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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 32 |
| AclWI | GGATC | 2 cut(s) | 316, 419 |
| AcuI | CTGAAG | 1 cut(s) | 354 |
| AdeI | CACNNNGTG | 1 cut(s) | 218 |
| AflIII | ACRYGT | 1 cut(s) | 236 |
| AgsI | TTSAA | 5 cut(s) | 117, 164, 196, 229, 345 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 306, 338 |
| AluBI | AGCT | 2 cut(s) | 105, 338 |
| AluI | AGCT | 2 cut(s) | 105, 338 |
| Alw26I | GTCTC | 1 cut(s) | 40 |
| AlwI | GGATC | 2 cut(s) | 316, 419 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 65 |
| ApeKI | GCWGC | 2 cut(s) | 251, 521 |
| AseI | ATTAAT | 2 cut(s) | 246, 468 |
| AsuHPI | GGTGA | 3 cut(s) | 230, 338, 407 |
| BbsI | GAAGAC | 1 cut(s) | 30 |
| BbvI | GCAGC | 2 cut(s) | 238, 508 |
| BccI | CCATC | 3 cut(s) | 202, 251, 401 |
| BcoDI | GTCTC | 1 cut(s) | 40 |
| BfmI | CTRYAG | 1 cut(s) | 354 |
| BisI | GCNGC | 2 cut(s) | 252, 522 |
| BlsI | GCNGC | 2 cut(s) | 253, 523 |
| BmiI | GGNNCC | 1 cut(s) | 475 |
| BmsI | GCATC | 1 cut(s) | 500 |
| BpiI | GAAGAC | 1 cut(s) | 30 |
| Bse1I | ACTGG | 1 cut(s) | 397 |
| BseMII | CTCAG | 2 cut(s) | 72, 357 |
| BseNI | ACTGG | 1 cut(s) | 397 |
| BseRI | GAGGAG | 1 cut(s) | 97 |
| BseXI | GCAGC | 2 cut(s) | 238, 508 |
| BsmAI | GTCTC | 1 cut(s) | 40 |
| Bsp143I | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| BspCNI | CTCAG | 2 cut(s) | 71, 358 |
| BspLI | GGNNCC | 1 cut(s) | 475 |
| BspPI | GGATC | 2 cut(s) | 316, 419 |
| BsrI | ACTGG | 1 cut(s) | 397 |
| BssMI | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| Bst4CI | ACNGT | 4 cut(s) | 82, 172, 224, 463 |
| BstDEI | CTNAG | 3 cut(s) | 58, 101, 366 |
| BstKTI | GATC | 5 cut(s) | 134, 147, 311, 427, 446 |
| BstMAI | GTCTC | 1 cut(s) | 40 |
| BstMBI | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| BstNSI | RCATGY | 1 cut(s) | 240 |
| BstSFI | CTRYAG | 1 cut(s) | 354 |
| BstV1I | GCAGC | 2 cut(s) | 238, 508 |
| BstV2I | GAAGAC | 1 cut(s) | 30 |
| BstX2I | RGATCY | 2 cut(s) | 308, 424 |
| BstYI | RGATCY | 2 cut(s) | 308, 424 |
| BtsI | GCAGTG | 1 cut(s) | 486 |
| BtsIMutI | CAGTG | 1 cut(s) | 486 |
| CaiI | CAGNNNCTG | 1 cut(s) | 65 |
| CviAII | CATG | 4 cut(s) | 47, 155, 215, 237 |
| CviJI | RGCY | 4 cut(s) | 17, 105, 261, 338 |
| CviKI_1 | RGCY | 4 cut(s) | 17, 105, 261, 338 |
| DdeI | CTNAG | 3 cut(s) | 58, 101, 366 |
| DpnI | GATC | 5 cut(s) | 133, 146, 310, 426, 445 |
| DpnII | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| DraIII | CACNNNGTG | 1 cut(s) | 218 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 32 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 32 |
| Eco57I | CTGAAG | 1 cut(s) | 354 |
| FaeI | CATG | 4 cut(s) | 50, 158, 218, 240 |
| FatI | CATG | 4 cut(s) | 46, 154, 214, 236 |
| FauNDI | CATATG | 1 cut(s) | 517 |
| Fnu4HI | GCNGC | 2 cut(s) | 252, 522 |
| Fsp4HI | GCNGC | 2 cut(s) | 252, 522 |
| GluI | GCNGC | 2 cut(s) | 252, 522 |
| Hin1II | CATG | 4 cut(s) | 50, 158, 218, 240 |
| HindIII | AAGCTT | 1 cut(s) | 336 |
| HinfI | GANTC | 3 cut(s) | 62, 90, 329 |
| HphI | GGTGA | 3 cut(s) | 230, 338, 407 |
| Hpy166II | GTNNAC | 1 cut(s) | 53 |
| Hpy188I | TCNGA | 5 cut(s) | 61, 187, 313, 334, 457 |
| Hpy188III | TCNNGA | 2 cut(s) | 196, 306 |
| Hpy8I | GTNNAC | 1 cut(s) | 53 |
| HpyCH4III | ACNGT | 4 cut(s) | 82, 172, 224, 463 |
| HpyCH4V | TGCA | 4 cut(s) | 491, 524, 538, 552 |
| HpyF3I | CTNAG | 3 cut(s) | 58, 101, 366 |
| Hsp92II | CATG | 4 cut(s) | 50, 158, 218, 240 |
| Kzo9I | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| LmnI | GCTCC | 1 cut(s) | 110 |
| LpnPI | CCDG | 2 cut(s) | 291, 378 |
| Lsp1109I | GCAGC | 2 cut(s) | 238, 508 |
| LweI | GCATC | 1 cut(s) | 500 |
| MaeIII | GTNAC | 3 cut(s) | 218, 239, 371 |
| MalI | GATC | 5 cut(s) | 133, 146, 310, 426, 445 |
| MboI | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| MboII | GAAGA | 3 cut(s) | 35, 35, 333 |
| MfeI | CAATTG | 1 cut(s) | 317 |
| MflI | RGATCY | 2 cut(s) | 308, 424 |
| MluCI | AATT | 4 cut(s) | 125, 281, 317, 547 |
| MlyI | GAGTC | 1 cut(s) | 71 |
| MnlI | CCTC | 5 cut(s) | 67, 118, 183, 361, 375 |
| MseI | TTAA | 2 cut(s) | 246, 468 |
| MslI | CAYNNNNRTG | 2 cut(s) | 246, 393 |
| MunI | CAATTG | 1 cut(s) | 317 |
| NdeI | CATATG | 1 cut(s) | 517 |
| NdeII | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| NlaIII | CATG | 4 cut(s) | 50, 158, 218, 240 |
| NlaIV | GGNNCC | 1 cut(s) | 475 |
| NmuCI | GTSAC | 2 cut(s) | 218, 239 |
| NspI | RCATGY | 1 cut(s) | 240 |
| PciI | ACATGT | 1 cut(s) | 236 |
| PfeI | GAWTC | 2 cut(s) | 90, 329 |
| PflFI | GACNNNGTC | 1 cut(s) | 461 |
| PkrI | GCNGC | 2 cut(s) | 253, 523 |
| PleI | GAGTC | 1 cut(s) | 70 |
| PpsI | GAGTC | 1 cut(s) | 70 |
| PscI | ACATGT | 1 cut(s) | 236 |
| PshBI | ATTAAT | 2 cut(s) | 246, 468 |
| PspN4I | GGNNCC | 1 cut(s) | 475 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 24, 56 |
| PstNI | CAGNNNCTG | 1 cut(s) | 65 |
| PsuI | RGATCY | 2 cut(s) | 308, 424 |
| PsyI | GACNNNGTC | 1 cut(s) | 461 |
| RseI | CAYNNNNRTG | 2 cut(s) | 246, 393 |
| SaqAI | TTAA | 2 cut(s) | 246, 468 |
| SatI | GCNGC | 2 cut(s) | 252, 522 |
| Sau3AI | GATC | 5 cut(s) | 131, 144, 308, 424, 443 |
| SchI | GAGTC | 1 cut(s) | 71 |
| SetI | ASST | 4 cut(s) | 107, 194, 340, 479 |
| SfaNI | GCATC | 1 cut(s) | 500 |
| SfcI | CTRYAG | 1 cut(s) | 354 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 246, 393 |
| Sse9I | AATT | 4 cut(s) | 125, 281, 317, 547 |
| TaaI | ACNGT | 4 cut(s) | 82, 172, 224, 463 |
| TasI | AATT | 4 cut(s) | 125, 281, 317, 547 |
| TfiI | GAWTC | 2 cut(s) | 90, 329 |
| Tru1I | TTAA | 2 cut(s) | 246, 468 |
| Tru9I | TTAA | 2 cut(s) | 246, 468 |
| TscAI | CASTG | 1 cut(s) | 493 |
| TseFI | GTSAC | 2 cut(s) | 218, 239 |
| TseI | GCWGC | 2 cut(s) | 251, 521 |
| Tsp45I | GTSAC | 2 cut(s) | 218, 239 |
| TspDTI | ATGAA | 3 cut(s) | 35, 36, 216 |
| TspRI | CASTG | 1 cut(s) | 493 |
| Tth111I | GACNNNGTC | 1 cut(s) | 461 |
| VspI | ATTAAT | 2 cut(s) | 246, 468 |
| XceI | RCATGY | 1 cut(s) | 240 |