MD05G1106300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000910
Physical Location & Seq
Forward (+)
22008010 .. 22009467
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1106300.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGAAACTGAGATTGCGATTGATAGAAACCAAAGAGACCATCCGAGTGGAGGTCCCCGATCTCTGCTCTCTCCACCACCTCAAACAAACCGTCTCCCAACTAATCTCCGCCGCTCCTTCCTCCCTCCGCCTCTCCCTCAACCGCCGCAACGAGCTCCATGCGCCATCCCCTCACGACCCCCTCCGCTCCCTCGGCATCACCTCCGGCGACCTTGTCTTCTACACTCTCGACCCCTCCTCCCAAGCCCTGGCTTCGCCTCTCCATCCAATTTCGCATTCGTCCGCAGAATCCCCAAATCGAACTGAGCCCCAGTGTTCGAATTTCCTAAACTCCGAAACCCTAATTGACGAAGGGGCTGTTTTGGCCGATTTAATTGCTGAAGAACAGAGGTCTGGAGGTTCGAATTCTGAAACCCTAGTGGTTTCTGGTGCCGAATGTATGGATATTGATGACCCGTCTGATGGGCTAGGGTTGAAGAAATACTCGGTTCCATTTTTCTTGAAGAGGGTACTGAGGGAAGAGTTGGGAGACGACCATAGCAATCACAAGCTATTGGTGATTGCTGTACATGCTGTGCTCTTAGAGTCTGGTTTCGTTGGCTTCGATTCGATTTCGGGTATGCTGGCCGATCAAAGGACGGGTTCAACGATTTCGCTTTCGTATACTCTGCCTGAGATATTGCGAAATCGGGGCAATACTGGTAAAGAGGTTGAAAGGGTTGTGTTGAAGTTTCAGAATTTGGGGCATTTTGTGAATGTTTATGGGTCTTTGGCAAATGGCGGTTCTTCGCCCTATCAGGTCTGTTTGGACAAAAACAGATTTGCCCCAACGATTGAATTCGTTTATGGAACCCAAAATGTCAATGACAGAGATGGGTTCGTTTTAGAGTTATCGAAGATTGTGAAAGATGGGATCACATTCCCATTGCTGATTGATCTTTGCGCAAAGGCTGGTTTGCCTGCACCACCGAGCCTTATGCGCCTTCCACCGGAGCTCAAAATGAAGATTCTGGAGCCACTGGCTGGTGTTGACATTGCCAAGAAGTACACTGAGGAGTTCGGTAATGGTAGTGGAGGAGAAGGAACTGGAGGTGAAGGAACAGTGACTAACTGGAAGTTTAGATTTGTTGGAAATTGGGAGACTAGGAAGCAACAAATGAAAGCAGTAGTGAGGTGGAGATCATTTGAGAGGCCTTATTTCAACCTTATCAGGAGGGACCCTAACCCATTTGTACCTCCAAGGATTGGTGGAATAATTGGTGGAGATTATGACCGATTTCCACTCTTTGGTGCAATTGATCCTACTGGACAAGCACACCCTCTTCTACAACAACCCCGAAGATCTGGTATGCGGCGTAATTTTTCACCCAATTGCGATCTGGGAGGGTTCAATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

466

Amino Acids

51.2

Weight (kDa)

6.62

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 943
AccB1I GGYRCC 1 cut(s) 428
AccB7I CCANNNNNTGG 4 cut(s) 247, 1022, 1244, 1286
AccBSI CCGCTC 2 cut(s) 113, 186
AccI GTMKAC 1 cut(s) 662
AciI CCGC 9 cut(s) 108, 111, 127, 142, 145, 184, 282, 780, 1351
AclWI GGATC 2 cut(s) 920, 1292
AcoI YGGCCR 2 cut(s) 363, 624
AcsI RAATTY 4 cut(s) 319, 403, 736, 836
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 4 cut(s) 510, 567, 1046, 1233
AfiI CCNNNNNNNGG 8 cut(s) 49, 247, 461, 633, 988, 1022, 1244, 1286
AgsI TTSAA 8 cut(s) 475, 502, 645, 713, 727, 836, 1201, 1390
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 259, 291
AluBI AGCT 3 cut(s) 154, 550, 994
AluI AGCT 3 cut(s) 154, 550, 994
Alw21I GWGCWC 3 cut(s) 156, 579, 996
Alw26I GTCTC 4 cut(s) 29, 97, 522, 1133
AlwI GGATC 2 cut(s) 920, 1292
AoxI GGCC 3 cut(s) 363, 624, 1190
ApoI RAATTY 4 cut(s) 319, 403, 736, 836
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
AspLEI GCGC 3 cut(s) 163, 944, 981
AspS9I GGNCC 2 cut(s) 52, 1216
AsuHPI GGTGA 4 cut(s) 190, 568, 1103, 1356
AsuII TTCGAA 2 cut(s) 317, 401
AvaII GGWCC 2 cut(s) 52, 1216
BanI GGYRCC 1 cut(s) 428
BanII GRGCYC 3 cut(s) 156, 309, 996
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 3 cut(s) 156, 579, 996
BccI CCATC 6 cut(s) 47, 172, 270, 455, 866, 902
BcgI CGANNNNNNTGC 6 cut(s) 140, 174, 356, 390, 958, 992
BciT130I CCWGG 1 cut(s) 248
BcoDI GTCTC 4 cut(s) 29, 97, 522, 1133
BfaI CTAG 3 cut(s) 416, 467, 1143
BglII AGATCT 1 cut(s) 1340
BisI GCNGC 3 cut(s) 111, 145, 1352
BlsI GCNGC 3 cut(s) 112, 146, 1353
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 2 cut(s) 52, 1216
BmgT120I GGNCC 2 cut(s) 52, 1216
BmiI GGNNCC 7 cut(s) 54, 430, 489, 850, 1014, 1217, 1218
BmrFI CCNGG 1 cut(s) 248
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 204
BmuI ACTGGG 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 3 cut(s) 414, 1031, 1107
Bpu14I TTCGAA 2 cut(s) 317, 401
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 190, 246, 1238
BsaWI WCCGGW 1 cut(s) 988
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 8 cut(s) 49, 247, 461, 633, 988, 1022, 1244, 1286
Bse1I ACTGG 6 cut(s) 310, 703, 1023, 1090, 1115, 1309
Bse3DI GCAATG 2 cut(s) 923, 1032
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 3 cut(s) 190, 246, 1238
BseGI GGATG 3 cut(s) 39, 164, 262
BseLI CCNNNNNNNGG 8 cut(s) 49, 247, 461, 633, 988, 1022, 1244, 1286
BseMI GCAATG 2 cut(s) 923, 1032
BseMII CTCAG 4 cut(s) 294, 503, 663, 1041
BseNI ACTGG 6 cut(s) 310, 703, 1023, 1090, 1115, 1309
BseRI GAGGAG 3 cut(s) 226, 1067, 1089
BsgI GTGCAG 1 cut(s) 945
BshFI GGCC 3 cut(s) 365, 626, 1192
BshNI GGYRCC 1 cut(s) 428
BsiHKAI GWGCWC 3 cut(s) 156, 579, 996
BsiSI CCGG 2 cut(s) 204, 989
BslFI GGGAC 2 cut(s) 38, 1229
BslI CCNNNNNNNGG 8 cut(s) 49, 247, 461, 633, 988, 1022, 1244, 1286
BsmAI GTCTC 4 cut(s) 29, 97, 522, 1133
BsmBI CGTCTC 2 cut(s) 97, 522
BsmFI GGGAC 2 cut(s) 38, 1229
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 3 cut(s) 365, 626, 1192
Bso31I GGTCTC 1 cut(s) 29
Bsp119I TTCGAA 2 cut(s) 317, 401
Bsp1286I GDGCHC 4 cut(s) 156, 309, 579, 996
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
BspACI CCGC 9 cut(s) 108, 111, 127, 142, 145, 184, 282, 780, 1351
BspANI GGCC 3 cut(s) 365, 626, 1192
BspCNI CTCAG 4 cut(s) 295, 504, 664, 1042
BspLI GGNNCC 7 cut(s) 54, 430, 489, 850, 1014, 1217, 1218
BspPI GGATC 2 cut(s) 920, 1292
BspT104I TTCGAA 2 cut(s) 317, 401
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 29
BsrBI CCGCTC 2 cut(s) 113, 186
BsrDI GCAATG 2 cut(s) 923, 1032
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 6 cut(s) 310, 703, 1023, 1090, 1115, 1309
BssECI CCNNGG 3 cut(s) 190, 246, 1238
BssMI GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
BssNAI GTATAC 1 cut(s) 663
BssT1I CCWWGG 1 cut(s) 1238
Bst1107I GTATAC 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 2 cut(s) 91, 1102
Bst6I CTCTTC 3 cut(s) 497, 513, 1326
BstAUI TGTACA 1 cut(s) 565
BstBI TTCGAA 2 cut(s) 317, 401
BstC8I GCNNGC 2 cut(s) 624, 960
BstDEI CTNAG 6 cut(s) 8, 303, 512, 580, 672, 1050
BstF5I GGATG 3 cut(s) 39, 164, 262
BstHHI GCGC 3 cut(s) 163, 944, 981
BstKTI GATC 8 cut(s) 61, 631, 915, 937, 1181, 1300, 1343, 1378
BstMAI GTCTC 4 cut(s) 29, 97, 522, 1133
BstMBI GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
BstMWI GCNNNNNNNGC 5 cut(s) 160, 192, 362, 569, 978
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 1 cut(s) 246
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 1340
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 1 cut(s) 1340
BstZ17I GTATAC 1 cut(s) 663
BsuRI GGCC 3 cut(s) 365, 626, 1192
BtsCI GGATG 3 cut(s) 39, 164, 262
BtsIMutI CAGTG 4 cut(s) 317, 1016, 1047, 1107
Cac8I GCNNGC 2 cut(s) 624, 960
CfoI GCGC 3 cut(s) 163, 944, 981
Cfr13I GGNCC 2 cut(s) 52, 1216
Csp6I GTAC 4 cut(s) 509, 566, 1045, 1232
CviAII CATG 2 cut(s) 158, 569
CviQI GTAC 4 cut(s) 509, 566, 1045, 1232
DdeI CTNAG 6 cut(s) 8, 303, 512, 580, 672, 1050
DpnI GATC 8 cut(s) 60, 630, 914, 936, 1180, 1299, 1342, 1377
DpnII GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
EaeI YGGCCR 2 cut(s) 363, 624
Eam1104I CTCTTC 3 cut(s) 497, 513, 1326
EarI CTCTTC 3 cut(s) 497, 513, 1326
EciI GGCGGA 2 cut(s) 97, 116
Ecl136II GAGCTC 2 cut(s) 154, 994
Eco130I CCWWGG 1 cut(s) 1238
Eco147I AGGCCT 1 cut(s) 1192
Eco24I GRGCYC 3 cut(s) 156, 309, 996
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 2 cut(s) 52, 1216
Eco53kI GAGCTC 2 cut(s) 154, 994
Eco57I CTGAAG 1 cut(s) 399
EcoICRI GAGCTC 2 cut(s) 154, 994
EcoO109I RGGNCCY 2 cut(s) 52, 1216
EcoRI GAATTC 2 cut(s) 403, 836
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 1238
EcoT38I GRGCYC 3 cut(s) 156, 309, 996
ErhI CCWWGG 1 cut(s) 1238
Esp3I CGTCTC 2 cut(s) 97, 522
FaeI CATG 2 cut(s) 161, 572
FaqI GGGAC 2 cut(s) 38, 1229
FatI CATG 2 cut(s) 157, 568
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 3 cut(s) 111, 145, 1352
FokI GGATG 3 cut(s) 26, 151, 249
FriOI GRGCYC 3 cut(s) 156, 309, 996
Fsp4HI GCNGC 3 cut(s) 111, 145, 1352
FspBI CTAG 3 cut(s) 416, 467, 1143
FspI TGCGCA 1 cut(s) 943
GlaI GCGC 3 cut(s) 162, 943, 980
GluI GCNGC 3 cut(s) 111, 145, 1352
GsuI CTGGAG 3 cut(s) 414, 1031, 1107
HaeIII GGCC 3 cut(s) 365, 626, 1192
HapII CCGG 2 cut(s) 204, 989
HhaI GCGC 3 cut(s) 163, 944, 981
Hin1II CATG 2 cut(s) 161, 572
Hin6I GCGC 3 cut(s) 161, 942, 979
HinP1I GCGC 3 cut(s) 161, 942, 979
HincII GTYRAC 1 cut(s) 1030
HindII GTYRAC 1 cut(s) 1030
HinfI GANTC 4 cut(s) 287, 584, 605, 1006
HpaII CCGG 2 cut(s) 204, 989
HphI GGTGA 4 cut(s) 190, 568, 1103, 1356
Hpy166II GTNNAC 3 cut(s) 663, 1030, 1047
Hpy188I TCNGA 6 cut(s) 44, 334, 409, 460, 735, 1397
Hpy188III TCNNGA 6 cut(s) 173, 227, 393, 499, 1010, 1210
Hpy8I GTNNAC 3 cut(s) 663, 1030, 1047
HpyAV CCTTC 5 cut(s) 126, 344, 992, 1073, 1088
HpyCH4III ACNGT 2 cut(s) 91, 1102
HpyCH4V TGCA 2 cut(s) 962, 1292
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 192, 362, 569, 978
HpyF3I CTNAG 6 cut(s) 8, 303, 512, 580, 672, 1050
Hsp92II CATG 2 cut(s) 161, 572
HspAI GCGC 3 cut(s) 161, 942, 979
KflI GGGWCCC 1 cut(s) 1216
Kzo9I GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
LmnI GCTCC 5 cut(s) 118, 159, 191, 991, 1012
LweI GCATC 1 cut(s) 204
MaeI CTAG 3 cut(s) 416, 467, 1143
MaeIII GTNAC 1 cut(s) 1102
MalI GATC 8 cut(s) 60, 630, 914, 936, 1180, 1299, 1342, 1377
MbiI CCGCTC 2 cut(s) 113, 186
MboI GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
MfeI CAATTG 2 cut(s) 1293, 1369
MflI RGATCY 1 cut(s) 1340
MhlI GDGCHC 4 cut(s) 156, 309, 579, 996
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 1108
MseI TTAA 1 cut(s) 371
MslI CAYNNNNRTG 1 cut(s) 44
MspI CCGG 2 cut(s) 204, 989
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 2 cut(s) 1293, 1369
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 5 cut(s) 160, 192, 362, 569, 978
NdeII GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
NlaIII CATG 2 cut(s) 161, 572
NlaIV GGNNCC 7 cut(s) 54, 430, 489, 850, 1014, 1217, 1218
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 1102
NsbI TGCGCA 1 cut(s) 943
NspI RCATGY 1 cut(s) 572
NspV TTCGAA 2 cut(s) 317, 401
PceI AGGCCT 1 cut(s) 1192
PcsI WCGNNNNNNNCGW 2 cut(s) 600, 644
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 3 cut(s) 287, 605, 1006
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 4 cut(s) 247, 1022, 1244, 1286
PkrI GCNGC 3 cut(s) 112, 146, 1353
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 2 cut(s) 52, 1216
Psp124BI GAGCTC 2 cut(s) 156, 996
Psp5II RGGWCCY 2 cut(s) 52, 1216
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 7 cut(s) 54, 430, 489, 850, 1014, 1217, 1218
PspPI GGNCC 2 cut(s) 52, 1216
PspPPI RGGWCCY 2 cut(s) 52, 1216
PsuI RGATCY 1 cut(s) 1340
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 4 cut(s) 510, 567, 1046, 1233
RsaNI GTAC 4 cut(s) 509, 566, 1045, 1232
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 2 cut(s) 156, 996
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 3 cut(s) 111, 145, 1352
Sau3AI GATC 8 cut(s) 58, 628, 912, 934, 1178, 1297, 1340, 1375
Sau96I GGNCC 2 cut(s) 52, 1216
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 4 cut(s) 156, 309, 579, 996
SfaNI GCATC 1 cut(s) 204
SfuI TTCGAA 2 cut(s) 317, 401
SinI GGWCC 2 cut(s) 52, 1216
SmiMI CAYNNNNRTG 1 cut(s) 44
SseBI AGGCCT 1 cut(s) 1192
SsiI CCGC 9 cut(s) 108, 111, 127, 142, 145, 184, 282, 780, 1351
SspMI CTAG 3 cut(s) 416, 467, 1143
SstI GAGCTC 2 cut(s) 156, 996
StuI AGGCCT 1 cut(s) 1192
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 1 cut(s) 1238
TaaI ACNGT 2 cut(s) 91, 1102
TaqI TCGA 7 cut(s) 228, 298, 317, 401, 603, 608, 893
TaqII GACCGA 1 cut(s) 1287
TatI WGTACW 2 cut(s) 565, 1044
TauI GCSGC 3 cut(s) 113, 147, 1354
TfiI GAWTC 3 cut(s) 287, 605, 1006
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TscAI CASTG 4 cut(s) 317, 1023, 1054, 1107
TseFI GTSAC 1 cut(s) 1102
Tsp45I GTSAC 1 cut(s) 1102
TspDTI ATGAA 3 cut(s) 17, 1016, 1172
TspRI CASTG 4 cut(s) 317, 1023, 1054, 1107
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 4 cut(s) 247, 1022, 1244, 1286
VpaK11BI GGWCC 2 cut(s) 52, 1216
XapI RAATTY 4 cut(s) 319, 403, 736, 836
XceI RCATGY 1 cut(s) 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 1375
XmiI GTMKAC 1 cut(s) 662
XspI CTAG 3 cut(s) 416, 467, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.