MD04G1014300.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001472
Physical Location & Seq
Reverse (-)
1599936 .. 1601488
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1014300.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGAAGAGGATAACCACAAGACCTCCATCTCCAGGTAATCTTCCTTCTTTCTTTTTTACCAGTCTGCAGAATACAAGTAGGGGATTTCATTCCAATGCTGTTCATGCTAATCCTACTTGTAAGGATAGAATTAAACAAACCCATTTGGTGCCGGAGAGTACGATCCAAACTCGATGCAAATCTGGAAAAATTAGGAAAGATGAAGCATTGGGTTACTTTAATTCTATGATTCAAACCAAACCCTTGCCCTCGATTTGGACTTTCAATTGTTTGTTTGGGGCACTCTCCAAGATGAAGCAATATTCTGCTGTGGTTTCTATGTGTAAACAGTTGATGGATTGCACTCAATTCCGACCTGAGGTAGGTACAATGAATATTTTTCTGAATTGCTTGTGCCGGCTGAATCGGGTGAACGCTTGTTTCTCTGTTTTAGCAATTACCCTTAAATATGGTCTTAAGCCCGATGCATGTTCTATAAATACTTTGCTTCACGGGCTTTGGAAGTACAGTTCCCTGGCCGAGGCAATGGAGTTCTTCCAGGAAATAGAAACGAAAGGATACAAATGCAATGAAATCACTTGTGCTACCATGATCAATGGACTATGCAAAGCTGGGAAAACTTCTAAGGCGCTCGAGATACTTACGAAGATGTGGGAAGGTGGAAGGTTCAACCCCAACCAAGAATGCTATAATCCAATCATCGATAGCCTCTGTAAAGAAAGACGAATAGACGAGGCCTTGACCCTATTTCGCGATATGATCAGCAAGAGTGTTGTACCAAATATTTTCAATTATACCACATTGATTCATGGGTTATGCAACACAAGTCGCTGGGAAGAAGTGCTCTCTCTTTTTGAAGGAATGGTAGATCAAGGAGTCAAGCCTAATGTTGTCACCTACAACACTCTAATTTATGGACTGTGCCAATCGGGGAGGTATGAAAAAGCCAAGAGCTTCTTGATTTGCATGCTTAACAGTGGAATTTCACCCGACGTATACACCTATAATGTTCTGCTGAACACTCTTTGCAAGGAAGAAAGAATTCAAGAAGCACTTACTCTGTTTGGAACAATGACTGAGAAAGGTATAAAGCCTGATGTTTTCACTTTTAATTCCATGATTTCTGCTTCATGCAAGTCTGGTAACTGGGACGAAGGTGTGAGGTTATTTAAAAACATGATTGATTATGGAGTCTTGCCTGATATTGTTACATACAATGCTGTTCTGGATGCTTTATGCAAGGAAGGGAGGACAGAAGAGGCACTGAATCTTGTGGAAGAAATGGTCCGCAGAGGTGAAAGGCCTAATTGTGTGACTTACAACTCCTTAATTAATGGATTGTGCCGGACCAGCCAATGGAGAGAAGCCACAAGGTTGTTCGATGAAATGGTTTGCCATGGAATCTTACCTAATTTCATAACTTTGAAGATACTCTCCGGTGCTCTCTGCAATGGAAGGATGACAGATGAGGTTCGCAAAGAATTTGAAGATAGATTAGATGGAGCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

504

Amino Acids

56.9

Weight (kDa)

8.49

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 119 - 166 4.5e-07 PPR repeat family
PPR_2 PF13041 154 - 203 4.6e-10 PPR repeat family
PPR_long PF17177 170 - 269 1.7e-06 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 175 - 286 7.3e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 185 - 217 3.4e-07 PPR repeat
PPR_1 PF12854 224 - 253 2e-08 PPR repeat
PPR_2 PF13041 225 - 274 1.5e-14 PPR repeat family
PPR PF01535 229 - 257 6.8e-06 PPR repeat
PPR_long PF17177 245 - 381 9.7e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 249 - 304 5e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 260 - 309 4.3e-17 PPR repeat family
PPR_3 PF13812 283 - 342 5.8e-12 Pentatricopeptide repeat domain
TPR_24 PF23276 291 - 393 1.4e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 292 - 323 1.3e-11 PPR repeat
PPR_2 PF13041 295 - 344 2.4e-18 PPR repeat family
PPR PF01535 298 - 328 1e-07 PPR repeat
PPR_3 PF13812 323 - 376 4.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 327 - 358 1.9e-09 PPR repeat
PPR_long PF17177 331 - 434 3e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 334 - 379 8.9e-15 PPR repeat family
PPR_3 PF13812 354 - 412 7.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 361 - 393 4.3e-11 PPR repeat
PPR_2 PF13041 365 - 413 1.7e-15 PPR repeat family
PPR PF01535 368 - 398 6.9e-08 PPR repeat
MRP-S27 PF10037 369 - 434 6.2e-07 Mitochondrial 28S ribosomal protein S27
PPR_1 PF12854 397 - 429 9e-12 PPR repeat
PPR_2 PF13041 400 - 449 1.6e-20 PPR repeat family
PPR PF01535 403 - 432 4.2e-09 PPR repeat
PPR_3 PF13812 424 - 476 5.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 432 - 464 8.2e-12 PPR repeat
PPR_2 PF13041 435 - 484 5.8e-15 PPR repeat family
PPR PF01535 438 - 468 3.5e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 148
AccB7I CCANNNNNTGG 1 cut(s) 1356
AccI GTMKAC 1 cut(s) 996
AccII CGCG 1 cut(s) 753
AciI CCGC 1 cut(s) 1288
AclWI GGATC 1 cut(s) 157
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 3 cut(s) 981, 1041, 1481
AfaI GTAC 4 cut(s) 160, 367, 506, 777
AfiI CCNNNNNNNGG 6 cut(s) 32, 255, 358, 362, 520, 1356
AflII CTTAAG 1 cut(s) 455
AgsI TTSAA 8 cut(s) 233, 265, 670, 790, 857, 1046, 1426, 1487
AjnI CCWGG 3 cut(s) 31, 513, 537
AloI GAACNNNNNNTCC 2 cut(s) 493, 525
AluBI AGCT 3 cut(s) 611, 954, 1505
AluI AGCT 3 cut(s) 611, 954, 1505
Alw21I GWGCWC 2 cut(s) 846, 1444
AlwI GGATC 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 3 cut(s) 516, 735, 1301
ApoI RAATTY 3 cut(s) 981, 1041, 1481
AseI ATTAAT 1 cut(s) 1332
Asp700I GAANNNNTTC 1 cut(s) 1041
AspLEI GCGC 1 cut(s) 631
AspS9I GGNCC 2 cut(s) 1285, 1347
AsuHPI GGTGA 4 cut(s) 421, 886, 978, 1307
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 2 cut(s) 1285, 1347
AxyI CCTNAGG 1 cut(s) 357
BaeGI GKGCMC 1 cut(s) 283
BanI GGYRCC 1 cut(s) 148
BarI GAAGNNNNNNTAC 2 cut(s) 28, 60
Bbv12I GWGCWC 2 cut(s) 846, 1444
BccI CCATC 3 cut(s) 34, 328, 1493
BciT130I CCWGG 3 cut(s) 33, 515, 539
BciVI GTATCC 1 cut(s) 551
BclI TGATCA 2 cut(s) 591, 759
BfmI CTRYAG 1 cut(s) 65
BfoI RGCGCY 1 cut(s) 632
BfrI CTTAAG 1 cut(s) 455
BfuI GTATCC 1 cut(s) 551
Bme1390I CCNGG 3 cut(s) 33, 515, 539
Bme18I GGWCC 2 cut(s) 1285, 1347
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 1285, 1347
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 3 cut(s) 33, 515, 539
BmrI ACTGGG 1 cut(s) 1156
BmsI GCATC 3 cut(s) 164, 454, 1219
BmuI ACTGGG 1 cut(s) 1156
BpmI CTGGAG 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 702
BsaBI GATNNNNATC 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 513, 519, 1396
BsaWI WCCGGW 1 cut(s) 1436
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 255, 358, 362, 520, 1356
Bse118I RCCGGY 1 cut(s) 396
Bse1I ACTGG 2 cut(s) 60, 1151
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 3 cut(s) 531, 574, 1456
Bse8I GATNNNNATC 1 cut(s) 178
BseBI CCWGG 3 cut(s) 33, 515, 539
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 3 cut(s) 513, 519, 1396
BseGI GGATG 2 cut(s) 1234, 1464
BseJI GATNNNNATC 1 cut(s) 178
BseLI CCNNNNNNNGG 6 cut(s) 32, 255, 358, 362, 520, 1356
BseMI GCAATG 3 cut(s) 531, 574, 1456
BseMII CTCAG 2 cut(s) 348, 1068
BseNI ACTGG 2 cut(s) 60, 1151
BseSI GKGCMC 1 cut(s) 283
BseYI CCCAGC 2 cut(s) 611, 831
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 3 cut(s) 518, 737, 1303
BshNI GGYRCC 1 cut(s) 148
BshVI ATCGAT 1 cut(s) 702
BsiHKAI GWGCWC 2 cut(s) 846, 1444
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 4 cut(s) 152, 397, 1345, 1437
BslFI GGGAC 1 cut(s) 1163
BslI CCNNNNNNNGG 6 cut(s) 32, 255, 358, 362, 520, 1356
BsmFI GGGAC 1 cut(s) 1163
BsmI GAATGC 1 cut(s) 689
BsnI GGCC 3 cut(s) 518, 737, 1303
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 3 cut(s) 283, 846, 1444
Bsp143I GATC 4 cut(s) 162, 591, 759, 868
Bsp19I CCATGG 1 cut(s) 1396
Bsp68I TCGCGA 1 cut(s) 753
BspACI CCGC 1 cut(s) 1288
BspANI GGCC 3 cut(s) 518, 737, 1303
BspCNI CTCAG 2 cut(s) 349, 1069
BspDI ATCGAT 1 cut(s) 702
BspFNI CGCG 1 cut(s) 753
BspLI GGNNCC 1 cut(s) 150
BspMAI CTGCAG 1 cut(s) 69
BspPI GGATC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 148
BspTI CTTAAG 1 cut(s) 455
BsrDI GCAATG 3 cut(s) 531, 574, 1456
BsrFI RCCGGY 1 cut(s) 396
BsrI ACTGG 2 cut(s) 60, 1151
BssAI RCCGGY 1 cut(s) 396
BssECI CCNNGG 3 cut(s) 513, 519, 1396
BssMI GATC 4 cut(s) 162, 591, 759, 868
BssNAI GTATAC 1 cut(s) 997
BssT1I CCWWGG 1 cut(s) 1396
Bst1107I GTATAC 1 cut(s) 997
Bst2UI CCWGG 3 cut(s) 33, 515, 539
Bst4CI ACNGT 4 cut(s) 330, 509, 921, 977
Bst6I CTCTTC 1 cut(s) 1251
BstAFI CTTAAG 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 398, 968
BstDEI CTNAG 3 cut(s) 357, 624, 1077
BstDSI CCRYGG 1 cut(s) 1396
BstENI CCTNNNNNAGG 2 cut(s) 360, 518
BstF5I GGATG 2 cut(s) 1234, 1464
BstFNI CGCG 1 cut(s) 753
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 4 cut(s) 165, 594, 762, 871
BstMBI GATC 4 cut(s) 162, 591, 759, 868
BstMWI GCNNNNNNNGC 3 cut(s) 104, 493, 1350
BstNI CCWGG 3 cut(s) 33, 515, 539
BstNSI RCATGY 2 cut(s) 471, 970
BstSCI CCNGG 3 cut(s) 31, 513, 537
BstSFI CTRYAG 1 cut(s) 65
BstSLI GKGCMC 1 cut(s) 283
BstUI CGCG 1 cut(s) 753
BstZ17I GTATAC 1 cut(s) 997
Bsu15I ATCGAT 1 cut(s) 702
Bsu36I CCTNAGG 1 cut(s) 357
BsuI GTATCC 1 cut(s) 551
BsuRI GGCC 3 cut(s) 518, 737, 1303
BsuTUI ATCGAT 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 1396
BtsCI GGATG 2 cut(s) 1234, 1464
BtsIMutI CAGTG 2 cut(s) 982, 1262
BtuMI TCGCGA 1 cut(s) 753
Cac8I GCNNGC 2 cut(s) 398, 968
CfoI GCGC 1 cut(s) 631
Cfr10I RCCGGY 1 cut(s) 396
Cfr13I GGNCC 2 cut(s) 1285, 1347
ClaI ATCGAT 1 cut(s) 702
Csp6I GTAC 4 cut(s) 159, 366, 505, 776
CviAII CATG 9 cut(s) 104, 468, 589, 809, 967, 1117, 1131, 1177, 1397
CviQI GTAC 4 cut(s) 159, 366, 505, 776
DdeI CTNAG 3 cut(s) 357, 624, 1077
DpnI GATC 4 cut(s) 164, 593, 761, 870
DpnII GATC 4 cut(s) 162, 591, 759, 868
DraI TTTAAA 1 cut(s) 1171
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 1 cut(s) 1251
EarI CTCTTC 1 cut(s) 1251
Eco130I CCWWGG 1 cut(s) 1396
Eco147I AGGCCT 2 cut(s) 737, 1303
Eco47I GGWCC 2 cut(s) 1285, 1347
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 632
EcoNI CCTNNNNNAGG 2 cut(s) 360, 518
EcoRI GAATTC 1 cut(s) 1041
EcoRII CCWGG 3 cut(s) 31, 513, 537
EcoT14I CCWWGG 1 cut(s) 1396
EcoT22I ATGCAT 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 1396
FaeI CATG 9 cut(s) 107, 471, 592, 812, 970, 1120, 1134, 1180, 1400
FalI AAGNNNNNCTT 4 cut(s) 941, 973, 1038, 1070
FaqI GGGAC 1 cut(s) 1163
FatI CATG 9 cut(s) 103, 467, 588, 808, 966, 1116, 1130, 1176, 1396
FbaI TGATCA 2 cut(s) 591, 759
FblI GTMKAC 1 cut(s) 996
FokI GGATG 2 cut(s) 1241, 1471
GlaI GCGC 1 cut(s) 630
GsaI CCCAGC 2 cut(s) 615, 835
GsuI CTGGAG 1 cut(s) 15
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 3 cut(s) 518, 737, 1303
HapII CCGG 4 cut(s) 152, 397, 1345, 1437
HhaI GCGC 1 cut(s) 631
Hin1II CATG 9 cut(s) 107, 471, 592, 812, 970, 1120, 1134, 1180, 1400
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HinfI GANTC 7 cut(s) 229, 403, 805, 876, 1191, 1267, 1401
HpaII CCGG 4 cut(s) 152, 397, 1345, 1437
HphI GGTGA 4 cut(s) 421, 886, 978, 1307
Hpy166II GTNNAC 3 cut(s) 326, 412, 997
Hpy188I TCNGA 2 cut(s) 353, 384
Hpy188III TCNNGA 6 cut(s) 183, 634, 752, 958, 1046, 1226
Hpy8I GTNNAC 3 cut(s) 326, 412, 997
Hpy99I CGWCG 1 cut(s) 995
HpyAV CCTTC 7 cut(s) 54, 650, 657, 851, 1148, 1238, 1449
HpyCH4III ACNGT 4 cut(s) 330, 509, 921, 977
HpyCH4IV ACGT 1 cut(s) 993
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 493, 1350
HpyF3I CTNAG 3 cut(s) 357, 624, 1077
HpySE526I ACGT 1 cut(s) 993
Hsp92II CATG 9 cut(s) 107, 471, 592, 812, 970, 1120, 1134, 1180, 1400
HspAI GCGC 1 cut(s) 629
KroI GCCGGC 1 cut(s) 396
KroNI GCCGGC 1 cut(s) 398
Ksp22I TGATCA 2 cut(s) 591, 759
Kzo9I GATC 4 cut(s) 162, 591, 759, 868
LmnI GCTCC 1 cut(s) 1502
LweI GCATC 3 cut(s) 164, 454, 1219
MaeII ACGT 1 cut(s) 993
MaeIII GTNAC 5 cut(s) 212, 892, 1142, 1207, 1312
MalI GATC 4 cut(s) 164, 593, 761, 870
MboI GATC 4 cut(s) 162, 591, 759, 868
MfeI CAATTG 1 cut(s) 265
MhlI GDGCHC 3 cut(s) 283, 846, 1444
MlyI GAGTC 2 cut(s) 885, 1200
MmeI TCCRAC 1 cut(s) 376
Mph1103I ATGCAT 1 cut(s) 469
MroNI GCCGGC 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 1041
MseI TTAA 9 cut(s) 132, 219, 444, 456, 972, 1110, 1170, 1328, 1332
MslI CAYNNNNRTG 1 cut(s) 93
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 4 cut(s) 152, 397, 1345, 1437
MspR9I CCNGG 3 cut(s) 33, 515, 539
MunI CAATTG 1 cut(s) 265
Mva1269I GAATGC 1 cut(s) 689
MvaI CCWGG 3 cut(s) 33, 515, 539
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 3 cut(s) 104, 493, 1350
NaeI GCCGGC 1 cut(s) 398
NcoI CCATGG 1 cut(s) 1396
NdeII GATC 4 cut(s) 162, 591, 759, 868
NgoMIV GCCGGC 1 cut(s) 396
NlaIII CATG 9 cut(s) 107, 471, 592, 812, 970, 1120, 1134, 1180, 1400
NlaIV GGNNCC 1 cut(s) 150
NmeAIII GCCGAG 1 cut(s) 544
NmuCI GTSAC 2 cut(s) 892, 1312
NruI TCGCGA 1 cut(s) 753
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 2 cut(s) 471, 970
PacI TTAATTAA 1 cut(s) 1332
PaeI GCATGC 1 cut(s) 970
PaeR7I CTCGAG 1 cut(s) 632
PceI AGGCCT 2 cut(s) 737, 1303
PctI GAATGC 1 cut(s) 689
PdiI GCCGGC 1 cut(s) 398
PdmI GAANNNNTTC 1 cut(s) 1041
PfeI GAWTC 5 cut(s) 229, 403, 805, 1267, 1401
PflMI CCANNNNNTGG 1 cut(s) 1356
PfoI TCCNGGA 1 cut(s) 537
PleI GAGTC 2 cut(s) 884, 1199
PpsI GAGTC 2 cut(s) 884, 1199
PshBI ATTAAT 1 cut(s) 1332
Psp6I CCWGG 3 cut(s) 31, 513, 537
PspFI CCCAGC 2 cut(s) 611, 831
PspGI CCWGG 3 cut(s) 31, 513, 537
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 2 cut(s) 1285, 1347
PstI CTGCAG 1 cut(s) 69
RruI TCGCGA 1 cut(s) 753
RsaI GTAC 4 cut(s) 160, 367, 506, 777
RsaNI GTAC 4 cut(s) 159, 366, 505, 776
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 9 cut(s) 132, 219, 444, 456, 972, 1110, 1170, 1328, 1332
Sau3AI GATC 4 cut(s) 162, 591, 759, 868
Sau96I GGNCC 2 cut(s) 1285, 1347
SchI GAGTC 2 cut(s) 885, 1200
ScrFI CCNGG 3 cut(s) 33, 515, 539
SduI GDGCHC 3 cut(s) 283, 846, 1444
SfaNI GCATC 3 cut(s) 164, 454, 1219
SfcI CTRYAG 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 632
SinI GGWCC 2 cut(s) 1285, 1347
SlaI CTCGAG 1 cut(s) 632
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 2 cut(s) 455, 632
SmoI CTYRAG 2 cut(s) 455, 632
SphI GCATGC 1 cut(s) 970
SseBI AGGCCT 2 cut(s) 737, 1303
SsiI CCGC 1 cut(s) 1288
SspI AATATT 3 cut(s) 302, 376, 784
StuI AGGCCT 2 cut(s) 737, 1303
StyD4I CCNGG 3 cut(s) 31, 513, 537
StyI CCWWGG 1 cut(s) 1396
TaaI ACNGT 4 cut(s) 330, 509, 921, 977
TaiI ACGT 1 cut(s) 996
TaqI TCGA 5 cut(s) 172, 251, 633, 702, 1380
TatI WGTACW 1 cut(s) 504
TfiI GAWTC 5 cut(s) 229, 403, 805, 1267, 1401
Tru1I TTAA 9 cut(s) 132, 219, 444, 456, 972, 1110, 1170, 1328, 1332
Tru9I TTAA 9 cut(s) 132, 219, 444, 456, 972, 1110, 1170, 1328, 1332
TscAI CASTG 2 cut(s) 982, 1269
TseFI GTSAC 2 cut(s) 892, 1312
Tsp45I GTSAC 2 cut(s) 892, 1312
TspRI CASTG 2 cut(s) 982, 1269
Van91I CCANNNNNTGG 1 cut(s) 1356
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 1285, 1347
VspI ATTAAT 1 cut(s) 1332
XagI CCTNNNNNAGG 2 cut(s) 360, 518
XapI RAATTY 3 cut(s) 981, 1041, 1481
XceI RCATGY 2 cut(s) 471, 970
XhoI CTCGAG 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 1 cut(s) 1041
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.