MD02G1258400.v1.1

DnaJ protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000572
Physical Location & Seq
Reverse (-)
31163067 .. 31166674
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1258400.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGTTTGGGAGAGCTCCAAAGAAAAGCGATAACAGCAAGTATTATGAGATCCTTGGAGTTTCAAAGACCGCTTCGCAGGACGATCTAAAGAAGGCTTATAGAAAAGCCGCCATCAAGAACCACCCTGATAAGGGCGGCGATCCTGAGAAGTTTAAAGAGTTGGCCCAAGCATATGAGGTTCTGAGTGATCCAGAGAAACGTGAGATATACGATCAATATGGCGAGGATGCCCTCAAGGAAGGAATGGGTGGTGGAGGTGGTGGCCATGACCCATTTGATATTTTCCAATCCTTCTTTGGTGGAAACCCATTCGGTGGTGGTGGAAGCAGCAGAGGCCGAAGGCAGAGAAGGGGAGAGGATGTGATCCATCCCCTTAAGGTTTCTTTGGAAGATCTCTACAATGGAACGTCCAAAAAGCTTTCTCTTGCGCGCAACAAAATCTGCTCCAAGTGCAAGGGTAAAGGGTCGAAGTCAGGTGCTTCAATGAAATGTCCTGGCTGCCAAGGTTCTGGAATGAAAGTCTCTATTAGACATCTCGGCCCCTCGATGATCCAGCAAATGCAACATCCTTGCGCTGAGTGCAAGGGTACTGGTGAGACCATTAATGACAAGGATCGCTGCACACAATGTAAGGGTGAGAAGGTTGTTCAGGAAAAGAAAGTCTTAGAAGTAATTGTGGAGAAGGGCATGCAAAATGGGCAGAAGATTACTTTCCCTGGTGAAGCTGATGAAGCGCCAGACACCATCACAGGCGATATTGTCTTTGTCCTACAACAAAAGGAGCACCCTAAATTTAAGAGGAAGGGTGATGATCTCTTCTTTGAACATACCTTGTCACTTGTGGAATCACTCTGCGGCTTCCAATTTGTATTGACACATTTAGATGGAAGACAGCTCCTCATTAAATCCCAACCTGGAGAAGTTGTAAAGCCTGATCAATTCAAGGCTATAAATGATGAGGGCATGCCAATGTACCAGAGGCCATTCATGAAAGGTAAGTTATATATTCACTTCACAGTGGAGTTCCCCGACTCACTGAATCCAGAACAGTGCAAGGCTCTGGAGGCTGTCCTCCCCCCCAAGACTTCGGCTCAGCTGACCGACATGGAGTTGGATGAATGTGAGGAGACTACGCTGCATGATGTTAACATAGAGGAGGAAATGCGCCGAAAGCAAGCACATGCACACGAGGCATATGACGATGATGAGGACATGCATGGTGGTGCCCAGAGGGTTCAGTGTGCTCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

418

Amino Acids

46.41

Weight (kDa)

6.26

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 13 - 71 9.5e-24 DnaJ domain
DnaJ_C PF01556 123 - 343 8e-36 DnaJ C terminal domain
DnaJ_CXXCXGXG PF00684 148 - 214 2.6e-15 DnaJ central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000572)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44110 AT3G44110 AT5G22060
fragaria_vesca FvH4_3g26170 FvH4_3g26170 FvH4_3g26170 FvH4_3g26220 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270
malus_domestica MD02G1258400.v1.1 MD03G1159100.v1.1 MD07G1062300.v1.1 MD11G1174700.v1.1
prunus_persica Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.6G146900_v2.0.a1 Prupe.6G146900_v2.0.a1
pyrus_communis pycom02g21960 pycom03g11140 pycom07g04780 pycom11g14940
rosa_chinensis RchiOBHm_Chr1g0332041 RchiOBHm_Chr1g0364381 RchiOBHm_Chr4g0428981 RchiOBHm_Chr5g0049001 RchiOBHm_Chr5g0049021
rosa_laevigata RLG00000007108 RLG00000013380 RLG00000027486 RLG00000029697 RLG00000034581 RLG00000034584
rosa_multiflora Rmu_co8449175.1_g000001 Rmu_sc0000441.1_g000032 Rmu_sc0000530.1_g000003 Rmu_sc0003358.1_g000010 Rmu_sc0004058.1_g000007 Rmu_sc0004522.1_g000007 Rmu_sc0008629.1_g000009 Rmu_sc0009489.1_g000007 Rmu_sc0014815.1_g000013
rosa_roxburghii Rroxscaffold_1G00032850 Rroxscaffold_1G00032870 Rroxscaffold_4G00292340 Rroxscaffold_4G00318430 Rroxscaffold_5G00370640
rosa_rugosa Rorug01G0097800 Rorug01G0097900 Rorug01G0098000 Rorug01G0315800 Rorug04G0231300 Rorug05G0241800 Rorug05G0241900 Rorug05G0242000 Rorug05G0242100.1 Rorug05G0242200 Rorug05G0242300 Rorug06G0102700 Rorug06G0102800
rosa_samantha Rh1AG121800 Rh1BG093200 Rh1CG303100 Rh1DG127500 Rh4BG293700 Rh4DG290600 Rh5AG322000 Rh5CG357500 Rh5CG357600 Rh5DG344100 Rh6BG218000
rosa_wichuraiana Rw0G008830 Rw1G009930 Rw1G028700 Rw5G030390 Rw5G030400 Rw6G018630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1223
AccB7I CCANNNNNTGG 1 cut(s) 314
AccII CGCG 1 cut(s) 430
AciI CCGC 4 cut(s) 69, 108, 135, 855
AclWI GGATC 6 cut(s) 43, 134, 182, 358, 544, 621
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 791
AfaI GTAC 2 cut(s) 589, 974
AfiI CCNNNNNNNGG 3 cut(s) 130, 131, 314
AflII CTTAAG 1 cut(s) 374
AgsI TTSAA 4 cut(s) 63, 483, 824, 943
AjnI CCWGG 3 cut(s) 493, 715, 913
AluBI AGCT 5 cut(s) 14, 418, 725, 895, 1096
AluI AGCT 5 cut(s) 14, 418, 725, 895, 1096
Alw21I GWGCWC 3 cut(s) 16, 786, 1246
Alw26I GTCTC 3 cut(s) 526, 590, 1121
AlwI GGATC 6 cut(s) 43, 134, 182, 358, 544, 621
AoxI GGCC 5 cut(s) 162, 262, 334, 538, 980
ApeKI GCWGC 4 cut(s) 327, 498, 618, 1135
ApoI RAATTY 1 cut(s) 791
AseI ATTAAT 1 cut(s) 603
AspLEI GCGC 5 cut(s) 430, 432, 575, 736, 1167
AspS9I GGNCC 2 cut(s) 163, 539
AsuHPI GGTGA 4 cut(s) 605, 647, 731, 818
BaeGI GKGCMC 1 cut(s) 1228
BalI TGGCCA 1 cut(s) 264
BanI GGYRCC 1 cut(s) 1223
BanII GRGCYC 1 cut(s) 16
BauI CACGAG 1 cut(s) 1187
BbsI GAAGAC 1 cut(s) 895
Bbv12I GWGCWC 3 cut(s) 16, 786, 1246
BbvI GCAGC 4 cut(s) 339, 485, 605, 1122
BccI CCATC 4 cut(s) 119, 375, 752, 878
BciT130I CCWGG 3 cut(s) 495, 717, 915
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 3 cut(s) 526, 590, 1121
BfoI RGCGCY 1 cut(s) 737
BfrI CTTAAG 1 cut(s) 374
BglII AGATCT 1 cut(s) 391
BisI GCNGC 7 cut(s) 108, 136, 328, 499, 619, 856, 1136
BlpI GCTNAGC 2 cut(s) 1092, 1245
BlsI GCNGC 7 cut(s) 109, 137, 329, 500, 620, 857, 1137
Bme1390I CCNGG 3 cut(s) 495, 717, 915
BmgT120I GGNCC 2 cut(s) 163, 539
BmiI GGNNCC 2 cut(s) 541, 1225
BmrFI CCNGG 3 cut(s) 495, 717, 915
BmsI GCATC 1 cut(s) 217
BpiI GAAGAC 1 cut(s) 895
BpmI CTGGAG 2 cut(s) 936, 1082
Bpu1102I GCTNAGC 2 cut(s) 1092, 1245
BpuEI CTTGAG 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 590
BsaJI CCNNGG 3 cut(s) 52, 502, 715
BsaXI ACNNNNNCTCC 4 cut(s) 345, 375, 909, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 131, 314
Bse1I ACTGG 1 cut(s) 595
BseBI CCWGG 3 cut(s) 495, 717, 915
BseDI CCNNGG 3 cut(s) 52, 502, 715
BseGI GGATG 5 cut(s) 232, 364, 367, 565, 1120
BseLI CCNNNNNNNGG 3 cut(s) 130, 131, 314
BseMII CTCAG 4 cut(s) 135, 173, 567, 1106
BseNI ACTGG 1 cut(s) 595
BsePI GCGCGC 1 cut(s) 428
BseRI GAGGAG 3 cut(s) 887, 1139, 1169
BseSI GKGCMC 1 cut(s) 1228
BseXI GCAGC 4 cut(s) 339, 485, 605, 1122
BsgI GTGCAG 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 430
BshFI GGCC 5 cut(s) 164, 264, 336, 540, 982
BshNI GGYRCC 1 cut(s) 1223
BsiHKAI GWGCWC 3 cut(s) 16, 786, 1246
BslI CCNNNNNNNGG 3 cut(s) 130, 131, 314
BsmAI GTCTC 3 cut(s) 526, 590, 1121
BsnI GGCC 5 cut(s) 164, 264, 336, 540, 982
Bso31I GGTCTC 1 cut(s) 590
Bsp1286I GDGCHC 4 cut(s) 16, 786, 1228, 1246
Bsp1720I GCTNAGC 2 cut(s) 1092, 1245
BspACI CCGC 4 cut(s) 69, 108, 135, 855
BspANI GGCC 5 cut(s) 164, 264, 336, 540, 982
BspCNI CTCAG 4 cut(s) 136, 174, 568, 1105
BspFNI CGCG 1 cut(s) 430
BspHI TCATGA 1 cut(s) 987
BspLI GGNNCC 2 cut(s) 541, 1225
BspPI GGATC 6 cut(s) 43, 134, 182, 358, 544, 621
BspT107I GGYRCC 1 cut(s) 1223
BspTI CTTAAG 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 590
BsrI ACTGG 1 cut(s) 595
BssECI CCNNGG 3 cut(s) 52, 502, 715
BssHII GCGCGC 1 cut(s) 428
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 2 cut(s) 52, 502
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 3 cut(s) 495, 717, 915
Bst4CI ACNGT 2 cut(s) 1018, 1050
Bst6I CTCTTC 1 cut(s) 821
BstAFI CTTAAG 1 cut(s) 374
BstC8I GCNNGC 4 cut(s) 430, 689, 965, 1176
BstDEI CTNAG 6 cut(s) 144, 182, 576, 664, 1092, 1245
BstF5I GGATG 5 cut(s) 232, 364, 367, 565, 1120
BstFNI CGCG 1 cut(s) 430
BstH2I RGCGCY 1 cut(s) 737
BstHHI GCGC 5 cut(s) 430, 432, 575, 736, 1167
BstMAI GTCTC 3 cut(s) 526, 590, 1121
BstMWI GCNNNNNNNGC 9 cut(s) 33, 333, 450, 579, 697, 731, 1064, 1171, 1190
BstNI CCWGG 3 cut(s) 495, 717, 915
BstNSI RCATGY 4 cut(s) 691, 967, 1184, 1216
BstSCI CCNGG 3 cut(s) 493, 715, 913
BstSLI GKGCMC 1 cut(s) 1228
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 4 cut(s) 339, 485, 605, 1122
BstV2I GAAGAC 1 cut(s) 895
BstX2I RGATCY 2 cut(s) 48, 391
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 2 cut(s) 48, 391
BsuRI GGCC 5 cut(s) 164, 264, 336, 540, 982
BtsCI GGATG 5 cut(s) 232, 364, 367, 565, 1120
BtsIMutI CAGTG 4 cut(s) 1023, 1034, 1055, 1244
Cac8I GCNNGC 4 cut(s) 430, 689, 965, 1176
CciI TCATGA 1 cut(s) 987
CfoI GCGC 5 cut(s) 430, 432, 575, 736, 1167
Cfr13I GGNCC 2 cut(s) 163, 539
Csp6I GTAC 2 cut(s) 588, 973
CviAII CATG 9 cut(s) 266, 688, 964, 988, 1105, 1139, 1181, 1213, 1217
CviQI GTAC 2 cut(s) 588, 973
DdeI CTNAG 6 cut(s) 144, 182, 576, 664, 1092, 1245
DraI TTTAAA 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 821
EarI CTCTTC 1 cut(s) 821
Ecl136II GAGCTC 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 52, 502
Eco24I GRGCYC 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 590
Eco53kI GAGCTC 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 14
EcoRII CCWGG 3 cut(s) 493, 715, 913
EcoT14I CCWWGG 2 cut(s) 52, 502
EcoT22I ATGCAT 1 cut(s) 1218
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 52, 502
FaeI CATG 9 cut(s) 269, 691, 967, 991, 1108, 1142, 1184, 1216, 1220
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FatI CATG 9 cut(s) 265, 687, 963, 987, 1104, 1138, 1180, 1212, 1216
FauNDI CATATG 2 cut(s) 172, 1195
FbaI TGATCA 1 cut(s) 934
Fnu4HI GCNGC 7 cut(s) 108, 136, 328, 499, 619, 856, 1136
FokI GGATG 5 cut(s) 239, 354, 371, 552, 1127
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 7 cut(s) 108, 136, 328, 499, 619, 856, 1136
GlaI GCGC 5 cut(s) 429, 431, 574, 735, 1166
GluI GCNGC 7 cut(s) 108, 136, 328, 499, 619, 856, 1136
GsuI CTGGAG 2 cut(s) 936, 1082
HaeII RGCGCY 1 cut(s) 737
HaeIII GGCC 5 cut(s) 164, 264, 336, 540, 982
HhaI GCGC 5 cut(s) 430, 432, 575, 736, 1167
Hin1II CATG 9 cut(s) 269, 691, 967, 991, 1108, 1142, 1184, 1216, 1220
Hin6I GCGC 5 cut(s) 428, 430, 573, 734, 1165
HinP1I GCGC 5 cut(s) 428, 430, 573, 734, 1165
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 3 cut(s) 845, 1031, 1039
HpaI GTTAAC 1 cut(s) 1147
HphI GGTGA 4 cut(s) 605, 647, 731, 818
Hpy166II GTNNAC 1 cut(s) 1147
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 8 cut(s) 115, 143, 191, 510, 650, 988, 1043, 1061
Hpy8I GTNNAC 1 cut(s) 1147
HpyAV CCTTC 8 cut(s) 85, 233, 301, 333, 342, 634, 676, 796
HpyCH4III ACNGT 2 cut(s) 1018, 1050
HpyCH4IV ACGT 2 cut(s) 199, 407
HpyCH4V TGCA 9 cut(s) 453, 562, 582, 621, 691, 1053, 1138, 1184, 1216
HpyF10VI GCNNNNNNNGC 9 cut(s) 33, 333, 450, 579, 697, 731, 1064, 1171, 1190
HpyF3I CTNAG 6 cut(s) 144, 182, 576, 664, 1092, 1245
HpySE526I ACGT 2 cut(s) 199, 407
Hsp92II CATG 9 cut(s) 269, 691, 967, 991, 1108, 1142, 1184, 1216, 1220
HspAI GCGC 5 cut(s) 428, 430, 573, 734, 1165
Ksp22I TGATCA 1 cut(s) 934
KspAI GTTAAC 1 cut(s) 1147
LmnI GCTCC 4 cut(s) 19, 449, 781, 900
Lsp1109I GCAGC 4 cut(s) 339, 485, 605, 1122
LweI GCATC 1 cut(s) 217
MaeII ACGT 2 cut(s) 199, 407
MaeIII GTNAC 1 cut(s) 834
MboII GAAGA 4 cut(s) 401, 715, 808, 900
MflI RGATCY 2 cut(s) 48, 391
MhlI GDGCHC 4 cut(s) 16, 786, 1228, 1246
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 4 cut(s) 672, 791, 863, 938
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 1 cut(s) 1092
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 264
MseI TTAA 6 cut(s) 153, 375, 603, 795, 903, 1146
MslI CAYNNNNRTG 3 cut(s) 882, 968, 1221
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 1096
MspCI CTTAAG 1 cut(s) 374
MspR9I CCNGG 3 cut(s) 495, 717, 915
MvaI CCWGG 3 cut(s) 495, 717, 915
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 9 cut(s) 33, 333, 450, 579, 697, 731, 1064, 1171, 1190
NdeI CATATG 2 cut(s) 172, 1195
NlaIII CATG 9 cut(s) 269, 691, 967, 991, 1108, 1142, 1184, 1216, 1220
NlaIV GGNNCC 2 cut(s) 541, 1225
NmeAIII GCCGAG 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 834
NsiI ATGCAT 1 cut(s) 1218
NspI RCATGY 4 cut(s) 691, 967, 1184, 1216
PaeI GCATGC 2 cut(s) 691, 967
PagI TCATGA 1 cut(s) 987
PauI GCGCGC 1 cut(s) 428
PfeI GAWTC 2 cut(s) 845, 1039
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 7 cut(s) 109, 137, 329, 500, 620, 857, 1137
PleI GAGTC 1 cut(s) 1025
PpsI GAGTC 1 cut(s) 1025
PshBI ATTAAT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 16
Psp6I CCWGG 3 cut(s) 493, 715, 913
PspGI CCWGG 3 cut(s) 493, 715, 913
PspN4I GGNNCC 2 cut(s) 541, 1225
PspPI GGNCC 2 cut(s) 163, 539
PsuI RGATCY 2 cut(s) 48, 391
PteI GCGCGC 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 1096
RsaI GTAC 2 cut(s) 589, 974
RsaNI GTAC 2 cut(s) 588, 973
RseI CAYNNNNRTG 3 cut(s) 882, 968, 1221
SacI GAGCTC 1 cut(s) 16
SaqAI TTAA 6 cut(s) 153, 375, 603, 795, 903, 1146
SatI GCNGC 7 cut(s) 108, 136, 328, 499, 619, 856, 1136
Sau96I GGNCC 2 cut(s) 163, 539
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 3 cut(s) 495, 717, 915
SduI GDGCHC 4 cut(s) 16, 786, 1228, 1246
SfaNI GCATC 1 cut(s) 217
SmiMI CAYNNNNRTG 3 cut(s) 882, 968, 1221
SmlI CTYRAG 2 cut(s) 233, 374
SmoI CTYRAG 2 cut(s) 233, 374
SphI GCATGC 2 cut(s) 691, 967
Sse9I AATT 4 cut(s) 672, 791, 863, 938
SsiI CCGC 4 cut(s) 69, 108, 135, 855
SstI GAGCTC 1 cut(s) 16
StyD4I CCNGG 3 cut(s) 493, 715, 913
StyI CCWWGG 2 cut(s) 52, 502
TaaI ACNGT 2 cut(s) 1018, 1050
TaiI ACGT 2 cut(s) 202, 410
TaqI TCGA 2 cut(s) 467, 545
TaqII GACCGA 1 cut(s) 1115
TasI AATT 4 cut(s) 672, 791, 863, 938
TauI GCSGC 3 cut(s) 110, 138, 858
TfiI GAWTC 2 cut(s) 845, 1039
Tru1I TTAA 6 cut(s) 153, 375, 603, 795, 903, 1146
Tru9I TTAA 6 cut(s) 153, 375, 603, 795, 903, 1146
TscAI CASTG 4 cut(s) 1023, 1041, 1055, 1244
TseFI GTSAC 1 cut(s) 834
TseI GCWGC 4 cut(s) 327, 498, 618, 1135
Tsp45I GTSAC 1 cut(s) 834
TspDTI ATGAA 6 cut(s) 500, 530, 744, 976, 1004, 1131
TspRI CASTG 4 cut(s) 1023, 1041, 1055, 1244
Van91I CCANNNNNTGG 1 cut(s) 314
Vha464I CTTAAG 1 cut(s) 374
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 1 cut(s) 791
XceI RCATGY 4 cut(s) 691, 967, 1184, 1216
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
Zsp2I ATGCAT 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.