Rroxscaffold_4G00318430

DnaJ protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000572
Physical Location & Seq
Reverse (-)
44537870 .. 44540846
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318430.1

Sequence Viewer

Length: 1254 bp
ATGTTTGGGAGAGCTCCAAAGAAAAGCGACAACACCAAGTACTATGAGATCCTTGGAGTGCCTAAGAACGCGTCGCAGGATGATCTGAAGAAGGCTTACAGAAAAGCAGCCATCAAGAACCATCCTGATAAGGGTGGGGATCCAGAGAAGTTTAAAGAGTTGGCCCAAGCATACGAGGTTCTGAGTGATCCAGAGAAACGGGAAGTGTATGACCAATATGGCGAGGATGCACTCAAGGAAGGAATGGGTGCCGGAGGCGGCGGCCACGACCCATTTGATATATTCCAGTCCTTCTTTGGAGGCAACCCATTTGGCGGGGGTGGCAGCAGCAGAGGCCGTAGGCAGAGAAGGGGAGAGGATGTGATCCATCCCCTTAAAGTTTCTCTGGAAGATCTATATAATGGGACATCCAAGAAGCTCTCTCTCTCTCGTAACAAAATCTGTGCCAAGTGCAAGGGTAAAGGGTCAAAGTCGGGTGCTTCAATGAAGTGTCCAGGTTGCCAAGGGTCTGGAATGAAAGTCTCTATTAGACATCTTGGACCTTCCATGATCCAGCAAATGCAGCATCCATGCAATGAGTGCAAGGGTACCGGTGAAACCATTAATGACAAGGATCGCTGCCCGCAGTGTAAGGGTGAGAAAGTTGTTCAGGAAAAGAAAGTATTGGAGGTTCATGTGGAGAAGGGCATGCAAAATGGACAGAGAATCACATTCCCTGGTGAAGCTGATGAAGCGCCTGACACCATCACAGGGGATATTGTTTTTGTCCTGCAACAAAAGGAACACCCTAAGTTTAAGCGAAAGGGTGATGACCTATTCTACGAACATATGCTGCCCCTTATGGAGGCACTCTGCGGCTTCCAATTTATCTTCACGCATTTAGATGGCAGGCAACTCCTCATCAAATCTCATCCTGGAGAAGTTGTCAAGCCTGATCAATTCAAGGCTATAAATGATGAAGGCATGCCGATGTACCAGAGGCCATTTATGAAGGGTAAACTATATATACATTTCACTGTGGAGTTCCCAGATTCATTGAAGCCAGAACAGTGCAAGGCCTTGGAAGCAGTGCTGCCGAGGAGTTCTGCCCAGCTTACTGACATGGAACTAGATGAGTGTGAGGAGACTACACTGCATGATGTCAACATAGAGGAGGAAATGCGTCGCAAACAAGCACAGCAGTCTCAAGAGGCATACGACGAGGATGAGGATATGCATGGTGGTGCCCAGAGGGTCCAATGCGCTCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

417

Amino Acids

46.74

Weight (kDa)

6.38

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 13 - 71 1.4e-23 DnaJ domain
DnaJ_C PF01556 123 - 343 5.5e-37 DnaJ C terminal domain
DnaJ_CXXCXGXG PF00684 148 - 214 1.3e-15 DnaJ central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000572)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44110 AT3G44110 AT5G22060
fragaria_vesca FvH4_3g26170 FvH4_3g26170 FvH4_3g26170 FvH4_3g26220 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270 FvH4_7g05270
malus_domestica MD02G1258400.v1.1 MD03G1159100.v1.1 MD07G1062300.v1.1 MD11G1174700.v1.1
prunus_persica Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.2G072900_v2.0.a1 Prupe.6G146900_v2.0.a1 Prupe.6G146900_v2.0.a1
pyrus_communis pycom02g21960 pycom03g11140 pycom07g04780 pycom11g14940
rosa_chinensis RchiOBHm_Chr1g0332041 RchiOBHm_Chr1g0364381 RchiOBHm_Chr4g0428981 RchiOBHm_Chr5g0049001 RchiOBHm_Chr5g0049021
rosa_laevigata RLG00000007108 RLG00000013380 RLG00000027486 RLG00000029697 RLG00000034581 RLG00000034584
rosa_multiflora Rmu_co8449175.1_g000001 Rmu_sc0000441.1_g000032 Rmu_sc0000530.1_g000003 Rmu_sc0003358.1_g000010 Rmu_sc0004058.1_g000007 Rmu_sc0004522.1_g000007 Rmu_sc0008629.1_g000009 Rmu_sc0009489.1_g000007 Rmu_sc0014815.1_g000013
rosa_roxburghii Rroxscaffold_1G00032850 Rroxscaffold_1G00032870 Rroxscaffold_4G00292340 Rroxscaffold_4G00318430 Rroxscaffold_5G00370640
rosa_rugosa Rorug01G0097800 Rorug01G0097900 Rorug01G0098000 Rorug01G0315800 Rorug04G0231300 Rorug05G0241800 Rorug05G0241900 Rorug05G0242000 Rorug05G0242100.1 Rorug05G0242200 Rorug05G0242300 Rorug06G0102700 Rorug06G0102800
rosa_samantha Rh1AG121800 Rh1BG093200 Rh1CG303100 Rh1DG127500 Rh4BG293700 Rh4DG290600 Rh5AG322000 Rh5CG357500 Rh5CG357600 Rh5DG344100 Rh6BG218000
rosa_wichuraiana Rw0G008830 Rw1G009930 Rw1G028700 Rw5G030390 Rw5G030400 Rw6G018630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 587
AccB1I GGYRCC 3 cut(s) 248, 587, 1223
AccII CGCG 1 cut(s) 71
AciI CCGC 5 cut(s) 258, 261, 315, 623, 855
AclWI GGATC 7 cut(s) 43, 134, 147, 182, 358, 544, 621
AcoI YGGCCR 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 3 cut(s) 41, 589, 974
AfiI CCNNNNNNNGG 4 cut(s) 131, 314, 750, 844
AflIII ACRYGT 1 cut(s) 69
AgeI ACCGGT 1 cut(s) 590
AgsI TTSAA 3 cut(s) 483, 943, 1039
AjnI CCWGG 3 cut(s) 493, 715, 913
AluBI AGCT 4 cut(s) 14, 418, 725, 1093
AluI AGCT 4 cut(s) 14, 418, 725, 1093
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 3 cut(s) 526, 1118, 1188
AlwI GGATC 7 cut(s) 43, 134, 147, 182, 358, 544, 621
AoxI GGCC 5 cut(s) 162, 262, 334, 980, 1056
ApeKI GCWGC 7 cut(s) 107, 324, 327, 562, 618, 832, 1072
AseI ATTAAT 1 cut(s) 603
AsiGI ACCGGT 1 cut(s) 590
Asp718I GGTACC 1 cut(s) 587
AspLEI GCGC 2 cut(s) 736, 1244
AspS9I GGNCC 3 cut(s) 163, 539, 1234
AsuHPI GGTGA 4 cut(s) 605, 647, 731, 818
AvaII GGWCC 2 cut(s) 539, 1234
BaeGI GKGCMC 1 cut(s) 1228
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 3 cut(s) 248, 587, 1223
BanII GRGCYC 1 cut(s) 16
BarI GAAGNNNNNNTAC 2 cut(s) 80, 112
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 7 cut(s) 119, 336, 339, 574, 605, 819, 1059
BccI CCATC 5 cut(s) 119, 129, 375, 752, 878
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 3 cut(s) 495, 717, 915
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 3 cut(s) 526, 1118, 1188
BfaI CTAG 1 cut(s) 1109
BfoI RGCGCY 1 cut(s) 737
BglII AGATCT 1 cut(s) 391
BmcAI AGTACT 1 cut(s) 41
Bme1390I CCNGG 3 cut(s) 495, 717, 915
Bme18I GGWCC 2 cut(s) 539, 1234
BmgT120I GGNCC 3 cut(s) 163, 539, 1234
BmiI GGNNCC 5 cut(s) 141, 250, 589, 1225, 1235
BmrFI CCNGG 3 cut(s) 495, 717, 915
BmsI GCATC 2 cut(s) 217, 574
BpmI CTGGAG 1 cut(s) 936
BpuEI CTTGAG 2 cut(s) 218, 1170
BsaJI CCNNGG 5 cut(s) 52, 502, 715, 1059, 1076
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 4 cut(s) 345, 375, 909, 939
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 314, 750, 844
Bse118I RCCGGY 1 cut(s) 590
Bse1I ACTGG 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 580
BseBI CCWGG 3 cut(s) 495, 717, 915
BseDI CCNNGG 5 cut(s) 52, 502, 715, 1059, 1076
BseGI GGATG 9 cut(s) 85, 121, 232, 364, 367, 407, 565, 910, 1210
BseLI CCNNNNNNNGG 4 cut(s) 131, 314, 750, 844
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 4 cut(s) 887, 1093, 1136, 1166
BseSI GKGCMC 1 cut(s) 1228
BseXI GCAGC 7 cut(s) 119, 336, 339, 574, 605, 819, 1059
BseYI CCCAGC 1 cut(s) 1089
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 5 cut(s) 164, 264, 336, 982, 1058
BshNI GGYRCC 3 cut(s) 248, 587, 1223
BshTI ACCGGT 1 cut(s) 590
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 2 cut(s) 252, 591
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 4 cut(s) 131, 314, 750, 844
BsmAI GTCTC 3 cut(s) 526, 1118, 1188
BsmFI GGGAC 1 cut(s) 418
BsnI GGCC 5 cut(s) 164, 264, 336, 982, 1058
Bsp1286I GDGCHC 2 cut(s) 16, 1228
Bsp143I GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
BspACI CCGC 5 cut(s) 258, 261, 315, 623, 855
BspANI GGCC 5 cut(s) 164, 264, 336, 982, 1058
BspCNI CTCAG 1 cut(s) 174
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 5 cut(s) 141, 250, 589, 1225, 1235
BspPI GGATC 7 cut(s) 43, 134, 147, 182, 358, 544, 621
BspT107I GGYRCC 3 cut(s) 248, 587, 1223
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 590
BsrI ACTGG 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 590
BssECI CCNNGG 5 cut(s) 52, 502, 715, 1059, 1076
BssMI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
BssT1I CCWWGG 3 cut(s) 52, 502, 1059
Bst2UI CCWGG 3 cut(s) 495, 717, 915
Bst4CI ACNGT 2 cut(s) 1018, 1050
BstAPI GCANNNNNTGC 1 cut(s) 579
BstC8I GCNNGC 4 cut(s) 623, 689, 890, 965
BstDEI CTNAG 3 cut(s) 63, 182, 789
BstENI CCTNNNNNAGG 1 cut(s) 842
BstF5I GGATG 9 cut(s) 85, 121, 232, 364, 367, 407, 565, 910, 1210
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 1 cut(s) 737
BstHHI GCGC 2 cut(s) 736, 1244
BstKTI GATC 9 cut(s) 51, 85, 142, 190, 366, 394, 552, 616, 937
BstMAI GTCTC 3 cut(s) 526, 1118, 1188
BstMBI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
BstMWI GCNNNNNNNGC 6 cut(s) 321, 333, 562, 579, 731, 1064
BstNI CCWGG 3 cut(s) 495, 717, 915
BstNSI RCATGY 2 cut(s) 691, 967
BstSCI CCNGG 3 cut(s) 493, 715, 913
BstSLI GKGCMC 1 cut(s) 1228
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 7 cut(s) 119, 336, 339, 574, 605, 819, 1059
BstX2I RGATCY 3 cut(s) 48, 139, 391
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 3 cut(s) 48, 139, 391
BsuRI GGCC 5 cut(s) 164, 264, 336, 982, 1058
BtsCI GGATG 9 cut(s) 85, 121, 232, 364, 367, 407, 565, 910, 1210
BtsI GCAGTG 3 cut(s) 632, 1074, 1130
BtsIMutI CAGTG 5 cut(s) 632, 1014, 1055, 1074, 1130
Cac8I GCNNGC 4 cut(s) 623, 689, 890, 965
CfoI GCGC 2 cut(s) 736, 1244
Cfr10I RCCGGY 1 cut(s) 590
Cfr13I GGNCC 3 cut(s) 163, 539, 1234
CseI GACGC 2 cut(s) 60, 1151
Csp6I GTAC 3 cut(s) 40, 588, 973
CspAI ACCGGT 1 cut(s) 590
CviAII CATG 8 cut(s) 547, 570, 674, 688, 964, 1102, 1136, 1217
CviQI GTAC 3 cut(s) 40, 588, 973
DdeI CTNAG 3 cut(s) 63, 182, 789
DpnI GATC 9 cut(s) 50, 84, 141, 189, 365, 393, 551, 615, 936
DpnII GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
DraI TTTAAA 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 262
Ecl136II GAGCTC 1 cut(s) 14
Eco130I CCWWGG 3 cut(s) 52, 502, 1059
Eco147I AGGCCT 1 cut(s) 1058
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 2 cut(s) 539, 1234
Eco53kI GAGCTC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 14
EcoNI CCTNNNNNAGG 1 cut(s) 842
EcoRII CCWGG 3 cut(s) 493, 715, 913
EcoT14I CCWWGG 3 cut(s) 52, 502, 1059
EcoT22I ATGCAT 1 cut(s) 1218
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 3 cut(s) 52, 502, 1059
FaeI CATG 8 cut(s) 550, 573, 677, 691, 967, 1105, 1139, 1220
FaqI GGGAC 1 cut(s) 418
FatI CATG 8 cut(s) 546, 569, 673, 687, 963, 1101, 1135, 1216
FauI CCCGC 2 cut(s) 308, 630
FauNDI CATATG 1 cut(s) 828
FbaI TGATCA 1 cut(s) 934
FokI GGATG 9 cut(s) 92, 108, 239, 354, 371, 394, 552, 897, 1217
FriOI GRGCYC 1 cut(s) 16
FspBI CTAG 1 cut(s) 1109
GlaI GCGC 2 cut(s) 735, 1243
GsaI CCCAGC 1 cut(s) 1093
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 737
HaeIII GGCC 5 cut(s) 164, 264, 336, 982, 1058
HapII CCGG 2 cut(s) 252, 591
HgaI GACGC 2 cut(s) 60, 1151
HhaI GCGC 2 cut(s) 736, 1244
Hin1II CATG 8 cut(s) 550, 573, 677, 691, 967, 1105, 1139, 1220
Hin6I GCGC 2 cut(s) 734, 1242
HinP1I GCGC 2 cut(s) 734, 1242
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HinfI GANTC 2 cut(s) 705, 1031
HpaII CCGG 2 cut(s) 252, 591
HphI GGTGA 4 cut(s) 605, 647, 731, 818
Hpy166II GTNNAC 2 cut(s) 998, 1144
Hpy188I TCNGA 2 cut(s) 87, 183
Hpy188III TCNNGA 8 cut(s) 115, 125, 143, 191, 386, 510, 650, 1187
Hpy8I GTNNAC 2 cut(s) 998, 1144
Hpy99I CGWCG 3 cut(s) 76, 1167, 1202
HpyAV CCTTC 8 cut(s) 85, 233, 301, 342, 552, 676, 953, 985
HpyCH4III ACNGT 2 cut(s) 1018, 1050
HpyF10VI GCNNNNNNNGC 6 cut(s) 321, 333, 562, 579, 731, 1064
HpyF3I CTNAG 3 cut(s) 63, 182, 789
Hsp92II CATG 8 cut(s) 550, 573, 677, 691, 967, 1105, 1139, 1220
HspAI GCGC 2 cut(s) 734, 1242
KpnI GGTACC 1 cut(s) 591
Ksp22I TGATCA 1 cut(s) 934
Kzo9I GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 7 cut(s) 119, 336, 339, 574, 605, 819, 1059
LweI GCATC 2 cut(s) 217, 574
MaeI CTAG 1 cut(s) 1109
MaeIII GTNAC 1 cut(s) 431
MalI GATC 9 cut(s) 50, 84, 141, 189, 365, 393, 551, 615, 936
MboI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
MboII GAAGA 3 cut(s) 100, 401, 862
MflI RGATCY 3 cut(s) 48, 139, 391
MhlI GDGCHC 2 cut(s) 16, 1228
MluCI AATT 2 cut(s) 863, 938
MluI ACGCGT 1 cut(s) 69
Mph1103I ATGCAT 1 cut(s) 1218
MseI TTAA 4 cut(s) 153, 375, 603, 795
MslI CAYNNNNRTG 3 cut(s) 882, 968, 1221
MspI CCGG 2 cut(s) 252, 591
MspR9I CCNGG 3 cut(s) 495, 717, 915
MvaI CCWGG 3 cut(s) 495, 717, 915
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 6 cut(s) 321, 333, 562, 579, 731, 1064
NdeI CATATG 1 cut(s) 828
NdeII GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
NlaIII CATG 8 cut(s) 550, 573, 677, 691, 967, 1105, 1139, 1220
NlaIV GGNNCC 5 cut(s) 141, 250, 589, 1225, 1235
NmeAIII GCCGAG 1 cut(s) 1101
NsiI ATGCAT 1 cut(s) 1218
NspI RCATGY 2 cut(s) 691, 967
PaeI GCATGC 2 cut(s) 691, 967
PceI AGGCCT 1 cut(s) 1058
PfeI GAWTC 2 cut(s) 705, 1031
PfoI TCCNGGA 1 cut(s) 913
PinAI ACCGGT 1 cut(s) 590
PshBI ATTAAT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 16
Psp6I CCWGG 3 cut(s) 493, 715, 913
PspFI CCCAGC 1 cut(s) 1089
PspGI CCWGG 3 cut(s) 493, 715, 913
PspN4I GGNNCC 5 cut(s) 141, 250, 589, 1225, 1235
PspPI GGNCC 3 cut(s) 163, 539, 1234
PsrI GAACNNNNNNTAC 2 cut(s) 654, 686
PsuI RGATCY 3 cut(s) 48, 139, 391
RsaI GTAC 3 cut(s) 41, 589, 974
RsaNI GTAC 3 cut(s) 40, 588, 973
RseI CAYNNNNRTG 3 cut(s) 882, 968, 1221
SacI GAGCTC 1 cut(s) 16
SaqAI TTAA 4 cut(s) 153, 375, 603, 795
Sau3AI GATC 9 cut(s) 48, 82, 139, 187, 363, 391, 549, 613, 934
Sau96I GGNCC 3 cut(s) 163, 539, 1234
ScaI AGTACT 1 cut(s) 41
ScrFI CCNGG 3 cut(s) 495, 717, 915
SduI GDGCHC 2 cut(s) 16, 1228
SetI ASST 9 cut(s) 16, 180, 420, 499, 544, 672, 727, 816, 1095
SfaNI GCATC 2 cut(s) 217, 574
SinI GGWCC 2 cut(s) 539, 1234
SmiMI CAYNNNNRTG 3 cut(s) 882, 968, 1221
SmlI CTYRAG 2 cut(s) 233, 1185
SmoI CTYRAG 2 cut(s) 233, 1185
SphI GCATGC 2 cut(s) 691, 967
Sse9I AATT 2 cut(s) 863, 938
SseBI AGGCCT 1 cut(s) 1058
SsiI CCGC 5 cut(s) 258, 261, 315, 623, 855
SspMI CTAG 1 cut(s) 1109
SstI GAGCTC 1 cut(s) 16
StuI AGGCCT 1 cut(s) 1058
StyD4I CCNGG 3 cut(s) 493, 715, 913
StyI CCWWGG 3 cut(s) 52, 502, 1059
TaaI ACNGT 2 cut(s) 1018, 1050
TasI AATT 2 cut(s) 863, 938
TatI WGTACW 1 cut(s) 39
TauI GCSGC 3 cut(s) 261, 264, 858
TfiI GAWTC 2 cut(s) 705, 1031
Tru1I TTAA 4 cut(s) 153, 375, 603, 795
Tru9I TTAA 4 cut(s) 153, 375, 603, 795
TscAI CASTG 5 cut(s) 632, 1021, 1055, 1074, 1137
TseI GCWGC 7 cut(s) 107, 324, 327, 562, 618, 832, 1072
TspDTI ATGAA 7 cut(s) 500, 530, 662, 744, 972, 1004, 1023
TspRI CASTG 5 cut(s) 632, 1021, 1055, 1074, 1137
VpaK11BI GGWCC 2 cut(s) 539, 1234
VspI ATTAAT 1 cut(s) 603
XagI CCTNNNNNAGG 1 cut(s) 842
XceI RCATGY 2 cut(s) 691, 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XspI CTAG 1 cut(s) 1109
ZrmI AGTACT 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.