MD10G1110300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000910
Physical Location & Seq
Forward (+)
18160356 .. 18161132
777 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1110300.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGAAATCCATGATGGATCTAGTGGGCTTGTGGGGAACAACAAGAAGTACTCAATACTCTTTTTCTTGATGAAGGTGTTGAGGGAGCTGTTGTTAGAAGACCGTAGCTTTCACAAGTTGTTGGTGATTGCAGTGCATGCAGTGTTTCTAGAGTTCGGTTTCGTCCAATTCGACTCATTTTCGGGTGCCCAAGTCGATCGCTTTCATCTCTCAGACGAGTGGCCAACGACACCTTTCACGTTGTCGTTTACCTACACTATGCCCGAAATTCTAAAAAATCGAAATAATTGTGTTTACGTTTTCGGGTCTTTAATTGGAATAGACGGCGTCCTAATAAGATTTCAGAAGGTGTGCAACTCTGTCTACGTTTTCGGGTCTTTAATTGGAAGCGGGTCGGGTTCTTACTGGGTACAATTGAACCCCGAGAGGTTTGGGCCCATGATCCGAATGCTGAACGACACAAAAGGACTTGTCGCTAAAGAAGAAGTTTTGGAACTGTGGAAAATTGTGAAGGACGAGATTGTGCTGCCGTTGGCGATCGATCTTTGTGCGAATGCTGGTTTGCCAGCTCCGCCGAGCTTCATGCAGCTTCCGTCGGAGATTCAAATGAAGATTTTGAGGTTGTTGCCCGGTGTGGATATTGCCATGGTGGGTTGTGTGTGTAAGGAATTGAGGGATCTTGCTAACGATGACGAACTGTGGAAGCAGAAGGTTTTTGATGAGTTTACTGTTGAGTTTCTTCTTGATCAAACCTCAAGCATTATCAGTTTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

259

Amino Acids

29.11

Weight (kDa)

5.07

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 195 - 235 5.9e-08 F-box domain
F-box-like PF12937 196 - 238 2.2e-11 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 363
AciI CCGC 2 cut(s) 390, 572
AclWI GGATC 3 cut(s) 25, 436, 684
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 2 cut(s) 50, 411
AgsI TTSAA 2 cut(s) 418, 605
AluBI AGCT 5 cut(s) 88, 108, 569, 579, 589
AluI AGCT 5 cut(s) 88, 108, 569, 579, 589
AlwI GGATC 3 cut(s) 25, 436, 684
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 2 cut(s) 221, 434
ApaI GGGCCC 1 cut(s) 438
ApeKI GCWGC 2 cut(s) 526, 586
ApoI RAATTY 1 cut(s) 267
AspS9I GGNCC 2 cut(s) 434, 435
AsuC2I CCSGG 1 cut(s) 630
AsuHPI GGTGA 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 422
BaeGI GKGCMC 2 cut(s) 190, 438
BalI TGGCCA 1 cut(s) 223
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 513, 598
BccI CCATC 1 cut(s) 8
BceAI ACGGC 2 cut(s) 340, 514
BcgI CGANNNNNNTGC 4 cut(s) 530, 564, 565, 599
BclI TGATCA 1 cut(s) 745
BcnI CCSGG 1 cut(s) 630
BfaI CTAG 2 cut(s) 21, 149
BisI GCNGC 2 cut(s) 527, 587
BlsI GCNGC 2 cut(s) 528, 588
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 1 cut(s) 630
BmeT110I CYCGRG 1 cut(s) 422
BmgT120I GGNCC 2 cut(s) 434, 435
BmiI GGNNCC 2 cut(s) 187, 436
BmrFI CCNGG 1 cut(s) 630
BmrI ACTGGG 1 cut(s) 415
BmuI ACTGGG 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 739
BpuMI CCSGG 1 cut(s) 630
Bsa29I ATCGAT 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 645
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bse1I ACTGG 1 cut(s) 410
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 645
BseMII CTCAG 1 cut(s) 225
BseNI ACTGG 1 cut(s) 410
BseSI GKGCMC 2 cut(s) 190, 438
BseXI GCAGC 2 cut(s) 513, 598
Bsh1285I CGRYCG 2 cut(s) 199, 540
BshFI GGCC 2 cut(s) 223, 436
BshNI GGYRCC 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 540
BsiEI CGRYCG 2 cut(s) 199, 540
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 1 cut(s) 630
BsmI GAATGC 2 cut(s) 453, 559
BsnI GGCC 2 cut(s) 223, 436
BsoBI CYCGRG 1 cut(s) 422
Bsp120I GGGCCC 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 190, 438
Bsp143I GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
Bsp19I CCATGG 1 cut(s) 645
BspACI CCGC 2 cut(s) 390, 572
BspANI GGCC 2 cut(s) 223, 436
BspCNI CTCAG 1 cut(s) 224
BspDI ATCGAT 1 cut(s) 540
BspLI GGNNCC 2 cut(s) 187, 436
BspPI GGATC 3 cut(s) 25, 436, 684
BspT107I GGYRCC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 410
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 645
Bst4CI ACNGT 4 cut(s) 104, 498, 699, 730
BstACI GRCGYC 1 cut(s) 327
BstAPI GCANNNNNTGC 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 138, 567
BstDEI CTNAG 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 645
BstKTI GATC 7 cut(s) 20, 199, 444, 540, 544, 679, 748
BstMBI GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
BstMCI CGRYCG 2 cut(s) 199, 540
BstMWI GCNNNNNNNGC 2 cut(s) 137, 571
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 628
BstSLI GKGCMC 2 cut(s) 190, 438
BstV1I GCAGC 2 cut(s) 513, 598
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 17, 676
BstYI RGATCY 2 cut(s) 17, 676
Bsu15I ATCGAT 1 cut(s) 540
BsuRI GGCC 2 cut(s) 223, 436
BsuTUI ATCGAT 1 cut(s) 540
BtgI CCRYGG 1 cut(s) 645
BtsI GCAGTG 2 cut(s) 138, 147
BtsIMutI CAGTG 2 cut(s) 138, 147
Cac8I GCNNGC 2 cut(s) 138, 567
Cfr13I GGNCC 2 cut(s) 434, 435
ClaI ATCGAT 1 cut(s) 540
CseI GACGC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 49, 410
CviAII CATG 5 cut(s) 11, 137, 439, 583, 646
CviJI RGCY 8 cut(s) 28, 88, 108, 223, 436, 569, 579, 589
CviKI_1 RGCY 8 cut(s) 28, 88, 108, 223, 436, 569, 579, 589
CviQI GTAC 2 cut(s) 49, 410
DdeI CTNAG 1 cut(s) 211
DpnI GATC 7 cut(s) 19, 198, 443, 539, 543, 678, 747
DpnII GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
EaeI YGGCCR 1 cut(s) 221
EciI GGCGGA 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 645
Eco24I GRGCYC 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 645
EcoT38I GRGCYC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 5 cut(s) 14, 140, 442, 586, 649
FaiI YATR 6 cut(s) 12, 138, 260, 440, 584, 647
FatI CATG 5 cut(s) 10, 136, 438, 582, 645
FauI CCCGC 1 cut(s) 383
FbaI TGATCA 1 cut(s) 745
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 2 cut(s) 527, 587
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 2 cut(s) 527, 587
FspBI CTAG 2 cut(s) 21, 149
GluI GCNGC 2 cut(s) 527, 587
HaeIII GGCC 2 cut(s) 223, 436
HapII CCGG 1 cut(s) 630
HgaI GACGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 5 cut(s) 14, 140, 442, 586, 649
HinfI GANTC 2 cut(s) 173, 601
HpaII CCGG 1 cut(s) 630
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 4 cut(s) 249, 295, 364, 726
Hpy188I TCNGA 4 cut(s) 214, 345, 446, 598
Hpy188III TCNNGA 3 cut(s) 67, 149, 743
Hpy8I GTNNAC 4 cut(s) 249, 295, 364, 726
Hpy99I CGWCG 1 cut(s) 598
HpyAV CCTTC 4 cut(s) 67, 340, 505, 703
HpyCH4III ACNGT 4 cut(s) 104, 498, 699, 730
HpyCH4IV ACGT 3 cut(s) 239, 297, 366
HpyCH4V TGCA 5 cut(s) 131, 136, 140, 354, 586
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 571
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 3 cut(s) 239, 297, 366
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 5 cut(s) 14, 140, 442, 586, 649
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
LmnI GCTCC 2 cut(s) 85, 574
LpnPI CCDG 4 cut(s) 391, 543, 579, 643
Lsp1109I GCAGC 2 cut(s) 513, 598
MaeI CTAG 2 cut(s) 21, 149
MaeII ACGT 3 cut(s) 239, 297, 366
MalI GATC 7 cut(s) 19, 198, 443, 539, 543, 678, 747
MboI GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
MboII GAAGA 4 cut(s) 110, 494, 622, 731
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 2 cut(s) 17, 676
MhlI GDGCHC 2 cut(s) 190, 438
MlsI TGGCCA 1 cut(s) 223
MluCI AATT 8 cut(s) 167, 267, 286, 312, 381, 413, 504, 668
MluNI TGGCCA 1 cut(s) 223
MlyI GAGTC 1 cut(s) 167
MmeI TCCRAC 1 cut(s) 576
MnlI CCTC 5 cut(s) 75, 420, 612, 666, 763
Mox20I TGGCCA 1 cut(s) 223
MscI TGGCCA 1 cut(s) 223
MseI TTAA 2 cut(s) 311, 380
Msp20I TGGCCA 1 cut(s) 223
MspI CCGG 1 cut(s) 630
MspR9I CCNGG 1 cut(s) 630
MunI CAATTG 1 cut(s) 413
Mva1269I GAATGC 2 cut(s) 453, 559
MwoI GCNNNNNNNGC 2 cut(s) 137, 571
NciI CCSGG 1 cut(s) 630
NcoI CCATGG 1 cut(s) 645
NdeII GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
NlaIII CATG 5 cut(s) 14, 140, 442, 586, 649
NlaIV GGNNCC 2 cut(s) 187, 436
NmeAIII GCCGAG 1 cut(s) 600
NspI RCATGY 1 cut(s) 140
PaeI GCATGC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 2 cut(s) 453, 559
PfeI GAWTC 1 cut(s) 601
PflFI GACNNNGTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 528, 588
Ple19I CGATCG 2 cut(s) 199, 540
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PspN4I GGNNCC 2 cut(s) 187, 436
PspOMI GGGCCC 1 cut(s) 434
PspPI GGNCC 2 cut(s) 434, 435
PsuI RGATCY 2 cut(s) 17, 676
PsyI GACNNNGTC 1 cut(s) 326
PvuI CGATCG 2 cut(s) 199, 540
RsaI GTAC 2 cut(s) 50, 411
RsaNI GTAC 2 cut(s) 49, 410
SaqAI TTAA 2 cut(s) 311, 380
SatI GCNGC 2 cut(s) 527, 587
Sau3AI GATC 7 cut(s) 17, 196, 441, 537, 541, 676, 745
Sau96I GGNCC 2 cut(s) 434, 435
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 630
SduI GDGCHC 2 cut(s) 190, 438
SmlI CTYRAG 1 cut(s) 754
SmoI CTYRAG 1 cut(s) 754
SphI GCATGC 1 cut(s) 140
Sse9I AATT 8 cut(s) 167, 267, 286, 312, 381, 413, 504, 668
SsiI CCGC 2 cut(s) 390, 572
SspMI CTAG 2 cut(s) 21, 149
StyD4I CCNGG 1 cut(s) 628
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 4 cut(s) 104, 498, 699, 730
TaiI ACGT 3 cut(s) 242, 300, 369
TaqI TCGA 4 cut(s) 171, 195, 280, 540
TasI AATT 8 cut(s) 167, 267, 286, 312, 381, 413, 504, 668
TatI WGTACW 1 cut(s) 48
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 2 cut(s) 311, 380
Tru9I TTAA 2 cut(s) 311, 380
TscAI CASTG 2 cut(s) 138, 147
TseI GCWGC 2 cut(s) 526, 586
TspDTI ATGAA 4 cut(s) 86, 194, 571, 623
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 2 cut(s) 138, 147
Tth111I GACNNNGTC 1 cut(s) 326
XapI RAATTY 1 cut(s) 267
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 363
XspI CTAG 2 cut(s) 21, 149
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.