pycom04g01130

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001472
Physical Location & Seq
Reverse (-)
1024480 .. 1026378
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g01130.2

Sequence Viewer

Length: 1815 bp
ATGAAGATAAAGGTGATAACCACGAGACTTCCTAACTCAACAGATAGATTTAATCAAACCCATTCGGAATCGGACAATTTGATTCAAACTCGATGCAAATCTGGAAAGATTAGGAAAGATGAAGCATTCGGTTACTTCGATTCCATGATTCAAACCAAACCCTTGCCCTCTATTTGGACTTTCAATTGTTTGTTTGGGGCACTCTCCAAGATGAAGGAGTATTCTGCTGTGGTTTCTATGTGTAAACAGTTGATGGGTTGCGCTCAATTCCGACCTGATGTCTGTACAATGAATATTGTTCTGAATTGCTTGTGCCGTCTGAACCGGGTGGACTTGGGTTTCTCTGTTTTAGCAACTACCCTTAAATATGGTCTTCAACCCGATGCTCATTCTATAAATACTTTACTTCACGGGCTTTGCAAGTACAGTTCCTGGGCTGAGGCAATGGAGCTGTTCAAGTTAATAGAAGAGAAGGGATACACATGCAATGAAATCACTTATGCTACCATGATCAAAGGACTATGCAAGGCTGGGAAAACTTCTAAGGCACTTGAGATACTTATGAATATGTTGGAAGATGGAAGGTTCAAGCCCAAACAAGACTGCTACAATCCCATCATAAATAGCCTGTGTAAGGAAAGACAAGTAGATGAGGCCTTGACCCTATTTCGAGATATGATCAGCAAAAATGTTGTACCGGATATTATCACTTATAACACTTTGATTCATGGGTTATGCAACATGAGTCGCTGGGAAGAAGTGCTCTCTCTTTTTGAAGAAATGATCGATCAAGGAGTCAGGCCAGATGTTGTCACGTATAACACTCTAATTTATGCATTGTGCCAATCAGGGAGGTTGGAGAAAGCCAAGAGCTTCTTGATTTGCATGCTTAACAGTGGAATTTCACCCAATGTATACACATATAATGTTCTTATCAGCGGTCTTTGCAAGGAAGAAAGAATTCAAGAAGCACTTACTCTGTTGGAAAATATGACAACGAAAGGCATAAAGCCTGATGTTGTCACTTTTACTTCCTTGATTTCTGCATCATGCAAGTCTGGTAACTGGGAGGAAGGTGTGAGGTTCTTCAAAACCATGATTGGTTATGGAGCCTTGCCTAATATTGTTACATACAATGTTGTTCTGAATGCTTTATGCAAGAAAGGGAAGACAGCGGAGGCACTGAATCTTGTGGAAGAAATGATCAGTAGCGGGGAAAAGCCTGATGTTGTGACTTACAGCTCCTTAATTAATGGATTGTGCCGGACCAGCCAATTGAGAGAATCTACAAGGGTGTTTAATAGAATGCTAGATGAAGGTATTGCCCCAAATGTTATCACTTACAACAGTTTAATCCATGGCGTGTGCCGTACAGCTCAATGGGAAGAAGCCACAAGGTTGTTTGATGATATGGTAGGCCGGGGACTCTTGCCTAATACCGTTACTTTGGTAATACTTTTGGATGCTATCCGCAAAGGTGGGATGACAGAAGAGACTCACAAAATATTTGAGGCAACAATTGAATACGGTATGAAACTCAGCACAATAACTTGCAATATGCTGGTTAGTGAATATTGTTTGCATGGCAGAATGGATAAGGCGAAGGTGGTCTTGAACTTAATGGTGATTATGGGTTATGCGCCTGATATTGCTTCCTATAAAACCTTGGTCAACGGCTATATAAGCGTTGACAGAATCAGTGAAGTTTTGAGGCTTGTTAAAGAAATGATCCAAATAGGATTGATGCCTGATCTGGAAATGCAACAAACTTTGGGGCGTTTTCGCCGTAAAAGCCACATTGGATTAGCAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

605

Amino Acids

67.95

Weight (kDa)

8.32

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AccI GTMKAC 1 cut(s) 915
AciI CCGC 4 cut(s) 939, 1175, 1212, 1471
AclWI GGATC 1 cut(s) 1723
AcsI RAATTY 2 cut(s) 900, 960
AfaI GTAC 4 cut(s) 286, 425, 696, 1372
AfiI CCNNNNNNNGG 2 cut(s) 174, 634
AhdI GACNNNNNGTC 1 cut(s) 278
AjnI CCWGG 1 cut(s) 431
AluBI AGCT 4 cut(s) 451, 873, 1242, 1376
AluI AGCT 4 cut(s) 451, 873, 1242, 1376
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 2 cut(s) 19, 1487
AlwI GGATC 1 cut(s) 1723
AlwNI CAGNNNCTG 1 cut(s) 432
AoxI GGCC 3 cut(s) 654, 800, 1417
ApoI RAATTY 2 cut(s) 900, 960
AseI ATTAAT 1 cut(s) 1251
Asp700I GAANNNNTTC 1 cut(s) 960
AspLEI GCGC 2 cut(s) 263, 1642
AspS9I GGNCC 1 cut(s) 1266
AsuC2I CCSGG 2 cut(s) 326, 1421
AsuHPI GGTGA 3 cut(s) 25, 897, 1636
AvaII GGWCC 1 cut(s) 1266
BaeGI GKGCMC 1 cut(s) 202
BauI CACGAG 1 cut(s) 22
BbsI GAAGAC 2 cut(s) 365, 1175
Bbv12I GWGCWC 1 cut(s) 765
BbvCI CCTCAGC 1 cut(s) 438
BccI CCATC 3 cut(s) 247, 572, 623
BceAI ACGGC 4 cut(s) 300, 1353, 1690, 1770
BciT130I CCWGG 1 cut(s) 433
BciVI GTATCC 1 cut(s) 470
BclI TGATCA 3 cut(s) 510, 678, 1203
BcnI CCSGG 2 cut(s) 326, 1421
BcoDI GTCTC 2 cut(s) 19, 1487
BfaI CTAG 1 cut(s) 1310
BfuI GTATCC 1 cut(s) 470
Bme1390I CCNGG 3 cut(s) 326, 433, 1421
Bme18I GGWCC 1 cut(s) 1266
BmeRI GACNNNNNGTC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 1266
BmiI GGNNCC 1 cut(s) 1111
BmrFI CCNGG 3 cut(s) 326, 433, 1421
BmrI ACTGGG 1 cut(s) 1075
BmsI GCATC 5 cut(s) 83, 373, 1055, 1453, 1734
BmuI ACTGGG 1 cut(s) 1075
BpiI GAAGAC 2 cut(s) 365, 1175
Bpu10I CCTNAGC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 2 cut(s) 326, 1421
Bsa29I ATCGAT 1 cut(s) 786
BsaAI YACGTR 1 cut(s) 816
BsaBI GATNNNNATC 1 cut(s) 97
BsaJI CCNNGG 4 cut(s) 432, 1357, 1420, 1665
BsaWI WCCGGW 1 cut(s) 697
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 634
Bse1I ACTGG 1 cut(s) 1070
Bse3DI GCAATG 2 cut(s) 450, 493
Bse8I GATNNNNATC 1 cut(s) 97
BseBI CCWGG 1 cut(s) 433
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 4 cut(s) 432, 1357, 1420, 1665
BseGI GGATG 2 cut(s) 1468, 1488
BseJI GATNNNNATC 1 cut(s) 97
BseLI CCNNNNNNNGG 2 cut(s) 174, 634
BseMI GCAATG 2 cut(s) 450, 493
BseMII CTCAG 2 cut(s) 429, 1552
BseNI ACTGG 1 cut(s) 1070
BseSI GKGCMC 1 cut(s) 202
BseYI CCCAGC 2 cut(s) 530, 750
BshFI GGCC 3 cut(s) 656, 802, 1419
BshVI ATCGAT 1 cut(s) 786
BsiHKAI GWGCWC 1 cut(s) 765
BsiSI CCGG 4 cut(s) 325, 698, 1264, 1420
BslFI GGGAC 1 cut(s) 1437
BslI CCNNNNNNNGG 2 cut(s) 174, 634
BsmAI GTCTC 2 cut(s) 19, 1487
BsmFI GGGAC 1 cut(s) 1437
BsmI GAATGC 3 cut(s) 125, 1153, 1311
BsnI GGCC 3 cut(s) 656, 802, 1419
Bsp1286I GDGCHC 2 cut(s) 202, 765
Bsp1407I TGTACA 1 cut(s) 284
Bsp143I GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
Bsp19I CCATGG 1 cut(s) 1357
BspACI CCGC 4 cut(s) 939, 1175, 1212, 1471
BspANI GGCC 3 cut(s) 656, 802, 1419
BspCNI CTCAG 2 cut(s) 430, 1551
BspDI ATCGAT 1 cut(s) 786
BspLI GGNNCC 1 cut(s) 1111
BspPI GGATC 1 cut(s) 1723
BsrDI GCAATG 2 cut(s) 450, 493
BsrGI TGTACA 1 cut(s) 284
BsrI ACTGG 1 cut(s) 1070
BssECI CCNNGG 4 cut(s) 432, 1357, 1420, 1665
BssMI GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
BssNAI GTATAC 1 cut(s) 916
BssSI CACGAG 1 cut(s) 22
BssT1I CCWWGG 2 cut(s) 1357, 1665
Bst1107I GTATAC 1 cut(s) 916
Bst2BI CACGAG 1 cut(s) 22
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 6 cut(s) 249, 428, 896, 1349, 1441, 1529
Bst6I CTCTTC 2 cut(s) 462, 1485
BstAUI TGTACA 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 887
BstDEI CTNAG 3 cut(s) 438, 543, 1538
BstDSI CCRYGG 1 cut(s) 1357
BstENI CCTNNNNNAGG 1 cut(s) 632
BstF5I GGATG 2 cut(s) 1468, 1488
BstHHI GCGC 2 cut(s) 263, 1642
BstKTI GATC 7 cut(s) 513, 681, 786, 790, 1206, 1731, 1753
BstMAI GTCTC 2 cut(s) 19, 1487
BstMBI GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
BstMWI GCNNNNNNNGC 4 cut(s) 945, 1269, 1683, 1791
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 2 cut(s) 486, 889
BstSCI CCNGG 3 cut(s) 324, 431, 1419
BstSLI GKGCMC 1 cut(s) 202
BstV2I GAAGAC 2 cut(s) 365, 1175
BstZ17I GTATAC 1 cut(s) 916
Bsu15I ATCGAT 1 cut(s) 786
BsuI GTATCC 1 cut(s) 470
BsuRI GGCC 3 cut(s) 656, 802, 1419
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 1 cut(s) 1357
BtsCI GGATG 2 cut(s) 1468, 1488
BtsIMutI CAGTG 3 cut(s) 901, 1181, 1705
Cac8I GCNNGC 1 cut(s) 887
CaiI CAGNNNCTG 1 cut(s) 432
CfoI GCGC 2 cut(s) 263, 1642
Cfr13I GGNCC 1 cut(s) 1266
ClaI ATCGAT 1 cut(s) 786
Csp6I GTAC 4 cut(s) 285, 424, 695, 1371
CviQI GTAC 4 cut(s) 285, 424, 695, 1371
DdeI CTNAG 3 cut(s) 438, 543, 1538
DpnI GATC 7 cut(s) 512, 680, 785, 789, 1205, 1730, 1752
DpnII GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
DriI GACNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 462, 1485
Eam1105I GACNNNNNGTC 1 cut(s) 278
EarI CTCTTC 2 cut(s) 462, 1485
Eco130I CCWWGG 2 cut(s) 1357, 1665
Eco147I AGGCCT 1 cut(s) 656
Eco47I GGWCC 1 cut(s) 1266
EcoNI CCTNNNNNAGG 1 cut(s) 632
EcoRI GAATTC 1 cut(s) 960
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 1357, 1665
EcoT22I ATGCAT 1 cut(s) 838
ErhI CCWWGG 2 cut(s) 1357, 1665
FalI AAGNNNNNCTT 6 cut(s) 860, 892, 957, 989, 1595, 1627
FaqI GGGAC 1 cut(s) 1437
FauI CCCGC 1 cut(s) 1205
FbaI TGATCA 3 cut(s) 510, 678, 1203
FblI GTMKAC 1 cut(s) 915
FokI GGATG 2 cut(s) 1475, 1495
FspBI CTAG 1 cut(s) 1310
GlaI GCGC 2 cut(s) 262, 1641
GsaI CCCAGC 2 cut(s) 534, 754
HaeIII GGCC 3 cut(s) 656, 802, 1419
HapII CCGG 4 cut(s) 325, 698, 1264, 1420
HhaI GCGC 2 cut(s) 263, 1642
Hin6I GCGC 2 cut(s) 261, 1640
HinP1I GCGC 2 cut(s) 261, 1640
HincII GTYRAC 2 cut(s) 1672, 1690
HindII GTYRAC 2 cut(s) 1672, 1690
HpaII CCGG 4 cut(s) 325, 698, 1264, 1420
HphI GGTGA 3 cut(s) 25, 897, 1636
Hpy166II GTNNAC 5 cut(s) 245, 331, 916, 1672, 1690
Hpy188I TCNGA 6 cut(s) 67, 73, 272, 303, 321, 1146
Hpy188III TCNNGA 6 cut(s) 102, 671, 877, 965, 1612, 1754
Hpy8I GTNNAC 5 cut(s) 245, 331, 916, 1672, 1690
HpyAV CCTTC 6 cut(s) 208, 466, 576, 1067, 1310, 1597
HpyCH4III ACNGT 6 cut(s) 249, 428, 896, 1349, 1441, 1529
HpyCH4IV ACGT 1 cut(s) 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 945, 1269, 1683, 1791
HpyF3I CTNAG 3 cut(s) 438, 543, 1538
HpySE526I ACGT 1 cut(s) 815
HspAI GCGC 2 cut(s) 261, 1640
Ksp22I TGATCA 3 cut(s) 510, 678, 1203
Kzo9I GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
LmnI GCTCC 3 cut(s) 448, 1109, 1247
LweI GCATC 5 cut(s) 83, 373, 1055, 1453, 1734
MaeI CTAG 1 cut(s) 1310
MaeII ACGT 1 cut(s) 815
MaeIII GTNAC 7 cut(s) 131, 811, 1021, 1061, 1126, 1231, 1441
MalI GATC 7 cut(s) 512, 680, 785, 789, 1205, 1730, 1752
MboI GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
MfeI CAATTG 3 cut(s) 184, 1274, 1518
MhlI GDGCHC 2 cut(s) 202, 765
MlyI GAGTC 4 cut(s) 754, 804, 1419, 1489
MmeI TCCRAC 4 cut(s) 295, 552, 837, 963
MnlI CCTC 9 cut(s) 178, 433, 646, 846, 1063, 1074, 1171, 1504, 1704
Mph1103I ATGCAT 1 cut(s) 838
MroXI GAANNNNTTC 1 cut(s) 960
MspA1I CMGCKG 2 cut(s) 939, 1175
MspI CCGG 4 cut(s) 325, 698, 1264, 1420
MspR9I CCNGG 3 cut(s) 326, 433, 1421
MunI CAATTG 3 cut(s) 184, 1274, 1518
Mva1269I GAATGC 3 cut(s) 125, 1153, 1311
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 4 cut(s) 945, 1269, 1683, 1791
NciI CCSGG 2 cut(s) 326, 1421
NcoI CCATGG 1 cut(s) 1357
NdeII GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
NlaIV GGNNCC 1 cut(s) 1111
NmuCI GTSAC 3 cut(s) 811, 1021, 1231
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 2 cut(s) 486, 889
PacI TTAATTAA 1 cut(s) 1251
PaeI GCATGC 1 cut(s) 889
PceI AGGCCT 1 cut(s) 656
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 3 cut(s) 125, 1153, 1311
PdmI GAANNNNTTC 1 cut(s) 960
PfeI GAWTC 8 cut(s) 68, 82, 140, 148, 724, 1186, 1283, 1695
PleI GAGTC 4 cut(s) 753, 803, 1419, 1489
PpsI GAGTC 4 cut(s) 753, 803, 1419, 1489
Ppu21I YACGTR 1 cut(s) 816
PshBI ATTAAT 1 cut(s) 1251
PsiI TTATAA 1 cut(s) 714
Psp6I CCWGG 1 cut(s) 431
PspFI CCCAGC 2 cut(s) 530, 750
PspGI CCWGG 1 cut(s) 431
PspN4I GGNNCC 1 cut(s) 1111
PspPI GGNCC 1 cut(s) 1266
PstNI CAGNNNCTG 1 cut(s) 432
RsaI GTAC 4 cut(s) 286, 425, 696, 1372
RsaNI GTAC 4 cut(s) 285, 424, 695, 1371
Sau3AI GATC 7 cut(s) 510, 678, 783, 787, 1203, 1728, 1750
Sau96I GGNCC 1 cut(s) 1266
SchI GAGTC 4 cut(s) 754, 804, 1419, 1489
ScrFI CCNGG 3 cut(s) 326, 433, 1421
SduI GDGCHC 2 cut(s) 202, 765
SfaNI GCATC 5 cut(s) 83, 373, 1055, 1453, 1734
SinI GGWCC 1 cut(s) 1266
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
SphI GCATGC 1 cut(s) 889
SseBI AGGCCT 1 cut(s) 656
SsiI CCGC 4 cut(s) 939, 1175, 1212, 1471
SspI AATATT 4 cut(s) 295, 1123, 1506, 1574
SspMI CTAG 1 cut(s) 1310
StuI AGGCCT 1 cut(s) 656
StyD4I CCNGG 3 cut(s) 324, 431, 1419
StyI CCWWGG 2 cut(s) 1357, 1665
TaaI ACNGT 6 cut(s) 249, 428, 896, 1349, 1441, 1529
TaiI ACGT 1 cut(s) 818
TaqI TCGA 4 cut(s) 91, 138, 670, 786
TatI WGTACW 2 cut(s) 284, 423
TfiI GAWTC 8 cut(s) 68, 82, 140, 148, 724, 1186, 1283, 1695
TscAI CASTG 3 cut(s) 901, 1188, 1705
TseFI GTSAC 3 cut(s) 811, 1021, 1231
Tsp45I GTSAC 3 cut(s) 811, 1021, 1231
TspDTI ATGAA 9 cut(s) 17, 135, 227, 305, 504, 578, 716, 1329, 1547
TspRI CASTG 3 cut(s) 901, 1188, 1705
VpaK11BI GGWCC 1 cut(s) 1266
VspI ATTAAT 1 cut(s) 1251
XagI CCTNNNNNAGG 1 cut(s) 632
XapI RAATTY 2 cut(s) 900, 960
XceI RCATGY 2 cut(s) 486, 889
XmiI GTMKAC 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 960
XspI CTAG 1 cut(s) 1310
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.