MD04G1014400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001472
Physical Location & Seq
Reverse (-)
1613084 .. 1614446
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1014400.v1.1.491

Sequence Viewer

Length: 1308 bp
ATGATCAATGGGTTATGCAAGGCTGGGAAAACTTCTAAGGCACTAGAGATACTAATGAAGATGTGGGAAGATGGAAGGTTCAAGCCCAACCAAGAATGCTACAATCCAATCATTGATAGCCTCTGTAAAGAAAGACGAATAGACGAGGCCTTGACCCTATTTCGAGATATGATCAGCAAAAGTGTTGTACCGAATGTTTTCAATTATAACACATTGATTCATGGGTTATGCAACACGAGTCGCTGGGAAGAAGTGCTCTCTCTTTTGGAAGCAATGGAAGATCAAGGAGTCAAGCCAGATGTTTTAACCTACACCGCTCTAATTTATGCATTGTGCCAATTGGGGAGGTTGGAAAAAGCCAAGAGCTTCTTGATTTGCATGCTTAACAGTGGAATTTCACCCAATGTATACACATATAATGTTCTTATCAGCGGTCTTTGCAAGGAAGAAAGAATTCAAGAAGCGCTTGCTCTATTTGAAAAGATGACAATGAGAGGCATAAAGCCTGATGTTGTCACTTTTAATTGCTTGATTTCTACTTCATGCAAGTCTGGTAACTGGGAGGAAGTTATGAGTTTATTTAAAACCATGATTGGTTATGGAGCCTTGCCTGATATTGTTACATATAATTCTGTACTGGATGCTTTATGCAAGGAAGGGAAGACAGCAGAGGCACTGAATCTTGTGGAAGAAATGATCCATAGAGGGGAAAAGCCTGATGTTGTGACTTACAACTCCTTAATTAATGGATTGTGCCGTACAGCTCAATGGGAAGAAGCTACAAGGGTGTTTAATAGAATGCTGGATGAAGGTGTTGCCCCAGATGTTATCACTTACAACAGTTTAATCAATGGCATGTGCCGTACAGCTCAATGGGAAGAAGCCACAAGGTTGTTTGATGATATGGTAGGCCGGGGACTCTTGCCTGATACCGTTACTTTGGTAATACTTTTGGATGCTATCTACAAAGGTGGGATGACAGAAGAGACTCACAAAATATTTGAGGCAACAATTGAATACGGTATGAAACTCAGCACAATAACTTGCAGTATGCTGGTTAGTGAACATTGTTTGCAGGGCAGAATGGATAAGGCGAAGGTGGTGTTGAACTTAATGGTGATTATGGGTTATGCGCCTGATATTGCTTCCTATAAAACCTTGGTCAACGGCTATATAAACGTTAACAGAATCGGTGAAGCTTTGAGGCTTGTTAAAGAAATGATCCAAATAGGATTGATGCCTGATCTGGAAATGCAACGAACTTTGGGGCGTTTTCGTCGTAAAAGCCATGTTGGATTAGCAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

436

Amino Acids

48.94

Weight (kDa)

5.76

Isoelectric Point (pI)

26.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 27 - 57 8.6e-09 PPR repeat
PPR_2 PF13041 29 - 78 6.1e-15 PPR repeat family
PPR PF01535 33 - 61 5.7e-06 PPR repeat
PPR_long PF17177 35 - 190 1.5e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 61 - 93 2.3e-06 PPR repeat
PPR_2 PF13041 64 - 113 1.8e-16 PPR repeat family
PPR PF01535 69 - 97 1e-05 PPR repeat
PPR_3 PF13812 87 - 144 1.6e-12 Pentatricopeptide repeat domain
TPR_24 PF23276 89 - 196 2.7e-10 Fungal tetratrico peptide repeats
PPR_1 PF12854 96 - 127 1.2e-08 PPR repeat
PPR_2 PF13041 101 - 148 6.6e-15 PPR repeat family
PPR PF01535 103 - 132 5.4e-06 PPR repeat
PPR_1 PF12854 131 - 163 2.4e-13 PPR repeat
PPR_long PF17177 137 - 232 2.2e-06 Pentacotripeptide-repeat region of PRORP
PPR PF01535 137 - 167 1.1e-08 PPR repeat
PPR_2 PF13041 138 - 183 5.4e-16 PPR repeat family
PPR_3 PF13812 158 - 216 6.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 165 - 197 3.9e-09 PPR repeat
PPR_2 PF13041 169 - 210 5.5e-11 PPR repeat family
PPR PF01535 172 - 201 4.1e-06 PPR repeat
PPR_1 PF12854 201 - 232 9.6e-11 PPR repeat
PPR_2 PF13041 204 - 253 5.6e-20 PPR repeat family
PPR PF01535 207 - 236 1.3e-07 PPR repeat
PPR_long PF17177 224 - 348 1e-05 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 228 - 284 1.8e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 236 - 267 1e-11 PPR repeat
PPR_2 PF13041 239 - 288 9.3e-21 PPR repeat family
PPR PF01535 242 - 272 1.8e-08 PPR repeat
PPR_1 PF12854 271 - 303 7.1e-12 PPR repeat
PPR_2 PF13041 274 - 321 1.3e-15 PPR repeat family
PPR PF01535 277 - 307 1.1e-07 PPR repeat
PPR_2 PF13041 345 - 392 1.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AccBSI CCGCTC 1 cut(s) 317
AccI GTMKAC 1 cut(s) 408
AciI CCGC 2 cut(s) 315, 432
AclI AACGTT 1 cut(s) 1179
AclWI GGATC 2 cut(s) 691, 1216
AcsI RAATTY 2 cut(s) 393, 453
AfaI GTAC 4 cut(s) 189, 636, 760, 865
AfeI AGCGCT 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 706
AgsI TTSAA 6 cut(s) 82, 202, 458, 479, 1016, 1108
AluBI AGCT 5 cut(s) 366, 764, 779, 869, 1199
AluI AGCT 5 cut(s) 366, 764, 779, 869, 1199
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 980
AlwI GGATC 2 cut(s) 691, 1216
Aor51HI AGCGCT 1 cut(s) 465
AoxI GGCC 2 cut(s) 147, 910
ApoI RAATTY 2 cut(s) 393, 453
AseI ATTAAT 1 cut(s) 744
Asp700I GAANNNNTTC 2 cut(s) 197, 453
AspLEI GCGC 2 cut(s) 466, 1135
AsuC2I CCSGG 1 cut(s) 914
AsuHPI GGTGA 3 cut(s) 390, 1129, 1205
BauI CACGAG 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 258
BccI CCATC 1 cut(s) 65
BceAI ACGGC 3 cut(s) 741, 846, 1183
BclI TGATCA 2 cut(s) 3, 171
BcnI CCSGG 1 cut(s) 914
BcoDI GTCTC 1 cut(s) 980
BfaI CTAG 1 cut(s) 44
BfoI RGCGCY 1 cut(s) 467
Bme1390I CCNGG 1 cut(s) 914
BmiI GGNNCC 1 cut(s) 604
BmrFI CCNGG 1 cut(s) 914
BmrI ACTGGG 1 cut(s) 568
BmsI GCATC 3 cut(s) 631, 946, 1227
BmuI ACTGGG 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 914
BsaJI CCNNGG 2 cut(s) 913, 1158
Bsc4I CCNNNNNNNGG 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 563, 642
Bse3DI GCAATG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 913, 1158
BseGI GGATG 4 cut(s) 646, 811, 961, 981
BseLI CCNNNNNNNGG 1 cut(s) 706
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 1045
BseNI ACTGG 2 cut(s) 563, 642
BseYI CCCAGC 2 cut(s) 23, 243
BshFI GGCC 2 cut(s) 149, 912
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 913
BslFI GGGAC 1 cut(s) 930
BslI CCNNNNNNNGG 1 cut(s) 706
BsmAI GTCTC 1 cut(s) 980
BsmFI GGGAC 1 cut(s) 930
BsmI GAATGC 2 cut(s) 101, 804
BsnI GGCC 2 cut(s) 149, 912
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
BspACI CCGC 2 cut(s) 315, 432
BspANI GGCC 2 cut(s) 149, 912
BspCNI CTCAG 1 cut(s) 1044
BspLI GGNNCC 1 cut(s) 604
BspPI GGATC 2 cut(s) 691, 1216
BsrBI CCGCTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 279
BsrI ACTGG 2 cut(s) 563, 642
BssECI CCNNGG 2 cut(s) 913, 1158
BssMI GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
BssNAI GTATAC 1 cut(s) 409
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 1158
Bst1107I GTATAC 1 cut(s) 409
Bst2BI CACGAG 1 cut(s) 235
Bst4CI ACNGT 4 cut(s) 389, 842, 934, 1022
Bst6I CTCTTC 1 cut(s) 978
BstC8I GCNNGC 2 cut(s) 380, 468
BstDEI CTNAG 2 cut(s) 36, 1031
BstF5I GGATG 4 cut(s) 646, 811, 961, 981
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 2 cut(s) 466, 1135
BstKTI GATC 6 cut(s) 6, 174, 283, 699, 1224, 1246
BstMAI GTCTC 1 cut(s) 980
BstMBI GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
BstMWI GCNNNNNNNGC 1 cut(s) 438
BstNSI RCATGY 2 cut(s) 382, 859
BstSCI CCNGG 1 cut(s) 912
BstV2I GAAGAC 1 cut(s) 668
BstZ17I GTATAC 1 cut(s) 409
BsuRI GGCC 2 cut(s) 149, 912
BtsCI GGATG 4 cut(s) 646, 811, 961, 981
BtsIMutI CAGTG 2 cut(s) 394, 674
Cac8I GCNNGC 2 cut(s) 380, 468
CfoI GCGC 2 cut(s) 466, 1135
Csp6I GTAC 4 cut(s) 188, 635, 759, 864
CviAII CATG 6 cut(s) 221, 379, 543, 589, 856, 1289
CviQI GTAC 4 cut(s) 188, 635, 759, 864
DdeI CTNAG 2 cut(s) 36, 1031
DpnI GATC 6 cut(s) 5, 173, 282, 698, 1223, 1245
DpnII GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
DraI TTTAAA 1 cut(s) 583
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
Eco130I CCWWGG 1 cut(s) 1158
Eco147I AGGCCT 1 cut(s) 149
Eco47III AGCGCT 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 1158
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 1158
FaeI CATG 6 cut(s) 224, 382, 546, 592, 859, 1292
FalI AAGNNNNNCTT 4 cut(s) 353, 385, 450, 482
FaqI GGGAC 1 cut(s) 930
FatI CATG 6 cut(s) 220, 378, 542, 588, 855, 1288
FbaI TGATCA 2 cut(s) 3, 171
FblI GTMKAC 1 cut(s) 408
FokI GGATG 4 cut(s) 653, 818, 968, 988
FspBI CTAG 1 cut(s) 44
GlaI GCGC 2 cut(s) 465, 1134
GsaI CCCAGC 2 cut(s) 27, 247
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 2 cut(s) 149, 912
HapII CCGG 1 cut(s) 913
HhaI GCGC 2 cut(s) 466, 1135
Hin1II CATG 6 cut(s) 224, 382, 546, 592, 859, 1292
Hin6I GCGC 2 cut(s) 464, 1133
HinP1I GCGC 2 cut(s) 464, 1133
HincII GTYRAC 2 cut(s) 1165, 1183
HindII GTYRAC 2 cut(s) 1165, 1183
HindIII AAGCTT 1 cut(s) 1197
HinfI GANTC 7 cut(s) 217, 238, 288, 679, 918, 988, 1188
HpaI GTTAAC 1 cut(s) 1183
HpaII CCGG 1 cut(s) 913
HphI GGTGA 3 cut(s) 390, 1129, 1205
Hpy166II GTNNAC 4 cut(s) 409, 1064, 1165, 1183
Hpy188III TCNNGA 4 cut(s) 164, 370, 458, 1247
Hpy8I GTNNAC 4 cut(s) 409, 1064, 1165, 1183
Hpy99I CGWCG 1 cut(s) 1281
HpyAV CCTTC 4 cut(s) 69, 650, 803, 1090
HpyCH4III ACNGT 4 cut(s) 389, 842, 934, 1022
HpyCH4IV ACGT 1 cut(s) 1179
HpyF10VI GCNNNNNNNGC 1 cut(s) 438
HpyF3I CTNAG 2 cut(s) 36, 1031
HpySE526I ACGT 1 cut(s) 1179
Hsp92II CATG 6 cut(s) 224, 382, 546, 592, 859, 1292
HspAI GCGC 2 cut(s) 464, 1133
Ksp22I TGATCA 2 cut(s) 3, 171
KspAI GTTAAC 1 cut(s) 1183
Kzo9I GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
LmnI GCTCC 1 cut(s) 602
LweI GCATC 3 cut(s) 631, 946, 1227
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 1179
MaeIII GTNAC 5 cut(s) 514, 554, 619, 724, 934
MalI GATC 6 cut(s) 5, 173, 282, 698, 1223, 1245
MbiI CCGCTC 1 cut(s) 317
MboI GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
MfeI CAATTG 2 cut(s) 338, 1011
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 9 cut(s) 202, 321, 338, 393, 453, 523, 628, 741, 1011
MlyI GAGTC 4 cut(s) 247, 297, 912, 982
MmeI TCCRAC 2 cut(s) 330, 1273
MnlI CCTC 9 cut(s) 131, 139, 339, 488, 556, 664, 698, 997, 1197
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 2 cut(s) 197, 453
MspA1I CMGCKG 1 cut(s) 432
MspI CCGG 1 cut(s) 913
MspR9I CCNGG 1 cut(s) 914
MunI CAATTG 2 cut(s) 338, 1011
Mva1269I GAATGC 2 cut(s) 101, 804
MwoI GCNNNNNNNGC 1 cut(s) 438
NciI CCSGG 1 cut(s) 914
NdeII GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
NlaIII CATG 6 cut(s) 224, 382, 546, 592, 859, 1292
NlaIV GGNNCC 1 cut(s) 604
NmuCI GTSAC 2 cut(s) 514, 724
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 2 cut(s) 382, 859
PacI TTAATTAA 1 cut(s) 744
PaeI GCATGC 1 cut(s) 382
PceI AGGCCT 1 cut(s) 149
PctI GAATGC 2 cut(s) 101, 804
PdmI GAANNNNTTC 2 cut(s) 197, 453
PfeI GAWTC 3 cut(s) 217, 679, 1188
PleI GAGTC 4 cut(s) 246, 296, 912, 982
PpsI GAGTC 4 cut(s) 246, 296, 912, 982
PshBI ATTAAT 1 cut(s) 744
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 1179
PspFI CCCAGC 2 cut(s) 23, 243
PspN4I GGNNCC 1 cut(s) 604
RsaI GTAC 4 cut(s) 189, 636, 760, 865
RsaNI GTAC 4 cut(s) 188, 635, 759, 864
Sau3AI GATC 6 cut(s) 3, 171, 280, 696, 1221, 1243
SchI GAGTC 4 cut(s) 247, 297, 912, 982
ScrFI CCNGG 1 cut(s) 914
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 3 cut(s) 631, 946, 1227
SphI GCATGC 1 cut(s) 382
Sse9I AATT 9 cut(s) 202, 321, 338, 393, 453, 523, 628, 741, 1011
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 2 cut(s) 315, 432
SspI AATATT 1 cut(s) 999
SspMI CTAG 1 cut(s) 44
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 912
StyI CCWWGG 1 cut(s) 1158
TaaI ACNGT 4 cut(s) 389, 842, 934, 1022
TaiI ACGT 1 cut(s) 1182
TaqI TCGA 1 cut(s) 163
TasI AATT 9 cut(s) 202, 321, 338, 393, 453, 523, 628, 741, 1011
TatI WGTACW 1 cut(s) 634
TfiI GAWTC 3 cut(s) 217, 679, 1188
TscAI CASTG 2 cut(s) 394, 681
TseFI GTSAC 2 cut(s) 514, 724
Tsp45I GTSAC 2 cut(s) 514, 724
TspDTI ATGAA 5 cut(s) 71, 209, 531, 822, 1040
TspRI CASTG 2 cut(s) 394, 681
VspI ATTAAT 1 cut(s) 744
XapI RAATTY 2 cut(s) 393, 453
XceI RCATGY 2 cut(s) 382, 859
XmiI GTMKAC 1 cut(s) 408
XmnI GAANNNNTTC 2 cut(s) 197, 453
XspI CTAG 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.