, 1583
BstBAI YACGTR 3 cut(s) 17, 1793, 3610
BstBI TTCGAA 1 cut(s) 2210
BstC8I GCNNGC 7 cut(s) 520, 1063, 1111, 1228, 1542, 2702, 2706
BstDEI CTNAG 9 cut(s) 1032, 1206, 1287, 1689, 1937, 2451, 3128, 3316, 3330
BstDSI CCRYGG 3 cut(s) 978, 2475, 2635
BstENI CCTNNNNNAGG 1 cut(s) 2976
BstF5I GGATG 9 cut(s) 325, 373, 487, 595, 752, 916, 1216, 1954, 3053
BstFNI CGCG 2 cut(s) 2568, 3477
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 1 cut(s) 794
BstMAI GTCTC 9 cut(s) 208, 253, 279, 709, 1286, 2185, 2299, 3096, 3439
BstMCI CGRYCG 1 cut(s) 1607
BstNI CCWGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
BstNSI RCATGY 7 cut(s) 242, 1230, 1334, 2022, 2167, 2186, 2312
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 6 cut(s) 469, 2149, 2677, 2945, 2971, 3298
BstSFI CTRYAG 3 cut(s) 963, 1710, 2608
BstSLI GKGCMC 1 cut(s) 1115
BstSNI TACGTA 1 cut(s) 3610
BstUI CGCG 2 cut(s) 2568, 3477
BstV1I GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2279
BstV2I GAAGAC 2 cut(s) 2219, 3534
BstX2I RGATCY 5 cut(s) 1166, 1816, 2153, 2228, 2891
BstYI RGATCY 5 cut(s) 1166, 1816, 2153, 2228, 2891
Bsu36I CCTNAGG 1 cut(s) 1032
BsuRI GGCC 6 cut(s) 619, 1278, 1602, 2614, 2945, 3595
BtgI CCRYGG 3 cut(s) 978, 2475, 2635
BtgZI GCGATG 2 cut(s) 1249, 1780
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 9 cut(s) 325, 373, 487, 595, 752, 916, 1216, 1954, 3053
BtsI GCAGTG 1 cut(s) 1195
BtsIMutI CAGTG 6 cut(s) 886, 1195, 1437, 1831, 2032, 3403
BveI ACCTGC 2 cut(s) 1717, 3078
Cac8I GCNNGC 7 cut(s) 520, 1063, 1111, 1228, 1542, 2702, 2706
CaiI CAGNNNCTG 5 cut(s) 179, 515, 707, 2156, 2231
CciI TCATGA 1 cut(s) 660
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 1 cut(s) 1602
Cfr13I GGNCC 6 cut(s) 268, 618, 892, 1749, 2069, 2981
CseI GACGC 5 cut(s) 1471, 2574, 2613, 3185, 3497
Csp6I GTAC 7 cut(s) 14, 206, 242, 746, 767, 1952, 2400
CviQI GTAC 7 cut(s) 14, 206, 242, 746, 767, 1952, 2400
DdeI CTNAG 9 cut(s) 1032, 1206, 1287, 1689, 1937, 2451, 3128, 3316, 3330
DraIII CACNNNGTG 1 cut(s) 572
DriI GACNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 1 cut(s) 2943
Eam1104I CTCTTC 2 cut(s) 1503, 3620
Eam1105I GACNNNNNGTC 1 cut(s) 266
EarI CTCTTC 2 cut(s) 1503, 3620
EciI GGCGGA 1 cut(s) 2848
Eco105I TACGTA 1 cut(s) 3610
Eco130I CCWWGG 4 cut(s) 621, 978, 2475, 2635
Eco147I AGGCCT 2 cut(s) 1278, 3595
Eco24I GRGCYC 1 cut(s) 1466
Eco31I GGTCTC 4 cut(s) 279, 1286, 3096, 3439
Eco32I GATATC 3 cut(s) 544, 973, 2849
Eco47I GGWCC 5 cut(s) 268, 892, 1749, 2069, 2981
Eco47III AGCGCT 1 cut(s) 793
Eco57I CTGAAG 8 cut(s) 318, 657, 1137, 1920, 2208, 2274, 2503, 2523
Eco81I CCTNAGG 1 cut(s) 1032
Eco88I CYCGRG 1 cut(s) 3597
EcoNI CCTNNNNNAGG 1 cut(s) 2976
EcoO109I RGGNCCY 4 cut(s) 892, 1749, 2069, 2981
EcoRI GAATTC 2 cut(s) 3217, 3340
EcoRII CCWGG 6 cut(s) 469, 2149, 2677, 2945, 2971, 3298
EcoRV GATATC 3 cut(s) 544, 973, 2849
EcoT14I CCWWGG 4 cut(s) 621, 978, 2475, 2635
EcoT22I ATGCAT 5 cut(s) 604, 700, 1228, 1232, 1704
EcoT38I GRGCYC 1 cut(s) 1466
ErhI CCWWGG 4 cut(s) 621, 978, 2475, 2635
Esp3I CGTCTC 1 cut(s) 208
FaqI GGGAC 2 cut(s) 905, 2082
FauI CCCGC 1 cut(s) 3274
FauNDI CATATG 1 cut(s) 2502
FbaI TGATCA 2 cut(s) 2233, 2467
FblI GTMKAC 1 cut(s) 2998
Fnu4HI GCNGC 8 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2293, 3475
FokI GGATG 9 cut(s) 332, 360, 494, 602, 739, 923, 1223, 1961, 3060
FriOI GRGCYC 1 cut(s) 1466
Fsp4HI GCNGC 8 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2293, 3475
FspBI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 3021
GlaI GCGC 1 cut(s) 793
GluI GCNGC 8 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2293, 3475
GsaI CCCAGC 1 cut(s) 1749
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 6 cut(s) 619, 1278, 1602, 2614, 2945, 3595
HapII CCGG 2 cut(s) 271, 1603
HgaI GACGC 5 cut(s) 1471, 2574, 2613, 3185, 3497
HhaI GCGC 1 cut(s) 794
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HincII GTYRAC 4 cut(s) 645, 1123, 2002, 2863
HindII GTYRAC 4 cut(s) 645, 1123, 2002, 2863
HindIII AAGCTT 5 cut(s) 191, 1197, 2129, 2256, 2515
HpaI GTTAAC 1 cut(s) 2863
HpaII CCGG 2 cut(s) 271, 1603
Hpy166II GTNNAC 7 cut(s) 645, 1123, 1343, 2002, 2686, 2863, 2999
Hpy8I GTNNAC 7 cut(s) 645, 1123, 1343, 2002, 2686, 2863, 2999
Hpy99I CGWCG 6 cut(s) 774, 845, 2568, 2940, 3201, 3434
HpyAV CCTTC 8 cut(s) 52, 293, 1406, 1592, 1830, 2475, 3279, 3585
HpyCH4IV ACGT 7 cut(s) 16, 477, 1002, 1792, 1983, 2358, 3609
HpyF3I CTNAG 9 cut(s) 1032, 1206, 1287, 1689, 1937, 2451, 3128, 3316, 3330
HpySE526I ACGT 7 cut(s) 16, 477, 1002, 1792, 1983, 2358, 3609
HspAI GCGC 1 cut(s) 792
Ksp22I TGATCA 2 cut(s) 2233, 2467
KspAI GTTAAC 1 cut(s) 2863
LmnI GCTCC 5 cut(s) 625, 789, 1630, 1933, 3084
Lsp1109I GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2279
MaeI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 3021
MaeII ACGT 7 cut(s) 16, 477, 1002, 1792, 1983, 2358, 3609
MfeI CAATTG 1 cut(s) 2283
MflI RGATCY 5 cut(s) 1166, 1816, 2153, 2228, 2891
MhlI GDGCHC 2 cut(s) 1115, 1466
MlyI GAGTC 8 cut(s) 1907, 1952, 2036, 2111, 2155, 2375, 2489, 3308
MmeI TCCRAC 4 cut(s) 1573, 1926, 2041, 2344
Mph1103I ATGCAT 5 cut(s) 604, 700, 1228, 1232, 1704
MroXI GAANNNNTTC 1 cut(s) 670
MseI TTAA 8 cut(s) 249, 653, 822, 1377, 2127, 2316, 2862, 3640
MslI CAYNNNNRTG 2 cut(s) 2640, 2691
MspI CCGG 2 cut(s) 271, 1603
MspR9I CCNGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
MunI CAATTG 1 cut(s) 2283
Mva1269I GAATGC 2 cut(s) 2081, 2921
MvaI CCWGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
MvnI CGCG 2 cut(s) 2568, 3477
NcoI CCATGG 3 cut(s) 978, 2475, 2635
NdeI CATATG 1 cut(s) 2502
NsiI ATGCAT 5 cut(s) 604, 700, 1228, 1232, 1704
NspI RCATGY 7 cut(s) 242, 1230, 1334, 2022, 2167, 2186, 2312
NspV TTCGAA 1 cut(s) 2210
OliI CACNNNNGTG 1 cut(s) 2691
PaeI GCATGC 1 cut(s) 1230
PaeR7I CTCGAG 1 cut(s) 3597
PagI TCATGA 1 cut(s) 660
PceI AGGCCT 2 cut(s) 1278, 3595
PciI ACATGT 2 cut(s) 2018, 2163
PcsI WCGNNNNNNNCGW 2 cut(s) 3426, 3474
PctI GAATGC 2 cut(s) 2081, 2921
PdmI GAANNNNTTC 1 cut(s) 670
PflMI CCANNNNNTGG 1 cut(s) 1682
PfoI TCCNGGA 1 cut(s) 2149
PkrI GCNGC 8 cut(s) 517, 964, 1645, 1741, 1744, 2035, 2294, 3476
PleI GAGTC 8 cut(s) 1907, 1951, 2036, 2110, 2154, 2375, 2489, 3308
PpsI GAGTC 8 cut(s) 1907, 1951, 2036, 2110, 2154, 2375, 2489, 3308
Ppu21I YACGTR 3 cut(s) 17, 1793, 3610
PpuMI RGGWCCY 4 cut(s) 892, 1749, 2069, 2981
PscI ACATGT 2 cut(s) 2018, 2163
PshAI GACNNNNGTC 1 cut(s) 407
PshBI ATTAAT 1 cut(s) 822
PsiI TTATAA 1 cut(s) 2925
Psp5II RGGWCCY 4 cut(s) 892, 1749, 2069, 2981
Psp6I CCWGG 6 cut(s) 469, 2149, 2677, 2945, 2971, 3298
PspFI CCCAGC 1 cut(s) 1745
PspGI CCWGG 6 cut(s) 469, 2149, 2677, 2945, 2971, 3298
PspPI GGNCC 6 cut(s) 268, 618, 892, 1749, 2069, 2981
PspPPI RGGWCCY 4 cut(s) 892, 1749, 2069, 2981
PstI CTGCAG 2 cut(s) 967, 1714
PstNI CAGNNNCTG 5 cut(s) 179, 515, 707, 2156, 2231
PsuI RGATCY 5 cut(s) 1166, 1816, 2153, 2228, 2891
RsaI GTAC 7 cut(s) 15, 207, 243, 747, 768, 1953, 2401
RsaNI GTAC 7 cut(s) 14, 206, 242, 746, 767, 1952, 2400
RseI CAYNNNNRTG 2 cut(s) 2640, 2691
SaqAI TTAA 8 cut(s) 249, 653, 822, 1377, 2127, 2316, 2862, 3640
SatI GCNGC 8 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2293, 3475
Sau96I GGNCC 6 cut(s) 268, 618, 892, 1749, 2069, 2981
SbfI CCTGCAGG 1 cut(s) 1714
SchI GAGTC 8 cut(s) 1907, 1952, 2036, 2111, 2155, 2375, 2489, 3308
ScrFI CCNGG 6 cut(s) 471, 2151, 2679, 2947, 2973, 3300
SdaI CCTGCAGG 1 cut(s) 1714
SduI GDGCHC 2 cut(s) 1115, 1466
SfcI CTRYAG 3 cut(s) 963, 1710, 2608
Sfr274I CTCGAG 1 cut(s) 3597
SfuI TTCGAA 1 cut(s) 2210
SinI GGWCC 5 cut(s) 268, 892, 1749, 2069, 2981
SlaI CTCGAG 1 cut(s) 3597
SmiMI CAYNNNNRTG 2 cut(s) 2640, 2691
SmlI CTYRAG 3 cut(s) 1126, 1760, 3597
SmoI CTYRAG 3 cut(s) 1126, 1760, 3597
SnaBI TACGTA 1 cut(s) 3610
SphI GCATGC 1 cut(s) 1230
Sse8387I CCTGCAGG 1 cut(s) 1714
SseBI AGGCCT 2 cut(s) 1278, 3595
SsiI CCGC 9 cut(s) 91, 429, 2276, 2698, 2833, 3281, 3289, 3475, 3517
SspMI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 3021
StuI AGGCCT 2 cut(s) 1278, 3595
StyD4I CCNGG 6 cut(s) 469, 2149, 2677, 2945, 2971, 3298
StyI CCWWGG 4 cut(s) 621, 978, 2475, 2635
TaiI ACGT 7 cut(s) 19, 480, 1005, 1795, 1986, 2361, 3612
TaqI TCGA 4 cut(s) 2210, 2325, 3120, 3598
TaqII GACCGA 1 cut(s) 1310
TatI WGTACW 2 cut(s) 205, 745
TauI GCSGC 1 cut(s) 3477
Tru1I TTAA 8 cut(s) 249, 653, 822, 1377, 2127, 2316, 2862, 3640
Tru9I TTAA 8 cut(s) 249, 653, 822, 1377, 2127, 2316, 2862, 3640
TscAI CASTG 6 cut(s) 893, 1195, 1444, 1831, 2032, 3403
TseI GCWGC 7 cut(s) 515, 962, 1643, 1739, 1742, 2033, 2292
TspGWI ACGGA 5 cut(s) 112, 267, 2191, 2721, 3457
TspRI CASTG 6 cut(s) 893, 1195, 1444, 1831, 2032, 3403
Van91I CCANNNNNTGG 1 cut(s) 1682
VpaK11BI GGWCC 5 cut(s) 268, 892, 1749, 2069, 2981
VspI ATTAAT 1 cut(s) 822
XagI CCTNNNNNAGG 1 cut(s) 2976
XapI RAATTY 6 cut(s) 442, 677, 3151, 3217, 3340, 3380
XbaI TCTAGA 2 cut(s) 1444, 3020
XceI RCATGY 7 cut(s) 242, 1230, 1334, 2022, 2167, 2186, 2312
XcmI CCANNNNNNNNNTGG 1 cut(s) 1925
XhoI CTCGAG 1 cut(s) 3597
XmiI GTMKAC 1 cut(s) 2998
XmnI GAANNNNTTC 1 cut(s) 670
XspI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 3021
Zsp2I ATGCAT 5 cut(s) 604, 700, 1228, 1232, 1704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.