Rh5AG528900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001112
Physical Location & Seq
Forward (+)
90683506 .. 90687213
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG528900.1

Sequence Viewer

Length: 594 bp
ATGACCACCAATACTGGATTTATGAATACATATGGTATGACTCCTTTTAATGTTTGTATGGATGTTTGGAAGGAGCCTTGTGATATAGTGCAGCCTACGGTTTATAATGTGGAACAGGGATTGAAAGCATCACCAGACTCTAAAATAAGTGATGGCAGATGTGGTCCTGCTGATAATGCTCTAGGATCTTTAAAAGATGCAGTTGTGGAGGAGGTTTCCTGTGAGATTGGCAGCACTGTTGTAGATAATAATGGTAAAGGCCCTGGTGCAACGTCCAGCTTCCTTGATGATCCAAAATATCCTGAAGAGATGTCAGGTGGTGAGAGCTGTCACTTACATAAGTGTTGTCCTGAGGTCAAGCCTGTAGGCAAAACAGAAGATGTGATTAAGGCTGCACCAGGTGCCTCTGTTGGTGAAAGCCTCTCTTTGGTAACTTTAATTGCAAAGGATCTGGTTGAATCTGCATCTCATAGGCTTGTTCCAAGTCATCCATACTCTGCCATTACTACCTCACAGAAGACTACATCTTCAACAAGTACATCAAGTGGTTGTATGGCTCAATATAATAATGGAAATGGTGGTACATACTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

20.63

Weight (kDa)

4.82

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 105
AccB1I GGYRCC 1 cut(s) 401
AclWI GGATC 3 cut(s) 193, 284, 456
AcuI CTGAAG 1 cut(s) 324
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 2 cut(s) 538, 583
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 3 cut(s) 124, 458, 531
AjnI CCWGG 2 cut(s) 262, 397
AluBI AGCT 2 cut(s) 279, 327
AluI AGCT 2 cut(s) 279, 327
AlwI GGATC 3 cut(s) 193, 284, 456
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 3 cut(s) 91, 231, 392
AspS9I GGNCC 2 cut(s) 164, 260
AsuHPI GGTGA 3 cut(s) 123, 332, 425
AvaII GGWCC 1 cut(s) 164
AxyI CCTNAGG 1 cut(s) 351
BanI GGYRCC 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 524
BbvI GCAGC 3 cut(s) 103, 243, 379
BccI CCATC 1 cut(s) 146
BciT130I CCWGG 2 cut(s) 264, 399
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 3 cut(s) 92, 232, 393
BlsI GCNGC 3 cut(s) 93, 233, 394
Bme1390I CCNGG 2 cut(s) 264, 399
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 164, 260
BmiI GGNNCC 2 cut(s) 75, 403
BmrFI CCNGG 2 cut(s) 264, 399
BmsI GCATC 3 cut(s) 137, 187, 473
BpiI GAAGAC 1 cut(s) 524
BsaJI CCNNGG 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 1 cut(s) 19
Bse21I CCTNAGG 1 cut(s) 351
BseBI CCWGG 2 cut(s) 264, 399
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 2 cut(s) 67, 487
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 103, 243, 379
BsgI GTGCAG 2 cut(s) 110, 378
BshFI GGCC 1 cut(s) 261
BshNI GGYRCC 1 cut(s) 401
BslI CCNNNNNNNGG 1 cut(s) 427
BsnI GGCC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 185, 289, 448
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 75, 403
BspPI GGATC 3 cut(s) 193, 284, 456
BspT107I GGYRCC 1 cut(s) 401
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 3 cut(s) 185, 289, 448
Bst2UI CCWGG 2 cut(s) 264, 399
Bst4CI ACNGT 2 cut(s) 100, 238
Bst6I CTCTTC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 401
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 2 cut(s) 67, 487
BstKTI GATC 3 cut(s) 188, 292, 451
BstMBI GATC 3 cut(s) 185, 289, 448
BstMWI GCNNNNNNNGC 2 cut(s) 176, 401
BstNI CCWGG 2 cut(s) 264, 399
BstSCI CCNGG 2 cut(s) 262, 397
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 3 cut(s) 103, 243, 379
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 2 cut(s) 185, 448
BstYI RGATCY 2 cut(s) 185, 448
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 1 cut(s) 261
BtsCI GGATG 2 cut(s) 67, 487
BtsIMutI CAGTG 1 cut(s) 234
Cfr13I GGNCC 2 cut(s) 164, 260
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 2 cut(s) 537, 582
CviQI GTAC 2 cut(s) 537, 582
DdeI CTNAG 1 cut(s) 351
DpnI GATC 3 cut(s) 187, 291, 450
DpnII GATC 3 cut(s) 185, 289, 448
DraI TTTAAA 1 cut(s) 192
DraIII CACNNNGTG 1 cut(s) 401
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 324
Eco81I CCTNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 262, 397
FalI AAGNNNNNCTT 2 cut(s) 409, 441
FauNDI CATATG 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 92, 232, 393
FokI GGATG 2 cut(s) 74, 474
Fsp4HI GCNGC 3 cut(s) 92, 232, 393
FspBI CTAG 1 cut(s) 182
GluI GCNGC 3 cut(s) 92, 232, 393
HaeIII GGCC 1 cut(s) 261
HinfI GANTC 3 cut(s) 40, 137, 458
HphI GGTGA 3 cut(s) 123, 332, 425
Hpy188III TCNNGA 2 cut(s) 302, 350
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 2 cut(s) 100, 238
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 6 cut(s) 91, 200, 269, 395, 443, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 401
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 1 cut(s) 272
Kzo9I GATC 3 cut(s) 185, 289, 448
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 3 cut(s) 103, 243, 379
LweI GCATC 3 cut(s) 137, 187, 473
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 1 cut(s) 182
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 329, 430
MalI GATC 3 cut(s) 187, 291, 450
MboI GATC 3 cut(s) 185, 289, 448
MboII GAAGA 4 cut(s) 317, 389, 519, 529
MflI RGATCY 2 cut(s) 185, 448
MluCI AATT 1 cut(s) 438
MlyI GAGTC 2 cut(s) 34, 131
MnlI CCTC 6 cut(s) 202, 205, 346, 415, 431, 520
MseI TTAA 4 cut(s) 48, 191, 387, 437
MspR9I CCNGG 2 cut(s) 264, 399
MvaI CCWGG 2 cut(s) 264, 399
MwoI GCNNNNNNNGC 2 cut(s) 176, 401
NdeI CATATG 1 cut(s) 31
NdeII GATC 3 cut(s) 185, 289, 448
NlaIV GGNNCC 2 cut(s) 75, 403
NmuCI GTSAC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 458
PkrI GCNGC 3 cut(s) 93, 233, 394
PleI GAGTC 2 cut(s) 34, 131
PpsI GAGTC 2 cut(s) 34, 131
PsiI TTATAA 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 262, 397
PspGI CCWGG 2 cut(s) 262, 397
PspN4I GGNNCC 2 cut(s) 75, 403
PspPI GGNCC 2 cut(s) 164, 260
PsuI RGATCY 2 cut(s) 185, 448
RsaI GTAC 2 cut(s) 538, 583
RsaNI GTAC 2 cut(s) 537, 582
SaqAI TTAA 4 cut(s) 48, 191, 387, 437
SatI GCNGC 3 cut(s) 92, 232, 393
Sau3AI GATC 3 cut(s) 185, 289, 448
Sau96I GGNCC 2 cut(s) 164, 260
SchI GAGTC 2 cut(s) 34, 131
ScrFI CCNGG 2 cut(s) 264, 399
SetI ASST 8 cut(s) 216, 275, 281, 319, 329, 357, 403, 512
SexAI ACCWGGT 1 cut(s) 397
SfaNI GCATC 3 cut(s) 137, 187, 473
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 1 cut(s) 164
Sse9I AATT 1 cut(s) 438
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 2 cut(s) 262, 397
TaaI ACNGT 2 cut(s) 100, 238
TaiI ACGT 1 cut(s) 275
TasI AATT 1 cut(s) 438
TatI WGTACW 1 cut(s) 536
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 4 cut(s) 48, 191, 387, 437
Tru9I TTAA 4 cut(s) 48, 191, 387, 437
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 3 cut(s) 91, 231, 392
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 1 cut(s) 38
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 164
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.