MD04G1015800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001472
Physical Location & Seq
Reverse (-)
1716906 .. 1718396
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1015800.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGTTTACTTTTCTGCAGAATACAAGCAGTGGATTTCATTCCGACGCTGTTAATGCTAATCCTTCATGTATGCATAGAATTAAACAAACCCATTTGGTGTCGGAGAGTATGATTCAGACTCGATGCAAATCTGGAAAAATTAGGAAAGATGAAGCATTGGGTTACTTCGATTCCATGATTCAAACCAAACCCTTGCCCTCTATTTGGACTTTCAATTGTTTGTTTGGGGCACTCTCCAAGATGAAGGAATATTCTGCTGTGGTTTCTATGTGTAAACAGTTGATGGGTTGCGCTCAATTCCGACCTGATGTCTGTACAATCAATATTTTTCTGAATTGCTTGTGCCGTCTAAACCAGGTGGATTTGGGTTTCTCTGTTTTAGCAATTACCCTTAAATATGGTCTTAAACCCGATGCTCGTTCTTTAACTATTTTGCTTCACGGGCTTTGCATGTACAATTCCCTGGCTGAGGCAATGGAGCTGTTCAAGTTAATAGAAGAGAAAGGATACGAATGCAATGAAATAACTTATGCTACCATGATCAATGGACTATGCAAAGCTGGGATAACTTCTAAGGCACTCGAGATACTAATGAAGATGTGGGAAGATGGAAGGTTGAAGCCCAACCAAGATTGCTACAATCCCATCATCGATAGCCTCTGTAAAGAAAGACGAATAGAAGAGGCCTTGACCCTATTTCGAGATATGATCAGCAAAAATGTTGTACCGAATGTTTTCAGTTATACCACATTGATTCATGGGTTATGCAACACGAGCCGCTGGGAAGAAGTGCTCTCTCTTTTTGGAGGAATGGTAGATCAAGGAGTCAAGCCTAATGTTGTCACCTACAACACTCTAATTTATGCGTTGTGCCAATCGGGGAGGTTTGAAAAAGCCAAGAGCTTCTTGATTTGCATGCTTAACAGTGGAATTTCACCCGATGTATACACATATAATGTTCTTATCGGCCATCTTTGCAAGGAAAAAAGAATTCATGAAGCGCTTACTCTGTTTGAAAAGATGACAACGAATGACCTAAAGCCTGATGTTGTCACTTTTAATTCCTTGATTTCTGCTTCATGCAAGTCTGGTAACTGGGAGGAAGGTGTGAGGTTATTTAAAACCATGACTGGTTATGGAGTCCTGCCTAATATTGTTACATACAATTCTGTACTGGATGCTTTATGCAAGGAAGGGAAGACAGCAGAGGCGCTGAATCTTGTGGAAGAAATGATTTTTAGGGAGGAAAAGCCTAATGTTGTGACTTACAACTCCTTAATTAATGGATTGTGCCGTACCAACCAATGGAGAGAAGCCACAAGGTTGTTTGACAAAATGTTATGCCATGGAATCTTACCTAATTGCATAACTTTGAAGATACTTTCTGGTGCTCTCTGCAATGGAGGGATGACCGATGAGGTTCGCAAAGAATTTAAGTCGAGAATGGATGGAGCTTTGAAGATTTTTCCTAATGCTTCATGTGCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

497

Amino Acids

55.8

Weight (kDa)

8.21

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 102 - 150 4.5e-09 PPR repeat family
PPR_long PF17177 151 - 251 4.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 155 - 186 8.9e-07 PPR repeat family
TPR_24 PF23276 158 - 268 1.2e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 165 - 218 6.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 168 - 200 6e-08 PPR repeat
PPR_1 PF12854 206 - 236 2.3e-08 PPR repeat
PPR_2 PF13041 208 - 257 7.7e-17 PPR repeat family
PPR PF01535 212 - 241 2.8e-06 PPR repeat
PPR_long PF17177 227 - 365 2.3e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 232 - 287 1.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 239 - 271 3.4e-06 PPR repeat
PPR_2 PF13041 243 - 292 7.3e-17 PPR repeat family
PPR PF01535 246 - 276 4.9e-06 PPR repeat
PPR_3 PF13812 267 - 325 8.7e-11 Pentatricopeptide repeat domain
TPR_24 PF23276 273 - 375 4.7e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 275 - 306 5.6e-11 PPR repeat
PPR_2 PF13041 278 - 327 9.6e-18 PPR repeat family
PPR PF01535 281 - 311 1.5e-07 PPR repeat
PPR_1 PF12854 310 - 342 8.6e-11 PPR repeat
PPR_2 PF13041 315 - 362 4.5e-15 PPR repeat family
PPR PF01535 316 - 344 7.6e-06 PPR repeat
PPR_long PF17177 328 - 462 1.7e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 337 - 395 1.4e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 345 - 377 2.8e-10 PPR repeat
PPR_2 PF13041 348 - 397 2.1e-16 PPR repeat family
PPR PF01535 351 - 381 3.5e-07 PPR repeat
PPR_1 PF12854 380 - 411 3.5e-11 PPR repeat
PPR_2 PF13041 383 - 432 1.8e-18 PPR repeat family
PPR PF01535 386 - 412 1.1e-06 PPR repeat
PPR_1 PF12854 417 - 446 1.2e-12 PPR repeat
PPR_2 PF13041 418 - 467 1.5e-16 PPR repeat family
PPR PF01535 421 - 451 4.5e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1305
AccI GTMKAC 1 cut(s) 945
AciI CCGC 1 cut(s) 778
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 3 cut(s) 930, 990, 1430
AfaI GTAC 5 cut(s) 316, 455, 726, 1173, 1297
AfeI AGCGCT 1 cut(s) 1002
AfiI CCNNNNNNNGG 3 cut(s) 204, 469, 1305
AgsI TTSAA 8 cut(s) 182, 214, 487, 619, 890, 1016, 1375, 1459
AhdI GACNNNNNGTC 1 cut(s) 308
AjnI CCWGG 2 cut(s) 354, 462
AluBI AGCT 4 cut(s) 481, 560, 903, 1454
AluI AGCT 4 cut(s) 481, 560, 903, 1454
Alw21I GWGCWC 2 cut(s) 795, 1393
Ama87I CYCGRG 1 cut(s) 581
Aor51HI AGCGCT 1 cut(s) 1002
AoxI GGCC 2 cut(s) 684, 967
ApoI RAATTY 3 cut(s) 930, 990, 1430
AseI ATTAAT 1 cut(s) 1281
Asp700I GAANNNNTTC 1 cut(s) 734
AspLEI GCGC 3 cut(s) 293, 1003, 1213
AsuHPI GGTGA 2 cut(s) 835, 927
AvaI CYCGRG 1 cut(s) 581
BaeGI GKGCMC 1 cut(s) 232
BauI CACGAG 1 cut(s) 772
BbsI GAAGAC 1 cut(s) 1205
Bbv12I GWGCWC 2 cut(s) 795, 1393
BbvCI CCTCAGC 1 cut(s) 468
BccI CCATC 5 cut(s) 277, 602, 653, 978, 1442
BceAI ACGGC 2 cut(s) 330, 1278
BciT130I CCWGG 2 cut(s) 356, 464
BciVI GTATCC 1 cut(s) 500
BclI TGATCA 2 cut(s) 540, 708
BfmI CTRYAG 1 cut(s) 14
BfoI RGCGCY 2 cut(s) 1004, 1214
BfuI GTATCC 1 cut(s) 500
BisI GCNGC 1 cut(s) 778
BlsI GCNGC 1 cut(s) 779
Bme1390I CCNGG 2 cut(s) 356, 464
BmeRI GACNNNNNGTC 1 cut(s) 308
BmeT110I CYCGRG 1 cut(s) 581
BmrFI CCNGG 2 cut(s) 356, 464
BmrI ACTGGG 1 cut(s) 1105
BmsI GCATC 3 cut(s) 113, 403, 1168
BmuI ACTGGG 1 cut(s) 1105
BpiI GAAGAC 1 cut(s) 1205
Bpu10I CCTNAGC 1 cut(s) 468
Bsa29I ATCGAT 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 462, 1345
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 469, 1305
Bse1I ACTGG 3 cut(s) 1100, 1135, 1179
Bse3DI GCAATG 3 cut(s) 480, 523, 1405
Bse8I GATNNNNATC 1 cut(s) 127
BseBI CCWGG 2 cut(s) 356, 464
BseCI ATCGAT 1 cut(s) 651
BseDI CCNNGG 2 cut(s) 462, 1345
BseGI GGATG 3 cut(s) 1183, 1413, 1453
BseJI GATNNNNATC 1 cut(s) 127
BseLI CCNNNNNNNGG 3 cut(s) 204, 469, 1305
BseMI GCAATG 3 cut(s) 480, 523, 1405
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 3 cut(s) 1100, 1135, 1179
BseSI GKGCMC 1 cut(s) 232
BseYI CCCAGC 2 cut(s) 560, 780
BshFI GGCC 2 cut(s) 686, 969
BshVI ATCGAT 1 cut(s) 651
BsiHKAI GWGCWC 2 cut(s) 795, 1393
BsiHKCI CYCGRG 1 cut(s) 581
BslI CCNNNNNNNGG 3 cut(s) 204, 469, 1305
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 2 cut(s) 686, 969
BsoBI CYCGRG 1 cut(s) 581
Bsp1286I GDGCHC 3 cut(s) 232, 795, 1393
Bsp1407I TGTACA 2 cut(s) 314, 453
Bsp143I GATC 3 cut(s) 540, 708, 817
Bsp19I CCATGG 1 cut(s) 1345
BspACI CCGC 1 cut(s) 778
BspANI GGCC 2 cut(s) 686, 969
BspCNI CTCAG 1 cut(s) 460
BspDI ATCGAT 1 cut(s) 651
BspHI TCATGA 1 cut(s) 994
BspMAI CTGCAG 1 cut(s) 18
BsrDI GCAATG 3 cut(s) 480, 523, 1405
BsrGI TGTACA 2 cut(s) 314, 453
BsrI ACTGG 3 cut(s) 1100, 1135, 1179
BssECI CCNNGG 2 cut(s) 462, 1345
BssMI GATC 3 cut(s) 540, 708, 817
BssNAI GTATAC 1 cut(s) 946
BssSI CACGAG 1 cut(s) 772
BssT1I CCWWGG 1 cut(s) 1345
Bst1107I GTATAC 1 cut(s) 946
Bst2BI CACGAG 1 cut(s) 772
Bst2UI CCWGG 2 cut(s) 356, 464
Bst4CI ACNGT 2 cut(s) 279, 926
Bst6I CTCTTC 2 cut(s) 492, 675
BstAUI TGTACA 2 cut(s) 314, 453
BstC8I GCNNGC 1 cut(s) 917
BstDEI CTNAG 2 cut(s) 468, 573
BstDSI CCRYGG 1 cut(s) 1345
BstENI CCTNNNNNAGG 1 cut(s) 467
BstF5I GGATG 3 cut(s) 1183, 1413, 1453
BstH2I RGCGCY 2 cut(s) 1004, 1214
BstHHI GCGC 3 cut(s) 293, 1003, 1213
BstKTI GATC 3 cut(s) 543, 711, 820
BstMBI GATC 3 cut(s) 540, 708, 817
BstMWI GCNNNNNNNGC 5 cut(s) 53, 442, 774, 975, 1481
BstNI CCWGG 2 cut(s) 356, 464
BstNSI RCATGY 2 cut(s) 454, 919
BstSCI CCNGG 2 cut(s) 354, 462
BstSFI CTRYAG 1 cut(s) 14
BstSLI GKGCMC 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 1205
BstZ17I GTATAC 1 cut(s) 946
Bsu15I ATCGAT 1 cut(s) 651
BsuI GTATCC 1 cut(s) 500
BsuRI GGCC 2 cut(s) 686, 969
BsuTUI ATCGAT 1 cut(s) 651
BtgI CCRYGG 1 cut(s) 1345
BtsCI GGATG 3 cut(s) 1183, 1413, 1453
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 2 cut(s) 34, 931
Cac8I GCNNGC 1 cut(s) 917
CciI TCATGA 1 cut(s) 994
CfoI GCGC 3 cut(s) 293, 1003, 1213
ClaI ATCGAT 1 cut(s) 651
CseI GACGC 1 cut(s) 53
CsiI ACCWGGT 1 cut(s) 354
Csp6I GTAC 5 cut(s) 315, 454, 725, 1172, 1296
CviQI GTAC 5 cut(s) 315, 454, 725, 1172, 1296
DdeI CTNAG 2 cut(s) 468, 573
DpnI GATC 3 cut(s) 542, 710, 819
DpnII GATC 3 cut(s) 540, 708, 817
DraI TTTAAA 1 cut(s) 1120
DriI GACNNNNNGTC 1 cut(s) 308
EaeI YGGCCR 1 cut(s) 967
Eam1104I CTCTTC 2 cut(s) 492, 675
Eam1105I GACNNNNNGTC 1 cut(s) 308
EarI CTCTTC 2 cut(s) 492, 675
Eco130I CCWWGG 1 cut(s) 1345
Eco147I AGGCCT 1 cut(s) 686
Eco47III AGCGCT 1 cut(s) 1002
Eco88I CYCGRG 1 cut(s) 581
EcoNI CCTNNNNNAGG 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 990
EcoRII CCWGG 2 cut(s) 354, 462
EcoT14I CCWWGG 1 cut(s) 1345
EcoT22I ATGCAT 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 1345
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FbaI TGATCA 2 cut(s) 540, 708
FblI GTMKAC 1 cut(s) 945
Fnu4HI GCNGC 1 cut(s) 778
FokI GGATG 3 cut(s) 1190, 1420, 1460
Fsp4HI GCNGC 1 cut(s) 778
GlaI GCGC 3 cut(s) 292, 1002, 1212
GluI GCNGC 1 cut(s) 778
GsaI CCCAGC 2 cut(s) 564, 784
HaeII RGCGCY 2 cut(s) 1004, 1214
HaeIII GGCC 2 cut(s) 686, 969
HgaI GACGC 1 cut(s) 53
HhaI GCGC 3 cut(s) 293, 1003, 1213
Hin6I GCGC 3 cut(s) 291, 1001, 1211
HinP1I GCGC 3 cut(s) 291, 1001, 1211
HinfI GANTC 9 cut(s) 112, 118, 170, 178, 754, 825, 1140, 1216, 1350
HphI GGTGA 2 cut(s) 835, 927
Hpy166II GTNNAC 3 cut(s) 6, 275, 946
Hpy188I TCNGA 5 cut(s) 43, 103, 117, 302, 333
Hpy188III TCNNGA 6 cut(s) 132, 583, 701, 907, 995, 1440
Hpy8I GTNNAC 3 cut(s) 6, 275, 946
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 5 cut(s) 72, 238, 606, 1097, 1187
HpyCH4III ACNGT 2 cut(s) 279, 926
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 442, 774, 975, 1481
HpyF3I CTNAG 2 cut(s) 468, 573
HspAI GCGC 3 cut(s) 291, 1001, 1211
Ksp22I TGATCA 2 cut(s) 540, 708
Kzo9I GATC 3 cut(s) 540, 708, 817
LmnI GCTCC 2 cut(s) 478, 1451
LweI GCATC 3 cut(s) 113, 403, 1168
MabI ACCWGGT 1 cut(s) 354
MaeIII GTNAC 6 cut(s) 161, 841, 1051, 1091, 1156, 1261
MalI GATC 3 cut(s) 542, 710, 819
MboI GATC 3 cut(s) 540, 708, 817
MboII GAAGA 9 cut(s) 509, 607, 617, 692, 797, 1210, 1238, 1387, 1471
MfeI CAATTG 1 cut(s) 214
MhlI GDGCHC 3 cut(s) 232, 795, 1393
MlyI GAGTC 3 cut(s) 112, 834, 1149
MmeI TCCRAC 3 cut(s) 66, 81, 325
Mph1103I ATGCAT 1 cut(s) 75
MroXI GAANNNNTTC 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 780
MspR9I CCNGG 2 cut(s) 356, 464
MunI CAATTG 1 cut(s) 214
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 2 cut(s) 356, 464
MwoI GCNNNNNNNGC 5 cut(s) 53, 442, 774, 975, 1481
NcoI CCATGG 1 cut(s) 1345
NdeII GATC 3 cut(s) 540, 708, 817
NmuCI GTSAC 3 cut(s) 841, 1051, 1261
NsiI ATGCAT 1 cut(s) 75
NspI RCATGY 2 cut(s) 454, 919
PacI TTAATTAA 1 cut(s) 1281
PaeI GCATGC 1 cut(s) 919
PaeR7I CTCGAG 1 cut(s) 581
PagI TCATGA 1 cut(s) 994
PceI AGGCCT 1 cut(s) 686
PctI GAATGC 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 734
PfeI GAWTC 6 cut(s) 112, 170, 178, 754, 1216, 1350
PflMI CCANNNNNTGG 1 cut(s) 1305
PkrI GCNGC 1 cut(s) 779
PleI GAGTC 3 cut(s) 112, 833, 1148
PpsI GAGTC 3 cut(s) 112, 833, 1148
PshBI ATTAAT 1 cut(s) 1281
Psp6I CCWGG 2 cut(s) 354, 462
PspFI CCCAGC 2 cut(s) 560, 780
PspGI CCWGG 2 cut(s) 354, 462
PstI CTGCAG 1 cut(s) 18
RsaI GTAC 5 cut(s) 316, 455, 726, 1173, 1297
RsaNI GTAC 5 cut(s) 315, 454, 725, 1172, 1296
SatI GCNGC 1 cut(s) 778
Sau3AI GATC 3 cut(s) 540, 708, 817
SchI GAGTC 3 cut(s) 112, 834, 1149
ScrFI CCNGG 2 cut(s) 356, 464
SduI GDGCHC 3 cut(s) 232, 795, 1393
SexAI ACCWGGT 1 cut(s) 354
SfaNI GCATC 3 cut(s) 113, 403, 1168
SfcI CTRYAG 1 cut(s) 14
Sfr274I CTCGAG 1 cut(s) 581
SlaI CTCGAG 1 cut(s) 581
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
SphI GCATGC 1 cut(s) 919
SseBI AGGCCT 1 cut(s) 686
SsiI CCGC 1 cut(s) 778
SspI AATATT 3 cut(s) 251, 325, 1153
StuI AGGCCT 1 cut(s) 686
StyD4I CCNGG 2 cut(s) 354, 462
StyI CCWWGG 1 cut(s) 1345
TaaI ACNGT 2 cut(s) 279, 926
TaqI TCGA 6 cut(s) 121, 168, 582, 651, 700, 1439
TaqII GACCGA 1 cut(s) 1427
TatI WGTACW 3 cut(s) 314, 453, 1171
TauI GCSGC 1 cut(s) 780
TfiI GAWTC 6 cut(s) 112, 170, 178, 754, 1216, 1350
TscAI CASTG 2 cut(s) 34, 931
TseFI GTSAC 3 cut(s) 841, 1051, 1261
Tsp45I GTSAC 3 cut(s) 841, 1051, 1261
TspRI CASTG 2 cut(s) 34, 931
Van91I CCANNNNNTGG 1 cut(s) 1305
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 1 cut(s) 467
XapI RAATTY 3 cut(s) 930, 990, 1430
XceI RCATGY 2 cut(s) 454, 919
XhoI CTCGAG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 945
XmnI GAANNNNTTC 1 cut(s) 734
Zsp2I ATGCAT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.