pycom04g10990

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001472
Physical Location & Seq
Forward (+)
13817939 .. 13819583
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10990.2

Sequence Viewer

Length: 1353 bp
ATGAAGATAAAGGTGATAACCACGAGGCTTCCTAACTCAACAGATAGATTTAATCAAACCCATTCGGAATCGGACAATTTGATTCAAACTCGATGCAAATCTGGAAAGATTAGGAAAGATGAAGCATTCGGTTACTTCGATTCCATGATTCAAACCAAACCCTTGCCCTCTATTTGGACTTTCAATTGTTTGTTTGGGGCACTCTCCAAGATGAAGGAGTATTCTGCTGTGGTTTCTATGTGTAAACAGTTGATGGGTTGCGCTCAATTCCGACCTGATGTCTGTACAATGAATATTGTTCTGAATTGCTTGTGCCGTCTGAACCGGGTGGACTTGGGTTTCTCTGTTTTAGCAACTACCCTTAAATATGGTCTTCAACCCGATGCTCATTCTGTAAGTATTTTACTTCACGGACTTTGCAAGTACAGTTCCTGGGACGAGGCAATGGAGCTGTTCAAGTTAATAGAAGAGAAGGGATACACATGCAATGAAATCACTTATGCTACCATGATCAATGGACTATGCAAGGCTGGGAAAACTTCTAAGGCACTCGAGATACTTATGAATATGTTGGAAGATGGAAGGTTCAAGCCCAAACAAGACTGCTACAATCCCATCATAGATAGATTGTGTAAGGAAAGACAAGTAGATGAGGCCTTGACCCTATTTCGAGATATGATCAGCAAAAGTGTTGTACCGGATATTATCACTTATAACACTTTGATTCATGGGTTATGCAACATGAGTCGCTGGGAAGAAGTGCTCTCTCTTTTTGAAGCAATGGATGATCAAGGAGTCAGGCCAGATGTTTTAACCTACACCGCTTTAATTTACGCATTGTGCCAATTGGGGAGGTTGGAAAAAGCCAAGAGCTTCTTGATTTGCATGCTTAACAGTGGGATTTCACCCAGTGTATACACATATAATGTTCTTATCAGCGGTCTTTGCAAGGAAGAAAGAATTCAAGAAGCACTTACTCTGTTTGAAAAGATGACAATGAGAGGCATAAAGCCTGATGTTGTCACTTTTAATTGCTTGATTTCTGCTTCATGCAAGTCTGGTAACTGGGAGGAAGTTATGAGTTTATTTAAAACCATGATTGGTTATGGAGCCTTGCCTAATATTTTTACATATAATTCTGTACTGGATGCTTTATGCAAGGAAGGGAAGACAGCAGAGGCACTGAATCTTGTGGAAGAAATGATCCGTAGAGAGGAAAAGCCTAATGTTGTGACTTACAACTCCTTAATTAATGGATTGTGCCAGACCAGCCAATGGAGAGAAGCTACAAGGGTGTTTAATAGAATGCTGGATGACGGTATTGCCCCAGATGTTATCACTTACAACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

451

Amino Acids

51.01

Weight (kDa)

6.87

Isoelectric Point (pI)

30.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AccB7I CCANNNNNTGG 1 cut(s) 1275
AccI GTMKAC 1 cut(s) 915
AciI CCGC 2 cut(s) 822, 939
AclWI GGATC 1 cut(s) 1198
AcsI RAATTY 1 cut(s) 960
AdeI CACNNNGTG 1 cut(s) 911
AfaI GTAC 4 cut(s) 286, 425, 696, 1143
AfiI CCNNNNNNNGG 3 cut(s) 174, 1213, 1275
AgsI TTSAA 9 cut(s) 86, 152, 184, 377, 457, 589, 776, 965, 986
AhdI GACNNNNNGTC 1 cut(s) 278
AjnI CCWGG 1 cut(s) 431
AluBI AGCT 3 cut(s) 451, 873, 1286
AluI AGCT 3 cut(s) 451, 873, 1286
Alw21I GWGCWC 1 cut(s) 765
AlwI GGATC 1 cut(s) 1198
AlwNI CAGNNNCTG 1 cut(s) 432
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 2 cut(s) 654, 800
ApoI RAATTY 1 cut(s) 960
AseI ATTAAT 1 cut(s) 1251
Asp700I GAANNNNTTC 1 cut(s) 960
AspLEI GCGC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 25, 897
AvaI CYCGRG 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 202
BauI CACGAG 1 cut(s) 22
BbsI GAAGAC 2 cut(s) 365, 1175
Bbv12I GWGCWC 1 cut(s) 765
BccI CCATC 3 cut(s) 247, 572, 623
BceAI ACGGC 1 cut(s) 300
BciT130I CCWGG 1 cut(s) 433
BciVI GTATCC 1 cut(s) 470
BclI TGATCA 3 cut(s) 510, 678, 787
BcnI CCSGG 1 cut(s) 326
BfuI GTATCC 1 cut(s) 470
Bme1390I CCNGG 2 cut(s) 326, 433
BmeRI GACNNNNNGTC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 551
BmiI GGNNCC 1 cut(s) 1111
BmrFI CCNGG 2 cut(s) 326, 433
BmrI ACTGGG 2 cut(s) 903, 1075
BmsI GCATC 3 cut(s) 83, 373, 1138
BmuI ACTGGG 2 cut(s) 903, 1075
BpiI GAAGAC 2 cut(s) 365, 1175
BpuMI CCSGG 1 cut(s) 326
BsaBI GATNNNNATC 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 432
BsaWI WCCGGW 1 cut(s) 697
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 1213, 1275
Bse1I ACTGG 3 cut(s) 909, 1070, 1149
Bse3DI GCAATG 3 cut(s) 450, 493, 786
Bse8I GATNNNNATC 1 cut(s) 97
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 1 cut(s) 432
BseGI GGATG 3 cut(s) 790, 1153, 1318
BseJI GATNNNNATC 1 cut(s) 97
BseLI CCNNNNNNNGG 3 cut(s) 174, 1213, 1275
BseMI GCAATG 3 cut(s) 450, 493, 786
BseNI ACTGG 3 cut(s) 909, 1070, 1149
BseSI GKGCMC 1 cut(s) 202
BseYI CCCAGC 2 cut(s) 530, 750
BshFI GGCC 2 cut(s) 656, 802
BsiHKAI GWGCWC 1 cut(s) 765
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 2 cut(s) 325, 698
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 3 cut(s) 174, 1213, 1275
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 2 cut(s) 125, 1311
BsnI GGCC 2 cut(s) 656, 802
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 2 cut(s) 202, 765
Bsp1407I TGTACA 1 cut(s) 284
Bsp143I GATC 4 cut(s) 510, 678, 787, 1203
BspACI CCGC 2 cut(s) 822, 939
BspANI GGCC 2 cut(s) 656, 802
BspLI GGNNCC 1 cut(s) 1111
BspPI GGATC 1 cut(s) 1198
BsrDI GCAATG 3 cut(s) 450, 493, 786
BsrGI TGTACA 1 cut(s) 284
BsrI ACTGG 3 cut(s) 909, 1070, 1149
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 4 cut(s) 510, 678, 787, 1203
BssNAI GTATAC 1 cut(s) 916
BssSI CACGAG 1 cut(s) 22
Bst1107I GTATAC 1 cut(s) 916
Bst2BI CACGAG 1 cut(s) 22
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 4 cut(s) 249, 428, 896, 1319
Bst6I CTCTTC 1 cut(s) 462
BstAUI TGTACA 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 887
BstDEI CTNAG 1 cut(s) 543
BstF5I GGATG 3 cut(s) 790, 1153, 1318
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 4 cut(s) 513, 681, 790, 1206
BstMBI GATC 4 cut(s) 510, 678, 787, 1203
BstMWI GCNNNNNNNGC 2 cut(s) 945, 1269
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 2 cut(s) 486, 889
BstSCI CCNGG 2 cut(s) 324, 431
BstSLI GKGCMC 1 cut(s) 202
BstV2I GAAGAC 2 cut(s) 365, 1175
BstZ17I GTATAC 1 cut(s) 916
BsuI GTATCC 1 cut(s) 470
BsuRI GGCC 2 cut(s) 656, 802
BtsCI GGATG 3 cut(s) 790, 1153, 1318
BtsIMutI CAGTG 3 cut(s) 901, 916, 1181
Cac8I GCNNGC 1 cut(s) 887
CaiI CAGNNNCTG 1 cut(s) 432
CfoI GCGC 1 cut(s) 263
Csp6I GTAC 4 cut(s) 285, 424, 695, 1142
CviAII CATG 8 cut(s) 145, 483, 508, 728, 742, 886, 1050, 1096
CviQI GTAC 4 cut(s) 285, 424, 695, 1142
DdeI CTNAG 1 cut(s) 543
DpnI GATC 4 cut(s) 512, 680, 789, 1205
DpnII GATC 4 cut(s) 510, 678, 787, 1203
DraI TTTAAA 1 cut(s) 1090
DraIII CACNNNGTG 1 cut(s) 911
DriI GACNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 1 cut(s) 462
Eam1105I GACNNNNNGTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 960
EcoRII CCWGG 1 cut(s) 431
FaeI CATG 8 cut(s) 148, 486, 511, 731, 745, 889, 1053, 1099
FalI AAGNNNNNCTT 4 cut(s) 860, 892, 957, 989
FaqI GGGAC 1 cut(s) 449
FatI CATG 8 cut(s) 144, 482, 507, 727, 741, 885, 1049, 1095
FbaI TGATCA 3 cut(s) 510, 678, 787
FblI GTMKAC 1 cut(s) 915
FokI GGATG 3 cut(s) 797, 1160, 1325
GlaI GCGC 1 cut(s) 262
GsaI CCCAGC 2 cut(s) 534, 754
HaeIII GGCC 2 cut(s) 656, 802
HapII CCGG 2 cut(s) 325, 698
HhaI GCGC 1 cut(s) 263
Hin1II CATG 8 cut(s) 148, 486, 511, 731, 745, 889, 1053, 1099
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HinfI GANTC 8 cut(s) 68, 82, 140, 148, 724, 745, 795, 1186
HpaII CCGG 2 cut(s) 325, 698
HphI GGTGA 2 cut(s) 25, 897
Hpy166II GTNNAC 3 cut(s) 245, 331, 916
Hpy188I TCNGA 5 cut(s) 67, 73, 272, 303, 321
Hpy188III TCNNGA 5 cut(s) 102, 553, 671, 877, 965
Hpy8I GTNNAC 3 cut(s) 245, 331, 916
HpyAV CCTTC 4 cut(s) 208, 466, 576, 1157
HpyCH4III ACNGT 4 cut(s) 249, 428, 896, 1319
HpyCH4V TGCA 9 cut(s) 96, 420, 486, 525, 738, 885, 948, 1053, 1158
HpyF10VI GCNNNNNNNGC 2 cut(s) 945, 1269
HpyF3I CTNAG 1 cut(s) 543
Hsp92II CATG 8 cut(s) 148, 486, 511, 731, 745, 889, 1053, 1099
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 3 cut(s) 510, 678, 787
Kzo9I GATC 4 cut(s) 510, 678, 787, 1203
LmnI GCTCC 2 cut(s) 448, 1109
LweI GCATC 3 cut(s) 83, 373, 1138
MaeIII GTNAC 4 cut(s) 131, 1021, 1061, 1231
MalI GATC 4 cut(s) 512, 680, 789, 1205
MboI GATC 4 cut(s) 510, 678, 787, 1203
MboII GAAGA 8 cut(s) 16, 365, 479, 587, 767, 965, 1180, 1208
MfeI CAATTG 2 cut(s) 184, 845
MhlI GDGCHC 2 cut(s) 202, 765
MlyI GAGTC 2 cut(s) 754, 804
MmeI TCCRAC 3 cut(s) 295, 552, 837
MnlI CCTC 9 cut(s) 18, 178, 433, 646, 846, 995, 1063, 1171, 1207
MroXI GAANNNNTTC 1 cut(s) 960
MspA1I CMGCKG 1 cut(s) 939
MspI CCGG 2 cut(s) 325, 698
MspR9I CCNGG 2 cut(s) 326, 433
MunI CAATTG 2 cut(s) 184, 845
Mva1269I GAATGC 2 cut(s) 125, 1311
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 2 cut(s) 945, 1269
NciI CCSGG 1 cut(s) 326
NdeII GATC 4 cut(s) 510, 678, 787, 1203
NlaIII CATG 8 cut(s) 148, 486, 511, 731, 745, 889, 1053, 1099
NlaIV GGNNCC 1 cut(s) 1111
NmuCI GTSAC 2 cut(s) 1021, 1231
NspI RCATGY 2 cut(s) 486, 889
PacI TTAATTAA 1 cut(s) 1251
PaeI GCATGC 1 cut(s) 889
PaeR7I CTCGAG 1 cut(s) 551
PceI AGGCCT 1 cut(s) 656
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 2 cut(s) 125, 1311
PdmI GAANNNNTTC 1 cut(s) 960
PfeI GAWTC 6 cut(s) 68, 82, 140, 148, 724, 1186
PflMI CCANNNNNTGG 1 cut(s) 1275
PleI GAGTC 2 cut(s) 753, 803
PpsI GAGTC 2 cut(s) 753, 803
PshBI ATTAAT 1 cut(s) 1251
PsiI TTATAA 1 cut(s) 714
Psp6I CCWGG 1 cut(s) 431
PspFI CCCAGC 2 cut(s) 530, 750
PspGI CCWGG 1 cut(s) 431
PspN4I GGNNCC 1 cut(s) 1111
PstNI CAGNNNCTG 1 cut(s) 432
RsaI GTAC 4 cut(s) 286, 425, 696, 1143
RsaNI GTAC 4 cut(s) 285, 424, 695, 1142
Sau3AI GATC 4 cut(s) 510, 678, 787, 1203
SchI GAGTC 2 cut(s) 754, 804
ScrFI CCNGG 2 cut(s) 326, 433
SduI GDGCHC 2 cut(s) 202, 765
SetI ASST 8 cut(s) 15, 277, 453, 587, 818, 857, 875, 1288
SfaNI GCATC 3 cut(s) 83, 373, 1138
Sfr274I CTCGAG 1 cut(s) 551
SlaI CTCGAG 1 cut(s) 551
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
SphI GCATGC 1 cut(s) 889
SseBI AGGCCT 1 cut(s) 656
SsiI CCGC 2 cut(s) 822, 939
SspI AATATT 2 cut(s) 295, 1123
StuI AGGCCT 1 cut(s) 656
StyD4I CCNGG 2 cut(s) 324, 431
TaaI ACNGT 4 cut(s) 249, 428, 896, 1319
TaqI TCGA 4 cut(s) 91, 138, 552, 670
TatI WGTACW 3 cut(s) 284, 423, 1141
TfiI GAWTC 6 cut(s) 68, 82, 140, 148, 724, 1186
TscAI CASTG 3 cut(s) 901, 916, 1188
TseFI GTSAC 2 cut(s) 1021, 1231
Tsp45I GTSAC 2 cut(s) 1021, 1231
TspDTI ATGAA 8 cut(s) 17, 135, 227, 305, 504, 578, 716, 1038
TspGWI ACGGA 2 cut(s) 426, 1196
TspRI CASTG 3 cut(s) 901, 916, 1188
Van91I CCANNNNNTGG 1 cut(s) 1275
VspI ATTAAT 1 cut(s) 1251
XapI RAATTY 1 cut(s) 960
XceI RCATGY 2 cut(s) 486, 889
XhoI CTCGAG 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.