pycom10g09580

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000910
Physical Location & Seq
Forward (+)
12682952 .. 12683707
756 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g09580.1

Sequence Viewer

Length: 756 bp
ATGGAAATCCATGATGGATCTAGTGGGCTTGTGGGGAACAACAAGAAGTACTCAATACTCTTTTTCTTGATGAAGGTATTGAGGGAGGTGTTGTTAGAAGACCGTAGCTTTGACAAGTTGTTGGTGATTGTAGTGCATGCAGTGTTTCTAGAGTTCGGTTTCGTCCAATTCGATTCATTTTCGGGTGCCCAAGTCGATAGCTTTCATCTCTCAGACAAGTGGCCAACGACATCTTTCACGCTGTCGTTTACCTACACTATGCCCGAAATTCTGAAAAATCGAAATAATTGTGTCTACGCTTTCGGGTCTTTAATTGGAATAGACGGCGTCCTAATAAGATTTCAGAAGGTGTACAACTCTGTCTACGTTTTCGGGTCTTTAATTGGAAGCGGGTCGGGTTCTTACTGGGTACAATTGAACCTCGAGAGGTTTGGGCCCATGATCCGAATGCTGAACGACACAAAAGGACATGTCGCTAAAGAAGAAGTTTTGGAACTGTGGAAAATTGTGAAGGACGAGATTGTGCTGCCATTGGCAATTGATCTTTGTGCAAATGCTGGTTTGCCAGCTCCGCCGAGCTTCATGCAGCTTCCGTCGGAGATTCAAATGAAGATTTTGAGGTTGTTACCCGGTGTGGATATTGCCATGGTAGGTTGTGTGTGTAAGGAATTAAGGGATCTTGCTAACGATGACGAACTGTGGAAGCAGAAGGTTTTTGATGAGTTTATTGTTGAGTTTCTTCTTGCTCAAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

252

Amino Acids

28.37

Weight (kDa)

5.16

Isoelectric Point (pI)

28.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 195 - 235 5.7e-08 F-box domain
F-box-like PF12937 196 - 238 2.1e-11 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AccI GTMKAC 2 cut(s) 294, 363
AciI CCGC 2 cut(s) 390, 572
AclWI GGATC 3 cut(s) 25, 436, 684
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 3 cut(s) 50, 353, 411
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 2 cut(s) 418, 605
AluBI AGCT 5 cut(s) 108, 201, 569, 579, 589
AluI AGCT 5 cut(s) 108, 201, 569, 579, 589
AlwI GGATC 3 cut(s) 25, 436, 684
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 2 cut(s) 221, 434
ApaI GGGCCC 1 cut(s) 438
ApeKI GCWGC 2 cut(s) 526, 586
ApoI RAATTY 1 cut(s) 267
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
AspS9I GGNCC 2 cut(s) 434, 435
AsuC2I CCSGG 1 cut(s) 630
AsuHPI GGTGA 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 422
BaeGI GKGCMC 2 cut(s) 190, 438
BalI TGGCCA 1 cut(s) 223
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 513, 598
BccI CCATC 1 cut(s) 8
BceAI ACGGC 1 cut(s) 340
BcgI CGANNNNNNTGC 2 cut(s) 565, 599
BcnI CCSGG 1 cut(s) 630
BfaI CTAG 2 cut(s) 21, 149
BisI GCNGC 2 cut(s) 527, 587
BlsI GCNGC 2 cut(s) 528, 588
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 1 cut(s) 630
BmeT110I CYCGRG 1 cut(s) 422
BmgT120I GGNCC 2 cut(s) 434, 435
BmiI GGNNCC 2 cut(s) 187, 436
BmrFI CCNGG 1 cut(s) 630
BmrI ACTGGG 1 cut(s) 415
BmuI ACTGGG 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 630
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 645
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bse1I ACTGG 1 cut(s) 410
BseDI CCNNGG 1 cut(s) 645
BseMII CTCAG 1 cut(s) 225
BseNI ACTGG 1 cut(s) 410
BseSI GKGCMC 2 cut(s) 190, 438
BseXI GCAGC 2 cut(s) 513, 598
BshFI GGCC 2 cut(s) 223, 436
BshNI GGYRCC 1 cut(s) 185
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 1 cut(s) 630
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 2 cut(s) 223, 436
BsoBI CYCGRG 1 cut(s) 422
Bsp120I GGGCCC 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 190, 438
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 4 cut(s) 17, 441, 541, 676
Bsp19I CCATGG 1 cut(s) 645
BspACI CCGC 2 cut(s) 390, 572
BspANI GGCC 2 cut(s) 223, 436
BspCNI CTCAG 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 187, 436
BspPI GGATC 3 cut(s) 25, 436, 684
BspT107I GGYRCC 1 cut(s) 185
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 1 cut(s) 410
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 4 cut(s) 17, 441, 541, 676
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 645
Bst4CI ACNGT 3 cut(s) 104, 498, 699
BstACI GRCGYC 1 cut(s) 327
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 138, 567
BstDEI CTNAG 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 645
BstKTI GATC 4 cut(s) 20, 444, 544, 679
BstMBI GATC 4 cut(s) 17, 441, 541, 676
BstMWI GCNNNNNNNGC 1 cut(s) 571
BstNSI RCATGY 2 cut(s) 140, 473
BstSCI CCNGG 1 cut(s) 628
BstSLI GKGCMC 2 cut(s) 190, 438
BstV1I GCAGC 2 cut(s) 513, 598
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 17, 676
BstYI RGATCY 2 cut(s) 17, 676
BsuRI GGCC 2 cut(s) 223, 436
BtgI CCRYGG 1 cut(s) 645
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 138, 567
Cfr13I GGNCC 2 cut(s) 434, 435
CseI GACGC 1 cut(s) 316
Csp6I GTAC 3 cut(s) 49, 352, 410
CviAII CATG 6 cut(s) 11, 137, 439, 470, 583, 646
CviJI RGCY 8 cut(s) 28, 108, 201, 223, 436, 569, 579, 589
CviKI_1 RGCY 8 cut(s) 28, 108, 201, 223, 436, 569, 579, 589
CviQI GTAC 3 cut(s) 49, 352, 410
DdeI CTNAG 1 cut(s) 211
DpnI GATC 4 cut(s) 19, 443, 543, 678
DpnII GATC 4 cut(s) 17, 441, 541, 676
EaeI YGGCCR 1 cut(s) 221
EciI GGCGGA 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 645
Eco24I GRGCYC 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 645
EcoT38I GRGCYC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 6 cut(s) 14, 140, 442, 473, 586, 649
FaiI YATR 7 cut(s) 12, 138, 260, 440, 471, 584, 647
FatI CATG 6 cut(s) 10, 136, 438, 469, 582, 645
FauI CCCGC 1 cut(s) 383
FblI GTMKAC 2 cut(s) 294, 363
Fnu4HI GCNGC 2 cut(s) 527, 587
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 2 cut(s) 527, 587
FspBI CTAG 2 cut(s) 21, 149
GluI GCNGC 2 cut(s) 527, 587
HaeIII GGCC 2 cut(s) 223, 436
HapII CCGG 1 cut(s) 630
HgaI GACGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 6 cut(s) 14, 140, 442, 473, 586, 649
HinfI GANTC 2 cut(s) 173, 601
HpaII CCGG 1 cut(s) 630
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 4 cut(s) 249, 295, 352, 364
Hpy188I TCNGA 5 cut(s) 214, 273, 345, 446, 598
Hpy188III TCNNGA 3 cut(s) 67, 149, 424
Hpy8I GTNNAC 4 cut(s) 249, 295, 352, 364
Hpy99I CGWCG 1 cut(s) 598
HpyAV CCTTC 4 cut(s) 67, 340, 505, 703
HpyCH4III ACNGT 3 cut(s) 104, 498, 699
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 136, 140, 551, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 571
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 1 cut(s) 366
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 6 cut(s) 14, 140, 442, 473, 586, 649
Kzo9I GATC 4 cut(s) 17, 441, 541, 676
LmnI GCTCC 1 cut(s) 574
LpnPI CCDG 4 cut(s) 391, 543, 579, 643
Lsp1109I GCAGC 2 cut(s) 513, 598
MaeI CTAG 2 cut(s) 21, 149
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 1 cut(s) 624
MalI GATC 4 cut(s) 19, 443, 543, 678
MboI GATC 4 cut(s) 17, 441, 541, 676
MboII GAAGA 4 cut(s) 110, 494, 622, 731
MfeI CAATTG 2 cut(s) 413, 537
MflI RGATCY 2 cut(s) 17, 676
MhlI GDGCHC 2 cut(s) 190, 438
MlsI TGGCCA 1 cut(s) 223
MluCI AATT 9 cut(s) 167, 267, 286, 312, 381, 413, 504, 537, 668
MluNI TGGCCA 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 576
MnlI CCTC 5 cut(s) 75, 79, 420, 431, 612
Mox20I TGGCCA 1 cut(s) 223
MscI TGGCCA 1 cut(s) 223
MseI TTAA 3 cut(s) 311, 380, 671
Msp20I TGGCCA 1 cut(s) 223
MspI CCGG 1 cut(s) 630
MspR9I CCNGG 1 cut(s) 630
MunI CAATTG 2 cut(s) 413, 537
Mva1269I GAATGC 1 cut(s) 453
MwoI GCNNNNNNNGC 1 cut(s) 571
NciI CCSGG 1 cut(s) 630
NcoI CCATGG 1 cut(s) 645
NdeII GATC 4 cut(s) 17, 441, 541, 676
NlaIII CATG 6 cut(s) 14, 140, 442, 473, 586, 649
NlaIV GGNNCC 2 cut(s) 187, 436
NmeAIII GCCGAG 1 cut(s) 600
NspI RCATGY 2 cut(s) 140, 473
PaeI GCATGC 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 422
PciI ACATGT 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 2 cut(s) 173, 601
PflFI GACNNNGTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 528, 588
PscI ACATGT 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 187, 436
PspOMI GGGCCC 1 cut(s) 434
PspPI GGNCC 2 cut(s) 434, 435
PsuI RGATCY 2 cut(s) 17, 676
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 3 cut(s) 50, 353, 411
RsaNI GTAC 3 cut(s) 49, 352, 410
SaqAI TTAA 3 cut(s) 311, 380, 671
SatI GCNGC 2 cut(s) 527, 587
Sau3AI GATC 4 cut(s) 17, 441, 541, 676
Sau96I GGNCC 2 cut(s) 434, 435
ScaI AGTACT 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 630
SduI GDGCHC 2 cut(s) 190, 438
Sfr274I CTCGAG 1 cut(s) 422
SlaI CTCGAG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SphI GCATGC 1 cut(s) 140
Sse9I AATT 9 cut(s) 167, 267, 286, 312, 381, 413, 504, 537, 668
SsiI CCGC 2 cut(s) 390, 572
SspMI CTAG 2 cut(s) 21, 149
StyD4I CCNGG 1 cut(s) 628
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 3 cut(s) 104, 498, 699
TaiI ACGT 1 cut(s) 369
TaqI TCGA 4 cut(s) 171, 195, 280, 423
TasI AATT 9 cut(s) 167, 267, 286, 312, 381, 413, 504, 537, 668
TatI WGTACW 2 cut(s) 48, 351
TfiI GAWTC 2 cut(s) 173, 601
Tru1I TTAA 3 cut(s) 311, 380, 671
Tru9I TTAA 3 cut(s) 311, 380, 671
TscAI CASTG 1 cut(s) 147
TseI GCWGC 2 cut(s) 526, 586
TspDTI ATGAA 5 cut(s) 86, 165, 194, 571, 623
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 1 cut(s) 147
Tth111I GACNNNGTC 1 cut(s) 326
XapI RAATTY 1 cut(s) 267
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 2 cut(s) 140, 473
XhoI CTCGAG 1 cut(s) 422
XmiI GTMKAC 2 cut(s) 294, 363
XspI CTAG 2 cut(s) 21, 149
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.