Rmu_sc0023221.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023221.1
OG0001112
Physical Location & Seq
Reverse (-)
971 .. 3388
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023221.1_g000001.1.cds

Sequence Viewer

Length: 2184 bp
atggttgccaacaacaaccctacggaacaggaggtcggatatgattctcaatatgaggacggggagttgagggaatcagatgtaccttattgggaggagaatgaatttgacgaagttgaacgtgttgattatggttctgatacatgtgacactgatgctgctgatggctctgtatcaggaaaagtggaatgcagtgaattttgcggggtagaatctaggaacattaacatgaacgtacaggttgtgcgaggattgagcctaggttctgataatatgtgtggaaaagttgaacatagtaccactggaaatgctttgagacaatcttcagtgggttcaaagacgagaacttctggatctgaacaattgcccggtgattctgaagcttcttcaaacagaactgcaggagcaactgagggttgcacaataagaaaacatactggtgattgctttgatggccttaatggtaaagatcctgccaaagtggatggatcaatgacatctgataccttgaataggatgggaacttcatctgggaggaggtatgactcttttgatttctccactcactctgaggaggtcggttctgattattccatgggaaaggaaagatctgattcacgcatgcttggtaagagtttgggaggtgttcgttttgcttctaggagttattgggattccaaacgtcgtggatcaccatcttatcgtggttcctttggttctagccgccctagaccaagaagtggtatcgagagtgatgaatatacaatggatccagatgatacattttcagaagcggcagaggttgcaggagttcacaatcgtgttcgcagacaagctatgagttataattcaaatcgttcttatcagcccacatttagaagaagatccccagatacacatagggggatgatacctatgagggatataagtcctgataggaggagaagatatccacaaggggttggtagaggagagaaccaagcgcatctccccctggcagaggaccttattatggtagacccaagttccagtctagaggcagaagtcgatctcctcttgcatgggaacggaatgaagagtttgggaggtgttcgttttgcttctaggagttattgggattccaaacgtcgtggatcaccatcttatcgtggttcctttggttctagccgccctagaccaagaagtggtatcgagagtgatgaatatgcaatggatccagatgatacattttcagaagcggcagaggttgcaggagttcacaatcgtgttcgtagacaagctatgagttataattcaaatcgttcttatcagcccacatttagaagaagatccccagatacacatagggggatgatacctatgagggatataagtcctgataggaggagaagatatccacaaggggttggtagaggtatgcgggattaccacaggcctattcccaacgagctcaatgacagttcttatgatgggccacgtcgtatgaccaggagagaaccaagcgcatctccccccggcaggggaccttattatggtagacccaagttccagtctagaggcagaagtcgatctcctcttgcatgggaacggaatgaggtttcaagacatcgtggtagcaggtcccctgattatagatttgatggtaaaatggagagggggagagtgccctttcagaagaacagttttggagcaaagtatgaagcagggttcttttccccaccaaaacgtcggttttccccacaacacaattcaagatggtttgatgatcgcaatggtgctggagatcataatttcagaggcaggagaggaggtggaaggagattccaaccgagccaaaggtttgattcagtgcgttctcggagagtggattcagatgattattttgaacccattgttcgtccaagattctcagagttgaacagtggtggtagggaatgtagatttgagggcagtgatgaagatagaaggaagcctgaaggaagatttcaaattgttcatcgtgtgcggcgttatgattctgaaggtgttgtgcgtcagttccgttacgatgaagaagatcggtttgcatcccgcaatacacagaactttgatgactgtgataataggggcgctgatcgacggcctcgagatgtttacctaggagagcagcagcagatagagaacagaagggtaactaagcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

727

Amino Acids

82.88

Weight (kDa)

9.39

Isoelectric Point (pI)

71.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 846, 1290
Acc36I ACCTGC 1 cut(s) 1608
AccB7I CCANNNNNTGG 2 cut(s) 740, 1184
AccI GTMKAC 3 cut(s) 1017, 1273, 1537
AciI CCGC 8 cut(s) 204, 724, 794, 1168, 1238, 1420, 2005, 2071
AcsI RAATTY 2 cut(s) 104, 197
AcuI CTGAAG 4 cut(s) 309, 399, 1995, 2040
AfaI GTAC 3 cut(s) 84, 237, 298
AfiI CCNNNNNNNGG 9 cut(s) 513, 740, 1000, 1012, 1184, 1518, 1519, 1520, 1532
AflIII ACRYGT 2 cut(s) 121, 143
AjiI CACGTC 1 cut(s) 1478
AjnI CCWGG 2 cut(s) 993, 1487
AleI CACNNNNGTG 2 cut(s) 819, 1263
AluBI AGCT 4 cut(s) 383, 836, 1280, 1450
AluI AGCT 4 cut(s) 383, 836, 1280, 1450
Alw21I GWGCWC 1 cut(s) 1452
Alw26I GTCTC 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 2124
AoxI GGCC 4 cut(s) 454, 1433, 1472, 2120
ApeKI GCWGC 3 cut(s) 158, 2146, 2149
ApoI RAATTY 2 cut(s) 104, 197
AspA2I CCTAGG 2 cut(s) 259, 2137
AspLEI GCGC 3 cut(s) 985, 1505, 2111
AspS9I GGNCC 4 cut(s) 1003, 1472, 1523, 1620
AsuC2I CCSGG 2 cut(s) 369, 1515
AsuHPI GGTGA 4 cut(s) 383, 452, 684, 1128
AvaI CYCGRG 1 cut(s) 2124
AvaII GGWCC 3 cut(s) 1003, 1523, 1620
AvrII CCTAGG 2 cut(s) 259, 2137
BaeGI GKGCMC 1 cut(s) 1668
BamHI GGATCC 2 cut(s) 769, 1213
BanII GRGCYC 1 cut(s) 1452
Bbv12I GWGCWC 1 cut(s) 1452
BbvI GCAGC 3 cut(s) 145, 2158, 2161
BccI CCATC 9 cut(s) 158, 446, 479, 511, 703, 1147, 1463, 1634, 1749
BceAI ACGGC 1 cut(s) 2135
BciT130I CCWGG 2 cut(s) 995, 1489
BcnI CCSGG 2 cut(s) 369, 1515
BcoDI GTCTC 1 cut(s) 310
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 2112
BfuAI ACCTGC 1 cut(s) 1608
BglII AGATCT 1 cut(s) 608
BisI GCNGC 8 cut(s) 159, 724, 795, 1168, 1239, 2006, 2147, 2150
BlnI CCTAGG 2 cut(s) 259, 2137
BlsI GCNGC 8 cut(s) 160, 725, 796, 1169, 1240, 2007, 2148, 2151
Bme1390I CCNGG 4 cut(s) 369, 995, 1489, 1515
Bme18I GGWCC 3 cut(s) 1003, 1523, 1620
BmeT110I CYCGRG 1 cut(s) 2124
BmgBI CACGTC 1 cut(s) 1478
BmgT120I GGNCC 4 cut(s) 1003, 1472, 1523, 1620
BmiI GGNNCC 6 cut(s) 709, 771, 1153, 1215, 1524, 1622
BmrFI CCNGG 4 cut(s) 369, 995, 1489, 1515
BmsI GCATC 4 cut(s) 145, 994, 1514, 2075
BpmI CTGGAG 1 cut(s) 1800
BpuMI CCSGG 2 cut(s) 369, 1515
BsaBI GATNNNNATC 2 cut(s) 694, 1138
BsaJI CCNNGG 5 cut(s) 259, 594, 993, 1513, 2137
BsaXI ACNNNNNCTCC 2 cut(s) 1795, 1825
Bsc4I CCNNNNNNNGG 9 cut(s) 513, 740, 1000, 1012, 1184, 1518, 1519, 1520, 1532
Bse1I ACTGG 4 cut(s) 307, 442, 1029, 1549
Bse3DI GCAATG 2 cut(s) 1215, 1777
Bse8I GATNNNNATC 2 cut(s) 694, 1138
BseBI CCWGG 2 cut(s) 995, 1489
BseDI CCNNGG 5 cut(s) 259, 594, 993, 1513, 2137
BseGI GGATG 5 cut(s) 490, 522, 912, 1356, 2066
BseJI GATNNNNATC 2 cut(s) 694, 1138
BseLI CCNNNNNNNGG 9 cut(s) 513, 740, 1000, 1012, 1184, 1518, 1519, 1520, 1532
BseMI GCAATG 2 cut(s) 1215, 1777
BseMII CTCAG 3 cut(s) 402, 561, 1923
BseNI ACTGG 4 cut(s) 307, 442, 1029, 1549
BseRI GAGGAG 9 cut(s) 110, 550, 587, 955, 984, 1043, 1399, 1563, 1821
BseSI GKGCMC 1 cut(s) 1668
BseXI GCAGC 3 cut(s) 145, 2158, 2161
BshFI GGCC 4 cut(s) 456, 1435, 1474, 2122
BsiHKAI GWGCWC 1 cut(s) 1452
BsiHKCI CYCGRG 1 cut(s) 2124
BsiSI CCGG 2 cut(s) 369, 1515
BslFI GGGAC 2 cut(s) 1536, 1606
BslI CCNNNNNNNGG 9 cut(s) 513, 740, 1000, 1012, 1184, 1518, 1519, 1520, 1532
BsmAI GTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 1536, 1606
BsmI GAATGC 1 cut(s) 194
BsnI GGCC 4 cut(s) 456, 1435, 1474, 2122
BsoBI CYCGRG 1 cut(s) 2124
Bsp1286I GDGCHC 2 cut(s) 1452, 1668
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 8 cut(s) 204, 724, 794, 1168, 1238, 1420, 2005, 2071
BspANI GGCC 4 cut(s) 456, 1435, 1474, 2122
BspCNI CTCAG 3 cut(s) 403, 562, 1922
BspLI GGNNCC 6 cut(s) 709, 771, 1153, 1215, 1524, 1622
BspMAI CTGCAG 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 1608
BsrDI GCAATG 2 cut(s) 1215, 1777
BsrI ACTGG 4 cut(s) 307, 442, 1029, 1549
BssECI CCNNGG 5 cut(s) 259, 594, 993, 1513, 2137
BssT1I CCWWGG 3 cut(s) 259, 594, 2137
Bst2UI CCWGG 2 cut(s) 995, 1489
Bst4CI ACNGT 4 cut(s) 1460, 1682, 1922, 2096
Bst6I CTCTTC 1 cut(s) 1070
BstAPI GCANNNNNTGC 2 cut(s) 803, 1247
BstC8I GCNNGC 1 cut(s) 623
BstDEI CTNAG 4 cut(s) 411, 570, 1909, 2175
BstDSI CCRYGG 1 cut(s) 594
BstENI CCTNNNNNAGG 2 cut(s) 511, 998
BstF5I GGATG 5 cut(s) 490, 522, 912, 1356, 2066
BstH2I RGCGCY 1 cut(s) 2112
BstHHI GCGC 3 cut(s) 985, 1505, 2111
BstMAI GTCTC 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 453, 803, 1247
BstNI CCWGG 2 cut(s) 995, 1489
BstNSI RCATGY 2 cut(s) 147, 625
BstSCI CCNGG 4 cut(s) 367, 993, 1487, 1513
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 1668
BstV1I GCAGC 3 cut(s) 145, 2158, 2161
BstX2I RGATCY 7 cut(s) 353, 469, 608, 769, 884, 1213, 1328
BstYI RGATCY 7 cut(s) 353, 469, 608, 769, 884, 1213, 1328
BsuRI GGCC 4 cut(s) 456, 1435, 1474, 2122
BtgI CCRYGG 1 cut(s) 594
BtrI CACGTC 1 cut(s) 1478
BtsCI GGATG 5 cut(s) 490, 522, 912, 1356, 2066
BtsI GCAGTG 2 cut(s) 199, 1957
BtsIMutI CAGTG 7 cut(s) 150, 199, 300, 333, 1854, 1927, 1957
BveI ACCTGC 1 cut(s) 1608
Cac8I GCNNGC 1 cut(s) 623
CfoI GCGC 3 cut(s) 985, 1505, 2111
Cfr13I GGNCC 4 cut(s) 1003, 1472, 1523, 1620
CseI GACGC 1 cut(s) 2021
Csp6I GTAC 3 cut(s) 83, 236, 297
CspCI CAANNNNNGTGG 4 cut(s) 667, 702, 1111, 1146
CviAII CATG 6 cut(s) 144, 229, 595, 622, 1061, 1581
CviQI GTAC 3 cut(s) 83, 236, 297
DdeI CTNAG 4 cut(s) 411, 570, 1909, 2175
Eam1104I CTCTTC 1 cut(s) 1070
EarI CTCTTC 1 cut(s) 1070
Ecl136II GAGCTC 1 cut(s) 1450
Eco130I CCWWGG 3 cut(s) 259, 594, 2137
Eco147I AGGCCT 1 cut(s) 1435
Eco24I GRGCYC 1 cut(s) 1452
Eco32I GATATC 2 cut(s) 950, 1394
Eco47I GGWCC 3 cut(s) 1003, 1523, 1620
Eco53kI GAGCTC 1 cut(s) 1450
Eco57I CTGAAG 4 cut(s) 309, 399, 1995, 2040
Eco88I CYCGRG 1 cut(s) 2124
EcoICRI GAGCTC 1 cut(s) 1450
EcoNI CCTNNNNNAGG 2 cut(s) 511, 998
EcoO109I RGGNCCY 3 cut(s) 1003, 1523, 1620
EcoRII CCWGG 2 cut(s) 993, 1487
EcoRV GATATC 2 cut(s) 950, 1394
EcoT14I CCWWGG 3 cut(s) 259, 594, 2137
EcoT38I GRGCYC 1 cut(s) 1452
ErhI CCWWGG 3 cut(s) 259, 594, 2137
FaeI CATG 6 cut(s) 147, 232, 598, 625, 1064, 1584
FaqI GGGAC 2 cut(s) 1536, 1606
FatI CATG 6 cut(s) 143, 228, 594, 621, 1060, 1580
FauI CCCGC 3 cut(s) 197, 1413, 2078
FblI GTMKAC 3 cut(s) 1017, 1273, 1537
Fnu4HI GCNGC 8 cut(s) 159, 724, 795, 1168, 1239, 2006, 2147, 2150
FokI GGATG 5 cut(s) 497, 529, 919, 1363, 2053
FriOI GRGCYC 1 cut(s) 1452
Fsp4HI GCNGC 8 cut(s) 159, 724, 795, 1168, 1239, 2006, 2147, 2150
GlaI GCGC 3 cut(s) 984, 1504, 2110
GluI GCNGC 8 cut(s) 159, 724, 795, 1168, 1239, 2006, 2147, 2150
GsuI CTGGAG 1 cut(s) 1800
HaeII RGCGCY 1 cut(s) 2112
HaeIII GGCC 4 cut(s) 456, 1435, 1474, 2122
HapII CCGG 2 cut(s) 369, 1515
HgaI GACGC 1 cut(s) 2021
HhaI GCGC 3 cut(s) 985, 1505, 2111
Hin1II CATG 6 cut(s) 147, 232, 598, 625, 1064, 1584
Hin6I GCGC 3 cut(s) 983, 1503, 2109
HinP1I GCGC 3 cut(s) 983, 1503, 2109
HindIII AAGCTT 1 cut(s) 381
HpaII CCGG 2 cut(s) 369, 1515
HphI GGTGA 4 cut(s) 383, 452, 684, 1128
Hpy166II GTNNAC 6 cut(s) 814, 1018, 1258, 1274, 1538, 2134
Hpy8I GTNNAC 6 cut(s) 814, 1018, 1258, 1274, 1538, 2134
Hpy99I CGWCG 5 cut(s) 687, 1131, 1482, 1731, 2121
HpyAV CCTTC 5 cut(s) 1809, 1959, 1970, 2015, 2160
HpyCH4III ACNGT 4 cut(s) 1460, 1682, 1922, 2096
HpyCH4IV ACGT 6 cut(s) 121, 234, 682, 1126, 1477, 1726
HpyCH4V TGCA 9 cut(s) 192, 401, 420, 806, 1060, 1208, 1250, 1580, 2066
HpyF10VI GCNNNNNNNGC 3 cut(s) 453, 803, 1247
HpyF3I CTNAG 4 cut(s) 411, 570, 1909, 2175
HpySE526I ACGT 6 cut(s) 121, 234, 682, 1126, 1477, 1726
Hsp92II CATG 6 cut(s) 147, 232, 598, 625, 1064, 1584
HspAI GCGC 3 cut(s) 983, 1503, 2109
LmnI GCTCC 2 cut(s) 404, 1688
Lsp1109I GCAGC 3 cut(s) 145, 2158, 2161
LweI GCATC 4 cut(s) 145, 994, 1514, 2075
MaeII ACGT 6 cut(s) 121, 234, 682, 1126, 1477, 1726
MaeIII GTNAC 3 cut(s) 146, 2042, 2170
MfeI CAATTG 1 cut(s) 362
MflI RGATCY 7 cut(s) 353, 469, 608, 769, 884, 1213, 1328
MhlI GDGCHC 2 cut(s) 1452, 1668
MluCI AATT 8 cut(s) 104, 197, 362, 847, 1291, 1747, 1789, 1989
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 2 cut(s) 16, 1849
MseI TTAA 2 cut(s) 225, 459
MslI CAYNNNNRTG 4 cut(s) 227, 438, 819, 1263
MspI CCGG 2 cut(s) 369, 1515
MspR9I CCNGG 4 cut(s) 369, 995, 1489, 1515
MunI CAATTG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 194
MvaI CCWGG 2 cut(s) 995, 1489
MwoI GCNNNNNNNGC 3 cut(s) 453, 803, 1247
NciI CCSGG 2 cut(s) 369, 1515
NcoI CCATGG 1 cut(s) 594
NlaIII CATG 6 cut(s) 147, 232, 598, 625, 1064, 1584
NlaIV GGNNCC 6 cut(s) 709, 771, 1153, 1215, 1524, 1622
NmuCI GTSAC 1 cut(s) 146
NspI RCATGY 2 cut(s) 147, 625
OliI CACNNNNGTG 2 cut(s) 819, 1263
PaeI GCATGC 1 cut(s) 625
PaeR7I CTCGAG 1 cut(s) 2124
PceI AGGCCT 1 cut(s) 1435
PciI ACATGT 1 cut(s) 143
PcsI WCGNNNNNNNCGW 2 cut(s) 2005, 2122
PctI GAATGC 1 cut(s) 194
PflMI CCANNNNNTGG 2 cut(s) 740, 1184
PkrI GCNGC 8 cut(s) 160, 725, 796, 1169, 1240, 2007, 2148, 2151
PleI GAGTC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 539
PpuMI RGGWCCY 3 cut(s) 1003, 1523, 1620
PscI ACATGT 1 cut(s) 143
PsiI TTATAA 2 cut(s) 846, 1290
Psp124BI GAGCTC 1 cut(s) 1452
Psp5II RGGWCCY 3 cut(s) 1003, 1523, 1620
Psp6I CCWGG 2 cut(s) 993, 1487
PspGI CCWGG 2 cut(s) 993, 1487
PspN4I GGNNCC 6 cut(s) 709, 771, 1153, 1215, 1524, 1622
PspPI GGNCC 4 cut(s) 1003, 1472, 1523, 1620
PspPPI RGGWCCY 3 cut(s) 1003, 1523, 1620
PstI CTGCAG 1 cut(s) 403
PsuI RGATCY 7 cut(s) 353, 469, 608, 769, 884, 1213, 1328
RsaI GTAC 3 cut(s) 84, 237, 298
RsaNI GTAC 3 cut(s) 83, 236, 297
RseI CAYNNNNRTG 4 cut(s) 227, 438, 819, 1263
SacI GAGCTC 1 cut(s) 1452
SaqAI TTAA 2 cut(s) 225, 459
SatI GCNGC 8 cut(s) 159, 724, 795, 1168, 1239, 2006, 2147, 2150
Sau96I GGNCC 4 cut(s) 1003, 1472, 1523, 1620
SchI GAGTC 1 cut(s) 539
ScrFI CCNGG 4 cut(s) 369, 995, 1489, 1515
SduI GDGCHC 2 cut(s) 1452, 1668
SfaNI GCATC 4 cut(s) 145, 994, 1514, 2075
SfcI CTRYAG 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 2124
SinI GGWCC 3 cut(s) 1003, 1523, 1620
SlaI CTCGAG 1 cut(s) 2124
SmiMI CAYNNNNRTG 4 cut(s) 227, 438, 819, 1263
SmlI CTYRAG 1 cut(s) 2124
SmoI CTYRAG 1 cut(s) 2124
SphI GCATGC 1 cut(s) 625
Sse9I AATT 8 cut(s) 104, 197, 362, 847, 1291, 1747, 1789, 1989
SseBI AGGCCT 1 cut(s) 1435
SsiI CCGC 8 cut(s) 204, 724, 794, 1168, 1238, 1420, 2005, 2071
SstI GAGCTC 1 cut(s) 1452
StuI AGGCCT 1 cut(s) 1435
StyD4I CCNGG 4 cut(s) 367, 993, 1487, 1513
StyI CCWWGG 3 cut(s) 259, 594, 2137
TaaI ACNGT 4 cut(s) 1460, 1682, 1922, 2096
TaiI ACGT 6 cut(s) 124, 237, 685, 1129, 1480, 1729
TaqI TCGA 6 cut(s) 747, 1047, 1191, 1567, 2116, 2125
TasI AATT 8 cut(s) 104, 197, 362, 847, 1291, 1747, 1789, 1989
TauI GCSGC 5 cut(s) 726, 797, 1170, 1241, 2008
Tru1I TTAA 2 cut(s) 225, 459
Tru9I TTAA 2 cut(s) 225, 459
TscAI CASTG 7 cut(s) 157, 199, 307, 333, 1854, 1927, 1957
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 3 cut(s) 158, 2146, 2149
Tsp45I GTSAC 1 cut(s) 146
TspGWI ACGGA 4 cut(s) 38, 1083, 1603, 2030
TspRI CASTG 7 cut(s) 157, 199, 307, 333, 1854, 1927, 1957
Van91I CCANNNNNTGG 2 cut(s) 740, 1184
VpaK11BI GGWCC 3 cut(s) 1003, 1523, 1620
XagI CCTNNNNNAGG 2 cut(s) 511, 998
XapI RAATTY 2 cut(s) 104, 197
XbaI TCTAGA 2 cut(s) 1033, 1553
XceI RCATGY 2 cut(s) 147, 625
XhoI CTCGAG 1 cut(s) 2124
XmaJI CCTAGG 2 cut(s) 259, 2137
XmiI GTMKAC 3 cut(s) 1017, 1273, 1537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.