MD04G1015900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001472
Physical Location & Seq
Reverse (-)
1726877 .. 1728880
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1015900.v1.1.491

Sequence Viewer

Length: 2004 bp
ATGAAGATGAAGGTGATAACCACGAGACTTCCTAACTCAACAGGTAATCCTTCATTCGTTTTTACCTTTCTGCACAAAAAAGATGGATTTCATTCCGCTGCTGGTAATGCTAATGCTACTAGTACAGATAGATTTAAACAAACCCATTTGGAATCGGACAATTTGATTCAAACTCGATGCAAATCTGGAAAAATTAGGAAAGATGAAGCATTGGGTTACTTCGATTCCATGATTCAAACCAAACCCTTGCCCTCTCTTGGGACTTTCAGTTGTTTGTTTGGGGCACTCTCCAAGATGAAGGAGTATTCTGCTGTCGTTTCTATGTGTAAACAGTTGATGTGTTGCGCTCAATTTCGACCTGATGTCTGTACAATGAATATTTTTCTGAATTGCTTGTGCCGTCTGAACCGGGTGGACTTGGGTTTCTCTGTTTTAGCAATTACCCTTAAATACGGTCTTCAACCCGATTCTTATTCTATAAGTATTTTACTTCACGGGCTTTGCAAGTACAGTTCCTGGGATGAGGCAATGGAGCTGTTCAAGTTAATAGAAGAGAAGGGATACACATGCAATGAAATCACTTATGCTACCATGATCAATGGACTATGCAAGGCTGGGAAAACTTCGAAGGCACTCGAGATACTAATGAAGATGTGGGAAGATGGAAGATTGAAGCCCAACCAAAATTGCTACAATCCAATTATGGATAGCCTCTGTAAAGAAAGACGAATAGACGACGCCTTGACCCTATTTCGAGATATGATCAGCAAAAGTGTTGTACCAGGTATTATCACTTATTCCACATTGATTCATGGGTTATGCAACACGAGCCGCTGGGAAGAAATGCTCTCTCTTTTTGAAGGAATGGTAGATCAAGGAGTCAAGCCTAATGTTATCACCTACAACACTCTAATTTATGCATTGTGCCAATCGGGGAGGATGGAAAAGGCCAAGAGCTACTTGATTTGCATGCTTAACAGTGGAATTTCACCAGATGTATACACATATAGTATTCTTATCAGCAGTCTTTGCAAGGAAGAAAAAATTCAAGAAGCGCTTACTCTGTTGGAAAATATGACAACGAAAGGCATAAAGCCTGATGTTGTCACTTTTACTTCCTTGATTTCTGCATCATGCAAGTCTGGTAACTGGGAGGAAGGTGTGAGGTTATTCAAAACCATGATTGGTTATGGAGCCTTGCCTAATATTGTTACATATAATTCTGTACTGGATGCTTTATGCAAGGAAGGGAAGACAGCAGAGGCACTGAATCTTGTGGAAGAAATGATCAGTAGGGGGGAAAAGCCTAATGTTGTGACTTACAGCTCCTTAATTAATGGATTGTGCCGGACCAGCCAATGGAGAGAAGCCACAAGGTTGTTTAATAGAATGCTGGATGAAGGTATTGCCCCAAATGTTATAACCTATAATAATATGATAAGTTCTCTATGCAAGGAGGGGAGGACCGAGGAAGCCCTCAGTATGTTAAAGTTAATGACTCAAAGAGGTCTGATACCTGATGTTATCACTTATAACAGTTTAATCCGTGGCATGTGCCGTACAGCTCAATGGGAAGAAGCCACAAGGTTGTTTGATGATATGGTAGGCCGGGGACTCTTGCCTGATACCGTTACTTTGGTAATACTTTTGGATGCTATCTGCAAAGGTAGGATGACAGAAGAGAATCACAAAATATTTGAGGCAACAATTGAATACGGTATGAAACTTAGTACAATAACTTGCAGTATGGTGGTTAATGAATATTGTTTGCATGGCAGAATGGATAAGGCAAAGGCGTTCTTGAACTTAATGGTGGTTATGGGTTATGCGCCTGATATTGCTTCCTATAAAACCTTGGTCAGCGGCTATATAAACGTTAACAGAATAGGTGAAGCTTTGAGGCTTGTTAAAGAAATGATCCAAATAGGATTGATGCCTGATCTAGAAATGCAACAAACTTTGGGGCGTTTTCGTCGTAAAAGGCATATTGGATTAGCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

668

Amino Acids

75.0

Weight (kDa)

8.5

Isoelectric Point (pI)

28.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 120 - 169 7.6e-11 PPR repeat family
PPR_2 PF13041 164 - 204 2.7e-09 PPR repeat family
TPR_24 PF23276 176 - 287 8.7e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 182 - 236 8.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 186 - 218 3.5e-09 PPR repeat
PPR PF01535 193 - 222 5.2e-07 PPR repeat
PPR_1 PF12854 224 - 254 2.5e-07 PPR repeat
PPR_2 PF13041 226 - 275 3.4e-14 PPR repeat family
PPR_3 PF13812 250 - 305 1.5e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 261 - 310 6.5e-16 PPR repeat family
PPR PF01535 264 - 294 3.3e-06 PPR repeat
PPR_3 PF13812 284 - 342 7.9e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 290 - 393 1.9e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 293 - 318 4.6e-10 PPR repeat
PPR_2 PF13041 296 - 345 2.6e-17 PPR repeat family
PPR PF01535 299 - 329 3.9e-07 PPR repeat
PPR_1 PF12854 328 - 360 6.3e-11 PPR repeat
PPR PF01535 334 - 364 9.2e-06 PPR repeat
PPR_2 PF13041 338 - 380 8.7e-12 PPR repeat family
PPR_long PF17177 346 - 445 4.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 355 - 413 1.2e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 362 - 394 2.5e-10 PPR repeat
PPR_2 PF13041 366 - 415 2.2e-14 PPR repeat family
PPR PF01535 369 - 398 1.8e-06 PPR repeat
PPR_1 PF12854 398 - 429 1.9e-10 PPR repeat
PPR_2 PF13041 401 - 450 7.1e-20 PPR repeat family
PPR PF01535 404 - 433 7.5e-08 PPR repeat
PPR_long PF17177 422 - 570 3.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 425 - 482 5.2e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 433 - 464 1.1e-10 PPR repeat
PPR PF01535 439 - 469 5.4e-08 PPR repeat
PPR_2 PF13041 443 - 477 2.3e-09 PPR repeat family
PPR_1 PF12854 468 - 499 5.8e-11 PPR repeat
PPR_2 PF13041 471 - 520 2.3e-18 PPR repeat family
PPR PF01535 474 - 504 6.1e-09 PPR repeat
PPR_1 PF12854 502 - 535 8e-11 PPR repeat
PPR_2 PF13041 506 - 555 2.8e-16 PPR repeat family
PPR PF01535 509 - 539 3.1e-07 PPR repeat
PPR_2 PF13041 577 - 624 7e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1421, 1533
AccB7I CCANNNNNTGG 1 cut(s) 1359
AccI GTMKAC 1 cut(s) 999
AciI CCGC 3 cut(s) 96, 832, 1863
AclI AACGTT 1 cut(s) 1875
AclWI GGATC 1 cut(s) 1912
AcsI RAATTY 2 cut(s) 984, 1044
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 7 cut(s) 124, 370, 509, 780, 1227, 1561, 1732
AfeI AGCGCT 1 cut(s) 1056
AfiI CCNNNNNNNGG 3 cut(s) 257, 258, 1359
AhdI GACNNNNNGTC 1 cut(s) 362
AhlI ACTAGT 1 cut(s) 119
AjnI CCWGG 2 cut(s) 515, 781
AluBI AGCT 5 cut(s) 535, 957, 1326, 1565, 1895
AluI AGCT 5 cut(s) 535, 957, 1326, 1565, 1895
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 1912
AlwNI CAGNNNCTG 1 cut(s) 516
Ama87I CYCGRG 1 cut(s) 635
Aor51HI AGCGCT 1 cut(s) 1056
AoxI GGCC 2 cut(s) 948, 1606
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 2 cut(s) 984, 1044
AseI ATTAAT 1 cut(s) 1335
Asp700I GAANNNNTTC 1 cut(s) 1044
AspLEI GCGC 3 cut(s) 347, 1057, 1831
AspS9I GGNCC 2 cut(s) 1350, 1464
AsuC2I CCSGG 2 cut(s) 410, 1610
AsuHPI GGTGA 4 cut(s) 25, 889, 981, 1901
AsuII TTCGAA 1 cut(s) 626
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 2 cut(s) 1350, 1464
BaeGI GKGCMC 1 cut(s) 286
BauI CACGAG 2 cut(s) 22, 826
BbsI GAAGAC 2 cut(s) 449, 1259
BbvI GCAGC 1 cut(s) 85
BccI CCATC 3 cut(s) 77, 656, 934
BceAI ACGGC 2 cut(s) 384, 1542
BciT130I CCWGG 2 cut(s) 517, 783
BciVI GTATCC 1 cut(s) 554
BclI TGATCA 3 cut(s) 594, 762, 1287
BcnI CCSGG 2 cut(s) 410, 1610
BcoDI GTCTC 1 cut(s) 19
BcuI ACTAGT 1 cut(s) 119
BfaI CTAG 2 cut(s) 120, 1943
BfoI RGCGCY 1 cut(s) 1058
BfuI GTATCC 1 cut(s) 554
BisI GCNGC 3 cut(s) 99, 832, 1864
BlsI GCNGC 3 cut(s) 100, 833, 1865
Bme1390I CCNGG 4 cut(s) 410, 517, 783, 1610
Bme18I GGWCC 2 cut(s) 1350, 1464
BmeRI GACNNNNNGTC 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 2 cut(s) 1350, 1464
BmiI GGNNCC 1 cut(s) 1195
BmrFI CCNGG 4 cut(s) 410, 517, 783, 1610
BmrI ACTGGG 1 cut(s) 1159
BmsI GCATC 5 cut(s) 167, 1139, 1222, 1642, 1923
BmuI ACTGGG 1 cut(s) 1159
BpiI GAAGAC 2 cut(s) 449, 1259
Bpu14I TTCGAA 1 cut(s) 626
BpuMI CCSGG 2 cut(s) 410, 1610
BsaBI GATNNNNATC 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 738
BsaJI CCNNGG 5 cut(s) 516, 1467, 1546, 1609, 1854
Bsc4I CCNNNNNNNGG 3 cut(s) 257, 258, 1359
Bse1I ACTGG 2 cut(s) 1154, 1233
Bse3DI GCAATG 2 cut(s) 534, 577
Bse8I GATNNNNATC 1 cut(s) 181
BseBI CCWGG 2 cut(s) 517, 783
BseDI CCNNGG 5 cut(s) 516, 1467, 1546, 1609, 1854
BseGI GGATG 6 cut(s) 526, 945, 1237, 1402, 1657, 1677
BseJI GATNNNNATC 1 cut(s) 181
BseLI CCNNNNNNNGG 3 cut(s) 257, 258, 1359
BseMI GCAATG 2 cut(s) 534, 577
BseMII CTCAG 1 cut(s) 1492
BseNI ACTGG 2 cut(s) 1154, 1233
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 1 cut(s) 85
BseYI CCCAGC 2 cut(s) 614, 834
BsgI GTGCAG 1 cut(s) 56
BshFI GGCC 2 cut(s) 950, 1608
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 3 cut(s) 409, 1348, 1609
BslFI GGGAC 2 cut(s) 274, 1626
BslI CCNNNNNNNGG 3 cut(s) 257, 258, 1359
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 2 cut(s) 274, 1626
BsmI GAATGC 1 cut(s) 1395
BsnI GGCC 2 cut(s) 950, 1608
BsoBI CYCGRG 1 cut(s) 635
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 1 cut(s) 286
Bsp1407I TGTACA 1 cut(s) 368
Bsp143I GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
BspACI CCGC 3 cut(s) 96, 832, 1863
BspANI GGCC 2 cut(s) 950, 1608
BspCNI CTCAG 1 cut(s) 1491
BspLI GGNNCC 1 cut(s) 1195
BspPI GGATC 1 cut(s) 1912
BspT104I TTCGAA 1 cut(s) 626
BsrDI GCAATG 2 cut(s) 534, 577
BsrGI TGTACA 1 cut(s) 368
BsrI ACTGG 2 cut(s) 1154, 1233
BssECI CCNNGG 5 cut(s) 516, 1467, 1546, 1609, 1854
BssMI GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
BssNAI GTATAC 1 cut(s) 1000
BssNI GRCGYC 1 cut(s) 738
BssSI CACGAG 2 cut(s) 22, 826
BssT1I CCWWGG 1 cut(s) 1854
Bst1107I GTATAC 1 cut(s) 1000
Bst2BI CACGAG 2 cut(s) 22, 826
Bst2UI CCWGG 2 cut(s) 517, 783
Bst4CI ACNGT 7 cut(s) 333, 455, 512, 980, 1538, 1630, 1718
Bst6I CTCTTC 2 cut(s) 546, 1674
BstACI GRCGYC 1 cut(s) 738
BstAPI GCANNNNNTGC 1 cut(s) 1029
BstAUI TGTACA 1 cut(s) 368
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 1 cut(s) 971
BstDEI CTNAG 2 cut(s) 1478, 1727
BstDSI CCRYGG 1 cut(s) 1546
BstF5I GGATG 6 cut(s) 526, 945, 1237, 1402, 1657, 1677
BstH2I RGCGCY 1 cut(s) 1058
BstHHI GCGC 3 cut(s) 347, 1057, 1831
BstKTI GATC 6 cut(s) 597, 765, 874, 1290, 1920, 1942
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
BstMWI GCNNNNNNNGC 4 cut(s) 107, 828, 1029, 1353
BstNI CCWGG 2 cut(s) 517, 783
BstNSI RCATGY 3 cut(s) 570, 973, 1555
BstSCI CCNGG 4 cut(s) 408, 515, 781, 1608
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 85
BstV2I GAAGAC 2 cut(s) 449, 1259
BstZ17I GTATAC 1 cut(s) 1000
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 2 cut(s) 950, 1608
BtgI CCRYGG 1 cut(s) 1546
BtsCI GGATG 6 cut(s) 526, 945, 1237, 1402, 1657, 1677
BtsIMutI CAGTG 2 cut(s) 985, 1265
Cac8I GCNNGC 1 cut(s) 971
CaiI CAGNNNCTG 1 cut(s) 516
CfoI GCGC 3 cut(s) 347, 1057, 1831
Cfr13I GGNCC 2 cut(s) 1350, 1464
CseI GACGC 1 cut(s) 746
CsiI ACCWGGT 1 cut(s) 781
Csp6I GTAC 7 cut(s) 123, 369, 508, 779, 1226, 1560, 1731
CviAII CATG 9 cut(s) 229, 567, 592, 812, 970, 1134, 1180, 1552, 1772
CviQI GTAC 7 cut(s) 123, 369, 508, 779, 1226, 1560, 1731
DdeI CTNAG 2 cut(s) 1478, 1727
DpnI GATC 6 cut(s) 596, 764, 873, 1289, 1919, 1941
DpnII GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
DraI TTTAAA 1 cut(s) 136
DriI GACNNNNNGTC 1 cut(s) 362
Eam1104I CTCTTC 2 cut(s) 546, 1674
Eam1105I GACNNNNNGTC 1 cut(s) 362
EarI CTCTTC 2 cut(s) 546, 1674
Eco130I CCWWGG 1 cut(s) 1854
Eco47I GGWCC 2 cut(s) 1350, 1464
Eco47III AGCGCT 1 cut(s) 1056
Eco88I CYCGRG 1 cut(s) 635
EcoRII CCWGG 2 cut(s) 515, 781
EcoT14I CCWWGG 1 cut(s) 1854
EcoT22I ATGCAT 1 cut(s) 922
ErhI CCWWGG 1 cut(s) 1854
FaeI CATG 9 cut(s) 232, 570, 595, 815, 973, 1137, 1183, 1555, 1775
FalI AAGNNNNNCTT 6 cut(s) 944, 976, 1041, 1073, 1784, 1816
FaqI GGGAC 2 cut(s) 274, 1626
FatI CATG 9 cut(s) 228, 566, 591, 811, 969, 1133, 1179, 1551, 1771
FbaI TGATCA 3 cut(s) 594, 762, 1287
FblI GTMKAC 1 cut(s) 999
Fnu4HI GCNGC 3 cut(s) 99, 832, 1864
FokI GGATG 6 cut(s) 533, 952, 1244, 1409, 1664, 1684
Fsp4HI GCNGC 3 cut(s) 99, 832, 1864
FspBI CTAG 2 cut(s) 120, 1943
GlaI GCGC 3 cut(s) 346, 1056, 1830
GluI GCNGC 3 cut(s) 99, 832, 1864
GsaI CCCAGC 2 cut(s) 618, 838
HaeII RGCGCY 1 cut(s) 1058
HaeIII GGCC 2 cut(s) 950, 1608
HapII CCGG 3 cut(s) 409, 1348, 1609
HgaI GACGC 1 cut(s) 746
HhaI GCGC 3 cut(s) 347, 1057, 1831
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 9 cut(s) 232, 570, 595, 815, 973, 1137, 1183, 1555, 1775
Hin6I GCGC 3 cut(s) 345, 1055, 1829
HinP1I GCGC 3 cut(s) 345, 1055, 1829
HincII GTYRAC 1 cut(s) 1879
HindII GTYRAC 1 cut(s) 1879
HindIII AAGCTT 1 cut(s) 1893
HpaI GTTAAC 1 cut(s) 1879
HpaII CCGG 3 cut(s) 409, 1348, 1609
HphI GGTGA 4 cut(s) 25, 889, 981, 1901
Hpy166II GTNNAC 4 cut(s) 329, 415, 1000, 1879
Hpy188I TCNGA 4 cut(s) 157, 387, 405, 1512
Hpy188III TCNNGA 6 cut(s) 186, 637, 755, 1049, 1801, 1943
Hpy8I GTNNAC 4 cut(s) 329, 415, 1000, 1879
Hpy99I CGWCG 2 cut(s) 740, 1977
HpyAV CCTTC 9 cut(s) 4, 60, 292, 550, 622, 854, 1151, 1241, 1394
HpyCH4III ACNGT 7 cut(s) 333, 455, 512, 980, 1538, 1630, 1718
HpyCH4IV ACGT 1 cut(s) 1875
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 828, 1029, 1353
HpyF3I CTNAG 2 cut(s) 1478, 1727
HpySE526I ACGT 1 cut(s) 1875
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 9 cut(s) 232, 570, 595, 815, 973, 1137, 1183, 1555, 1775
HspAI GCGC 3 cut(s) 345, 1055, 1829
Ksp22I TGATCA 3 cut(s) 594, 762, 1287
KspAI GTTAAC 1 cut(s) 1879
Kzo9I GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
LmnI GCTCC 3 cut(s) 532, 1193, 1331
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 5 cut(s) 167, 1139, 1222, 1642, 1923
MabI ACCWGGT 1 cut(s) 781
MaeI CTAG 2 cut(s) 120, 1943
MaeII ACGT 1 cut(s) 1875
MaeIII GTNAC 6 cut(s) 215, 1105, 1145, 1210, 1315, 1630
MalI GATC 6 cut(s) 596, 764, 873, 1289, 1919, 1941
MboI GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
MfeI CAATTG 1 cut(s) 1707
MhlI GDGCHC 1 cut(s) 286
MlyI GAGTC 3 cut(s) 888, 1492, 1608
MmeI TCCRAC 1 cut(s) 1047
Mph1103I ATGCAT 1 cut(s) 922
MroXI GAANNNNTTC 1 cut(s) 1044
MspA1I CMGCKG 3 cut(s) 98, 834, 1863
MspI CCGG 3 cut(s) 409, 1348, 1609
MspR9I CCNGG 4 cut(s) 410, 517, 783, 1610
MunI CAATTG 1 cut(s) 1707
Mva1269I GAATGC 1 cut(s) 1395
MvaI CCWGG 2 cut(s) 517, 783
MwoI GCNNNNNNNGC 4 cut(s) 107, 828, 1029, 1353
NciI CCSGG 2 cut(s) 410, 1610
NdeII GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
NlaIII CATG 9 cut(s) 232, 570, 595, 815, 973, 1137, 1183, 1555, 1775
NlaIV GGNNCC 1 cut(s) 1195
NmuCI GTSAC 2 cut(s) 1105, 1315
NsiI ATGCAT 1 cut(s) 922
NspI RCATGY 3 cut(s) 570, 973, 1555
NspV TTCGAA 1 cut(s) 626
PacI TTAATTAA 1 cut(s) 1335
PaeI GCATGC 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 635
PctI GAATGC 1 cut(s) 1395
PdmI GAANNNNTTC 1 cut(s) 1044
PfeI GAWTC 8 cut(s) 152, 166, 224, 232, 467, 808, 1270, 1684
PflMI CCANNNNNTGG 1 cut(s) 1359
PkrI GCNGC 3 cut(s) 100, 833, 1865
PleI GAGTC 3 cut(s) 887, 1492, 1608
PpsI GAGTC 3 cut(s) 887, 1492, 1608
PshBI ATTAAT 1 cut(s) 1335
PsiI TTATAA 2 cut(s) 1421, 1533
Psp1406I AACGTT 1 cut(s) 1875
Psp6I CCWGG 2 cut(s) 515, 781
PspFI CCCAGC 2 cut(s) 614, 834
PspGI CCWGG 2 cut(s) 515, 781
PspN4I GGNNCC 1 cut(s) 1195
PspPI GGNCC 2 cut(s) 1350, 1464
PstNI CAGNNNCTG 1 cut(s) 516
RsaI GTAC 7 cut(s) 124, 370, 509, 780, 1227, 1561, 1732
RsaNI GTAC 7 cut(s) 123, 369, 508, 779, 1226, 1560, 1731
SatI GCNGC 3 cut(s) 99, 832, 1864
Sau3AI GATC 6 cut(s) 594, 762, 871, 1287, 1917, 1939
Sau96I GGNCC 2 cut(s) 1350, 1464
SchI GAGTC 3 cut(s) 888, 1492, 1608
ScrFI CCNGG 4 cut(s) 410, 517, 783, 1610
SduI GDGCHC 1 cut(s) 286
SexAI ACCWGGT 1 cut(s) 781
SfaNI GCATC 5 cut(s) 167, 1139, 1222, 1642, 1923
Sfr274I CTCGAG 1 cut(s) 635
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 2 cut(s) 1350, 1464
SlaI CTCGAG 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
SpeI ACTAGT 1 cut(s) 119
SphI GCATGC 1 cut(s) 973
SsiI CCGC 3 cut(s) 96, 832, 1863
SspI AATATT 4 cut(s) 379, 1207, 1695, 1763
SspMI CTAG 2 cut(s) 120, 1943
StyD4I CCNGG 4 cut(s) 408, 515, 781, 1608
StyI CCWWGG 1 cut(s) 1854
TaaI ACNGT 7 cut(s) 333, 455, 512, 980, 1538, 1630, 1718
TaiI ACGT 1 cut(s) 1878
TaqI TCGA 6 cut(s) 175, 222, 355, 626, 636, 754
TaqII GACCGA 1 cut(s) 1481
TatI WGTACW 5 cut(s) 122, 368, 507, 1225, 1730
TauI GCSGC 2 cut(s) 834, 1866
TfiI GAWTC 8 cut(s) 152, 166, 224, 232, 467, 808, 1270, 1684
TscAI CASTG 2 cut(s) 985, 1272
TseFI GTSAC 2 cut(s) 1105, 1315
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 2 cut(s) 1105, 1315
TspGWI ACGGA 1 cut(s) 1535
TspRI CASTG 2 cut(s) 985, 1272
Van91I CCANNNNNTGG 1 cut(s) 1359
VpaK11BI GGWCC 2 cut(s) 1350, 1464
VspI ATTAAT 1 cut(s) 1335
XapI RAATTY 2 cut(s) 984, 1044
XbaI TCTAGA 1 cut(s) 1942
XceI RCATGY 3 cut(s) 570, 973, 1555
XhoI CTCGAG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 999
XmnI GAANNNNTTC 1 cut(s) 1044
XspI CTAG 2 cut(s) 120, 1943
Zsp2I ATGCAT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.