pycom04g01740

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001472
Physical Location & Seq
Reverse (-)
1614871 .. 1619344
4474 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g01740.1

Sequence Viewer

Length: 1680 bp
ATGGTATGCCATGGAATCTCACCTGATTGCATAACTTTGATGATACTTTCCAGTGCTCTCTGCAATGGAGGGATACCAGATGAGGTTCGCCAAGAATTTAAGGCGAGAATAGATGGAGCTTTGAAGCTTGAGAGAAACTTGACTAGTTTAACGAAACTGAAAAGGACGATGAAAAGGATAACTACAAGAACTCCATTCCCAGGTAATCTTCCTTCTTTCATTTTTACCATTCTGCAGAATCGAAGTAGGGGATTTCACTCCGACGCTGTTAATGCTAATCCTACTTGTACGCATACAATTAAACAAACCCATTTGGTGTCGGAGAGTGTGATTCAGACTCGATGCAAATCTGGAAAAATTAGGAAAGATGAAGCATTGGGTTACTTTAATTCCATGATTCAAACCAAACCCTTGCCCTCTACTTGGACTTTCAATTGTTTGTTGGGGGCACTCTCCAAGATGAAGCAATATTCTGCTGTGGTTTCTCTGTGTAAACAGTTGATGGGTTGCGCTCATTTCCGACCTGATGTCAGTACAATGACTATTTTTCTGAATTGCTTGTGCCGTCTGAACCGGGTGGATTTGGGTTTCCCTGTTTTAGCAATTACTCTTAAATATGGTCTTCAACCCGATGCTCATTGTATAAATACTTTACTTCACGGGCTTTGCAAGTACAGTTCCTGGGCCGAGGCAATGGAGTTGTTCAAGTTAATAGAAGAGAAGGGATGCACATGCAATGAAATCACTTATGCTACCATGATCAATGGACTATGCAAGGCTGGGAAAACTTTTAAGGCACTCGAGATACTTACGAATATGTTGGAAGATGGAAGGTTTAAGCCAAACCAAGATTGCTACAATCCAATCATCGATAGCTTGTGTAAAGAAAGCCGAATGGATGATGCCTTGACCCTATTTCGAGATATGATCAGCAAAAGTGTTGTACCGGATATTTTCAGTTATAATTCTTTGATTCATGGGTTATGCAACATGAGTCGCTGGGAAGAAGTGTTCCCTCTTTTTGAAGGAATGGAAGATCATGGAGTCGGGCCAGATGTTGTCACCTACACCACTTTAATTTATGCATTGTGCCAATCGGGGAGGTTGGAAAAAGCCAAGAGCTTCTTGATTTGCATGCTTAACAGTGGAATTTCACCCAATGTATACACCTATAATGTTCTTATGAACACTCTTTGCAAGGAAGAAAGATTTCAAGAAGCGCTTACTCTGTTGGAAAATATGACAACGAAAGGCATAAAACCTGATGTTGTCACTTTTAATCCCTTGATTTCTGCTTTATGCAAGTCTGGTAACTGGGAAGAAGGTGTGAGGTTATTCAAAACCATGATTGGTTATGGAGCACTGCCTGATATTGTTACATACAATTCTGTACTGGATGCTTTATGCAAGGAAGGGAAGACAACAGAGGCACTGAATCTTGTCGACGAAATGTTCCGTAGTGGGGAAAAGCCTGATGTTGTGACTTACAACTCTTTAATTAAAGGATTGTGTCGTACCAGGCAATGGAAAGAAGCCACAAGGTTGTTTGACGAAATGGTATGCCATGGAATCTCACCTGATTTCATAACTTTGAAGATACTTTCGGGTGCTCTCTGCAATGAAGTGATGACTGATGAGGTTCGCGAAGAATTTAAGGCGAGAATAGATGGAGCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

560

Amino Acids

62.71

Weight (kDa)

7.89

Isoelectric Point (pI)

27.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 175 - 224 1e-07 PPR repeat family
PPR_2 PF13041 210 - 259 1.2e-13 PPR repeat family
TPR_24 PF23276 231 - 341 2.2e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 241 - 273 1.2e-09 PPR repeat
PPR PF01535 248 - 277 4.4e-06 PPR repeat
PPR_1 PF12854 279 - 309 1.9e-08 PPR repeat
PPR_2 PF13041 281 - 330 3.5e-15 PPR repeat family
PPR_1 PF12854 313 - 345 2.1e-06 PPR repeat
PPR_2 PF13041 316 - 365 3.6e-16 PPR repeat family
PPR_3 PF13812 340 - 398 1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 348 - 379 6.5e-10 PPR repeat
TPR_24 PF23276 349 - 448 8e-10 Fungal tetratrico peptide repeats
PPR_2 PF13041 351 - 400 3.3e-17 PPR repeat family
PPR PF01535 354 - 384 6.1e-07 PPR repeat
PPR_1 PF12854 383 - 415 5e-09 PPR repeat
PPR_2 PF13041 390 - 435 2.1e-14 PPR repeat family
PPR_3 PF13812 410 - 468 1.6e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 417 - 449 3e-11 PPR repeat
PPR_2 PF13041 421 - 463 6e-12 PPR repeat family
PPR PF01535 424 - 453 1e-06 PPR repeat
PPR_1 PF12854 453 - 484 3.2e-10 PPR repeat
PPR_2 PF13041 456 - 505 1.5e-19 PPR repeat family
PPR PF01535 459 - 488 1.5e-07 PPR repeat
PPR_3 PF13812 480 - 533 1.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 488 - 520 1.3e-12 PPR repeat
PPR_2 PF13041 491 - 540 1e-16 PPR repeat family
PPR PF01535 494 - 524 3.5e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 963
AccB7I CCANNNNNTGG 1 cut(s) 1524
AccI GTMKAC 2 cut(s) 1164, 1443
AccII CGCG 1 cut(s) 1644
AcsI RAATTY 3 cut(s) 95, 1149, 1649
AfaI GTAC 6 cut(s) 289, 535, 674, 945, 1392, 1516
AfeI AGCGCT 1 cut(s) 1221
AfiI CCNNNNNNNGG 4 cut(s) 200, 423, 1462, 1524
AgsI TTSAA 9 cut(s) 124, 401, 433, 626, 706, 1025, 1214, 1339, 1594
AhdI GACNNNNNGTC 1 cut(s) 527
AhlI ACTAGT 1 cut(s) 143
AjnI CCWGG 3 cut(s) 199, 680, 1517
AloI GAACNNNNNNTCC 2 cut(s) 995, 1027
AluBI AGCT 5 cut(s) 119, 127, 876, 1122, 1673
AluI AGCT 5 cut(s) 119, 127, 876, 1122, 1673
Alw21I GWGCWC 3 cut(s) 58, 1363, 1612
AlwNI CAGNNNCTG 1 cut(s) 681
Ama87I CYCGRG 1 cut(s) 800
Aor51HI AGCGCT 1 cut(s) 1221
AoxI GGCC 2 cut(s) 684, 1049
ApoI RAATTY 3 cut(s) 95, 1149, 1649
Asp700I GAANNNNTTC 1 cut(s) 1209
AspLEI GCGC 2 cut(s) 512, 1222
AspS9I GGNCC 2 cut(s) 684, 1049
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 4 cut(s) 12, 1054, 1146, 1566
AvaI CYCGRG 1 cut(s) 800
BaeGI GKGCMC 1 cut(s) 451
BarI GAAGNNNNNNTAC 2 cut(s) 196, 228
BbsI GAAGAC 2 cut(s) 614, 1424
Bbv12I GWGCWC 3 cut(s) 58, 1363, 1612
BccI CCATC 4 cut(s) 107, 496, 821, 1661
BceAI ACGGC 1 cut(s) 549
BciT130I CCWGG 3 cut(s) 201, 682, 1519
BciVI GTATCC 1 cut(s) 66
BclI TGATCA 2 cut(s) 759, 927
BcnI CCSGG 1 cut(s) 575
BcuI ACTAGT 1 cut(s) 143
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 233
BfoI RGCGCY 1 cut(s) 1223
BfuI GTATCC 1 cut(s) 66
Bme1390I CCNGG 4 cut(s) 201, 575, 682, 1519
BmeRI GACNNNNNGTC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 800
BmgT120I GGNCC 2 cut(s) 684, 1049
BmrFI CCNGG 4 cut(s) 201, 575, 682, 1519
BmrI ACTGGG 1 cut(s) 1324
BmsI GCATC 5 cut(s) 332, 622, 716, 892, 1387
BmuI ACTGGG 1 cut(s) 1324
BpiI GAAGAC 2 cut(s) 614, 1424
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 575
Bsa29I ATCGAT 1 cut(s) 870
BsaBI GATNNNNATC 1 cut(s) 346
BsaJI CCNNGG 5 cut(s) 10, 199, 681, 687, 1564
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 423, 1462, 1524
Bse1I ACTGG 3 cut(s) 51, 1319, 1398
Bse3DI GCAATG 5 cut(s) 70, 699, 742, 1529, 1624
Bse8I GATNNNNATC 1 cut(s) 346
BseBI CCWGG 3 cut(s) 201, 682, 1519
BseCI ATCGAT 1 cut(s) 870
BseDI CCNNGG 5 cut(s) 10, 199, 681, 687, 1564
BseGI GGATG 3 cut(s) 731, 904, 1402
BseJI GATNNNNATC 1 cut(s) 346
BseLI CCNNNNNNNGG 4 cut(s) 200, 423, 1462, 1524
BseMI GCAATG 5 cut(s) 70, 699, 742, 1529, 1624
BseNI ACTGG 3 cut(s) 51, 1319, 1398
BseSI GKGCMC 1 cut(s) 451
BseYI CCCAGC 2 cut(s) 779, 999
Bsh1236I CGCG 1 cut(s) 1644
BshFI GGCC 2 cut(s) 686, 1051
BshVI ATCGAT 1 cut(s) 870
BsiHKAI GWGCWC 3 cut(s) 58, 1363, 1612
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 2 cut(s) 574, 947
BslI CCNNNNNNNGG 4 cut(s) 200, 423, 1462, 1524
BsnI GGCC 2 cut(s) 686, 1051
BsoBI CYCGRG 1 cut(s) 800
Bsp1286I GDGCHC 4 cut(s) 58, 451, 1363, 1612
Bsp143I GATC 3 cut(s) 759, 927, 1036
Bsp19I CCATGG 2 cut(s) 10, 1564
Bsp68I TCGCGA 1 cut(s) 1644
BspANI GGCC 2 cut(s) 686, 1051
BspDI ATCGAT 1 cut(s) 870
BspFNI CGCG 1 cut(s) 1644
BspMAI CTGCAG 1 cut(s) 237
BsrDI GCAATG 5 cut(s) 70, 699, 742, 1529, 1624
BsrI ACTGG 3 cut(s) 51, 1319, 1398
BssECI CCNNGG 5 cut(s) 10, 199, 681, 687, 1564
BssMI GATC 3 cut(s) 759, 927, 1036
BssNAI GTATAC 1 cut(s) 1165
BssT1I CCWWGG 2 cut(s) 10, 1564
Bst1107I GTATAC 1 cut(s) 1165
Bst2UI CCWGG 3 cut(s) 201, 682, 1519
Bst4CI ACNGT 3 cut(s) 498, 677, 1145
Bst6I CTCTTC 1 cut(s) 711
BstC8I GCNNGC 1 cut(s) 1136
BstDSI CCRYGG 2 cut(s) 10, 1564
BstF5I GGATG 3 cut(s) 731, 904, 1402
BstFNI CGCG 1 cut(s) 1644
BstH2I RGCGCY 1 cut(s) 1223
BstHHI GCGC 2 cut(s) 512, 1222
BstKTI GATC 3 cut(s) 762, 930, 1039
BstMBI GATC 3 cut(s) 759, 927, 1036
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 3 cut(s) 201, 682, 1519
BstNSI RCATGY 2 cut(s) 735, 1138
BstSCI CCNGG 4 cut(s) 199, 573, 680, 1517
BstSFI CTRYAG 1 cut(s) 233
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 1 cut(s) 1644
BstV2I GAAGAC 2 cut(s) 614, 1424
BstZ17I GTATAC 1 cut(s) 1165
Bsu15I ATCGAT 1 cut(s) 870
BsuI GTATCC 1 cut(s) 66
BsuRI GGCC 2 cut(s) 686, 1051
BsuTUI ATCGAT 1 cut(s) 870
BtgI CCRYGG 2 cut(s) 10, 1564
BtsCI GGATG 3 cut(s) 731, 904, 1402
BtsI GCAGTG 1 cut(s) 1361
BtsIMutI CAGTG 4 cut(s) 58, 1150, 1361, 1430
BtuMI TCGCGA 1 cut(s) 1644
Cac8I GCNNGC 1 cut(s) 1136
CaiI CAGNNNCTG 1 cut(s) 681
CfoI GCGC 2 cut(s) 512, 1222
Cfr13I GGNCC 2 cut(s) 684, 1049
ClaI ATCGAT 1 cut(s) 870
CseI GACGC 1 cut(s) 272
Csp6I GTAC 6 cut(s) 288, 534, 673, 944, 1391, 1515
CviQI GTAC 6 cut(s) 288, 534, 673, 944, 1391, 1515
DpnI GATC 3 cut(s) 761, 929, 1038
DpnII GATC 3 cut(s) 759, 927, 1036
DriI GACNNNNNGTC 1 cut(s) 527
Eam1104I CTCTTC 1 cut(s) 711
Eam1105I GACNNNNNGTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 711
Eco130I CCWWGG 2 cut(s) 10, 1564
Eco47III AGCGCT 1 cut(s) 1221
Eco88I CYCGRG 1 cut(s) 800
EcoRII CCWGG 3 cut(s) 199, 680, 1517
EcoT14I CCWWGG 2 cut(s) 10, 1564
EcoT22I ATGCAT 1 cut(s) 1087
ErhI CCWWGG 2 cut(s) 10, 1564
FalI AAGNNNNNCTT 4 cut(s) 1109, 1141, 1206, 1238
FbaI TGATCA 2 cut(s) 759, 927
FblI GTMKAC 2 cut(s) 1164, 1443
FokI GGATG 3 cut(s) 738, 911, 1409
FspBI CTAG 1 cut(s) 144
GlaI GCGC 2 cut(s) 511, 1221
GsaI CCCAGC 2 cut(s) 783, 1003
HaeII RGCGCY 1 cut(s) 1223
HaeIII GGCC 2 cut(s) 686, 1051
HapII CCGG 2 cut(s) 574, 947
HgaI GACGC 1 cut(s) 272
HhaI GCGC 2 cut(s) 512, 1222
Hin6I GCGC 2 cut(s) 510, 1220
HinP1I GCGC 2 cut(s) 510, 1220
HincII GTYRAC 1 cut(s) 1444
HindII GTYRAC 1 cut(s) 1444
HindIII AAGCTT 1 cut(s) 125
HpaII CCGG 2 cut(s) 574, 947
HphI GGTGA 4 cut(s) 12, 1054, 1146, 1566
Hpy166II GTNNAC 3 cut(s) 494, 1165, 1444
Hpy188I TCNGA 6 cut(s) 262, 322, 336, 521, 552, 570
Hpy188III TCNNGA 6 cut(s) 351, 802, 920, 1126, 1214, 1643
Hpy8I GTNNAC 3 cut(s) 494, 1165, 1444
Hpy99I CGWCG 2 cut(s) 266, 1448
HpyAV CCTTC 6 cut(s) 222, 715, 825, 1019, 1316, 1406
HpyCH4III ACNGT 3 cut(s) 498, 677, 1145
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HspAI GCGC 2 cut(s) 510, 1220
Ksp22I TGATCA 2 cut(s) 759, 927
Kzo9I GATC 3 cut(s) 759, 927, 1036
LmnI GCTCC 3 cut(s) 116, 1358, 1670
LweI GCATC 5 cut(s) 332, 622, 716, 892, 1387
MaeI CTAG 1 cut(s) 144
MaeIII GTNAC 6 cut(s) 380, 1060, 1270, 1310, 1375, 1480
MalI GATC 3 cut(s) 761, 929, 1038
MboI GATC 3 cut(s) 759, 927, 1036
MfeI CAATTG 1 cut(s) 433
MhlI GDGCHC 4 cut(s) 58, 451, 1363, 1612
MlyI GAGTC 3 cut(s) 331, 1003, 1053
MmeI TCCRAC 6 cut(s) 285, 300, 544, 801, 1086, 1212
MnlI CCTC 9 cut(s) 62, 76, 427, 682, 1026, 1095, 1323, 1420, 1630
Mph1103I ATGCAT 1 cut(s) 1087
MroXI GAANNNNTTC 1 cut(s) 1209
MspI CCGG 2 cut(s) 574, 947
MspR9I CCNGG 4 cut(s) 201, 575, 682, 1519
MunI CAATTG 1 cut(s) 433
MvaI CCWGG 3 cut(s) 201, 682, 1519
MvnI CGCG 1 cut(s) 1644
MwoI GCNNNNNNNGC 1 cut(s) 272
NciI CCSGG 1 cut(s) 575
NcoI CCATGG 2 cut(s) 10, 1564
NdeII GATC 3 cut(s) 759, 927, 1036
NmeAIII GCCGAG 1 cut(s) 712
NmuCI GTSAC 3 cut(s) 1060, 1270, 1480
NruI TCGCGA 1 cut(s) 1644
NsiI ATGCAT 1 cut(s) 1087
NspI RCATGY 2 cut(s) 735, 1138
PacI TTAATTAA 1 cut(s) 1500
PaeI GCATGC 1 cut(s) 1138
PaeR7I CTCGAG 1 cut(s) 800
PdmI GAANNNNTTC 1 cut(s) 1209
PfeI GAWTC 7 cut(s) 15, 238, 331, 397, 973, 1435, 1569
PflMI CCANNNNNTGG 1 cut(s) 1524
PleI GAGTC 3 cut(s) 331, 1002, 1052
PpsI GAGTC 3 cut(s) 331, 1002, 1052
PsiI TTATAA 1 cut(s) 963
Psp6I CCWGG 3 cut(s) 199, 680, 1517
PspFI CCCAGC 2 cut(s) 779, 999
PspGI CCWGG 3 cut(s) 199, 680, 1517
PspPI GGNCC 2 cut(s) 684, 1049
PstI CTGCAG 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 681
RruI TCGCGA 1 cut(s) 1644
RsaI GTAC 6 cut(s) 289, 535, 674, 945, 1392, 1516
RsaNI GTAC 6 cut(s) 288, 534, 673, 944, 1391, 1515
SalI GTCGAC 1 cut(s) 1442
Sau3AI GATC 3 cut(s) 759, 927, 1036
Sau96I GGNCC 2 cut(s) 684, 1049
SchI GAGTC 3 cut(s) 331, 1003, 1053
ScrFI CCNGG 4 cut(s) 201, 575, 682, 1519
SduI GDGCHC 4 cut(s) 58, 451, 1363, 1612
SfaNI GCATC 5 cut(s) 332, 622, 716, 892, 1387
SfcI CTRYAG 1 cut(s) 233
Sfr274I CTCGAG 1 cut(s) 800
SlaI CTCGAG 1 cut(s) 800
SmlI CTYRAG 2 cut(s) 128, 800
SmoI CTYRAG 2 cut(s) 128, 800
SpeI ACTAGT 1 cut(s) 143
SphI GCATGC 1 cut(s) 1138
SspI AATATT 1 cut(s) 470
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 4 cut(s) 199, 573, 680, 1517
StyI CCWWGG 2 cut(s) 10, 1564
TaaI ACNGT 3 cut(s) 498, 677, 1145
TaqI TCGA 6 cut(s) 241, 340, 801, 870, 919, 1443
TatI WGTACW 3 cut(s) 533, 672, 1390
TfiI GAWTC 7 cut(s) 15, 238, 331, 397, 973, 1435, 1569
TscAI CASTG 4 cut(s) 58, 1150, 1368, 1437
TseFI GTSAC 3 cut(s) 1060, 1270, 1480
Tsp45I GTSAC 3 cut(s) 1060, 1270, 1480
TspDTI ATGAA 9 cut(s) 185, 208, 384, 476, 753, 965, 1199, 1573, 1635
TspGWI ACGGA 1 cut(s) 1445
TspRI CASTG 4 cut(s) 58, 1150, 1368, 1437
Van91I CCANNNNNTGG 1 cut(s) 1524
XapI RAATTY 3 cut(s) 95, 1149, 1649
XceI RCATGY 2 cut(s) 735, 1138
XhoI CTCGAG 1 cut(s) 800
XmiI GTMKAC 2 cut(s) 1164, 1443
XmnI GAANNNNTTC 1 cut(s) 1209
XspI CTAG 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.