Rh1BG093200

DnaJ protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000572
Physical Location & Seq
Forward (+)
16319447 .. 16322099
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG093200.1

Sequence Viewer

Length: 1254 bp
ATGTTTGGGAGAGCTCCAAAGAAAAGCGACAACACCAAGTACTATGAGATCCTTGGAGTGCCTAAGAACGCGTCGCAGGATGATCTGAAGAAGGCTTACAGAAAAGCAGCCATCAAGAACCATCCTGATAAGGGTGGGGATCCAGAGAAGTTTAAAGAGTTGGCCCAAGCATACGAGGTTCTGAGTGATCCAGAGAAACGGGAAGTGTATGACCAATATGGCGAGGATGCACTCAAGGAAGGAATGGGTGCCGGAGGCGGCGGCCACGACCCATTTGATATATTCCAGTCCTTCTTTGGTGGCAACCCATTTGGCGGGGGTGGCAGCAGCAGAGGCCGTAGGCAGAGAAGGGGAGAGGATGTGATCCATCCCCTTAAAGTTTCTCTGGAAGATCTATATAATGGGACATCCAAGAAGCTGTCTCTCTCTCGTAACAAAATCTGTGCCAAGTGCAAGGGTAAAGGGTCAAAGTCGGGTGCTTCAATGAAGTGTCCAGGTTGCCAAGGGTCTGGAATGAAAGTCTCTATTAGACATCTTGGACCTTCCATGATCCAGCAAATGCAGCATCCATGCAATGAGTGCAAGGGTACAGGTGAAACCATTAATGACAAGGATCGCTGTCCGCAATGTAAGGGTGAGAAAGTTGTTCAGGAAAAGAAAGTATTGGAAGTCCATGTGGAGAAGGGAATGCAAAATGGACAGAGAATCACATTCCCTGGTGAAGCTGATGAAGCGCCTGACACCATCACAGGAGATATTGTTTTTGTCCTGCAACAAAAGGAACACCCTAAGTTTAAGCGAAAGGGTGATGACCTATTCTACGAACATACGCTGTCCCTTATGGAGGCGCTCTGCGGCTTCCAATTTATCTTCACGCATTTAGATGGCAGGCAACTCCTCATCAAATCTCATCCTGGAGAAGTTGTCAAGCCTGATCAATTCAAGGCTATAAATGATGAAGGCATGCCGATGTACCAGAGGCCATTTATGAAGGGTAAACTATATATACATTTCACTGTGGAGTTCCCAGATTCATTGAAGCCAGAACAGTGCAAGGCCTTGGAAGCAGTGCTGCCGAGGAGTTCTGCCCAGCTTACTGACATGGAACTAGATGAGTGTGAGGAGACTACACTGCATGATGTCAACATAGAAGAGGAAATGCGTCGCAAACAAGCACAGCAGTCTCAAGAGGCATACGACGAGGATGAGGATATGCATGGTGGTGCCCAGAGGGTGCAATGCGCTCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

417

Amino Acids

46.7

Weight (kDa)

6.38

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 13 - 71 1.4e-23 DnaJ domain
DnaJ_C PF01556 123 - 343 1.1e-37 DnaJ C terminal domain
DnaJ_CXXCXGXG PF00684 148 - 214 1.3e-15 DnaJ central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000572)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44110 AT3G44110 AT5G22060
fragaria_vesca FvH4_3g26170 FvH4_3g26170 FvH4_3g26170 FvH4_3g26220 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270
malus_domestica MD02G1258400.v1.1 MD03G1159100.v1.1 MD07G1062300.v1.1 MD11G1174700.v1.1
prunus_persica Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.6G146900_v2.0.a1 Prupe.6G146900_v2.0.a1
pyrus_communis pycom02g21960 pycom03g11140 pycom07g04780 pycom11g14940
rosa_chinensis RchiOBHm_Chr1g0332041 RchiOBHm_Chr1g0364381 RchiOBHm_Chr4g0428981 RchiOBHm_Chr5g0049001 RchiOBHm_Chr5g0049021
rosa_laevigata RLG00000007108 RLG00000013380 RLG00000027486 RLG00000029697 RLG00000034581 RLG00000034584
rosa_multiflora Rmu_co8449175.1_g000001 Rmu_sc0000441.1_g000032 Rmu_sc0000530.1_g000003 Rmu_sc0003358.1_g000010 Rmu_sc0004058.1_g000007 Rmu_sc0004522.1_g000007 Rmu_sc0008629.1_g000009 Rmu_sc0009489.1_g000007 Rmu_sc0014815.1_g000013
rosa_roxburghii Rroxscaffold_1G00032850 Rroxscaffold_1G00032870 Rroxscaffold_4G00292340 Rroxscaffold_4G00318430 Rroxscaffold_5G00370640
rosa_rugosa Rorug01G0097800 Rorug01G0097900 Rorug01G0098000 Rorug01G0315800 Rorug04G0231300 Rorug05G0241800 Rorug05G0241900 Rorug05G0242000 Rorug05G0242100.1 Rorug05G0242200 Rorug05G0242300 Rorug06G0102700 Rorug06G0102800
rosa_samantha Rh1AG121800 Rh1BG093200 Rh1CG303100 Rh1DG127500 Rh4BG293700 Rh4DG290600 Rh5AG322000 Rh5CG357500 Rh5CG357600 Rh5DG344100 Rh6BG218000
rosa_wichuraiana Rw0G008830 Rw1G009930 Rw1G028700 Rw5G030390 Rw5G030400 Rw6G018630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 248, 1223
AccII CGCG 1 cut(s) 71
AciI CCGC 5 cut(s) 258, 261, 315, 623, 855
AclWI GGATC 7 cut(s) 43, 134, 147, 182, 358, 544, 621
AcoI YGGCCR 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 3 cut(s) 41, 589, 974
AfiI CCNNNNNNNGG 3 cut(s) 131, 314, 844
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 3 cut(s) 483, 943, 1039
AjnI CCWGG 3 cut(s) 493, 715, 913
AluBI AGCT 4 cut(s) 14, 418, 725, 1093
AluI AGCT 4 cut(s) 14, 418, 725, 1093
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 4 cut(s) 426, 526, 1118, 1188
AlwI GGATC 7 cut(s) 43, 134, 147, 182, 358, 544, 621
AoxI GGCC 5 cut(s) 162, 262, 334, 980, 1056
ApeKI GCWGC 5 cut(s) 107, 324, 327, 562, 1072
AseI ATTAAT 1 cut(s) 603
AspLEI GCGC 3 cut(s) 736, 850, 1244
AspS9I GGNCC 2 cut(s) 163, 539
AsuHPI GGTGA 4 cut(s) 605, 647, 731, 818
AvaII GGWCC 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 1228
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 2 cut(s) 248, 1223
BanII GRGCYC 1 cut(s) 16
BarI GAAGNNNNNNTAC 2 cut(s) 80, 112
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 5 cut(s) 119, 336, 339, 574, 1059
BccI CCATC 5 cut(s) 119, 129, 375, 752, 878
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 3 cut(s) 495, 717, 915
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 4 cut(s) 426, 526, 1118, 1188
BfaI CTAG 1 cut(s) 1109
BfoI RGCGCY 2 cut(s) 737, 851
BglII AGATCT 1 cut(s) 391
BisI GCNGC 8 cut(s) 108, 259, 262, 325, 328, 563, 856, 1073
BlsI GCNGC 8 cut(s) 109, 260, 263, 326, 329, 564, 857, 1074
BmcAI AGTACT 1 cut(s) 41
Bme1390I CCNGG 3 cut(s) 495, 717, 915
Bme18I GGWCC 1 cut(s) 539
BmgT120I GGNCC 2 cut(s) 163, 539
BmiI GGNNCC 3 cut(s) 141, 250, 1225
BmrFI CCNGG 3 cut(s) 495, 717, 915
BmsI GCATC 2 cut(s) 217, 574
BpmI CTGGAG 1 cut(s) 936
BpuEI CTTGAG 2 cut(s) 218, 1170
BsaJI CCNNGG 5 cut(s) 52, 502, 715, 1059, 1076
BsaXI ACNNNNNCTCC 6 cut(s) 345, 375, 744, 774, 909, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 314, 844
Bse1I ACTGG 1 cut(s) 286
Bse3DI GCAATG 3 cut(s) 580, 632, 1244
BseBI CCWGG 3 cut(s) 495, 717, 915
BseDI CCNNGG 5 cut(s) 52, 502, 715, 1059, 1076
BseGI GGATG 9 cut(s) 85, 121, 232, 364, 367, 407, 565, 910, 1210
BseLI CCNNNNNNNGG 3 cut(s) 131, 314, 844
BseMI GCAATG 3 cut(s) 580, 632, 1244
BseMII CTCAG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 3 cut(s) 887, 1093, 1136
BseSI GKGCMC 1 cut(s) 1228
BseXI GCAGC 5 cut(s) 119, 336, 339, 574, 1059
BseYI CCCAGC 1 cut(s) 1089
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 5 cut(s) 164, 264, 336, 982, 1058
BshNI GGYRCC 2 cut(s) 248, 1223
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 1 cut(s) 252
BslFI GGGAC 2 cut(s) 418, 820
BslI CCNNNNNNNGG 3 cut(s) 131, 314, 844
BsmAI GTCTC 4 cut(s) 426, 526, 1118, 1188
BsmFI GGGAC 2 cut(s) 418, 820
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 5 cut(s) 164, 264, 336, 982, 1058
Bsp1286I GDGCHC 2 cut(s) 16, 1228
Bsp143I GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
BspACI CCGC 5 cut(s) 258, 261, 315, 623, 855
BspANI GGCC 5 cut(s) 164, 264, 336, 982, 1058
BspCNI CTCAG 1 cut(s) 174
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 3 cut(s) 141, 250, 1225
BspPI GGATC 7 cut(s) 43, 134, 147, 182, 358, 544, 621
BspT107I GGYRCC 2 cut(s) 248, 1223
BsrDI GCAATG 3 cut(s) 580, 632, 1244
BsrI ACTGG 1 cut(s) 286
BssECI CCNNGG 5 cut(s) 52, 502, 715, 1059, 1076
BssMI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
BssT1I CCWWGG 3 cut(s) 52, 502, 1059
Bst2UI CCWGG 3 cut(s) 495, 717, 915
Bst4CI ACNGT 2 cut(s) 1018, 1050
Bst6I CTCTTC 1 cut(s) 1146
BstAPI GCANNNNNTGC 1 cut(s) 579
BstC8I GCNNGC 2 cut(s) 890, 965
BstDEI CTNAG 3 cut(s) 63, 182, 789
BstENI CCTNNNNNAGG 1 cut(s) 842
BstF5I GGATG 9 cut(s) 85, 121, 232, 364, 367, 407, 565, 910, 1210
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 2 cut(s) 737, 851
BstHHI GCGC 3 cut(s) 736, 850, 1244
BstKTI GATC 9 cut(s) 51, 85, 142, 190, 366, 394, 552, 616, 937
BstMAI GTCTC 4 cut(s) 426, 526, 1118, 1188
BstMBI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
BstMWI GCNNNNNNNGC 6 cut(s) 321, 333, 562, 579, 731, 1064
BstNI CCWGG 3 cut(s) 495, 717, 915
BstNSI RCATGY 1 cut(s) 967
BstSCI CCNGG 3 cut(s) 493, 715, 913
BstSLI GKGCMC 1 cut(s) 1228
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 5 cut(s) 119, 336, 339, 574, 1059
BstX2I RGATCY 3 cut(s) 48, 139, 391
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 3 cut(s) 48, 139, 391
BsuRI GGCC 5 cut(s) 164, 264, 336, 982, 1058
BtsCI GGATG 9 cut(s) 85, 121, 232, 364, 367, 407, 565, 910, 1210
BtsI GCAGTG 2 cut(s) 1074, 1130
BtsIMutI CAGTG 4 cut(s) 1014, 1055, 1074, 1130
Cac8I GCNNGC 2 cut(s) 890, 965
CfoI GCGC 3 cut(s) 736, 850, 1244
Cfr13I GGNCC 2 cut(s) 163, 539
CseI GACGC 2 cut(s) 60, 1151
Csp6I GTAC 3 cut(s) 40, 588, 973
CviAII CATG 7 cut(s) 547, 570, 674, 964, 1102, 1136, 1217
CviQI GTAC 3 cut(s) 40, 588, 973
DdeI CTNAG 3 cut(s) 63, 182, 789
DpnI GATC 9 cut(s) 50, 84, 141, 189, 365, 393, 551, 615, 936
DpnII GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
DraI TTTAAA 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 1146
EarI CTCTTC 1 cut(s) 1146
Ecl136II GAGCTC 1 cut(s) 14
Eco130I CCWWGG 3 cut(s) 52, 502, 1059
Eco147I AGGCCT 1 cut(s) 1058
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 14
EcoNI CCTNNNNNAGG 1 cut(s) 842
EcoRII CCWGG 3 cut(s) 493, 715, 913
EcoT14I CCWWGG 3 cut(s) 52, 502, 1059
EcoT22I ATGCAT 1 cut(s) 1218
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 3 cut(s) 52, 502, 1059
FaeI CATG 7 cut(s) 550, 573, 677, 967, 1105, 1139, 1220
FaqI GGGAC 2 cut(s) 418, 820
FatI CATG 7 cut(s) 546, 569, 673, 963, 1101, 1135, 1216
FauI CCCGC 1 cut(s) 308
FbaI TGATCA 1 cut(s) 934
Fnu4HI GCNGC 8 cut(s) 108, 259, 262, 325, 328, 563, 856, 1073
FokI GGATG 9 cut(s) 92, 108, 239, 354, 371, 394, 552, 897, 1217
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 8 cut(s) 108, 259, 262, 325, 328, 563, 856, 1073
FspBI CTAG 1 cut(s) 1109
GlaI GCGC 3 cut(s) 735, 849, 1243
GluI GCNGC 8 cut(s) 108, 259, 262, 325, 328, 563, 856, 1073
GsaI CCCAGC 1 cut(s) 1093
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 2 cut(s) 737, 851
HaeIII GGCC 5 cut(s) 164, 264, 336, 982, 1058
HapII CCGG 1 cut(s) 252
HgaI GACGC 2 cut(s) 60, 1151
HhaI GCGC 3 cut(s) 736, 850, 1244
Hin1II CATG 7 cut(s) 550, 573, 677, 967, 1105, 1139, 1220
Hin6I GCGC 3 cut(s) 734, 848, 1242
HinP1I GCGC 3 cut(s) 734, 848, 1242
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HinfI GANTC 2 cut(s) 705, 1031
HpaII CCGG 1 cut(s) 252
HphI GGTGA 4 cut(s) 605, 647, 731, 818
Hpy166II GTNNAC 2 cut(s) 998, 1144
Hpy188I TCNGA 2 cut(s) 87, 183
Hpy188III TCNNGA 8 cut(s) 115, 125, 143, 191, 386, 510, 650, 1187
Hpy8I GTNNAC 2 cut(s) 998, 1144
Hpy99I CGWCG 3 cut(s) 76, 1167, 1202
HpyAV CCTTC 8 cut(s) 85, 233, 301, 342, 552, 676, 953, 985
HpyCH4III ACNGT 2 cut(s) 1018, 1050
HpyF10VI GCNNNNNNNGC 6 cut(s) 321, 333, 562, 579, 731, 1064
HpyF3I CTNAG 3 cut(s) 63, 182, 789
Hsp92II CATG 7 cut(s) 550, 573, 677, 967, 1105, 1139, 1220
HspAI GCGC 3 cut(s) 734, 848, 1242
Ksp22I TGATCA 1 cut(s) 934
Kzo9I GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 5 cut(s) 119, 336, 339, 574, 1059
LweI GCATC 2 cut(s) 217, 574
MaeI CTAG 1 cut(s) 1109
MaeIII GTNAC 1 cut(s) 431
MalI GATC 9 cut(s) 50, 84, 141, 189, 365, 393, 551, 615, 936
MboI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
MboII GAAGA 4 cut(s) 100, 401, 862, 1163
MflI RGATCY 3 cut(s) 48, 139, 391
MhlI GDGCHC 2 cut(s) 16, 1228
MluCI AATT 2 cut(s) 863, 938
MluI ACGCGT 1 cut(s) 69
Mph1103I ATGCAT 1 cut(s) 1218
MseI TTAA 4 cut(s) 153, 375, 603, 795
MslI CAYNNNNRTG 3 cut(s) 882, 968, 1221
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 3 cut(s) 495, 717, 915
Mva1269I GAATGC 1 cut(s) 693
MvaI CCWGG 3 cut(s) 495, 717, 915
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 6 cut(s) 321, 333, 562, 579, 731, 1064
NdeII GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
NlaIII CATG 7 cut(s) 550, 573, 677, 967, 1105, 1139, 1220
NlaIV GGNNCC 3 cut(s) 141, 250, 1225
NmeAIII GCCGAG 1 cut(s) 1101
NsiI ATGCAT 1 cut(s) 1218
NspI RCATGY 1 cut(s) 967
PaeI GCATGC 1 cut(s) 967
PceI AGGCCT 1 cut(s) 1058
PctI GAATGC 1 cut(s) 693
PfeI GAWTC 2 cut(s) 705, 1031
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 8 cut(s) 109, 260, 263, 326, 329, 564, 857, 1074
PshBI ATTAAT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 16
Psp6I CCWGG 3 cut(s) 493, 715, 913
PspFI CCCAGC 1 cut(s) 1089
PspGI CCWGG 3 cut(s) 493, 715, 913
PspN4I GGNNCC 3 cut(s) 141, 250, 1225
PspPI GGNCC 2 cut(s) 163, 539
PsuI RGATCY 3 cut(s) 48, 139, 391
RsaI GTAC 3 cut(s) 41, 589, 974
RsaNI GTAC 3 cut(s) 40, 588, 973
RseI CAYNNNNRTG 3 cut(s) 882, 968, 1221
SacI GAGCTC 1 cut(s) 16
SaqAI TTAA 4 cut(s) 153, 375, 603, 795
SatI GCNGC 8 cut(s) 108, 259, 262, 325, 328, 563, 856, 1073
Sau3AI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
Sau96I GGNCC 2 cut(s) 163, 539
ScaI AGTACT 1 cut(s) 41
ScrFI CCNGG 3 cut(s) 495, 717, 915
SduI GDGCHC 2 cut(s) 16, 1228
SetI ASST 9 cut(s) 16, 180, 420, 499, 544, 595, 727, 816, 1095
SfaNI GCATC 2 cut(s) 217, 574
SinI GGWCC 1 cut(s) 539
SmiMI CAYNNNNRTG 3 cut(s) 882, 968, 1221
SmlI CTYRAG 2 cut(s) 233, 1185
SmoI CTYRAG 2 cut(s) 233, 1185
SphI GCATGC 1 cut(s) 967
Sse9I AATT 2 cut(s) 863, 938
SseBI AGGCCT 1 cut(s) 1058
SsiI CCGC 5 cut(s) 258, 261, 315, 623, 855
SspMI CTAG 1 cut(s) 1109
SstI GAGCTC 1 cut(s) 16
StuI AGGCCT 1 cut(s) 1058
StyD4I CCNGG 3 cut(s) 493, 715, 913
StyI CCWWGG 3 cut(s) 52, 502, 1059
TaaI ACNGT 2 cut(s) 1018, 1050
TasI AATT 2 cut(s) 863, 938
TatI WGTACW 1 cut(s) 39
TauI GCSGC 3 cut(s) 261, 264, 858
TfiI GAWTC 2 cut(s) 705, 1031
Tru1I TTAA 4 cut(s) 153, 375, 603, 795
Tru9I TTAA 4 cut(s) 153, 375, 603, 795
TscAI CASTG 4 cut(s) 1021, 1055, 1074, 1137
TseI GCWGC 5 cut(s) 107, 324, 327, 562, 1072
TspDTI ATGAA 6 cut(s) 500, 530, 744, 972, 1004, 1023
TspRI CASTG 4 cut(s) 1021, 1055, 1074, 1137
VpaK11BI GGWCC 1 cut(s) 539
VspI ATTAAT 1 cut(s) 603
XagI CCTNNNNNAGG 1 cut(s) 842
XceI RCATGY 1 cut(s) 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XspI CTAG 1 cut(s) 1109
ZrmI AGTACT 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.