pycom04g01770

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001472
Physical Location & Seq
Reverse (-)
1631837 .. 1633006
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g01770.1

Sequence Viewer

Length: 1170 bp
ATGAAGATGAAGAGGATAACCACAAGACCTCCATCTCGAGGTAATCTTCCTTCTTTCATTTTTACCATTCTGCGGAATCCAAGTAGGGGATTTCATTCCGACGCTGTTAATGCTAATCCTACTTGTACGCATACAATTAAACGAACCCATTTGGTGTCGGAGAGTATGATTCAGACTCGATGCAAATCTGGAAAAATTAGGAAAGATGAAGCTTTGGGTTACTTTAATTCCATGATTCAAACCAAACCCTTGCCCTCTATTTGGACTTTCAATTGTTTGTTGGGGGCACTCTCCAAGATGAAGCAATATTCTGCTGTGGTTTCTATGTGTAAACAGTTGATGGGTTACGCTCATTTCCGACCTGATGTCAGTACAATGACTATTTTTATGAATTGCTTGTGCCGTCTGAACCGGGTGGATTTGGGTTTCCCTGTTTTAGCAATTACCCTTAAATACGGTCTTCAACCCAATGCTCATTCTATGAATACTTTGCTTCATGGGCTTTGCAAGTACAGTTCCCTGGCTGAGGCAATGGAGCTGTTCAAGTTAATAGAAGAGAAGGGATACAAATGCAATGAAATCACTTATGCTACCATGATCGATGGATTATGCAAAGTTGGGAAAATTTCTAAGGCAGTCGAGATTCTTAAGAAGATGTGGGAAGATGGAAGGTTTAAGCCAAACCAAGATTGCTACAATCCAATCATCGATAGCTTGTGTAAAGAAAGCCGAATGGACGATGCCTTGACCCTATTTCGAGATATGATCAGCAAAAGTGTTGTACCGGATATTATCAGTTACAACTCTTTGATTCATGGGTTATGCAACATGAGTCGCTGGGAAGAAGTGCTCCCTCTTTTTGAAGGAATGGAAGATCATGGAGTCAGGCCAGATGTTGTCACCTACACCACTTTAATTTATGCGTTGTGCCAATCGGGGAGGTTGGAAAAAGCCAAGAGCTTCTTGATTTGCATGCTTAACAGTGGAATTTCTCCTAATGTATGCACGTATAATGTTCTAATCAACGCGCTGTGCAATGATGAAAGAATTCAAGAAGCACTGACTCTGTTTGGAACAATGATTGAGAAACGTATAAAGCCTGACGTTGTCACTTTTACTTCCTTGATCGATCTCCTTCATGCAAGTCGGGTAACTGGGAGGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

390

Amino Acids

44.02

Weight (kDa)

9.08

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 121 - 170 8e-09 PPR repeat family
PPR_2 PF13041 156 - 205 1.1e-13 PPR repeat family
PPR_long PF17177 172 - 271 3.6e-06 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 177 - 288 8.3e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 185 - 237 6.7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 187 - 219 1.3e-10 PPR repeat
PPR PF01535 194 - 223 2.2e-07 PPR repeat
PPR_1 PF12854 225 - 255 1.2e-08 PPR repeat
PPR_2 PF13041 227 - 276 4.5e-15 PPR repeat family
PPR_long PF17177 248 - 376 8.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 259 - 291 4.9e-06 PPR repeat
PPR_2 PF13041 262 - 311 1.1e-16 PPR repeat family
PPR_3 PF13812 286 - 344 4.2e-14 Pentatricopeptide repeat domain
PPR_1 PF12854 294 - 325 9.6e-11 PPR repeat
PPR_2 PF13041 297 - 346 2.9e-17 PPR repeat family
PPR PF01535 300 - 330 3.8e-07 PPR repeat
PPR_1 PF12854 329 - 360 4.7e-10 PPR repeat
PPR_2 PF13041 332 - 378 2e-15 PPR repeat family
PPR PF01535 336 - 365 3.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1028
AciI CCGC 1 cut(s) 73
AcsI RAATTY 3 cut(s) 624, 987, 1047
AfaI GTAC 4 cut(s) 127, 373, 512, 783
AfiI CCNNNNNNNGG 5 cut(s) 38, 72, 86, 261, 526
AflII CTTAAG 1 cut(s) 647
AgsI TTSAA 6 cut(s) 239, 271, 464, 544, 863, 1052
AhdI GACNNNNNGTC 1 cut(s) 365
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 5 cut(s) 212, 538, 714, 960, 1165
AluI AGCT 5 cut(s) 212, 538, 714, 960, 1165
Alw21I GWGCWC 1 cut(s) 852
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 1 cut(s) 887
ApoI RAATTY 3 cut(s) 624, 987, 1047
Asp700I GAANNNNTTC 1 cut(s) 1047
AspLEI GCGC 1 cut(s) 1030
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 892
AvaI CYCGRG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 289
BarI GAAGNNNNNNTAC 2 cut(s) 34, 66
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 852
BbvCI CCTCAGC 1 cut(s) 525
BccI CCATC 4 cut(s) 40, 334, 596, 659
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 1 cut(s) 557
BclI TGATCA 1 cut(s) 765
BcnI CCSGG 1 cut(s) 413
BfrI CTTAAG 1 cut(s) 647
BfuI GTATCC 1 cut(s) 557
Bme1390I CCNGG 2 cut(s) 413, 521
BmeRI GACNNNNNGTC 1 cut(s) 365
BmeT110I CYCGRG 1 cut(s) 36
BmrFI CCNGG 2 cut(s) 413, 521
BmrI ACTGGG 1 cut(s) 1164
BmsI GCATC 2 cut(s) 170, 730
BmuI ACTGGG 1 cut(s) 1164
BpiI GAAGAC 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 525
BpuMI CCSGG 1 cut(s) 413
Bsa29I ATCGAT 3 cut(s) 600, 708, 1128
BsaAI YACGTR 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 184
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 1 cut(s) 784
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 5 cut(s) 38, 72, 86, 261, 526
Bse1I ACTGG 1 cut(s) 1159
Bse3DI GCAATG 3 cut(s) 537, 580, 1042
Bse8I GATNNNNATC 1 cut(s) 184
BseBI CCWGG 1 cut(s) 521
BseCI ATCGAT 3 cut(s) 600, 708, 1128
BseDI CCNNGG 1 cut(s) 519
BseJI GATNNNNATC 1 cut(s) 184
BseLI CCNNNNNNNGG 5 cut(s) 38, 72, 86, 261, 526
BseMI GCAATG 3 cut(s) 537, 580, 1042
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 1 cut(s) 1159
BseSI GKGCMC 1 cut(s) 289
BseYI CCCAGC 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 1028
BshFI GGCC 1 cut(s) 889
BshVI ATCGAT 3 cut(s) 600, 708, 1128
BsiHKAI GWGCWC 1 cut(s) 852
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 2 cut(s) 412, 785
BslI CCNNNNNNNGG 5 cut(s) 38, 72, 86, 261, 526
BsnI GGCC 1 cut(s) 889
BsoBI CYCGRG 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 289, 852
Bsp143I GATC 5 cut(s) 597, 765, 874, 1125, 1129
BspACI CCGC 1 cut(s) 73
BspANI GGCC 1 cut(s) 889
BspCNI CTCAG 1 cut(s) 517
BspDI ATCGAT 3 cut(s) 600, 708, 1128
BspFNI CGCG 1 cut(s) 1028
BspTI CTTAAG 1 cut(s) 647
BsrDI GCAATG 3 cut(s) 537, 580, 1042
BsrI ACTGG 1 cut(s) 1159
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 5 cut(s) 597, 765, 874, 1125, 1129
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 4 cut(s) 336, 458, 515, 983
Bst6I CTCTTC 2 cut(s) 5, 549
BstAFI CTTAAG 1 cut(s) 647
BstBAI YACGTR 1 cut(s) 1008
BstC8I GCNNGC 1 cut(s) 974
BstDEI CTNAG 2 cut(s) 525, 630
BstENI CCTNNNNNAGG 1 cut(s) 524
BstFNI CGCG 1 cut(s) 1028
BstHHI GCGC 1 cut(s) 1030
BstKTI GATC 5 cut(s) 600, 768, 877, 1128, 1132
BstMBI GATC 5 cut(s) 597, 765, 874, 1125, 1129
BstMWI GCNNNNNNNGC 2 cut(s) 110, 499
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 1 cut(s) 976
BstSCI CCNGG 2 cut(s) 411, 519
BstSLI GKGCMC 1 cut(s) 289
BstUI CGCG 1 cut(s) 1028
BstV2I GAAGAC 1 cut(s) 452
Bsu15I ATCGAT 3 cut(s) 600, 708, 1128
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 1 cut(s) 889
BsuTUI ATCGAT 3 cut(s) 600, 708, 1128
BtsIMutI CAGTG 2 cut(s) 988, 1058
Cac8I GCNNGC 1 cut(s) 974
CfoI GCGC 1 cut(s) 1030
ClaI ATCGAT 3 cut(s) 600, 708, 1128
CseI GACGC 1 cut(s) 110
Csp6I GTAC 4 cut(s) 126, 372, 511, 782
CviAII CATG 8 cut(s) 232, 497, 595, 815, 829, 878, 973, 1139
CviQI GTAC 4 cut(s) 126, 372, 511, 782
DdeI CTNAG 2 cut(s) 525, 630
DpnI GATC 5 cut(s) 599, 767, 876, 1127, 1131
DpnII GATC 5 cut(s) 597, 765, 874, 1125, 1129
DriI GACNNNNNGTC 1 cut(s) 365
Eam1104I CTCTTC 2 cut(s) 5, 549
Eam1105I GACNNNNNGTC 1 cut(s) 365
EarI CTCTTC 2 cut(s) 5, 549
Eco88I CYCGRG 1 cut(s) 36
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 1047
EcoRII CCWGG 1 cut(s) 519
FaeI CATG 8 cut(s) 235, 500, 598, 818, 832, 881, 976, 1142
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FatI CATG 8 cut(s) 231, 496, 594, 814, 828, 877, 972, 1138
FbaI TGATCA 1 cut(s) 765
GlaI GCGC 1 cut(s) 1029
GsaI CCCAGC 1 cut(s) 841
HaeIII GGCC 1 cut(s) 889
HapII CCGG 2 cut(s) 412, 785
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 1030
Hin1II CATG 8 cut(s) 235, 500, 598, 818, 832, 881, 976, 1142
Hin6I GCGC 1 cut(s) 1028
HinP1I GCGC 1 cut(s) 1028
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 9 cut(s) 76, 169, 175, 235, 643, 811, 832, 882, 1063
HpaII CCGG 2 cut(s) 412, 785
HphI GGTGA 1 cut(s) 892
Hpy166II GTNNAC 1 cut(s) 332
Hpy188I TCNGA 5 cut(s) 100, 160, 174, 359, 408
Hpy188III TCNNGA 6 cut(s) 36, 189, 640, 758, 964, 1052
Hpy8I GTNNAC 1 cut(s) 332
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 5 cut(s) 60, 553, 663, 857, 1145
HpyCH4III ACNGT 4 cut(s) 336, 458, 515, 983
HpyCH4IV ACGT 3 cut(s) 1007, 1090, 1104
HpyCH4V TGCA 9 cut(s) 183, 507, 573, 612, 825, 972, 1005, 1035, 1142
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 499
HpyF3I CTNAG 2 cut(s) 525, 630
HpySE526I ACGT 3 cut(s) 1007, 1090, 1104
Hsp92II CATG 8 cut(s) 235, 500, 598, 818, 832, 881, 976, 1142
HspAI GCGC 1 cut(s) 1028
Ksp22I TGATCA 1 cut(s) 765
Kzo9I GATC 5 cut(s) 597, 765, 874, 1125, 1129
LmnI GCTCC 2 cut(s) 535, 855
LweI GCATC 2 cut(s) 170, 730
MaeII ACGT 3 cut(s) 1007, 1090, 1104
MaeIII GTNAC 6 cut(s) 218, 344, 797, 898, 1108, 1150
MalI GATC 5 cut(s) 599, 767, 876, 1127, 1131
MboI GATC 5 cut(s) 597, 765, 874, 1125, 1129
MboII GAAGA 9 cut(s) 16, 22, 38, 452, 566, 664, 674, 854, 884
MfeI CAATTG 1 cut(s) 271
MhlI GDGCHC 2 cut(s) 289, 852
MlyI GAGTC 4 cut(s) 169, 841, 891, 1057
MmeI TCCRAC 4 cut(s) 123, 138, 382, 924
MnlI CCTC 8 cut(s) 6, 32, 39, 265, 520, 864, 933, 1152
MroXI GAANNNNTTC 1 cut(s) 1047
MseI TTAA 9 cut(s) 108, 138, 225, 450, 548, 648, 675, 914, 978
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 2 cut(s) 412, 785
MspR9I CCNGG 2 cut(s) 413, 521
MunI CAATTG 1 cut(s) 271
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 1 cut(s) 1028
MwoI GCNNNNNNNGC 2 cut(s) 110, 499
NciI CCSGG 1 cut(s) 413
NdeII GATC 5 cut(s) 597, 765, 874, 1125, 1129
NlaIII CATG 8 cut(s) 235, 500, 598, 818, 832, 881, 976, 1142
NmuCI GTSAC 2 cut(s) 898, 1108
NspI RCATGY 1 cut(s) 976
PaeI GCATGC 1 cut(s) 976
PaeR7I CTCGAG 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 1047
PfeI GAWTC 5 cut(s) 76, 169, 235, 643, 811
PflFI GACNNNGTC 1 cut(s) 1106
PleI GAGTC 4 cut(s) 169, 840, 890, 1057
PpsI GAGTC 4 cut(s) 169, 840, 890, 1057
Ppu21I YACGTR 1 cut(s) 1008
Psp6I CCWGG 1 cut(s) 519
PspFI CCCAGC 1 cut(s) 837
PspGI CCWGG 1 cut(s) 519
PsyI GACNNNGTC 1 cut(s) 1106
RsaI GTAC 4 cut(s) 127, 373, 512, 783
RsaNI GTAC 4 cut(s) 126, 372, 511, 782
SaqAI TTAA 9 cut(s) 108, 138, 225, 450, 548, 648, 675, 914, 978
Sau3AI GATC 5 cut(s) 597, 765, 874, 1125, 1129
SchI GAGTC 4 cut(s) 169, 841, 891, 1057
ScrFI CCNGG 2 cut(s) 413, 521
SduI GDGCHC 2 cut(s) 289, 852
SfaNI GCATC 2 cut(s) 170, 730
Sfr274I CTCGAG 1 cut(s) 36
SlaI CTCGAG 1 cut(s) 36
SmlI CTYRAG 2 cut(s) 36, 647
SmoI CTYRAG 2 cut(s) 36, 647
SphI GCATGC 1 cut(s) 976
SsiI CCGC 1 cut(s) 73
SspI AATATT 1 cut(s) 308
StyD4I CCNGG 2 cut(s) 411, 519
TaaI ACNGT 4 cut(s) 336, 458, 515, 983
TaiI ACGT 3 cut(s) 1010, 1093, 1107
TaqI TCGA 7 cut(s) 37, 178, 600, 639, 708, 757, 1128
TatI WGTACW 2 cut(s) 371, 510
TfiI GAWTC 5 cut(s) 76, 169, 235, 643, 811
Tru1I TTAA 9 cut(s) 108, 138, 225, 450, 548, 648, 675, 914, 978
Tru9I TTAA 9 cut(s) 108, 138, 225, 450, 548, 648, 675, 914, 978
TscAI CASTG 2 cut(s) 988, 1065
TseFI GTSAC 2 cut(s) 898, 1108
Tsp45I GTSAC 2 cut(s) 898, 1108
TspRI CASTG 2 cut(s) 988, 1065
Tth111I GACNNNGTC 1 cut(s) 1106
Vha464I CTTAAG 1 cut(s) 647
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 3 cut(s) 624, 987, 1047
XceI RCATGY 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 36
XmnI GAANNNNTTC 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.