Rroxscaffold_5G00358060

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001472
Physical Location & Seq
Reverse (-)
38027559 .. 38035047
7489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00358060.1

Sequence Viewer

Length: 672 bp
ATGAAGCCGAAGCAATGCTCTAAAGCTGGAGCCCAGTTTGGGACCAAACCTGAGCACTTGCCGAACCAAGACTCTCTTGCTGAAGCAATGGAGCTGTTCCGGAAAATACAACAGAAGAGTTACACATGTAATGAGTTCACCTATGCTATCATTATTAATGGGTTGTGCAAAGCTGGGAAGACTAATGATGGCATCAAGATACTTAAGCGGATGCATAAGGATGGAAGGTTTAAGCCGGATCCGGGTTGCTACAATCCAATCACTGACAGCCTTTTTAAAGAAAGACGAATAGATGAGGTCTTGACCCTATTTCAAGAGATGAGCAATGCTGGGGAAACTACTAATGGATTATGCAACATGGGTTATTGGGATAGTGTACTCACTCTGTTTGAAACAATGAAGGATCGAGGAACCACCCCAAATGCTGTCACCTTCAGCACCCTAATATATAGACTTTGCCGATCAGGCAAGTGGGAAAAAAATGCCCAGAAATTCTTGTCTTGCATGGTTGATAGTGGAATTTCCCCAAATGCATATACCTACAATGCCCTAATCAGCAGTAATTGCAAGGAAGGAAAAGTCAAAGAAGCACTCTCTCTGTTTGAATACATGACAATGAAAGGCATAAAGCCTAGTGTTGCCACTTTCGATTACTTAAGCCTCGTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

223

Amino Acids

25.13

Weight (kDa)

8.92

Isoelectric Point (pI)

24.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 24 - 57 3.6e-07 PPR repeat family
PPR_1 PF12854 42 - 71 1.8e-08 PPR repeat
PPR_2 PF13041 115 - 154 4.5e-10 PPR repeat family
PPR_long PF17177 124 - 212 3.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 137 - 169 3.1e-06 PPR repeat
PPR_2 PF13041 147 - 190 2.3e-08 PPR repeat family
PPR_3 PF13812 168 - 219 8.5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 173 - 205 1.8e-12 PPR repeat
PPR PF01535 179 - 209 5.4e-09 PPR repeat
PPR_2 PF13041 180 - 218 2.4e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 99
AciI CCGC 1 cut(s) 208
AclWI GGATC 3 cut(s) 233, 246, 411
AcsI RAATTY 2 cut(s) 491, 519
AcuI CTGAAG 2 cut(s) 102, 418
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 2 cut(s) 39, 242
AflII CTTAAG 2 cut(s) 203, 655
AflIII ACRYGT 1 cut(s) 125
AgsI TTSAA 3 cut(s) 314, 392, 605
AluBI AGCT 3 cut(s) 26, 94, 173
AluI AGCT 3 cut(s) 26, 94, 173
Alw21I GWGCWC 1 cut(s) 57
AlwI GGATC 3 cut(s) 233, 246, 411
Aor13HI TCCGGA 1 cut(s) 99
ApoI RAATTY 2 cut(s) 491, 519
AseI ATTAAT 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 2 cut(s) 130, 421
AvaII GGWCC 1 cut(s) 42
BamHI GGATCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 34
BauI CACGAG 1 cut(s) 662
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 57
BccI CCATC 2 cut(s) 182, 215
BcnI CCSGG 1 cut(s) 243
BfaI CTAG 1 cut(s) 633
BfrI CTTAAG 2 cut(s) 203, 655
BglI GCCNNNNNGGC 1 cut(s) 465
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 4 cut(s) 31, 43, 240, 412
BmrFI CCNGG 1 cut(s) 243
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 2 cut(s) 201, 201
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 48
Bpu10I CCTNAGC 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 243
BsaWI WCCGGW 1 cut(s) 99
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 242
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 3 cut(s) 20, 93, 331
BseAI TCCGGA 1 cut(s) 99
BseGI GGATG 2 cut(s) 216, 226
BseLI CCNNNNNNNGG 2 cut(s) 39, 242
BseMI GCAATG 3 cut(s) 20, 93, 331
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 34
BseYI CCCAGC 2 cut(s) 173, 329
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 3 cut(s) 100, 236, 242
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 2 cut(s) 39, 242
BsmFI GGGAC 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 34, 57
Bsp13I TCCGGA 1 cut(s) 99
Bsp143I GATC 3 cut(s) 238, 403, 461
BspACI CCGC 1 cut(s) 208
BspCNI CTCAG 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 99
BspLI GGNNCC 4 cut(s) 31, 43, 240, 412
BspPI GGATC 3 cut(s) 233, 246, 411
BspTI CTTAAG 2 cut(s) 203, 655
BsrDI GCAATG 3 cut(s) 20, 93, 331
BsrI ACTGG 1 cut(s) 34
BssMI GATC 3 cut(s) 238, 403, 461
BssSI CACGAG 1 cut(s) 662
Bst2BI CACGAG 1 cut(s) 662
Bst6I CTCTTC 1 cut(s) 110
BstAFI CTTAAG 2 cut(s) 203, 655
BstAPI GCANNNNNTGC 1 cut(s) 564
BstDEI CTNAG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 216, 226
BstKTI GATC 3 cut(s) 241, 406, 464
BstMBI GATC 3 cut(s) 238, 403, 461
BstMWI GCNNNNNNNGC 2 cut(s) 465, 564
BstNSI RCATGY 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BtsCI GGATG 2 cut(s) 216, 226
BtsIMutI CAGTG 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 377
CviAII CATG 4 cut(s) 126, 358, 505, 610
CviJI RGCY 9 cut(s) 7, 26, 32, 94, 173, 235, 270, 631, 660
CviKI_1 RGCY 9 cut(s) 7, 26, 32, 94, 173, 235, 270, 631, 660
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 1 cut(s) 51
DpnI GATC 3 cut(s) 240, 405, 463
DpnII GATC 3 cut(s) 238, 403, 461
DraI TTTAAA 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 102, 418
EcoT22I ATGCAT 2 cut(s) 216, 535
EcoT38I GRGCYC 1 cut(s) 34
FaeI CATG 4 cut(s) 129, 361, 508, 613
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FaqI GGGAC 1 cut(s) 55
FatI CATG 4 cut(s) 125, 357, 504, 609
FokI GGATG 2 cut(s) 223, 233
FriOI GRGCYC 1 cut(s) 34
FspBI CTAG 1 cut(s) 633
GsaI CCCAGC 2 cut(s) 177, 333
GsuI CTGGAG 1 cut(s) 48
HapII CCGG 3 cut(s) 100, 236, 242
Hin1II CATG 4 cut(s) 129, 361, 508, 613
HinfI GANTC 1 cut(s) 71
HpaII CCGG 3 cut(s) 100, 236, 242
HphI GGTGA 2 cut(s) 130, 421
Hpy166II GTNNAC 2 cut(s) 138, 377
Hpy188III TCNNGA 4 cut(s) 100, 196, 301, 314
Hpy8I GTNNAC 2 cut(s) 138, 377
HpyAV CCTTC 4 cut(s) 219, 394, 442, 566
HpyCH4V TGCA 7 cut(s) 168, 214, 354, 504, 533, 567, 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 465, 564
HpyF3I CTNAG 1 cut(s) 51
Hsp92II CATG 4 cut(s) 129, 361, 508, 613
Kpn2I TCCGGA 1 cut(s) 99
Kzo9I GATC 3 cut(s) 238, 403, 461
LmnI GCTCC 2 cut(s) 29, 91
LweI GCATC 2 cut(s) 201, 201
MaeI CTAG 1 cut(s) 633
MaeIII GTNAC 2 cut(s) 119, 427
MalI GATC 3 cut(s) 240, 405, 463
MboI GATC 3 cut(s) 238, 403, 461
MboII GAAGA 2 cut(s) 127, 190
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 34, 57
MluCI AATT 3 cut(s) 491, 519, 562
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 3 cut(s) 289, 401, 671
Mph1103I ATGCAT 2 cut(s) 216, 535
MroI TCCGGA 1 cut(s) 99
MseI TTAA 5 cut(s) 156, 204, 231, 276, 656
MslI CAYNNNNRTG 2 cut(s) 219, 614
MspCI CTTAAG 2 cut(s) 203, 655
MspI CCGG 3 cut(s) 100, 236, 242
MspR9I CCNGG 1 cut(s) 243
MwoI GCNNNNNNNGC 2 cut(s) 465, 564
NciI CCSGG 1 cut(s) 243
NdeII GATC 3 cut(s) 238, 403, 461
NlaIII CATG 4 cut(s) 129, 361, 508, 613
NlaIV GGNNCC 4 cut(s) 31, 43, 240, 412
NmuCI GTSAC 1 cut(s) 427
NsiI ATGCAT 2 cut(s) 216, 535
NspI RCATGY 1 cut(s) 129
PciI ACATGT 1 cut(s) 125
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PscI ACATGT 1 cut(s) 125
PshBI ATTAAT 1 cut(s) 156
PspFI CCCAGC 2 cut(s) 173, 329
PspN4I GGNNCC 4 cut(s) 31, 43, 240, 412
PspPI GGNCC 1 cut(s) 42
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
RseI CAYNNNNRTG 2 cut(s) 219, 614
SaqAI TTAA 5 cut(s) 156, 204, 231, 276, 656
Sau3AI GATC 3 cut(s) 238, 403, 461
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 2 cut(s) 34, 57
SetI ASST 9 cut(s) 28, 52, 96, 143, 175, 230, 300, 434, 542
SfaNI GCATC 2 cut(s) 201, 201
SinI GGWCC 1 cut(s) 42
SmiMI CAYNNNNRTG 2 cut(s) 219, 614
SmlI CTYRAG 2 cut(s) 203, 655
SmoI CTYRAG 2 cut(s) 203, 655
Sse9I AATT 3 cut(s) 491, 519, 562
SsiI CCGC 1 cut(s) 208
SspMI CTAG 1 cut(s) 633
StyD4I CCNGG 1 cut(s) 241
TaqI TCGA 2 cut(s) 406, 648
TasI AATT 3 cut(s) 491, 519, 562
TatI WGTACW 1 cut(s) 376
Tru1I TTAA 5 cut(s) 156, 204, 231, 276, 656
Tru9I TTAA 5 cut(s) 156, 204, 231, 276, 656
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 3 cut(s) 17, 413, 632
TspRI CASTG 1 cut(s) 268
Vha464I CTTAAG 2 cut(s) 203, 655
VpaK11BI GGWCC 1 cut(s) 42
VspI ATTAAT 1 cut(s) 156
XapI RAATTY 2 cut(s) 491, 519
XceI RCATGY 1 cut(s) 129
XspI CTAG 1 cut(s) 633
Zsp2I ATGCAT 2 cut(s) 216, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.