MD04G1016200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001472
Physical Location & Seq
Reverse (-)
1750539 .. 1752127
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1016200.v1.1.491

Sequence Viewer

Length: 1545 bp
ATGAAGATAAAGACGATAACCACGAGACTTCCTAACTCAACAGGTAATCCTTCATTCATATTTACCTTTCTGCACAAAAAAGATGGATTTCATTCCGCTGCTGGTAATGCTAATGCTACTAGTACAGATAGATTTAAACAACCCCATTTGGAATCGGACAATTTGATTCAAACTCGATGCAAATCCGGAAAAATTAGGAAAGATGAAGCATTGGGTTACTTCGATTCCATGATTCAAACCAAACCCTTGCCATCTATTTGGACTTTCAATTGTTTGTTTGGGGCACTCTCCAAGATGAAGCAGTATTCTGCTGTCGTTTCTATGTGTAAACAGTTGCTGGGTTGCGCTCAATTCCGACCTGAGGTGGGTACATTGAATATTCTTATGAATTGCTTGTGCTGTCTGAATCAGGTGGATTTGGGTTTCTCTGTTTTAGCAATTACCCTTAAATATGGTCTTAATCCCGATGCTCATACTATAAATACTTTGCTCCATGGACTTTGCAAGTACCGTTCCCTGGCCGAGGCAATGGAGTTCTTCCAAGAAATAGAAATGAAAGGATACAAATGCGATGAAATCACTTATGCTACCATGATCAATGGACTATGCAAAGCTGGGAAAACTTCTAAGGCGCTCGAGATACTTACAAAGATGTGGGAAGATGGAATGTTCAAGCCCAACCAAGAATGCTACAATCCAATCACTGATAGCCTCTGTAAAGAAAGACGAATAGACGAGGCCTTGATCCTATTTCGAGATATGATCAGCAAAAGTGTTGTACCGAATGTTTTCAATTATAACACATTGATTCATGGGTTATGCAACACGAGTCGCTGGGAAGAAGTGCTCTCTCTTTTTGAAGGAATGGTAGATCACGGAGTCAAGCCTAATGTTGTCACCTACAACACACTAATTTATGCATTGTGCCAATCGGGGAGGATGGAAAAGGCCAAGAGCTACTTGATTTGCATGCTTAACAGTGGAATTTCACCAGATGTATACACATATAGTATTCTTATCAGTAGTCTTTGCAAGGAAGAAAGAATTCAAGAAGCGCTTACTTTGTTGGAAAATATGACGACGAAAGGCATAAAGCCTGATGTTGTCACTTTTACTTCCTTGATTTCTGCATCATGCAAGTCTGGTAACTGGGAGGAAGGTGTGAGGTTATTCAAAACCATGATTGGTTATGGAGCCTTGCCTAATATTGTTACATACAGTGCTGTTCTGGATGCTTTATGCAAGGAAGGGAAGACAGCAGAGGCACTGAATCTTGTGGAAGAAATGATCAGTAGGGGGGAAAAGCCTAATGTTGTGACTTACAACTCCTTAATTAATGGATTGTGTCGTACCAACCAATGGAGAGAAGCCACAAGGTTGTTTGACAAAATGTTATGCCATGGAATCTTACCTAATCGCCTAACTTTGAAGATACTTTCGGGTGCTCTCTGCAATGGAGGGATGGCCGATGAGGTTCGCAAAGAATTTAAGGCGAGAATGGATGGAGCTTTGAAGATTTTTCCTAATGCTTCATGTGCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

515

Amino Acids

57.46

Weight (kDa)

8.51

Isoelectric Point (pI)

27.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 120 - 169 3.6e-08 PPR repeat family
PPR_1 PF12854 152 - 183 8.1e-06 PPR repeat
PPR_2 PF13041 159 - 204 2.8e-12 PPR repeat family
TPR_24 PF23276 176 - 287 1.1e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 186 - 218 1.6e-10 PPR repeat
PPR PF01535 193 - 222 4.8e-07 PPR repeat
PPR_2 PF13041 226 - 275 2.7e-12 PPR repeat family
PPR_long PF17177 239 - 383 8.3e-12 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 250 - 306 7.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 258 - 290 2e-06 PPR repeat
PPR_2 PF13041 261 - 310 8.6e-18 PPR repeat family
PPR PF01535 266 - 294 1.9e-06 PPR repeat
PPR_3 PF13812 284 - 342 1.2e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 292 - 393 2.6e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 293 - 318 1.4e-10 PPR repeat
PPR_2 PF13041 296 - 345 5e-18 PPR repeat family
PPR PF01535 299 - 329 1.3e-07 PPR repeat
PPR_1 PF12854 328 - 360 2.4e-11 PPR repeat
PPR PF01535 334 - 364 7.4e-06 PPR repeat
PPR_2 PF13041 338 - 380 8.2e-12 PPR repeat family
PPR_3 PF13812 355 - 413 3.6e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 362 - 394 1.8e-10 PPR repeat
PPR_2 PF13041 366 - 415 3.2e-14 PPR repeat family
PPR PF01535 369 - 398 1.3e-06 PPR repeat
PPR_long PF17177 386 - 492 4.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 398 - 429 1.8e-09 PPR repeat
PPR_2 PF13041 401 - 450 1.1e-18 PPR repeat family
PPR PF01535 404 - 433 6.1e-07 PPR repeat
PPR_3 PF13812 425 - 477 1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 433 - 464 4.5e-13 PPR repeat
PPR_2 PF13041 436 - 485 2.3e-16 PPR repeat family
PPR PF01535 439 - 469 4.7e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
AccB7I CCANNNNNTGG 1 cut(s) 1359
AccI GTMKAC 1 cut(s) 999
AccIII TCCGGA 1 cut(s) 185
AciI CCGC 1 cut(s) 96
AclWI GGATC 1 cut(s) 739
AcoI YGGCCR 2 cut(s) 519, 1464
AcsI RAATTY 3 cut(s) 984, 1044, 1484
AfaI GTAC 5 cut(s) 124, 370, 509, 780, 1351
AfeI AGCGCT 1 cut(s) 1056
AfiI CCNNNNNNNGG 5 cut(s) 361, 365, 517, 523, 1359
AhlI ACTAGT 1 cut(s) 119
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 3 cut(s) 614, 957, 1508
AluI AGCT 3 cut(s) 614, 957, 1508
Alw21I GWGCWC 2 cut(s) 849, 1447
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 739
AlwNI CAGNNNCTG 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 635
Aor13HI TCCGGA 1 cut(s) 185
Aor51HI AGCGCT 1 cut(s) 1056
AoxI GGCC 4 cut(s) 519, 738, 948, 1464
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 3 cut(s) 984, 1044, 1484
AseI ATTAAT 1 cut(s) 1335
Asp700I GAANNNNTTC 2 cut(s) 788, 1044
AspLEI GCGC 3 cut(s) 347, 634, 1057
AsuHPI GGTGA 2 cut(s) 889, 981
AvaI CYCGRG 1 cut(s) 635
AxyI CCTNAGG 1 cut(s) 360
BaeGI GKGCMC 1 cut(s) 286
BauI CACGAG 2 cut(s) 22, 826
BbsI GAAGAC 1 cut(s) 1259
Bbv12I GWGCWC 2 cut(s) 849, 1447
BbvI GCAGC 1 cut(s) 85
BccI CCATC 6 cut(s) 77, 259, 656, 934, 1456, 1496
BciT130I CCWGG 1 cut(s) 518
BciVI GTATCC 1 cut(s) 554
BclI TGATCA 3 cut(s) 594, 762, 1287
BcoDI GTCTC 1 cut(s) 19
BcuI ACTAGT 1 cut(s) 119
BfaI CTAG 1 cut(s) 120
BfoI RGCGCY 2 cut(s) 635, 1058
BfuI GTATCC 1 cut(s) 554
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
Bme1390I CCNGG 1 cut(s) 518
BmeT110I CYCGRG 1 cut(s) 635
BmiI GGNNCC 1 cut(s) 1195
BmrFI CCNGG 1 cut(s) 518
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 4 cut(s) 167, 457, 1139, 1222
BmuI ACTGGG 1 cut(s) 1159
BpiI GAAGAC 1 cut(s) 1259
BsaBI GATNNNNATC 1 cut(s) 181
BsaJI CCNNGG 4 cut(s) 493, 516, 522, 1399
BsaWI WCCGGW 1 cut(s) 185
Bsc4I CCNNNNNNNGG 5 cut(s) 361, 365, 517, 523, 1359
Bse1I ACTGG 1 cut(s) 1154
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 2 cut(s) 534, 1459
Bse8I GATNNNNATC 1 cut(s) 181
BseAI TCCGGA 1 cut(s) 185
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 4 cut(s) 493, 516, 522, 1399
BseGI GGATG 4 cut(s) 945, 1237, 1467, 1507
BseJI GATNNNNATC 1 cut(s) 181
BseLI CCNNNNNNNGG 5 cut(s) 361, 365, 517, 523, 1359
BseMI GCAATG 2 cut(s) 534, 1459
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 1154
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 1 cut(s) 85
BseYI CCCAGC 3 cut(s) 337, 614, 834
BsgI GTGCAG 1 cut(s) 56
BshFI GGCC 4 cut(s) 521, 740, 950, 1466
BsiHKAI GWGCWC 2 cut(s) 849, 1447
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 1 cut(s) 186
BslI CCNNNNNNNGG 5 cut(s) 361, 365, 517, 523, 1359
BsmAI GTCTC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 4 cut(s) 521, 740, 950, 1466
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 286, 849, 1447
Bsp13I TCCGGA 1 cut(s) 185
Bsp143I GATC 5 cut(s) 594, 744, 762, 871, 1287
Bsp19I CCATGG 2 cut(s) 493, 1399
BspACI CCGC 1 cut(s) 96
BspANI GGCC 4 cut(s) 521, 740, 950, 1466
BspCNI CTCAG 1 cut(s) 352
BspEI TCCGGA 1 cut(s) 185
BspLI GGNNCC 1 cut(s) 1195
BspPI GGATC 1 cut(s) 739
BsrDI GCAATG 2 cut(s) 534, 1459
BsrI ACTGG 1 cut(s) 1154
BssECI CCNNGG 4 cut(s) 493, 516, 522, 1399
BssMI GATC 5 cut(s) 594, 744, 762, 871, 1287
BssNAI GTATAC 1 cut(s) 1000
BssSI CACGAG 2 cut(s) 22, 826
BssT1I CCWWGG 2 cut(s) 493, 1399
Bst1107I GTATAC 1 cut(s) 1000
Bst2BI CACGAG 2 cut(s) 22, 826
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 4 cut(s) 333, 512, 980, 1220
BstC8I GCNNGC 1 cut(s) 971
BstDEI CTNAG 2 cut(s) 360, 627
BstDSI CCRYGG 2 cut(s) 493, 1399
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 4 cut(s) 945, 1237, 1467, 1507
BstH2I RGCGCY 2 cut(s) 635, 1058
BstHHI GCGC 3 cut(s) 347, 634, 1057
BstKTI GATC 5 cut(s) 597, 747, 765, 874, 1290
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 5 cut(s) 594, 744, 762, 871, 1287
BstMWI GCNNNNNNNGC 2 cut(s) 107, 1535
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 973
BstSCI CCNGG 1 cut(s) 516
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 85
BstV2I GAAGAC 1 cut(s) 1259
BstXI CCANNNNNNTGG 1 cut(s) 258
BstZ17I GTATAC 1 cut(s) 1000
Bsu36I CCTNAGG 1 cut(s) 360
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 4 cut(s) 521, 740, 950, 1466
BtgI CCRYGG 2 cut(s) 493, 1399
BtgZI GCGATG 1 cut(s) 585
BtsCI GGATG 4 cut(s) 945, 1237, 1467, 1507
BtsIMutI CAGTG 4 cut(s) 702, 985, 1225, 1265
Cac8I GCNNGC 1 cut(s) 971
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 3 cut(s) 347, 634, 1057
Csp6I GTAC 5 cut(s) 123, 369, 508, 779, 1350
CviAII CATG 9 cut(s) 229, 494, 592, 812, 970, 1134, 1180, 1400, 1533
CviQI GTAC 5 cut(s) 123, 369, 508, 779, 1350
DdeI CTNAG 2 cut(s) 360, 627
DpnI GATC 5 cut(s) 596, 746, 764, 873, 1289
DpnII GATC 5 cut(s) 594, 744, 762, 871, 1287
DraI TTTAAA 1 cut(s) 136
EaeI YGGCCR 2 cut(s) 519, 1464
Eco130I CCWWGG 2 cut(s) 493, 1399
Eco147I AGGCCT 1 cut(s) 740
Eco47III AGCGCT 1 cut(s) 1056
Eco81I CCTNAGG 1 cut(s) 360
Eco88I CYCGRG 1 cut(s) 635
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoRI GAATTC 1 cut(s) 1044
EcoRII CCWGG 1 cut(s) 516
EcoT14I CCWWGG 2 cut(s) 493, 1399
EcoT22I ATGCAT 1 cut(s) 922
ErhI CCWWGG 2 cut(s) 493, 1399
FaeI CATG 9 cut(s) 232, 497, 595, 815, 973, 1137, 1183, 1403, 1536
FalI AAGNNNNNCTT 4 cut(s) 944, 976, 1041, 1073
FatI CATG 9 cut(s) 228, 493, 591, 811, 969, 1133, 1179, 1399, 1532
FbaI TGATCA 3 cut(s) 594, 762, 1287
FblI GTMKAC 1 cut(s) 999
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 4 cut(s) 952, 1244, 1474, 1514
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 1 cut(s) 120
GlaI GCGC 3 cut(s) 346, 633, 1056
GluI GCNGC 1 cut(s) 99
GsaI CCCAGC 3 cut(s) 341, 618, 838
HaeII RGCGCY 2 cut(s) 635, 1058
HaeIII GGCC 4 cut(s) 521, 740, 950, 1466
HapII CCGG 1 cut(s) 186
HhaI GCGC 3 cut(s) 347, 634, 1057
Hin1II CATG 9 cut(s) 232, 497, 595, 815, 973, 1137, 1183, 1403, 1536
Hin6I GCGC 3 cut(s) 345, 632, 1055
HinP1I GCGC 3 cut(s) 345, 632, 1055
HpaII CCGG 1 cut(s) 186
HphI GGTGA 2 cut(s) 889, 981
Hpy166II GTNNAC 2 cut(s) 329, 1000
Hpy188I TCNGA 3 cut(s) 157, 356, 405
Hpy188III TCNNGA 6 cut(s) 186, 464, 637, 755, 1049, 1229
Hpy8I GTNNAC 2 cut(s) 329, 1000
Hpy99I CGWCG 1 cut(s) 1084
HpyAV CCTTC 4 cut(s) 60, 854, 1151, 1241
HpyCH4III ACNGT 4 cut(s) 333, 512, 980, 1220
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 1535
HpyF3I CTNAG 2 cut(s) 360, 627
Hsp92II CATG 9 cut(s) 232, 497, 595, 815, 973, 1137, 1183, 1403, 1536
HspAI GCGC 3 cut(s) 345, 632, 1055
Kpn2I TCCGGA 1 cut(s) 185
Ksp22I TGATCA 3 cut(s) 594, 762, 1287
Kzo9I GATC 5 cut(s) 594, 744, 762, 871, 1287
LmnI GCTCC 3 cut(s) 495, 1193, 1505
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 4 cut(s) 167, 457, 1139, 1222
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 6 cut(s) 215, 895, 1105, 1145, 1210, 1315
MalI GATC 5 cut(s) 596, 746, 764, 873, 1289
MboI GATC 5 cut(s) 594, 744, 762, 871, 1287
MboII GAAGA 9 cut(s) 16, 529, 671, 851, 1049, 1264, 1292, 1441, 1525
MfeI CAATTG 1 cut(s) 268
MhlI GDGCHC 3 cut(s) 286, 849, 1447
MlyI GAGTC 2 cut(s) 838, 888
MmeI TCCRAC 2 cut(s) 379, 1047
Mph1103I ATGCAT 1 cut(s) 922
MroI TCCGGA 1 cut(s) 185
MroXI GAANNNNTTC 2 cut(s) 788, 1044
MseI TTAA 7 cut(s) 135, 447, 459, 975, 1331, 1335, 1488
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 518
MunI CAATTG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 1 cut(s) 518
MwoI GCNNNNNNNGC 2 cut(s) 107, 1535
NcoI CCATGG 2 cut(s) 493, 1399
NdeII GATC 5 cut(s) 594, 744, 762, 871, 1287
NlaIII CATG 9 cut(s) 232, 497, 595, 815, 973, 1137, 1183, 1403, 1536
NlaIV GGNNCC 1 cut(s) 1195
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 3 cut(s) 895, 1105, 1315
NsiI ATGCAT 1 cut(s) 922
NspI RCATGY 1 cut(s) 973
PacI TTAATTAA 1 cut(s) 1335
PaeI GCATGC 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 635
PceI AGGCCT 1 cut(s) 740
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 692
PdmI GAANNNNTTC 2 cut(s) 788, 1044
PfeI GAWTC 8 cut(s) 152, 166, 224, 232, 406, 808, 1270, 1404
PflMI CCANNNNNTGG 1 cut(s) 1359
PkrI GCNGC 1 cut(s) 100
PleI GAGTC 2 cut(s) 837, 887
PpsI GAGTC 2 cut(s) 837, 887
PshBI ATTAAT 1 cut(s) 1335
PsiI TTATAA 1 cut(s) 798
Psp6I CCWGG 1 cut(s) 516
PspFI CCCAGC 3 cut(s) 337, 614, 834
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 1 cut(s) 1195
PstNI CAGNNNCTG 1 cut(s) 337
RsaI GTAC 5 cut(s) 124, 370, 509, 780, 1351
RsaNI GTAC 5 cut(s) 123, 369, 508, 779, 1350
SaqAI TTAA 7 cut(s) 135, 447, 459, 975, 1331, 1335, 1488
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 5 cut(s) 594, 744, 762, 871, 1287
SchI GAGTC 2 cut(s) 838, 888
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 3 cut(s) 286, 849, 1447
SfaNI GCATC 4 cut(s) 167, 457, 1139, 1222
Sfr274I CTCGAG 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
SpeI ACTAGT 1 cut(s) 119
SphI GCATGC 1 cut(s) 973
SseBI AGGCCT 1 cut(s) 740
SsiI CCGC 1 cut(s) 96
SspI AATATT 2 cut(s) 379, 1207
SspMI CTAG 1 cut(s) 120
StuI AGGCCT 1 cut(s) 740
StyD4I CCNGG 1 cut(s) 516
StyI CCWWGG 2 cut(s) 493, 1399
TaaI ACNGT 4 cut(s) 333, 512, 980, 1220
TaqI TCGA 4 cut(s) 175, 222, 636, 754
TatI WGTACW 1 cut(s) 122
TfiI GAWTC 8 cut(s) 152, 166, 224, 232, 406, 808, 1270, 1404
Tru1I TTAA 7 cut(s) 135, 447, 459, 975, 1331, 1335, 1488
Tru9I TTAA 7 cut(s) 135, 447, 459, 975, 1331, 1335, 1488
TscAI CASTG 4 cut(s) 709, 985, 1225, 1272
TseFI GTSAC 3 cut(s) 895, 1105, 1315
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 3 cut(s) 895, 1105, 1315
TspGWI ACGGA 1 cut(s) 891
TspRI CASTG 4 cut(s) 709, 985, 1225, 1272
Van91I CCANNNNNTGG 1 cut(s) 1359
VspI ATTAAT 1 cut(s) 1335
XagI CCTNNNNNAGG 1 cut(s) 521
XapI RAATTY 3 cut(s) 984, 1044, 1484
XceI RCATGY 1 cut(s) 973
XhoI CTCGAG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 999
XmnI GAANNNNTTC 2 cut(s) 788, 1044
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.