pycom01g21760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001112
Physical Location & Seq
Forward (+)
19656958 .. 19658319
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21760.3

Sequence Viewer

Length: 1281 bp
ATGGCATCTGACTCCTCAAATAAGATGGGTACTCAATGTGCAAGAAGGAGGAGGTTGGGCAACTTTGATTCTATTCGCTCCGAGGAAGCTGGTTCCGATCAATCCATGGGAAGGGAAAAGTCTGATTCACGTATGCAGGGTAAAAGTTTCGGAGATGTGGTTAAATCTTCAGGGAGTTATTGGGATTCCAAGGGCCGCGAATCACCCACTTATCGTGGTTCCTTTGGTTCTGGACGCTCTAGACCAAGAATTGTTGTTGAGAACCATGGTTATGAGATGGAATCAGACGACACATTTTCAGAAGCAGCAGGAGTTCACAACCGTGTTCGCAGGCCAGCTATAACTTTTTCATCAAATCGTTCGTATCATCCCGCGTTTAGAAGATCATCACCATCTGAAAGGAATAATGCACACAATATGCATAGGGGAATGATACCTATGAAGATACAAGTCCTGATAGACGAAGGTTTAGACGATATCCACAAGGGGTTAACAGAGGCATCAGGGAGGAGTACCACAGGCCCATGCCCGATCGATCCCAACGAATGCTCTTATAATGTGCCACGACGAATGCCCAGGAGAGAAGAAAGCACTTCACCCCCTGGCAGAGGACCTATATATTATAGTAGACCCTATCAGAAGCCCCAGTCTCGATGCAGAAGTCGCTCTCCTCTTGGATGGGGTTTACCGAGAGAACGGAATGATATTTCAAGACATCGTGGTAGCAGGTCTCCTGATTATAGGTTTGATTCTAATATGGAGCTGAGGGTGCCTTTCCAAAGGCAAAATTTCGGAGGCAAGTATGATGTAGGGTTCGTGTCCCCACCAAAGCGTCGGTTTTCCCCACAACAGAATTCCAGATGGTTTGATGATTCACATAGAAGTGTAGATCATAATATCAGGGGCGGGAGATTTGCTGGAAGACGGTTCCAGCCAGGACAGAGGTTTGACTCAGAGCGTTCATCTCGGAGATTGAATCCAGATGGTTACTCTGAGCCCGTGATGCGTCCTGCAAGATACTCTGAGTTGTCCAGTGGTGGTAGGGAGTGTAGATACGAAGGTAGTGATGATGATAGAAGGAAACCTGATGGAAGATATGAGATCGTTCATCGTGTGAGGCGTTTTGATTCAGATGGTGGTGTGCGTCAGTACCGTTATGATGAAGAAGATCGTTTTGCATCTCACAACACGCAGAACTACGATGAATCTGACAATAGGGCAGCCGAGAGACGGCCTCGAGAAGCTTACGTAGGAGTAGCAGCGAAGAGGAGAGTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

49.08

Weight (kDa)

10.09

Isoelectric Point (pI)

85.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
Acc36I ACCTGC 1 cut(s) 717
AccB1I GGYRCC 1 cut(s) 769
AccI GTMKAC 1 cut(s) 628
AccII CGCG 2 cut(s) 198, 374
AciI CCGC 3 cut(s) 196, 372, 906
AclWI GGATC 1 cut(s) 530
AcsI RAATTY 2 cut(s) 787, 853
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 3 cut(s) 31, 514, 1151
AfiI CCNNNNNNNGG 3 cut(s) 111, 608, 1230
AgsI TTSAA 2 cut(s) 711, 976
AjnI CCWGG 3 cut(s) 575, 601, 934
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 4 cut(s) 89, 338, 763, 1244
AluI AGCT 4 cut(s) 89, 338, 763, 1244
Alw26I GTCTC 3 cut(s) 654, 735, 1222
AlwI GGATC 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 1236
AoxI GGCC 4 cut(s) 193, 332, 520, 1232
ApeKI GCWGC 3 cut(s) 305, 1220, 1259
ApoI RAATTY 2 cut(s) 787, 853
AspS9I GGNCC 3 cut(s) 193, 521, 611
AsuHPI GGTGA 3 cut(s) 195, 381, 588
AvaI CYCGRG 1 cut(s) 1236
AvaII GGWCC 1 cut(s) 611
BaeI ACNNNNGTAYC 2 cut(s) 1045, 1078
BanI GGYRCC 1 cut(s) 769
BanII GRGCYC 1 cut(s) 999
BbsI GAAGAC 1 cut(s) 928
BbvCI CCTCAGC 1 cut(s) 764
BbvI GCAGC 3 cut(s) 317, 1232, 1271
BccI CCATC 8 cut(s) 19, 271, 400, 672, 855, 977, 1082, 1127
BceAI ACGGC 1 cut(s) 1247
BciT130I CCWGG 3 cut(s) 577, 603, 936
BcoDI GTCTC 3 cut(s) 654, 735, 1222
BfaI CTAG 1 cut(s) 240
BfuAI ACCTGC 1 cut(s) 717
BisI GCNGC 4 cut(s) 196, 306, 1221, 1260
BlsI GCNGC 4 cut(s) 197, 307, 1222, 1261
Bme1390I CCNGG 3 cut(s) 577, 603, 936
Bme18I GGWCC 1 cut(s) 611
BmeT110I CYCGRG 1 cut(s) 1236
BmgT120I GGNCC 3 cut(s) 193, 521, 611
BmiI GGNNCC 4 cut(s) 94, 220, 771, 929
BmrFI CCNGG 3 cut(s) 577, 603, 936
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 5 cut(s) 14, 509, 644, 993, 1187
BmuI ACTGGG 1 cut(s) 640
BpiI GAAGAC 1 cut(s) 928
BplI GAGNNNNNCTC 2 cut(s) 1219, 1251
Bpu10I CCTNAGC 1 cut(s) 764
Bsa29I ATCGAT 1 cut(s) 534
BsaAI YACGTR 2 cut(s) 131, 1249
BsaI GGTCTC 1 cut(s) 735
BsaJI CCNNGG 6 cut(s) 81, 105, 189, 265, 575, 601
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 608, 1230
Bse1I ACTGG 2 cut(s) 646, 1032
BseBI CCWGG 3 cut(s) 577, 603, 936
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 6 cut(s) 81, 105, 189, 265, 575, 601
BseGI GGATG 2 cut(s) 367, 683
BseLI CCNNNNNNNGG 3 cut(s) 111, 608, 1230
BseMII CTCAG 4 cut(s) 755, 966, 984, 1014
BseNI ACTGG 2 cut(s) 646, 1032
BseRI GAGGAG 4 cut(s) 4, 64, 523, 660
BseXI GCAGC 3 cut(s) 317, 1232, 1271
Bsh1236I CGCG 2 cut(s) 198, 374
Bsh1285I CGRYCG 1 cut(s) 534
BshFI GGCC 4 cut(s) 195, 334, 522, 1234
BshNI GGYRCC 1 cut(s) 769
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 1 cut(s) 534
BsiHKCI CYCGRG 1 cut(s) 1236
BslFI GGGAC 1 cut(s) 805
BslI CCNNNNNNNGG 3 cut(s) 111, 608, 1230
BsmAI GTCTC 3 cut(s) 654, 735, 1222
BsmBI CGTCTC 1 cut(s) 1222
BsmFI GGGAC 1 cut(s) 805
BsmI GAATGC 2 cut(s) 551, 576
BsnI GGCC 4 cut(s) 195, 334, 522, 1234
Bso31I GGTCTC 1 cut(s) 735
BsoBI CYCGRG 1 cut(s) 1236
Bsp1286I GDGCHC 1 cut(s) 999
Bsp143I GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
Bsp19I CCATGG 2 cut(s) 105, 265
BspACI CCGC 3 cut(s) 196, 372, 906
BspANI GGCC 4 cut(s) 195, 334, 522, 1234
BspCNI CTCAG 4 cut(s) 756, 965, 985, 1015
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 2 cut(s) 198, 374
BspLI GGNNCC 4 cut(s) 94, 220, 771, 929
BspMI ACCTGC 1 cut(s) 717
BspPI GGATC 1 cut(s) 530
BspT107I GGYRCC 1 cut(s) 769
BspTNI GGTCTC 1 cut(s) 735
BsrI ACTGG 2 cut(s) 646, 1032
BssECI CCNNGG 6 cut(s) 81, 105, 189, 265, 575, 601
BssMI GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
BssT1I CCWWGG 3 cut(s) 105, 189, 265
Bst2UI CCWGG 3 cut(s) 577, 603, 936
Bst4CI ACNGT 3 cut(s) 323, 927, 1154
Bst6I CTCTTC 1 cut(s) 1259
BstBAI YACGTR 2 cut(s) 131, 1249
BstC8I GCNNGC 2 cut(s) 332, 336
BstDEI CTNAG 4 cut(s) 764, 952, 993, 1023
BstDSI CCRYGG 2 cut(s) 105, 265
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 2 cut(s) 367, 683
BstFNI CGCG 2 cut(s) 198, 374
BstKTI GATC 7 cut(s) 100, 386, 534, 538, 892, 1104, 1171
BstMAI GTCTC 3 cut(s) 654, 735, 1222
BstMBI GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
BstMCI CGRYCG 1 cut(s) 534
BstMWI GCNNNNNNNGC 3 cut(s) 663, 769, 1003
BstNI CCWGG 3 cut(s) 577, 603, 936
BstSCI CCNGG 3 cut(s) 575, 601, 934
BstSNI TACGTA 1 cut(s) 1249
BstUI CGCG 2 cut(s) 198, 374
BstV1I GCAGC 3 cut(s) 317, 1232, 1271
BstV2I GAAGAC 1 cut(s) 928
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 4 cut(s) 195, 334, 522, 1234
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 2 cut(s) 105, 265
BtsCI GGATG 2 cut(s) 367, 683
BtsIMutI CAGTG 1 cut(s) 1039
BveI ACCTGC 1 cut(s) 717
Cac8I GCNNGC 2 cut(s) 332, 336
Cfr13I GGNCC 3 cut(s) 193, 521, 611
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 4 cut(s) 243, 821, 995, 1133
Csp6I GTAC 3 cut(s) 30, 513, 1150
CviAII CATG 3 cut(s) 106, 266, 525
CviQI GTAC 3 cut(s) 30, 513, 1150
DdeI CTNAG 4 cut(s) 764, 952, 993, 1023
DpnI GATC 7 cut(s) 99, 385, 533, 537, 891, 1103, 1170
DpnII GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
Eam1104I CTCTTC 1 cut(s) 1259
EarI CTCTTC 1 cut(s) 1259
Eco105I TACGTA 1 cut(s) 1249
Eco130I CCWWGG 3 cut(s) 105, 189, 265
Eco24I GRGCYC 1 cut(s) 999
Eco31I GGTCTC 1 cut(s) 735
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 1236
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 3 cut(s) 575, 601, 934
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 3 cut(s) 105, 189, 265
EcoT22I ATGCAT 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 3 cut(s) 105, 189, 265
Esp3I CGTCTC 1 cut(s) 1222
FaeI CATG 3 cut(s) 109, 269, 528
FaqI GGGAC 1 cut(s) 805
FatI CATG 3 cut(s) 105, 265, 524
FauI CCCGC 2 cut(s) 379, 899
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 4 cut(s) 196, 306, 1221, 1260
FokI GGATG 2 cut(s) 354, 690
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 4 cut(s) 196, 306, 1221, 1260
FspBI CTAG 1 cut(s) 240
GluI GCNGC 4 cut(s) 196, 306, 1221, 1260
HaeIII GGCC 4 cut(s) 195, 334, 522, 1234
HgaI GACGC 4 cut(s) 243, 821, 995, 1133
Hin1II CATG 3 cut(s) 109, 269, 528
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HindIII AAGCTT 1 cut(s) 1242
HpaI GTTAAC 1 cut(s) 492
HphI GGTGA 3 cut(s) 195, 381, 588
Hpy166II GTNNAC 4 cut(s) 316, 492, 629, 686
Hpy188III TCNNGA 9 cut(s) 231, 240, 454, 651, 711, 734, 858, 980, 1238
Hpy8I GTNNAC 4 cut(s) 316, 492, 629, 686
Hpy99I CGWCG 2 cut(s) 570, 837
HpyAV CCTTC 5 cut(s) 39, 105, 458, 1052, 1071
HpyCH4III ACNGT 3 cut(s) 323, 927, 1154
HpyCH4IV ACGT 2 cut(s) 130, 1248
HpyCH4V TGCA 7 cut(s) 41, 136, 410, 421, 657, 1013, 1178
HpyF10VI GCNNNNNNNGC 3 cut(s) 663, 769, 1003
HpyF3I CTNAG 4 cut(s) 764, 952, 993, 1023
HpySE526I ACGT 2 cut(s) 130, 1248
Hsp92II CATG 3 cut(s) 109, 269, 528
KspAI GTTAAC 1 cut(s) 492
Kzo9I GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
LmnI GCTCC 2 cut(s) 83, 760
Lsp1109I GCAGC 3 cut(s) 317, 1232, 1271
LweI GCATC 5 cut(s) 14, 509, 644, 993, 1187
MaeI CTAG 1 cut(s) 240
MaeII ACGT 2 cut(s) 130, 1248
MaeIII GTNAC 1 cut(s) 986
MalI GATC 7 cut(s) 99, 385, 533, 537, 891, 1103, 1170
MboI GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
MboII GAAGA 9 cut(s) 159, 393, 454, 596, 933, 1104, 1175, 1178, 1276
MhlI GDGCHC 1 cut(s) 999
MluCI AATT 4 cut(s) 249, 787, 853, 1276
MlyI GAGTC 2 cut(s) 5, 944
Mph1103I ATGCAT 1 cut(s) 423
MseI TTAA 3 cut(s) 162, 491, 1279
MslI CAYNNNNRTG 3 cut(s) 270, 321, 882
MspR9I CCNGG 3 cut(s) 577, 603, 936
Mva1269I GAATGC 2 cut(s) 551, 576
MvaI CCWGG 3 cut(s) 577, 603, 936
MvnI CGCG 2 cut(s) 198, 374
MwoI GCNNNNNNNGC 3 cut(s) 663, 769, 1003
NcoI CCATGG 2 cut(s) 105, 265
NdeII GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
NlaIII CATG 3 cut(s) 109, 269, 528
NlaIV GGNNCC 4 cut(s) 94, 220, 771, 929
NmeAIII GCCGAG 1 cut(s) 1249
NsiI ATGCAT 1 cut(s) 423
OliI CACNNNNGTG 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 1236
PcsI WCGNNNNNNNCGW 2 cut(s) 540, 1117
PctI GAATGC 2 cut(s) 551, 576
PkrI GCNGC 4 cut(s) 197, 307, 1222, 1261
Ple19I CGATCG 1 cut(s) 534
PleI GAGTC 2 cut(s) 5, 944
PpsI GAGTC 2 cut(s) 5, 944
Ppu21I YACGTR 2 cut(s) 131, 1249
PpuMI RGGWCCY 1 cut(s) 611
PsiI TTATAA 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 611
Psp6I CCWGG 3 cut(s) 575, 601, 934
PspGI CCWGG 3 cut(s) 575, 601, 934
PspN4I GGNNCC 4 cut(s) 94, 220, 771, 929
PspPI GGNCC 3 cut(s) 193, 521, 611
PspPPI RGGWCCY 1 cut(s) 611
PvuI CGATCG 1 cut(s) 534
RsaI GTAC 3 cut(s) 31, 514, 1151
RsaNI GTAC 3 cut(s) 30, 513, 1150
RseI CAYNNNNRTG 3 cut(s) 270, 321, 882
SaqAI TTAA 3 cut(s) 162, 491, 1279
SatI GCNGC 4 cut(s) 196, 306, 1221, 1260
Sau3AI GATC 7 cut(s) 97, 383, 531, 535, 889, 1101, 1168
Sau96I GGNCC 3 cut(s) 193, 521, 611
SchI GAGTC 2 cut(s) 5, 944
ScrFI CCNGG 3 cut(s) 577, 603, 936
SduI GDGCHC 1 cut(s) 999
SfaNI GCATC 5 cut(s) 14, 509, 644, 993, 1187
Sfr274I CTCGAG 1 cut(s) 1236
SinI GGWCC 1 cut(s) 611
SlaI CTCGAG 1 cut(s) 1236
SmiMI CAYNNNNRTG 3 cut(s) 270, 321, 882
SmlI CTYRAG 1 cut(s) 1236
SmoI CTYRAG 1 cut(s) 1236
SnaBI TACGTA 1 cut(s) 1249
Sse9I AATT 4 cut(s) 249, 787, 853, 1276
SsiI CCGC 3 cut(s) 196, 372, 906
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 3 cut(s) 575, 601, 934
StyI CCWWGG 3 cut(s) 105, 189, 265
TaaI ACNGT 3 cut(s) 323, 927, 1154
TaiI ACGT 2 cut(s) 133, 1251
TaqI TCGA 3 cut(s) 534, 652, 1237
TasI AATT 4 cut(s) 249, 787, 853, 1276
TauI GCSGC 1 cut(s) 198
Tru1I TTAA 3 cut(s) 162, 491, 1279
Tru9I TTAA 3 cut(s) 162, 491, 1279
TscAI CASTG 1 cut(s) 1039
TseI GCWGC 3 cut(s) 305, 1220, 1259
TspDTI ATGAA 6 cut(s) 339, 455, 951, 1097, 1176, 1218
TspGWI ACGGA 1 cut(s) 712
TspRI CASTG 1 cut(s) 1039
VpaK11BI GGWCC 1 cut(s) 611
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 2 cut(s) 787, 853
XbaI TCTAGA 1 cut(s) 239
XhoI CTCGAG 1 cut(s) 1236
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 1 cut(s) 240
Zsp2I ATGCAT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.