pycom04g01200

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001472
Physical Location & Seq
Reverse (-)
1079605 .. 1080816
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g01200.1

Sequence Viewer

Length: 1212 bp
ATGAAGAGGATAACCACAAGACCTTCATGTCCAGGTAATCTTCCTTCCTTCCGTTTTACATTTCTGCAGAATACAAGTGGTGGATTTCATTCCGACGCTGTTCATGCTAATCCGACTTGTACGGATGGAATTAAACAATCCCATTTGGAGTCGGAGAGTATGATTCAAACTCGATGCAAATCTGGAAAAATTAGGAAAGATGAGGCATTGGGTTACTTAAATTCCATGATTCAAACAAGACACTTGCCCTCTATTTGGACTTTCAATTGTTTGCTTGGGGCACTCTCCAAGATGAAACAATATTCTACTGTCGTTTCCACGTGCAAACAGTTGATAGGTTGCGCTCAGTTCCAACGTGATGTCAGTACAATGACTATTTTTGCAAATTGCTTGTGCCGTCTGAATTGGGGGGACGCGTGTTTCTCTGTTCTAGCAATTACCGTTAAATATGGTCTTCAACCCGATGCTCATTTTATGGCTAGTTTGCTTCACGGGCTCTGCAAGAATAGTTCCCTGGCAGGGGCAATGGCGTTCTTCCAAGAAATAGAAACAAAAGGATACGCATGCGACGAAAGCACTTATGGTACCATGATCAATGGACTAGCCAAAGCTGGGAAAACTTCCAAGACACTTATCAGTTATACCACTTTGATTCATGGGTTAGGCAACATGAGTCAGTGGGATGAAGTGCTCTCTCTGTTTGAAAAAATGACGGATCAAGGAGTCGAGCCTAATGAAATCACTTATGCTACTATGATAAATGGATTATGCAAAGCTCGGAAAACTTCTAAGGTACTCGAGATACTAATAATGATGTGGAAAGATGGAAGGTTGAAGCCCGACCAAGGAAGCAACATTCCAATCATCAATAGCCTGTGTAAAAAAAGCCGAATAGATGAGGCCTTGACCCTATTTCGGGATATGACCAGCAAAAGTGTAGTACCAAATACTGTCACTTGTAACAGTTTAATTCATAGGTTATGCAGCATGAATCGCTGGGAAGAAGTGCTCTCTCTTTTTGAAGGAATGGTAGATCAAGGAGTCAAGCCTAATGTTGTCACCTACAACACTCTAATTTATGCATTGTGCCAATCGGGGAGGATGGAAAAGGCCAAGAGCTACTTGATTTGCGTGCTTAACAGTGGAATTTCACCAGATGTATACACGTATAATGTTCTTATCAGCAGTCTTTGCAAGGAAGAAAGAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

404

Amino Acids

44.84

Weight (kDa)

8.78

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 154 - 203 6e-09 PPR repeat family
PPR_2 PF13041 211 - 258 1.3e-15 PPR repeat family
PPR PF01535 212 - 242 3.4e-06 PPR repeat
TPR_24 PF23276 234 - 341 2.1e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 241 - 262 4.9e-07 PPR repeat
PPR_2 PF13041 288 - 328 6e-11 PPR repeat family
PPR_long PF17177 295 - 373 8.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 315 - 364 2.5e-17 PPR repeat family
PPR PF01535 318 - 348 7e-06 PPR repeat
PPR_3 PF13812 338 - 396 8.2e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 347 - 371 1.4e-10 PPR repeat
PPR_2 PF13041 350 - 399 1.4e-15 PPR repeat family
PPR PF01535 353 - 383 4.9e-06 PPR repeat
PPR_1 PF12854 382 - 402 4.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 27
Acc65I GGTACC 1 cut(s) 584
AccB1I GGYRCC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 1161
AccII CGCG 1 cut(s) 416
AclWI GGATC 1 cut(s) 723
AcsI RAATTY 3 cut(s) 220, 1146, 1206
AcvI CACGTG 1 cut(s) 321
AfaI GTAC 5 cut(s) 121, 367, 586, 795, 942
AfiI CCNNNNNNNGG 7 cut(s) 255, 519, 520, 612, 845, 915, 916
AflIII ACRYGT 2 cut(s) 414, 1164
AgsI TTSAA 7 cut(s) 167, 233, 265, 458, 704, 835, 1022
AjnI CCWGG 2 cut(s) 31, 513
AluBI AGCT 3 cut(s) 611, 776, 1119
AluI AGCT 3 cut(s) 611, 776, 1119
Alw21I GWGCWC 2 cut(s) 693, 1011
AlwI GGATC 1 cut(s) 723
Ama87I CYCGRG 1 cut(s) 797
AoxI GGCC 2 cut(s) 900, 1110
ApeKI GCWGC 1 cut(s) 984
ApoI RAATTY 3 cut(s) 220, 1146, 1206
Asp718I GGTACC 1 cut(s) 584
AspLEI GCGC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 1051, 1143
AvaI CYCGRG 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 283
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 498
BarI GAAGNNNNNNTAC 2 cut(s) 28, 60
BbrPI CACGTG 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 693, 1011
BbvI GCAGC 1 cut(s) 996
BccI CCATC 3 cut(s) 119, 818, 1096
BceAI ACGGC 1 cut(s) 381
BciT130I CCWGG 2 cut(s) 33, 515
BciVI GTATCC 1 cut(s) 551
BclI TGATCA 1 cut(s) 591
BfaI CTAG 3 cut(s) 431, 480, 602
BfmI CTRYAG 1 cut(s) 65
BfuI GTATCC 1 cut(s) 551
BisI GCNGC 1 cut(s) 985
BlsI GCNGC 1 cut(s) 986
Bme1390I CCNGG 2 cut(s) 33, 515
BmeT110I CYCGRG 1 cut(s) 797
BmiI GGNNCC 1 cut(s) 586
BmrFI CCNGG 2 cut(s) 33, 515
BmsI GCATC 2 cut(s) 164, 454
BpiI GAAGAC 1 cut(s) 446
BsaAI YACGTR 2 cut(s) 321, 1167
BsaBI GATNNNNATC 1 cut(s) 178
BsaJI CCNNGG 2 cut(s) 513, 844
Bsc4I CCNNNNNNNGG 7 cut(s) 255, 519, 520, 612, 845, 915, 916
Bse3DI GCAATG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 178
BseBI CCWGG 2 cut(s) 33, 515
BseDI CCNNGG 2 cut(s) 513, 844
BseGI GGATG 3 cut(s) 130, 688, 1107
BseJI GATNNNNATC 1 cut(s) 178
BseLI CCNNNNNNNGG 7 cut(s) 255, 519, 520, 612, 845, 915, 916
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 1 cut(s) 996
BseYI CCCAGC 2 cut(s) 611, 996
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 902, 1112
BshNI GGYRCC 1 cut(s) 584
BsiHKAI GWGCWC 2 cut(s) 693, 1011
BsiHKCI CYCGRG 1 cut(s) 797
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 7 cut(s) 255, 519, 520, 612, 845, 915, 916
BsmFI GGGAC 1 cut(s) 425
BsnI GGCC 2 cut(s) 902, 1112
BsoBI CYCGRG 1 cut(s) 797
Bsp1286I GDGCHC 4 cut(s) 283, 498, 693, 1011
Bsp143I GATC 3 cut(s) 591, 715, 1033
BspANI GGCC 2 cut(s) 902, 1112
BspCNI CTCAG 1 cut(s) 358
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 1 cut(s) 586
BspMAI CTGCAG 1 cut(s) 69
BspPI GGATC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 584
BsrDI GCAATG 1 cut(s) 531
BssECI CCNNGG 2 cut(s) 513, 844
BssMI GATC 3 cut(s) 591, 715, 1033
BssNAI GTATAC 1 cut(s) 1162
BssT1I CCWWGG 1 cut(s) 844
Bst1107I GTATAC 1 cut(s) 1162
Bst2UI CCWGG 2 cut(s) 33, 515
Bst4CI ACNGT 6 cut(s) 310, 330, 442, 952, 965, 1142
BstAPI GCANNNNNTGC 1 cut(s) 1191
BstBAI YACGTR 2 cut(s) 321, 1167
BstC8I GCNNGC 2 cut(s) 565, 1133
BstDEI CTNAG 2 cut(s) 345, 789
BstF5I GGATG 3 cut(s) 130, 688, 1107
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 3 cut(s) 594, 718, 1036
BstMBI GATC 3 cut(s) 591, 715, 1033
BstMWI GCNNNNNNNGC 5 cut(s) 104, 493, 573, 993, 1191
BstNI CCWGG 2 cut(s) 33, 515
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 2 cut(s) 31, 513
BstSFI CTRYAG 1 cut(s) 65
BstSLI GKGCMC 1 cut(s) 283
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 1 cut(s) 996
BstV2I GAAGAC 1 cut(s) 446
BstZ17I GTATAC 1 cut(s) 1162
BsuI GTATCC 1 cut(s) 551
BsuRI GGCC 2 cut(s) 902, 1112
BtsCI GGATG 3 cut(s) 130, 688, 1107
BtsIMutI CAGTG 2 cut(s) 683, 1147
Cac8I GCNNGC 2 cut(s) 565, 1133
CfoI GCGC 1 cut(s) 344
CseI GACGC 2 cut(s) 104, 422
Csp6I GTAC 5 cut(s) 120, 366, 585, 794, 941
CviAII CATG 8 cut(s) 27, 104, 226, 564, 589, 656, 670, 988
CviQI GTAC 5 cut(s) 120, 366, 585, 794, 941
DdeI CTNAG 2 cut(s) 345, 789
DpnI GATC 3 cut(s) 593, 717, 1035
DpnII GATC 3 cut(s) 591, 715, 1033
DrdI GACNNNNNNGTC 1 cut(s) 27
DseDI GACNNNNNNGTC 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 844
Eco147I AGGCCT 1 cut(s) 902
Eco24I GRGCYC 1 cut(s) 498
Eco72I CACGTG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 797
EcoRII CCWGG 2 cut(s) 31, 513
EcoT14I CCWWGG 1 cut(s) 844
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 844
FaeI CATG 8 cut(s) 30, 107, 229, 567, 592, 659, 673, 991
FalI AAGNNNNNCTT 2 cut(s) 1106, 1138
FaqI GGGAC 1 cut(s) 425
FatI CATG 8 cut(s) 26, 103, 225, 563, 588, 655, 669, 987
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 1 cut(s) 1161
Fnu4HI GCNGC 1 cut(s) 985
FokI GGATG 3 cut(s) 137, 695, 1114
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 1 cut(s) 985
FspBI CTAG 3 cut(s) 431, 480, 602
GlaI GCGC 1 cut(s) 343
GluI GCNGC 1 cut(s) 985
GsaI CCCAGC 2 cut(s) 615, 1000
HaeIII GGCC 2 cut(s) 902, 1112
HgaI GACGC 2 cut(s) 104, 422
HhaI GCGC 1 cut(s) 344
Hin1II CATG 8 cut(s) 30, 107, 229, 567, 592, 659, 673, 991
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 8 cut(s) 149, 163, 229, 652, 673, 723, 991, 1041
HphI GGTGA 2 cut(s) 1051, 1143
Hpy166II GTNNAC 1 cut(s) 1162
Hpy188I TCNGA 5 cut(s) 94, 114, 154, 402, 780
Hpy188III TCNNGA 3 cut(s) 183, 799, 917
Hpy8I GTNNAC 1 cut(s) 1162
Hpy99I CGWCG 2 cut(s) 98, 572
HpyAV CCTTC 5 cut(s) 33, 54, 58, 822, 1016
HpyCH4III ACNGT 6 cut(s) 310, 330, 442, 952, 965, 1142
HpyCH4IV ACGT 3 cut(s) 320, 355, 1166
HpyCH4V TGCA 9 cut(s) 67, 177, 324, 383, 501, 771, 984, 1082, 1194
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 493, 573, 993, 1191
HpyF3I CTNAG 2 cut(s) 345, 789
HpySE526I ACGT 3 cut(s) 320, 355, 1166
Hsp92II CATG 8 cut(s) 30, 107, 229, 567, 592, 659, 673, 991
HspAI GCGC 1 cut(s) 342
KpnI GGTACC 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 3 cut(s) 591, 715, 1033
Lsp1109I GCAGC 1 cut(s) 996
LweI GCATC 2 cut(s) 164, 454
MaeI CTAG 3 cut(s) 431, 480, 602
MaeII ACGT 3 cut(s) 320, 355, 1166
MaeIII GTNAC 4 cut(s) 212, 952, 959, 1057
MalI GATC 3 cut(s) 593, 717, 1035
MboI GATC 3 cut(s) 591, 715, 1033
MboII GAAGA 6 cut(s) 16, 32, 446, 526, 1013, 1211
MfeI CAATTG 1 cut(s) 265
MhlI GDGCHC 4 cut(s) 283, 498, 693, 1011
MluI ACGCGT 1 cut(s) 414
MlyI GAGTC 4 cut(s) 158, 682, 732, 1050
MmeI TCCRAC 4 cut(s) 117, 132, 137, 376
MnlI CCTC 4 cut(s) 196, 259, 892, 1092
Mph1103I ATGCAT 1 cut(s) 1084
MseI TTAA 6 cut(s) 132, 218, 444, 968, 1137, 1210
MspR9I CCNGG 2 cut(s) 33, 515
MunI CAATTG 1 cut(s) 265
MvaI CCWGG 2 cut(s) 33, 515
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 5 cut(s) 104, 493, 573, 993, 1191
NdeII GATC 3 cut(s) 591, 715, 1033
NlaIII CATG 8 cut(s) 30, 107, 229, 567, 592, 659, 673, 991
NlaIV GGNNCC 1 cut(s) 586
NmuCI GTSAC 2 cut(s) 952, 1057
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 1 cut(s) 567
PaeI GCATGC 1 cut(s) 567
PaeR7I CTCGAG 1 cut(s) 797
PceI AGGCCT 1 cut(s) 902
PcsI WCGNNNNNNNCGW 1 cut(s) 567
PfeI GAWTC 4 cut(s) 163, 229, 652, 991
PkrI GCNGC 1 cut(s) 986
PleI GAGTC 4 cut(s) 157, 681, 731, 1049
PmaCI CACGTG 1 cut(s) 321
PmlI CACGTG 1 cut(s) 321
PpsI GAGTC 4 cut(s) 157, 681, 731, 1049
Ppu21I YACGTR 2 cut(s) 321, 1167
Psp6I CCWGG 2 cut(s) 31, 513
PspCI CACGTG 1 cut(s) 321
PspFI CCCAGC 2 cut(s) 611, 996
PspGI CCWGG 2 cut(s) 31, 513
PspN4I GGNNCC 1 cut(s) 586
PstI CTGCAG 1 cut(s) 69
RsaI GTAC 5 cut(s) 121, 367, 586, 795, 942
RsaNI GTAC 5 cut(s) 120, 366, 585, 794, 941
SaqAI TTAA 6 cut(s) 132, 218, 444, 968, 1137, 1210
SatI GCNGC 1 cut(s) 985
Sau3AI GATC 3 cut(s) 591, 715, 1033
SchI GAGTC 4 cut(s) 158, 682, 732, 1050
ScrFI CCNGG 2 cut(s) 33, 515
SduI GDGCHC 4 cut(s) 283, 498, 693, 1011
SfaNI GCATC 2 cut(s) 164, 454
SfcI CTRYAG 1 cut(s) 65
Sfr274I CTCGAG 1 cut(s) 797
SlaI CTCGAG 1 cut(s) 797
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
SphI GCATGC 1 cut(s) 567
SseBI AGGCCT 1 cut(s) 902
SspI AATATT 1 cut(s) 302
SspMI CTAG 3 cut(s) 431, 480, 602
StuI AGGCCT 1 cut(s) 902
StyD4I CCNGG 2 cut(s) 31, 513
StyI CCWWGG 1 cut(s) 844
TaaI ACNGT 6 cut(s) 310, 330, 442, 952, 965, 1142
TaiI ACGT 3 cut(s) 323, 358, 1169
TaqI TCGA 3 cut(s) 172, 726, 798
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 4 cut(s) 163, 229, 652, 991
Tru1I TTAA 6 cut(s) 132, 218, 444, 968, 1137, 1210
Tru9I TTAA 6 cut(s) 132, 218, 444, 968, 1137, 1210
TscAI CASTG 2 cut(s) 683, 1147
TseFI GTSAC 2 cut(s) 952, 1057
TseI GCWGC 1 cut(s) 984
Tsp45I GTSAC 2 cut(s) 952, 1057
TspGWI ACGGA 3 cut(s) 41, 137, 728
TspRI CASTG 2 cut(s) 683, 1147
XapI RAATTY 3 cut(s) 220, 1146, 1206
XceI RCATGY 1 cut(s) 567
XhoI CTCGAG 1 cut(s) 797
XmiI GTMKAC 1 cut(s) 1161
XspI CTAG 3 cut(s) 431, 480, 602
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.