pycom07g25280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001112
Physical Location & Seq
Forward (+)
25656356 .. 25656634
279 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25280.1

Sequence Viewer

Length: 279 bp
ATGATTCGTCCAGCAAAATTCTCCGAGTTGCCCAGTGGTGGTAGGGAGTATAGATACGAAGGTAGCGACGATGATAGAAGGAGACCTGGTGGAAGATTTGAGATCGTTCGCCGCGTCAGGCGTTTTGATTCAGATGGTGTTGTGCGTCAGTTCCGTTTTGATGAAGACCGTTTTGCATCTCGCAACACACAGAACTACGATGAATCTGACAGCAGGGCAACCGAGAGAAGGCCTCGAGAAGCTTACATAGGAGAAGTAGCGAAGAGGAGAGTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

11.02

Weight (kDa)

10.2

Isoelectric Point (pI)

83.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 114
AciI CCGC 1 cut(s) 112
AcsI RAATTY 1 cut(s) 17
AfiI CCNNNNNNNGG 2 cut(s) 38, 228
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 234
AoxI GGCC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 234
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BbsI GAAGAC 1 cut(s) 171
BccI CCATC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 76
BisI GCNGC 1 cut(s) 112
BlsI GCNGC 1 cut(s) 113
Bme1390I CCNGG 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 87
BmrI ACTGGG 1 cut(s) 27
BmsI GCATC 1 cut(s) 185
BmuI ACTGGG 1 cut(s) 27
BpiI GAAGAC 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 217, 249
BsaI GGTCTC 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 228
Bse1I ACTGG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 87
BseLI CCNNNNNNNGG 2 cut(s) 38, 228
BseNI ACTGG 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 232
BsiHKCI CYCGRG 1 cut(s) 234
BslI CCNNNNNNNGG 2 cut(s) 38, 228
BsmAI GTCTC 1 cut(s) 76
BsnI GGCC 1 cut(s) 232
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 234
Bsp143I GATC 1 cut(s) 102
BspACI CCGC 1 cut(s) 112
BspANI GGCC 1 cut(s) 232
BspFNI CGCG 1 cut(s) 114
BspTNI GGTCTC 1 cut(s) 76
BsrI ACTGG 1 cut(s) 33
BssMI GATC 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 257
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 1 cut(s) 105
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 1 cut(s) 102
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstUI CGCG 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 171
BsuRI GGCC 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 40
CseI GACGC 2 cut(s) 103, 134
CsiI ACCWGGT 1 cut(s) 85
CviJI RGCY 2 cut(s) 232, 242
CviKI_1 RGCY 2 cut(s) 232, 242
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Eco147I AGGCCT 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 234
EcoRII CCWGG 1 cut(s) 85
FaiI YATR 2 cut(s) 51, 248
Fnu4HI GCNGC 1 cut(s) 112
Fsp4HI GCNGC 1 cut(s) 112
GluI GCNGC 1 cut(s) 112
HaeIII GGCC 1 cut(s) 232
HgaI GACGC 2 cut(s) 103, 134
HindIII AAGCTT 1 cut(s) 240
HinfI GANTC 3 cut(s) 4, 128, 203
Hpy188I TCNGA 3 cut(s) 25, 133, 208
Hpy188III TCNNGA 1 cut(s) 236
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 3 cut(s) 53, 72, 222
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 1 cut(s) 176
Kzo9I GATC 1 cut(s) 102
LpnPI CCDG 6 cut(s) 24, 46, 72, 99, 103, 199
LweI GCATC 1 cut(s) 185
MabI ACCWGGT 1 cut(s) 85
MalI GATC 1 cut(s) 104
MboI GATC 1 cut(s) 102
MboII GAAGA 3 cut(s) 105, 176, 274
MluCI AATT 2 cut(s) 17, 274
MnlI CCTC 2 cut(s) 243, 258
MseI TTAA 1 cut(s) 277
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 114
NdeII GATC 1 cut(s) 102
PaeR7I CTCGAG 1 cut(s) 234
PceI AGGCCT 1 cut(s) 232
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 111
PfeI GAWTC 3 cut(s) 4, 128, 203
PkrI GCNGC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
SaqAI TTAA 1 cut(s) 277
SatI GCNGC 1 cut(s) 112
Sau3AI GATC 1 cut(s) 102
ScrFI CCNGG 1 cut(s) 87
SetI ASST 3 cut(s) 64, 88, 244
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 1 cut(s) 185
Sfr274I CTCGAG 1 cut(s) 234
SlaI CTCGAG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
Sse9I AATT 2 cut(s) 17, 274
SseBI AGGCCT 1 cut(s) 232
SsiI CCGC 1 cut(s) 112
StuI AGGCCT 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 170
TaqI TCGA 1 cut(s) 235
TasI AATT 2 cut(s) 17, 274
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 3 cut(s) 4, 128, 203
Tru1I TTAA 1 cut(s) 277
Tru9I TTAA 1 cut(s) 277
TscAI CASTG 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 177, 216
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 40
XapI RAATTY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.