MD04G1015100.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001472
Physical Location & Seq
Reverse (-)
1658010 .. 1659197
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1015100.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGTTGGAAGATGGAAGGTTCAAGCCCAAACAAGACTGCTACAATCCCATCATAGATAGCCTCTGTAAAGAAAGACGAATAGATGAGGCCTTGACCCTATTTAGAGATATGATCTACAAAAGTGTTGTACCGGGTATTATCACTTATAACACTTTGATTCATGGGTTATGCAACATGAATTGCTGGGAAGAAGTGTTCTCTCTTTTTGAAGCAATGGAAGATCAAGGAGTCAGGCCAGATGTTCTCACCTACTCCGCTCTAATTTATGCATTGTGCCAATCGGGGAGGTTGGAAAAAGCCAAGAGCTTCTTGATTTGTATGCTTGACAGTGGAATTTCACCCAGTGTATACACATATACTGTTCTTATCAGCGGTATTTGCAAGGAAGAAAGAATTCAAGAAGCGCTTACTCTGTTTGAAAAGATGACAATGAGAGGCATAAAACCTGATCTTGTCACTTTTAATTGCTTGATTTCTGCTTCATGCAAGTCTGGTAACTGGGAGGAAGGTGTGAGTTTATTTAAAACCATGATTGGTTATGGAGCCTTGCCTAATATTGTTACATATAATTCTGTACTGGATGCTTTATGCAAGGAAGGGAAGACAGCAGAGGCACTGAATCTTGTGGAAGAAATGATCCGTAGAGAGGAAAGGCCTGATGTTGTGACTTACAGCTCCTTAATTAATGGATTGTGCTGGACCAGCCACTGGAGAGAAGCTACAAGGGTGTTTAATAGAATGCTGGATGAAGGTATTGCCCTAAATGTTATCACTTACAACAGTTTAACCAATGGCATGTGCCGTACAGCTCAATGGGAAGAGGCCACAAGGTTGTTTGATGATATGATAGGCCGGGGACTCTTGCCTGATACCGTTACTTTGGTAATACTATTGGATGCTGGGATGACAGAAGAGACTAACAAAATATTTGAGGCAACAATTGAATACGGTATGAAACTCAGCACAATAACTTGCAGTATGCTGGTTAGTGAATATTGTTTGCAGGGCAGAATGGAAAAGGCGAAGGTGGTCTTGAACTTAATGGTGATTATGGGTTATGCGCCTGATATTGCTTCCCATAAAACCTTGGTCAACAGAATCGGTGAAGCTTTGAGGCTTGTTAAAGAAATTATCCAGATAGGATTGATGCCTGATCTGGAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

389

Amino Acids

43.81

Weight (kDa)

4.99

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 9 - 37 2.4e-07 PPR repeat
PPR_2 PF13041 12 - 58 5.3e-14 PPR repeat family
PPR_long PF17177 17 - 166 6.5e-11 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 41 - 73 1.5e-06 PPR repeat
PPR_2 PF13041 44 - 93 4.2e-17 PPR repeat family
PPR PF01535 47 - 77 1.1e-07 PPR repeat
PPR_3 PF13812 67 - 126 6.7e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 69 - 176 2.8e-10 Fungal tetratrico peptide repeats
PPR_1 PF12854 76 - 107 1.5e-08 PPR repeat
PPR PF01535 83 - 112 4.1e-06 PPR repeat
PPR_2 PF13041 86 - 128 5.3e-11 PPR repeat family
PPR_1 PF12854 111 - 143 3.2e-10 PPR repeat
PPR PF01535 117 - 147 7.8e-07 PPR repeat
PPR_2 PF13041 119 - 163 2.2e-13 PPR repeat family
PPR_long PF17177 130 - 216 1.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 138 - 196 7.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 145 - 177 8.8e-10 PPR repeat
PPR_2 PF13041 149 - 198 9.6e-15 PPR repeat family
PPR PF01535 152 - 181 4e-07 PPR repeat
PPR_1 PF12854 181 - 212 9.9e-11 PPR repeat
PPR_2 PF13041 184 - 232 1.8e-18 PPR repeat family
PPR PF01535 187 - 215 1.4e-07 PPR repeat
PPR_3 PF13812 208 - 263 3.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 219 - 247 5.1e-08 PPR repeat
PPR_2 PF13041 219 - 268 1.2e-15 PPR repeat family
PPR PF01535 222 - 252 8e-07 PPR repeat
PPR_1 PF12854 251 - 282 7.8e-08 PPR repeat
PPR_2 PF13041 255 - 300 7.4e-12 PPR repeat family
PPR PF01535 257 - 287 5.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 708
AccBSI CCGCTC 1 cut(s) 257
AccI GTMKAC 1 cut(s) 348
AciI CCGC 2 cut(s) 255, 372
AclWI GGATC 1 cut(s) 631
AcsI RAATTY 2 cut(s) 333, 393
AdeI CACNNNGTG 1 cut(s) 344
AfaI GTAC 3 cut(s) 129, 576, 805
AfeI AGCGCT 1 cut(s) 405
AfiI CCNNNNNNNGG 2 cut(s) 646, 708
AgsI TTSAA 6 cut(s) 22, 209, 398, 419, 944, 1036
AloI GAACNNNNNNTCC 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 306, 675, 719, 809, 1109
AluI AGCT 5 cut(s) 306, 675, 719, 809, 1109
Alw26I GTCTC 1 cut(s) 908
AlwI GGATC 1 cut(s) 631
AlwNI CAGNNNCTG 1 cut(s) 708
Aor51HI AGCGCT 1 cut(s) 405
AoxI GGCC 5 cut(s) 87, 233, 653, 822, 850
ApoI RAATTY 2 cut(s) 333, 393
AseI ATTAAT 1 cut(s) 684
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 2 cut(s) 406, 1063
AspS9I GGNCC 1 cut(s) 699
AsuC2I CCSGG 2 cut(s) 132, 854
AsuHPI GGTGA 4 cut(s) 238, 330, 1057, 1115
AvaII GGWCC 1 cut(s) 699
BbsI GAAGAC 1 cut(s) 608
BccI CCATC 2 cut(s) 5, 56
BceAI ACGGC 1 cut(s) 786
BcnI CCSGG 2 cut(s) 132, 854
BcoDI GTCTC 1 cut(s) 908
BfoI RGCGCY 1 cut(s) 407
Bme1390I CCNGG 2 cut(s) 132, 854
Bme18I GGWCC 1 cut(s) 699
BmgT120I GGNCC 1 cut(s) 699
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 132, 854
BmrI ACTGGG 2 cut(s) 336, 508
BmsI GCATC 3 cut(s) 571, 886, 1137
BmuI ACTGGG 2 cut(s) 336, 508
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 1 cut(s) 730
BpuMI CCSGG 2 cut(s) 132, 854
BsaJI CCNNGG 2 cut(s) 853, 1086
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 708
Bse1I ACTGG 4 cut(s) 342, 503, 582, 713
Bse3DI GCAATG 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 853, 1086
BseGI GGATG 4 cut(s) 586, 751, 901, 909
BseLI CCNNNNNNNGG 2 cut(s) 646, 708
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 973
BseNI ACTGG 4 cut(s) 342, 503, 582, 713
BseYI CCCAGC 2 cut(s) 183, 899
BshFI GGCC 5 cut(s) 89, 235, 655, 824, 852
BsiSI CCGG 2 cut(s) 131, 853
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 2 cut(s) 646, 708
BsmAI GTCTC 1 cut(s) 908
BsmFI GGGAC 1 cut(s) 870
BsmI GAATGC 1 cut(s) 744
BsnI GGCC 5 cut(s) 89, 235, 655, 824, 852
Bsp143I GATC 5 cut(s) 111, 220, 448, 636, 1153
BspACI CCGC 2 cut(s) 255, 372
BspANI GGCC 5 cut(s) 89, 235, 655, 824, 852
BspCNI CTCAG 1 cut(s) 972
BspLI GGNNCC 1 cut(s) 544
BspPI GGATC 1 cut(s) 631
BsrBI CCGCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 4 cut(s) 342, 503, 582, 713
BssECI CCNNGG 2 cut(s) 853, 1086
BssMI GATC 5 cut(s) 111, 220, 448, 636, 1153
BssNAI GTATAC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 1086
Bst1107I GTATAC 1 cut(s) 349
Bst4CI ACNGT 5 cut(s) 329, 361, 782, 874, 950
Bst6I CTCTTC 2 cut(s) 813, 906
BstDEI CTNAG 1 cut(s) 959
BstF5I GGATG 4 cut(s) 586, 751, 901, 909
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 2 cut(s) 406, 1063
BstKTI GATC 5 cut(s) 114, 223, 451, 639, 1156
BstMAI GTCTC 1 cut(s) 908
BstMBI GATC 5 cut(s) 111, 220, 448, 636, 1153
BstMWI GCNNNNNNNGC 2 cut(s) 378, 702
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 2 cut(s) 130, 852
BstV2I GAAGAC 1 cut(s) 608
BstZ17I GTATAC 1 cut(s) 349
BsuRI GGCC 5 cut(s) 89, 235, 655, 824, 852
BtsCI GGATG 4 cut(s) 586, 751, 901, 909
BtsIMutI CAGTG 4 cut(s) 334, 349, 614, 706
CaiI CAGNNNCTG 1 cut(s) 708
CfoI GCGC 2 cut(s) 406, 1063
Cfr13I GGNCC 1 cut(s) 699
Csp6I GTAC 3 cut(s) 128, 575, 804
CviAII CATG 5 cut(s) 161, 175, 483, 529, 796
CviQI GTAC 3 cut(s) 128, 575, 804
DdeI CTNAG 1 cut(s) 959
DpnI GATC 5 cut(s) 113, 222, 450, 638, 1155
DpnII GATC 5 cut(s) 111, 220, 448, 636, 1153
DraI TTTAAA 1 cut(s) 523
DraIII CACNNNGTG 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 813, 906
EarI CTCTTC 2 cut(s) 813, 906
Eco130I CCWWGG 1 cut(s) 1086
Eco147I AGGCCT 2 cut(s) 89, 655
Eco47I GGWCC 1 cut(s) 699
Eco47III AGCGCT 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 1086
EcoT22I ATGCAT 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 1086
FaeI CATG 5 cut(s) 164, 178, 486, 532, 799
FalI AAGNNNNNCTT 6 cut(s) 293, 325, 390, 422, 1016, 1048
FaqI GGGAC 1 cut(s) 870
FatI CATG 5 cut(s) 160, 174, 482, 528, 795
FblI GTMKAC 1 cut(s) 348
FokI GGATG 4 cut(s) 593, 758, 908, 916
GlaI GCGC 2 cut(s) 405, 1062
GsaI CCCAGC 2 cut(s) 187, 903
GsuI CTGGAG 1 cut(s) 730
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 5 cut(s) 89, 235, 655, 824, 852
HapII CCGG 2 cut(s) 131, 853
HhaI GCGC 2 cut(s) 406, 1063
Hin1II CATG 5 cut(s) 164, 178, 486, 532, 799
Hin6I GCGC 2 cut(s) 404, 1061
HinP1I GCGC 2 cut(s) 404, 1061
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 1 cut(s) 1107
HinfI GANTC 5 cut(s) 157, 228, 619, 858, 1098
HpaII CCGG 2 cut(s) 131, 853
HphI GGTGA 4 cut(s) 238, 330, 1057, 1115
Hpy166II GTNNAC 2 cut(s) 349, 1093
Hpy188III TCNNGA 5 cut(s) 310, 398, 1033, 1135, 1157
Hpy8I GTNNAC 2 cut(s) 349, 1093
HpyAV CCTTC 5 cut(s) 9, 500, 590, 743, 1018
HpyCH4III ACNGT 5 cut(s) 329, 361, 782, 874, 950
HpyCH4V TGCA 7 cut(s) 171, 269, 381, 486, 591, 975, 1003
HpyF10VI GCNNNNNNNGC 2 cut(s) 378, 702
HpyF3I CTNAG 1 cut(s) 959
Hsp92II CATG 5 cut(s) 164, 178, 486, 532, 799
HspAI GCGC 2 cut(s) 404, 1061
Kzo9I GATC 5 cut(s) 111, 220, 448, 636, 1153
LmnI GCTCC 2 cut(s) 542, 680
LweI GCATC 3 cut(s) 571, 886, 1137
MaeIII GTNAC 5 cut(s) 454, 494, 559, 664, 874
MalI GATC 5 cut(s) 113, 222, 450, 638, 1155
MbiI CCGCTC 1 cut(s) 257
MboI GATC 5 cut(s) 111, 220, 448, 636, 1153
MboII GAAGA 8 cut(s) 20, 200, 230, 398, 613, 641, 830, 923
MfeI CAATTG 1 cut(s) 939
MluCI AATT 9 cut(s) 178, 261, 333, 393, 463, 568, 681, 939, 1128
MlyI GAGTC 2 cut(s) 237, 852
MmeI TCCRAC 1 cut(s) 270
Mph1103I ATGCAT 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 8 cut(s) 462, 522, 680, 684, 732, 785, 1040, 1122
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 2 cut(s) 131, 853
MspR9I CCNGG 2 cut(s) 132, 854
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 744
MwoI GCNNNNNNNGC 2 cut(s) 378, 702
NciI CCSGG 2 cut(s) 132, 854
NdeII GATC 5 cut(s) 111, 220, 448, 636, 1153
NlaIII CATG 5 cut(s) 164, 178, 486, 532, 799
NlaIV GGNNCC 1 cut(s) 544
NmuCI GTSAC 2 cut(s) 454, 664
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 1 cut(s) 799
PacI TTAATTAA 1 cut(s) 684
PceI AGGCCT 2 cut(s) 89, 655
PctI GAATGC 1 cut(s) 744
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 157, 619, 1098
PflMI CCANNNNNTGG 1 cut(s) 708
PleI GAGTC 2 cut(s) 236, 852
PpsI GAGTC 2 cut(s) 236, 852
PshBI ATTAAT 1 cut(s) 684
PsiI TTATAA 1 cut(s) 147
PspFI CCCAGC 2 cut(s) 183, 899
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 1 cut(s) 699
PstNI CAGNNNCTG 1 cut(s) 708
RsaI GTAC 3 cut(s) 129, 576, 805
RsaNI GTAC 3 cut(s) 128, 575, 804
SaqAI TTAA 8 cut(s) 462, 522, 680, 684, 732, 785, 1040, 1122
Sau3AI GATC 5 cut(s) 111, 220, 448, 636, 1153
Sau96I GGNCC 1 cut(s) 699
SchI GAGTC 2 cut(s) 237, 852
ScrFI CCNGG 2 cut(s) 132, 854
SfaNI GCATC 3 cut(s) 571, 886, 1137
SinI GGWCC 1 cut(s) 699
Sse9I AATT 9 cut(s) 178, 261, 333, 393, 463, 568, 681, 939, 1128
SseBI AGGCCT 2 cut(s) 89, 655
SsiI CCGC 2 cut(s) 255, 372
SspI AATATT 3 cut(s) 556, 927, 995
StuI AGGCCT 2 cut(s) 89, 655
StyD4I CCNGG 2 cut(s) 130, 852
StyI CCWWGG 1 cut(s) 1086
TaaI ACNGT 5 cut(s) 329, 361, 782, 874, 950
TasI AATT 9 cut(s) 178, 261, 333, 393, 463, 568, 681, 939, 1128
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 3 cut(s) 157, 619, 1098
Tru1I TTAA 8 cut(s) 462, 522, 680, 684, 732, 785, 1040, 1122
Tru9I TTAA 8 cut(s) 462, 522, 680, 684, 732, 785, 1040, 1122
TscAI CASTG 4 cut(s) 334, 349, 621, 713
TseFI GTSAC 2 cut(s) 454, 664
Tsp45I GTSAC 2 cut(s) 454, 664
TspDTI ATGAA 5 cut(s) 149, 191, 471, 762, 968
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 4 cut(s) 334, 349, 621, 713
Van91I CCANNNNNTGG 1 cut(s) 708
VpaK11BI GGWCC 1 cut(s) 699
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 2 cut(s) 333, 393
XceI RCATGY 1 cut(s) 799
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 393
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.