pycom07g25270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001112
Physical Location & Seq
Forward (+)
25652799 .. 25655701
2903 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25270.2

Sequence Viewer

Length: 2712 bp
ATGATGAATACAGTACGTGTAGAACTTACAAAATCTATTAGTCCTTACCTGAACTGGAAGGTTTCAAAGGGTTATCGGAGTGCATCAAGGCGGTCAAGGAAACCTGTTGATAAAAGCAAAAAAATGGGTGCAGAGCTAGCAGAAAAAAGTGCTAGAAGTGCATCAGAAACATCAGTTTCTGATTCAGAGAAGCTTGGTGTGGCTGTACTTGGGAGACGATTTGGTGACAAAATTGAGCATGTACCGATTAAGAAAAGGAGACTTATGGTCCGGTCTCCGTCACCCCCTACACACCTTGAAGGCAACAAACCACTATTGGATGGTCGTCATTCTTCAGGTCACAAGTCTTGTCCAAAATCAGTTGGCAAAAAGCATCCCACAAGGAGTGATACTTCTACCCTGACTAGGGTCTGTCATAGTGCCAGTAGCGGTGCTAATGAGAATTTGAATGATGTGACAAATCAAAAGCATGGTGACGTGGATGACTTTTCTGGTATTGAGATATTGGCAGATGCTGCCTGCAACAACATCATAAATGATGACATCAATCATGTTGTGAAGAATCCACTAGGTGAAGATTCACCTCTGGATGAAAAAGATGCATCAACTTCTGCACGGCCTTCGGAGCAAACAACCATTGTGTCAACTTCTTTAACAGTCATGAGAACTGCTTCAGAATTTTCAGAAGCAAGAGATGTATCAGTCTCTGCAATCTTGGAAGAAAGTATTGCATCATTGGAAACAGTACATTCATCCCCTAAAGATTTACAACGAGAAGATAAAATAGGAAGCGCCTCTTTAGAAGCTGATGGGTTCAAAATTAATACTAACAAAGCACATGACGAAGCAGAAGCAAGATTGAGTTCCTCTAAAGATGTTAGGTTCCACTGGGACCTAAATGTTAGTATGGATGCTTGGGAGGAACCTTGTGATTTGGCGATTGCTGATCCTAAAACAACTGCTGCAGATGATATCTCCATGGATAATAAGCAGGGTGCAAACCTCCAAGTTTCAGAAGCTAATGAGATACCTAAGGAGGAAGATGCAAAGAATGACATTGCAAGCACCGTGAAACCTCTGTCTGACAATGAGGAACAGGGATTGAAAGCGTGCCCTGAAGTTGAGTTGAGTTGTGGTAAATGTGTTTCTCCTGATAACGCTTTGGGATCTTCAAAAGATTCTGGTAGCGGTGCTAAAGCTTCCTCTCAGGATGTGAGTGTAGATGTATGCATTGATGGTTCCCCTTGTGCTGGATTTGATAACATCGCAGTCACAGGCCCTGTGTCTGAGGAGACCGATAAAACCCCAATTTCCAGCTTTTCTGTTGAGCATGTGGCTGGTGAACTACCAAGTGAAACTGTCTGTTCAGGAAGTGTTAAGGTTGAAAACCCTGCTTTAGCTTGTGTGCCAGAAGGGGCATCTTGTGAGATTGAAAGCACTGTTCTAGATGAAGATGGCAAGGGCTCTGGTGCAACATCTAGCGTCCATGATGATCCAGAATCTCTGGAAGAGACGATGGGAGTAGAAAGCTGTCACTCACTCGCACCCGTTCGCCTTGATTCTGATGATAAATCTGCATCAGGTGCATCTGTTGGAGAAGGCCGGTCGCTGGTGACTATAATTGCAAAGGAGCCTGTTGAAGCTGCCTCTGACACTCATACTGTCGATTCTCTGCCAAATGATGGTTCTGAGGAAGTAATGCATAAACCTTCAGGGAATCCCATGACGAATCAAGTAGCTGCTGCTGGGTCCTCCACTCTAGAGCAATGCCACTATGGTGAAGATAGCTCACGTAGCTTGGATAGAGTAACTAAAGATCCTTCCAGTGATGGTTATGACTTGAATACTCGTCAAGATGATAAGGATTACATGGTTGGCAAGGGAAACACTCTGGAACCTGAAGCTGGTTACGACTCCCAGTATGAAGATGGGGAGCTGAGGGAGTCGGATGTACCATATTGGGAGGAGAATGAAATTGATGATGTAGAAGCTGAATGTGTTGACTATGGTTCTGATACATGTGACAGTGAAGCTGCTGATGACTCTATATCAGGGAAAGTTGGAATGGGACCTGAATGCAGAGAAACAGAATTATTTGGGGAGTCAAGGAAAATTGACAGGAACATTAAGCTTGTGAGAGGATTGAGTCCAGGATCAGATAACATGTGTGACAAAAATGAACATGCTTTGAGACAATGTTCCGTTGGTTCGAAGACAAAAACTTCAGGATCGATCAACTACCTTGTGATTCTGAAGCTTCTTCAAACAGAACTGCGGAAGCAATTGAGGGCTGCACAGCAAGGAGACAAGCTGTCAGTGGCTTCGATGGCTATGATGCCAAACATTCTCCTGCCAACGTGGTTGGGTCAATGGCATCTGACTCCTCAAATAAGATGGGTACTGAATGTGCAAGGCGGAGGAGGTTGGGCAACTTTGATTCTATTCGCTCTGAGGAAGCTGGTTCTGATCAGTCCATGGGAAGGGAAAAGTATGACTCACGTATGCAGGGTAAAAGCTTCAGAGGTGTGGTTTATTCTTCAGGGAGTTATTGGGATTCCAAGCGACGCGAATCACCCACTTATCATGGTTCCTTTGGCTCTGGACGCTGTAGGCCAAGAAGTGTTGTTGAGAACCATGGATATGAGATGGAATCAGACGAAACATTTTCAGAAGCACCAGGAGTTCACAACCGTGTTCGCAGGCAAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

904

Amino Acids

97.44

Weight (kDa)

4.89

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1682
AccII CGCG 1 cut(s) 2567
AciI CCGC 5 cut(s) 91, 429, 1188, 2275, 2415
AclWI GGATC 6 cut(s) 941, 1174, 1487, 1811, 2161, 2236
AcsI RAATTY 2 cut(s) 442, 677
AcuI CTGAAG 9 cut(s) 318, 657, 1137, 1695, 1920, 2208, 2273, 2502, 2522
AdeI CACNNNGTG 1 cut(s) 572
AfaI GTAC 6 cut(s) 15, 207, 243, 747, 1953, 2400
AfiI CCNNNNNNNGG 7 cut(s) 405, 406, 1250, 1609, 1682, 1904, 2480
AflIII ACRYGT 3 cut(s) 16, 2018, 2163
AhdI GACNNNNNGTC 2 cut(s) 266, 2311
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 2 cut(s) 2149, 2676
AjuI GAANNNNNNNTTGG 2 cut(s) 1669, 1701
AleI CACNNNNGTG 2 cut(s) 1776, 2690
Alw26I GTCTC 8 cut(s) 208, 253, 279, 709, 1286, 1505, 2185, 2298
AlwI GGATC 6 cut(s) 941, 1174, 1487, 1811, 2161, 2236
AlwNI CAGNNNCTG 4 cut(s) 179, 515, 707, 1280
AoxI GGCC 4 cut(s) 617, 1276, 1600, 2611
ApeKI GCWGC 7 cut(s) 515, 962, 1643, 1739, 1742, 2033, 2291
ApoI RAATTY 2 cut(s) 442, 677
AseI ATTAAT 1 cut(s) 822
Asp700I GAANNNNTTC 1 cut(s) 670
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 5 cut(s) 268, 892, 1277, 1749, 2069
AsuHPI GGTGA 9 cut(s) 236, 273, 485, 573, 584, 1352, 1624, 1790, 2564
AsuII TTCGAA 1 cut(s) 2210
AsuNHI GCTAGC 1 cut(s) 136
AvaII GGWCC 4 cut(s) 268, 892, 1749, 2069
AxyI CCTNAGG 1 cut(s) 1032
BaeGI GKGCMC 1 cut(s) 1115
BanII GRGCYC 1 cut(s) 1466
BbsI GAAGAC 1 cut(s) 2219
BbvCI CCTCAGC 1 cut(s) 1937
BbvI GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2278
BceAI ACGGC 1 cut(s) 632
BcgI CGANNNNNNTGC 2 cut(s) 603, 637
BciT130I CCWGG 2 cut(s) 2151, 2678
BclI TGATCA 1 cut(s) 2466
BcoDI GTCTC 8 cut(s) 208, 253, 279, 709, 1286, 1505, 2185, 2298
BfaI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 1760
BfmI CTRYAG 2 cut(s) 963, 2607
BfoI RGCGCY 1 cut(s) 795
BisI GCNGC 7 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2292
BlsI GCNGC 7 cut(s) 517, 964, 1645, 1741, 1744, 2035, 2293
Bme1390I CCNGG 2 cut(s) 2151, 2678
Bme18I GGWCC 4 cut(s) 268, 892, 1749, 2069
BmeRI GACNNNNNGTC 2 cut(s) 266, 2311
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 5 cut(s) 268, 892, 1277, 1749, 2069
BmiI GGNNCC 9 cut(s) 884, 893, 924, 1240, 1632, 1750, 1896, 2070, 2589
BmrFI CCNGG 2 cut(s) 2151, 2678
BmrI ACTGGG 2 cut(s) 898, 1912
BmtI GCTAGC 1 cut(s) 140
BmuI ACTGGG 2 cut(s) 898, 1912
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 2219
Bpu10I CCTNAGC 1 cut(s) 1937
Bpu14I TTCGAA 1 cut(s) 2210
Bsa29I ATCGAT 1 cut(s) 2231
BsaAI YACGTR 3 cut(s) 17, 1793, 2500
BsaI GGTCTC 2 cut(s) 279, 1286
BsaJI CCNNGG 3 cut(s) 978, 2474, 2634
BsaWI WCCGGW 1 cut(s) 270
Bsc4I CCNNNNNNNGG 7 cut(s) 405, 406, 1250, 1609, 1682, 1904, 2480
Bse118I RCCGGY 1 cut(s) 1602
Bse1I ACTGG 5 cut(s) 59, 423, 893, 1824, 1918
Bse21I CCTNAGG 1 cut(s) 1032
Bse3DI GCAATG 2 cut(s) 1056, 1772
BseBI CCWGG 2 cut(s) 2151, 2678
BseCI ATCGAT 1 cut(s) 2231
BseDI CCNNGG 3 cut(s) 978, 2474, 2634
BseGI GGATG 8 cut(s) 325, 373, 487, 595, 752, 916, 1216, 1954
BseLI CCNNNNNNNGG 7 cut(s) 405, 406, 1250, 1609, 1682, 1904, 2480
BseMI GCAATG 2 cut(s) 1056, 1772
BseMII CTCAG 5 cut(s) 1220, 1278, 1680, 1928, 2441
BseNI ACTGG 5 cut(s) 59, 423, 893, 1824, 1918
BseRI GAGGAG 4 cut(s) 1304, 1979, 2373, 2433
BseSI GKGCMC 1 cut(s) 1115
BseXI GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2278
BseYI CCCAGC 1 cut(s) 1745
BsgI GTGCAG 3 cut(s) 150, 597, 2277
Bsh1236I CGCG 1 cut(s) 2567
Bsh1285I CGRYCG 1 cut(s) 1607
BshFI GGCC 4 cut(s) 619, 1278, 1602, 2613
BshVI ATCGAT 1 cut(s) 2231
BsiEI CGRYCG 1 cut(s) 1607
BsiSI CCGG 2 cut(s) 271, 1603
BslFI GGGAC 2 cut(s) 905, 2082
BslI CCNNNNNNNGG 7 cut(s) 405, 406, 1250, 1609, 1682, 1904, 2480
BsmAI GTCTC 8 cut(s) 208, 253, 279, 709, 1286, 1505, 2185, 2298
BsmBI CGTCTC 2 cut(s) 208, 1505
BsmFI GGGAC 2 cut(s) 905, 2082
BsmI GAATGC 1 cut(s) 2081
BsnI GGCC 4 cut(s) 619, 1278, 1602, 2613
Bso31I GGTCTC 2 cut(s) 279, 1286
Bsp119I TTCGAA 1 cut(s) 2210
Bsp1286I GDGCHC 2 cut(s) 1115, 1466
Bsp143I GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
Bsp19I CCATGG 3 cut(s) 978, 2474, 2634
BspACI CCGC 5 cut(s) 91, 429, 1188, 2275, 2415
BspANI GGCC 4 cut(s) 619, 1278, 1602, 2613
BspCNI CTCAG 5 cut(s) 1219, 1279, 1681, 1929, 2442
BspDI ATCGAT 1 cut(s) 2231
BspFNI CGCG 1 cut(s) 2567
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 9 cut(s) 884, 893, 924, 1240, 1632, 1750, 1896, 2070, 2589
BspMAI CTGCAG 1 cut(s) 967
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 6 cut(s) 941, 1174, 1487, 1811, 2161, 2236
BspT104I TTCGAA 1 cut(s) 2210
BspTNI GGTCTC 2 cut(s) 279, 1286
BsrDI GCAATG 2 cut(s) 1056, 1772
BsrFI RCCGGY 1 cut(s) 1602
BsrI ACTGG 5 cut(s) 59, 423, 893, 1824, 1918
BssAI RCCGGY 1 cut(s) 1602
BssECI CCNNGG 3 cut(s) 978, 2474, 2634
BssMI GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
BssT1I CCWWGG 3 cut(s) 978, 2474, 2634
Bst2UI CCWGG 2 cut(s) 2151, 2678
Bst4CI ACNGT 9 cut(s) 13, 658, 745, 1069, 1360, 1441, 1663, 2027, 2692
Bst6I CTCTTC 1 cut(s) 1503
BstAPI GCANNNNNTGC 2 cut(s) 515, 1583
BstBAI YACGTR 3 cut(s) 17, 1793, 2500
BstBI TTCGAA 1 cut(s) 2210
BstC8I GCNNGC 6 cut(s) 138, 520, 1063, 1111, 2701, 2705
BstDEI CTNAG 6 cut(s) 1032, 1206, 1287, 1689, 1937, 2450
BstDSI CCRYGG 3 cut(s) 978, 2474, 2634
BstF5I GGATG 8 cut(s) 325, 373, 487, 595, 752, 916, 1216, 1954
BstFNI CGCG 1 cut(s) 2567
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 8 cut(s) 949, 1169, 1495, 1819, 2156, 2231, 2235, 2469
BstMAI GTCTC 8 cut(s) 208, 253, 279, 709, 1286, 1505, 2185, 2298
BstMBI GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
BstMCI CGRYCG 1 cut(s) 1607
BstMWI GCNNNNNNNGC 8 cut(s) 137, 158, 515, 625, 1583, 1794, 2327, 2603
BstNI CCWGG 2 cut(s) 2151, 2678
BstNSI RCATGY 5 cut(s) 242, 1334, 2022, 2167, 2186
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 2 cut(s) 2149, 2676
BstSFI CTRYAG 2 cut(s) 963, 2607
BstSLI GKGCMC 1 cut(s) 1115
BstUI CGCG 1 cut(s) 2567
BstV1I GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2278
BstV2I GAAGAC 1 cut(s) 2219
BstX2I RGATCY 2 cut(s) 1166, 1816
BstYI RGATCY 2 cut(s) 1166, 1816
Bsu15I ATCGAT 1 cut(s) 2231
Bsu36I CCTNAGG 1 cut(s) 1032
BsuRI GGCC 4 cut(s) 619, 1278, 1602, 2613
BsuTUI ATCGAT 1 cut(s) 2231
BtgI CCRYGG 3 cut(s) 978, 2474, 2634
BtgZI GCGATG 1 cut(s) 1249
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 8 cut(s) 325, 373, 487, 595, 752, 916, 1216, 1954
BtsIMutI CAGTG 5 cut(s) 886, 1437, 1831, 2032, 2322
Cac8I GCNNGC 6 cut(s) 138, 520, 1063, 1111, 2701, 2705
CaiI CAGNNNCTG 4 cut(s) 179, 515, 707, 1280
CciI TCATGA 1 cut(s) 660
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 1 cut(s) 1602
Cfr13I GGNCC 5 cut(s) 268, 892, 1277, 1749, 2069
ClaI ATCGAT 1 cut(s) 2231
CseI GACGC 3 cut(s) 1471, 2573, 2612
Csp6I GTAC 6 cut(s) 14, 206, 242, 746, 1952, 2399
CviQI GTAC 6 cut(s) 14, 206, 242, 746, 1952, 2399
DdeI CTNAG 6 cut(s) 1032, 1206, 1287, 1689, 1937, 2450
DpnI GATC 8 cut(s) 948, 1168, 1494, 1818, 2155, 2230, 2234, 2468
DpnII GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
DraIII CACNNNGTG 1 cut(s) 572
DriI GACNNNNNGTC 2 cut(s) 266, 2311
Eam1104I CTCTTC 1 cut(s) 1503
Eam1105I GACNNNNNGTC 2 cut(s) 266, 2311
EarI CTCTTC 1 cut(s) 1503
EciI GGCGGA 1 cut(s) 2430
Eco130I CCWWGG 3 cut(s) 978, 2474, 2634
Eco24I GRGCYC 1 cut(s) 1466
Eco31I GGTCTC 2 cut(s) 279, 1286
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 4 cut(s) 268, 892, 1749, 2069
Eco57I CTGAAG 9 cut(s) 318, 657, 1137, 1695, 1920, 2208, 2273, 2502, 2522
Eco81I CCTNAGG 1 cut(s) 1032
EcoO109I RGGNCCY 4 cut(s) 892, 1277, 1749, 2069
EcoRII CCWGG 2 cut(s) 2149, 2676
EcoRV GATATC 1 cut(s) 973
EcoT14I CCWWGG 3 cut(s) 978, 2474, 2634
EcoT22I ATGCAT 3 cut(s) 604, 1232, 1704
EcoT38I GRGCYC 1 cut(s) 1466
ErhI CCWWGG 3 cut(s) 978, 2474, 2634
Esp3I CGTCTC 2 cut(s) 208, 1505
FalI AAGNNNNNCTT 2 cut(s) 781, 813
FaqI GGGAC 2 cut(s) 905, 2082
FbaI TGATCA 1 cut(s) 2466
Fnu4HI GCNGC 7 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2292
FokI GGATG 8 cut(s) 332, 360, 494, 602, 739, 923, 1223, 1961
FriOI GRGCYC 1 cut(s) 1466
Fsp4HI GCNGC 7 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2292
FspBI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 1760
GlaI GCGC 1 cut(s) 793
GluI GCNGC 7 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2292
GsaI CCCAGC 1 cut(s) 1749
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 4 cut(s) 619, 1278, 1602, 2613
HapII CCGG 2 cut(s) 271, 1603
HgaI GACGC 3 cut(s) 1471, 2573, 2612
HhaI GCGC 1 cut(s) 794
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HincII GTYRAC 2 cut(s) 645, 2002
HindII GTYRAC 2 cut(s) 645, 2002
HindIII AAGCTT 5 cut(s) 191, 1197, 2129, 2255, 2514
HpaII CCGG 2 cut(s) 271, 1603
HphI GGTGA 9 cut(s) 236, 273, 485, 573, 584, 1352, 1624, 1790, 2564
Hpy166II GTNNAC 4 cut(s) 645, 1343, 2002, 2685
Hpy8I GTNNAC 4 cut(s) 645, 1343, 2002, 2685
Hpy99I CGWCG 1 cut(s) 2567
HpyAV CCTTC 8 cut(s) 52, 293, 630, 1406, 1592, 1719, 1830, 2474
HpyCH4III ACNGT 9 cut(s) 13, 658, 745, 1069, 1360, 1441, 1663, 2027, 2692
HpyCH4IV ACGT 5 cut(s) 16, 477, 1792, 2357, 2499
HpyF10VI GCNNNNNNNGC 8 cut(s) 137, 158, 515, 625, 1583, 1794, 2327, 2603
HpyF3I CTNAG 6 cut(s) 1032, 1206, 1287, 1689, 1937, 2450
HpySE526I ACGT 5 cut(s) 16, 477, 1792, 2357, 2499
HspAI GCGC 1 cut(s) 792
Ksp22I TGATCA 1 cut(s) 2466
Kzo9I GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
LmnI GCTCC 3 cut(s) 625, 1630, 1933
Lsp1109I GCAGC 7 cut(s) 502, 949, 1630, 1726, 1729, 2020, 2278
MaeI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 1760
MaeII ACGT 5 cut(s) 16, 477, 1792, 2357, 2499
MalI GATC 8 cut(s) 948, 1168, 1494, 1818, 2155, 2230, 2234, 2468
MboI GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
MfeI CAATTG 1 cut(s) 2282
MflI RGATCY 2 cut(s) 1166, 1816
MhlI GDGCHC 2 cut(s) 1115, 1466
MlyI GAGTC 7 cut(s) 1907, 1952, 2036, 2111, 2155, 2374, 2488
MmeI TCCRAC 3 cut(s) 1573, 1926, 2041
Mph1103I ATGCAT 3 cut(s) 604, 1232, 1704
MroXI GAANNNNTTC 1 cut(s) 670
MseI TTAA 5 cut(s) 249, 653, 822, 1377, 2127
MslI CAYNNNNRTG 3 cut(s) 1776, 2639, 2690
MspI CCGG 2 cut(s) 271, 1603
MspR9I CCNGG 2 cut(s) 2151, 2678
MunI CAATTG 1 cut(s) 2282
Mva1269I GAATGC 1 cut(s) 2081
MvaI CCWGG 2 cut(s) 2151, 2678
MvnI CGCG 1 cut(s) 2567
MwoI GCNNNNNNNGC 8 cut(s) 137, 158, 515, 625, 1583, 1794, 2327, 2603
NcoI CCATGG 3 cut(s) 978, 2474, 2634
NdeII GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
NheI GCTAGC 1 cut(s) 136
NlaIV GGNNCC 9 cut(s) 884, 893, 924, 1240, 1632, 1750, 1896, 2070, 2589
NsiI ATGCAT 3 cut(s) 604, 1232, 1704
NspI RCATGY 5 cut(s) 242, 1334, 2022, 2167, 2186
NspV TTCGAA 1 cut(s) 2210
OliI CACNNNNGTG 2 cut(s) 1776, 2690
PagI TCATGA 1 cut(s) 660
PciI ACATGT 2 cut(s) 2018, 2163
PctI GAATGC 1 cut(s) 2081
PdmI GAANNNNTTC 1 cut(s) 670
PflMI CCANNNNNTGG 1 cut(s) 1682
PfoI TCCNGGA 1 cut(s) 2149
PkrI GCNGC 7 cut(s) 517, 964, 1645, 1741, 1744, 2035, 2293
PleI GAGTC 7 cut(s) 1907, 1951, 2036, 2110, 2154, 2374, 2488
PpsI GAGTC 7 cut(s) 1907, 1951, 2036, 2110, 2154, 2374, 2488
Ppu21I YACGTR 3 cut(s) 17, 1793, 2500
PpuMI RGGWCCY 3 cut(s) 892, 1749, 2069
PscI ACATGT 2 cut(s) 2018, 2163
PshAI GACNNNNGTC 1 cut(s) 407
PshBI ATTAAT 1 cut(s) 822
Psp5II RGGWCCY 3 cut(s) 892, 1749, 2069
Psp6I CCWGG 2 cut(s) 2149, 2676
PspFI CCCAGC 1 cut(s) 1745
PspGI CCWGG 2 cut(s) 2149, 2676
PspN4I GGNNCC 9 cut(s) 884, 893, 924, 1240, 1632, 1750, 1896, 2070, 2589
PspPI GGNCC 5 cut(s) 268, 892, 1277, 1749, 2069
PspPPI RGGWCCY 3 cut(s) 892, 1749, 2069
PstI CTGCAG 1 cut(s) 967
PstNI CAGNNNCTG 4 cut(s) 179, 515, 707, 1280
PsuI RGATCY 2 cut(s) 1166, 1816
RsaI GTAC 6 cut(s) 15, 207, 243, 747, 1953, 2400
RsaNI GTAC 6 cut(s) 14, 206, 242, 746, 1952, 2399
RseI CAYNNNNRTG 3 cut(s) 1776, 2639, 2690
SaqAI TTAA 5 cut(s) 249, 653, 822, 1377, 2127
SatI GCNGC 7 cut(s) 516, 963, 1644, 1740, 1743, 2034, 2292
Sau3AI GATC 8 cut(s) 946, 1166, 1492, 1816, 2153, 2228, 2232, 2466
Sau96I GGNCC 5 cut(s) 268, 892, 1277, 1749, 2069
SchI GAGTC 7 cut(s) 1907, 1952, 2036, 2111, 2155, 2374, 2488
ScrFI CCNGG 2 cut(s) 2151, 2678
SduI GDGCHC 2 cut(s) 1115, 1466
SfcI CTRYAG 2 cut(s) 963, 2607
SfuI TTCGAA 1 cut(s) 2210
SinI GGWCC 4 cut(s) 268, 892, 1749, 2069
SmiMI CAYNNNNRTG 3 cut(s) 1776, 2639, 2690
SsiI CCGC 5 cut(s) 91, 429, 1188, 2275, 2415
SspMI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 1760
StyD4I CCNGG 2 cut(s) 2149, 2676
StyI CCWWGG 3 cut(s) 978, 2474, 2634
TaaI ACNGT 9 cut(s) 13, 658, 745, 1069, 1360, 1441, 1663, 2027, 2692
TaiI ACGT 5 cut(s) 19, 480, 1795, 2360, 2502
TaqI TCGA 4 cut(s) 1665, 2210, 2231, 2324
TaqII GACCGA 1 cut(s) 1310
TatI WGTACW 2 cut(s) 205, 745
Tru1I TTAA 5 cut(s) 249, 653, 822, 1377, 2127
Tru9I TTAA 5 cut(s) 249, 653, 822, 1377, 2127
TscAI CASTG 5 cut(s) 893, 1444, 1831, 2032, 2322
TseI GCWGC 7 cut(s) 515, 962, 1643, 1739, 1742, 2033, 2291
TspDTI ATGAA 7 cut(s) 20, 606, 741, 1464, 1938, 1986, 2193
TspGWI ACGGA 2 cut(s) 267, 2191
TspRI CASTG 5 cut(s) 893, 1444, 1831, 2032, 2322
Van91I CCANNNNNTGG 1 cut(s) 1682
VpaK11BI GGWCC 4 cut(s) 268, 892, 1749, 2069
VspI ATTAAT 1 cut(s) 822
XapI RAATTY 2 cut(s) 442, 677
XbaI TCTAGA 2 cut(s) 1444, 1759
XceI RCATGY 5 cut(s) 242, 1334, 2022, 2167, 2186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1925
XmnI GAANNNNTTC 1 cut(s) 670
XspI CTAG 7 cut(s) 137, 153, 405, 569, 1445, 1479, 1760
Zsp2I ATGCAT 3 cut(s) 604, 1232, 1704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.