Rmu_sc0000625.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000625.1
OG0001112
Physical Location & Seq
Forward (+)
3261 .. 6957
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000625.1_g000001.1.cds

Sequence Viewer

Length: 2670 bp
atgaggaggcctctcatcggctctggatctctatggatcggagatttatcgagctctgaagtgactactgaattagatcttgaagaaagttcaaaggcggcggcagtggatttgaaggagcttagttgggattgggattttagggttagattttacatgatccatgatcgttctgctgttatggatagagggaaccccatcacaactcatttttgtgcttgccctgggggtgtacccatcaatctggcgcggagtaggaagcaagcaagaacaacatcttgcaaaactgctggcggcaggcaaacagtggatgggattagatctatttcccaaagcatgatgaatacagttcgagtagaacttacaaattctattagtccagatttgagttggaaggtttctaagggttacagaagtgcaccaaggcgaaggaaacaggttgataaaggaccaaaattgggtgcggagccagcaaataaaagtactaaaagttcatctgaaacaactgtttctgaatcagagaagcttggagtggctgtacttggaagacggttcagcgacaaaattgaacatgtgccgattaagaaaagaagatttatggtccgttctccgtcaccaccccctcgcttatcttctcagttacctgaaaacaatcaaccaggtgtgggtgatctacgtgcttcaggtcagaagtcatgttctaaatcaattgccaaaaagcatacaacaaggagtgatacttcttcggtgactagttttactcatagtgctgttactgttaacgagagtttaactgagccggcaaatgaaaagcctggttatggggaggacttctctggtattgagatattggctgctgctgcctgcaacaacagcatacatgatgatataggtgaagaattatcacgagaagggacgaatgcatcaacctctgcgaggcccttggcgaaaactgttgcatccttttcatcaacgccaatgataactgattcagagatatcagaaggaagagatgcatcattggaaacaattcattattcgcctaaagatgtagtacatgaagataaaatagagaactcctttgttgaggatagcttggttaaaactaatactggtgacggagatgaccaagcagtagcatcttcaaaggatgttaggttgcactgggatctaaatgtttgtatggatgcttgggaggagccttgtgatacggtgatggttgatcctcaagcaaatgctgtagacgacatctgtattgacagacagagcgaaaagcagccagtttcacaagatggtgatgcacagggatccgtagaagctaagaatcctgaactgcttcctgtggttgatgatgtggaacagggattgaaagcatccccggactctgaaataagttatggtaaaagtggcctggctgataatgctctaggatcttcaaaagatgcaggtgtggttacagacaattgcaatcaggatgtcagcatggatgcatgtggtgataattcctcctgtgttcgctttgacaatattgcaggcacacctactgtgcctttagaaaagaatggaacaccacctgccagtattggtgcacaactgggtgacaccaactgttcaggaaagatccagctcgaaaaccctgctataacttgtgtgcctgttgtggaaggggtttcttgtgagatgggcagcactgttgtagatgatgatggtaagggctctgctgcaacatccagcttccatgatgatccaaaatccccagaagagatgacagcggtcgagagctgtcacttacagaagagttgtccagaggtcaagcctgtatgcaaaacagaagatgtgattaagcctgcatcaggtgcatctgttggtgaaggcctctctttggtgactttaattgtaaaggatcaggttgaagctgcatctcagattcatactgttaattctctacaaaatgttgggtctctagaagtaatgcctaaatcttctgtgagtgccacaaaggatcctccagctgatgctggtccctcccgtaacgagcaatgccactatggtgatggtactgcaggaagtaaagtcactacagtagattcttctggtgtcaacagtcacttggatgataaggatcacatggttgccaacaacaaccctacggaacaggaggtcggatatgattctcaatatgaggacggggagttgagggaatcagatgtaccttattgggaggagaatgaatttgacgaagttgaacgtgttgattatggttctgatacatgtgacactgatgctgctgatggctctgtatcaggaaaagtggaatgcagtgaattttgcggggtagaatctaggaacattaacatgaacgtacaggttgtgcgaggattgagcctaggttctgataatatgtgtggaaaagttgaacatagtaccactggaaatgctttgagacaatcttcagtgggttcaaagacgagaacttctggatctgaacaattgcccggtgattctgaagcttcttcaaacagaactgcaggagcaactgagggttgcacaataagaaaacatactggtgattgctttgatggccttaatggtaaagatcctgccaaagtggatggatcaatgacatctgataccttgaataggatgggaacttcatctgggaggaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

889

Amino Acids

94.71

Weight (kDa)

4.8

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1436, 1582
Acc36I ACCTGC 2 cut(s) 1436, 1582
AccI GTMKAC 1 cut(s) 1242
AccII CGCG 1 cut(s) 250
AciI CCGC 7 cut(s) 98, 101, 250, 294, 464, 1772, 2331
AcsI RAATTY 3 cut(s) 367, 2231, 2324
AcuI CTGAAG 4 cut(s) 78, 666, 2436, 2526
AfaI GTAC 8 cut(s) 234, 484, 540, 1056, 2059, 2211, 2364, 2425
AfiI CCNNNNNNNGG 8 cut(s) 17, 458, 665, 821, 936, 1853, 1882, 2640
AflIII ACRYGT 3 cut(s) 571, 2248, 2270
AhlI ACTAGT 1 cut(s) 752
AjnI CCWGG 4 cut(s) 223, 658, 814, 1410
AleI CACNNNNGTG 1 cut(s) 2049
Alw21I GWGCWC 3 cut(s) 56, 421, 1591
Alw26I GTCTC 2 cut(s) 1965, 2437
Alw44I GTGCAC 2 cut(s) 417, 1587
AoxI GGCC 5 cut(s) 8, 938, 1408, 1873, 2581
ApaLI GTGCAC 2 cut(s) 417, 1587
ApeKI GCWGC 8 cut(s) 854, 857, 860, 1276, 1686, 1721, 1916, 2285
ApoI RAATTY 3 cut(s) 367, 2231, 2324
Asp700I GAANNNNTTC 2 cut(s) 1029, 1335
AspA2I CCTAGG 1 cut(s) 2386
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 4 cut(s) 449, 601, 939, 2021
AsuC2I CCSGG 2 cut(s) 1379, 2496
AvaII GGWCC 3 cut(s) 449, 601, 2021
AvrII CCTAGG 1 cut(s) 2386
BaeGI GKGCMC 2 cut(s) 421, 1591
BamHI GGATCC 2 cut(s) 1307, 2002
BanII GRGCYC 2 cut(s) 56, 1718
BauI CACGAG 1 cut(s) 906
BbsI GAAGAC 1 cut(s) 553
Bbv12I GWGCWC 3 cut(s) 56, 421, 1591
BbvI GCAGC 8 cut(s) 841, 844, 847, 1288, 1698, 1708, 1903, 2272
BcgI CGANNNNNNTGC 2 cut(s) 1619, 1653
BciT130I CCWGG 4 cut(s) 225, 660, 816, 1412
BcnI CCSGG 2 cut(s) 1379, 2496
BcoDI GTCTC 2 cut(s) 1965, 2437
BcuI ACTAGT 1 cut(s) 752
BfaI CTAG 5 cut(s) 753, 1427, 1964, 2343, 2387
BfmI CTRYAG 4 cut(s) 1239, 2061, 2079, 2526
BfuAI ACCTGC 2 cut(s) 1436, 1582
BglII AGATCT 2 cut(s) 76, 320
BlnI CCTAGG 1 cut(s) 2386
BmcAI AGTACT 1 cut(s) 484
Bme1390I CCNGG 6 cut(s) 225, 660, 816, 1379, 1412, 2496
Bme18I GGWCC 3 cut(s) 449, 601, 2021
BmgT120I GGNCC 4 cut(s) 449, 601, 939, 2021
BmiI GGNNCC 6 cut(s) 194, 468, 1200, 1309, 2004, 2023
BmrFI CCNGG 6 cut(s) 225, 660, 816, 1379, 1412, 2496
BmrI ACTGGG 2 cut(s) 1174, 1604
BmuI ACTGGG 2 cut(s) 1174, 1604
BoxI GACNNNNGTC 1 cut(s) 1772
BpiI GAAGAC 1 cut(s) 553
BplI GAGNNNNNCTC 3 cut(s) 27, 818, 850
BpmI CTGGAG 1 cut(s) 1992
BpuEI CTTGAG 1 cut(s) 1212
BpuMI CCSGG 2 cut(s) 1379, 2496
BsaAI YACGTR 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 2121
BsaI GGTCTC 1 cut(s) 1965
BsaJI CCNNGG 6 cut(s) 223, 224, 422, 942, 1377, 2386
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 8 cut(s) 17, 458, 665, 821, 936, 1853, 1882, 2640
Bse118I RCCGGY 1 cut(s) 799
Bse1I ACTGG 7 cut(s) 1117, 1169, 1280, 1578, 1599, 2434, 2569
Bse3DI GCAATG 1 cut(s) 2045
Bse8I GATNNNNATC 1 cut(s) 2121
BseBI CCWGG 4 cut(s) 225, 660, 816, 1412
BseDI CCNNGG 6 cut(s) 223, 224, 422, 942, 1377, 2386
BseJI GATNNNNATC 1 cut(s) 2121
BseLI CCNNNNNNNGG 8 cut(s) 17, 458, 665, 821, 936, 1853, 1882, 2640
BseMI GCAATG 1 cut(s) 2045
BseMII CTCAG 4 cut(s) 650, 786, 1937, 2529
BseNI ACTGG 7 cut(s) 1117, 1169, 1280, 1578, 1599, 2434, 2569
BseRI GAGGAG 3 cut(s) 19, 1211, 2237
BseSI GKGCMC 2 cut(s) 421, 1591
BseXI GCAGC 8 cut(s) 841, 844, 847, 1288, 1698, 1708, 1903, 2272
Bsh1236I CGCG 1 cut(s) 250
Bsh1285I CGRYCG 1 cut(s) 1776
BshFI GGCC 5 cut(s) 10, 940, 1410, 1875, 2583
BsiEI CGRYCG 1 cut(s) 1776
BsiHKAI GWGCWC 3 cut(s) 56, 421, 1591
BsiSI CCGG 3 cut(s) 800, 1379, 2496
BslFI GGGAC 2 cut(s) 928, 2007
BslI CCNNNNNNNGG 8 cut(s) 17, 458, 665, 821, 936, 1853, 1882, 2640
BsmAI GTCTC 2 cut(s) 1965, 2437
BsmFI GGGAC 2 cut(s) 928, 2007
BsmI GAATGC 2 cut(s) 925, 2321
BsnI GGCC 5 cut(s) 10, 940, 1410, 1875, 2583
Bso31I GGTCTC 1 cut(s) 1965
Bsp1286I GDGCHC 4 cut(s) 56, 421, 1591, 1718
BspACI CCGC 7 cut(s) 98, 101, 250, 294, 464, 1772, 2331
BspANI GGCC 5 cut(s) 10, 940, 1410, 1875, 2583
BspCNI CTCAG 4 cut(s) 649, 787, 1936, 2530
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 6 cut(s) 194, 468, 1200, 1309, 2004, 2023
BspMAI CTGCAG 2 cut(s) 2065, 2530
BspMI ACCTGC 2 cut(s) 1436, 1582
BspTNI GGTCTC 1 cut(s) 1965
BsrDI GCAATG 1 cut(s) 2045
BsrFI RCCGGY 1 cut(s) 799
BsrI ACTGG 7 cut(s) 1117, 1169, 1280, 1578, 1599, 2434, 2569
BssAI RCCGGY 1 cut(s) 799
BssECI CCNNGG 6 cut(s) 223, 224, 422, 942, 1377, 2386
BssSI CACGAG 1 cut(s) 906
BssT1I CCWWGG 3 cut(s) 422, 942, 2386
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 4 cut(s) 225, 660, 816, 1412
Bst6I CTCTTC 3 cut(s) 1003, 1755, 1790
BstAPI GCANNNNNTGC 1 cut(s) 1856
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 9 cut(s) 220, 264, 292, 299, 471, 801, 865, 1534, 1848
BstDEI CTNAG 7 cut(s) 122, 402, 636, 795, 1320, 1923, 2538
BstENI CCTNNNNNAGG 3 cut(s) 934, 1851, 2638
BstFNI CGCG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 250
BstMAI GTCTC 2 cut(s) 1965, 2437
BstMCI CGRYCG 1 cut(s) 1776
BstMWI GCNNNNNNNGC 6 cut(s) 470, 860, 873, 1421, 1856, 2580
BstNI CCWGG 4 cut(s) 225, 660, 816, 1412
BstNSI RCATGY 3 cut(s) 575, 1494, 2274
BstPAI GACNNNNGTC 1 cut(s) 1772
BstSCI CCNGG 6 cut(s) 223, 658, 814, 1377, 1410, 2494
BstSFI CTRYAG 4 cut(s) 1239, 2061, 2079, 2526
BstSLI GKGCMC 2 cut(s) 421, 1591
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 8 cut(s) 841, 844, 847, 1288, 1698, 1708, 1903, 2272
BstV2I GAAGAC 1 cut(s) 553
BstXI CCANNNNNNTGG 1 cut(s) 244
BsuRI GGCC 5 cut(s) 10, 940, 1410, 1875, 2583
BtsI GCAGTG 2 cut(s) 111, 2326
BtsIMutI CAGTG 8 cut(s) 111, 312, 1162, 1689, 2277, 2326, 2427, 2460
BveI ACCTGC 2 cut(s) 1436, 1582
Cac8I GCNNGC 9 cut(s) 220, 264, 292, 299, 471, 801, 865, 1534, 1848
CfoI GCGC 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 799
Cfr13I GGNCC 4 cut(s) 449, 601, 939, 2021
CsiI ACCWGGT 1 cut(s) 658
Csp6I GTAC 8 cut(s) 233, 483, 539, 1055, 2058, 2210, 2363, 2424
CviQI GTAC 8 cut(s) 233, 483, 539, 1055, 2058, 2210, 2363, 2424
DdeI CTNAG 7 cut(s) 122, 402, 636, 795, 1320, 1923, 2538
Eam1104I CTCTTC 3 cut(s) 1003, 1755, 1790
EarI CTCTTC 3 cut(s) 1003, 1755, 1790
Ecl136II GAGCTC 1 cut(s) 54
Eco130I CCWWGG 3 cut(s) 422, 942, 2386
Eco147I AGGCCT 2 cut(s) 10, 1875
Eco24I GRGCYC 2 cut(s) 56, 1718
Eco31I GGTCTC 1 cut(s) 1965
Eco32I GATATC 1 cut(s) 999
Eco47I GGWCC 3 cut(s) 449, 601, 2021
Eco53kI GAGCTC 1 cut(s) 54
Eco57I CTGAAG 4 cut(s) 78, 666, 2436, 2526
EcoICRI GAGCTC 1 cut(s) 54
EcoNI CCTNNNNNAGG 3 cut(s) 934, 1851, 2638
EcoO109I RGGNCCY 1 cut(s) 939
EcoRII CCWGG 4 cut(s) 223, 658, 814, 1410
EcoRV GATATC 1 cut(s) 999
EcoT14I CCWWGG 3 cut(s) 422, 942, 2386
EcoT22I ATGCAT 3 cut(s) 925, 1018, 1492
EcoT38I GRGCYC 2 cut(s) 56, 1718
ErhI CCWWGG 3 cut(s) 422, 942, 2386
FaqI GGGAC 2 cut(s) 928, 2007
FauI CCCGC 1 cut(s) 2324
FblI GTMKAC 1 cut(s) 1242
FriOI GRGCYC 2 cut(s) 56, 1718
FspBI CTAG 5 cut(s) 753, 1427, 1964, 2343, 2387
GlaI GCGC 1 cut(s) 249
GsuI CTGGAG 1 cut(s) 1992
HaeIII GGCC 5 cut(s) 10, 940, 1410, 1875, 2583
HapII CCGG 3 cut(s) 800, 1379, 2496
HhaI GCGC 1 cut(s) 250
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 2 cut(s) 781, 2101
HindII GTYRAC 2 cut(s) 781, 2101
HindIII AAGCTT 2 cut(s) 524, 2508
HpaI GTTAAC 1 cut(s) 781
HpaII CCGG 3 cut(s) 800, 1379, 2496
Hpy166II GTNNAC 6 cut(s) 233, 419, 781, 1243, 1589, 2101
Hpy8I GTNNAC 6 cut(s) 233, 419, 781, 1243, 1589, 2101
HpyAV CCTTC 7 cut(s) 109, 388, 423, 905, 998, 1658, 1865
HpyCH4IV ACGT 3 cut(s) 676, 2248, 2361
HpyF10VI GCNNNNNNNGC 6 cut(s) 470, 860, 873, 1421, 1856, 2580
HpyF3I CTNAG 7 cut(s) 122, 402, 636, 795, 1320, 1923, 2538
HpySE526I ACGT 3 cut(s) 676, 2248, 2361
HspAI GCGC 1 cut(s) 248
KroI GCCGGC 1 cut(s) 799
KroNI GCCGGC 1 cut(s) 801
KspAI GTTAAC 1 cut(s) 781
LmnI GCTCC 4 cut(s) 118, 466, 1198, 2531
Lsp1109I GCAGC 8 cut(s) 841, 844, 847, 1288, 1698, 1708, 1903, 2272
MabI ACCWGGT 1 cut(s) 658
MaeI CTAG 5 cut(s) 753, 1427, 1964, 2343, 2387
MaeII ACGT 3 cut(s) 676, 2248, 2361
MfeI CAATTG 3 cut(s) 708, 1462, 2489
MhlI GDGCHC 4 cut(s) 56, 421, 1591, 1718
MlyI GAGTC 1 cut(s) 1376
MmeI TCCRAC 2 cut(s) 371, 2143
Mph1103I ATGCAT 3 cut(s) 925, 1018, 1492
MroNI GCCGGC 1 cut(s) 799
MroXI GAANNNNTTC 2 cut(s) 1029, 1335
MseI TTAA 9 cut(s) 582, 780, 791, 1101, 1842, 1892, 1938, 2352, 2586
MslI CAYNNNNRTG 5 cut(s) 213, 2049, 2112, 2354, 2565
MspA1I CMGCKG 2 cut(s) 1772, 2012
MspI CCGG 3 cut(s) 800, 1379, 2496
MspR9I CCNGG 6 cut(s) 225, 660, 816, 1379, 1412, 2496
MunI CAATTG 3 cut(s) 708, 1462, 2489
Mva1269I GAATGC 2 cut(s) 925, 2321
MvaI CCWGG 4 cut(s) 225, 660, 816, 1412
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 6 cut(s) 470, 860, 873, 1421, 1856, 2580
NaeI GCCGGC 1 cut(s) 801
NciI CCSGG 2 cut(s) 1379, 2496
NgoMIV GCCGGC 1 cut(s) 799
NlaIV GGNNCC 6 cut(s) 194, 468, 1200, 1309, 2004, 2023
NsiI ATGCAT 3 cut(s) 925, 1018, 1492
NspI RCATGY 3 cut(s) 575, 1494, 2274
OliI CACNNNNGTG 1 cut(s) 2049
PaqCI CACCTGC 2 cut(s) 1436, 1582
PasI CCCWGGG 1 cut(s) 224
PceI AGGCCT 2 cut(s) 10, 1875
PciI ACATGT 2 cut(s) 571, 2270
PctI GAATGC 2 cut(s) 925, 2321
PdiI GCCGGC 1 cut(s) 801
PdmI GAANNNNTTC 2 cut(s) 1029, 1335
PfeI GAWTC 9 cut(s) 515, 989, 1324, 1927, 2087, 2171, 2201, 2339, 2501
PleI GAGTC 1 cut(s) 1376
PpsI GAGTC 1 cut(s) 1376
Ppu21I YACGTR 1 cut(s) 677
PscI ACATGT 2 cut(s) 571, 2270
PshAI GACNNNNGTC 1 cut(s) 1772
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 4 cut(s) 223, 658, 814, 1410
PspGI CCWGG 4 cut(s) 223, 658, 814, 1410
PspN4I GGNNCC 6 cut(s) 194, 468, 1200, 1309, 2004, 2023
PspPI GGNCC 4 cut(s) 449, 601, 939, 2021
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PstI CTGCAG 2 cut(s) 2065, 2530
PvuII CAGCTG 1 cut(s) 2012
RsaI GTAC 8 cut(s) 234, 484, 540, 1056, 2059, 2211, 2364, 2425
RsaNI GTAC 8 cut(s) 233, 483, 539, 1055, 2058, 2210, 2363, 2424
RseI CAYNNNNRTG 5 cut(s) 213, 2049, 2112, 2354, 2565
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 9 cut(s) 582, 780, 791, 1101, 1842, 1892, 1938, 2352, 2586
Sau96I GGNCC 4 cut(s) 449, 601, 939, 2021
ScaI AGTACT 1 cut(s) 484
SchI GAGTC 1 cut(s) 1376
ScrFI CCNGG 6 cut(s) 225, 660, 816, 1379, 1412, 2496
SduI GDGCHC 4 cut(s) 56, 421, 1591, 1718
SexAI ACCWGGT 1 cut(s) 658
SfcI CTRYAG 4 cut(s) 1239, 2061, 2079, 2526
SinI GGWCC 3 cut(s) 449, 601, 2021
SmiMI CAYNNNNRTG 5 cut(s) 213, 2049, 2112, 2354, 2565
SmlI CTYRAG 1 cut(s) 1227
SmoI CTYRAG 1 cut(s) 1227
SpeI ACTAGT 1 cut(s) 752
SseBI AGGCCT 2 cut(s) 10, 1875
SsiI CCGC 7 cut(s) 98, 101, 250, 294, 464, 1772, 2331
SspI AATATT 1 cut(s) 1528
SspMI CTAG 5 cut(s) 753, 1427, 1964, 2343, 2387
SstI GAGCTC 1 cut(s) 56
StuI AGGCCT 2 cut(s) 10, 1875
StyD4I CCNGG 6 cut(s) 223, 658, 814, 1377, 1410, 2494
StyI CCWWGG 3 cut(s) 422, 942, 2386
TaiI ACGT 3 cut(s) 679, 2251, 2364
TaqI TCGA 4 cut(s) 50, 352, 1629, 1776
TatI WGTACW 3 cut(s) 482, 538, 1054
TauI GCSGC 3 cut(s) 101, 104, 297
TfiI GAWTC 9 cut(s) 515, 989, 1324, 1927, 2087, 2171, 2201, 2339, 2501
Tru1I TTAA 9 cut(s) 582, 780, 791, 1101, 1842, 1892, 1938, 2352, 2586
Tru9I TTAA 9 cut(s) 582, 780, 791, 1101, 1842, 1892, 1938, 2352, 2586
TscAI CASTG 8 cut(s) 111, 312, 1169, 1696, 2284, 2326, 2434, 2460
TseI GCWGC 8 cut(s) 854, 857, 860, 1276, 1686, 1721, 1916, 2285
TspGWI ACGGA 5 cut(s) 593, 600, 1134, 1300, 2165
TspRI CASTG 8 cut(s) 111, 312, 1169, 1696, 2284, 2326, 2434, 2460
VneI GTGCAC 2 cut(s) 417, 1587
VpaK11BI GGWCC 3 cut(s) 449, 601, 2021
XagI CCTNNNNNAGG 3 cut(s) 934, 1851, 2638
XapI RAATTY 3 cut(s) 367, 2231, 2324
XbaI TCTAGA 1 cut(s) 1963
XceI RCATGY 3 cut(s) 575, 1494, 2274
XcmI CCANNNNNNNNNTGG 2 cut(s) 387, 2051
XmaJI CCTAGG 1 cut(s) 2386
XmiI GTMKAC 1 cut(s) 1242
XmnI GAANNNNTTC 2 cut(s) 1029, 1335
XspI CTAG 5 cut(s) 753, 1427, 1964, 2343, 2387
ZrmI AGTACT 1 cut(s) 484
Zsp2I ATGCAT 3 cut(s) 925, 1018, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.